pycom04g21680

Uncharacterized conserved protein (DUF2358)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0016218
Physical Location & Seq
Forward (+)
22719023 .. 22721353
2331 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g21680.1

Sequence Viewer

Length: 960 bp
ATGAGTGCCTCCGTTTCCATCACTGCCCTCATTGCCTCTAACTCCGCCGCCGCTGCCGCCACCACCAGCAACCTCCACCGCAAAACTACTAATGCAGCTAAATCAACAATTTTCACCTCCACCTCCAATACCAAGCCTCATTTTACTGCCTCCTCTTCCTCCTCCTCCCATTACCACCATCCTTTCATACTGCGAGTGACAAATGATCCGACTAGCAGTAGTAGTACTAGTGAATCGGAATTGTCAGCGACGGATGAAAGAACAGTGGCTGATAGAATTGTTGATGGATTGAACTTTGGGGAGCTATGCAATGAGTTTGAGTGCATTAGTAGCCCCTTGGTGGAGTCTACAGCTCGACAACTTGCACGCGATATCCTTGAGCTACGAGAAGGCAATCGCGCCCTTGGAACCTTCGCTGTTTCTGTTAAATACAAGGATCCAGTTCGAAGTTTTAGCGGCCGTGATAAGTACAAGAGACCGCTATGGATAACCAGTGCACTAGACAAGCCCTCTGTCAGTGTGCAAGAAATGAGCATGTTATCAACGAGTGTCCTAAGTATCAAGTGGACGATCAAAGGGAAGGCGAAGAACATTATAGGAGGAGACTTGATCGTAAGAATAAATTCCCAGTTCACTCTAAACCAGATAAGTGGACAAGTCATTGAACATGAAGAGTTTTGGGATTTATCAGCTTCCTCGGCCATTGCGAAAGCATATTTCTGGACATCACGCCGTCTTTATGCAACAATCGAGGGTGGAAAAGACTTGGCTGATCTTGCTAAAACCATTTCCAGTAAGTTCCCCAGAGAAAAACAGAATACAGAAATTTATCCAGACCCTTCAGGCGATCCAACTAAGTTCTTTCAAAGGGATGACGGTTTCCAGAGAGATGTATACCAAATTGGACTGTTTCTGGCACTGGTTTATTTTGTTGTACAGCTTTTGAGGACAACCTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2358

Protein Analysis

320

Amino Acids

35.2

Weight (kDa)

8.73

Isoelectric Point (pI)

