pycom14g11240

Beta-carotene isomerase D27

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0016208
Physical Location & Seq
Forward (+)
14208005 .. 14210057
2053 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom14g11240.1

Sequence Viewer

Length: 651 bp
ATGTGTGGCCATAATTTTTTGTCACGAAAACAGAGGAAAACTCAACCAGAAATCTATTCAGAACCATCGGGAGAGCCGGCTCCAATGGGGCAAAAAACCAAGTACAACGATGGGTTTTTCGAGAGGGGATTCATGACTCTGTTTGCTCGGAAAATGATGAAGTTTGCAGCTCCCGCGAAACCGAGAACTGAGACGAAGGAGAAGGCGTGGTGGGAATATGACTACGAGAGCTTTGTGGATGTGTCCAAGAGAGTGATGCAGGGGAGGTCTCGGAGTCAGCAGCAGAAGGTGGTTCGAGAAGTGCTTTTGTCTATGCTGCCCCCAGGTGCGCCAGAGCAGTTTAAGAAACTATTTCCACCAACAAAGCGGGCAGCTGAGTTCAATGCTGCGTTGACAGTGCCTTTCTTCCACTGGTTGGTTGGGCCTTCTGAGGTTGTAGAAGTTGAGATAGATGGGGTGAAGCAAAGAAGTGGAGTTCATATAAAGAAATGCAGGTACCTGGAGAACAGCGGGTGTGTTGGAATGTGTGTGAATATGTGCAAGTTTCCTACACAAGATTTCTTCACCAATGAATTCGGACTTCCCTTAACCATGATTCCAAGTTCCATTGCAAATCCCAGCTCCAGTGTTTGTCCAAAACTACAAAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4033

Protein Analysis

217

Amino Acids

24.62

Weight (kDa)

9.39

Isoelectric Point (pI)

52.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
D27-like_C PF13225 138 - 201 2.4e-27 Beta-carotene isomerase D27-like, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016208)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G64680 AT1G64680
fragaria_vesca FvH4_5g10820
malus_domestica MD14G1137300.v1.1
prunus_persica Prupe.5G131200_v2.0.a1
pyrus_communis pycom14g11240
rosa_chinensis RchiOBHm_Chr7g0187341
rosa_laevigata RLG00000004793
rosa_roxburghii Rroxscaffold_3G00267590
rosa_rugosa Rorug06G0480700
rosa_samantha Rh7AG085200 Rh7BG077400 Rh7CG086200 Rh7DG087300
rosa_wichuraiana Rw7G007490

