Rroxscaffold_6G00425940

Uncharacterized conserved protein (DUF2358)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0016218
Physical Location & Seq
Reverse (-)
46873289 .. 46879428
6140 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00425940.1

Sequence Viewer

Length: 1413 bp
ATGGTTCGGTTTCGGTGGAGAACATGGCGAGAGACTTCTTTTAAGTTGGGTGAGGAGACCGAGATTGGGTTGGAGCGGCGTCTCCCTTCTTATAGGGATTGGAAGATTTTTTATGGAGGTACAAAAACGGAATCTTGTCGTGGTGGTAGATATTGTGGTTGCTGTGGGTGCATACGGACGACGGCAATGGTTGTGCTCAAGCACCGAATCGGGCTTCTTATTCTTGGGTTTTCGGATCGGTGGGATGCGGTTCTCACGAGCCCTATGGAAGATTTGGATTTGGGACCCATAGTCGATGGCTTAGCCGATCGGATCCTTCATCTAGGGAGGCCGGTGGCTTTGTTGGGTGCGGTCGAAACTATGGGTGGTCGACAGACGACGACCATCTTTGGGTGCTCGGCGGCGGGTGTCCATATCAAATTTGGCTTTCGAAGGTGCTGCACTACTGGTACTAGTGCTACTACTTTCACTGCGCTCCTTGCAGCAACAACCACCACCACCAGCTGCTGCCTTCGCCGCCGCGAAAGCAAAACTACTACTGCTGCTAATAGTAAATCTATATTTTTCATCTCCAAGTACTCCAAGCCTTTTAGTACTGCGTATTCCTCCCAAGATTCACACCATGTCCACCACCCTTTTTTACTGCGCGTAACAAATGATCAGGGTGGGACTGAATTGTCCTCCTCCTCTGACTCTGATAAAGAATATCTAAGATCAGAGGCTGATAGAATTGTGGATGGCTTGAACTTTGGGGAGCTTTGCGACGAGTTTGAGTGCATTAGCAGCCCCTTGGTGGAGGCCACTGCTCGACAGCTTGTCCGAGACATCCTTGAGCTTCGGGAAGGCAATCGCGCCCTTGGAACCTTTGCTCTTTCGGTTCAATACAAGGACCCCGTCAGAAGTTTTAGTGGTCGTGACAAGTACAAGAGACCACTATGGATAACCACTGCACTAGAGAAGCCTTCTGTGAGTGTTCAAGAAATGAGCATGTTATCGACCAGTGTGCTTAGTATAAAATGGACAATCAAAGGCAAGCCCAAGAACATTCTAGGAGGAGATTTGATTCTAAAAATCAACTCCCAATTCACTCTGAATCAGATCAGTGGCCAAGTGACTCGACATGAAGAGTTTTGGGATTTATCAGCTTCTTCATTCATTTCACAAGCCTATTTCTGGACCTCGCGTCGTCTGTTTGCAACAATTGAGGGTGGAAAAGACTTGACTGATGCAGTTAAAAGTCTTTCCAGCCGCTTCCCAACAGAAAAACAGAACATGGACATTTTTCCAGACCCTTATGGTGATCCAACTAAGTTCTTTCAAAGGGACGACGGTTTCCAAAGAGATGTGTATCAAATTGCTCTGTTCCTGGCACTTGTTTATTTTGTTGTACAACTTTTGAGAACAACCTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2358

Protein Analysis

470

Amino Acids

52.83

Weight (kDa)

8.91

Isoelectric Point (pI)

