Rmu_sc0003550.1_g000004

ER membrane protein complex subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003550.1
OG0015492
Physical Location & Seq
Forward (+)
22820 .. 26023
3204 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0003550.1_g000004.1.cds

Sequence Viewer

Length: 930 bp
atggcgcgctctctctccgccggcgacttgttgttcctcttcgcagtctccgccttgttgtgtgtttcagtaggtttccaatccgatgagctgttgctcgacgacgaggagttcggtctggaaggaggggtgcccaccaagtctcccgatctcacttacacgcgatcttcttcaccaccggcgcagccgcaaccgaccacaacaaccagccggaggaggttttccgatcaagataccgactccaagatccagttcgagctccatcacgccttcggggactctgatttctctcccgccggcaccttcagtgctcgcttaaagacatggagccatggtggtcagacgctaacgaagcttcggttttcgaggaatgcttttactgaagaggagaaggagaagttttctctactgctgaaaggagatgacttctatagcataaggttgccatccagtgttgcgaatcctcctgggagggattatgttatttcatcagtaaaagcgagatgtcttccgcgagagggtttggatgaacattttgttatacacacggatggagttaatattctggcagttaattatggttcccctggggcatgcccatatcctcggcaactgagacttccttcaaaatggtcttttaactctcacacagttttgaagaacagtgaacaggctccaagagctccagtgttttctgaggaggtttttggtggtgagggagtagaaggtgaagttgtaccgccacaagaaaggtctttctgggctaaatattggatgtacctgatccctcttgggctcattgttatgaatgccgtaacccaagcaatgaacatggccgaggaacaggttggtgctccctcagctgctggccaagcacagcagccagctgcaatccaacgtgggtcaagttcttctgtgcgaagaagatag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

309

Amino Acids

33.97

Weight (kDa)

5.92

Isoelectric Point (pI)

62.18

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015492)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G65270 AT1G65270 AT1G65270
fragaria_vesca FvH4_6g00240
malus_domestica MD04G1246900.v1.1 MD12G1263300.v1.1
prunus_persica Prupe.6G363100_v2.0.a1
pyrus_communis pycom04g21760 pycom12g24060
rosa_chinensis RchiOBHm_Chr3g0447611
rosa_laevigata RLG00000025984
rosa_multiflora Rmu_sc0003550.1_g000004
rosa_roxburghii Rroxscaffold_6G00425820
rosa_rugosa Rorug02G0603500
rosa_samantha Rh3AG003700
rosa_wichuraiana Rw3G000260

