Rh3AG004900

Uncharacterized conserved protein (DUF2358)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3A
OG0016218
Physical Location & Seq
Reverse (-)
363414 .. 364953
1540 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3AG004900.1

Sequence Viewer

Length: 1005 bp
ATGATGATGATCATGATGATGAATAGCTGCTGCACTACTGGTACTAGTGCTACTACTTTCACTGCGCTCCTTGCAGCAACAACCACCACCACCAGCTGCTGCCTTCGCCGCCGCGAAAGCAAAACTACTACTGCTGCTAATAGTAAATCTATATTTTTCATCTCCAAGTACTCCAAGCCTTTTAGTACTGCGTCTTCCTCCCAAGATTCACACCATGTCCACCACCCTTTTTTACTGCGCGTAACAAATGATCAGGGTAGGACTGAATTGTCCTCCTCCTCTGACTCTGATAAAGAATATCTAAGATCAGAGGCTGATAGAATTGTGGATGGCTTGAACTTTGGGGAGCTTTGCGACGAGTTTGAGTGCATTAGCAGCCCCTTGGTGGAGGCCACTGCTCGACAGCTTGTCCGAGACATCCTTGAGCTTCGGGAAGGCAATCGCGCCCTTGGAACCTTTGCTCTTTCCGTTCAATACAAGGACCCCGTCAGAAGTTTTAGTGGTCGTGACAAGTACAAGAGACCACTATGGATAACCACTGCACTAGAGAAGCCTTCTGTGAGTGTTCAAGAAATGAGCATGTTATCGACCAGTGTGCTTAGTATAAAATGGACAATCAAAGGCAAGCCCAAGAACATTCTAGGAGGAGATTTGATTCTAAAAATCAACTCCCAATTCACTCTGAATCAGATCAGTGGCCAAGTGACTCGACATGAAGAGTTTTGGGATTTATCAGCTTCTTCATTCATTTCACAAGCCTATTTCTGGACCTCGCGTCGTCTGTTTGCAACAATTGAGGGTGGAAAAGACTTGACTGATGCAGTTAAAAGTCTTTCCAGCCGCTTCCCAACAGAAAAACAGAACATGGACATTTTTCCAGACCCTTATGGTGATCCAACTAAGTTCTTTCAAAGGGACGACGGTTTCCAAAGAGATGTGTATCAAATTGCTCTGTTCCTGGCACTTGTTTATTTTGTTGTACAACTTTTGAGAACAACCTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2358

Protein Analysis

334

Amino Acids

37.66

Weight (kDa)

8.45

Isoelectric Point (pI)

