RLG00000004793

Beta-carotene isomerase D27

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0016208
Physical Location & Seq
Reverse (-)
62312229 .. 62313794
1566 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000004793

Sequence Viewer

Length: 615 bp
ATGACACTCTTCGCTCGCAAAATGAAGAGGTTTGCAGCTCCAGTGAAATCCAAGACTGAGATTGAGGAAAAGAAATGGTGGGAATATGACTATGAGAGTTTTGTGGACGTCTCCAAGAGAGTTATGCAGGGAAGGTCTCGCATTCAGCAGCAACAAGTGGTTAGGGAGGTCTTGTTATCTATGCTACCTCCAGGTGCGCCTGCTCAGTTTAGGAAATTATTTCCACCAACAAAGTGGGCTGCAGAGTTCAATGCTGCATTAACTGTGCCTTTCTTCCACTGGTTAGTTGGGCCTTCTGAGGTTATAGAAGTTGAGGTAGAAGGAGCGAAGCAAAGAAGTGGAGTTCGTATAAAGAAATGCAGGTACCTGGAGAACAGCGGCTGCGTTGGAATGTGTGTGAATATGTGCAAGTTTCCTACTCAAGATTTCTTCACCAACGAATTTGGGCTTCCATTAACCATGATTCCAAATTTTGAAGATATGAGTTGTGATATGGTGTACGGCCAAGTTCCACCACCGTTTGAAGAGGATCCAGTGTCCAAACAACCCTGTTTTACTGATATATGCTCCATTGCGAATCCCTGCGCCACTGTCTGTCCAAAGTTACAAGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4033

Protein Analysis

205

Amino Acids

23.29

Weight (kDa)

8.04

Isoelectric Point (pI)

54.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
D27-like_C PF13225 94 - 176 3.6e-38 Beta-carotene isomerase D27-like, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016208)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G64680 AT1G64680
fragaria_vesca FvH4_5g10820
malus_domestica MD14G1137300.v1.1
prunus_persica Prupe.5G131200_v2.0.a1
pyrus_communis pycom14g11240
rosa_chinensis RchiOBHm_Chr7g0187341
rosa_laevigata RLG00000004793
rosa_roxburghii Rroxscaffold_3G00267590
rosa_rugosa Rorug06G0480700
rosa_samantha Rh7AG085200 Rh7BG077400 Rh7CG086200 Rh7DG087300
rosa_wichuraiana Rw7G007490

