Rroxscaffold_6G00425820

ER membrane protein complex subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0015492
Physical Location & Seq
Reverse (-)
46795808 .. 46799365
3558 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00425820.1

Sequence Viewer

Length: 936 bp
ATGGCGCGCTCTCTCTCCGCCGGCGACTTGTTGTTCCTCTTCGCAGTCTCCGCCTTGTTGTACGTTTCAGTAGCTTTTCAATCGGATGAGCTGTTGCTGGACGACGAAGAGTTCGGTCTGGAAGGAGGGGTGCACACCAAGTCTCCCGATCTCACTTACACGCGATCTTCGTCACCACCGGCGCAGCCGCAGCCGCCGACCACAACAACTAGCCGTAGGAGATTTTCCGATCAAGATACCGACTCCAAGATCCAGTTCGAGCTCCATCACGCCTTCGGGGACTCTGATTTCTCTCCAGCCGGCACCTTCAGCGCTCGCTTAAAGACATGGAGCCACGGTGGTCAGACGCTAACGAAGCTTCGGTTTTCGAGGAATGCTTTTACTGAAGAGGAGAGGGAGAAGTTTTCTCTACTGCTGAAAGGAGATGCCTTCTATAGCATAAGGTTGCCATCCAGTGTTGCAAATCCTCCTGGGAGGGATTATGTTATTTCATCAGTAAAAGCGGCAAGAATAGTTCTAAGAATTGGAAGAGACTTGGTTAGATTTGTTTTGCAGGATGGAGTTAATATTCTGGCAGTTAATTATGGTTCCCCTGGGGCATGCCCATATCCTCGGCAACTGAGACTTCCTTCAAAATGGTCTTTTAACTCTCACACAGTTTTGAAGAACAGTGAACAGGCACCAAGAGCTCCAGTGTTTTCTGAGGAGATTTTTGGTGGTGAGGGAGTAGAAGGTGAAGTTGTACTGCCACAAGAAAGGTCTTTCTGGGCTAAATATTGGATGTACCTGATCCCTCTTGGGCTCATTGTTATGAATGCCGTAACCCAAGCAATGAACATGGCCGAGGAACAGGTTGGTGCTCCCTCAGCTGCTGGCCAAGCACAGCAGCCAGCTGCAATCCAACGTGGGTCAAGTTCTTCTGTGCGAAGAAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

311

Amino Acids

34.29

Weight (kDa)

7.86

Isoelectric Point (pI)

63.8

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015492)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G65270 AT1G65270 AT1G65270
fragaria_vesca FvH4_6g00240
malus_domestica MD04G1246900.v1.1 MD12G1263300.v1.1
prunus_persica Prupe.6G363100_v2.0.a1
pyrus_communis pycom04g21760 pycom12g24060
rosa_chinensis RchiOBHm_Chr3g0447611
rosa_laevigata RLG00000025984
rosa_multiflora Rmu_sc0003550.1_g000004
rosa_roxburghii Rroxscaffold_6G00425820
rosa_rugosa Rorug02G0603500
rosa_samantha Rh3AG003700
rosa_wichuraiana Rw3G000260

