MD14G1137300.v1.1

Beta-carotene isomerase D27

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0016208
Physical Location & Seq
Forward (+)
22418945 .. 22421258
2314 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1137300.v1.1.491

Sequence Viewer

Length: 789 bp
ATGGTGGTTCTAAGCTTTCAAACTGTCCAATTTACAACACCTAAACACTTATCGTTATGTAAACCGAGTGTTTCTGGTTGCTCCGTACGATGTGGTATTGCAGAACCATCGGGAGAGCCGGCTCCAATGGGGCAAAAAACCAAGTACAACGATGGGTTTTTCGAGAGGGGATTCATGACACTGTTTGCTCGGAAAATGATGAAGTTTGCAGCTCCCGCGAAACCGAGAACTGAGACGAAGGAGAAGGCGTGGTGGGAATATGACTACGAGAGCTTTGTGGATGTGTCCAAGAGAGTGATGCAGGGGAGGTCTCGGAGTCAGCAGCAGAAGGTGGTTCGAGAAGTGCTTTTGTCTATGCTACCTCCAGGTGCGCCAGAGCAGTTTAAGAAACTATTTCCACCAACAAAGTGGGCAGCTGAGTTCAATGCAGCATTGACAGTGCCTTTCTTCCACTGGTTGGTTGGGCCTTCTGAGGTTGTAGAAGTTGAGATAGATGGGGTGAAGCAAAGAAGTGGAGTTCATATAAAGAAATGCAGGTACCTGGAGAACAGCGGGTGTGTTGGAATGTGTGTGAATATGTGCAAGTTTCCTACACAAGATTTCTTCACCAATGAATTCGGACTTCCCTTAACCATGATTCCAAATTTCGAAGATATGAGCTGTGAAATGGTGTATGGCCAAGTTCCACCACCATTTGAAGAGGATCCAGTCTCTAAACAACCATGTTTTACTGATATATGTTCCATTGCAAATCCCAGCTCCAGTGTTTGTCCAAAACTACAAAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4033

Protein Analysis

263

Amino Acids

29.53

Weight (kDa)

8.24

Isoelectric Point (pI)

46.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
D27-like_C PF13225 152 - 234 3.5e-38 Beta-carotene isomerase D27-like, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016208)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G64680 AT1G64680
fragaria_vesca FvH4_5g10820
malus_domestica MD14G1137300.v1.1
prunus_persica Prupe.5G131200_v2.0.a1
pyrus_communis pycom14g11240
rosa_chinensis RchiOBHm_Chr7g0187341
rosa_laevigata RLG00000004793
rosa_roxburghii Rroxscaffold_3G00267590
rosa_rugosa Rorug06G0480700
rosa_samantha Rh7AG085200 Rh7BG077400 Rh7CG086200 Rh7DG087300
rosa_wichuraiana Rw7G007490

