RLG00000025974

Uncharacterized conserved protein (DUF2358)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0016218
Physical Location & Seq
Forward (+)
49583571 .. 49584959
1389 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000025974

Sequence Viewer

Length: 1008 bp
ATGATGATGATCATGATGATGAATAGCTGCTGCACTACTTGTACTAGTGCTACTACTTTCACTGCGCTCCTTGCAGCAACAACCACCACCACCAGCTGCTGCCTTCGCCGCCGTGAAAGCAAAACTACTACTGCTGCTAATAGTAAATCTATATTTTTCGTCTCCAAGTACTCCAAGCCTTTTAGTACTACTGCGTCTTCCTCCCAAGATTCACACCACGTCTACCACCCTTTTTTACTGCGCGTAACAAATGATCAGGGTGGGACTGAATTGTCCTCCTCCTCTGACTCTGATAAAGAATATCTAAGATCAGAGGCTGATAGAATTGTGGATGGCTTGAACTTTGGGGAGCTTTGCGACGAGTTTGAGTGCATTAGCAGCCCCTTGGTGGAGGCCACTGCTCGACAGCTTGTCCGCGACATCCTTGAGCTTCGGGAAGGCAATCGCGCCCTTGGAACCTTTGCTCTTTCGGTTCAATACAAGGACCCCGTCAGAAGTTTTAGTGGTCGTGACAAGTACAAGAGACCACTATGGATAACCACTGCACTAGAGAAGCCTTCTGTGAGTGTTCAAGAAATGAGTATGTTATCGACCAGTGTGCTTAGTATAAAATGGACAATCAAAGGCAAGCCCAAGAACATTCTAGGAGGAGATTTGATTCTAAAAATCAACTCCCAATTCACTCTGAATCAGATCAGTGGCCAAGTGACTCGACATGAAGAGTTTTGGGATTTATCAGCTTGTTCATTCATTTCACAAGCCTATTTCTGGACCTCGCGTCGTCTGTTTGCAACAATTGAGGGTGGAAAAGACTTGACTGATGCAGTTAAAAGTCTTTCCAGCCGCTTCCCAACAGAAAAACAGAACATGGACATTTTTCCAGACCCTTATGGTGATCCAACTAAGTTCTTTCAAAGGGACGACGGTTTCCAAAGAGATGTGTATCAAATTGCTCTGTTCCTGGCACTTGTTTATTTTGTTGTACAACTTTTGAGAACAACCTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2358

Protein Analysis

336

Amino Acids

37.73

Weight (kDa)

8.05

Isoelectric Point (pI)

