MD04G1245900.v1.1

Uncharacterized conserved protein (DUF2358)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0016218
Physical Location & Seq
Forward (+)
32124908 .. 32127561
2654 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1245900.v1.1.491

Sequence Viewer

Length: 984 bp
ATGAGTGCCTCCGTTTCCATCACTGCCCTCATTGCCTCTAACTCGGCACCTGCCGCCGCCACCAACATCAATCTCCACCGCAACACTACTAGTTCCTCCACTACCACGACTACTAATGCAGCTAAATCAATAATTTTCACCTCCACCGCCAATTCCAAGCCCAATTTTACCGCCTCCTCTTCCTCCTCCTCCCAATACCACCACCCTTTCATACTGCGAGTAACAAATGATCCGGCTAGCAGTAGTAGTACTACTGAATCAGAATTGTCGGCGACGGATGAAAGAACAGTGGCTGATAGAATTGTTGATGGATTGAACTTTGGGGAGCTATGCAATGAGTTTGAGTGCATTAGTAGCCCCTTGGTGGAGTCTACAGCTCGACAACTTGTACGCGATATCCTTGAGCTACGAGAAGGCAATCGTGCCCTTGGAACCTTTGCTGTTTCTGTTAAATACAAGGATCCAGTTAGAAGTTTTAGCGGCCGTGATAAGTACAAGAGACCGCTATGGATAACCAGTGCACTAGAAAAGCCCTCTGTCAGTGTGCAAGAAATGAGCATGTTATCAACGAGTGTCCTGAGTATCAAGTGGACAATCAAAGGCAAGGCGAAGAACATTATAGGAGGAGATTTGATCGTAGGAATAAATTCCCAATTCACTCTAAACCAGATAAGTGGACAAGTCATTGAACATGAAGAGTTTTGGGATTTATCAGCTTCCTCAGCCATTGCGAAAGCGTATTTCTGGACATCACGCCGTCTTTATGCAACAATCGAGGGTGGAAAAGACTTGGCTGATCTTGCTAAAACCATTTCCAGCAAGTTCCCCAGAGAAAAACAGAACACAGAAATTTATCCAGACCCTTCAGGCGATCCAACTAAGTTCTTTCAAAGGGATGACGGTTTCCAAAGAGATGTATACCAAATTGGACTGTTTCTGGCACTGGTTTATTTTGTTGTACAGCTTTTGAGGACAACCTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2358

Protein Analysis

328

Amino Acids

35.9

Weight (kDa)

7.73

Isoelectric Point (pI)