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 483
Acc65I GGTACC 1 cut(s) 495
AccB1I GGYRCC 1 cut(s) 495
AccB7I CCANNNNNTGG 1 cut(s) 415
AccII CGCG 1 cut(s) 176
AciI CCGC 3 cut(s) 174, 367, 510
AcoI YGGCCR 1 cut(s) 7
AcsI RAATTY 1 cut(s) 572
AfaI GTAC 2 cut(s) 104, 497
AfiI CCNNNNNNNGG 1 cut(s) 415
AgsI TTSAA 1 cut(s) 382
AjnI CCWGG 2 cut(s) 322, 498
AluBI AGCT 4 cut(s) 170, 231, 374, 621
AluI AGCT 4 cut(s) 170, 231, 374, 621
Alw26I GTCTC 2 cut(s) 185, 273
AoxI GGCC 2 cut(s) 7, 422
ApeKI GCWGC 5 cut(s) 167, 280, 316, 371, 386
ApoI RAATTY 1 cut(s) 572
Asp718I GGTACC 1 cut(s) 495
AspLEI GCGC 1 cut(s) 331
AspS9I GGNCC 1 cut(s) 422
AsuHPI GGTGA 2 cut(s) 469, 556
BalI TGGCCA 1 cut(s) 9
BanI GGYRCC 1 cut(s) 495
BbvI GCAGC 5 cut(s) 179, 292, 303, 373, 383
BccI CCATC 3 cut(s) 73, 104, 446
BciT130I CCWGG 2 cut(s) 324, 500
BcoDI GTCTC 2 cut(s) 185, 273
BfuAI ACCTGC 1 cut(s) 483
BisI GCNGC 5 cut(s) 168, 281, 317, 372, 387
BlsI GCNGC 5 cut(s) 169, 282, 318, 373, 388
Bme1390I CCNGG 2 cut(s) 324, 500
BmgT120I GGNCC 1 cut(s) 422
BmiI GGNNCC 2 cut(s) 81, 497
BmrFI CCNGG 2 cut(s) 324, 500
BmsI GCATC 1 cut(s) 246
BplI GAGNNNNNCTC 2 cut(s) 25, 57
BpmI CTGGAG 2 cut(s) 521, 607
BsaI GGTCTC 1 cut(s) 273
BsaJI CCNNGG 1 cut(s) 322
BsaXI ACNNNNNCTCC 2 cut(s) 191, 221
Bsc4I CCNNNNNNNGG 1 cut(s) 415
Bse118I RCCGGY 1 cut(s) 76
Bse1I ACTGG 2 cut(s) 416, 624
Bse3DI GCAATG 1 cut(s) 606
BseBI CCWGG 2 cut(s) 324, 500
BseDI CCNNGG 1 cut(s) 322
BseGI GGATG 1 cut(s) 244
BseLI CCNNNNNNNGG 1 cut(s) 415
BseMI GCAATG 1 cut(s) 606
BseMII CTCAG 3 cut(s) 180, 366, 420
BseNI ACTGG 2 cut(s) 416, 624
BseXI GCAGC 5 cut(s) 179, 292, 303, 373, 383
BseYI CCCAGC 1 cut(s) 617
Bsh1236I CGCG 1 cut(s) 176
BshFI GGCC 2 cut(s) 9, 424
BshNI GGYRCC 1 cut(s) 495
BsiSI CCGG 1 cut(s) 77
BslI CCNNNNNNNGG 1 cut(s) 415
BsmAI GTCTC 2 cut(s) 185, 273
BsmBI CGTCTC 1 cut(s) 185
BsnI GGCC 2 cut(s) 9, 424
Bso31I GGTCTC 1 cut(s) 273
BspACI CCGC 3 cut(s) 174, 367, 510
BspANI GGCC 2 cut(s) 9, 424
BspCNI CTCAG 3 cut(s) 181, 367, 421
BspFNI CGCG 1 cut(s) 176
BspHI TCATGA 1 cut(s) 132
BspLI GGNNCC 2 cut(s) 81, 497
BspMI ACCTGC 1 cut(s) 483
BspT107I GGYRCC 1 cut(s) 495
BspTNI GGTCTC 1 cut(s) 273
BsrDI GCAATG 1 cut(s) 606
BsrFI RCCGGY 1 cut(s) 76
BsrI ACTGG 2 cut(s) 416, 624
BssAI RCCGGY 1 cut(s) 76
BssECI CCNNGG 1 cut(s) 322
Bst2UI CCWGG 2 cut(s) 324, 500
Bst4CI ACNGT 1 cut(s) 397
BstC8I GCNNGC 2 cut(s) 78, 369
BstDEI CTNAG 3 cut(s) 189, 375, 429
BstF5I GGATG 1 cut(s) 244
BstFNI CGCG 1 cut(s) 176
BstHHI GCGC 1 cut(s) 331
BstMAI GTCTC 2 cut(s) 185, 273
BstMWI GCNNNNNNNGC 1 cut(s) 173
BstNI CCWGG 2 cut(s) 324, 500
BstSCI CCNGG 2 cut(s) 322, 498
BstUI CGCG 1 cut(s) 176
BstV1I GCAGC 5 cut(s) 179, 292, 303, 373, 383
BsuRI GGCC 2 cut(s) 9, 424
BtsCI GGATG 1 cut(s) 244
BtsIMutI CAGTG 3 cut(s) 402, 409, 631
BveI ACCTGC 1 cut(s) 483
Cac8I GCNNGC 2 cut(s) 78, 369
CciI TCATGA 1 cut(s) 132
CfoI GCGC 1 cut(s) 331
Cfr10I RCCGGY 1 cut(s) 76
Cfr13I GGNCC 1 cut(s) 422
Csp6I GTAC 2 cut(s) 103, 496
CviAII CATG 2 cut(s) 133, 592
CviJI RGCY 8 cut(s) 9, 76, 80, 170, 231, 374, 424, 621
CviKI_1 RGCY 8 cut(s) 9, 76, 80, 170, 231, 374, 424, 621
CviQI GTAC 2 cut(s) 103, 496
DdeI CTNAG 3 cut(s) 189, 375, 429
EaeI YGGCCR 1 cut(s) 7
Eco31I GGTCTC 1 cut(s) 273
EcoRI GAATTC 1 cut(s) 572
