AT1G65270

ER membrane protein complex

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0015492
Physical Location & Seq
Forward (+)
24241706 .. 24245074
3369 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G65270.1

Sequence Viewer

Length: 879 bp
ATGGCGAAGCTCACACTTCTCTTCTTCCTTTCCTTTCTCATCTTCTCCTCATCCATCGCTTTCCAATCAGACGAGCTCCTCGTTGACGACGACGAATTTGGTCTAGAAGGAGCAAAGCCCCGCTCCACCGATCTTTACACATCTTCTTCTTCTTCGCCACAGCAGCAGCAACAGACTCCAACTATCCGGAGAAGATACTCAGATCCTACCGATTTGGATTCAAAAGTCCAATTTACTCTCGAACATGCCTTCGGTGACTCTGATTTCTCCCCCGCCGGTACTTTCTCCGCTCGTCTCAAAACCTGGAGTCATGGCGGAAAGACATTAACGAAGCTGCGATTCTCTAGAAATGATTTTTCTGCTGAAGAGAAAGATGCATTCAAGAATCTGCTGAAAGGAGATGACTTTTATCGGATTCGGCTTCCATCTAATGTGGTTAGTCCACCAGGGAGAGAGTTTGTGATTGCATCAGTGAGAGCTAGATGTCTACCACGGGATGGCTTGGATGAGCATTTCATTATACACATGGAAGGTGCTAACATCTTGGCAGTTAGTTATGGTTCTCCTGGGGCGTGTCAATATCCTCGCCAATTGAAACTTCCAGCAAAATGGTCGTTTAACTCTCACACAATTCTGAAAAGCAGTGAGCAGGCGCCAAGAACTCCAATATTCACTGAGGAGATTCTAGGCAGTGAGAATGTAGAGGGAGAAGTTGAACCACCACCAGAGAGATCATTTTGGGCGAAATATTGGATGTATTTGATACCGCTGGGACTCGTAGTGATGAATGCCGTGACACAAGCATCAAACATGGCTGAAGAACCTGCGGGTGGACAGGCAGGAGGTGCACAAGTGCAACCAGCTGCCAGGAGAAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

292

Amino Acids

32.53

Weight (kDa)

6.01

Isoelectric Point (pI)

55.2

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015492)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G65270 AT1G65270 AT1G65270
fragaria_vesca FvH4_6g00240
malus_domestica MD04G1246900.v1.1 MD12G1263300.v1.1
prunus_persica Prupe.6G363100_v2.0.a1
pyrus_communis pycom04g21760 pycom12g24060
rosa_chinensis RchiOBHm_Chr3g0447611
rosa_laevigata RLG00000025984
rosa_multiflora Rmu_sc0003550.1_g000004
rosa_roxburghii Rroxscaffold_6G00425820
rosa_rugosa Rorug02G0603500
rosa_samantha Rh3AG003700
rosa_wichuraiana Rw3G000260

