Prupe.5G131200_v2.0.a1

Beta-carotene isomerase D27

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp05
OG0016208
Physical Location & Seq
Forward (+)
12657086 .. 12660269
3184 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.5G131200.1

Sequence Viewer

Length: 789 bp
ATGGTGGTTCTAAGCCTTCAACCTGTCCAATTTTCTACCCCTAAACACATGTTATTATGTAGACCTGGCAGTACTGGTTATCCCATCCGATGTGGGATTGCAGAGCCATCAGGAGAGCCAGCTCCCATGGGACAAAAGACCAAGTACAATGATGGGTTTTTTGAGAAGGGATTTATGACACTGTTTGCTCGCAAAATGAAGAAGTTTGCAGCTCCAGTGAAGGCCAGGACTGAGACTAAGGAGAAGGCATGGTGGGAATATGACTATGAGAGCTTTGTGGATGTCTCTAAGAGAGTGATGCAGGGGAGGTCTAGAGTTCAGCAGCAGCAAGTAGTTCGAGAGGTGCTCTTGTCTATGCTGCCTCCAGGTGCGCCTGCTCAGTTTAGGAAATTGTTTCCACCAACAAAATGGGCAGCTGAATTCAATGCTGCATTAACAGTGCCTTTCTTCCACTGGTTGGTTGGGCCTTCTGAGGTTGTAGAAGTTGAGATAAATGGGGTGAAGCAAAGAAGTGGAGTCCATATAAAGAAATGCAGGTACCTGGAGAACAGTGGGTGTGTTGGAATGTGTGTGAATATGTGCAAGTTTCCTACTCAAGATTTCTTCACCAATGAATTTGGGCTTCCCTTAACCATGATTCCAAATTTTGAAGATATGAGTTGTGAAATGGTGTATGGCCAAGTTCCACCACCATTTGAAGAGGATCCAGTGTCCAAACAACCATGTTTTACTGATATATGTTCCATTGCAACTCCCAGCTCCAGTGTCTGTCCAAAATTACAAGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4033

Protein Analysis

263

Amino Acids

29.5

Weight (kDa)

8.63

Isoelectric Point (pI)

47.36

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016208)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G64680 AT1G64680
fragaria_vesca FvH4_5g10820
malus_domestica MD14G1137300.v1.1
prunus_persica Prupe.5G131200_v2.0.a1
pyrus_communis pycom14g11240
rosa_chinensis RchiOBHm_Chr7g0187341
rosa_laevigata RLG00000004793
rosa_roxburghii Rroxscaffold_3G00267590
rosa_rugosa Rorug06G0480700
rosa_samantha Rh7AG085200 Rh7BG077400 Rh7CG086200 Rh7DG087300
rosa_wichuraiana Rw7G007490

