AT1G64680

Domain of unknown function (DUF4033)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0016208
Physical Location & Seq
Forward (+)
24035941 .. 24037429
1489 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G64680.1

Sequence Viewer

Length: 753 bp
ATGGCGGCTATTGCTAGTCTGCAAGCAGTTAATCTCACATTTAGGCGACGTAGCACTCGATGTGGAATTGCTGAGCCGAGCGGAGAGCCAGCTCCGATGGGGCTGAAGACTAGATACGAGGATGGGCTGGTGGAGAGAGTGTTCATGGGTCTCTTCGCGAGGAAGATGGACAAGTTCGGGTCGAAGAAGAAGAAGGACACGAAGGAGAAGGGTTTTTGGGAGTACGATTACGAGAGCTTCGTGGAGGTGTCAAAGAGAGTGATGCAGGGACGGTCCAGAGTGCAGCAGCAAGAGGCCGTGAGGGAGGTTCTTCTCTCTATGCTGCCTCCTGGTGCTCCTGAACAGTTTAGGAAATTGTTCCCACCAACGAAATGGGCTGCAGAGTTCAATGCCGCTCTTACAGTGCCTTTCTTTCACTGGTTGGTTGGTCCATCTCAGGTCATAGAAGTGGAAGTGAATGGTGTGAAACAGAGAAGTGGAGTTCGTATCAAGAAATGCAGGTATCTGGAGAACAGTGGGTGTGTAGGAATGTGTGTGAATATGTGCAAGATTCCAACCCAAGATTTCTTCACCAATGAGTTTGGCCTCCCACTCACCATGAACCCAAATTATGAAGACATGAGCTGCGAGATGATATACGGGCAAGCACCTCCGGCCTTTGAGGAGGATGTAGCCACCAAGCAACCTTGTCTAGCAGATATATGTTCTATGTCGAATCCAAGCTCCCCAATCTGCCCTAAACTAGAGGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4033

Protein Analysis

250

Amino Acids

28.16

Weight (kDa)

8.52

Isoelectric Point (pI)

51.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
D27-like_C PF13225 140 - 222 1.7e-38 Beta-carotene isomerase D27-like, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016208)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G64680 AT1G64680
fragaria_vesca FvH4_5g10820
malus_domestica MD14G1137300.v1.1
prunus_persica Prupe.5G131200_v2.0.a1
pyrus_communis pycom14g11240
rosa_chinensis RchiOBHm_Chr7g0187341
rosa_laevigata RLG00000004793
rosa_roxburghii Rroxscaffold_3G00267590
rosa_rugosa Rorug06G0480700
rosa_samantha Rh7AG085200 Rh7BG077400 Rh7CG086200 Rh7DG087300
rosa_wichuraiana Rw7G007490

