AT1G65230

Uncharacterized conserved protein (DUF2358)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0016218
Physical Location & Seq
Forward (+)
24229063 .. 24230902
1840 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G65230.1

Sequence Viewer

Length: 861 bp
ATGACTACTTCTTCCTTCCTCCTCCCTGCTTCTCCTCCCTCAGCCGCCTTTCTCCGGCGGCGCTTCTTCCGCCACTCCTGGCTTTCCTCTAAGATCCACCACCGTCTTCCCCAAGTTATGAATGATTCCACGAGGACTGAAGTATCCATTGATAAATCAGAAGTAGACAAGCTTGTTGACAAGATTGATTTTGGGGAATTATGCAACGATTTTGAGTGCACAAGTAGTCCGCAGGTGGAATCCACAGCTAGACAACTTGTTCGCGACATTCTTGAAATCAGAGAAGGAAATCGTGCCTTTGCATGCTATGCAGTTTCCGTTAAATATAAGGATCCGGTTAGAAGTTTTACAGGTCGTGAGAAGTACAAAAGACCAATGTGGATAACTAGTGGATTAGAGAATCCTACCGTGACGGTACAAGAGATGGTAATGTTATCGACCAGCGTCTTGCGAATTAAATGGACAGTGAAAGGAAAACCAAAATCTATTCTTGCTGCTGTAAGTGGAGATTTGATAGTAAAAGTCAAATCTGAATTTACTCTTAACCAGATTAGTGGGCAAGTGTTTGAGCATGAAGAGTCTTGGGACTTATCTTCATCATCCCCTATTGCTCAAGCCTATTTCTGGACATCCCGCCGCCTCTTTGCTGCTTCTGAATCCGCAAAAGATGTCGCCGATGTCACAAAAGACTTGACCGCCAATTTGACAACTCGAAAAGAGGACACAGATATATATCGCGATCCAACCGATCCAAATAAGTTTTTCCAAAGAGACAACAGCTTTGAGAGAGATGTGTATCAAATTGCACTGTTTCTAGCCATTGTCTATTTTGTTGTACAGTTCTTGAAGAACACAATCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2358

Protein Analysis

286

Amino Acids

32.74

Weight (kDa)

8.65

Isoelectric Point (pI)