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 111
Acc36I ACCTGC 1 cut(s) 351
Acc65I GGTACC 1 cut(s) 363
AccB1I GGYRCC 1 cut(s) 363
AciI CCGC 1 cut(s) 378
AclWI GGATC 2 cut(s) 524, 537
AcoI YGGCCR 1 cut(s) 502
AcsI RAATTY 2 cut(s) 440, 469
AcyI GRCGYC 1 cut(s) 108
AfaI GTAC 2 cut(s) 365, 500
AgsI TTSAA 3 cut(s) 250, 476, 524
AjnI CCWGG 2 cut(s) 190, 366
AluBI AGCT 2 cut(s) 38, 611
AluI AGCT 2 cut(s) 38, 611
Alw26I GTCTC 2 cut(s) 115, 141
AlwI GGATC 2 cut(s) 524, 537
AlwNI CAGNNNCTG 1 cut(s) 381
AoxI GGCC 2 cut(s) 290, 502
ApeKI GCWGC 5 cut(s) 35, 148, 239, 254, 381
ApoI RAATTY 2 cut(s) 440, 469
Asp718I GGTACC 1 cut(s) 363
AspLEI GCGC 2 cut(s) 199, 587
AspS9I GGNCC 1 cut(s) 290
AsuHPI GGTGA 1 cut(s) 424
BamHI GGATCC 1 cut(s) 529
BanI GGYRCC 1 cut(s) 363
BbvI GCAGC 5 cut(s) 47, 160, 226, 241, 368
BceAI ACGGC 1 cut(s) 517
BciT130I CCWGG 2 cut(s) 192, 368
BcoDI GTCTC 2 cut(s) 115, 141
BfmI CTRYAG 1 cut(s) 240
BfuAI ACCTGC 1 cut(s) 351
BisI GCNGC 6 cut(s) 36, 149, 240, 255, 379, 382
BlsI GCNGC 6 cut(s) 37, 150, 241, 256, 380, 383
Bme1390I CCNGG 2 cut(s) 192, 368
BmgT120I GGNCC 1 cut(s) 290
BmiI GGNNCC 2 cut(s) 365, 531
BmrFI CCNGG 2 cut(s) 192, 368
BpmI CTGGAG 3 cut(s) 24, 174, 389
BpuEI CTTGAG 1 cut(s) 405
BsaHI GRCGYC 1 cut(s) 108
BsaI GGTCTC 1 cut(s) 141
BsaXI ACNNNNNCTCC 2 cut(s) 158, 188
Bse1I ACTGG 3 cut(s) 41, 284, 533
Bse3DI GCAATG 1 cut(s) 570
BseBI CCWGG 2 cut(s) 192, 368
BseMI GCAATG 1 cut(s) 570
BseMII CTCAG 3 cut(s) 48, 218, 288
BseNI ACTGG 3 cut(s) 41, 284, 533
BseXI GCAGC 5 cut(s) 47, 160, 226, 241, 368
BshFI GGCC 2 cut(s) 292, 504
BshNI GGYRCC 1 cut(s) 363
BsmAI GTCTC 2 cut(s) 115, 141
BsmBI CGTCTC 1 cut(s) 115
BsmI GAATGC 1 cut(s) 141
BsnI GGCC 2 cut(s) 292, 504
Bso31I GGTCTC 1 cut(s) 141
Bsp143I GATC 1 cut(s) 529
BspACI CCGC 1 cut(s) 378
BspANI GGCC 2 cut(s) 292, 504
BspCNI CTCAG 3 cut(s) 49, 217, 289
BspLI GGNNCC 2 cut(s) 365, 531
BspMAI CTGCAG 1 cut(s) 244
BspMI ACCTGC 1 cut(s) 351
BspPI GGATC 2 cut(s) 524, 537
BspT107I GGYRCC 1 cut(s) 363
BspTNI GGTCTC 1 cut(s) 141
BsrDI GCAATG 1 cut(s) 570
BsrI ACTGG 3 cut(s) 41, 284, 533
BssMI GATC 1 cut(s) 529
BssNI GRCGYC 1 cut(s) 108
Bst2UI CCWGG 2 cut(s) 192, 368
Bst4CI ACNGT 3 cut(s) 265, 519, 592
Bst6I CTCTTC 3 cut(s) 14, 20, 519
BstACI GRCGYC 1 cut(s) 108
BstC8I GCNNGC 2 cut(s) 16, 201
BstDEI CTNAG 3 cut(s) 57, 204, 297
BstHHI GCGC 2 cut(s) 199, 587
BstKTI GATC 1 cut(s) 532
BstMAI GTCTC 2 cut(s) 115, 141
BstMBI GATC 1 cut(s) 529
BstNI CCWGG 2 cut(s) 192, 368
BstSCI CCNGG 2 cut(s) 190, 366
BstSFI CTRYAG 1 cut(s) 240
BstV1I GCAGC 5 cut(s) 47, 160, 226, 241, 368
BstX2I RGATCY 1 cut(s) 529
BstXI CCANNNNNNTGG 1 cut(s) 234
BstYI RGATCY 1 cut(s) 529
BsuRI GGCC 2 cut(s) 292, 504
BtsIMutI CAGTG 4 cut(s) 48, 277, 540, 588
BveI ACCTGC 1 cut(s) 351
Cac8I GCNNGC 2 cut(s) 16, 201
CaiI CAGNNNCTG 1 cut(s) 381
CfoI GCGC 2 cut(s) 199, 587
Cfr13I GGNCC 1 cut(s) 290
Csp6I GTAC 2 cut(s) 364, 499
CviAII CATG 1 cut(s) 460
CviJI RGCY 7 cut(s) 38, 239, 292, 381, 448, 504, 611
CviKI_1 RGCY 7 cut(s) 38, 239, 292, 381, 448, 504, 611
