pycom12g24060

ER membrane protein complex subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0015492
Physical Location & Seq
Forward (+)
24451582 .. 24452539
958 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g24060.3

Sequence Viewer

Length: 318 bp
ATGAAACTTCCTGGAAAATGGTCCTTTAACTCTCACACGGTTTTGAAGAATAGTGAGCAGGCACCAAGAGCTCCAGTGTTTGCTGAGGATATTCTTGGGGTTGGGGGCGAGGTAGGAGAAGGTGAAATTATACCACCACCAGAAAGGTCTTTTTGGGCTAAATATTGGATGTACGCGATCCCTCTTGGGCTCATTGTCATGAATGCCATTACCCAAGCAATGAACATGGCGGAGGAGGGTGCTGGTCAGCCAGCTGGCCAAGCTCAGCAGCCAGCTGCAGTCCAGCGTGGGTCAAGTTCTGCTGTGCGAAGGCGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

106

Amino Acids

11.34

Weight (kDa)

8.12

Isoelectric Point (pI)

58.15

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015492)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G65270 AT1G65270 AT1G65270
fragaria_vesca FvH4_6g00240
malus_domestica MD04G1246900.v1.1 MD12G1263300.v1.1
prunus_persica Prupe.6G363100_v2.0.a1
pyrus_communis pycom04g21760 pycom12g24060
rosa_chinensis RchiOBHm_Chr3g0447611
rosa_laevigata RLG00000025984
rosa_multiflora Rmu_sc0003550.1_g000004
rosa_roxburghii Rroxscaffold_6G00425820
rosa_rugosa Rorug02G0603500
rosa_samantha Rh3AG003700
rosa_wichuraiana Rw3G000260