47.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF2358 PF10184 154 - 239 4.2e-06 Uncharacterized conserved protein (DUF2358)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016218)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G65230
fragaria_vesca FvH4_6g00410
malus_domestica MD04G1245900.v1.1 MD12G1264100.v1.1
prunus_persica Prupe.6G362300_v2.0.a1
pyrus_communis pycom04g21680
rosa_chinensis RchiOBHm_Chr3g0447741
rosa_laevigata RLG00000025974
rosa_multiflora Rmu_ssc0000391.1_g000014
rosa_roxburghii Rroxscaffold_6G00425940
rosa_rugosa Rorug02G0605200 Rorug02G0605200
rosa_samantha Rh3AG004900 Rh3CG004900
rosa_wichuraiana Rw3G000360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 271
AccI GTMKAC 1 cut(s) 222
AccII CGCG 4 cut(s) 243, 417, 447, 778
AciI CCGC 3 cut(s) 109, 415, 844
AclWI GGATC 1 cut(s) 890
AcoI YGGCCR 1 cut(s) 700
AfaI GTAC 5 cut(s) 43, 170, 187, 518, 984
AfiI CCNNNNNNNGG 2 cut(s) 388, 768
AgsI TTSAA 4 cut(s) 340, 476, 572, 914
AhdI GACNNNNNGTC 2 cut(s) 410, 777
AhlI ACTAGT 1 cut(s) 44
AjiI CACGTC 1 cut(s) 220
AjnI CCWGG 1 cut(s) 960
AluBI AGCT 6 cut(s) 27, 96, 352, 409, 430, 740
AluI AGCT 6 cut(s) 27, 96, 352, 409, 430, 740
Alw26I GTCTC 2 cut(s) 166, 517
AlwI GGATC 1 cut(s) 890
AlwNI CAGNNNCTG 2 cut(s) 99, 317
AoxI GGCC 2 cut(s) 393, 700
ApeKI GCWGC 7 cut(s) 27, 30, 74, 96, 99, 134, 378
AspLEI GCGC 3 cut(s) 67, 243, 449
AspS9I GGNCC 2 cut(s) 484, 771
AsuHPI GGTGA 1 cut(s) 905
AvaII GGWCC 2 cut(s) 484, 771
BalI TGGCCA 1 cut(s) 702
BbsI GAAGAC 1 cut(s) 189
BbvI GCAGC 7 cut(s) 14, 17, 83, 86, 86, 121, 390
BccI CCATC 1 cut(s) 326
BceAI ACGGC 1 cut(s) 96
BcgI CGANNNNNNTGC 2 cut(s) 580, 614
BciT130I CCWGG 1 cut(s) 962
BclI TGATCA 2 cut(s) 9, 253
BcoDI GTCTC 2 cut(s) 166, 517
BcuI ACTAGT 1 cut(s) 44
BfaI CTAG 3 cut(s) 45, 548, 644
BisI GCNGC 9 cut(s) 28, 31, 75, 97, 100, 109, 135, 379, 844
BlsI GCNGC 9 cut(s) 29, 32, 76, 98, 101, 110, 136, 380, 845
BmcAI AGTACT 2 cut(s) 170, 187
Bme1390I CCNGG 1 cut(s) 962
Bme18I GGWCC 2 cut(s) 484, 771
BmeRI GACNNNNNGTC 2 cut(s) 410, 777
BmgBI CACGTC 1 cut(s) 220
BmgT120I GGNCC 2 cut(s) 484, 771
BmiI GGNNCC 2 cut(s) 457, 486
BmrFI CCNGG 1 cut(s) 962
BmsI GCATC 1 cut(s) 811
BpiI GAAGAC 1 cut(s) 189
BpuEI CTTGAG 1 cut(s) 446
BsaBI GATNNNNATC 2 cut(s) 8, 942
BsaI GGTCTC 1 cut(s) 517
BsaJI CCNNGG 2 cut(s) 384, 451
Bsc4I CCNNNNNNNGG 2 cut(s) 388, 768
