MD12G1264100.v1.1

Uncharacterized conserved protein (DUF2358)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0016218
Physical Location & Seq
Reverse (-)
33038679 .. 33040857
2179 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1264100.v1.1.491

Sequence Viewer

Length: 987 bp
ATGAGTGCCTCCGTTTCCATCACTGCCCTCGTTGCCTCTAACCCCGCTGCTGCTGCCGCCACCGGCACCCTCCACCGCAAAACTACTAGTTCCTCCACTATCACCACCACTACTAGTACTGCAGCTAAACGAACAATTTTCACCTCCACCTCCAATTCCAAGCCTAATTTTACTGCCTCCTCTTCCTCCTCCTCCCACTACCACCACCCCTTCATACTGCGAGTAACAAATGATCCGACTAGTAGTAGGAGCACTACTGAATCAGAATTGTCGACGACGGATAAAAGAACGGAGGCCGATAGGATTGTTGATGGATTGAACTTTGGGGAGCTATGCAATGAGTTTGAGTGCATTAGTAGCCCCTTGGTGGAGTCTACAGCTCGACAACTTGTACGCGATATCCTTGAGCTACGAGAAGGCAATCGTGCCCTTGGAACCTTTGCTGTTTCTGTTAAATACAAGGACCCAGTTAGAAGTTTTAGCGGACGTGACAAGTACAAGAGACCGCTATGGATAACCAGTGCACTAGAAAAGCCCTCTGTGAGTGTGCAAGAAATGAGCATGTTATCGACGAGTGTCCTGAGTATCAAGTGGACAATCAAAGGGAAGGCGAAGAACATTATAGGAGGAGATTTGATCATAAGAATAAATTCCCAGTTCACTCTAAACCAGATAAGTGGCCAAGTCATTGAACATGAAGAGTTTTGGGATTTATCAGCTTCATCAGCCATTGCGAAAGCATATTTCTGGACATCACGCCGTCTTTATGCTACAATTGAGGGTGGAAAAGACTTGGTTGATCTTGCTAAAACCATTTCCAGCAAGTTCCCTAGAGAAAAACAGAACATGGAAATTTATCCAGACCCTTACGGTGATCCAACGAAGTTCTTTCAAAGGGATGATGGTTTCCAGAGAGATGTATACCAAATTGGACTGTTTCTGGCACTGGTTTATTTTGTTGTACAGCTTTTGAAGACAACCTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2358

Protein Analysis

329

Amino Acids

36.34

Weight (kDa)

9.18

Isoelectric Point (pI)