44.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF2358 PF10184 289 - 374 7.7e-06 Uncharacterized conserved protein (DUF2358)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016218)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G65230
fragaria_vesca FvH4_6g00410
malus_domestica MD04G1245900.v1.1 MD12G1264100.v1.1
prunus_persica Prupe.6G362300_v2.0.a1
pyrus_communis pycom04g21680
rosa_chinensis RchiOBHm_Chr3g0447741
rosa_laevigata RLG00000025974
rosa_multiflora Rmu_ssc0000391.1_g000014
rosa_roxburghii Rroxscaffold_6G00425940
rosa_rugosa Rorug02G0605200 Rorug02G0605200
rosa_samantha Rh3AG004900 Rh3CG004900
rosa_wichuraiana Rw3G000360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 676
AccBSI CCGCTC 1 cut(s) 76
AccI GTMKAC 1 cut(s) 370
AccII CGCG 4 cut(s) 522, 648, 852, 1183
AciI CCGC 8 cut(s) 76, 248, 350, 401, 404, 517, 520, 1249
AclWI GGATC 4 cut(s) 243, 307, 320, 1295
AcoI YGGCCR 1 cut(s) 1105
AcsI RAATTY 1 cut(s) 419
AcyI GRCGYC 1 cut(s) 79
AfaI GTAC 6 cut(s) 121, 451, 578, 595, 923, 1389
AfiI CCNNNNNNNGG 4 cut(s) 66, 390, 793, 1173
AgsI TTSAA 4 cut(s) 745, 881, 977, 1319
AhdI GACNNNNNGTC 3 cut(s) 290, 815, 1182
AhlI ACTAGT 1 cut(s) 452
AjnI CCWGG 1 cut(s) 1365
AluBI AGCT 5 cut(s) 504, 757, 814, 835, 1145
AluI AGCT 5 cut(s) 504, 757, 814, 835, 1145
Alw21I GWGCWC 2 cut(s) 198, 398
Alw26I GTCTC 5 cut(s) 26, 50, 86, 816, 922
AlwI GGATC 4 cut(s) 243, 307, 320, 1295
AlwNI CAGNNNCTG 2 cut(s) 507, 722
AoxI GGCC 3 cut(s) 329, 798, 1105
ApeKI GCWGC 6 cut(s) 438, 482, 504, 507, 542, 783
ApoI RAATTY 1 cut(s) 419
AspLEI GCGC 3 cut(s) 475, 648, 854
AspS9I GGNCC 3 cut(s) 284, 889, 1176
AsuHPI GGTGA 2 cut(s) 62, 1310
AsuII TTCGAA 1 cut(s) 430
AvaII GGWCC 3 cut(s) 284, 889, 1176
BalI TGGCCA 1 cut(s) 1107
BamHI GGATCC 1 cut(s) 312
BanII GRGCYC 1 cut(s) 263
BauI CACGAG 1 cut(s) 256
Bbv12I GWGCWC 2 cut(s) 198, 398
BbvI GCAGC 6 cut(s) 425, 491, 494, 494, 529, 795
BccI CCATC 3 cut(s) 290, 392, 731
BceAI ACGGC 1 cut(s) 198
BcgI CGANNNNNNTGC 4 cut(s) 420, 454, 985, 1019
BciT130I CCWGG 1 cut(s) 1367
BclI TGATCA 1 cut(s) 658
BcoDI GTCTC 5 cut(s) 26, 50, 86, 816, 922
BcuI ACTAGT 1 cut(s) 452
BfaI CTAG 4 cut(s) 323, 453, 953, 1049
BlpI GCTNAGC 1 cut(s) 301
BmcAI AGTACT 2 cut(s) 578, 595
Bme1390I CCNGG 1 cut(s) 1367