Bse1I ACTGG 1 cut(s) 594
Bse8I GATNNNNATC 2 cut(s) 8, 942
BseBI CCWGG 1 cut(s) 962
BseDI CCNNGG 2 cut(s) 384, 451
BseGI GGATG 2 cut(s) 337, 420
BseJI GATNNNNATC 2 cut(s) 8, 942
BseLI CCNNNNNNNGG 2 cut(s) 388, 768
BseNI ACTGG 1 cut(s) 594
BseRI GAGGAG 3 cut(s) 268, 271, 663
BseXI GCAGC 7 cut(s) 14, 17, 83, 86, 86, 121, 390
BsgI GTGCAG 2 cut(s) 16, 528
Bsh1236I CGCG 4 cut(s) 243, 417, 447, 778
BshFI GGCC 2 cut(s) 395, 702
BslFI GGGAC 2 cut(s) 277, 932
BslI CCNNNNNNNGG 2 cut(s) 388, 768
BsmAI GTCTC 2 cut(s) 166, 517
BsmBI CGTCTC 1 cut(s) 166
BsmFI GGGAC 2 cut(s) 277, 932
BsnI GGCC 2 cut(s) 395, 702
Bso31I GGTCTC 1 cut(s) 517
Bsp1407I TGTACA 1 cut(s) 982
Bsp143I GATC 5 cut(s) 9, 253, 308, 693, 895
BspACI CCGC 3 cut(s) 109, 415, 844
BspANI GGCC 2 cut(s) 395, 702
BspFNI CGCG 4 cut(s) 243, 417, 447, 778
BspHI TCATGA 1 cut(s) 12
BspLI GGNNCC 2 cut(s) 457, 486
BspPI GGATC 1 cut(s) 890
BspTNI GGTCTC 1 cut(s) 517
BsrGI TGTACA 1 cut(s) 982
BsrI ACTGG 1 cut(s) 594
BssECI CCNNGG 2 cut(s) 384, 451
BssMI GATC 5 cut(s) 9, 253, 308, 693, 895
BssT1I CCWWGG 2 cut(s) 384, 451
Bst2UI CCWGG 1 cut(s) 962
Bst4CI ACNGT 1 cut(s) 926
Bst6I CTCTTC 1 cut(s) 714
BstAUI TGTACA 1 cut(s) 982
BstC8I GCNNGC 1 cut(s) 629
BstDEI CTNAG 3 cut(s) 305, 602, 903
BstF5I GGATG 2 cut(s) 337, 420
BstFNI CGCG 4 cut(s) 243, 417, 447, 778
BstHHI GCGC 3 cut(s) 67, 243, 449
BstKTI GATC 5 cut(s) 12, 256, 311, 696, 898
BstMAI GTCTC 2 cut(s) 166, 517
BstMBI GATC 5 cut(s) 9, 253, 308, 693, 895
BstMWI GCNNNNNNNGC 5 cut(s) 71, 105, 108, 117, 378
BstNI CCWGG 1 cut(s) 962
BstSCI CCNGG 1 cut(s) 960
BstUI CGCG 4 cut(s) 243, 417, 447, 778
BstV1I GCAGC 7 cut(s) 14, 17, 83, 86, 86, 121, 390
BstV2I GAAGAC 1 cut(s) 189
BsuRI GGCC 2 cut(s) 395, 702
BtrI CACGTC 1 cut(s) 220
BtsCI GGATG 2 cut(s) 337, 420
BtsI GCAGTG 3 cut(s) 60, 396, 540
BtsIMutI CAGTG 5 cut(s) 60, 396, 540, 601, 703
Cac8I GCNNGC 1 cut(s) 629
CaiI CAGNNNCTG 2 cut(s) 99, 317
CciI TCATGA 1 cut(s) 12
CfoI GCGC 3 cut(s) 67, 243, 449
Cfr13I GGNCC 2 cut(s) 484, 771
CseI GACGC 2 cut(s) 183, 767
Csp6I GTAC 5 cut(s) 42, 169, 186, 517, 983
CviAII CATG 3 cut(s) 13, 716, 868
CviQI GTAC 5 cut(s) 42, 169, 186, 517, 983
DdeI CTNAG 3 cut(s) 305, 602, 903
DpnI GATC 5 cut(s) 11, 255, 310, 695, 897
DpnII GATC 5 cut(s) 9, 253, 308, 693, 895
DrdI GACNNNNNNGTC 1 cut(s) 271
DriI GACNNNNNGTC 2 cut(s) 410, 777