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 525
Acc65I GGTACC 1 cut(s) 537
AccB1I GGYRCC 1 cut(s) 537
AccB7I CCANNNNNTGG 1 cut(s) 457
AccII CGCG 1 cut(s) 218
AciI CCGC 2 cut(s) 216, 552
AclWI GGATC 2 cut(s) 698, 711
AcoI YGGCCR 1 cut(s) 676
AcsI RAATTY 2 cut(s) 614, 643
AfaI GTAC 3 cut(s) 87, 146, 539
AfiI CCNNNNNNNGG 1 cut(s) 457
AgsI TTSAA 3 cut(s) 20, 424, 698
AjnI CCWGG 2 cut(s) 364, 540
AluBI AGCT 6 cut(s) 15, 212, 273, 416, 660, 759
AluI AGCT 6 cut(s) 15, 212, 273, 416, 660, 759
Alw26I GTCTC 3 cut(s) 227, 315, 715
AlwI GGATC 2 cut(s) 698, 711
AoxI GGCC 2 cut(s) 464, 676
ApeKI GCWGC 4 cut(s) 209, 322, 413, 428
ApoI RAATTY 2 cut(s) 614, 643
Asp718I GGTACC 1 cut(s) 537
AspLEI GCGC 1 cut(s) 373
AspS9I GGNCC 1 cut(s) 464
AsuHPI GGTGA 2 cut(s) 511, 598
AsuII TTCGAA 1 cut(s) 648
BalI TGGCCA 1 cut(s) 678
BamHI GGATCC 1 cut(s) 703
BanI GGYRCC 1 cut(s) 537
BbvI GCAGC 4 cut(s) 221, 334, 425, 440
BccI CCATC 3 cut(s) 115, 146, 488
BcgI CGANNNNNNTGC 2 cut(s) 90, 124
BciT130I CCWGG 2 cut(s) 366, 542
BcoDI GTCTC 3 cut(s) 227, 315, 715
BfuAI ACCTGC 1 cut(s) 525
BisI GCNGC 4 cut(s) 210, 323, 414, 429
BlsI GCNGC 4 cut(s) 211, 324, 415, 430
Bme1390I CCNGG 2 cut(s) 366, 542
BmgT120I GGNCC 1 cut(s) 464
BmiI GGNNCC 3 cut(s) 123, 539, 705
BmrFI CCNGG 2 cut(s) 366, 542
BmsI GCATC 1 cut(s) 288
BpmI CTGGAG 3 cut(s) 348, 563, 745
Bpu14I TTCGAA 1 cut(s) 648
BsaI GGTCTC 1 cut(s) 315
BsaXI ACNNNNNCTCC 2 cut(s) 233, 263
Bsc4I CCNNNNNNNGG 1 cut(s) 457
Bse118I RCCGGY 1 cut(s) 118
Bse1I ACTGG 3 cut(s) 458, 707, 762
Bse3DI GCAATG 1 cut(s) 744
BseBI CCWGG 2 cut(s) 366, 542
BseGI GGATG 1 cut(s) 286
BseLI CCNNNNNNNGG 1 cut(s) 457
BseMI GCAATG 1 cut(s) 744
BseMII CTCAG 3 cut(s) 222, 408, 462
BseNI ACTGG 3 cut(s) 458, 707, 762
BseXI GCAGC 4 cut(s) 221, 334, 425, 440
BseYI CCCAGC 1 cut(s) 755
Bsh1236I CGCG 1 cut(s) 218
BshFI GGCC 2 cut(s) 466, 678
BshNI GGYRCC 1 cut(s) 537
BsiSI CCGG 1 cut(s) 119
BsiWI CGTACG 1 cut(s) 85
BslI CCNNNNNNNGG 1 cut(s) 457
BsmAI GTCTC 3 cut(s) 227, 315, 715
BsmBI CGTCTC 1 cut(s) 227
BsnI GGCC 2 cut(s) 466, 678
Bso31I GGTCTC 1 cut(s) 315
Bsp119I TTCGAA 1 cut(s) 648
Bsp143I GATC 1 cut(s) 703
BspACI CCGC 2 cut(s) 216, 552
BspANI GGCC 2 cut(s) 466, 678
BspCNI CTCAG 3 cut(s) 223, 409, 463
BspFNI CGCG 1 cut(s) 218
BspHI TCATGA 1 cut(s) 174
BspLI GGNNCC 3 cut(s) 123, 539, 705
BspMI ACCTGC 1 cut(s) 525
BspPI GGATC 2 cut(s) 698, 711
BspT104I TTCGAA 1 cut(s) 648
