Rw7G007490

Beta-carotene isomerase D27

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0016208
Physical Location & Seq
Forward (+)
6765053 .. 6767830
2778 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G007490.1

Sequence Viewer

Length: 795 bp
ATGGTGGTTTTAAGCCTTCAACCTGTCCAATTTACTACCCCTAAACAACTCTCATATTGTAAAACCAGCACCACTGGTTATAGTACTAGTGTTCGATGTGGGATTGCAGAGCCATCAGGAGTTCCAGCTCCTATGGGGCAAAAGACCAAATACAATGATGGATTTTTTGAGAAAGCATTCATGACACTCTTCGCTCGCAAAATGAAGAGGTTTGTAGCTCCAGTGAAATCCAAGACTGAGATTGAGGAAAAGAAATGGTGGGAATATGACTATGAGAGTTTTGTGGACGTATCCAAGAGAGTTATGCAGGGAAGGTCTCGCATTCAGCAGCAACAAGTGGTTAGGGAGGTCTTGTTATCTATGCTACCTCCAGGTGCGCCTGCTCAGTTTAGGAAATTATTTCCACCAACAAAGTGGGCTGCAGAGTTCAATGCTGCATTAACAGTGCCTTTCTTCCACTGGTTAGTTGGGCCTTCTGAGGTTATAGAAGTTGAGGTAGAAGGAGTGAAGCAAAGAAGTGGAGTTCGTATAAAGAAATGCAGGTACCTGGAGAACAGCGGCTGCGTTGGAATGTGTGTGAATATGTGCAAGTTTCCTACTCAAGATTTTTTCACCAATGAATTTGGGCTTCCATTAACCATGATTCCAAATTTTGAAGATATGAGTTGTGATATGGTGTACGGCCAAGTTCCACCACCGTTTGAAGAGGATCCAGTGTCCAAACAACCCTGTTTTACTGATATATGCTCCATTGCAAATCCCAGCGCCACTGTCTGTCCAAAGTTACAAGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4033

Protein Analysis

264

Amino Acids

29.84

Weight (kDa)

8.62

Isoelectric Point (pI)

51.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
D27-like_C PF13225 154 - 236 7e-38 Beta-carotene isomerase D27-like, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016208)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G64680 AT1G64680
fragaria_vesca FvH4_5g10820
malus_domestica MD14G1137300.v1.1
prunus_persica Prupe.5G131200_v2.0.a1
pyrus_communis pycom14g11240
rosa_chinensis RchiOBHm_Chr7g0187341
rosa_laevigata RLG00000004793
rosa_roxburghii Rroxscaffold_3G00267590
rosa_rugosa Rorug06G0480700
rosa_samantha Rh7AG085200 Rh7BG077400 Rh7CG086200 Rh7DG087300
rosa_wichuraiana Rw7G007490