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 130, 299
AccII CGCG 3 cut(s) 7, 163, 514
AciI CCGC 6 cut(s) 18, 51, 188, 294, 512, 740
AclWI GGATC 2 cut(s) 241, 778
AcoI YGGCCR 2 cut(s) 834, 868
AcuI CTGAAG 2 cut(s) 289, 402
AfaI GTAC 2 cut(s) 738, 779
AfiI CCNNNNNNNGG 2 cut(s) 213, 518
AgsI TTSAA 2 cut(s) 627, 658
AjnI CCWGG 2 cut(s) 466, 586
AjuI GAANNNNNNNTTGG 2 cut(s) 236, 268
AluBI AGCT 6 cut(s) 91, 259, 355, 683, 863, 887
AluI AGCT 6 cut(s) 91, 259, 355, 683, 863, 887
Alw21I GWGCWC 4 cut(s) 261, 313, 685, 856
Alw26I GTCTC 3 cut(s) 52, 147, 610
AlwI GGATC 2 cut(s) 241, 778
AlwNI CAGNNNCTG 1 cut(s) 866
AoxI GGCC 2 cut(s) 834, 868
ApeKI GCWGC 4 cut(s) 184, 863, 880, 887
ArsI GACNNNNNNTTYG 2 cut(s) 95, 127
AspLEI GCGC 3 cut(s) 7, 9, 184
AsuHPI GGTGA 3 cut(s) 165, 725, 740
BaeGI GKGCMC 1 cut(s) 135
BalI TGGCCA 1 cut(s) 870
BanI GGYRCC 2 cut(s) 130, 299
BanII GRGCYC 3 cut(s) 261, 685, 798
BbsI GAAGAC 1 cut(s) 500
Bbv12I GWGCWC 4 cut(s) 261, 313, 685, 856
BbvCI CCTCAGC 1 cut(s) 859
BbvI GCAGC 4 cut(s) 196, 850, 874, 892
BccI CCATC 3 cut(s) 270, 454, 545
BceAI ACGGC 1 cut(s) 797
BciT130I CCWGG 2 cut(s) 468, 588
BcoDI GTCTC 3 cut(s) 52, 147, 610
BfmI CTRYAG 1 cut(s) 430
BisI GCNGC 5 cut(s) 185, 188, 864, 881, 888
BlsI GCNGC 5 cut(s) 186, 189, 865, 882, 889
Bme1390I CCNGG 2 cut(s) 468, 588
BmiI GGNNCC 5 cut(s) 132, 301, 329, 583, 675
BmrFI CCNGG 2 cut(s) 468, 588
BpiI GAAGAC 1 cut(s) 500
BpmI CTGGAG 1 cut(s) 669
Bpu10I CCTNAGC 1 cut(s) 859
BsaJI CCNNGG 6 cut(s) 331, 467, 586, 587, 605, 837
BsaXI ACNNNNNCTCC 4 cut(s) 127, 157, 319, 349
Bsc4I CCNNNNNNNGG 2 cut(s) 213, 518
Bse118I RCCGGY 3 cut(s) 20, 178, 296
Bse1I ACTGG 3 cut(s) 250, 450, 686
Bse3DI GCAATG 1 cut(s) 831
BseBI CCWGG 2 cut(s) 468, 588
BseDI CCNNGG 6 cut(s) 331, 467, 586, 587, 605, 837
BseGI GGATG 4 cut(s) 446, 532, 556, 780
BseLI CCNNNNNNNGG 2 cut(s) 213, 518
BseMI GCAATG 1 cut(s) 831
BseMII CTCAG 3 cut(s) 605, 687, 873
BseNI ACTGG 3 cut(s) 250, 450, 686
BsePI GCGCGC 1 cut(s) 5
BseRI GAGGAG 4 cut(s) 122, 229, 401, 713
BseSI GKGCMC 1 cut(s) 135
BseXI GCAGC 4 cut(s) 196, 850, 874, 892
Bsh1236I CGCG 3 cut(s) 7, 163, 514
BshFI GGCC 2 cut(s) 836, 870
BshNI GGYRCC 2 cut(s) 130, 299
BsiHKAI GWGCWC 4 cut(s) 261, 313, 685, 856
BsiSI CCGG 4 cut(s) 21, 179, 211, 297
BslFI GGGAC 1 cut(s) 290