41.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF2358 PF10184 147 - 232 2.2e-06 Uncharacterized conserved protein (DUF2358)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016218)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G65230
fragaria_vesca FvH4_6g00410
malus_domestica MD04G1245900.v1.1 MD12G1264100.v1.1
prunus_persica Prupe.6G362300_v2.0.a1
pyrus_communis pycom04g21680
rosa_chinensis RchiOBHm_Chr3g0447741
rosa_laevigata RLG00000025974
rosa_multiflora Rmu_ssc0000391.1_g000014
rosa_roxburghii Rroxscaffold_6G00425940
rosa_rugosa Rorug02G0605200 Rorug02G0605200
rosa_samantha Rh3AG004900 Rh3CG004900
rosa_wichuraiana Rw3G000360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 65
AccI GTMKAC 3 cut(s) 272, 374, 921
AccII CGCG 1 cut(s) 396
AciI CCGC 5 cut(s) 45, 57, 76, 483, 506
AclWI GGATC 2 cut(s) 227, 869
AcoI YGGCCR 1 cut(s) 679
AcsI RAATTY 2 cut(s) 649, 852
AfaI GTAC 4 cut(s) 118, 393, 497, 963
AfiI CCNNNNNNNGG 1 cut(s) 367
AgsI TTSAA 4 cut(s) 319, 692, 893, 973
AhlI ACTAGT 3 cut(s) 86, 113, 239
AjiI CACGTC 1 cut(s) 488
AluBI AGCT 6 cut(s) 125, 331, 380, 409, 719, 967
AluI AGCT 6 cut(s) 125, 331, 380, 409, 719, 967
Alw21I GWGCWC 2 cut(s) 254, 526
Alw26I GTCTC 1 cut(s) 496
Alw44I GTGCAC 1 cut(s) 522
AlwI GGATC 2 cut(s) 227, 869
AoxI GGCC 2 cut(s) 294, 679
ApaLI GTGCAC 1 cut(s) 522
ApeKI GCWGC 4 cut(s) 47, 50, 53, 122
ApoI RAATTY 2 cut(s) 649, 852
Asp700I GAANNNNTTC 1 cut(s) 649
AspS9I GGNCC 1 cut(s) 463
AsuHPI GGTGA 3 cut(s) 94, 133, 884
AvaII GGWCC 1 cut(s) 463
BaeGI GKGCMC 2 cut(s) 430, 526
BalI TGGCCA 1 cut(s) 681
BanI GGYRCC 1 cut(s) 65
BbsI GAAGAC 1 cut(s) 980
Bbv12I GWGCWC 2 cut(s) 254, 526
BbvI GCAGC 4 cut(s) 34, 37, 40, 134
BccI CCATC 3 cut(s) 26, 305, 896
BceAI ACGGC 1 cut(s) 744
BclI TGATCA 1 cut(s) 636
BcoDI GTCTC 1 cut(s) 496
BcuI ACTAGT 3 cut(s) 86, 113, 239
BfaI CTAG 5 cut(s) 87, 114, 240, 527, 831
BfmI CTRYAG 2 cut(s) 120, 375
BisI GCNGC 5 cut(s) 48, 51, 54, 57, 123
BlsI GCNGC 5 cut(s) 49, 52, 55, 58, 124
BmcAI AGTACT 1 cut(s) 118
Bme18I GGWCC 1 cut(s) 463
BmgBI CACGTC 1 cut(s) 488
BmgT120I GGNCC 1 cut(s) 463
BmiI GGNNCC 3 cut(s) 67, 436, 465
BmrI ACTGGG 2 cut(s) 461, 649
BmuI ACTGGG 2 cut(s) 461, 649
BoxI GACNNNNGTC 1 cut(s) 575
BpiI GAAGAC 1 cut(s) 980
BpuEI CTTGAG 1 cut(s) 425
BsaI GGTCTC 1 cut(s) 496
BsaJI CCNNGG 2 cut(s) 363, 430
Bsc4I CCNNNNNNNGG 1 cut(s) 367
Bse118I RCCGGY 1 cut(s) 62
Bse1I ACTGG 4 cut(s) 467, 519, 655, 951
Bse3DI GCAATG 2 cut(s) 343, 729
BseDI CCNNGG 2 cut(s) 363, 430
BseGI GGATG 1 cut(s) 904
BseLI CCNNNNNNNGG 1 cut(s) 367
BseMI GCAATG 2 cut(s) 343, 729
BseMII CTCAG 1 cut(s) 572
BseNI ACTGG 4 cut(s) 467, 519, 655, 951
BseRI GAGGAG 4 cut(s) 169, 178, 181, 642
BseSI GKGCMC 2 cut(s) 430, 526
BseXI GCAGC 4 cut(s) 34, 37, 40, 134
Bsh1236I CGCG 1 cut(s) 396
BshFI GGCC 2 cut(s) 296, 681
BshNI GGYRCC 1 cut(s) 65
BsiHKAI GWGCWC 2 cut(s) 254, 526
BsiSI CCGG 1 cut(s) 63
BslI CCNNNNNNNGG 1 cut(s) 367
BsmAI GTCTC 1 cut(s) 496
BsnI GGCC 2 cut(s) 296, 681