DseDI GACNNNNNNGTC 1 cut(s) 271
EaeI YGGCCR 1 cut(s) 700
Eam1104I CTCTTC 1 cut(s) 714
Eam1105I GACNNNNNGTC 2 cut(s) 410, 777
EarI CTCTTC 1 cut(s) 714
Eco130I CCWWGG 2 cut(s) 384, 451
Eco31I GGTCTC 1 cut(s) 517
Eco47I GGWCC 2 cut(s) 484, 771
EcoO109I RGGNCCY 1 cut(s) 484
EcoRII CCWGG 1 cut(s) 960
EcoT14I CCWWGG 2 cut(s) 384, 451
ErhI CCWWGG 2 cut(s) 384, 451
Esp3I CGTCTC 1 cut(s) 166
FaeI CATG 3 cut(s) 16, 719, 871
FaiI YATR 8 cut(s) 14, 152, 532, 584, 608, 717, 869, 891
FaqI GGGAC 2 cut(s) 277, 932
FatI CATG 3 cut(s) 12, 715, 867
FbaI TGATCA 2 cut(s) 9, 253
FblI GTMKAC 1 cut(s) 222
Fnu4HI GCNGC 9 cut(s) 28, 31, 75, 97, 100, 109, 135, 379, 844
FokI GGATG 2 cut(s) 344, 407
Fsp4HI GCNGC 9 cut(s) 28, 31, 75, 97, 100, 109, 135, 379, 844
FspBI CTAG 3 cut(s) 45, 548, 644
GlaI GCGC 3 cut(s) 66, 242, 448
GluI GCNGC 9 cut(s) 28, 31, 75, 97, 100, 109, 135, 379, 844
HaeIII GGCC 2 cut(s) 395, 702
HgaI GACGC 2 cut(s) 183, 767
HhaI GCGC 3 cut(s) 67, 243, 449
Hin1II CATG 3 cut(s) 16, 719, 871
Hin6I GCGC 3 cut(s) 65, 241, 447
HinP1I GCGC 3 cut(s) 65, 241, 447
HinfI GANTC 5 cut(s) 209, 287, 658, 688, 709
HphI GGTGA 1 cut(s) 905
Hpy166II GTNNAC 1 cut(s) 223
Hpy188I TCNGA 6 cut(s) 286, 292, 313, 494, 687, 693
Hpy188III TCNNGA 6 cut(s) 13, 434, 509, 572, 769, 881
Hpy8I GTNNAC 1 cut(s) 223
Hpy99I CGWCG 3 cut(s) 362, 783, 926
HpyAV CCTTC 3 cut(s) 113, 431, 567
HpyCH4III ACNGT 1 cut(s) 926
HpyCH4IV ACGT 1 cut(s) 219
HpyCH4V TGCA 6 cut(s) 33, 74, 372, 545, 791, 824
HpyF10VI GCNNNNNNNGC 5 cut(s) 71, 105, 108, 117, 378
HpyF3I CTNAG 3 cut(s) 305, 602, 903
HpySE526I ACGT 1 cut(s) 219
Hsp92II CATG 3 cut(s) 16, 719, 871
HspAI GCGC 3 cut(s) 65, 241, 447
Ksp22I TGATCA 2 cut(s) 9, 253
Kzo9I GATC 5 cut(s) 9, 253, 308, 693, 895
LmnI GCTCC 2 cut(s) 72, 349
LpnPI CCDG 8 cut(s) 106, 242, 607, 754, 853, 894, 947, 974
Lsp1109I GCAGC 7 cut(s) 14, 17, 83, 86, 86, 121, 390
LweI GCATC 1 cut(s) 811
MaeI CTAG 3 cut(s) 45, 548, 644
MaeII ACGT 1 cut(s) 219
MaeIII GTNAC 3 cut(s) 244, 509, 706
MalI GATC 5 cut(s) 11, 255, 310, 695, 897
MboI GATC 5 cut(s) 9, 253, 308, 693, 895
MboII GAAGA 2 cut(s) 189, 731
MfeI CAATTG 1 cut(s) 795
MlsI TGGCCA 1 cut(s) 702
MluCI AATT 5 cut(s) 269, 324, 677, 795, 948
MluNI TGGCCA 1 cut(s) 702
MlyI GAGTC 2 cut(s) 281, 703
MmeI TCCRAC 1 cut(s) 923
MnlI CCTC 9 cut(s) 211, 286, 289, 292, 307, 385, 641, 784, 793