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 525
Acc65I GGTACC 1 cut(s) 537
AccB1I GGYRCC 1 cut(s) 537
AccB7I CCANNNNNTGG 1 cut(s) 457
AccI GTMKAC 1 cut(s) 61
AclWI GGATC 2 cut(s) 698, 711
AcoI YGGCCR 1 cut(s) 676
AcsI RAATTY 3 cut(s) 419, 614, 643
AfaI GTAC 3 cut(s) 73, 146, 539
AfiI CCNNNNNNNGG 1 cut(s) 457
AflIII ACRYGT 1 cut(s) 48
AgsI TTSAA 4 cut(s) 20, 424, 650, 698
AjnI CCWGG 4 cut(s) 64, 224, 364, 540
AluBI AGCT 6 cut(s) 122, 212, 273, 416, 759, 785
AluI AGCT 6 cut(s) 122, 212, 273, 416, 759, 785
Alw21I GWGCWC 1 cut(s) 348
Alw26I GTCTC 2 cut(s) 227, 289
AlwI GGATC 2 cut(s) 698, 711
AlwNI CAGNNNCTG 1 cut(s) 768
AoxI GGCC 3 cut(s) 222, 464, 676
ApeKI GCWGC 6 cut(s) 209, 322, 325, 358, 413, 428
ApoI RAATTY 3 cut(s) 419, 614, 643
Asp718I GGTACC 1 cut(s) 537
AspLEI GCGC 1 cut(s) 373
AspS9I GGNCC 1 cut(s) 464
AsuHPI GGTGA 2 cut(s) 511, 598
BalI TGGCCA 1 cut(s) 678
BamHI GGATCC 1 cut(s) 703
BanI GGYRCC 1 cut(s) 537
Bbv12I GWGCWC 1 cut(s) 348
BbvI GCAGC 6 cut(s) 221, 334, 337, 345, 415, 425
BccI CCATC 3 cut(s) 92, 115, 146
BcgI CGANNNNNNTGC 2 cut(s) 317, 351
BciT130I CCWGG 4 cut(s) 66, 226, 366, 542
BcoDI GTCTC 2 cut(s) 227, 289
BfaI CTAG 1 cut(s) 312
BfuAI ACCTGC 1 cut(s) 525
BisI GCNGC 6 cut(s) 210, 323, 326, 359, 414, 429
BlsI GCNGC 6 cut(s) 211, 324, 327, 360, 415, 430
BmcAI AGTACT 1 cut(s) 73
Bme1390I CCNGG 4 cut(s) 66, 226, 366, 542
BmgT120I GGNCC 1 cut(s) 464
BmiI GGNNCC 2 cut(s) 539, 705
BmrFI CCNGG 4 cut(s) 66, 226, 366, 542
BmsI GCATC 1 cut(s) 288
BplI GAGNNNNNCTC 2 cut(s) 330, 362
BpmI CTGGAG 4 cut(s) 198, 348, 563, 745
BpuEI CTTGAG 1 cut(s) 579
BsaJI CCNNGG 1 cut(s) 126
Bsc4I CCNNNNNNNGG 1 cut(s) 457
Bse1I ACTGG 5 cut(s) 79, 215, 458, 707, 762
Bse3DI GCAATG 1 cut(s) 744
BseBI CCWGG 4 cut(s) 66, 226, 366, 542
BseDI CCNNGG 1 cut(s) 126
BseGI GGATG 2 cut(s) 84, 286
BseLI CCNNNNNNNGG 1 cut(s) 457
BseMI GCAATG 1 cut(s) 744
BseMII CTCAG 3 cut(s) 222, 392, 462
BseNI ACTGG 5 cut(s) 79, 215, 458, 707, 762
BseXI GCAGC 6 cut(s) 221, 334, 337, 345, 415, 425
BseYI CCCAGC 1 cut(s) 755
BshFI GGCC 3 cut(s) 224, 466, 678
BshNI GGYRCC 1 cut(s) 537
BsiHKAI GWGCWC 1 cut(s) 348
BslFI GGGAC 1 cut(s) 144
BslI CCNNNNNNNGG 1 cut(s) 457
BsmAI GTCTC 2 cut(s) 227, 289
BsmFI GGGAC 1 cut(s) 144
BsnI GGCC 3 cut(s) 224, 466, 678
Bsp1286I GDGCHC 1 cut(s) 348
Bsp143I GATC 1 cut(s) 703
Bsp19I CCATGG 1 cut(s) 126
BspANI GGCC 3 cut(s) 224, 466, 678
BspCNI CTCAG 3 cut(s) 223, 391, 463
BspLI GGNNCC 2 cut(s) 539, 705
BspMI ACCTGC 1 cut(s) 525
BspPI GGATC 2 cut(s) 698, 711
BspT107I GGYRCC 1 cut(s) 537