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 489
AccBSI CCGCTC 2 cut(s) 81, 395
AccII CGCG 1 cut(s) 158
AciI CCGC 3 cut(s) 5, 81, 393
AcuI CTGAAG 1 cut(s) 125
AfaI GTAC 1 cut(s) 224
AgsI TTSAA 1 cut(s) 388
AjnI CCWGG 1 cut(s) 328
AloI GAACNNNNNNTCC 2 cut(s) 125, 157
AluBI AGCT 4 cut(s) 92, 237, 624, 723
AluI AGCT 4 cut(s) 92, 237, 624, 723
Alw21I GWGCWC 1 cut(s) 337
Alw26I GTCTC 1 cut(s) 155
AoxI GGCC 3 cut(s) 294, 583, 654
ApeKI GCWGC 5 cut(s) 283, 286, 322, 377, 624
Asp700I GAANNNNTTC 1 cut(s) 356
AspS9I GGNCC 2 cut(s) 273, 428
AsuHPI GGTGA 2 cut(s) 562, 586
AvaII GGWCC 2 cut(s) 273, 428
BarI GAAGNNNNNNTAC 2 cut(s) 98, 130
BbsI GAAGAC 2 cut(s) 113, 621
Bbv12I GWGCWC 1 cut(s) 337
BbvI GCAGC 5 cut(s) 295, 298, 309, 364, 611
BccI CCATC 4 cut(s) 91, 116, 160, 439
BceAI ACGGC 1 cut(s) 281
BciT130I CCWGG 1 cut(s) 330
BcoDI GTCTC 1 cut(s) 155
BfaI CTAG 4 cut(s) 15, 111, 692, 743
BfmI CTRYAG 1 cut(s) 378
BfuAI ACCTGC 1 cut(s) 489
BisI GCNGC 7 cut(s) 6, 284, 287, 323, 378, 393, 625
BlpI GCTNAGC 1 cut(s) 72
BlsI GCNGC 7 cut(s) 7, 285, 288, 324, 379, 394, 626
Bme1390I CCNGG 1 cut(s) 330
Bme18I GGWCC 2 cut(s) 273, 428
BmgT120I GGNCC 2 cut(s) 273, 428
BmrFI CCNGG 1 cut(s) 330
BmsI GCATC 1 cut(s) 252
BpiI GAAGAC 2 cut(s) 113, 621
BpmI CTGGAG 1 cut(s) 527
Bpu1102I GCTNAGC 1 cut(s) 72
BsaI GGTCTC 1 cut(s) 155
BsaXI ACNNNNNCTCC 2 cut(s) 125, 155
Bse1I ACTGG 1 cut(s) 422
BseBI CCWGG 1 cut(s) 330
BseGI GGATG 2 cut(s) 127, 673
BseMII CTCAG 2 cut(s) 63, 449
BseNI ACTGG 1 cut(s) 422
BseRI GAGGAG 1 cut(s) 677
BseXI GCAGC 5 cut(s) 295, 298, 309, 364, 611
BsgI GTGCAG 1 cut(s) 302
Bsh1236I CGCG 1 cut(s) 158
BshFI GGCC 3 cut(s) 296, 585, 656
BsiHKAI GWGCWC 1 cut(s) 337
BsiSI CCGG 1 cut(s) 653
BslFI GGGAC 1 cut(s) 282
BsmAI GTCTC 1 cut(s) 155
BsmFI GGGAC 1 cut(s) 282
BsnI GGCC 3 cut(s) 296, 585, 656
Bso31I GGTCTC 1 cut(s) 155
Bsp1286I GDGCHC 1 cut(s) 337
Bsp1720I GCTNAGC 1 cut(s) 72
Bsp68I TCGCGA 1 cut(s) 158
BspACI CCGC 3 cut(s) 5, 81, 393
BspANI GGCC 3 cut(s) 296, 585, 656
BspCNI CTCAG 2 cut(s) 64, 448
BspFNI CGCG 1 cut(s) 158
BspMAI CTGCAG 1 cut(s) 382
BspMI ACCTGC 1 cut(s) 489
BspTNI GGTCTC 1 cut(s) 155
BsrBI CCGCTC 2 cut(s) 81, 395
BsrI ACTGG 1 cut(s) 422
Bst2UI CCWGG 1 cut(s) 330
Bst4CI ACNGT 4 cut(s) 273, 345, 403, 515
Bst6I CTCTTC 1 cut(s) 158
BstC8I GCNNGC 3 cut(s) 24, 90, 645
BstDEI CTNAG 2 cut(s) 72, 435
BstF5I GGATG 2 cut(s) 127, 673
BstFNI CGCG 1 cut(s) 158
BstMAI GTCTC 1 cut(s) 155
BstMWI GCNNNNNNNGC 2 cut(s) 11, 653
BstNI CCWGG 1 cut(s) 330
BstSCI CCNGG 1 cut(s) 328
BstSFI CTRYAG 1 cut(s) 378
BstUI CGCG 1 cut(s) 158
BstV1I GCAGC 5 cut(s) 295, 298, 309, 364, 611
BstV2I GAAGAC 2 cut(s) 113, 621
BstXI CCANNNNNNTGG 1 cut(s) 372
BsuRI GGCC 3 cut(s) 296, 585, 656
BtsCI GGATG 2 cut(s) 127, 673
BtsIMutI CAGTG 3 cut(s) 408, 415, 520
BtuMI TCGCGA 1 cut(s) 158
BveI ACCTGC 1 cut(s) 489
Cac8I GCNNGC 3 cut(s) 24, 90, 645
Cfr13I GGNCC 2 cut(s) 273, 428
Csp6I GTAC 1 cut(s) 223
CviAII CATG 4 cut(s) 145, 598, 619, 750
CviQI GTAC 1 cut(s) 223
DdeI CTNAG 2 cut(s) 72, 435
Eam1104I CTCTTC 1 cut(s) 158