40.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF2358 PF10184 95 - 191 2.1e-07 Uncharacterized conserved protein (DUF2358)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016218)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G65230
fragaria_vesca FvH4_6g00410
malus_domestica MD04G1245900.v1.1 MD12G1264100.v1.1
prunus_persica Prupe.6G362300_v2.0.a1
pyrus_communis pycom04g21680
rosa_chinensis RchiOBHm_Chr3g0447741
rosa_laevigata RLG00000025974
rosa_multiflora Rmu_ssc0000391.1_g000014
rosa_roxburghii Rroxscaffold_6G00425940
rosa_rugosa Rorug02G0605200 Rorug02G0605200
rosa_samantha Rh3AG004900 Rh3CG004900
rosa_wichuraiana Rw3G000360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 223
Acc36I ACCTGC 1 cut(s) 223
AccI GTMKAC 1 cut(s) 165
AccII CGCG 2 cut(s) 264, 738
AciI CCGC 8 cut(s) 45, 58, 70, 230, 634, 637, 660, 696
AclWI GGATC 5 cut(s) 88, 326, 339, 734, 743
AcsI RAATTY 1 cut(s) 533
AcuI CTGAAG 1 cut(s) 159
AfaI GTAC 3 cut(s) 365, 417, 837
AfiI CCNNNNNNNGG 2 cut(s) 54, 624
AgsI TTSAA 2 cut(s) 275, 847
AhlI ACTAGT 1 cut(s) 386
AjnI CCWGG 1 cut(s) 77
AluBI AGCT 3 cut(s) 172, 248, 780
AluI AGCT 3 cut(s) 172, 248, 780
Alw21I GWGCWC 1 cut(s) 221
Alw26I GTCTC 1 cut(s) 765
Alw44I GTGCAC 1 cut(s) 217
AlwI GGATC 5 cut(s) 88, 326, 339, 734, 743
ApaLI GTGCAC 1 cut(s) 217
ApeKI GCWGC 2 cut(s) 494, 647
ApoI RAATTY 1 cut(s) 533
ArsI GACNNNNNNTTYG 6 cut(s) 243, 275, 685, 717, 764, 796
AspLEI GCGC 1 cut(s) 63
BaeGI GKGCMC 1 cut(s) 221
BaeI ACNNNNGTAYC 2 cut(s) 126, 159
BamHI GGATCC 1 cut(s) 331
BauI CACGAG 1 cut(s) 130
BbsI GAAGAC 1 cut(s) 98
Bbv12I GWGCWC 1 cut(s) 221
BbvCI CCTCAGC 1 cut(s) 40
BbvI GCAGC 2 cut(s) 481, 634
BccI CCATC 1 cut(s) 418
BciT130I CCWGG 1 cut(s) 79
BciVI GTATCC 1 cut(s) 154
BcoDI GTCTC 1 cut(s) 765
BcuI ACTAGT 1 cut(s) 386
BfaI CTAG 4 cut(s) 249, 387, 815, 859
BfoI RGCGCY 1 cut(s) 64
BfuAI ACCTGC 1 cut(s) 223
BfuI GTATCC 1 cut(s) 154
BisI GCNGC 5 cut(s) 45, 59, 495, 637, 648
BlsI GCNGC 5 cut(s) 46, 60, 496, 638, 649
Bme1390I CCNGG 1 cut(s) 79
BmiI GGNNCC 1 cut(s) 333
BmrFI CCNGG 1 cut(s) 79
BoxI GACNNNNGTC 1 cut(s) 443
BpiI GAAGAC 1 cut(s) 98
Bpu10I CCTNAGC 1 cut(s) 40
BpuEI CTTGAG 1 cut(s) 597
BsaBI GATNNNNATC 2 cut(s) 732, 795
BsaWI WCCGGW 1 cut(s) 334
Bsc4I CCNNNNNNNGG 2 cut(s) 54, 624
Bse8I GATNNNNATC 2 cut(s) 732, 795
BseBI CCWGG 1 cut(s) 79
BseGI GGATG 2 cut(s) 599, 629
BseJI GATNNNNATC 2 cut(s) 732, 795
BseLI CCNNNNNNNGG 2 cut(s) 54, 624
BseMII CTCAG 1 cut(s) 54
BseRI GAGGAG 2 cut(s) 11, 24
BseSI GKGCMC 1 cut(s) 221
BseXI GCAGC 2 cut(s) 481, 634
Bsh1236I CGCG 2 cut(s) 264, 738
BsiHKAI GWGCWC 1 cut(s) 221
BsiSI CCGG 2 cut(s) 55, 335
BslFI GGGAC 1 cut(s) 599
BslI CCNNNNNNNGG 2 cut(s) 54, 624
BsmAI GTCTC 1 cut(s) 765
BsmFI GGGAC 1 cut(s) 599