BsrDI GCAATG 1 cut(s) 744
BsrI ACTGG 5 cut(s) 79, 215, 458, 707, 762
BssECI CCNNGG 1 cut(s) 126
BssMI GATC 1 cut(s) 703
BssT1I CCWWGG 1 cut(s) 126
Bst2UI CCWGG 4 cut(s) 66, 226, 366, 542
Bst4CI ACNGT 3 cut(s) 183, 439, 551
Bst6I CTCTTC 1 cut(s) 693
BstC8I GCNNGC 3 cut(s) 120, 190, 375
BstDEI CTNAG 6 cut(s) 11, 231, 237, 288, 378, 471
BstDSI CCRYGG 1 cut(s) 126
BstF5I GGATG 2 cut(s) 84, 286
BstHHI GCGC 1 cut(s) 373
BstKTI GATC 1 cut(s) 706
BstMAI GTCTC 2 cut(s) 227, 289
BstMBI GATC 1 cut(s) 703
BstNI CCWGG 4 cut(s) 66, 226, 366, 542
BstNSI RCATGY 1 cut(s) 52
BstSCI CCNGG 4 cut(s) 64, 224, 364, 540
BstV1I GCAGC 6 cut(s) 221, 334, 337, 345, 415, 425
BstX2I RGATCY 1 cut(s) 703
BstXI CCANNNNNNTGG 1 cut(s) 408
BstYI RGATCY 1 cut(s) 703
BsuRI GGCC 3 cut(s) 224, 466, 678
BtgI CCRYGG 1 cut(s) 126
BtsCI GGATG 2 cut(s) 84, 286
BtsIMutI CAGTG 7 cut(s) 179, 222, 444, 451, 556, 714, 769
BveI ACCTGC 1 cut(s) 525
Cac8I GCNNGC 3 cut(s) 120, 190, 375
CaiI CAGNNNCTG 1 cut(s) 768
CfoI GCGC 1 cut(s) 373
Cfr13I GGNCC 1 cut(s) 464
Csp6I GTAC 3 cut(s) 72, 145, 538
CviAII CATG 5 cut(s) 49, 127, 249, 634, 723
CviQI GTAC 3 cut(s) 72, 145, 538
DdeI CTNAG 6 cut(s) 11, 231, 237, 288, 378, 471
DpnI GATC 1 cut(s) 705
DpnII GATC 1 cut(s) 703
EaeI YGGCCR 1 cut(s) 676
Eam1104I CTCTTC 1 cut(s) 693
EarI CTCTTC 1 cut(s) 693
Eco130I CCWWGG 1 cut(s) 126
EcoRI GAATTC 1 cut(s) 419
EcoRII CCWGG 4 cut(s) 64, 224, 364, 540
EcoT14I CCWWGG 1 cut(s) 126
ErhI CCWWGG 1 cut(s) 126
FaeI CATG 5 cut(s) 52, 130, 252, 637, 726
FaqI GGGAC 1 cut(s) 144
FatI CATG 5 cut(s) 48, 126, 248, 633, 722
FblI GTMKAC 1 cut(s) 61
Fnu4HI GCNGC 6 cut(s) 210, 323, 326, 359, 414, 429
FokI GGATG 2 cut(s) 71, 293
Fsp4HI GCNGC 6 cut(s) 210, 323, 326, 359, 414, 429
FspBI CTAG 1 cut(s) 312
GlaI GCGC 1 cut(s) 372
GluI GCNGC 6 cut(s) 210, 323, 326, 359, 414, 429
GsaI CCCAGC 1 cut(s) 759
GsuI CTGGAG 4 cut(s) 198, 348, 563, 745
HaeIII GGCC 3 cut(s) 224, 466, 678
HhaI GCGC 1 cut(s) 373
Hin1II CATG 5 cut(s) 52, 130, 252, 637, 726
Hin6I GCGC 1 cut(s) 371
HinP1I GCGC 1 cut(s) 371
HindIII AAGCTT 1 cut(s) 783
HinfI GANTC 2 cut(s) 516, 637
HphI GGTGA 2 cut(s) 511, 598
Hpy166II GTNNAC 1 cut(s) 62
Hpy188I TCNGA 2 cut(s) 89, 472
Hpy188III TCNNGA 4 cut(s) 111, 312, 338, 596
Hpy8I GTNNAC 1 cut(s) 62
HpyAV CCTTC 5 cut(s) 26, 160, 214, 238, 477
HpyCH4III ACNGT 3 cut(s) 183, 439, 551
HpyCH4V TGCA 7 cut(s) 101, 209, 301, 431, 534, 582, 749
HpyF3I CTNAG 6 cut(s) 11, 231, 237, 288, 378, 471
Hsp92II CATG 5 cut(s) 52, 130, 252, 637, 726
HspAI GCGC 1 cut(s) 371