Bso31I GGTCTC 1 cut(s) 496
Bsp1286I GDGCHC 3 cut(s) 254, 430, 526
Bsp1407I TGTACA 1 cut(s) 961
Bsp143I GATC 4 cut(s) 232, 636, 799, 874
BspACI CCGC 5 cut(s) 45, 57, 76, 483, 506
BspANI GGCC 2 cut(s) 296, 681
BspCNI CTCAG 1 cut(s) 573
BspFNI CGCG 1 cut(s) 396
BspLI GGNNCC 3 cut(s) 67, 436, 465
BspMAI CTGCAG 1 cut(s) 124
BspPI GGATC 2 cut(s) 227, 869
BspT107I GGYRCC 1 cut(s) 65
BspTNI GGTCTC 1 cut(s) 496
BsrDI GCAATG 2 cut(s) 343, 729
BsrFI RCCGGY 1 cut(s) 62
BsrGI TGTACA 1 cut(s) 961
BsrI ACTGG 4 cut(s) 467, 519, 655, 951
BssAI RCCGGY 1 cut(s) 62
BssECI CCNNGG 2 cut(s) 363, 430
BssMI GATC 4 cut(s) 232, 636, 799, 874
BssNAI GTATAC 1 cut(s) 922
BssT1I CCWWGG 2 cut(s) 363, 430
Bst1107I GTATAC 1 cut(s) 922
Bst4CI ACNGT 2 cut(s) 872, 936
Bst6I CTCTTC 2 cut(s) 187, 693
BstAUI TGTACA 1 cut(s) 961
BstDEI CTNAG 1 cut(s) 581
BstF5I GGATG 1 cut(s) 904
BstFNI CGCG 1 cut(s) 396
BstKTI GATC 4 cut(s) 235, 639, 802, 877
BstMAI GTCTC 1 cut(s) 496
BstMBI GATC 4 cut(s) 232, 636, 799, 874
BstMWI GCNNNNNNNGC 5 cut(s) 32, 53, 56, 357, 725
BstNSI RCATGY 1 cut(s) 565
BstPAI GACNNNNGTC 1 cut(s) 575
BstSFI CTRYAG 2 cut(s) 120, 375
BstSLI GKGCMC 2 cut(s) 430, 526
BstUI CGCG 1 cut(s) 396
BstV1I GCAGC 4 cut(s) 34, 37, 40, 134
BstV2I GAAGAC 1 cut(s) 980
BstXI CCANNNNNNTGG 1 cut(s) 677
BstZ17I GTATAC 1 cut(s) 922
BsuRI GGCC 2 cut(s) 296, 681
BtrI CACGTC 1 cut(s) 488
BtsCI GGATG 1 cut(s) 904
BtsI GCAGTG 1 cut(s) 21
BtsIMutI CAGTG 3 cut(s) 21, 526, 944
Cfr10I RCCGGY 1 cut(s) 62
Cfr13I GGNCC 1 cut(s) 463
Csp6I GTAC 4 cut(s) 117, 392, 496, 962
CviAII CATG 3 cut(s) 562, 695, 847
CviQI GTAC 4 cut(s) 117, 392, 496, 962
DdeI CTNAG 1 cut(s) 581
DpnI GATC 4 cut(s) 234, 638, 801, 876
DpnII GATC 4 cut(s) 232, 636, 799, 874
EaeI YGGCCR 1 cut(s) 679
Eam1104I CTCTTC 2 cut(s) 187, 693
EarI CTCTTC 2 cut(s) 187, 693
Eco130I CCWWGG 2 cut(s) 363, 430
Eco31I GGTCTC 1 cut(s) 496
Eco32I GATATC 1 cut(s) 400
Eco47I GGWCC 1 cut(s) 463
EcoO109I RGGNCCY 1 cut(s) 463
EcoRV GATATC 1 cut(s) 400
EcoT14I CCWWGG 2 cut(s) 363, 430
ErhI CCWWGG 2 cut(s) 363, 430
FaeI CATG 3 cut(s) 565, 698, 850
FalI AAGNNNNNCTT 1 cut(s) 965
FatI CATG 3 cut(s) 561, 694, 846
FauI CCCGC 1 cut(s) 52
FbaI TGATCA 1 cut(s) 636
FblI GTMKAC 3 cut(s) 272, 374, 921
Fnu4HI GCNGC 5 cut(s) 48, 51, 54, 57, 123
FokI GGATG 1 cut(s) 911
Fsp4HI GCNGC 5 cut(s) 48, 51, 54, 57, 123
FspBI CTAG 5 cut(s) 87, 114, 240, 527, 831
GluI GCNGC 5 cut(s) 48, 51, 54, 57, 123
HaeIII GGCC 2 cut(s) 296, 681
HapII CCGG 1 cut(s) 63
Hin1II CATG 3 cut(s) 565, 698, 850
HincII GTYRAC 1 cut(s) 273
HindII GTYRAC 1 cut(s) 273
HinfI GANTC 2 cut(s) 260, 371
HpaII CCGG 1 cut(s) 63
HphI GGTGA 3 cut(s) 94, 133, 884
Hpy166II GTNNAC 6 cut(s) 273, 375, 524, 594, 660, 922
Hpy188I TCNGA 2 cut(s) 237, 265
Hpy188III TCNNGA 4 cut(s) 580, 748, 860, 910
Hpy8I GTNNAC 6 cut(s) 273, 375, 524, 594, 660, 922
Hpy99I CGWCG 3 cut(s) 277, 280, 574
HpyAV CCTTC 3 cut(s) 220, 410, 601
HpyCH4III ACNGT 2 cut(s) 872, 936
HpyCH4IV ACGT 1 cut(s) 487