41.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF2358 PF10184 146 - 231 8.8e-06 Uncharacterized conserved protein (DUF2358)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016218)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G65230
fragaria_vesca FvH4_6g00410
malus_domestica MD04G1245900.v1.1 MD12G1264100.v1.1
prunus_persica Prupe.6G362300_v2.0.a1
pyrus_communis pycom04g21680
rosa_chinensis RchiOBHm_Chr3g0447741
rosa_laevigata RLG00000025974
rosa_multiflora Rmu_ssc0000391.1_g000014
rosa_roxburghii Rroxscaffold_6G00425940
rosa_rugosa Rorug02G0605200 Rorug02G0605200
rosa_samantha Rh3AG004900 Rh3CG004900
rosa_wichuraiana Rw3G000360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 58
Acc36I ACCTGC 1 cut(s) 58
AccB1I GGYRCC 1 cut(s) 46
AccI GTMKAC 2 cut(s) 371, 918
AccII CGCG 1 cut(s) 393
AciI CCGC 7 cut(s) 54, 57, 79, 147, 171, 480, 503
AclWI GGATC 4 cut(s) 224, 455, 468, 866
AcoI YGGCCR 1 cut(s) 481
AcsI RAATTY 2 cut(s) 646, 849
AcuI CTGAAG 1 cut(s) 849
AfaI GTAC 4 cut(s) 250, 390, 494, 960
AfiI CCNNNNNNNGG 1 cut(s) 364
AgsI TTSAA 3 cut(s) 316, 689, 890
AhlI ACTAGT 1 cut(s) 89
AluBI AGCT 6 cut(s) 122, 328, 377, 406, 716, 964
AluI AGCT 6 cut(s) 122, 328, 377, 406, 716, 964
Alw21I GWGCWC 1 cut(s) 523
Alw26I GTCTC 1 cut(s) 493
Alw44I GTGCAC 1 cut(s) 519
AlwI GGATC 4 cut(s) 224, 455, 468, 866
AlwNI CAGNNNCTG 1 cut(s) 293
AoxI GGCC 1 cut(s) 481
ApaLI GTGCAC 1 cut(s) 519
ApeKI GCWGC 1 cut(s) 119
ApoI RAATTY 2 cut(s) 646, 849
Asp700I GAANNNNTTC 1 cut(s) 646
AsuHPI GGTGA 1 cut(s) 130
AsuNHI GCTAGC 1 cut(s) 236
BaeGI GKGCMC 2 cut(s) 427, 523
BamHI GGATCC 1 cut(s) 460
BanI GGYRCC 1 cut(s) 46
Bbv12I GWGCWC 1 cut(s) 523
BbvCI CCTCAGC 1 cut(s) 721
BbvI GCAGC 1 cut(s) 131
BccI CCATC 2 cut(s) 26, 302
BceAI ACGGC 2 cut(s) 468, 741
BcoDI GTCTC 1 cut(s) 493
BcuI ACTAGT 1 cut(s) 89
BfaI CTAG 3 cut(s) 90, 237, 524
BfmI CTRYAG 1 cut(s) 372
BfuAI ACCTGC 1 cut(s) 58
BisI GCNGC 4 cut(s) 54, 57, 120, 481
BlsI GCNGC 4 cut(s) 55, 58, 121, 482
BmcAI AGTACT 1 cut(s) 250
BmiI GGNNCC 3 cut(s) 48, 433, 462
BmtI GCTAGC 1 cut(s) 240
Bpu10I CCTNAGC 1 cut(s) 721
BpuEI CTTGAG 1 cut(s) 422
BsaI GGTCTC 1 cut(s) 493
BsaJI CCNNGG 2 cut(s) 360, 427
Bsc4I CCNNNNNNNGG 1 cut(s) 364
Bse1I ACTGG 3 cut(s) 464, 516, 948
Bse3DI GCAATG 3 cut(s) 30, 340, 726
BseDI CCNNGG 2 cut(s) 360, 427
BseGI GGATG 2 cut(s) 283, 901
BseLI CCNNNNNNNGG 1 cut(s) 364
BseMI GCAATG 3 cut(s) 30, 340, 726
BseMII CTCAG 2 cut(s) 569, 735
BseNI ACTGG 3 cut(s) 464, 516, 948
BseRI GAGGAG 4 cut(s) 166, 175, 178, 639
BseSI GKGCMC 2 cut(s) 427, 523
BseX3I CGGCCG 1 cut(s) 481
BseXI GCAGC 1 cut(s) 131
Bsh1236I CGCG 1 cut(s) 393
Bsh1285I CGRYCG 1 cut(s) 484
BshFI GGCC 1 cut(s) 483
BshNI GGYRCC 1 cut(s) 46
BsiEI CGRYCG 1 cut(s) 484
BsiHKAI GWGCWC 1 cut(s) 523