KpnI GGTACC 1 cut(s) 541
Kzo9I GATC 1 cut(s) 703
LmnI GCTCC 3 cut(s) 127, 217, 764
Lsp1109I GCAGC 6 cut(s) 221, 334, 337, 345, 415, 425
LweI GCATC 1 cut(s) 288
MaeI CTAG 1 cut(s) 312
MalI GATC 1 cut(s) 705
MboI GATC 1 cut(s) 703
MboII GAAGA 5 cut(s) 211, 439, 595, 662, 710
MflI RGATCY 1 cut(s) 703
MhlI GDGCHC 1 cut(s) 348
MlsI TGGCCA 1 cut(s) 678
MluCI AATT 6 cut(s) 29, 389, 419, 614, 643, 776
MluNI TGGCCA 1 cut(s) 678
MlyI GAGTC 1 cut(s) 525
MmeI TCCRAC 1 cut(s) 541
MnlI CCTC 5 cut(s) 300, 334, 372, 466, 694
Mox20I TGGCCA 1 cut(s) 678
MscI TGGCCA 1 cut(s) 678
MseI TTAA 2 cut(s) 434, 629
Msp20I TGGCCA 1 cut(s) 678
MspA1I CMGCKG 1 cut(s) 416
MspR9I CCNGG 4 cut(s) 66, 226, 366, 542
MvaI CCWGG 4 cut(s) 66, 226, 366, 542
NcoI CCATGG 1 cut(s) 126
NdeII GATC 1 cut(s) 703
NlaIII CATG 5 cut(s) 52, 130, 252, 637, 726
NlaIV GGNNCC 2 cut(s) 539, 705
NspI RCATGY 1 cut(s) 52
PciI ACATGT 1 cut(s) 48
PfeI GAWTC 1 cut(s) 637
PflMI CCANNNNNTGG 1 cut(s) 457
PkrI GCNGC 6 cut(s) 211, 324, 327, 360, 415, 430
PleI GAGTC 1 cut(s) 524
PpsI GAGTC 1 cut(s) 524
PscI ACATGT 1 cut(s) 48
Psp6I CCWGG 4 cut(s) 64, 224, 364, 540
PspFI CCCAGC 1 cut(s) 755
PspGI CCWGG 4 cut(s) 64, 224, 364, 540
PspN4I GGNNCC 2 cut(s) 539, 705
PspPI GGNCC 1 cut(s) 464
PstNI CAGNNNCTG 1 cut(s) 768
PsuI RGATCY 1 cut(s) 703
PvuII CAGCTG 1 cut(s) 416
RsaI GTAC 3 cut(s) 73, 146, 539
RsaNI GTAC 3 cut(s) 72, 145, 538
SaqAI TTAA 2 cut(s) 434, 629
SatI GCNGC 6 cut(s) 210, 323, 326, 359, 414, 429
Sau3AI GATC 1 cut(s) 703
Sau96I GGNCC 1 cut(s) 464
ScaI AGTACT 1 cut(s) 73
SchI GAGTC 1 cut(s) 525
ScrFI CCNGG 4 cut(s) 66, 226, 366, 542
SduI GDGCHC 1 cut(s) 348
SfaNI GCATC 1 cut(s) 288
SmlI CTYRAG 1 cut(s) 594
SmoI CTYRAG 1 cut(s) 594
Sse9I AATT 6 cut(s) 29, 389, 419, 614, 643, 776
SspMI CTAG 1 cut(s) 312
StyD4I CCNGG 4 cut(s) 64, 224, 364, 540
StyI CCWWGG 1 cut(s) 126
TaaI ACNGT 3 cut(s) 183, 439, 551
TaqI TCGA 1 cut(s) 337
TasI AATT 6 cut(s) 29, 389, 419, 614, 643, 776
TatI WGTACW 2 cut(s) 71, 144
TfiI GAWTC 1 cut(s) 637
Tru1I TTAA 2 cut(s) 434, 629
Tru9I TTAA 2 cut(s) 434, 629
TscAI CASTG 7 cut(s) 186, 222, 444, 458, 556, 714, 769
TseI GCWGC 6 cut(s) 209, 322, 325, 358, 413, 428
TspDTI ATGAA 2 cut(s) 212, 627
TspRI CASTG 7 cut(s) 186, 222, 444, 458, 556, 714, 769
Van91I CCANNNNNTGG 1 cut(s) 457
XapI RAATTY 3 cut(s) 419, 614, 643
XbaI TCTAGA 1 cut(s) 311
XceI RCATGY 1 cut(s) 52
XcmI CCANNNNNNNNNTGG 2 cut(s) 405, 458
XmiI GTMKAC 1 cut(s) 61
XspI CTAG 1 cut(s) 312
ZrmI AGTACT 1 cut(s) 73
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.