HpyCH4V TGCA 5 cut(s) 122, 336, 351, 524, 550
HpyF10VI GCNNNNNNNGC 5 cut(s) 32, 53, 56, 357, 725
HpyF3I CTNAG 1 cut(s) 581
HpySE526I ACGT 1 cut(s) 487
Hsp92II CATG 3 cut(s) 565, 698, 850
Ksp22I TGATCA 1 cut(s) 636
Kzo9I GATC 4 cut(s) 232, 636, 799, 874
LmnI GCTCC 2 cut(s) 249, 328
Lsp1109I GCAGC 4 cut(s) 34, 37, 40, 134
MaeI CTAG 5 cut(s) 87, 114, 240, 527, 831
MaeII ACGT 1 cut(s) 487
MaeIII GTNAC 2 cut(s) 223, 488
MalI GATC 4 cut(s) 234, 638, 801, 876
MboI GATC 4 cut(s) 232, 636, 799, 874
MboII GAAGA 4 cut(s) 174, 625, 710, 985
MfeI CAATTG 1 cut(s) 774
MhlI GDGCHC 3 cut(s) 254, 430, 526
MlsI TGGCCA 1 cut(s) 681
MluCI AATT 8 cut(s) 135, 154, 166, 266, 649, 774, 852, 927
MluNI TGGCCA 1 cut(s) 681
MlyI GAGTC 1 cut(s) 380
MmeI TCCRAC 2 cut(s) 260, 902
Mox20I TGGCCA 1 cut(s) 681
MroXI GAANNNNTTC 1 cut(s) 649
MscI TGGCCA 1 cut(s) 681
MseI TTAA 1 cut(s) 453
Msp20I TGGCCA 1 cut(s) 681
MspA1I CMGCKG 1 cut(s) 47
MspI CCGG 1 cut(s) 63
MunI CAATTG 1 cut(s) 774
MvnI CGCG 1 cut(s) 396
MwoI GCNNNNNNNGC 5 cut(s) 32, 53, 56, 357, 725
NdeII GATC 4 cut(s) 232, 636, 799, 874
NlaIII CATG 3 cut(s) 565, 698, 850
NlaIV GGNNCC 3 cut(s) 67, 436, 465
NmuCI GTSAC 1 cut(s) 488
NspI RCATGY 1 cut(s) 565
PdmI GAANNNNTTC 1 cut(s) 649
PfeI GAWTC 1 cut(s) 260
PkrI GCNGC 5 cut(s) 49, 52, 55, 58, 124
PleI GAGTC 1 cut(s) 379
PpsI GAGTC 1 cut(s) 379
PpuMI RGGWCCY 1 cut(s) 463
PshAI GACNNNNGTC 1 cut(s) 575
Psp5II RGGWCCY 1 cut(s) 463
PspN4I GGNNCC 3 cut(s) 67, 436, 465
PspPI GGNCC 1 cut(s) 463
PspPPI RGGWCCY 1 cut(s) 463
PstI CTGCAG 1 cut(s) 124
RsaI GTAC 4 cut(s) 118, 393, 497, 963
RsaNI GTAC 4 cut(s) 117, 392, 496, 962
SalI GTCGAC 1 cut(s) 271
SaqAI TTAA 1 cut(s) 453
SatI GCNGC 5 cut(s) 48, 51, 54, 57, 123
Sau3AI GATC 4 cut(s) 232, 636, 799, 874
Sau96I GGNCC 1 cut(s) 463
ScaI AGTACT 1 cut(s) 118
SchI GAGTC 1 cut(s) 380
SduI GDGCHC 3 cut(s) 254, 430, 526
SfcI CTRYAG 2 cut(s) 120, 375
SinI GGWCC 1 cut(s) 463
SmlI CTYRAG 1 cut(s) 404
SmoI CTYRAG 1 cut(s) 404
SpeI ACTAGT 3 cut(s) 86, 113, 239
Sse9I AATT 8 cut(s) 135, 154, 166, 266, 649, 774, 852, 927
SsiI CCGC 5 cut(s) 45, 57, 76, 483, 506
SspMI CTAG 5 cut(s) 87, 114, 240, 527, 831
StyI CCWWGG 2 cut(s) 363, 430
TaaI ACNGT 2 cut(s) 872, 936
TaiI ACGT 1 cut(s) 490
TaqI TCGA 3 cut(s) 272, 382, 569
TasI AATT 8 cut(s) 135, 154, 166, 266, 649, 774, 852, 927
TatI WGTACW 3 cut(s) 116, 495, 961
TauI GCSGC 1 cut(s) 59
TfiI GAWTC 1 cut(s) 260
Tru1I TTAA 1 cut(s) 453
Tru9I TTAA 1 cut(s) 453
TscAI CASTG 3 cut(s) 28, 526, 951
TseFI GTSAC 1 cut(s) 488
TseI GCWGC 4 cut(s) 47, 50, 53, 122
Tsp45I GTSAC 1 cut(s) 488
TspDTI ATGAA 3 cut(s) 202, 711, 711
TspGWI ACGGA 2 cut(s) 293, 305
TspRI CASTG 3 cut(s) 28, 526, 951
VneI GTGCAC 1 cut(s) 522
VpaK11BI GGWCC 1 cut(s) 463
XapI RAATTY 2 cut(s) 649, 852
XceI RCATGY 1 cut(s) 565
XmiI GTMKAC 3 cut(s) 272, 374, 921
XmnI GAANNNNTTC 1 cut(s) 649
XspI CTAG 5 cut(s) 87, 114, 240, 527, 831
ZrmI AGTACT 1 cut(s) 118
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.