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 302, 679
AccII CGCG 2 cut(s) 7, 163
AciI CCGC 5 cut(s) 18, 51, 188, 194, 503
AclWI GGATC 2 cut(s) 244, 784
AcoI YGGCCR 2 cut(s) 840, 874
AcuI CTGAAG 2 cut(s) 292, 405
AfaI GTAC 3 cut(s) 62, 744, 785
AfeI AGCGCT 1 cut(s) 313
AgsI TTSAA 3 cut(s) 80, 633, 664
AjnI CCWGG 2 cut(s) 469, 592
AjuI GAANNNNNNNTTGG 2 cut(s) 239, 271
AluBI AGCT 7 cut(s) 74, 91, 262, 358, 689, 869, 893
AluI AGCT 7 cut(s) 74, 91, 262, 358, 689, 869, 893
Alw21I GWGCWC 4 cut(s) 135, 264, 691, 862
Alw26I GTCTC 4 cut(s) 52, 147, 525, 616
Alw44I GTGCAC 1 cut(s) 131
AlwI GGATC 2 cut(s) 244, 784
AlwNI CAGNNNCTG 1 cut(s) 872
Aor51HI AGCGCT 1 cut(s) 313
AoxI GGCC 2 cut(s) 840, 874
ApaLI GTGCAC 1 cut(s) 131
ApeKI GCWGC 5 cut(s) 184, 190, 869, 886, 893
ArsI GACNNNNNNTTYG 2 cut(s) 95, 127
AspLEI GCGC 4 cut(s) 7, 9, 184, 314
AsuHPI GGTGA 3 cut(s) 165, 731, 746
BaeGI GKGCMC 1 cut(s) 135
BalI TGGCCA 1 cut(s) 876
BanI GGYRCC 2 cut(s) 302, 679
BanII GRGCYC 3 cut(s) 264, 691, 804
Bbv12I GWGCWC 4 cut(s) 135, 264, 691, 862
BbvCI CCTCAGC 1 cut(s) 865
BbvI GCAGC 5 cut(s) 196, 202, 856, 880, 898
BccI CCATC 3 cut(s) 273, 457, 551
BceAI ACGGC 2 cut(s) 198, 803
BciT130I CCWGG 2 cut(s) 471, 594
BcoDI GTCTC 4 cut(s) 52, 147, 525, 616
BfaI CTAG 1 cut(s) 210
BfmI CTRYAG 1 cut(s) 433
BfoI RGCGCY 1 cut(s) 315
BisI GCNGC 8 cut(s) 185, 188, 191, 194, 504, 870, 887, 894
BlsI GCNGC 8 cut(s) 186, 189, 192, 195, 505, 871, 888, 895
Bme1390I CCNGG 2 cut(s) 471, 594
BmiI GGNNCC 4 cut(s) 304, 332, 589, 681
BmrFI CCNGG 2 cut(s) 471, 594
BmsI GCATC 1 cut(s) 415
BpmI CTGGAG 2 cut(s) 279, 675
Bpu10I CCTNAGC 1 cut(s) 865
BsaJI CCNNGG 6 cut(s) 334, 470, 592, 593, 611, 843
BsaXI ACNNNNNCTCC 4 cut(s) 127, 157, 322, 352
Bse118I RCCGGY 3 cut(s) 20, 178, 299
Bse1I ACTGG 3 cut(s) 253, 453, 692
Bse3DI GCAATG 1 cut(s) 837
BseBI CCWGG 2 cut(s) 471, 594
BseDI CCNNGG 6 cut(s) 334, 470, 592, 593, 611, 843
BseGI GGATG 4 cut(s) 91, 449, 562, 786
BseMI GCAATG 1 cut(s) 837
BseMII CTCAG 3 cut(s) 611, 693, 879
BseNI ACTGG 3 cut(s) 253, 453, 692
BsePI GCGCGC 1 cut(s) 5
BseRI GAGGAG 2 cut(s) 404, 719
BseSI GKGCMC 1 cut(s) 135
BseXI GCAGC 5 cut(s) 196, 202, 856, 880, 898
Bsh1236I CGCG 2 cut(s) 7, 163
BshFI GGCC 2 cut(s) 842, 876
BshNI GGYRCC 2 cut(s) 302, 679
BsiHKAI GWGCWC 4 cut(s) 135, 264, 691, 862
BsiSI CCGG 3 cut(s) 21, 179, 300
BslFI GGGAC 1 cut(s) 293