EarI CTCTTC 1 cut(s) 158
Eco31I GGTCTC 1 cut(s) 155
Eco47I GGWCC 2 cut(s) 273, 428
Eco57I CTGAAG 1 cut(s) 125
EcoRII CCWGG 1 cut(s) 328
FaeI CATG 4 cut(s) 148, 601, 622, 753
FaqI GGGAC 1 cut(s) 282
FatI CATG 4 cut(s) 144, 597, 618, 749
Fnu4HI GCNGC 7 cut(s) 6, 284, 287, 323, 378, 393, 625
FokI GGATG 2 cut(s) 134, 680
Fsp4HI GCNGC 7 cut(s) 6, 284, 287, 323, 378, 393, 625
FspBI CTAG 4 cut(s) 15, 111, 692, 743
GluI GCNGC 7 cut(s) 6, 284, 287, 323, 378, 393, 625
GsuI CTGGAG 1 cut(s) 527
HaeIII GGCC 3 cut(s) 296, 585, 656
HapII CCGG 1 cut(s) 653
Hin1II CATG 4 cut(s) 148, 601, 622, 753
HinfI GANTC 2 cut(s) 550, 715
HpaII CCGG 1 cut(s) 653
HphI GGTGA 2 cut(s) 562, 586
Hpy188I TCNGA 1 cut(s) 96
Hpy188III TCNNGA 5 cut(s) 157, 276, 338, 490, 506
Hpy99I CGWCG 1 cut(s) 51
HpyAV CCTTC 3 cut(s) 187, 196, 202
HpyCH4III ACNGT 4 cut(s) 273, 345, 403, 515
HpyCH4IV ACGT 1 cut(s) 49
HpyCH4V TGCA 6 cut(s) 22, 265, 283, 380, 498, 546
HpyF10VI GCNNNNNNNGC 2 cut(s) 11, 653
HpyF3I CTNAG 2 cut(s) 72, 435
HpySE526I ACGT 1 cut(s) 49
Hsp92II CATG 4 cut(s) 148, 601, 622, 753
LmnI GCTCC 3 cut(s) 97, 340, 728
Lsp1109I GCAGC 5 cut(s) 295, 298, 309, 364, 611
LweI GCATC 1 cut(s) 252
MaeI CTAG 4 cut(s) 15, 111, 692, 743
MaeII ACGT 1 cut(s) 49
MbiI CCGCTC 2 cut(s) 81, 395
MboII GAAGA 9 cut(s) 118, 145, 175, 196, 199, 202, 302, 559, 626
MhlI GDGCHC 1 cut(s) 337
MluCI AATT 3 cut(s) 66, 353, 607
MmeI TCCRAC 1 cut(s) 578
MroXI GAANNNNTTC 1 cut(s) 356
MseI TTAA 1 cut(s) 30
MslI CAYNNNNRTG 1 cut(s) 446
MspI CCGG 1 cut(s) 653
MspR9I CCNGG 1 cut(s) 330
MvaI CCWGG 1 cut(s) 330
MvnI CGCG 1 cut(s) 158
MwoI GCNNNNNNNGC 2 cut(s) 11, 653
NlaIII CATG 4 cut(s) 148, 601, 622, 753
NmeAIII GCCGAG 1 cut(s) 102
NruI TCGCGA 1 cut(s) 158
PcsI WCGNNNNNNNCGW 2 cut(s) 55, 237
PdmI GAANNNNTTC 1 cut(s) 356
PfeI GAWTC 2 cut(s) 550, 715
PkrI GCNGC 7 cut(s) 7, 285, 288, 324, 379, 394, 626
Psp6I CCWGG 1 cut(s) 328
PspGI CCWGG 1 cut(s) 328
PspPI GGNCC 2 cut(s) 273, 428
PstI CTGCAG 1 cut(s) 382
RruI TCGCGA 1 cut(s) 158
RsaI GTAC 1 cut(s) 224
RsaNI GTAC 1 cut(s) 223
RseI CAYNNNNRTG 1 cut(s) 446
SaqAI TTAA 1 cut(s) 30
SatI GCNGC 7 cut(s) 6, 284, 287, 323, 378, 393, 625
Sau96I GGNCC 2 cut(s) 273, 428
ScrFI CCNGG 1 cut(s) 330
SduI GDGCHC 1 cut(s) 337
SfaNI GCATC 1 cut(s) 252
SfcI CTRYAG 1 cut(s) 378
SinI GGWCC 2 cut(s) 273, 428
SmiMI CAYNNNNRTG 1 cut(s) 446
Sse9I AATT 3 cut(s) 66, 353, 607
SsiI CCGC 3 cut(s) 5, 81, 393
SspMI CTAG 4 cut(s) 15, 111, 692, 743
StyD4I CCNGG 1 cut(s) 328
TaaI ACNGT 4 cut(s) 273, 345, 403, 515
TaiI ACGT 1 cut(s) 52
TaqI TCGA 3 cut(s) 58, 182, 713
TasI AATT 3 cut(s) 66, 353, 607
TauI GCSGC 2 cut(s) 8, 395
TfiI GAWTC 2 cut(s) 550, 715
Tru1I TTAA 1 cut(s) 30
Tru9I TTAA 1 cut(s) 30
TscAI CASTG 3 cut(s) 408, 422, 520
TseI GCWGC 5 cut(s) 283, 286, 322, 377, 624
TspDTI ATGAA 3 cut(s) 133, 614, 627
TspRI CASTG 3 cut(s) 408, 422, 520
VpaK11BI GGWCC 2 cut(s) 273, 428
XcmI CCANNNNNNNNNTGG 1 cut(s) 369
XmnI GAANNNNTTC 1 cut(s) 356
XspI CTAG 4 cut(s) 15, 111, 692, 743
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.