AT1G61330

FBD-associated F-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0016662
Physical Location & Seq
Forward (+)
22622975 .. 22624527
1553 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G61330.1

Sequence Viewer

Length: 1344 bp
ATGTCAGAAGCGTCTAATAAGCAGATGAAACTCATCAAAAGATTGTCTGATGAGTTGGTGGAGTGCATCTTGTCATTTCTTCCAGTCCAATCTACTTTACAACACCGTGTTTTGTCGAAGAGATATAGGGACACATGGAAGCTATCTCGCGACCTCGATTTCAGCGGAATATTTTCTGTCAGACGTAGTCAATCAGAGGCTGTACGCATAATCGAAGACGTTTTTATTCAGCACCAAGGACCAGAGATCGACCGGTTTGTTTTGTCTCTCGACCATACCGGCGTGGAGGATAAGATACTTTCGTGGGTCGATACGTGTCTTCGTAAAAACATCAAAGAGCTTGTGTTGGATTTCTCAAGCTCCAAGAAGGTTATGGAGATCCCACTCAATTTCTCATCCAGCGAGACACTTACTGTTTTGAAGCTGCAATGGTGTAGATTTGAAATACCGAATAATCTTCCAAAAGGTCTAAGGCTCTTGAAGACACTTTCGCTCATGCGGACTGAGGTAACGAATGAAATGATAGATTCAATTTTCAACAACTGCATCCACCTTGAGTCGCTTGAACTAGTCAAATGCCAAATGTCTGGGAATGGGATACTGACCATATACGCTCATGATCATAAGAAGTTCAAGTCGTTGGTCGTGTCTTGTATGCCAAATCTTTTGAGCATCTTTTTGGATGCACCCACTCTTGAATGTTACAAGTATGATGGATATGCCAAGACCATCAACGTTTTAAAGGTTAATGCACTTGAAGAGGCAGAATTTCATTATAGTCGAAGTAAAAGAAGGCATTCTTCTGACACGGTGGTTGATAGTATGCTAGCCTGTTCAGCAGTTCATGCCCTCACAACGACAAACATCTTGCTTGAGGCAATCACGTATAGATACTTAAAAGGGAAACTGGAAAAGCCGCTTTTCAAGTTTGAGAACTTGAGAGAATTCAAGATTTTCTTTAAGGCTCCAACATTTTGCACTCTCTTTGATATCGCTGAATTCCTTAAAGAGTGCCCTAAACTCGAACAGTTTATCATTGATATTCAGAATTTCACATTTGAACCTCAAATGTATTTTTGGGAGATACATCATAAGGCGCAGATTCAGAATACTTCGAATAACAACTACCTTTTGAAGTGTCTCACGGACGTGAAGATCATTGGCTATAAAGGCCACTGGCATGAGCTTGATATAGTAGAGTTCTTTGTCAAGAACGCACCATCTCTAAAGAGGTTGGAGTTACAGATGCCTAAGAACGCGAAGAACGATGCTCACACCCCAGATGTTGCAAGGATAAAGCTCATAAAAACCATATTTTCAGGGGTTAAAGTTACCGAAGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box FBD

Protein Analysis

447

Amino Acids

51.88

Weight (kDa)

8.6

Isoelectric Point (pI)