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 832
AccB1I GGYRCC 1 cut(s) 652
AccB7I CCANNNNNTGG 1 cut(s) 497
AccBSI CCGCTC 2 cut(s) 123, 290
AccI GTMKAC 1 cut(s) 487
AccIII TCCGGA 1 cut(s) 186
AciI CCGC 6 cut(s) 121, 273, 288, 315, 767, 827
AclWI GGATC 1 cut(s) 197
AcsI RAATTY 1 cut(s) 95
AcuI CTGAAG 2 cut(s) 384, 837
AcyI GRCGYC 1 cut(s) 653
AfaI GTAC 1 cut(s) 280
AfiI CCNNNNNNNGG 2 cut(s) 497, 830
AgsI TTSAA 4 cut(s) 222, 382, 595, 716
AjnI CCWGG 4 cut(s) 302, 445, 565, 866
AjuI GAANNNNNNNTTGG 2 cut(s) 582, 614
AluBI AGCT 5 cut(s) 10, 76, 334, 479, 863
AluI AGCT 5 cut(s) 10, 76, 334, 479, 863
Alw21I GWGCWC 2 cut(s) 78, 850
Alw26I GTCTC 1 cut(s) 299
Alw44I GTGCAC 1 cut(s) 846
AlwI GGATC 1 cut(s) 197
Aor13HI TCCGGA 1 cut(s) 186
ApaLI GTGCAC 1 cut(s) 846
ApeKI GCWGC 4 cut(s) 163, 166, 334, 863
ApoI RAATTY 1 cut(s) 95
ArsI GACNNNNNNTTYG 2 cut(s) 80, 112
AspLEI GCGC 1 cut(s) 655
AsuHPI GGTGA 1 cut(s) 266
BaeGI GKGCMC 1 cut(s) 850
BanI GGYRCC 1 cut(s) 652
BanII GRGCYC 1 cut(s) 78
Bbv12I GWGCWC 2 cut(s) 78, 850
BbvI GCAGC 4 cut(s) 175, 178, 321, 850
BccI CCATC 3 cut(s) 62, 433, 491
BceAI ACGGC 1 cut(s) 776
BcgI CGANNNNNNTGC 2 cut(s) 594, 628
BciT130I CCWGG 4 cut(s) 304, 447, 567, 868
BcoDI GTCTC 1 cut(s) 299
BfaI CTAG 4 cut(s) 104, 345, 480, 686
BfoI RGCGCY 1 cut(s) 656
BfuAI ACCTGC 1 cut(s) 832
BisI GCNGC 4 cut(s) 164, 167, 335, 864
BlsI GCNGC 4 cut(s) 165, 168, 336, 865
Bme1390I CCNGG 4 cut(s) 304, 447, 567, 868
BmiI GGNNCC 1 cut(s) 654
BmrFI CCNGG 4 cut(s) 304, 447, 567, 868
BmsI GCATC 3 cut(s) 364, 476, 812
BpmI CTGGAG 1 cut(s) 325
BsaHI GRCGYC 1 cut(s) 653
BsaJI CCNNGG 3 cut(s) 446, 491, 566
BsaWI WCCGGW 1 cut(s) 186
Bsc4I CCNNNNNNNGG 2 cut(s) 497, 830
Bse118I RCCGGY 1 cut(s) 275
BseAI TCCGGA 1 cut(s) 186
BseBI CCWGG 4 cut(s) 304, 447, 567, 868
BseDI CCNNGG 3 cut(s) 446, 491, 566
BseGI GGATG 4 cut(s) 50, 502, 511, 759
BseLI CCNNNNNNNGG 2 cut(s) 497, 830
BseMII CTCAG 2 cut(s) 213, 666
BseRI GAGGAG 3 cut(s) 37, 68, 692
BseSI GKGCMC 1 cut(s) 850
BseXI GCAGC 4 cut(s) 175, 178, 321, 850
BseYI CCCAGC 1 cut(s) 769
BshNI GGYRCC 1 cut(s) 652
BsiHKAI GWGCWC 2 cut(s) 78, 850
BsiSI CCGG 2 cut(s) 187, 276
BslFI GGGAC 1 cut(s) 786
BslI CCNNNNNNNGG 2 cut(s) 497, 830