Bme18I GGWCC 3 cut(s) 284, 889, 1176
BmeRI GACNNNNNGTC 3 cut(s) 290, 815, 1182
BmgT120I GGNCC 3 cut(s) 284, 889, 1176
BmiI GGNNCC 5 cut(s) 285, 286, 314, 862, 891
BmrFI CCNGG 1 cut(s) 1367
BmsI GCATC 2 cut(s) 235, 1216
Bpu1102I GCTNAGC 1 cut(s) 301
Bpu14I TTCGAA 1 cut(s) 430
BpuEI CTTGAG 2 cut(s) 182, 851
BsaBI GATNNNNATC 1 cut(s) 1347
BsaHI GRCGYC 1 cut(s) 79
BsaI GGTCTC 2 cut(s) 50, 922
BsaJI CCNNGG 2 cut(s) 789, 856
Bsc4I CCNNNNNNNGG 4 cut(s) 66, 390, 793, 1173
Bse118I RCCGGY 1 cut(s) 331
Bse1I ACTGG 2 cut(s) 451, 999
Bse3DI GCAATG 1 cut(s) 192
Bse8I GATNNNNATC 1 cut(s) 1347
BseBI CCWGG 1 cut(s) 1367
BseDI CCNNGG 2 cut(s) 789, 856
BseGI GGATG 3 cut(s) 250, 742, 825
BseJI GATNNNNATC 1 cut(s) 1347
BseLI CCNNNNNNNGG 4 cut(s) 66, 390, 793, 1173
BseMI GCAATG 1 cut(s) 192
BseNI ACTGG 2 cut(s) 451, 999
BseRI GAGGAG 4 cut(s) 68, 673, 676, 1068
BseXI GCAGC 6 cut(s) 425, 491, 494, 494, 529, 795
BsgI GTGCAG 2 cut(s) 424, 933
Bsh1236I CGCG 4 cut(s) 522, 648, 852, 1183
Bsh1285I CGRYCG 2 cut(s) 310, 354
BshFI GGCC 3 cut(s) 331, 800, 1107
BsiEI CGRYCG 2 cut(s) 310, 354
BsiHKAI GWGCWC 2 cut(s) 198, 398
BsiSI CCGG 1 cut(s) 332
BslFI GGGAC 3 cut(s) 297, 682, 1337
BslI CCNNNNNNNGG 4 cut(s) 66, 390, 793, 1173
BsmAI GTCTC 5 cut(s) 26, 50, 86, 816, 922
BsmBI CGTCTC 1 cut(s) 86
BsmFI GGGAC 3 cut(s) 297, 682, 1337
BsnI GGCC 3 cut(s) 331, 800, 1107
Bso31I GGTCTC 2 cut(s) 50, 922
Bsp119I TTCGAA 1 cut(s) 430
Bsp1286I GDGCHC 3 cut(s) 198, 263, 398
Bsp1407I TGTACA 1 cut(s) 1387
Bsp143I GATC 7 cut(s) 235, 307, 312, 658, 713, 1098, 1300
Bsp1720I GCTNAGC 1 cut(s) 301
BspACI CCGC 8 cut(s) 76, 248, 350, 401, 404, 517, 520, 1249
BspANI GGCC 3 cut(s) 331, 800, 1107
BspFNI CGCG 4 cut(s) 522, 648, 852, 1183
BspLI GGNNCC 5 cut(s) 285, 286, 314, 862, 891
BspPI GGATC 4 cut(s) 243, 307, 320, 1295
BspT104I TTCGAA 1 cut(s) 430
BspTNI GGTCTC 2 cut(s) 50, 922
BsrBI CCGCTC 1 cut(s) 76
BsrDI GCAATG 1 cut(s) 192
BsrFI RCCGGY 1 cut(s) 331
BsrGI TGTACA 1 cut(s) 1387
BsrI ACTGG 2 cut(s) 451, 999
BssAI RCCGGY 1 cut(s) 331
BssECI CCNNGG 2 cut(s) 789, 856
BssMI GATC 7 cut(s) 235, 307, 312, 658, 713, 1098, 1300
BssNI GRCGYC 1 cut(s) 79
BssSI CACGAG 1 cut(s) 256
BssT1I CCWWGG 2 cut(s) 789, 856
Bst2BI CACGAG 1 cut(s) 256
Bst2UI CCWGG 1 cut(s) 1367
Bst4CI ACNGT 1 cut(s) 1331
Bst6I CTCTTC 1 cut(s) 1119