45.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_At1g61320_AtMIF1 PF23622 77 - 421 1.8e-24 At1g61320/AtMIF1, LRR domain
LRR_At5g56370 PF24758 101 - 290 6.3e-14 FBD-associated F-box protein At5g56370, LRR repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016662)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G61320 AT1G61330
malus_domestica MD10G1303300.v1.1
prunus_persica Prupe.4G036700_v2.0.a1
pyrus_communis pycom10g25580
rosa_laevigata RLG00000019732
rosa_roxburghii Rroxscaffold_1G00069650 Rroxscaffold_1G00069660
rosa_rugosa Rorug04G0421300
rosa_samantha Rh2BG409000 Rh5BG048300 Rh5BG427600 Rh5DG047400 Rh5DG047500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 150, 1259
AciI CCGC 3 cut(s) 165, 499, 917
AclI AACGTT 1 cut(s) 735
AclWI GGATC 1 cut(s) 373
AcsI RAATTY 4 cut(s) 767, 944, 998, 1048
AfaI GTAC 1 cut(s) 204
AflIII ACRYGT 1 cut(s) 314
AgeI ACCGGT 1 cut(s) 252
AhlI ACTAGT 1 cut(s) 568
AjiI CACGTC 1 cut(s) 1150
AleI CACNNNNGTG 1 cut(s) 1148
AluBI AGCT 6 cut(s) 142, 340, 360, 424, 1186, 1300
AluI AGCT 6 cut(s) 142, 340, 360, 424, 1186, 1300
Alw26I GTCTC 3 cut(s) 270, 398, 1145
AlwI GGATC 1 cut(s) 373
AlwNI CAGNNNCTG 1 cut(s) 200
AoxI GGCC 1 cut(s) 1171
ApeKI GCWGC 1 cut(s) 424
ApoI RAATTY 4 cut(s) 767, 944, 998, 1048
AsiGI ACCGGT 1 cut(s) 252
Asp700I GAANNNNTTC 2 cut(s) 172, 796
AspLEI GCGC 1 cut(s) 1099
AspS9I GGNCC 1 cut(s) 239
AsuII TTCGAA 1 cut(s) 1115
AsuNHI GCTAGC 1 cut(s) 826
AvaII GGWCC 1 cut(s) 239
BaeGI GKGCMC 1 cut(s) 1016
BaeI ACNNNNGTAYC 2 cut(s) 287, 320
BbsI GAAGAC 3 cut(s) 222, 311, 488
BbvI GCAGC 1 cut(s) 411
BccI CCATC 3 cut(s) 707, 737, 1228
BciVI GTATCC 1 cut(s) 591
BclI TGATCA 1 cut(s) 619
BcoDI GTCTC 3 cut(s) 270, 398, 1145
BcuI ACTAGT 1 cut(s) 568
BfaI CTAG 2 cut(s) 569, 827
BfuI GTATCC 1 cut(s) 591
BisI GCNGC 2 cut(s) 425, 917
BlsI GCNGC 2 cut(s) 426, 918
Bme18I GGWCC 1 cut(s) 239
BmgBI CACGTC 1 cut(s) 1150
BmgT120I GGNCC 1 cut(s) 239
BmiI GGNNCC 1 cut(s) 966
BmsI GCATC 6 cut(s) 75, 555, 673, 681, 1236, 1258
BmtI GCTAGC 1 cut(s) 830
BpiI GAAGAC 3 cut(s) 222, 311, 488
Bpu14I TTCGAA 1 cut(s) 1115
BpuEI CTTGAG 4 cut(s) 340, 575, 893, 958
BsaAI YACGTR 2 cut(s) 315, 885
BsaJI CCNNGG 1 cut(s) 235
BsaWI WCCGGW 1 cut(s) 252
Bse118I RCCGGY 2 cut(s) 252, 278
Bse1I ACTGG 3 cut(s) 83, 912, 1181