Bsp1286I GDGCHC 1 cut(s) 221
Bsp1407I TGTACA 1 cut(s) 835
Bsp143I GATC 4 cut(s) 93, 331, 739, 748
Bsp68I TCGCGA 2 cut(s) 264, 738
BspACI CCGC 8 cut(s) 45, 58, 70, 230, 634, 637, 660, 696
BspCNI CTCAG 1 cut(s) 53
BspFNI CGCG 2 cut(s) 264, 738
BspLI GGNNCC 1 cut(s) 333
BspMI ACCTGC 1 cut(s) 223
BspPI GGATC 5 cut(s) 88, 326, 339, 734, 743
BsrGI TGTACA 1 cut(s) 835
BssMI GATC 4 cut(s) 93, 331, 739, 748
BssSI CACGAG 1 cut(s) 130
Bst2BI CACGAG 1 cut(s) 130
Bst2UI CCWGG 1 cut(s) 79
Bst4CI ACNGT 6 cut(s) 104, 409, 415, 466, 810, 840
Bst6I CTCTTC 1 cut(s) 570
BstAPI GCANNNNNTGC 1 cut(s) 308
BstAUI TGTACA 1 cut(s) 835
BstC8I GCNNGC 1 cut(s) 304
BstDEI CTNAG 2 cut(s) 40, 90
BstF5I GGATG 2 cut(s) 599, 629
BstFNI CGCG 2 cut(s) 264, 738
BstH2I RGCGCY 1 cut(s) 64
BstHHI GCGC 1 cut(s) 63
BstKTI GATC 4 cut(s) 96, 334, 742, 751
BstMAI GTCTC 1 cut(s) 765
BstMBI GATC 4 cut(s) 93, 331, 739, 748
BstMWI GCNNNNNNNGC 2 cut(s) 69, 308
BstNI CCWGG 1 cut(s) 79
BstNSI RCATGY 1 cut(s) 306
BstPAI GACNNNNGTC 1 cut(s) 443
BstSCI CCNGG 1 cut(s) 77
BstSLI GKGCMC 1 cut(s) 221
BstUI CGCG 2 cut(s) 264, 738
BstV1I GCAGC 2 cut(s) 481, 634
BstV2I GAAGAC 1 cut(s) 98
BstX2I RGATCY 2 cut(s) 93, 331
BstXI CCANNNNNNTGG 1 cut(s) 554
BstYI RGATCY 2 cut(s) 93, 331
BsuI GTATCC 1 cut(s) 154
BtsCI GGATG 2 cut(s) 599, 629
BtsIMutI CAGTG 2 cut(s) 471, 806
BtuMI TCGCGA 2 cut(s) 264, 738
BveI ACCTGC 1 cut(s) 223
Cac8I GCNNGC 1 cut(s) 304
CfoI GCGC 1 cut(s) 63
CseI GACGC 1 cut(s) 433
Csp6I GTAC 3 cut(s) 364, 416, 836
CviAII CATG 2 cut(s) 303, 572
CviJI RGCY 7 cut(s) 44, 82, 172, 248, 617, 780, 818
CviKI_1 RGCY 7 cut(s) 44, 82, 172, 248, 617, 780, 818
CviQI GTAC 3 cut(s) 364, 416, 836
DdeI CTNAG 2 cut(s) 40, 90
DpnI GATC 4 cut(s) 95, 333, 741, 750
DpnII GATC 4 cut(s) 93, 331, 739, 748
Eam1104I CTCTTC 1 cut(s) 570
EarI CTCTTC 1 cut(s) 570
EciI GGCGGA 1 cut(s) 59
Eco57I CTGAAG 1 cut(s) 159
EcoRII CCWGG 1 cut(s) 77
FaeI CATG 2 cut(s) 306, 575
FaiI YATR 8 cut(s) 119, 202, 304, 309, 327, 573, 731, 733
FaqI GGGAC 1 cut(s) 599
FatI CATG 2 cut(s) 302, 571
FauI CCCGC 1 cut(s) 641
FblI GTMKAC 1 cut(s) 165
Fnu4HI GCNGC 5 cut(s) 45, 59, 495, 637, 648
FokI GGATG 2 cut(s) 586, 616
Fsp4HI GCNGC 5 cut(s) 45, 59, 495, 637, 648
FspBI CTAG 4 cut(s) 249, 387, 815, 859
GlaI GCGC 1 cut(s) 62
GluI GCNGC 5 cut(s) 45, 59, 495, 637, 648
HaeII RGCGCY 1 cut(s) 64
HapII CCGG 2 cut(s) 55, 335
HgaI GACGC 1 cut(s) 433
HhaI GCGC 1 cut(s) 63
Hin1II CATG 2 cut(s) 306, 575
Hin6I GCGC 1 cut(s) 61
HinP1I GCGC 1 cut(s) 61
HincII GTYRAC 1 cut(s) 178
HindII GTYRAC 1 cut(s) 178
HindIII AAGCTT 1 cut(s) 170
HinfI GANTC 5 cut(s) 125, 239, 400, 578, 656
HpaII CCGG 2 cut(s) 55, 335
Hpy166II GTNNAC 3 cut(s) 166, 178, 219
Hpy188I TCNGA 4 cut(s) 160, 281, 532, 655