BslI CCNNNNNNNGG 2 cut(s) 213, 518
BsmAI GTCTC 3 cut(s) 52, 147, 610
BsmFI GGGAC 1 cut(s) 290
BsmI GAATGC 2 cut(s) 376, 814
BsnI GGCC 2 cut(s) 836, 870
Bsp1286I GDGCHC 6 cut(s) 135, 261, 313, 685, 798, 856
Bsp143I GATC 5 cut(s) 148, 164, 226, 246, 783
Bsp19I CCATGG 1 cut(s) 331
BspACI CCGC 6 cut(s) 18, 51, 188, 294, 512, 740
BspANI GGCC 2 cut(s) 836, 870
BspCNI CTCAG 3 cut(s) 606, 688, 872
BspFNI CGCG 3 cut(s) 7, 163, 514
BspLI GGNNCC 5 cut(s) 132, 301, 329, 583, 675
BspPI GGATC 2 cut(s) 241, 778
BspT107I GGYRCC 2 cut(s) 130, 299
BsrDI GCAATG 1 cut(s) 831
BsrFI RCCGGY 3 cut(s) 20, 178, 296
BsrI ACTGG 3 cut(s) 250, 450, 686
BssAI RCCGGY 3 cut(s) 20, 178, 296
BssECI CCNNGG 6 cut(s) 331, 467, 586, 587, 605, 837
BssHII GCGCGC 1 cut(s) 5
BssMI GATC 5 cut(s) 148, 164, 226, 246, 783
BssT1I CCWWGG 1 cut(s) 331
Bst2UI CCWGG 2 cut(s) 468, 588
Bst4CI ACNGT 2 cut(s) 652, 665
Bst6I CTCTTC 2 cut(s) 44, 378
BstC8I GCNNGC 7 cut(s) 7, 22, 298, 313, 595, 868, 885
BstDEI CTNAG 3 cut(s) 614, 696, 859
BstDSI CCRYGG 1 cut(s) 331
BstF5I GGATG 4 cut(s) 446, 532, 556, 780
BstFNI CGCG 3 cut(s) 7, 163, 514
BstHHI GCGC 3 cut(s) 7, 9, 184
BstKTI GATC 5 cut(s) 151, 167, 229, 249, 786
BstMAI GTCTC 3 cut(s) 52, 147, 610
BstMBI GATC 5 cut(s) 148, 164, 226, 246, 783
BstMWI GCNNNNNNNGC 5 cut(s) 50, 352, 680, 860, 872
BstNI CCWGG 2 cut(s) 468, 588
BstNSI RCATGY 1 cut(s) 597
BstSCI CCNGG 2 cut(s) 466, 586
BstSFI CTRYAG 1 cut(s) 430
BstSLI GKGCMC 1 cut(s) 135
BstUI CGCG 3 cut(s) 7, 163, 514
BstV1I GCAGC 4 cut(s) 196, 850, 874, 892
BstV2I GAAGAC 1 cut(s) 500
BstX2I RGATCY 1 cut(s) 246
BstYI RGATCY 1 cut(s) 246
BsuRI GGCC 2 cut(s) 836, 870
BtgI CCRYGG 1 cut(s) 331
BtsCI GGATG 4 cut(s) 446, 532, 556, 780
BtsIMutI CAGTG 4 cut(s) 313, 457, 670, 693
Cac8I GCNNGC 7 cut(s) 7, 22, 298, 313, 595, 868, 885
CaiI CAGNNNCTG 1 cut(s) 866
CfoI GCGC 3 cut(s) 7, 9, 184
Cfr10I RCCGGY 3 cut(s) 20, 178, 296
CseI GACGC 1 cut(s) 352
Csp6I GTAC 2 cut(s) 737, 778
CviAII CATG 4 cut(s) 324, 332, 594, 832
CviQI GTAC 2 cut(s) 737, 778
DdeI CTNAG 3 cut(s) 614, 696, 859
DpnI GATC 5 cut(s) 150, 166, 228, 248, 785
DpnII GATC 5 cut(s) 148, 164, 226, 246, 783
EaeI YGGCCR 2 cut(s) 834, 868
Eam1104I CTCTTC 2 cut(s) 44, 378
EarI CTCTTC 2 cut(s) 44, 378
EciI GGCGGA 2 cut(s) 7, 40