BsmAI GTCTC 4 cut(s) 52, 147, 525, 616
BsmFI GGGAC 1 cut(s) 293
BsmI GAATGC 2 cut(s) 379, 820
BsnI GGCC 2 cut(s) 842, 876
Bsp1286I GDGCHC 5 cut(s) 135, 264, 691, 804, 862
Bsp143I GATC 5 cut(s) 148, 164, 229, 249, 789
BspACI CCGC 5 cut(s) 18, 51, 188, 194, 503
BspANI GGCC 2 cut(s) 842, 876
BspCNI CTCAG 3 cut(s) 612, 694, 878
BspFNI CGCG 2 cut(s) 7, 163
BspLI GGNNCC 4 cut(s) 304, 332, 589, 681
BspPI GGATC 2 cut(s) 244, 784
BspT107I GGYRCC 2 cut(s) 302, 679
BsrDI GCAATG 1 cut(s) 837
BsrFI RCCGGY 3 cut(s) 20, 178, 299
BsrI ACTGG 3 cut(s) 253, 453, 692
BssAI RCCGGY 3 cut(s) 20, 178, 299
BssECI CCNNGG 6 cut(s) 334, 470, 592, 593, 611, 843
BssHII GCGCGC 1 cut(s) 5
BssMI GATC 5 cut(s) 148, 164, 229, 249, 789
Bst2UI CCWGG 2 cut(s) 471, 594
Bst4CI ACNGT 3 cut(s) 338, 658, 671
Bst6I CTCTTC 4 cut(s) 44, 102, 381, 523
BstC8I GCNNGC 7 cut(s) 7, 22, 301, 316, 601, 874, 891
BstDEI CTNAG 4 cut(s) 518, 620, 702, 865
BstDSI CCRYGG 1 cut(s) 334
BstF5I GGATG 4 cut(s) 91, 449, 562, 786
BstFNI CGCG 2 cut(s) 7, 163
BstH2I RGCGCY 1 cut(s) 315
BstHHI GCGC 4 cut(s) 7, 9, 184, 314
BstKTI GATC 5 cut(s) 151, 167, 232, 252, 792
BstMAI GTCTC 4 cut(s) 52, 147, 525, 616
BstMBI GATC 5 cut(s) 148, 164, 229, 249, 789
BstMWI GCNNNNNNNGC 8 cut(s) 50, 190, 193, 309, 355, 686, 866, 878
BstNI CCWGG 2 cut(s) 471, 594
BstNSI RCATGY 1 cut(s) 603
BstSCI CCNGG 2 cut(s) 469, 592
BstSFI CTRYAG 1 cut(s) 433
BstSLI GKGCMC 1 cut(s) 135
BstUI CGCG 2 cut(s) 7, 163
BstV1I GCAGC 5 cut(s) 196, 202, 856, 880, 898
BstX2I RGATCY 1 cut(s) 249
BstYI RGATCY 1 cut(s) 249
BsuRI GGCC 2 cut(s) 842, 876
BtgI CCRYGG 1 cut(s) 334
BtsCI GGATG 4 cut(s) 91, 449, 562, 786
BtsIMutI CAGTG 3 cut(s) 460, 676, 699
Cac8I GCNNGC 7 cut(s) 7, 22, 301, 316, 601, 874, 891
CaiI CAGNNNCTG 1 cut(s) 872
CfoI GCGC 4 cut(s) 7, 9, 184, 314
Cfr10I RCCGGY 3 cut(s) 20, 178, 299
CseI GACGC 1 cut(s) 355
Csp6I GTAC 3 cut(s) 61, 743, 784
CviAII CATG 3 cut(s) 327, 600, 838
CviQI GTAC 3 cut(s) 61, 743, 784
DdeI CTNAG 4 cut(s) 518, 620, 702, 865
DpnI GATC 5 cut(s) 150, 166, 231, 251, 791
DpnII GATC 5 cut(s) 148, 164, 229, 249, 789
EaeI YGGCCR 2 cut(s) 840, 874
Eam1104I CTCTTC 4 cut(s) 44, 102, 381, 523
EarI CTCTTC 4 cut(s) 44, 102, 381, 523
EciI GGCGGA 2 cut(s) 7, 40
Ecl136II GAGCTC 2 cut(s) 262, 689
Eco24I GRGCYC 3 cut(s) 264, 691, 804
Eco47III AGCGCT 1 cut(s) 313
Eco53kI GAGCTC 2 cut(s) 262, 689
Eco57I CTGAAG 2 cut(s) 292, 405