BstACI GRCGYC 1 cut(s) 79
BstAUI TGTACA 1 cut(s) 1387
BstBI TTCGAA 1 cut(s) 430
BstC8I GCNNGC 1 cut(s) 1034
BstDEI CTNAG 4 cut(s) 301, 710, 1007, 1308
BstF5I GGATG 3 cut(s) 250, 742, 825
BstFNI CGCG 4 cut(s) 522, 648, 852, 1183
BstHHI GCGC 3 cut(s) 475, 648, 854
BstKTI GATC 7 cut(s) 238, 310, 315, 661, 716, 1101, 1303
BstMAI GTCTC 5 cut(s) 26, 50, 86, 816, 922
BstMBI GATC 7 cut(s) 235, 307, 312, 658, 713, 1098, 1300
BstMCI CGRYCG 2 cut(s) 310, 354
BstMWI GCNNNNNNNGC 6 cut(s) 168, 479, 513, 516, 525, 783
BstNI CCWGG 1 cut(s) 1367
BstNSI RCATGY 1 cut(s) 991
BstSCI CCNGG 1 cut(s) 1365
BstUI CGCG 4 cut(s) 522, 648, 852, 1183
BstV1I GCAGC 6 cut(s) 425, 491, 494, 494, 529, 795
BstX2I RGATCY 1 cut(s) 312
BstYI RGATCY 1 cut(s) 312
BsuRI GGCC 3 cut(s) 331, 800, 1107
BtsCI GGATG 3 cut(s) 250, 742, 825
BtsI GCAGTG 3 cut(s) 468, 801, 945
BtsIMutI CAGTG 5 cut(s) 468, 801, 945, 1006, 1108
Cac8I GCNNGC 1 cut(s) 1034
CaiI CAGNNNCTG 2 cut(s) 507, 722
CfoI GCGC 3 cut(s) 475, 648, 854
Cfr10I RCCGGY 1 cut(s) 331
Cfr13I GGNCC 3 cut(s) 284, 889, 1176
CseI GACGC 2 cut(s) 68, 1172
Csp6I GTAC 6 cut(s) 120, 450, 577, 594, 922, 1388
CviAII CATG 5 cut(s) 24, 623, 988, 1121, 1273
CviQI GTAC 6 cut(s) 120, 450, 577, 594, 922, 1388
DdeI CTNAG 4 cut(s) 301, 710, 1007, 1308
DpnI GATC 7 cut(s) 237, 309, 314, 660, 715, 1100, 1302
DpnII GATC 7 cut(s) 235, 307, 312, 658, 713, 1098, 1300
DrdI GACNNNNNNGTC 1 cut(s) 676
DriI GACNNNNNGTC 3 cut(s) 290, 815, 1182
DseDI GACNNNNNNGTC 1 cut(s) 676
EaeI YGGCCR 1 cut(s) 1105
Eam1104I CTCTTC 1 cut(s) 1119
Eam1105I GACNNNNNGTC 3 cut(s) 290, 815, 1182
EarI CTCTTC 1 cut(s) 1119
Eco130I CCWWGG 2 cut(s) 789, 856
Eco24I GRGCYC 1 cut(s) 263
Eco31I GGTCTC 2 cut(s) 50, 922
Eco47I GGWCC 3 cut(s) 284, 889, 1176
EcoO109I RGGNCCY 2 cut(s) 284, 889
EcoRII CCWGG 1 cut(s) 1365
EcoT14I CCWWGG 2 cut(s) 789, 856
EcoT38I GRGCYC 1 cut(s) 263
ErhI CCWWGG 2 cut(s) 789, 856
Esp3I CGTCTC 1 cut(s) 86
FaeI CATG 5 cut(s) 27, 626, 991, 1124, 1276
FaqI GGGAC 3 cut(s) 297, 682, 1337
FatI CATG 5 cut(s) 23, 622, 987, 1120, 1272
FauI CCCGC 1 cut(s) 397
FbaI TGATCA 1 cut(s) 658
FblI GTMKAC 1 cut(s) 370
FokI GGATG 3 cut(s) 257, 749, 812
FriOI GRGCYC 1 cut(s) 263
FspBI CTAG 4 cut(s) 323, 453, 953, 1049
GlaI GCGC 3 cut(s) 474, 647, 853