Bse3DI GCAATG 1 cut(s) 434
BseDI CCNNGG 1 cut(s) 235
BseGI GGATG 3 cut(s) 395, 546, 688
BseMI GCAATG 1 cut(s) 434
BseMII CTCAG 1 cut(s) 495
BseNI ACTGG 3 cut(s) 83, 912, 1181
BseSI GKGCMC 1 cut(s) 1016
BseXI GCAGC 1 cut(s) 411
Bsh1236I CGCG 2 cut(s) 150, 1259
Bsh1285I CGRYCG 1 cut(s) 253
BshFI GGCC 1 cut(s) 1173
BshTI ACCGGT 1 cut(s) 252
BsiEI CGRYCG 1 cut(s) 253
BsiSI CCGG 2 cut(s) 253, 279
BslFI GGGAC 1 cut(s) 143
BsmAI GTCTC 3 cut(s) 270, 398, 1145
BsmFI GGGAC 1 cut(s) 143
BsmI GAATGC 1 cut(s) 796
BsnI GGCC 1 cut(s) 1173
Bsp119I TTCGAA 1 cut(s) 1115
Bsp1286I GDGCHC 1 cut(s) 1016
Bsp143I GATC 4 cut(s) 246, 378, 619, 1155
Bsp68I TCGCGA 1 cut(s) 150
BspACI CCGC 3 cut(s) 165, 499, 917
BspANI GGCC 1 cut(s) 1173
BspCNI CTCAG 1 cut(s) 496
BspFNI CGCG 2 cut(s) 150, 1259
BspHI TCATGA 1 cut(s) 616
BspLI GGNNCC 1 cut(s) 966
BspOI GCTAGC 1 cut(s) 830
BspPI GGATC 1 cut(s) 373
BspT104I TTCGAA 1 cut(s) 1115
BsrDI GCAATG 1 cut(s) 434
BsrFI RCCGGY 2 cut(s) 252, 278
BsrI ACTGG 3 cut(s) 83, 912, 1181
BssAI RCCGGY 2 cut(s) 252, 278
BssECI CCNNGG 1 cut(s) 235
BssMI GATC 4 cut(s) 246, 378, 619, 1155
BssT1I CCWWGG 1 cut(s) 235
Bst4CI ACNGT 4 cut(s) 107, 415, 811, 1029
Bst6I CTCTTC 2 cut(s) 113, 753
BstAPI GCANNNNNTGC 1 cut(s) 845
BstBAI YACGTR 2 cut(s) 315, 885
BstBI TTCGAA 1 cut(s) 1115
BstC8I GCNNGC 1 cut(s) 828
BstDEI CTNAG 3 cut(s) 470, 504, 1251
BstF5I GGATG 3 cut(s) 395, 546, 688
BstFNI CGCG 2 cut(s) 150, 1259
BstHHI GCGC 1 cut(s) 1099
BstKTI GATC 4 cut(s) 249, 381, 622, 1158
BstMAI GTCTC 3 cut(s) 270, 398, 1145
BstMBI GATC 4 cut(s) 246, 378, 619, 1155
BstMCI CGRYCG 1 cut(s) 253
BstMWI GCNNNNNNNGC 3 cut(s) 836, 845, 1170
BstSLI GKGCMC 1 cut(s) 1016
BstUI CGCG 2 cut(s) 150, 1259
BstV1I GCAGC 1 cut(s) 411
BstV2I GAAGAC 3 cut(s) 222, 311, 488
BstX2I RGATCY 1 cut(s) 378
BstXI CCANNNNNNTGG 1 cut(s) 587
BstYI RGATCY 1 cut(s) 378
BsuI GTATCC 1 cut(s) 591
BsuRI GGCC 1 cut(s) 1173
BtrI CACGTC 1 cut(s) 1150
BtsCI GGATG 3 cut(s) 395, 546, 688
BtsIMutI CAGTG 1 cut(s) 1174
BtuMI TCGCGA 1 cut(s) 150
Cac8I GCNNGC 1 cut(s) 828
CaiI CAGNNNCTG 1 cut(s) 200
CciI TCATGA 1 cut(s) 616
CfoI GCGC 1 cut(s) 1099
Cfr10I RCCGGY 2 cut(s) 252, 278
Cfr13I GGNCC 1 cut(s) 239
Csp6I GTAC 1 cut(s) 203
CspAI ACCGGT 1 cut(s) 252
CviAII CATG 5 cut(s) 135, 496, 617, 845, 1181
CviQI GTAC 1 cut(s) 203