Ecl136II GAGCTC 2 cut(s) 259, 683
Eco130I CCWWGG 1 cut(s) 331
Eco24I GRGCYC 3 cut(s) 261, 685, 798
Eco53kI GAGCTC 2 cut(s) 259, 683
Eco57I CTGAAG 2 cut(s) 289, 402
EcoICRI GAGCTC 2 cut(s) 259, 683
EcoRII CCWGG 2 cut(s) 466, 586
EcoT14I CCWWGG 1 cut(s) 331
EcoT38I GRGCYC 3 cut(s) 261, 685, 798
ErhI CCWWGG 1 cut(s) 331
FaeI CATG 4 cut(s) 327, 335, 597, 835
FaqI GGGAC 1 cut(s) 290
FatI CATG 4 cut(s) 323, 331, 593, 831
FauI CCCGC 1 cut(s) 301
Fnu4HI GCNGC 5 cut(s) 185, 188, 864, 881, 888
FokI GGATG 4 cut(s) 433, 539, 563, 787
FriOI GRGCYC 3 cut(s) 261, 685, 798
Fsp4HI GCNGC 5 cut(s) 185, 188, 864, 881, 888
GlaI GCGC 3 cut(s) 6, 8, 183
GluI GCNGC 5 cut(s) 185, 188, 864, 881, 888
GsuI CTGGAG 1 cut(s) 669
HaeIII GGCC 2 cut(s) 836, 870
HapII CCGG 4 cut(s) 21, 179, 211, 297
HgaI GACGC 1 cut(s) 352
HhaI GCGC 3 cut(s) 7, 9, 184
Hin1II CATG 4 cut(s) 327, 335, 597, 835
Hin6I GCGC 3 cut(s) 5, 7, 182
HinP1I GCGC 3 cut(s) 5, 7, 182
HindIII AAGCTT 1 cut(s) 353
HinfI GANTC 3 cut(s) 239, 278, 460
HpaII CCGG 4 cut(s) 21, 179, 211, 297
HphI GGTGA 3 cut(s) 165, 725, 740
Hpy166II GTNNAC 1 cut(s) 668
Hpy188I TCNGA 5 cut(s) 85, 226, 283, 342, 697
Hpy188III TCNNGA 3 cut(s) 119, 146, 230
Hpy8I GTNNAC 1 cut(s) 668
Hpy99I CGWCG 2 cut(s) 104, 107
HpyAV CCTTC 6 cut(s) 116, 280, 313, 385, 633, 719
HpyCH4III ACNGT 2 cut(s) 652, 665
HpyCH4IV ACGT 1 cut(s) 898
HpyCH4V TGCA 1 cut(s) 890
HpyF10VI GCNNNNNNNGC 5 cut(s) 50, 352, 680, 860, 872
HpyF3I CTNAG 3 cut(s) 614, 696, 859
HpySE526I ACGT 1 cut(s) 898
Hsp92II CATG 4 cut(s) 327, 335, 597, 835
HspAI GCGC 3 cut(s) 5, 7, 182
KroI GCCGGC 2 cut(s) 20, 296
KroNI GCCGGC 2 cut(s) 22, 298
Kzo9I GATC 5 cut(s) 148, 164, 226, 246, 783
LmnI GCTCC 5 cut(s) 264, 327, 679, 688, 859
Lsp1109I GCAGC 4 cut(s) 196, 850, 874, 892
MaeII ACGT 1 cut(s) 898
MaeIII GTNAC 1 cut(s) 814
MalI GATC 5 cut(s) 150, 166, 228, 248, 785
MboI GATC 5 cut(s) 148, 164, 226, 246, 783
MboII GAAGA 7 cut(s) 31, 159, 162, 395, 500, 670, 903
MflI RGATCY 1 cut(s) 246
MhlI GDGCHC 6 cut(s) 135, 261, 313, 685, 798, 856
MlsI TGGCCA 1 cut(s) 870
MluCI AATT 1 cut(s) 574
MluNI TGGCCA 1 cut(s) 870
MlyI GAGTC 2 cut(s) 233, 272
MmeI TCCRAC 1 cut(s) 919
Mox20I TGGCCA 1 cut(s) 870
MreI CGCCGGCG 1 cut(s) 20
MroNI GCCGGC 2 cut(s) 20, 296
MscI TGGCCA 1 cut(s) 870
MseI TTAA 4 cut(s) 317, 558, 573, 639