EcoICRI GAGCTC 2 cut(s) 262, 689
EcoRII CCWGG 2 cut(s) 469, 592
EcoT38I GRGCYC 3 cut(s) 264, 691, 804
FaeI CATG 3 cut(s) 330, 603, 841
FaiI YATR 9 cut(s) 328, 435, 440, 483, 585, 601, 607, 812, 839
FaqI GGGAC 1 cut(s) 293
FatI CATG 3 cut(s) 326, 599, 837
Fnu4HI GCNGC 8 cut(s) 185, 188, 191, 194, 504, 870, 887, 894
FokI GGATG 4 cut(s) 98, 436, 569, 793
FriOI GRGCYC 3 cut(s) 264, 691, 804
Fsp4HI GCNGC 8 cut(s) 185, 188, 191, 194, 504, 870, 887, 894
FspBI CTAG 1 cut(s) 210
GlaI GCGC 4 cut(s) 6, 8, 183, 313
GluI GCNGC 8 cut(s) 185, 188, 191, 194, 504, 870, 887, 894
GsuI CTGGAG 2 cut(s) 279, 675
HaeII RGCGCY 1 cut(s) 315
HaeIII GGCC 2 cut(s) 842, 876
HapII CCGG 3 cut(s) 21, 179, 300
HgaI GACGC 1 cut(s) 355
HhaI GCGC 4 cut(s) 7, 9, 184, 314
Hin1II CATG 3 cut(s) 330, 603, 841
Hin6I GCGC 4 cut(s) 5, 7, 182, 312
HinP1I GCGC 4 cut(s) 5, 7, 182, 312
HindIII AAGCTT 1 cut(s) 356
HinfI GANTC 2 cut(s) 242, 281
HpaII CCGG 3 cut(s) 21, 179, 300
HphI GGTGA 3 cut(s) 165, 731, 746
Hpy166II GTNNAC 2 cut(s) 133, 674
Hpy188I TCNGA 5 cut(s) 85, 229, 286, 345, 703
Hpy188III TCNNGA 3 cut(s) 119, 146, 233
Hpy8I GTNNAC 2 cut(s) 133, 674
Hpy99I CGWCG 1 cut(s) 107
HpyAV CCTTC 6 cut(s) 116, 283, 316, 439, 639, 725
HpyCH4III ACNGT 3 cut(s) 338, 658, 671
HpyCH4IV ACGT 2 cut(s) 63, 904
HpyCH4V TGCA 4 cut(s) 133, 461, 553, 896
HpyF10VI GCNNNNNNNGC 8 cut(s) 50, 190, 193, 309, 355, 686, 866, 878
HpyF3I CTNAG 4 cut(s) 518, 620, 702, 865
HpySE526I ACGT 2 cut(s) 63, 904
Hsp92II CATG 3 cut(s) 330, 603, 841
HspAI GCGC 4 cut(s) 5, 7, 182, 312
KroI GCCGGC 2 cut(s) 20, 299
KroNI GCCGGC 2 cut(s) 22, 301
Kzo9I GATC 5 cut(s) 148, 164, 229, 249, 789
LmnI GCTCC 4 cut(s) 267, 330, 694, 865
Lsp1109I GCAGC 5 cut(s) 196, 202, 856, 880, 898
LweI GCATC 1 cut(s) 415
MaeI CTAG 1 cut(s) 210
MaeII ACGT 2 cut(s) 63, 904
MaeIII GTNAC 2 cut(s) 171, 820
MalI GATC 5 cut(s) 150, 166, 231, 251, 791
MboI GATC 5 cut(s) 148, 164, 229, 249, 789
MboII GAAGA 7 cut(s) 31, 119, 159, 398, 540, 676, 909
MflI RGATCY 1 cut(s) 249
MhlI GDGCHC 5 cut(s) 135, 264, 691, 804, 862
MlsI TGGCCA 1 cut(s) 876
MluCI AATT 2 cut(s) 522, 580
MluNI TGGCCA 1 cut(s) 876
MlyI GAGTC 2 cut(s) 236, 275
MmeI TCCRAC 1 cut(s) 925
Mox20I TGGCCA 1 cut(s) 876
MreI CGCCGGCG 1 cut(s) 20
MroNI GCCGGC 2 cut(s) 20, 299
MscI TGGCCA 1 cut(s) 876
MseI TTAA 4 cut(s) 320, 564, 579, 645
MslI CAYNNNNRTG 1 cut(s) 809