EcoRII CCWGG 2 cut(s) 322, 498
Esp3I CGTCTC 1 cut(s) 185
FaeI CATG 2 cut(s) 136, 595
FaiI YATR 8 cut(s) 12, 134, 219, 314, 480, 482, 536, 593
FatI CATG 2 cut(s) 132, 591
FauI CCCGC 3 cut(s) 181, 360, 503
Fnu4HI GCNGC 5 cut(s) 168, 281, 317, 372, 387
FokI GGATG 1 cut(s) 251
Fsp4HI GCNGC 5 cut(s) 168, 281, 317, 372, 387
GlaI GCGC 1 cut(s) 330
GluI GCNGC 5 cut(s) 168, 281, 317, 372, 387
GsaI CCCAGC 1 cut(s) 621
GsuI CTGGAG 2 cut(s) 521, 607
HaeIII GGCC 2 cut(s) 9, 424
HapII CCGG 1 cut(s) 77
HhaI GCGC 1 cut(s) 331
Hin1II CATG 2 cut(s) 136, 595
Hin6I GCGC 1 cut(s) 329
HinP1I GCGC 1 cut(s) 329
HincII GTYRAC 1 cut(s) 393
HindII GTYRAC 1 cut(s) 393
HinfI GANTC 4 cut(s) 129, 136, 274, 595
HpaII CCGG 1 cut(s) 77
HphI GGTGA 2 cut(s) 469, 556
Hpy166II GTNNAC 1 cut(s) 393
Hpy188I TCNGA 5 cut(s) 61, 150, 273, 430, 578
Hpy188III TCNNGA 5 cut(s) 24, 69, 121, 133, 296
Hpy8I GTNNAC 1 cut(s) 393
HpyAV CCTTC 4 cut(s) 190, 196, 280, 435
HpyCH4III ACNGT 1 cut(s) 397
HpyCH4V TGCA 5 cut(s) 167, 259, 492, 540, 611
HpyF10VI GCNNNNNNNGC 1 cut(s) 173
HpyF3I CTNAG 3 cut(s) 189, 375, 429
Hsp92II CATG 2 cut(s) 136, 595
HspAI GCGC 1 cut(s) 329
KpnI GGTACC 1 cut(s) 499
KroI GCCGGC 1 cut(s) 76
KroNI GCCGGC 1 cut(s) 78
LmnI GCTCC 3 cut(s) 85, 175, 626
Lsp1109I GCAGC 5 cut(s) 179, 292, 303, 373, 383
LweI GCATC 1 cut(s) 246
MaeIII GTNAC 1 cut(s) 21
MboII GAAGA 2 cut(s) 397, 553
MlsI TGGCCA 1 cut(s) 9
MluCI AATT 3 cut(s) 13, 572, 646
MluNI TGGCCA 1 cut(s) 9
MlyI GAGTC 2 cut(s) 130, 283
MmeI TCCRAC 1 cut(s) 499
MnlI CCTC 4 cut(s) 27, 117, 258, 424
Mox20I TGGCCA 1 cut(s) 9
MroNI GCCGGC 1 cut(s) 76
MscI TGGCCA 1 cut(s) 9
MseI TTAA 2 cut(s) 342, 587
Msp20I TGGCCA 1 cut(s) 9
MspA1I CMGCKG 2 cut(s) 374, 510
MspI CCGG 1 cut(s) 77
MspR9I CCNGG 2 cut(s) 324, 500
MvaI CCWGG 2 cut(s) 324, 500
MvnI CGCG 1 cut(s) 176
MwoI GCNNNNNNNGC 1 cut(s) 173
NaeI GCCGGC 1 cut(s) 78
NgoMIV GCCGGC 1 cut(s) 76
NlaIII CATG 2 cut(s) 136, 595
NlaIV GGNNCC 2 cut(s) 81, 497
NmuCI GTSAC 1 cut(s) 21
PagI TCATGA 1 cut(s) 132
PdiI GCCGGC 1 cut(s) 78
PfeI GAWTC 2 cut(s) 129, 595
PflMI CCANNNNNTGG 1 cut(s) 415
PkrI GCNGC 5 cut(s) 169, 282, 318, 373, 388
PleI GAGTC 2 cut(s) 130, 282
PpsI GAGTC 2 cut(s) 130, 282
Psp6I CCWGG 2 cut(s) 322, 498
PspFI CCCAGC 1 cut(s) 617
PspGI CCWGG 2 cut(s) 322, 498
PspN4I GGNNCC 2 cut(s) 81, 497
PspPI GGNCC 1 cut(s) 422
PvuII CAGCTG 1 cut(s) 374
RsaI GTAC 2 cut(s) 104, 497
RsaNI GTAC 2 cut(s) 103, 496
SaqAI TTAA 2 cut(s) 342, 587
SatI GCNGC 5 cut(s) 168, 281, 317, 372, 387
Sau96I GGNCC 1 cut(s) 422
SchI GAGTC 2 cut(s) 130, 283
ScrFI CCNGG 2 cut(s) 324, 500
SfaNI GCATC 1 cut(s) 246
Sse9I AATT 3 cut(s) 13, 572, 646
SsiI CCGC 3 cut(s) 174, 367, 510
StyD4I CCNGG 2 cut(s) 322, 498
TaaI ACNGT 1 cut(s) 397
TaqI TCGA 2 cut(s) 120, 295
TasI AATT 3 cut(s) 13, 572, 646
TatI WGTACW 1 cut(s) 102
TfiI GAWTC 2 cut(s) 129, 595
Tru1I TTAA 2 cut(s) 342, 587
Tru9I TTAA 2 cut(s) 342, 587
TscAI CASTG 3 cut(s) 402, 416, 631
TseFI GTSAC 1 cut(s) 21
TseI GCWGC 5 cut(s) 167, 280, 316, 371, 386
Tsp45I GTSAC 1 cut(s) 21
TspDTI ATGAA 4 cut(s) 121, 173, 467, 585
TspRI CASTG 3 cut(s) 402, 416, 631
Van91I CCANNNNNTGG 1 cut(s) 415
XapI RAATTY 1 cut(s) 572
XcmI CCANNNNNNNNNTGG 1 cut(s) 416
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.