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 61
AccII CGCG 1 cut(s) 176
AciI CCGC 1 cut(s) 230
AclWI GGATC 1 cut(s) 172
AcoI YGGCCR 1 cut(s) 256
AfaI GTAC 1 cut(s) 173
AgsI TTSAA 1 cut(s) 46
AjnI CCWGG 1 cut(s) 10
AluBI AGCT 4 cut(s) 71, 254, 263, 275
AluI AGCT 4 cut(s) 71, 254, 263, 275
Alw21I GWGCWC 1 cut(s) 73
AlwI GGATC 1 cut(s) 172
AoxI GGCC 1 cut(s) 256
ApeKI GCWGC 2 cut(s) 268, 275
AspS9I GGNCC 1 cut(s) 21
AsuHPI GGTGA 1 cut(s) 134
AvaII GGWCC 1 cut(s) 21
BalI TGGCCA 1 cut(s) 258
BanI GGYRCC 1 cut(s) 61
BanII GRGCYC 2 cut(s) 73, 192
Bbv12I GWGCWC 1 cut(s) 73
BbvCI CCTCAGC 1 cut(s) 84
BbvI GCAGC 2 cut(s) 262, 280
BciT130I CCWGG 1 cut(s) 12
BfmI CTRYAG 1 cut(s) 276
BisI GCNGC 2 cut(s) 269, 276
BlpI GCTNAGC 1 cut(s) 264
BlsI GCNGC 2 cut(s) 270, 277
Bme1390I CCNGG 1 cut(s) 12
Bme18I GGWCC 1 cut(s) 21
BmgT120I GGNCC 1 cut(s) 21
BmiI GGNNCC 1 cut(s) 63
BmrFI CCNGG 1 cut(s) 12
BpmI CTGGAG 1 cut(s) 57
Bpu10I CCTNAGC 1 cut(s) 84
Bpu1102I GCTNAGC 1 cut(s) 264
Bse1I ACTGG 1 cut(s) 74
Bse3DI GCAATG 1 cut(s) 225
BseBI CCWGG 1 cut(s) 12
BseGI GGATG 1 cut(s) 174
BseMI GCAATG 1 cut(s) 225
BseMII CTCAG 2 cut(s) 75, 278
BseNI ACTGG 1 cut(s) 74
BseRI GAGGAG 1 cut(s) 248
BseXI GCAGC 2 cut(s) 262, 280
Bsh1236I CGCG 1 cut(s) 176
BshFI GGCC 1 cut(s) 258
BshNI GGYRCC 1 cut(s) 61
BsiHKAI GWGCWC 1 cut(s) 73
BsmI GAATGC 1 cut(s) 208
BsnI GGCC 1 cut(s) 258
Bsp1286I GDGCHC 2 cut(s) 73, 192
Bsp143I GATC 1 cut(s) 177
Bsp1720I GCTNAGC 1 cut(s) 264
BspACI CCGC 1 cut(s) 230
BspANI GGCC 1 cut(s) 258
BspCNI CTCAG 2 cut(s) 76, 277
BspFNI CGCG 1 cut(s) 176
BspHI TCATGA 1 cut(s) 198
BspLI GGNNCC 1 cut(s) 63
BspMAI CTGCAG 1 cut(s) 280
BspPI GGATC 1 cut(s) 172
BspT107I GGYRCC 1 cut(s) 61
BsrDI GCAATG 1 cut(s) 225
BsrI ACTGG 1 cut(s) 74
BssMI GATC 1 cut(s) 177
Bst2UI CCWGG 1 cut(s) 12
Bst4CI ACNGT 1 cut(s) 40
BstC8I GCNNGC 4 cut(s) 60, 252, 256, 273
BstDEI CTNAG 2 cut(s) 84, 264
BstF5I GGATG 1 cut(s) 174
BstFNI CGCG 1 cut(s) 176
BstKTI GATC 1 cut(s) 180
BstMBI GATC 1 cut(s) 177
BstMWI GCNNNNNNNGC 2 cut(s) 68, 260
BstNI CCWGG 1 cut(s) 12
BstSCI CCNGG 1 cut(s) 10
BstSFI CTRYAG 1 cut(s) 276
BstUI CGCG 1 cut(s) 176
BstV1I GCAGC 2 cut(s) 262, 280
BsuRI GGCC 1 cut(s) 258
BtsCI GGATG 1 cut(s) 174
BtsIMutI CAGTG 1 cut(s) 81
Cac8I GCNNGC 4 cut(s) 60, 252, 256, 273
CciI TCATGA 1 cut(s) 198
Cfr13I GGNCC 1 cut(s) 21
Csp6I GTAC 1 cut(s) 172
CviAII CATG 2 cut(s) 199, 226
CviJI RGCY 9 cut(s) 71, 158, 190, 250, 254, 258, 263, 271, 275
CviKI_1 RGCY 9 cut(s) 71, 158, 190, 250, 254, 258, 263, 271, 275
CviQI GTAC 1 cut(s) 172
DdeI CTNAG 2 cut(s) 84, 264
DpnI GATC 1 cut(s) 179
DpnII GATC 1 cut(s) 177