DdeI CTNAG 3 cut(s) 470, 504, 1251
DpnI GATC 4 cut(s) 248, 380, 621, 1157
DpnII GATC 4 cut(s) 246, 378, 619, 1155
DraI TTTAAA 1 cut(s) 741
Eam1104I CTCTTC 2 cut(s) 113, 753
EarI CTCTTC 2 cut(s) 113, 753
Eco130I CCWWGG 1 cut(s) 235
Eco32I GATATC 1 cut(s) 991
Eco47I GGWCC 1 cut(s) 239
EcoRI GAATTC 2 cut(s) 944, 998
EcoRV GATATC 1 cut(s) 991
EcoT14I CCWWGG 1 cut(s) 235
ErhI CCWWGG 1 cut(s) 235
FaeI CATG 5 cut(s) 138, 499, 620, 848, 1184
FalI AAGNNNNNCTT 4 cut(s) 784, 816, 941, 973
FaqI GGGAC 1 cut(s) 143
FatI CATG 5 cut(s) 134, 495, 616, 844, 1180
FbaI TGATCA 1 cut(s) 619
Fnu4HI GCNGC 2 cut(s) 425, 917
FokI GGATG 3 cut(s) 382, 533, 695
Fsp4HI GCNGC 2 cut(s) 425, 917
FspBI CTAG 2 cut(s) 569, 827
GlaI GCGC 1 cut(s) 1098
GluI GCNGC 2 cut(s) 425, 917
HaeIII GGCC 1 cut(s) 1173
HapII CCGG 2 cut(s) 253, 279
HhaI GCGC 1 cut(s) 1099
Hin1II CATG 5 cut(s) 138, 499, 620, 848, 1184
Hin6I GCGC 1 cut(s) 1097
HinP1I GCGC 1 cut(s) 1097
HinfI GANTC 3 cut(s) 527, 557, 1102
HpaII CCGG 2 cut(s) 253, 279
Hpy188I TCNGA 7 cut(s) 7, 49, 182, 196, 805, 1047, 1107
Hpy188III TCNNGA 7 cut(s) 149, 269, 478, 617, 695, 949, 1210
HpyAV CCTTC 2 cut(s) 361, 786
HpyCH4III ACNGT 4 cut(s) 107, 415, 811, 1029
HpyCH4IV ACGT 6 cut(s) 184, 219, 314, 735, 884, 1149
HpyCH4V TGCA 7 cut(s) 66, 427, 546, 686, 752, 978, 1289
HpyF10VI GCNNNNNNNGC 3 cut(s) 836, 845, 1170
HpyF3I CTNAG 3 cut(s) 470, 504, 1251
HpySE526I ACGT 6 cut(s) 184, 219, 314, 735, 884, 1149
Hsp92II CATG 5 cut(s) 138, 499, 620, 848, 1184
HspAI GCGC 1 cut(s) 1097
Ksp22I TGATCA 1 cut(s) 619
Kzo9I GATC 4 cut(s) 246, 378, 619, 1155
LmnI GCTCC 2 cut(s) 365, 970
Lsp1109I GCAGC 1 cut(s) 411
LweI GCATC 6 cut(s) 75, 555, 673, 681, 1236, 1258
MaeI CTAG 2 cut(s) 569, 827
MaeII ACGT 6 cut(s) 184, 219, 314, 735, 884, 1149
MaeIII GTNAC 4 cut(s) 508, 701, 1239, 1330
MalI GATC 4 cut(s) 248, 380, 621, 1157
MboI GATC 4 cut(s) 246, 378, 619, 1155
MflI RGATCY 1 cut(s) 378
MhlI GDGCHC 1 cut(s) 1016
MluCI AATT 6 cut(s) 388, 531, 767, 944, 998, 1048
MlyI GAGTC 1 cut(s) 566
MmeI TCCRAC 3 cut(s) 327, 992, 1215
MnlI CCTC 9 cut(s) 164, 190, 280, 499, 754, 860, 868, 1074, 1224
MroXI GAANNNNTTC 2 cut(s) 172, 796
MseI TTAA 7 cut(s) 740, 747, 896, 960, 1005, 1326, 1342
MslI CAYNNNNRTG 2 cut(s) 1148, 1179
MspA1I CMGCKG 1 cut(s) 165
MspI CCGG 2 cut(s) 253, 279