BspT107I GGYRCC 1 cut(s) 537
BspTNI GGTCTC 1 cut(s) 315
BsrDI GCAATG 1 cut(s) 744
BsrFI RCCGGY 1 cut(s) 118
BsrI ACTGG 3 cut(s) 458, 707, 762
BssAI RCCGGY 1 cut(s) 118
BssMI GATC 1 cut(s) 703
Bst2UI CCWGG 2 cut(s) 366, 542
Bst4CI ACNGT 3 cut(s) 25, 183, 439
Bst6I CTCTTC 1 cut(s) 693
BstBI TTCGAA 1 cut(s) 648
BstC8I GCNNGC 1 cut(s) 120
BstDEI CTNAG 4 cut(s) 11, 231, 417, 471
BstF5I GGATG 1 cut(s) 286
BstFNI CGCG 1 cut(s) 218
BstHHI GCGC 1 cut(s) 373
BstKTI GATC 1 cut(s) 706
BstMAI GTCTC 3 cut(s) 227, 315, 715
BstMBI GATC 1 cut(s) 703
BstMWI GCNNNNNNNGC 1 cut(s) 215
BstNI CCWGG 2 cut(s) 366, 542
BstSCI CCNGG 2 cut(s) 364, 540
BstUI CGCG 1 cut(s) 218
BstV1I GCAGC 4 cut(s) 221, 334, 425, 440
BstX2I RGATCY 1 cut(s) 703
BstXI CCANNNNNNTGG 1 cut(s) 408
BstYI RGATCY 1 cut(s) 703
BsuRI GGCC 2 cut(s) 466, 678
BtsCI GGATG 1 cut(s) 286
BtsIMutI CAGTG 4 cut(s) 179, 444, 451, 769
BveI ACCTGC 1 cut(s) 525
Cac8I GCNNGC 1 cut(s) 120
CciI TCATGA 1 cut(s) 174
CfoI GCGC 1 cut(s) 373
Cfr10I RCCGGY 1 cut(s) 118
Cfr13I GGNCC 1 cut(s) 464
Csp6I GTAC 3 cut(s) 86, 145, 538
CviAII CATG 3 cut(s) 175, 634, 723
CviQI GTAC 3 cut(s) 86, 145, 538
DdeI CTNAG 4 cut(s) 11, 231, 417, 471
DpnI GATC 1 cut(s) 705
DpnII GATC 1 cut(s) 703
EaeI YGGCCR 1 cut(s) 676
Eam1104I CTCTTC 1 cut(s) 693
EarI CTCTTC 1 cut(s) 693
Eco31I GGTCTC 1 cut(s) 315
EcoRI GAATTC 1 cut(s) 614
EcoRII CCWGG 2 cut(s) 364, 540
Esp3I CGTCTC 1 cut(s) 227
FaeI CATG 3 cut(s) 178, 637, 726
FatI CATG 3 cut(s) 174, 633, 722
FauI CCCGC 2 cut(s) 223, 545
Fnu4HI GCNGC 4 cut(s) 210, 323, 414, 429
FokI GGATG 1 cut(s) 293
Fsp4HI GCNGC 4 cut(s) 210, 323, 414, 429
GlaI GCGC 1 cut(s) 372
GluI GCNGC 4 cut(s) 210, 323, 414, 429
GsaI CCCAGC 1 cut(s) 759
GsuI CTGGAG 3 cut(s) 348, 563, 745
HaeIII GGCC 2 cut(s) 466, 678
HapII CCGG 1 cut(s) 119
HhaI GCGC 1 cut(s) 373
Hin1II CATG 3 cut(s) 178, 637, 726
Hin6I GCGC 1 cut(s) 371
HinP1I GCGC 1 cut(s) 371
HindIII AAGCTT 1 cut(s) 13
HinfI GANTC 3 cut(s) 171, 316, 637
HpaII CCGG 1 cut(s) 119
HphI GGTGA 2 cut(s) 511, 598
Hpy166II GTNNAC 1 cut(s) 62
Hpy188I TCNGA 4 cut(s) 192, 315, 472, 620
Hpy188III TCNNGA 4 cut(s) 111, 163, 175, 338
Hpy8I GTNNAC 1 cut(s) 62
HpyAV CCTTC 4 cut(s) 232, 238, 322, 477
HpyCH4III ACNGT 3 cut(s) 25, 183, 439
HpyCH4V TGCA 7 cut(s) 101, 209, 301, 428, 534, 582, 749
HpyF10VI GCNNNNNNNGC 1 cut(s) 215
HpyF3I CTNAG 4 cut(s) 11, 231, 417, 471
Hsp92II CATG 3 cut(s) 178, 637, 726