BsiSI CCGG 1 cut(s) 233
BslI CCNNNNNNNGG 1 cut(s) 364
BsmAI GTCTC 1 cut(s) 493
BsnI GGCC 1 cut(s) 483
Bso31I GGTCTC 1 cut(s) 493
Bsp1286I GDGCHC 2 cut(s) 427, 523
Bsp1407I TGTACA 1 cut(s) 958
Bsp143I GATC 5 cut(s) 229, 460, 633, 796, 871
BspACI CCGC 7 cut(s) 54, 57, 79, 147, 171, 480, 503
BspANI GGCC 1 cut(s) 483
BspCNI CTCAG 2 cut(s) 570, 734
BspFNI CGCG 1 cut(s) 393
BspLI GGNNCC 3 cut(s) 48, 433, 462
BspMI ACCTGC 1 cut(s) 58
BspOI GCTAGC 1 cut(s) 240
BspPI GGATC 4 cut(s) 224, 455, 468, 866
BspT107I GGYRCC 1 cut(s) 46
BspTNI GGTCTC 1 cut(s) 493
BsrDI GCAATG 3 cut(s) 30, 340, 726
BsrGI TGTACA 1 cut(s) 958
BsrI ACTGG 3 cut(s) 464, 516, 948
BssECI CCNNGG 2 cut(s) 360, 427
BssMI GATC 5 cut(s) 229, 460, 633, 796, 871
BssNAI GTATAC 1 cut(s) 919
BssT1I CCWWGG 2 cut(s) 360, 427
Bst1107I GTATAC 1 cut(s) 919
Bst4CI ACNGT 3 cut(s) 289, 902, 933
Bst6I CTCTTC 2 cut(s) 184, 690
BstAUI TGTACA 1 cut(s) 958
BstC8I GCNNGC 1 cut(s) 238
BstDEI CTNAG 3 cut(s) 578, 721, 879
BstF5I GGATG 2 cut(s) 283, 901
BstFNI CGCG 1 cut(s) 393
BstKTI GATC 5 cut(s) 232, 463, 636, 799, 874
BstMAI GTCTC 1 cut(s) 493
BstMBI GATC 5 cut(s) 229, 460, 633, 796, 871
BstMCI CGRYCG 1 cut(s) 484
BstMWI GCNNNNNNNGC 5 cut(s) 32, 53, 354, 722, 800
BstNSI RCATGY 1 cut(s) 562
BstSFI CTRYAG 1 cut(s) 372
BstSLI GKGCMC 2 cut(s) 427, 523
BstUI CGCG 1 cut(s) 393
BstV1I GCAGC 1 cut(s) 131
BstX2I RGATCY 1 cut(s) 460
BstXI CCANNNNNNTGG 1 cut(s) 674
BstYI RGATCY 1 cut(s) 460
BstZ17I GTATAC 1 cut(s) 919
BstZI CGGCCG 1 cut(s) 481
BsuRI GGCC 1 cut(s) 483
BtsCI GGATG 2 cut(s) 283, 901
BtsI GCAGTG 1 cut(s) 21
BtsIMutI CAGTG 5 cut(s) 21, 294, 523, 547, 941
BveI ACCTGC 1 cut(s) 58
Cac8I GCNNGC 1 cut(s) 238
CaiI CAGNNNCTG 1 cut(s) 293
Csp6I GTAC 4 cut(s) 249, 389, 493, 959
CviAII CATG 2 cut(s) 559, 692
CviQI GTAC 4 cut(s) 249, 389, 493, 959
DdeI CTNAG 3 cut(s) 578, 721, 879
DpnI GATC 5 cut(s) 231, 462, 635, 798, 873
DpnII GATC 5 cut(s) 229, 460, 633, 796, 871
EaeI YGGCCR 1 cut(s) 481
EagI CGGCCG 1 cut(s) 481
Eam1104I CTCTTC 2 cut(s) 184, 690
EarI CTCTTC 2 cut(s) 184, 690
EclXI CGGCCG 1 cut(s) 481
Eco130I CCWWGG 2 cut(s) 360, 427
Eco31I GGTCTC 1 cut(s) 493
Eco32I GATATC 1 cut(s) 397
Eco52I CGGCCG 1 cut(s) 481
Eco57I CTGAAG 1 cut(s) 849
EcoRV GATATC 1 cut(s) 397
EcoT14I CCWWGG 2 cut(s) 360, 427
ErhI CCWWGG 2 cut(s) 360, 427
FaeI CATG 2 cut(s) 562, 695
FaiI YATR 8 cut(s) 212, 331, 508, 560, 620, 693, 765, 919
FatI CATG 2 cut(s) 558, 691
FblI GTMKAC 2 cut(s) 371, 918
Fnu4HI GCNGC 4 cut(s) 54, 57, 120, 481
FokI GGATG 2 cut(s) 290, 908
Fsp4HI GCNGC 4 cut(s) 54, 57, 120, 481
FspBI CTAG 3 cut(s) 90, 237, 524
GluI GCNGC 4 cut(s) 54, 57, 120, 481
HaeIII GGCC 1 cut(s) 483
HapII CCGG 1 cut(s) 233
Hin1II CATG 2 cut(s) 562, 695
HinfI GANTC 2 cut(s) 257, 368