CviQI GTAC 2 cut(s) 364, 499
DdeI CTNAG 3 cut(s) 57, 204, 297
DpnI GATC 1 cut(s) 531
DpnII GATC 1 cut(s) 529
EaeI YGGCCR 1 cut(s) 502
Eam1104I CTCTTC 3 cut(s) 14, 20, 519
EarI CTCTTC 3 cut(s) 14, 20, 519
Eco31I GGTCTC 1 cut(s) 141
EcoRII CCWGG 2 cut(s) 190, 366
Esp3I CGTCTC 1 cut(s) 115
FaeI CATG 1 cut(s) 463
FatI CATG 1 cut(s) 459
Fnu4HI GCNGC 6 cut(s) 36, 149, 240, 255, 379, 382
Fsp4HI GCNGC 6 cut(s) 36, 149, 240, 255, 379, 382
GlaI GCGC 2 cut(s) 198, 586
GluI GCNGC 6 cut(s) 36, 149, 240, 255, 379, 382
GsuI CTGGAG 3 cut(s) 24, 174, 389
HaeIII GGCC 2 cut(s) 292, 504
HhaI GCGC 2 cut(s) 199, 587
Hin1I GRCGYC 1 cut(s) 108
Hin1II CATG 1 cut(s) 463
Hin6I GCGC 2 cut(s) 197, 585
HinP1I GCGC 2 cut(s) 197, 585
HindIII AAGCTT 1 cut(s) 609
HinfI GANTC 2 cut(s) 463, 577
HphI GGTGA 1 cut(s) 424
Hpy166II GTNNAC 2 cut(s) 106, 499
Hpy188I TCNGA 1 cut(s) 298
Hpy188III TCNNGA 1 cut(s) 422
Hpy8I GTNNAC 2 cut(s) 106, 499
HpyAV CCTTC 3 cut(s) 126, 303, 314
HpyCH4III ACNGT 3 cut(s) 265, 519, 592
HpyCH4IV ACGT 1 cut(s) 108
HpyCH4V TGCA 6 cut(s) 35, 127, 242, 257, 360, 408
HpyF3I CTNAG 3 cut(s) 57, 204, 297
HpySE526I ACGT 1 cut(s) 108
Hsp92I GRCGYC 1 cut(s) 108
Hsp92II CATG 1 cut(s) 463
HspAI GCGC 2 cut(s) 197, 585
KpnI GGTACC 1 cut(s) 367
Kzo9I GATC 1 cut(s) 529
LmnI GCTCC 3 cut(s) 43, 323, 572
Lsp1109I GCAGC 5 cut(s) 47, 160, 226, 241, 368
MaeII ACGT 1 cut(s) 108
MaeIII GTNAC 1 cut(s) 603
MalI GATC 1 cut(s) 531
MboI GATC 1 cut(s) 529
MboII GAAGA 5 cut(s) 37, 265, 421, 488, 536
MflI RGATCY 1 cut(s) 529
MluCI AATT 3 cut(s) 215, 440, 469
MmeI TCCRAC 1 cut(s) 367
MnlI CCTC 7 cut(s) 21, 58, 160, 198, 292, 307, 520
MseI TTAA 2 cut(s) 260, 455
MspA1I CMGCKG 1 cut(s) 378
MspR9I CCNGG 2 cut(s) 192, 368
Mva1269I GAATGC 1 cut(s) 141
MvaI CCWGG 2 cut(s) 192, 368
NdeII GATC 1 cut(s) 529
NlaIII CATG 1 cut(s) 463
NlaIV GGNNCC 2 cut(s) 365, 531
PctI GAATGC 1 cut(s) 141
PfeI GAWTC 2 cut(s) 463, 577
PkrI GCNGC 6 cut(s) 37, 150, 241, 256, 380, 383
Psp6I CCWGG 2 cut(s) 190, 366
PspGI CCWGG 2 cut(s) 190, 366
PspN4I GGNNCC 2 cut(s) 365, 531
PspPI GGNCC 1 cut(s) 290
PstI CTGCAG 1 cut(s) 244
PstNI CAGNNNCTG 1 cut(s) 381
PsuI RGATCY 1 cut(s) 529
RsaI GTAC 2 cut(s) 365, 500
RsaNI GTAC 2 cut(s) 364, 499
SaqAI TTAA 2 cut(s) 260, 455
SatI GCNGC 6 cut(s) 36, 149, 240, 255, 379, 382
Sau3AI GATC 1 cut(s) 529
Sau96I GGNCC 1 cut(s) 290
ScrFI CCNGG 2 cut(s) 192, 368
SfcI CTRYAG 1 cut(s) 240
SmlI CTYRAG 1 cut(s) 420
SmoI CTYRAG 1 cut(s) 420
Sse9I AATT 3 cut(s) 215, 440, 469
SsiI CCGC 1 cut(s) 378
StyD4I CCNGG 2 cut(s) 190, 366
TaaI ACNGT 3 cut(s) 265, 519, 592
TaiI ACGT 1 cut(s) 111
TasI AATT 3 cut(s) 215, 440, 469
TauI GCSGC 1 cut(s) 381
TfiI GAWTC 2 cut(s) 463, 577
Tru1I TTAA 2 cut(s) 260, 455
Tru9I TTAA 2 cut(s) 260, 455
TscAI CASTG 4 cut(s) 48, 284, 540, 595
TseI GCWGC 5 cut(s) 35, 148, 239, 254, 381
TspDTI ATGAA 1 cut(s) 38
TspRI CASTG 4 cut(s) 48, 284, 540, 595
XapI RAATTY 2 cut(s) 440, 469
XcmI CCANNNNNNNNNTGG 2 cut(s) 231, 284
ZraI GACGTC 1 cut(s) 109
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.