Mva1269I GAATGC 1 cut(s) 796
MvnI CGCG 2 cut(s) 150, 1259
MwoI GCNNNNNNNGC 3 cut(s) 836, 845, 1170
NdeII GATC 4 cut(s) 246, 378, 619, 1155
NheI GCTAGC 1 cut(s) 826
NlaIII CATG 5 cut(s) 138, 499, 620, 848, 1184
NlaIV GGNNCC 1 cut(s) 966
NruI TCGCGA 1 cut(s) 150
NspV TTCGAA 1 cut(s) 1115
OliI CACNNNNGTG 1 cut(s) 1148
PagI TCATGA 1 cut(s) 616
PcsI WCGNNNNNNNCGW 1 cut(s) 1263
PctI GAATGC 1 cut(s) 796
PdmI GAANNNNTTC 2 cut(s) 172, 796
PfeI GAWTC 2 cut(s) 527, 1102
PflFI GACNNNGTC 1 cut(s) 186
PinAI ACCGGT 1 cut(s) 252
PkrI GCNGC 2 cut(s) 426, 918
PleI GAGTC 1 cut(s) 565
PpsI GAGTC 1 cut(s) 565
Ppu21I YACGTR 2 cut(s) 315, 885
Psp1406I AACGTT 1 cut(s) 735
PspN4I GGNNCC 1 cut(s) 966
PspPI GGNCC 1 cut(s) 239
PstNI CAGNNNCTG 1 cut(s) 200
PsuI RGATCY 1 cut(s) 378
PsyI GACNNNGTC 1 cut(s) 186
RruI TCGCGA 1 cut(s) 150
RsaI GTAC 1 cut(s) 204
RsaNI GTAC 1 cut(s) 203
RseI CAYNNNNRTG 2 cut(s) 1148, 1179
SaqAI TTAA 7 cut(s) 740, 747, 896, 960, 1005, 1326, 1342
SatI GCNGC 2 cut(s) 425, 917
Sau3AI GATC 4 cut(s) 246, 378, 619, 1155
Sau96I GGNCC 1 cut(s) 239
SchI GAGTC 1 cut(s) 566
SduI GDGCHC 1 cut(s) 1016
SfaNI GCATC 6 cut(s) 75, 555, 673, 681, 1236, 1258
SfuI TTCGAA 1 cut(s) 1115
SinI GGWCC 1 cut(s) 239
SmiMI CAYNNNNRTG 2 cut(s) 1148, 1179
SmlI CTYRAG 4 cut(s) 355, 554, 872, 937
SmoI CTYRAG 4 cut(s) 355, 554, 872, 937
SpeI ACTAGT 1 cut(s) 568
Sse9I AATT 6 cut(s) 388, 531, 767, 944, 998, 1048
SsiI CCGC 3 cut(s) 165, 499, 917
SspI AATATT 1 cut(s) 171
SspMI CTAG 2 cut(s) 569, 827
StyI CCWWGG 1 cut(s) 235
TaaI ACNGT 4 cut(s) 107, 415, 811, 1029
TaiI ACGT 6 cut(s) 187, 222, 317, 738, 887, 1152
TaqI TCGA 9 cut(s) 116, 156, 213, 249, 270, 309, 781, 1023, 1115
TasI AATT 6 cut(s) 388, 531, 767, 944, 998, 1048
TauI GCSGC 1 cut(s) 919
TfiI GAWTC 2 cut(s) 527, 1102
Tru1I TTAA 7 cut(s) 740, 747, 896, 960, 1005, 1326, 1342
Tru9I TTAA 7 cut(s) 740, 747, 896, 960, 1005, 1326, 1342
TscAI CASTG 1 cut(s) 1181
TseI GCWGC 1 cut(s) 424
TspDTI ATGAA 4 cut(s) 41, 531, 761, 833
TspGWI ACGGA 1 cut(s) 1160
TspRI CASTG 1 cut(s) 1181
Tth111I GACNNNGTC 1 cut(s) 186
VpaK11BI GGWCC 1 cut(s) 239
XapI RAATTY 4 cut(s) 767, 944, 998, 1048
XcmI CCANNNNNNNNNTGG 1 cut(s) 370
XmnI GAANNNNTTC 2 cut(s) 172, 796
XspI CTAG 2 cut(s) 569, 827
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.