45.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF2358 PF10184 153 - 238 4.2e-06 Uncharacterized conserved protein (DUF2358)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016218)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G65230
fragaria_vesca FvH4_6g00410
malus_domestica MD04G1245900.v1.1 MD12G1264100.v1.1
prunus_persica Prupe.6G362300_v2.0.a1
pyrus_communis pycom04g21680
rosa_chinensis RchiOBHm_Chr3g0447741
rosa_laevigata RLG00000025974
rosa_multiflora Rmu_ssc0000391.1_g000014
rosa_roxburghii Rroxscaffold_6G00425940
rosa_rugosa Rorug02G0605200 Rorug02G0605200
rosa_samantha Rh3AG004900 Rh3CG004900
rosa_wichuraiana Rw3G000360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 268
AccII CGCG 4 cut(s) 114, 240, 444, 775
AciI CCGC 3 cut(s) 109, 112, 841
AclWI GGATC 1 cut(s) 887
AcoI YGGCCR 1 cut(s) 697
AfaI GTAC 5 cut(s) 43, 170, 187, 515, 981
AfiI CCNNNNNNNGG 2 cut(s) 385, 765
AgsI TTSAA 4 cut(s) 337, 473, 569, 911
AhdI GACNNNNNGTC 2 cut(s) 407, 774
AhlI ACTAGT 1 cut(s) 44
AjnI CCWGG 1 cut(s) 957
AluBI AGCT 6 cut(s) 27, 96, 349, 406, 427, 737
AluI AGCT 6 cut(s) 27, 96, 349, 406, 427, 737
Alw26I GTCTC 2 cut(s) 408, 514
AlwI GGATC 1 cut(s) 887
AlwNI CAGNNNCTG 2 cut(s) 99, 314
AoxI GGCC 2 cut(s) 390, 697
ApeKI GCWGC 7 cut(s) 27, 30, 74, 96, 99, 134, 375
AspLEI GCGC 3 cut(s) 67, 240, 446
AspS9I GGNCC 2 cut(s) 481, 768
AsuHPI GGTGA 1 cut(s) 902
AvaII GGWCC 2 cut(s) 481, 768
BalI TGGCCA 1 cut(s) 699
BarI GAAGNNNNNNTAC 2 cut(s) 178, 210
BbsI GAAGAC 1 cut(s) 186
BbvI GCAGC 7 cut(s) 14, 17, 83, 86, 86, 121, 387
BccI CCATC 1 cut(s) 323
BcgI CGANNNNNNTGC 2 cut(s) 577, 611
BciT130I CCWGG 1 cut(s) 959
BclI TGATCA 2 cut(s) 9, 250
BcoDI GTCTC 2 cut(s) 408, 514
BcuI ACTAGT 1 cut(s) 44
BfaI CTAG 3 cut(s) 45, 545, 641
BmcAI AGTACT 2 cut(s) 170, 187
Bme1390I CCNGG 1 cut(s) 959
Bme18I GGWCC 2 cut(s) 481, 768
BmeRI GACNNNNNGTC 2 cut(s) 407, 774
BmgT120I GGNCC 2 cut(s) 481, 768
BmiI GGNNCC 2 cut(s) 454, 483
BmrFI CCNGG 1 cut(s) 959
BmsI GCATC 1 cut(s) 808
BpiI GAAGAC 1 cut(s) 186
BpuEI CTTGAG 1 cut(s) 443
BsaBI GATNNNNATC 2 cut(s) 8, 939
BsaI GGTCTC 1 cut(s) 514
BsaJI CCNNGG 2 cut(s) 381, 448
Bsc4I CCNNNNNNNGG 2 cut(s) 385, 765
Bse1I ACTGG 2 cut(s) 43, 591
Bse8I GATNNNNATC 2 cut(s) 8, 939
BseBI CCWGG 1 cut(s) 959
BseDI CCNNGG 2 cut(s) 381, 448
BseGI GGATG 2 cut(s) 334, 417
BseJI GATNNNNATC 2 cut(s) 8, 939
BseLI CCNNNNNNNGG 2 cut(s) 385, 765
BseNI ACTGG 2 cut(s) 43, 591
BseRI GAGGAG 3 cut(s) 265, 268, 660
BseXI GCAGC 7 cut(s) 14, 17, 83, 86, 86, 121, 387
BsgI GTGCAG 2 cut(s) 16, 525
Bsh1236I CGCG 4 cut(s) 114, 240, 444, 775
BshFI GGCC 2 cut(s) 392, 699
BslFI GGGAC 1 cut(s) 929
BslI CCNNNNNNNGG 2 cut(s) 385, 765
BsmAI GTCTC 2 cut(s) 408, 514
BsmFI GGGAC 1 cut(s) 929
BsnI GGCC 2 cut(s) 392, 699
Bso31I GGTCTC 1 cut(s) 514
Bsp1407I TGTACA 1 cut(s) 979
Bsp143I GATC 5 cut(s) 9, 250, 305, 690, 892
BspACI CCGC 3 cut(s) 109, 112, 841
BspANI GGCC 2 cut(s) 392, 699
BspFNI CGCG 4 cut(s) 114, 240, 444, 775