HaeIII GGCC 3 cut(s) 331, 800, 1107
HapII CCGG 1 cut(s) 332
HgaI GACGC 2 cut(s) 68, 1172
HhaI GCGC 3 cut(s) 475, 648, 854
Hin1I GRCGYC 1 cut(s) 79
Hin1II CATG 5 cut(s) 27, 626, 991, 1124, 1276
Hin6I GCGC 3 cut(s) 473, 646, 852
HinP1I GCGC 3 cut(s) 473, 646, 852
HincII GTYRAC 1 cut(s) 371
HindII GTYRAC 1 cut(s) 371
HinfI GANTC 7 cut(s) 131, 207, 614, 692, 1063, 1093, 1114
HpaII CCGG 1 cut(s) 332
HphI GGTGA 2 cut(s) 62, 1310
Hpy166II GTNNAC 2 cut(s) 371, 628
Hpy188I TCNGA 9 cut(s) 235, 312, 691, 697, 718, 821, 899, 1092, 1098
Hpy188III TCNNGA 6 cut(s) 256, 839, 914, 977, 1174, 1286
Hpy8I GTNNAC 2 cut(s) 371, 628
Hpy99I CGWCG 5 cut(s) 184, 382, 767, 1188, 1331
HpyAV CCTTC 6 cut(s) 96, 326, 426, 521, 836, 972
HpyCH4III ACNGT 1 cut(s) 1331
HpyCH4V TGCA 7 cut(s) 171, 441, 482, 777, 950, 1196, 1229
HpyF10VI GCNNNNNNNGC 6 cut(s) 168, 479, 513, 516, 525, 783
HpyF3I CTNAG 4 cut(s) 301, 710, 1007, 1308
Hsp92I GRCGYC 1 cut(s) 79
Hsp92II CATG 5 cut(s) 27, 626, 991, 1124, 1276
HspAI GCGC 3 cut(s) 473, 646, 852
KflI GGGWCCC 1 cut(s) 284
Ksp22I TGATCA 1 cut(s) 658
Kzo9I GATC 7 cut(s) 235, 307, 312, 658, 713, 1098, 1300
LmnI GCTCC 3 cut(s) 73, 480, 754
Lsp1109I GCAGC 6 cut(s) 425, 491, 494, 494, 529, 795
LweI GCATC 2 cut(s) 235, 1216
MaeI CTAG 4 cut(s) 323, 453, 953, 1049
MaeIII GTNAC 3 cut(s) 649, 914, 1111
MalI GATC 7 cut(s) 237, 309, 314, 660, 715, 1100, 1302
MbiI CCGCTC 1 cut(s) 76
MboI GATC 7 cut(s) 235, 307, 312, 658, 713, 1098, 1300
MboII GAAGA 4 cut(s) 115, 281, 1136, 1140
MfeI CAATTG 1 cut(s) 1200
MflI RGATCY 1 cut(s) 312
MhlI GDGCHC 3 cut(s) 198, 263, 398
MlsI TGGCCA 1 cut(s) 1107
MluCI AATT 6 cut(s) 419, 674, 729, 1082, 1200, 1353
MluNI TGGCCA 1 cut(s) 1107
MlyI GAGTC 2 cut(s) 686, 1108
MmeI TCCRAC 2 cut(s) 51, 1328
Mox20I TGGCCA 1 cut(s) 1107
MscI TGGCCA 1 cut(s) 1107
MseI TTAA 2 cut(s) 42, 1233
Msp20I TGGCCA 1 cut(s) 1107
MspA1I CMGCKG 1 cut(s) 504
MspI CCGG 1 cut(s) 332
MspR9I CCNGG 1 cut(s) 1367
MunI CAATTG 1 cut(s) 1200
MvaI CCWGG 1 cut(s) 1367
MvnI CGCG 4 cut(s) 522, 648, 852, 1183
MwoI GCNNNNNNNGC 6 cut(s) 168, 479, 513, 516, 525, 783
NdeII GATC 7 cut(s) 235, 307, 312, 658, 713, 1098, 1300
NlaIII CATG 5 cut(s) 27, 626, 991, 1124, 1276
NlaIV GGNNCC 5 cut(s) 285, 286, 314, 862, 891
NmeAIII GCCGAG 1 cut(s) 377
NmuCI GTSAC 2 cut(s) 914, 1111