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 531
Acc65I GGTACC 1 cut(s) 543
AccB1I GGYRCC 1 cut(s) 543
AciI CCGC 1 cut(s) 558
AclWI GGATC 2 cut(s) 704, 717
AcoI YGGCCR 1 cut(s) 682
AcsI RAATTY 2 cut(s) 620, 649
AfaI GTAC 3 cut(s) 85, 545, 680
AgsI TTSAA 4 cut(s) 20, 430, 656, 704
AhlI ACTAGT 1 cut(s) 86
AjnI CCWGG 2 cut(s) 370, 546
AluBI AGCT 3 cut(s) 128, 218, 791
AluI AGCT 3 cut(s) 128, 218, 791
Alw26I GTCTC 1 cut(s) 321
AlwI GGATC 2 cut(s) 704, 717
AlwNI CAGNNNCTG 1 cut(s) 561
AoxI GGCC 2 cut(s) 470, 682
ApeKI GCWGC 4 cut(s) 328, 419, 434, 561
ApoI RAATTY 2 cut(s) 620, 649
Asp700I GAANNNNTTC 1 cut(s) 176
Asp718I GGTACC 1 cut(s) 543
AspLEI GCGC 2 cut(s) 379, 767
AspS9I GGNCC 1 cut(s) 470
AsuHPI GGTGA 1 cut(s) 604
BamHI GGATCC 1 cut(s) 709
BanI GGYRCC 1 cut(s) 543
BbvI GCAGC 4 cut(s) 340, 406, 421, 548
BccI CCATC 2 cut(s) 121, 152
BceAI ACGGC 1 cut(s) 697
BciT130I CCWGG 2 cut(s) 372, 548
BciVI GTATCC 1 cut(s) 301
BcoDI GTCTC 1 cut(s) 321
BcuI ACTAGT 1 cut(s) 86
BfaI CTAG 1 cut(s) 87
BfmI CTRYAG 1 cut(s) 420
BfoI RGCGCY 1 cut(s) 768
BfuAI ACCTGC 1 cut(s) 531
BfuI GTATCC 1 cut(s) 301
BisI GCNGC 5 cut(s) 329, 420, 435, 559, 562
BlsI GCNGC 5 cut(s) 330, 421, 436, 560, 563
BmcAI AGTACT 1 cut(s) 85
Bme1390I CCNGG 2 cut(s) 372, 548
BmgT120I GGNCC 1 cut(s) 470
BmiI GGNNCC 2 cut(s) 545, 711
BmrFI CCNGG 2 cut(s) 372, 548
BpmI CTGGAG 3 cut(s) 204, 354, 569
BpuEI CTTGAG 1 cut(s) 585
BsaI GGTCTC 1 cut(s) 321
BsaXI ACNNNNNCTCC 2 cut(s) 338, 368
Bse1I ACTGG 4 cut(s) 79, 221, 464, 713
Bse3DI GCAATG 1 cut(s) 750
BseBI CCWGG 2 cut(s) 372, 548
BseMI GCAATG 1 cut(s) 750
BseMII CTCAG 3 cut(s) 228, 398, 468
BseNI ACTGG 4 cut(s) 79, 221, 464, 713
BseXI GCAGC 4 cut(s) 340, 406, 421, 548
BseYI CCCAGC 1 cut(s) 761
BshFI GGCC 2 cut(s) 472, 684
BshNI GGYRCC 1 cut(s) 543
BsmAI GTCTC 1 cut(s) 321
BsmI GAATGC 2 cut(s) 176, 321
BsnI GGCC 2 cut(s) 472, 684
Bso31I GGTCTC 1 cut(s) 321
Bsp143I GATC 1 cut(s) 709
BspACI CCGC 1 cut(s) 558
BspANI GGCC 2 cut(s) 472, 684
BspCNI CTCAG 3 cut(s) 229, 397, 469
BspHI TCATGA 1 cut(s) 180
BspLI GGNNCC 2 cut(s) 545, 711
BspMAI CTGCAG 1 cut(s) 424
BspMI ACCTGC 1 cut(s) 531
BspPI GGATC 2 cut(s) 704, 717
BspT107I GGYRCC 1 cut(s) 543
BspTNI GGTCTC 1 cut(s) 321
BsrDI GCAATG 1 cut(s) 750
BsrI ACTGG 4 cut(s) 79, 221, 464, 713
BssMI GATC 1 cut(s) 709
Bst2UI CCWGG 2 cut(s) 372, 548
Bst4CI ACNGT 3 cut(s) 445, 699, 772
Bst6I CTCTTC 3 cut(s) 194, 200, 699
BstC8I GCNNGC 2 cut(s) 196, 381
BstDEI CTNAG 3 cut(s) 237, 384, 477
BstH2I RGCGCY 1 cut(s) 768
BstHHI GCGC 2 cut(s) 379, 767
BstKTI GATC 1 cut(s) 712
BstMAI GTCTC 1 cut(s) 321
BstMBI GATC 1 cut(s) 709
BstNI CCWGG 2 cut(s) 372, 548
BstSCI CCNGG 2 cut(s) 370, 546
BstSFI CTRYAG 1 cut(s) 420
BstV1I GCAGC 4 cut(s) 340, 406, 421, 548
BstX2I RGATCY 1 cut(s) 709
BstXI CCANNNNNNTGG 1 cut(s) 414
BstYI RGATCY 1 cut(s) 709
BsuI GTATCC 1 cut(s) 301
BsuRI GGCC 2 cut(s) 472, 684
BtsIMutI CAGTG 6 cut(s) 72, 228, 450, 457, 720, 768
BveI ACCTGC 1 cut(s) 531
Cac8I GCNNGC 2 cut(s) 196, 381
CaiI CAGNNNCTG 1 cut(s) 561
CciI TCATGA 1 cut(s) 180
CfoI GCGC 2 cut(s) 379, 767
Cfr13I GGNCC 1 cut(s) 470
Csp6I GTAC 3 cut(s) 84, 544, 679
CviAII CATG 2 cut(s) 181, 640
CviQI GTAC 3 cut(s) 84, 544, 679
DdeI CTNAG 3 cut(s) 237, 384, 477
DpnI GATC 1 cut(s) 711
DpnII GATC 1 cut(s) 709
EaeI YGGCCR 1 cut(s) 682