EaeI YGGCCR 1 cut(s) 256
EciI GGCGGA 1 cut(s) 245
Ecl136II GAGCTC 1 cut(s) 71
Eco24I GRGCYC 2 cut(s) 73, 192
Eco47I GGWCC 1 cut(s) 21
Eco53kI GAGCTC 1 cut(s) 71
EcoICRI GAGCTC 1 cut(s) 71
EcoRII CCWGG 1 cut(s) 10
EcoT38I GRGCYC 2 cut(s) 73, 192
FaeI CATG 2 cut(s) 202, 229
FaiI YATR 3 cut(s) 131, 200, 227
FatI CATG 2 cut(s) 198, 225
Fnu4HI GCNGC 2 cut(s) 269, 276
FokI GGATG 1 cut(s) 181
FriOI GRGCYC 2 cut(s) 73, 192
Fsp4HI GCNGC 2 cut(s) 269, 276
GluI GCNGC 2 cut(s) 269, 276
GsuI CTGGAG 1 cut(s) 57
HaeIII GGCC 1 cut(s) 258
Hin1II CATG 2 cut(s) 202, 229
HphI GGTGA 1 cut(s) 134
Hpy188III TCNNGA 1 cut(s) 199
HpyAV CCTTC 2 cut(s) 113, 303
HpyCH4III ACNGT 1 cut(s) 40
HpyCH4V TGCA 1 cut(s) 278
HpyF10VI GCNNNNNNNGC 2 cut(s) 68, 260
HpyF3I CTNAG 2 cut(s) 84, 264
Hsp92II CATG 2 cut(s) 202, 229
Kzo9I GATC 1 cut(s) 177
LmnI GCTCC 1 cut(s) 76
LpnPI CCDG 9 cut(s) 24, 44, 87, 153, 228, 240, 264, 285, 296
Lsp1109I GCAGC 2 cut(s) 262, 280
MalI GATC 1 cut(s) 179
MboI GATC 1 cut(s) 177
MboII GAAGA 1 cut(s) 58
MhlI GDGCHC 2 cut(s) 73, 192
MlsI TGGCCA 1 cut(s) 258
MluCI AATT 1 cut(s) 126
MluNI TGGCCA 1 cut(s) 258
MnlI CCTC 5 cut(s) 79, 103, 192, 226, 229
Mox20I TGGCCA 1 cut(s) 258
MscI TGGCCA 1 cut(s) 258
MseI TTAA 1 cut(s) 27
MslI CAYNNNNRTG 1 cut(s) 197
Msp20I TGGCCA 1 cut(s) 258
MspA1I CMGCKG 2 cut(s) 254, 275
MspR9I CCNGG 1 cut(s) 12
Mva1269I GAATGC 1 cut(s) 208
MvaI CCWGG 1 cut(s) 12
MvnI CGCG 1 cut(s) 176
MwoI GCNNNNNNNGC 2 cut(s) 68, 260
NdeII GATC 1 cut(s) 177
NlaIII CATG 2 cut(s) 202, 229
NlaIV GGNNCC 1 cut(s) 63
PagI TCATGA 1 cut(s) 198
PctI GAATGC 1 cut(s) 208
PfoI TCCNGGA 1 cut(s) 10
PkrI GCNGC 2 cut(s) 270, 277
Psp124BI GAGCTC 1 cut(s) 73
Psp6I CCWGG 1 cut(s) 10
PspGI CCWGG 1 cut(s) 10
PspN4I GGNNCC 1 cut(s) 63
PspPI GGNCC 1 cut(s) 21
PstI CTGCAG 1 cut(s) 280
PvuII CAGCTG 2 cut(s) 254, 275
RsaI GTAC 1 cut(s) 173
RsaNI GTAC 1 cut(s) 172
RseI CAYNNNNRTG 1 cut(s) 197
SacI GAGCTC 1 cut(s) 73
SaqAI TTAA 1 cut(s) 27
SatI GCNGC 2 cut(s) 269, 276
Sau3AI GATC 1 cut(s) 177
Sau96I GGNCC 1 cut(s) 21
ScrFI CCNGG 1 cut(s) 12
SduI GDGCHC 2 cut(s) 73, 192
SetI ASST 7 cut(s) 73, 114, 124, 149, 256, 265, 277
SfcI CTRYAG 1 cut(s) 276
SinI GGWCC 1 cut(s) 21
SmiMI CAYNNNNRTG 1 cut(s) 197
Sse9I AATT 1 cut(s) 126
SsiI CCGC 1 cut(s) 230
SspI AATATT 1 cut(s) 164
SstI GAGCTC 1 cut(s) 73
StyD4I CCNGG 1 cut(s) 10
TaaI ACNGT 1 cut(s) 40
TasI AATT 1 cut(s) 126
Tru1I TTAA 1 cut(s) 27
Tru9I TTAA 1 cut(s) 27
TscAI CASTG 1 cut(s) 81
TseI GCWGC 2 cut(s) 268, 275
TspDTI ATGAA 3 cut(s) 17, 215, 236
TspRI CASTG 1 cut(s) 81
VpaK11BI GGWCC 1 cut(s) 21
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.