Msp20I TGGCCA 1 cut(s) 876
MspA1I CMGCKG 2 cut(s) 869, 893
MspI CCGG 3 cut(s) 21, 179, 300
MspR9I CCNGG 2 cut(s) 471, 594
Mva1269I GAATGC 2 cut(s) 379, 820
MvaI CCWGG 2 cut(s) 471, 594
MvnI CGCG 2 cut(s) 7, 163
MwoI GCNNNNNNNGC 8 cut(s) 50, 190, 193, 309, 355, 686, 866, 878
NaeI GCCGGC 2 cut(s) 22, 301
NdeII GATC 5 cut(s) 148, 164, 229, 249, 789
NgoMIV GCCGGC 2 cut(s) 20, 299
NlaIII CATG 3 cut(s) 330, 603, 841
NlaIV GGNNCC 4 cut(s) 304, 332, 589, 681
NmeAIII GCCGAG 2 cut(s) 592, 868
NmuCI GTSAC 1 cut(s) 171
NspI RCATGY 1 cut(s) 603
PaeI GCATGC 1 cut(s) 603
PasI CCCWGGG 1 cut(s) 593
PauI GCGCGC 1 cut(s) 5
PcsI WCGNNNNNNNCGW 1 cut(s) 167
PctI GAATGC 2 cut(s) 379, 820
PdiI GCCGGC 2 cut(s) 22, 301
PkrI GCNGC 8 cut(s) 186, 189, 192, 195, 505, 871, 888, 895
PleI GAGTC 2 cut(s) 236, 275
PpsI GAGTC 2 cut(s) 236, 275
Psp124BI GAGCTC 2 cut(s) 264, 691
Psp6I CCWGG 2 cut(s) 469, 592
PspGI CCWGG 2 cut(s) 469, 592
PspN4I GGNNCC 4 cut(s) 304, 332, 589, 681
PstNI CAGNNNCTG 1 cut(s) 872
PsuI RGATCY 1 cut(s) 249
PteI GCGCGC 1 cut(s) 5
PvuII CAGCTG 2 cut(s) 869, 893
RsaI GTAC 3 cut(s) 62, 744, 785
RsaNI GTAC 3 cut(s) 61, 743, 784
RseI CAYNNNNRTG 1 cut(s) 809
SacI GAGCTC 2 cut(s) 264, 691
SaqAI TTAA 4 cut(s) 320, 564, 579, 645
SatI GCNGC 8 cut(s) 185, 188, 191, 194, 504, 870, 887, 894
Sau3AI GATC 5 cut(s) 148, 164, 229, 249, 789
SchI GAGTC 2 cut(s) 236, 275
ScrFI CCNGG 2 cut(s) 471, 594
SduI GDGCHC 5 cut(s) 135, 264, 691, 804, 862
SfaNI GCATC 1 cut(s) 415
SfcI CTRYAG 1 cut(s) 433
SgrAI CRCCGGYG 2 cut(s) 20, 178
SmiMI CAYNNNNRTG 1 cut(s) 809
SphI GCATGC 1 cut(s) 603
Sse9I AATT 2 cut(s) 522, 580
SsiI CCGC 5 cut(s) 18, 51, 188, 194, 503
SspI AATATT 2 cut(s) 568, 776
SspMI CTAG 1 cut(s) 210
SstI GAGCTC 2 cut(s) 264, 691
StyD4I CCNGG 2 cut(s) 469, 592
TaaI ACNGT 3 cut(s) 338, 658, 671
TaiI ACGT 2 cut(s) 66, 907
TaqI TCGA 2 cut(s) 258, 368
TaqII GACCGA 1 cut(s) 104
TasI AATT 2 cut(s) 522, 580
TatI WGTACW 1 cut(s) 742
TauI GCSGC 3 cut(s) 190, 196, 506
Tru1I TTAA 4 cut(s) 320, 564, 579, 645
Tru9I TTAA 4 cut(s) 320, 564, 579, 645
TscAI CASTG 3 cut(s) 460, 676, 699
TseFI GTSAC 1 cut(s) 171
TseI GCWGC 5 cut(s) 184, 190, 869, 886, 893
Tsp45I GTSAC 1 cut(s) 171
TspDTI ATGAA 3 cut(s) 480, 827, 848
TspRI CASTG 3 cut(s) 460, 676, 699
VneI GTGCAC 1 cut(s) 131
XceI RCATGY 1 cut(s) 603
XspI CTAG 1 cut(s) 210
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.