Eam1104I CTCTTC 3 cut(s) 194, 200, 699
EarI CTCTTC 3 cut(s) 194, 200, 699
Eco31I GGTCTC 1 cut(s) 321
EcoRII CCWGG 2 cut(s) 370, 546
FaeI CATG 2 cut(s) 184, 643
FatI CATG 2 cut(s) 180, 639
Fnu4HI GCNGC 5 cut(s) 329, 420, 435, 559, 562
Fsp4HI GCNGC 5 cut(s) 329, 420, 435, 559, 562
FspBI CTAG 1 cut(s) 87
GlaI GCGC 2 cut(s) 378, 766
GluI GCNGC 5 cut(s) 329, 420, 435, 559, 562
GsaI CCCAGC 1 cut(s) 765
GsuI CTGGAG 3 cut(s) 204, 354, 569
HaeII RGCGCY 1 cut(s) 768
HaeIII GGCC 2 cut(s) 472, 684
HhaI GCGC 2 cut(s) 379, 767
Hin1II CATG 2 cut(s) 184, 643
Hin6I GCGC 2 cut(s) 377, 765
HinP1I GCGC 2 cut(s) 377, 765
HindIII AAGCTT 1 cut(s) 789
HinfI GANTC 1 cut(s) 643
HphI GGTGA 1 cut(s) 604
Hpy166II GTNNAC 2 cut(s) 286, 679
Hpy188I TCNGA 1 cut(s) 478
Hpy188III TCNNGA 3 cut(s) 117, 181, 602
Hpy8I GTNNAC 2 cut(s) 286, 679
HpyAV CCTTC 4 cut(s) 26, 306, 483, 494
HpyCH4III ACNGT 3 cut(s) 445, 699, 772
HpyCH4IV ACGT 1 cut(s) 288
HpyCH4V TGCA 7 cut(s) 107, 307, 422, 437, 540, 588, 755
HpyF3I CTNAG 3 cut(s) 237, 384, 477
HpySE526I ACGT 1 cut(s) 288
Hsp92II CATG 2 cut(s) 184, 643
HspAI GCGC 2 cut(s) 377, 765
KpnI GGTACC 1 cut(s) 547
Kzo9I GATC 1 cut(s) 709
LmnI GCTCC 3 cut(s) 133, 223, 752
Lsp1109I GCAGC 4 cut(s) 340, 406, 421, 548
MaeI CTAG 1 cut(s) 87
MaeII ACGT 1 cut(s) 288
MaeIII GTNAC 1 cut(s) 783
MalI GATC 1 cut(s) 711
MboI GATC 1 cut(s) 709
MboII GAAGA 5 cut(s) 181, 217, 445, 668, 716
MflI RGATCY 1 cut(s) 709
MluCI AATT 4 cut(s) 29, 395, 620, 649
MmeI TCCRAC 1 cut(s) 547
MnlI CCTC 7 cut(s) 201, 238, 340, 378, 472, 487, 700
MroXI GAANNNNTTC 1 cut(s) 176
MseI TTAA 3 cut(s) 11, 440, 635
MspA1I CMGCKG 1 cut(s) 558
MspR9I CCNGG 2 cut(s) 372, 548
Mva1269I GAATGC 2 cut(s) 176, 321
MvaI CCWGG 2 cut(s) 372, 548
NdeII GATC 1 cut(s) 709
NlaIII CATG 2 cut(s) 184, 643
NlaIV GGNNCC 2 cut(s) 545, 711
PagI TCATGA 1 cut(s) 180
PctI GAATGC 2 cut(s) 176, 321
PdmI GAANNNNTTC 1 cut(s) 176
PfeI GAWTC 1 cut(s) 643
PkrI GCNGC 5 cut(s) 330, 421, 436, 560, 563
Psp6I CCWGG 2 cut(s) 370, 546
PspFI CCCAGC 1 cut(s) 761
PspGI CCWGG 2 cut(s) 370, 546
PspN4I GGNNCC 2 cut(s) 545, 711
PspPI GGNCC 1 cut(s) 470
PstI CTGCAG 1 cut(s) 424
PstNI CAGNNNCTG 1 cut(s) 561
PsuI RGATCY 1 cut(s) 709
RsaI GTAC 3 cut(s) 85, 545, 680
RsaNI GTAC 3 cut(s) 84, 544, 679
SaqAI TTAA 3 cut(s) 11, 440, 635
SatI GCNGC 5 cut(s) 329, 420, 435, 559, 562
Sau3AI GATC 1 cut(s) 709
Sau96I GGNCC 1 cut(s) 470
ScaI AGTACT 1 cut(s) 85
ScrFI CCNGG 2 cut(s) 372, 548
SfcI CTRYAG 1 cut(s) 420
SmlI CTYRAG 1 cut(s) 600
SmoI CTYRAG 1 cut(s) 600
SpeI ACTAGT 1 cut(s) 86
Sse9I AATT 4 cut(s) 29, 395, 620, 649
SsiI CCGC 1 cut(s) 558
SspMI CTAG 1 cut(s) 87
StyD4I CCNGG 2 cut(s) 370, 546
TaaI ACNGT 3 cut(s) 445, 699, 772
TaiI ACGT 1 cut(s) 291
TaqI TCGA 1 cut(s) 94
TasI AATT 4 cut(s) 29, 395, 620, 649
TatI WGTACW 1 cut(s) 83
TauI GCSGC 1 cut(s) 561
TfiI GAWTC 1 cut(s) 643
Tru1I TTAA 3 cut(s) 11, 440, 635
Tru9I TTAA 3 cut(s) 11, 440, 635
TscAI CASTG 6 cut(s) 79, 228, 450, 464, 720, 775
TseI GCWGC 4 cut(s) 328, 419, 434, 561
TspDTI ATGAA 3 cut(s) 169, 218, 633
TspRI CASTG 6 cut(s) 79, 228, 450, 464, 720, 775
XapI RAATTY 2 cut(s) 620, 649
XcmI CCANNNNNNNNNTGG 2 cut(s) 411, 464
XmnI GAANNNNTTC 1 cut(s) 176
XspI CTAG 1 cut(s) 87
ZrmI AGTACT 1 cut(s) 85
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.