BsmAI GTCTC 1 cut(s) 299
BsmBI CGTCTC 1 cut(s) 299
BsmFI GGGAC 1 cut(s) 786
BsmI GAATGC 2 cut(s) 377, 793
Bsp1286I GDGCHC 2 cut(s) 78, 850
Bsp13I TCCGGA 1 cut(s) 186
Bsp143I GATC 3 cut(s) 130, 202, 731
BspACI CCGC 6 cut(s) 121, 273, 288, 315, 767, 827
BspCNI CTCAG 2 cut(s) 212, 667
BspEI TCCGGA 1 cut(s) 186
BspLI GGNNCC 1 cut(s) 654
BspMI ACCTGC 1 cut(s) 832
BspPI GGATC 1 cut(s) 197
BspT107I GGYRCC 1 cut(s) 652
BsrBI CCGCTC 2 cut(s) 123, 290
BsrFI RCCGGY 1 cut(s) 275
BssAI RCCGGY 1 cut(s) 275
BssECI CCNNGG 3 cut(s) 446, 491, 566
BssMI GATC 3 cut(s) 130, 202, 731
BssNI GRCGYC 1 cut(s) 653
Bst2UI CCWGG 4 cut(s) 304, 447, 567, 868
Bst6I CTCTTC 2 cut(s) 26, 360
BstACI GRCGYC 1 cut(s) 653
BstAPI GCANNNNNTGC 1 cut(s) 845
BstC8I GCNNGC 1 cut(s) 651
BstDEI CTNAG 2 cut(s) 199, 675
BstDSI CCRYGG 1 cut(s) 491
BstF5I GGATG 4 cut(s) 50, 502, 511, 759
BstH2I RGCGCY 1 cut(s) 656
BstHHI GCGC 1 cut(s) 655
BstKTI GATC 3 cut(s) 133, 205, 734
BstMAI GTCTC 1 cut(s) 299
BstMBI GATC 3 cut(s) 130, 202, 731
BstMWI GCNNNNNNNGC 2 cut(s) 163, 845
BstNI CCWGG 4 cut(s) 304, 447, 567, 868
BstNSI RCATGY 1 cut(s) 248
BstSCI CCNGG 4 cut(s) 302, 445, 565, 866
BstSLI GKGCMC 1 cut(s) 850
BstV1I GCAGC 4 cut(s) 175, 178, 321, 850
BstX2I RGATCY 1 cut(s) 202
BstXI CCANNNNNNTGG 1 cut(s) 609
BstYI RGATCY 1 cut(s) 202
BtgI CCRYGG 1 cut(s) 491
BtgZI GCGATG 1 cut(s) 40
BtsCI GGATG 4 cut(s) 50, 502, 511, 759
BtsI GCAGTG 2 cut(s) 649, 697
BtsIMutI CAGTG 4 cut(s) 477, 649, 672, 697
BveI ACCTGC 1 cut(s) 832
Cac8I GCNNGC 1 cut(s) 651
CfoI GCGC 1 cut(s) 655
Cfr10I RCCGGY 1 cut(s) 275
Csp6I GTAC 1 cut(s) 279
CviAII CATG 4 cut(s) 245, 311, 526, 811
CviJI RGCY 9 cut(s) 10, 76, 118, 334, 421, 479, 501, 815, 863
CviKI_1 RGCY 9 cut(s) 10, 76, 118, 334, 421, 479, 501, 815, 863
CviQI GTAC 1 cut(s) 279
DdeI CTNAG 2 cut(s) 199, 675
DinI GGCGCC 1 cut(s) 654
DpnI GATC 3 cut(s) 132, 204, 733
DpnII GATC 3 cut(s) 130, 202, 731
Eam1104I CTCTTC 2 cut(s) 26, 360
EarI CTCTTC 2 cut(s) 26, 360
EciI GGCGGA 1 cut(s) 330
Ecl136II GAGCTC 1 cut(s) 76
Eco24I GRGCYC 1 cut(s) 78
Eco53kI GAGCTC 1 cut(s) 76
Eco57I CTGAAG 2 cut(s) 384, 837
EcoICRI GAGCTC 1 cut(s) 76
EcoRII CCWGG 4 cut(s) 302, 445, 565, 866