Mox20I TGGCCA 1 cut(s) 702
MscI TGGCCA 1 cut(s) 702
MseI TTAA 1 cut(s) 828
MslI CAYNNNNRTG 1 cut(s) 17
Msp20I TGGCCA 1 cut(s) 702
MspA1I CMGCKG 1 cut(s) 96
MspR9I CCNGG 1 cut(s) 962
MunI CAATTG 1 cut(s) 795
MvaI CCWGG 1 cut(s) 962
MvnI CGCG 4 cut(s) 243, 417, 447, 778
MwoI GCNNNNNNNGC 5 cut(s) 71, 105, 108, 117, 378
NdeII GATC 5 cut(s) 9, 253, 308, 693, 895
NlaIII CATG 3 cut(s) 16, 719, 871
NlaIV GGNNCC 2 cut(s) 457, 486
NmuCI GTSAC 2 cut(s) 509, 706
PagI TCATGA 1 cut(s) 12
PfeI GAWTC 3 cut(s) 209, 658, 688
PflFI GACNNNGTC 1 cut(s) 488
PkrI GCNGC 9 cut(s) 29, 32, 76, 98, 101, 110, 136, 380, 845
PleI GAGTC 2 cut(s) 281, 703
PpsI GAGTC 2 cut(s) 281, 703
PpuMI RGGWCCY 1 cut(s) 484
Psp5II RGGWCCY 1 cut(s) 484
Psp6I CCWGG 1 cut(s) 960
PspGI CCWGG 1 cut(s) 960
PspN4I GGNNCC 2 cut(s) 457, 486
PspPI GGNCC 2 cut(s) 484, 771
PspPPI RGGWCCY 1 cut(s) 484
PstNI CAGNNNCTG 2 cut(s) 99, 317
PsyI GACNNNGTC 1 cut(s) 488
PvuII CAGCTG 1 cut(s) 96
RsaI GTAC 5 cut(s) 43, 170, 187, 518, 984
RsaNI GTAC 5 cut(s) 42, 169, 186, 517, 983
RseI CAYNNNNRTG 1 cut(s) 17
SaqAI TTAA 1 cut(s) 828
SatI GCNGC 9 cut(s) 28, 31, 75, 97, 100, 109, 135, 379, 844
Sau3AI GATC 5 cut(s) 9, 253, 308, 693, 895
Sau96I GGNCC 2 cut(s) 484, 771
ScaI AGTACT 2 cut(s) 170, 187
SchI GAGTC 2 cut(s) 281, 703
ScrFI CCNGG 1 cut(s) 962
SfaNI GCATC 1 cut(s) 811
SinI GGWCC 2 cut(s) 484, 771
SmiMI CAYNNNNRTG 1 cut(s) 17
SmlI CTYRAG 1 cut(s) 425
SmoI CTYRAG 1 cut(s) 425
SpeI ACTAGT 1 cut(s) 44
Sse9I AATT 5 cut(s) 269, 324, 677, 795, 948
SsiI CCGC 3 cut(s) 109, 415, 844
SspMI CTAG 3 cut(s) 45, 548, 644
StyD4I CCNGG 1 cut(s) 960
StyI CCWWGG 2 cut(s) 384, 451
TaaI ACNGT 1 cut(s) 926
TaiI ACGT 1 cut(s) 222
TaqI TCGA 3 cut(s) 403, 590, 712
TasI AATT 5 cut(s) 269, 324, 677, 795, 948
TatI WGTACW 5 cut(s) 41, 168, 185, 516, 982
TauI GCSGC 2 cut(s) 111, 846
TfiI GAWTC 3 cut(s) 209, 658, 688
Tru1I TTAA 1 cut(s) 828
Tru9I TTAA 1 cut(s) 828
TscAI CASTG 5 cut(s) 67, 403, 547, 601, 703
TseFI GTSAC 2 cut(s) 509, 706
TseI GCWGC 7 cut(s) 27, 30, 74, 96, 99, 134, 378
Tsp45I GTSAC 2 cut(s) 509, 706
TspDTI ATGAA 4 cut(s) 35, 732, 735, 739
TspRI CASTG 5 cut(s) 67, 403, 547, 601, 703
Tth111I GACNNNGTC 1 cut(s) 488
VpaK11BI GGWCC 2 cut(s) 484, 771
XmiI GTMKAC 1 cut(s) 222
XspI CTAG 3 cut(s) 45, 548, 644
ZrmI AGTACT 2 cut(s) 170, 187
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.