39.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF2358 PF10184 135 - 223 4.8e-06 Uncharacterized conserved protein (DUF2358)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016218)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G65230
fragaria_vesca FvH4_6g00410
malus_domestica MD04G1245900.v1.1 MD12G1264100.v1.1
prunus_persica Prupe.6G362300_v2.0.a1
pyrus_communis pycom04g21680
rosa_chinensis RchiOBHm_Chr3g0447741
rosa_laevigata RLG00000025974
rosa_multiflora Rmu_ssc0000391.1_g000014
rosa_roxburghii Rroxscaffold_6G00425940
rosa_rugosa Rorug02G0605200 Rorug02G0605200
rosa_samantha Rh3AG004900 Rh3CG004900
rosa_wichuraiana Rw3G000360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 347, 894
AccII CGCG 2 cut(s) 369, 399
AciI CCGC 7 cut(s) 45, 48, 51, 57, 79, 456, 479
AclWI GGATC 4 cut(s) 200, 431, 444, 842
AcoI YGGCCR 2 cut(s) 457, 699
AcsI RAATTY 2 cut(s) 622, 825
AcuI CTGAAG 1 cut(s) 825
AfaI GTAC 3 cut(s) 226, 470, 936
AfiI CCNNNNNNNGG 1 cut(s) 340
AgsI TTSAA 3 cut(s) 292, 665, 866
AhlI ACTAGT 1 cut(s) 227
AluBI AGCT 6 cut(s) 98, 304, 353, 382, 692, 940
AluI AGCT 6 cut(s) 98, 304, 353, 382, 692, 940
Alw21I GWGCWC 1 cut(s) 499
Alw26I GTCTC 2 cut(s) 469, 597
Alw44I GTGCAC 1 cut(s) 495
AlwI GGATC 4 cut(s) 200, 431, 444, 842
AlwNI CAGNNNCTG 1 cut(s) 269
AoxI GGCC 2 cut(s) 457, 699
ApaLI GTGCAC 1 cut(s) 495
ApeKI GCWGC 2 cut(s) 53, 95
ApoI RAATTY 2 cut(s) 622, 825
Asp700I GAANNNNTTC 1 cut(s) 622
AspLEI GCGC 1 cut(s) 401
AsuHPI GGTGA 1 cut(s) 106
AsuII TTCGAA 1 cut(s) 445
BaeGI GKGCMC 1 cut(s) 499
BamHI GGATCC 1 cut(s) 436
Bbv12I GWGCWC 1 cut(s) 499
BbvI GCAGC 2 cut(s) 40, 107
BccI CCATC 3 cut(s) 26, 186, 278
BceAI ACGGC 2 cut(s) 444, 717
BcoDI GTCTC 2 cut(s) 469, 597
BcuI ACTAGT 1 cut(s) 227
BfaI CTAG 3 cut(s) 213, 228, 500
BfmI CTRYAG 1 cut(s) 348
BisI GCNGC 6 cut(s) 48, 51, 54, 57, 96, 457
BlsI GCNGC 6 cut(s) 49, 52, 55, 58, 97, 458
BmcAI AGTACT 1 cut(s) 226
BmiI GGNNCC 2 cut(s) 409, 438
BmrI ACTGGG 1 cut(s) 622
BmuI ACTGGG 1 cut(s) 622
Bpu14I TTCGAA 1 cut(s) 445
BpuEI CTTGAG 1 cut(s) 398
BsaI GGTCTC 1 cut(s) 469
BsaJI CCNNGG 3 cut(s) 336, 403, 696
Bsc4I CCNNNNNNNGG 1 cut(s) 340
Bse1I ACTGG 5 cut(s) 440, 492, 628, 792, 924
Bse3DI GCAATG 3 cut(s) 30, 316, 702
BseDI CCNNGG 3 cut(s) 336, 403, 696
BseGI GGATG 3 cut(s) 178, 259, 877
BseLI CCNNNNNNNGG 1 cut(s) 340
BseMI GCAATG 3 cut(s) 30, 316, 702
BseNI ACTGG 5 cut(s) 440, 492, 628, 792, 924
BseRI GAGGAG 4 cut(s) 142, 151, 154, 615
BseSI GKGCMC 1 cut(s) 499
BseX3I CGGCCG 1 cut(s) 457
BseXI GCAGC 2 cut(s) 40, 107
Bsh1236I CGCG 2 cut(s) 369, 399
Bsh1285I CGRYCG 1 cut(s) 460
BshFI GGCC 2 cut(s) 459, 701
BsiEI CGRYCG 1 cut(s) 460
BsiHKAI GWGCWC 1 cut(s) 499
BslI CCNNNNNNNGG 1 cut(s) 340
BsmAI GTCTC 2 cut(s) 469, 597
BsnI GGCC 2 cut(s) 459, 701
Bso31I GGTCTC 1 cut(s) 469
Bsp119I TTCGAA 1 cut(s) 445