EcoT22I ATGCAT 1 cut(s) 379
EcoT38I GRGCYC 1 cut(s) 78
EgeI GGCGCC 1 cut(s) 654
EheI GGCGCC 1 cut(s) 654
Esp3I CGTCTC 1 cut(s) 299
FaeI CATG 4 cut(s) 248, 314, 529, 814
FaiI YATR 6 cut(s) 246, 312, 521, 527, 558, 812
FaqI GGGAC 1 cut(s) 786
FatI CATG 4 cut(s) 244, 310, 525, 810
FauI CCCGC 3 cut(s) 128, 280, 820
FblI GTMKAC 1 cut(s) 487
Fnu4HI GCNGC 4 cut(s) 164, 167, 335, 864
FokI GGATG 4 cut(s) 37, 509, 518, 766
FriOI GRGCYC 1 cut(s) 78
Fsp4HI GCNGC 4 cut(s) 164, 167, 335, 864
FspBI CTAG 4 cut(s) 104, 345, 480, 686
GlaI GCGC 1 cut(s) 654
GluI GCNGC 4 cut(s) 164, 167, 335, 864
GsaI CCCAGC 1 cut(s) 773
GsuI CTGGAG 1 cut(s) 325
HaeII RGCGCY 1 cut(s) 656
HapII CCGG 2 cut(s) 187, 276
HhaI GCGC 1 cut(s) 655
Hin1I GRCGYC 1 cut(s) 653
Hin1II CATG 4 cut(s) 248, 314, 529, 814
Hin6I GCGC 1 cut(s) 653
HinP1I GCGC 1 cut(s) 653
HincII GTYRAC 1 cut(s) 85
HindII GTYRAC 1 cut(s) 85
HinfI GANTC 9 cut(s) 175, 218, 257, 307, 339, 385, 415, 682, 774
HpaII CCGG 2 cut(s) 187, 276
HphI GGTGA 1 cut(s) 266
Hpy166II GTNNAC 5 cut(s) 85, 443, 488, 833, 848
Hpy188I TCNGA 5 cut(s) 70, 202, 262, 414, 636
Hpy188III TCNNGA 5 cut(s) 104, 187, 239, 345, 382
Hpy8I GTNNAC 5 cut(s) 85, 443, 488, 833, 848
Hpy99I CGWCG 2 cut(s) 92, 95
HpyAV CCTTC 3 cut(s) 101, 259, 524
HpyCH4V TGCA 4 cut(s) 377, 467, 848, 856
HpyF10VI GCNNNNNNNGC 2 cut(s) 163, 845
HpyF3I CTNAG 2 cut(s) 199, 675
Hsp92I GRCGYC 1 cut(s) 653
Hsp92II CATG 4 cut(s) 248, 314, 529, 814
HspAI GCGC 1 cut(s) 653
KasI GGCGCC 1 cut(s) 652
Kpn2I TCCGGA 1 cut(s) 186
Kzo9I GATC 3 cut(s) 130, 202, 731
LmnI GCTCC 3 cut(s) 81, 110, 128
Lsp1109I GCAGC 4 cut(s) 175, 178, 321, 850
LweI GCATC 3 cut(s) 364, 476, 812
MaeI CTAG 4 cut(s) 104, 345, 480, 686
MaeIII GTNAC 2 cut(s) 254, 793
MalI GATC 3 cut(s) 132, 204, 733
MbiI CCGCTC 2 cut(s) 123, 290
MboI GATC 3 cut(s) 130, 202, 731
MfeI CAATTG 1 cut(s) 590
MflI RGATCY 1 cut(s) 202
MhlI GDGCHC 2 cut(s) 78, 850
MluCI AATT 4 cut(s) 95, 230, 590, 630
Mly113I GGCGCC 1 cut(s) 653
MlyI GAGTC 4 cut(s) 169, 251, 316, 768
MmeI TCCRAC 1 cut(s) 203
MnlI CCTC 6 cut(s) 58, 89, 594, 670, 697, 836
Mph1103I ATGCAT 1 cut(s) 379
MroI TCCGGA 1 cut(s) 186
MseI TTAA 2 cut(s) 326, 618
MspA1I CMGCKG 2 cut(s) 769, 863
MspI CCGG 2 cut(s) 187, 276