NspI RCATGY 1 cut(s) 991
NspV TTCGAA 1 cut(s) 430
PfeI GAWTC 5 cut(s) 131, 207, 614, 1063, 1093
PflFI GACNNNGTC 1 cut(s) 893
Ple19I CGATCG 1 cut(s) 310
PleI GAGTC 2 cut(s) 686, 1108
PpsI GAGTC 2 cut(s) 686, 1108
PpuMI RGGWCCY 2 cut(s) 284, 889
Psp5II RGGWCCY 2 cut(s) 284, 889
Psp6I CCWGG 1 cut(s) 1365
PspGI CCWGG 1 cut(s) 1365
PspN4I GGNNCC 5 cut(s) 285, 286, 314, 862, 891
PspPI GGNCC 3 cut(s) 284, 889, 1176
PspPPI RGGWCCY 2 cut(s) 284, 889
PstNI CAGNNNCTG 2 cut(s) 507, 722
PsuI RGATCY 1 cut(s) 312
PsyI GACNNNGTC 1 cut(s) 893
PvuI CGATCG 1 cut(s) 310
PvuII CAGCTG 1 cut(s) 504
RsaI GTAC 6 cut(s) 121, 451, 578, 595, 923, 1389
RsaNI GTAC 6 cut(s) 120, 450, 577, 594, 922, 1388
SalI GTCGAC 1 cut(s) 369
SaqAI TTAA 2 cut(s) 42, 1233
Sau3AI GATC 7 cut(s) 235, 307, 312, 658, 713, 1098, 1300
Sau96I GGNCC 3 cut(s) 284, 889, 1176
ScaI AGTACT 2 cut(s) 578, 595
SchI GAGTC 2 cut(s) 686, 1108
ScrFI CCNGG 1 cut(s) 1367
SduI GDGCHC 3 cut(s) 198, 263, 398
SfaNI GCATC 2 cut(s) 235, 1216
SfuI TTCGAA 1 cut(s) 430
SinI GGWCC 3 cut(s) 284, 889, 1176
SmlI CTYRAG 2 cut(s) 197, 830
SmoI CTYRAG 2 cut(s) 197, 830
SpeI ACTAGT 1 cut(s) 452
Sse9I AATT 6 cut(s) 419, 674, 729, 1082, 1200, 1353
SsiI CCGC 8 cut(s) 76, 248, 350, 401, 404, 517, 520, 1249
SspMI CTAG 4 cut(s) 323, 453, 953, 1049
StyD4I CCNGG 1 cut(s) 1365
StyI CCWWGG 2 cut(s) 789, 856
TaaI ACNGT 1 cut(s) 1331
TaqI TCGA 7 cut(s) 294, 354, 370, 430, 808, 995, 1117
TaqII GACCGA 1 cut(s) 74
TasI AATT 6 cut(s) 419, 674, 729, 1082, 1200, 1353
TatI WGTACW 4 cut(s) 576, 593, 921, 1387
TauI GCSGC 5 cut(s) 79, 404, 519, 522, 1251
TfiI GAWTC 5 cut(s) 131, 207, 614, 1063, 1093
Tru1I TTAA 2 cut(s) 42, 1233
Tru9I TTAA 2 cut(s) 42, 1233
TscAI CASTG 5 cut(s) 475, 808, 952, 1006, 1108
TseFI GTSAC 2 cut(s) 914, 1111
TseI GCWGC 6 cut(s) 438, 482, 504, 507, 542, 783
Tsp45I GTSAC 2 cut(s) 914, 1111
TspDTI ATGAA 5 cut(s) 308, 556, 1137, 1140, 1144
TspGWI ACGGA 2 cut(s) 143, 190
TspRI CASTG 5 cut(s) 475, 808, 952, 1006, 1108
Tth111I GACNNNGTC 1 cut(s) 893
VpaK11BI GGWCC 3 cut(s) 284, 889, 1176
XapI RAATTY 1 cut(s) 419
XceI RCATGY 1 cut(s) 991
XcmI CCANNNNNNNNNTGG 1 cut(s) 419
XmiI GTMKAC 1 cut(s) 370
XspI CTAG 4 cut(s) 323, 453, 953, 1049
ZrmI AGTACT 2 cut(s) 578, 595
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.