Hpy188III TCNNGA 6 cut(s) 263, 272, 356, 625, 737, 844
Hpy8I GTNNAC 3 cut(s) 166, 178, 219
HpyAV CCTTC 2 cut(s) 25, 278
HpyCH4III ACNGT 6 cut(s) 104, 409, 415, 466, 810, 840
HpyCH4V TGCA 5 cut(s) 204, 219, 302, 311, 806
HpyF10VI GCNNNNNNNGC 2 cut(s) 69, 308
HpyF3I CTNAG 2 cut(s) 40, 90
Hsp92II CATG 2 cut(s) 306, 575
HspAI GCGC 1 cut(s) 61
Kzo9I GATC 4 cut(s) 93, 331, 739, 748
Lsp1109I GCAGC 2 cut(s) 481, 634
MaeI CTAG 4 cut(s) 249, 387, 815, 859
MaeIII GTNAC 2 cut(s) 409, 679
MalI GATC 4 cut(s) 95, 333, 741, 750
MboI GATC 4 cut(s) 93, 331, 739, 748
MboII GAAGA 6 cut(s) 3, 58, 98, 585, 587, 859
MflI RGATCY 2 cut(s) 93, 331
MhlI GDGCHC 1 cut(s) 221
MluCI AATT 5 cut(s) 197, 453, 533, 700, 801
MlyI GAGTC 1 cut(s) 587
MmeI TCCRAC 1 cut(s) 767
MnlI CCTC 8 cut(s) 29, 32, 45, 49, 97, 126, 650, 712
MseI TTAA 3 cut(s) 321, 456, 543
MspI CCGG 2 cut(s) 55, 335
MspR9I CCNGG 1 cut(s) 79
MvaI CCWGG 1 cut(s) 79
MvnI CGCG 2 cut(s) 264, 738
MwoI GCNNNNNNNGC 2 cut(s) 69, 308
NdeII GATC 4 cut(s) 93, 331, 739, 748
NlaIII CATG 2 cut(s) 306, 575
NlaIV GGNNCC 1 cut(s) 333
NmuCI GTSAC 2 cut(s) 409, 679
NruI TCGCGA 2 cut(s) 264, 738
NspI RCATGY 1 cut(s) 306
PaeI GCATGC 1 cut(s) 306
PaqCI CACCTGC 1 cut(s) 223
PfeI GAWTC 4 cut(s) 125, 239, 400, 656
PkrI GCNGC 5 cut(s) 46, 60, 496, 638, 649
PleI GAGTC 1 cut(s) 586
PpsI GAGTC 1 cut(s) 586
PshAI GACNNNNGTC 1 cut(s) 443
Psp6I CCWGG 1 cut(s) 77
PspGI CCWGG 1 cut(s) 77
PspN4I GGNNCC 1 cut(s) 333
PsuI RGATCY 2 cut(s) 93, 331
RruI TCGCGA 2 cut(s) 264, 738
RsaI GTAC 3 cut(s) 365, 417, 837
RsaNI GTAC 3 cut(s) 364, 416, 836
SaqAI TTAA 3 cut(s) 321, 456, 543
SatI GCNGC 5 cut(s) 45, 59, 495, 637, 648
Sau3AI GATC 4 cut(s) 93, 331, 739, 748
SchI GAGTC 1 cut(s) 587
ScrFI CCNGG 1 cut(s) 79
SduI GDGCHC 1 cut(s) 221
SetI ASST 5 cut(s) 174, 237, 250, 355, 782
SmlI CTYRAG 1 cut(s) 612
SmoI CTYRAG 1 cut(s) 612
SpeI ACTAGT 1 cut(s) 386
SphI GCATGC 1 cut(s) 306
Sse9I AATT 5 cut(s) 197, 453, 533, 700, 801
SsiI CCGC 8 cut(s) 45, 58, 70, 230, 634, 637, 660, 696
SspMI CTAG 4 cut(s) 249, 387, 815, 859
StyD4I CCNGG 1 cut(s) 77
TaaI ACNGT 6 cut(s) 104, 409, 415, 466, 810, 840
TaqI TCGA 2 cut(s) 437, 712
TasI AATT 5 cut(s) 197, 453, 533, 700, 801
TatI WGTACW 2 cut(s) 363, 835
TauI GCSGC 3 cut(s) 47, 61, 639
TfiI GAWTC 4 cut(s) 125, 239, 400, 656
Tru1I TTAA 3 cut(s) 321, 456, 543
Tru9I TTAA 3 cut(s) 321, 456, 543
TscAI CASTG 2 cut(s) 471, 813
TseFI GTSAC 2 cut(s) 409, 679
TseI GCWGC 2 cut(s) 494, 647
Tsp45I GTSAC 2 cut(s) 409, 679
TspDTI ATGAA 3 cut(s) 134, 585, 588
TspGWI ACGGA 1 cut(s) 307
TspRI CASTG 2 cut(s) 471, 813
VneI GTGCAC 1 cut(s) 217
XapI RAATTY 1 cut(s) 533
XceI RCATGY 1 cut(s) 306
XmiI GTMKAC 1 cut(s) 165
XspI CTAG 4 cut(s) 249, 387, 815, 859
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.