MspR9I CCNGG 4 cut(s) 304, 447, 567, 868
MunI CAATTG 1 cut(s) 590
Mva1269I GAATGC 2 cut(s) 377, 793
MvaI CCWGG 4 cut(s) 304, 447, 567, 868
MwoI GCNNNNNNNGC 2 cut(s) 163, 845
NarI GGCGCC 1 cut(s) 653
NdeII GATC 3 cut(s) 130, 202, 731
NlaIII CATG 4 cut(s) 248, 314, 529, 814
NlaIV GGNNCC 1 cut(s) 654
NmuCI GTSAC 2 cut(s) 254, 793
NsiI ATGCAT 1 cut(s) 379
NspI RCATGY 1 cut(s) 248
PcsI WCGNNNNNNNCGW 2 cut(s) 78, 87
PctI GAATGC 2 cut(s) 377, 793
PfeI GAWTC 5 cut(s) 218, 339, 385, 415, 682
PflMI CCANNNNNTGG 1 cut(s) 497
PkrI GCNGC 4 cut(s) 165, 168, 336, 865
PleI GAGTC 4 cut(s) 169, 251, 315, 768
PluTI GGCGCC 1 cut(s) 656
PpsI GAGTC 4 cut(s) 169, 251, 315, 768
Psp124BI GAGCTC 1 cut(s) 78
Psp6I CCWGG 4 cut(s) 302, 445, 565, 866
PspFI CCCAGC 1 cut(s) 769
PspGI CCWGG 4 cut(s) 302, 445, 565, 866
PspN4I GGNNCC 1 cut(s) 654
PsuI RGATCY 1 cut(s) 202
PvuII CAGCTG 1 cut(s) 863
RsaI GTAC 1 cut(s) 280
RsaNI GTAC 1 cut(s) 279
SacI GAGCTC 1 cut(s) 78
SaqAI TTAA 2 cut(s) 326, 618
SatI GCNGC 4 cut(s) 164, 167, 335, 864
Sau3AI GATC 3 cut(s) 130, 202, 731
SchI GAGTC 4 cut(s) 169, 251, 316, 768
ScrFI CCNGG 4 cut(s) 304, 447, 567, 868
SduI GDGCHC 2 cut(s) 78, 850
SetI ASST 9 cut(s) 12, 78, 305, 336, 481, 535, 826, 847, 865
SfaNI GCATC 3 cut(s) 364, 476, 812
SfoI GGCGCC 1 cut(s) 654
Sse9I AATT 4 cut(s) 95, 230, 590, 630
SsiI CCGC 6 cut(s) 121, 273, 288, 315, 767, 827
SspDI GGCGCC 1 cut(s) 652
SspI AATATT 2 cut(s) 669, 749
SspMI CTAG 4 cut(s) 104, 345, 480, 686
SstI GAGCTC 1 cut(s) 78
StyD4I CCNGG 4 cut(s) 302, 445, 565, 866
TaqI TCGA 1 cut(s) 240
TasI AATT 4 cut(s) 95, 230, 590, 630
TfiI GAWTC 5 cut(s) 218, 339, 385, 415, 682
Tru1I TTAA 2 cut(s) 326, 618
Tru9I TTAA 2 cut(s) 326, 618
TscAI CASTG 4 cut(s) 477, 649, 679, 697
TseFI GTSAC 2 cut(s) 254, 793
TseI GCWGC 4 cut(s) 163, 166, 334, 863
Tsp45I GTSAC 2 cut(s) 254, 793
TspDTI ATGAA 2 cut(s) 505, 800
TspRI CASTG 4 cut(s) 477, 649, 679, 697
Van91I CCANNNNNTGG 1 cut(s) 497
VneI GTGCAC 1 cut(s) 846
XapI RAATTY 1 cut(s) 95
XbaI TCTAGA 2 cut(s) 103, 344
XceI RCATGY 1 cut(s) 248
XmiI GTMKAC 1 cut(s) 487
XspI CTAG 4 cut(s) 104, 345, 480, 686
Zsp2I ATGCAT 1 cut(s) 379
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.