BspHI TCATGA 1 cut(s) 12
BspLI GGNNCC 2 cut(s) 454, 483
BspPI GGATC 1 cut(s) 887
BspTNI GGTCTC 1 cut(s) 514
BsrGI TGTACA 1 cut(s) 979
BsrI ACTGG 2 cut(s) 43, 591
BssECI CCNNGG 2 cut(s) 381, 448
BssMI GATC 5 cut(s) 9, 250, 305, 690, 892
BssT1I CCWWGG 2 cut(s) 381, 448
Bst2UI CCWGG 1 cut(s) 959
Bst4CI ACNGT 1 cut(s) 923
Bst6I CTCTTC 1 cut(s) 711
BstAUI TGTACA 1 cut(s) 979
BstC8I GCNNGC 1 cut(s) 626
BstDEI CTNAG 3 cut(s) 302, 599, 900
BstF5I GGATG 2 cut(s) 334, 417
BstFNI CGCG 4 cut(s) 114, 240, 444, 775
BstHHI GCGC 3 cut(s) 67, 240, 446
BstKTI GATC 5 cut(s) 12, 253, 308, 693, 895
BstMAI GTCTC 2 cut(s) 408, 514
BstMBI GATC 5 cut(s) 9, 250, 305, 690, 892
BstMWI GCNNNNNNNGC 5 cut(s) 71, 105, 108, 117, 375
BstNI CCWGG 1 cut(s) 959
BstNSI RCATGY 1 cut(s) 583
BstSCI CCNGG 1 cut(s) 957
BstUI CGCG 4 cut(s) 114, 240, 444, 775
BstV1I GCAGC 7 cut(s) 14, 17, 83, 86, 86, 121, 387
BstV2I GAAGAC 1 cut(s) 186
BsuRI GGCC 2 cut(s) 392, 699
BtsCI GGATG 2 cut(s) 334, 417
BtsI GCAGTG 3 cut(s) 60, 393, 537
BtsIMutI CAGTG 5 cut(s) 60, 393, 537, 598, 700
Cac8I GCNNGC 1 cut(s) 626
CaiI CAGNNNCTG 2 cut(s) 99, 314
CciI TCATGA 1 cut(s) 12
CfoI GCGC 3 cut(s) 67, 240, 446
Cfr13I GGNCC 2 cut(s) 481, 768
CseI GACGC 2 cut(s) 180, 764
Csp6I GTAC 5 cut(s) 42, 169, 186, 514, 980
CviAII CATG 5 cut(s) 13, 215, 580, 713, 865
CviQI GTAC 5 cut(s) 42, 169, 186, 514, 980
DdeI CTNAG 3 cut(s) 302, 599, 900
DpnI GATC 5 cut(s) 11, 252, 307, 692, 894
DpnII GATC 5 cut(s) 9, 250, 305, 690, 892
DrdI GACNNNNNNGTC 1 cut(s) 268
DriI GACNNNNNGTC 2 cut(s) 407, 774
DseDI GACNNNNNNGTC 1 cut(s) 268
EaeI YGGCCR 1 cut(s) 697
Eam1104I CTCTTC 1 cut(s) 711
Eam1105I GACNNNNNGTC 2 cut(s) 407, 774
EarI CTCTTC 1 cut(s) 711
Eco130I CCWWGG 2 cut(s) 381, 448
Eco31I GGTCTC 1 cut(s) 514
Eco47I GGWCC 2 cut(s) 481, 768
EcoO109I RGGNCCY 1 cut(s) 481
EcoRII CCWGG 1 cut(s) 957
EcoT14I CCWWGG 2 cut(s) 381, 448
ErhI CCWWGG 2 cut(s) 381, 448
FaeI CATG 5 cut(s) 16, 218, 583, 716, 868
FaiI YATR 9 cut(s) 14, 152, 216, 529, 581, 605, 714, 866, 888
FaqI GGGAC 1 cut(s) 929
FatI CATG 5 cut(s) 12, 214, 579, 712, 864
FbaI TGATCA 2 cut(s) 9, 250
FokI GGATG 2 cut(s) 341, 404
FspBI CTAG 3 cut(s) 45, 545, 641
GlaI GCGC 3 cut(s) 66, 239, 445
HaeIII GGCC 2 cut(s) 392, 699
HgaI GACGC 2 cut(s) 180, 764
HhaI GCGC 3 cut(s) 67, 240, 446
Hin1II CATG 5 cut(s) 16, 218, 583, 716, 868
Hin6I GCGC 3 cut(s) 65, 238, 444
HinP1I GCGC 3 cut(s) 65, 238, 444
HinfI GANTC 5 cut(s) 206, 284, 655, 685, 706
HphI GGTGA 1 cut(s) 902
Hpy166II GTNNAC 1 cut(s) 220
Hpy188I TCNGA 7 cut(s) 283, 289, 310, 413, 491, 684, 690
Hpy188III TCNNGA 6 cut(s) 13, 431, 506, 569, 766, 878
Hpy8I GTNNAC 1 cut(s) 220
Hpy99I CGWCG 3 cut(s) 359, 780, 923
HpyAV CCTTC 3 cut(s) 113, 428, 564
HpyCH4III ACNGT 1 cut(s) 923
HpyCH4V TGCA 6 cut(s) 33, 74, 369, 542, 788, 821
HpyF10VI GCNNNNNNNGC 5 cut(s) 71, 105, 108, 117, 375
HpyF3I CTNAG 3 cut(s) 302, 599, 900
Hsp92II CATG 5 cut(s) 16, 218, 583, 716, 868
HspAI GCGC 3 cut(s) 65, 238, 444
Ksp22I TGATCA 2 cut(s) 9, 250