Bsp1286I GDGCHC 1 cut(s) 499
Bsp1407I TGTACA 1 cut(s) 934
Bsp143I GATC 6 cut(s) 205, 436, 570, 609, 772, 847
BspACI CCGC 7 cut(s) 45, 48, 51, 57, 79, 456, 479
BspANI GGCC 2 cut(s) 459, 701
BspFNI CGCG 2 cut(s) 369, 399
BspLI GGNNCC 2 cut(s) 409, 438
BspPI GGATC 4 cut(s) 200, 431, 444, 842
BspT104I TTCGAA 1 cut(s) 445
BspTNI GGTCTC 1 cut(s) 469
BsrDI GCAATG 3 cut(s) 30, 316, 702
BsrGI TGTACA 1 cut(s) 934
BsrI ACTGG 5 cut(s) 440, 492, 628, 792, 924
BssECI CCNNGG 3 cut(s) 336, 403, 696
BssMI GATC 6 cut(s) 205, 436, 570, 609, 772, 847
BssNAI GTATAC 1 cut(s) 895
BssT1I CCWWGG 2 cut(s) 336, 403
Bst1107I GTATAC 1 cut(s) 895
Bst4CI ACNGT 3 cut(s) 265, 878, 909
Bst6I CTCTTC 2 cut(s) 160, 666
BstAUI TGTACA 1 cut(s) 934
BstBI TTCGAA 1 cut(s) 445
BstC8I GCNNGC 1 cut(s) 367
BstDEI CTNAG 2 cut(s) 554, 855
BstF5I GGATG 3 cut(s) 178, 259, 877
BstFNI CGCG 2 cut(s) 369, 399
BstHHI GCGC 1 cut(s) 401
BstKTI GATC 6 cut(s) 208, 439, 573, 612, 775, 850
BstMAI GTCTC 2 cut(s) 469, 597
BstMBI GATC 6 cut(s) 205, 436, 570, 609, 772, 847
BstMCI CGRYCG 1 cut(s) 460
BstMWI GCNNNNNNNGC 6 cut(s) 32, 53, 56, 330, 698, 776
BstNSI RCATGY 1 cut(s) 538
BstSFI CTRYAG 1 cut(s) 348
BstSLI GKGCMC 1 cut(s) 499
BstUI CGCG 2 cut(s) 369, 399
BstV1I GCAGC 2 cut(s) 40, 107
BstX2I RGATCY 1 cut(s) 436
BstXI CCANNNNNNTGG 1 cut(s) 650
BstYI RGATCY 1 cut(s) 436
BstZ17I GTATAC 1 cut(s) 895
BstZI CGGCCG 1 cut(s) 457
BsuRI GGCC 2 cut(s) 459, 701
BtsCI GGATG 3 cut(s) 178, 259, 877
BtsI GCAGTG 1 cut(s) 21
BtsIMutI CAGTG 5 cut(s) 21, 270, 499, 523, 917
Cac8I GCNNGC 1 cut(s) 367
CaiI CAGNNNCTG 1 cut(s) 269
CfoI GCGC 1 cut(s) 401
Csp6I GTAC 3 cut(s) 225, 469, 935
CviAII CATG 2 cut(s) 535, 668
CviQI GTAC 3 cut(s) 225, 469, 935
DdeI CTNAG 2 cut(s) 554, 855
DpnI GATC 6 cut(s) 207, 438, 572, 611, 774, 849
DpnII GATC 6 cut(s) 205, 436, 570, 609, 772, 847
EaeI YGGCCR 2 cut(s) 457, 699
EagI CGGCCG 1 cut(s) 457
Eam1104I CTCTTC 2 cut(s) 160, 666
EarI CTCTTC 2 cut(s) 160, 666
EciI GGCGGA 1 cut(s) 34
EclXI CGGCCG 1 cut(s) 457
Eco130I CCWWGG 2 cut(s) 336, 403
Eco31I GGTCTC 1 cut(s) 469
Eco32I GATATC 1 cut(s) 373
Eco52I CGGCCG 1 cut(s) 457
Eco57I CTGAAG 1 cut(s) 825
EcoRV GATATC 1 cut(s) 373
EcoT14I CCWWGG 2 cut(s) 336, 403
ErhI CCWWGG 2 cut(s) 336, 403
FaeI CATG 2 cut(s) 538, 671
FaiI YATR 9 cut(s) 188, 307, 484, 536, 596, 669, 715, 741, 895
FatI CATG 2 cut(s) 534, 667
FblI GTMKAC 2 cut(s) 347, 894
Fnu4HI GCNGC 6 cut(s) 48, 51, 54, 57, 96, 457
FokI GGATG 3 cut(s) 165, 266, 884
Fsp4HI GCNGC 6 cut(s) 48, 51, 54, 57, 96, 457
FspBI CTAG 3 cut(s) 213, 228, 500
GlaI GCGC 1 cut(s) 400
GluI GCNGC 6 cut(s) 48, 51, 54, 57, 96, 457
HaeIII GGCC 2 cut(s) 459, 701
HhaI GCGC 1 cut(s) 401
Hin1II CATG 2 cut(s) 538, 671
Hin6I GCGC 1 cut(s) 399
HinP1I GCGC 1 cut(s) 399
HinfI GANTC 2 cut(s) 233, 344
HphI GGTGA 1 cut(s) 106
Hpy166II GTNNAC 6 cut(s) 348, 497, 567, 633, 653, 895