MslI CAYNNNNRTG 2 cut(s) 549, 803
Msp20I TGGCCA 1 cut(s) 870
MspA1I CMGCKG 2 cut(s) 863, 887
MspI CCGG 4 cut(s) 21, 179, 211, 297
MspR9I CCNGG 2 cut(s) 468, 588
Mva1269I GAATGC 2 cut(s) 376, 814
MvaI CCWGG 2 cut(s) 468, 588
MvnI CGCG 3 cut(s) 7, 163, 514
MwoI GCNNNNNNNGC 5 cut(s) 50, 352, 680, 860, 872
NaeI GCCGGC 2 cut(s) 22, 298
NcoI CCATGG 1 cut(s) 331
NdeII GATC 5 cut(s) 148, 164, 226, 246, 783
NgoMIV GCCGGC 2 cut(s) 20, 296
NlaIII CATG 4 cut(s) 327, 335, 597, 835
NlaIV GGNNCC 5 cut(s) 132, 301, 329, 583, 675
NmeAIII GCCGAG 2 cut(s) 586, 862
NspI RCATGY 1 cut(s) 597
PaeI GCATGC 1 cut(s) 597
PasI CCCWGGG 1 cut(s) 587
PauI GCGCGC 1 cut(s) 5
PctI GAATGC 2 cut(s) 376, 814
PdiI GCCGGC 2 cut(s) 22, 298
PfeI GAWTC 1 cut(s) 460
PkrI GCNGC 5 cut(s) 186, 189, 865, 882, 889
PleI GAGTC 2 cut(s) 233, 272
PpsI GAGTC 2 cut(s) 233, 272
Psp124BI GAGCTC 2 cut(s) 261, 685
Psp6I CCWGG 2 cut(s) 466, 586
PspGI CCWGG 2 cut(s) 466, 586
PspN4I GGNNCC 5 cut(s) 132, 301, 329, 583, 675
PstNI CAGNNNCTG 1 cut(s) 866
PsuI RGATCY 1 cut(s) 246
PteI GCGCGC 1 cut(s) 5
PvuII CAGCTG 2 cut(s) 863, 887
RsaI GTAC 2 cut(s) 738, 779
RsaNI GTAC 2 cut(s) 737, 778
RseI CAYNNNNRTG 2 cut(s) 549, 803
SacI GAGCTC 2 cut(s) 261, 685
SaqAI TTAA 4 cut(s) 317, 558, 573, 639
SatI GCNGC 5 cut(s) 185, 188, 864, 881, 888
Sau3AI GATC 5 cut(s) 148, 164, 226, 246, 783
SchI GAGTC 2 cut(s) 233, 272
ScrFI CCNGG 2 cut(s) 468, 588
SduI GDGCHC 6 cut(s) 135, 261, 313, 685, 798, 856
SfcI CTRYAG 1 cut(s) 430
SgrAI CRCCGGYG 2 cut(s) 20, 178
SmiMI CAYNNNNRTG 2 cut(s) 549, 803
SphI GCATGC 1 cut(s) 597
Sse9I AATT 1 cut(s) 574
SsiI CCGC 6 cut(s) 18, 51, 188, 294, 512, 740
SspI AATATT 2 cut(s) 562, 770
SstI GAGCTC 2 cut(s) 261, 685
StyD4I CCNGG 2 cut(s) 466, 586
StyI CCWWGG 1 cut(s) 331
TaaI ACNGT 2 cut(s) 652, 665
TaiI ACGT 1 cut(s) 901
TaqI TCGA 3 cut(s) 99, 255, 365
TaqII GACCGA 1 cut(s) 104
TasI AATT 1 cut(s) 574
TauI GCSGC 1 cut(s) 190
TfiI GAWTC 1 cut(s) 460
Tru1I TTAA 4 cut(s) 317, 558, 573, 639
Tru9I TTAA 4 cut(s) 317, 558, 573, 639
TscAI CASTG 4 cut(s) 313, 457, 670, 693
TseI GCWGC 4 cut(s) 184, 863, 880, 887
TspDTI ATGAA 4 cut(s) 477, 543, 821, 842
TspGWI ACGGA 1 cut(s) 563
TspRI CASTG 4 cut(s) 313, 457, 670, 693
XceI RCATGY 1 cut(s) 597
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.