Kzo9I GATC 5 cut(s) 9, 250, 305, 690, 892
LmnI GCTCC 2 cut(s) 72, 346
LpnPI CCDG 9 cut(s) 24, 106, 239, 604, 751, 850, 891, 944, 971
Lsp1109I GCAGC 7 cut(s) 14, 17, 83, 86, 86, 121, 387
LweI GCATC 1 cut(s) 808
MaeI CTAG 3 cut(s) 45, 545, 641
MaeIII GTNAC 3 cut(s) 241, 506, 703
MalI GATC 5 cut(s) 11, 252, 307, 692, 894
MboI GATC 5 cut(s) 9, 250, 305, 690, 892
MboII GAAGA 3 cut(s) 186, 728, 732
MfeI CAATTG 1 cut(s) 792
MlsI TGGCCA 1 cut(s) 699
MluCI AATT 5 cut(s) 266, 321, 674, 792, 945
MluNI TGGCCA 1 cut(s) 699
MlyI GAGTC 2 cut(s) 278, 700
MmeI TCCRAC 1 cut(s) 920
MnlI CCTC 9 cut(s) 208, 283, 286, 289, 304, 382, 638, 781, 790
Mox20I TGGCCA 1 cut(s) 699
MscI TGGCCA 1 cut(s) 699
MseI TTAA 1 cut(s) 825
MslI CAYNNNNRTG 1 cut(s) 17
Msp20I TGGCCA 1 cut(s) 699
MspA1I CMGCKG 1 cut(s) 96
MspR9I CCNGG 1 cut(s) 959
MunI CAATTG 1 cut(s) 792
MvaI CCWGG 1 cut(s) 959
MvnI CGCG 4 cut(s) 114, 240, 444, 775
MwoI GCNNNNNNNGC 5 cut(s) 71, 105, 108, 117, 375
NdeII GATC 5 cut(s) 9, 250, 305, 690, 892
NlaIII CATG 5 cut(s) 16, 218, 583, 716, 868
NlaIV GGNNCC 2 cut(s) 454, 483
NmuCI GTSAC 2 cut(s) 506, 703
NspI RCATGY 1 cut(s) 583
PagI TCATGA 1 cut(s) 12
PfeI GAWTC 3 cut(s) 206, 655, 685
PflFI GACNNNGTC 1 cut(s) 485
PleI GAGTC 2 cut(s) 278, 700
PpsI GAGTC 2 cut(s) 278, 700
PpuMI RGGWCCY 1 cut(s) 481
Psp5II RGGWCCY 1 cut(s) 481
Psp6I CCWGG 1 cut(s) 957
PspGI CCWGG 1 cut(s) 957
PspN4I GGNNCC 2 cut(s) 454, 483
PspPI GGNCC 2 cut(s) 481, 768
PspPPI RGGWCCY 1 cut(s) 481
PstNI CAGNNNCTG 2 cut(s) 99, 314
PsyI GACNNNGTC 1 cut(s) 485
PvuII CAGCTG 1 cut(s) 96
RsaI GTAC 5 cut(s) 43, 170, 187, 515, 981
RsaNI GTAC 5 cut(s) 42, 169, 186, 514, 980
RseI CAYNNNNRTG 1 cut(s) 17
SaqAI TTAA 1 cut(s) 825
Sau3AI GATC 5 cut(s) 9, 250, 305, 690, 892
Sau96I GGNCC 2 cut(s) 481, 768
ScaI AGTACT 2 cut(s) 170, 187
SchI GAGTC 2 cut(s) 278, 700
ScrFI CCNGG 1 cut(s) 959
SetI ASST 9 cut(s) 29, 98, 351, 408, 429, 458, 739, 773, 1001
SfaNI GCATC 1 cut(s) 808
SinI GGWCC 2 cut(s) 481, 768
SmiMI CAYNNNNRTG 1 cut(s) 17
SmlI CTYRAG 1 cut(s) 422
SmoI CTYRAG 1 cut(s) 422
SpeI ACTAGT 1 cut(s) 44
Sse9I AATT 5 cut(s) 266, 321, 674, 792, 945
SsiI CCGC 3 cut(s) 109, 112, 841
SspMI CTAG 3 cut(s) 45, 545, 641
StyD4I CCNGG 1 cut(s) 957
StyI CCWWGG 2 cut(s) 381, 448
TaaI ACNGT 1 cut(s) 923
TaqI TCGA 3 cut(s) 400, 587, 709
TasI AATT 5 cut(s) 266, 321, 674, 792, 945
TatI WGTACW 4 cut(s) 168, 185, 513, 979
TauI GCSGC 3 cut(s) 111, 114, 843
TfiI GAWTC 3 cut(s) 206, 655, 685
Tru1I TTAA 1 cut(s) 825
Tru9I TTAA 1 cut(s) 825
TscAI CASTG 5 cut(s) 67, 400, 544, 598, 700
TseFI GTSAC 2 cut(s) 506, 703
TseI GCWGC 7 cut(s) 27, 30, 74, 96, 99, 134, 375
Tsp45I GTSAC 2 cut(s) 506, 703
TspDTI ATGAA 5 cut(s) 35, 148, 729, 732, 736
TspGWI ACGGA 1 cut(s) 457
TspRI CASTG 5 cut(s) 67, 400, 544, 598, 700
Tth111I GACNNNGTC 1 cut(s) 485
VpaK11BI GGWCC 2 cut(s) 481, 768
XceI RCATGY 1 cut(s) 583
XspI CTAG 3 cut(s) 45, 545, 641
ZrmI AGTACT 2 cut(s) 170, 187
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.