HpaII CCGG 1 cut(s) 233
HphI GGTGA 1 cut(s) 130
Hpy166II GTNNAC 5 cut(s) 372, 521, 591, 677, 919
Hpy188I TCNGA 1 cut(s) 262
Hpy188III TCNNGA 3 cut(s) 577, 745, 857
Hpy8I GTNNAC 5 cut(s) 372, 521, 591, 677, 919
Hpy99I CGWCG 1 cut(s) 277
HpyAV CCTTC 2 cut(s) 407, 873
HpyCH4III ACNGT 3 cut(s) 289, 902, 933
HpyCH4V TGCA 6 cut(s) 119, 333, 348, 521, 547, 767
HpyF10VI GCNNNNNNNGC 5 cut(s) 32, 53, 354, 722, 800
HpyF3I CTNAG 3 cut(s) 578, 721, 879
Hsp92II CATG 2 cut(s) 562, 695
Kzo9I GATC 5 cut(s) 229, 460, 633, 796, 871
LmnI GCTCC 1 cut(s) 325
Lsp1109I GCAGC 1 cut(s) 131
MaeI CTAG 3 cut(s) 90, 237, 524
MaeIII GTNAC 1 cut(s) 220
MalI GATC 5 cut(s) 231, 462, 635, 798, 873
MboI GATC 5 cut(s) 229, 460, 633, 796, 871
MboII GAAGA 3 cut(s) 171, 622, 707
MflI RGATCY 1 cut(s) 460
MhlI GDGCHC 2 cut(s) 427, 523
MluCI AATT 9 cut(s) 132, 151, 163, 263, 300, 646, 653, 849, 924
MlyI GAGTC 1 cut(s) 377
MmeI TCCRAC 1 cut(s) 899
MroXI GAANNNNTTC 1 cut(s) 646
MseI TTAA 1 cut(s) 450
MspI CCGG 1 cut(s) 233
MvnI CGCG 1 cut(s) 393
MwoI GCNNNNNNNGC 5 cut(s) 32, 53, 354, 722, 800
NdeII GATC 5 cut(s) 229, 460, 633, 796, 871
NheI GCTAGC 1 cut(s) 236
NlaIII CATG 2 cut(s) 562, 695
NlaIV GGNNCC 3 cut(s) 48, 433, 462
NmeAIII GCCGAG 1 cut(s) 23
NspI RCATGY 1 cut(s) 562
PaqCI CACCTGC 1 cut(s) 58
PdmI GAANNNNTTC 1 cut(s) 646
PfeI GAWTC 1 cut(s) 257
PkrI GCNGC 4 cut(s) 55, 58, 121, 482
PleI GAGTC 1 cut(s) 376
PpsI GAGTC 1 cut(s) 376
PspN4I GGNNCC 3 cut(s) 48, 433, 462
PstNI CAGNNNCTG 1 cut(s) 293
PsuI RGATCY 1 cut(s) 460
RsaI GTAC 4 cut(s) 250, 390, 494, 960
RsaNI GTAC 4 cut(s) 249, 389, 493, 959
SaqAI TTAA 1 cut(s) 450
SatI GCNGC 4 cut(s) 54, 57, 120, 481
Sau3AI GATC 5 cut(s) 229, 460, 633, 796, 871
ScaI AGTACT 1 cut(s) 250
SchI GAGTC 1 cut(s) 377
SduI GDGCHC 2 cut(s) 427, 523
SfcI CTRYAG 1 cut(s) 372
SmlI CTYRAG 1 cut(s) 401
SmoI CTYRAG 1 cut(s) 401
SpeI ACTAGT 1 cut(s) 89
Sse9I AATT 9 cut(s) 132, 151, 163, 263, 300, 646, 653, 849, 924
SsiI CCGC 7 cut(s) 54, 57, 79, 147, 171, 480, 503
SspMI CTAG 3 cut(s) 90, 237, 524
StyI CCWWGG 2 cut(s) 360, 427
TaaI ACNGT 3 cut(s) 289, 902, 933
TaqI TCGA 2 cut(s) 379, 774
TasI AATT 9 cut(s) 132, 151, 163, 263, 300, 646, 653, 849, 924
TatI WGTACW 3 cut(s) 248, 492, 958
TauI GCSGC 3 cut(s) 56, 59, 483
TfiI GAWTC 1 cut(s) 257
Tru1I TTAA 1 cut(s) 450
Tru9I TTAA 1 cut(s) 450
TscAI CASTG 5 cut(s) 28, 294, 523, 547, 948
TseI GCWGC 1 cut(s) 119
TspDTI ATGAA 3 cut(s) 199, 294, 708
TspGWI ACGGA 1 cut(s) 290
TspRI CASTG 5 cut(s) 28, 294, 523, 547, 948
VneI GTGCAC 1 cut(s) 519
XapI RAATTY 2 cut(s) 646, 849
XceI RCATGY 1 cut(s) 562
XmiI GTMKAC 2 cut(s) 371, 918
XmnI GAANNNNTTC 1 cut(s) 646
XspI CTAG 3 cut(s) 90, 237, 524
ZrmI AGTACT 1 cut(s) 250
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.