Hpy188I TCNGA 2 cut(s) 210, 238
Hpy188III TCNNGA 3 cut(s) 721, 833, 883
Hpy8I GTNNAC 6 cut(s) 348, 497, 567, 633, 653, 895
Hpy99I CGWCG 1 cut(s) 253
HpyAV CCTTC 4 cut(s) 383, 421, 574, 849
HpyCH4III ACNGT 3 cut(s) 265, 878, 909
HpyCH4V TGCA 7 cut(s) 95, 309, 324, 365, 497, 523, 743
HpyF10VI GCNNNNNNNGC 6 cut(s) 32, 53, 56, 330, 698, 776
HpyF3I CTNAG 2 cut(s) 554, 855
Hsp92II CATG 2 cut(s) 538, 671
HspAI GCGC 1 cut(s) 399
Kzo9I GATC 6 cut(s) 205, 436, 570, 609, 772, 847
LmnI GCTCC 1 cut(s) 301
Lsp1109I GCAGC 2 cut(s) 40, 107
MaeI CTAG 3 cut(s) 213, 228, 500
MaeIII GTNAC 1 cut(s) 196
MalI GATC 6 cut(s) 207, 438, 572, 611, 774, 849
MboI GATC 6 cut(s) 205, 436, 570, 609, 772, 847
MboII GAAGA 3 cut(s) 147, 598, 683
MflI RGATCY 1 cut(s) 436
MhlI GDGCHC 1 cut(s) 499
MluCI AATT 6 cut(s) 108, 239, 276, 622, 825, 900
MlyI GAGTC 1 cut(s) 353
MmeI TCCRAC 2 cut(s) 233, 875
MroXI GAANNNNTTC 1 cut(s) 622
MseI TTAA 1 cut(s) 426
MspA1I CMGCKG 1 cut(s) 53
MvnI CGCG 2 cut(s) 369, 399
MwoI GCNNNNNNNGC 6 cut(s) 32, 53, 56, 330, 698, 776
NdeII GATC 6 cut(s) 205, 436, 570, 609, 772, 847
NlaIII CATG 2 cut(s) 538, 671
NlaIV GGNNCC 2 cut(s) 409, 438
NmeAIII GCCGAG 1 cut(s) 677
NmuCI GTSAC 1 cut(s) 196
NspI RCATGY 1 cut(s) 538
NspV TTCGAA 1 cut(s) 445
PcsI WCGNNNNNNNCGW 1 cut(s) 704
PdmI GAANNNNTTC 1 cut(s) 622
PfeI GAWTC 1 cut(s) 233
PkrI GCNGC 6 cut(s) 49, 52, 55, 58, 97, 458
PleI GAGTC 1 cut(s) 352
PpsI GAGTC 1 cut(s) 352
PspN4I GGNNCC 2 cut(s) 409, 438
PstNI CAGNNNCTG 1 cut(s) 269
PsuI RGATCY 1 cut(s) 436
RsaI GTAC 3 cut(s) 226, 470, 936
RsaNI GTAC 3 cut(s) 225, 469, 935
SaqAI TTAA 1 cut(s) 426
SatI GCNGC 6 cut(s) 48, 51, 54, 57, 96, 457
Sau3AI GATC 6 cut(s) 205, 436, 570, 609, 772, 847
ScaI AGTACT 1 cut(s) 226
SchI GAGTC 1 cut(s) 353
SduI GDGCHC 1 cut(s) 499
SfcI CTRYAG 1 cut(s) 348
SfuI TTCGAA 1 cut(s) 445
SmlI CTYRAG 1 cut(s) 377
SmoI CTYRAG 1 cut(s) 377
SpeI ACTAGT 1 cut(s) 227
Sse9I AATT 6 cut(s) 108, 239, 276, 622, 825, 900
SsiI CCGC 7 cut(s) 45, 48, 51, 57, 79, 456, 479
SspMI CTAG 3 cut(s) 213, 228, 500
StyI CCWWGG 2 cut(s) 336, 403
TaaI ACNGT 3 cut(s) 265, 878, 909
TaqI TCGA 3 cut(s) 355, 445, 750
TasI AATT 6 cut(s) 108, 239, 276, 622, 825, 900
TatI WGTACW 3 cut(s) 224, 468, 934
TauI GCSGC 4 cut(s) 50, 53, 59, 459
TfiI GAWTC 1 cut(s) 233
Tru1I TTAA 1 cut(s) 426
Tru9I TTAA 1 cut(s) 426
TscAI CASTG 5 cut(s) 28, 270, 499, 523, 924
TseFI GTSAC 1 cut(s) 196
TseI GCWGC 2 cut(s) 53, 95
Tsp45I GTSAC 1 cut(s) 196
TspDTI ATGAA 3 cut(s) 175, 270, 684
TspGWI ACGGA 1 cut(s) 266
TspRI CASTG 5 cut(s) 28, 270, 499, 523, 924
VneI GTGCAC 1 cut(s) 495
XapI RAATTY 2 cut(s) 622, 825
XceI RCATGY 1 cut(s) 538
XmiI GTMKAC 2 cut(s) 347, 894
XmnI GAANNNNTTC 1 cut(s) 622
XspI CTAG 3 cut(s) 213, 228, 500
ZrmI AGTACT 1 cut(s) 226
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.