HspAI GCGC 1 cut(s) 371
KpnI GGTACC 1 cut(s) 541
KroI GCCGGC 1 cut(s) 118
KroNI GCCGGC 1 cut(s) 120
Kzo9I GATC 1 cut(s) 703
LmnI GCTCC 4 cut(s) 86, 127, 217, 764
Lsp1109I GCAGC 4 cut(s) 221, 334, 425, 440
LweI GCATC 1 cut(s) 288
MalI GATC 1 cut(s) 705
MboI GATC 1 cut(s) 703
MboII GAAGA 4 cut(s) 439, 595, 662, 710
MflI RGATCY 1 cut(s) 703
MlsI TGGCCA 1 cut(s) 678
MluCI AATT 4 cut(s) 29, 614, 643, 784
MluNI TGGCCA 1 cut(s) 678
MlyI GAGTC 1 cut(s) 325
MmeI TCCRAC 1 cut(s) 541
MnlI CCTC 5 cut(s) 159, 300, 372, 466, 694
Mox20I TGGCCA 1 cut(s) 678
MroNI GCCGGC 1 cut(s) 118
MscI TGGCCA 1 cut(s) 678
MseI TTAA 2 cut(s) 384, 629
Msp20I TGGCCA 1 cut(s) 678
MspA1I CMGCKG 2 cut(s) 416, 552
MspI CCGG 1 cut(s) 119
MspR9I CCNGG 2 cut(s) 366, 542
MvaI CCWGG 2 cut(s) 366, 542
MvnI CGCG 1 cut(s) 218
MwoI GCNNNNNNNGC 1 cut(s) 215
NaeI GCCGGC 1 cut(s) 120
NdeII GATC 1 cut(s) 703
NgoMIV GCCGGC 1 cut(s) 118
NlaIII CATG 3 cut(s) 178, 637, 726
NlaIV GGNNCC 3 cut(s) 123, 539, 705
NspV TTCGAA 1 cut(s) 648
PagI TCATGA 1 cut(s) 174
PdiI GCCGGC 1 cut(s) 120
PfeI GAWTC 2 cut(s) 171, 637
Pfl23II CGTACG 1 cut(s) 85
PflMI CCANNNNNTGG 1 cut(s) 457
PkrI GCNGC 4 cut(s) 211, 324, 415, 430
PleI GAGTC 1 cut(s) 324
PpsI GAGTC 1 cut(s) 324
Psp6I CCWGG 2 cut(s) 364, 540
PspFI CCCAGC 1 cut(s) 755
PspGI CCWGG 2 cut(s) 364, 540
PspLI CGTACG 1 cut(s) 85
PspN4I GGNNCC 3 cut(s) 123, 539, 705
PspPI GGNCC 1 cut(s) 464
PsuI RGATCY 1 cut(s) 703
PvuII CAGCTG 1 cut(s) 416
RsaI GTAC 3 cut(s) 87, 146, 539
RsaNI GTAC 3 cut(s) 86, 145, 538
SaqAI TTAA 2 cut(s) 384, 629
SatI GCNGC 4 cut(s) 210, 323, 414, 429
Sau3AI GATC 1 cut(s) 703
Sau96I GGNCC 1 cut(s) 464
SchI GAGTC 1 cut(s) 325
ScrFI CCNGG 2 cut(s) 366, 542
SfaNI GCATC 1 cut(s) 288
SfuI TTCGAA 1 cut(s) 648
Sse9I AATT 4 cut(s) 29, 614, 643, 784
SsiI CCGC 2 cut(s) 216, 552
StyD4I CCNGG 2 cut(s) 364, 540
TaaI ACNGT 3 cut(s) 25, 183, 439
TaqI TCGA 3 cut(s) 162, 337, 648
TasI AATT 4 cut(s) 29, 614, 643, 784
TatI WGTACW 1 cut(s) 144
TfiI GAWTC 2 cut(s) 171, 637
Tru1I TTAA 2 cut(s) 384, 629
Tru9I TTAA 2 cut(s) 384, 629
TscAI CASTG 4 cut(s) 186, 444, 458, 769
TseI GCWGC 4 cut(s) 209, 322, 413, 428
TspDTI ATGAA 4 cut(s) 163, 215, 509, 627
TspGWI ACGGA 1 cut(s) 73
TspRI CASTG 4 cut(s) 186, 444, 458, 769
Van91I CCANNNNNTGG 1 cut(s) 457
XapI RAATTY 2 cut(s) 614, 643
XcmI CCANNNNNNNNNTGG 2 cut(s) 405, 458
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.