MD04G1246900.v1.1

ER membrane protein complex subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0015492
Physical Location & Seq
Forward (+)
32181374 .. 32184331
2958 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1246900.v1.1.491

Sequence Viewer

Length: 939 bp
ATGGCGCTCTCTCTCTCCTCCTCTCGGTCGTCCCAGTGGCGGTTGATCTTCGTAATCTCCGCCGTGCTGTCTACTACAGTAGCAGCAGCTTTCCAATCCGATGAGCTCTTGATGGACGACGAGGAGTTCGGTCTAGAAGGAGGGCTGCACACTAAGTCTCCTGATCTCACATACACCAAATCCACGCCTACGCCAGCATCACCAACTACGTCGCCGACCCGGAAGAGGTTTTCGGATCCGGATTCGGACTCAAAGATCCAGTTTCAGCTTCATCACGCCTTTGGAGACTCTGATTTCTCGCCGGCTGGTACCTTCAGTGCTCGCTTAAAGACGTGGAATCACGGTGGTCAGACTCTCACAAAGCTACGATTTTCGAGGAATGCCTTTACCGAAGAGGAGAAGGAGAAATTTGCACTGCTACTGAAGGGAGATGACTTCTATAGGATAAGATTGCCATCCAGCGTCTTGAATCCTCCTGGGAGGGATTATGTCATTTCGTCAGTAAAAGCGAGATGTCTTCCACGAGAGGGTTTGGATGAGCATTTTGTTATACACACGGATGAAATTAACATCTTGGCAGTTAATTATGGTTCTCCTGGGGCATGTCCATATCCTCGGCAAATGAAACTTCCTGGAAAATGGTCCTTTAACTCTCACACGGTTTTGAAGAATAGTGAGCAGGCACCAAGAGCTCCAGTGTTCGCTGAGGAGATTCTGCGGGTTGGGGGTGAGGTAGGAGAAGGTGAAATTGTACCACCACCAGAAAGGTCGTTTTGGGCTAAATATTGGATGTACCTGATCCCTCTTGGGCTCATTGTCATGAATGCGATTACTCAAGCAATGAACATGGCGGAGGAGGGTGCTGGTCATCCAGCAGGCCAAGCTCAGCAGCCGGCTGCAGTGCAGCGTGGGTCAAGTTCCGCTGTGCGGAGAAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

313

Amino Acids

34.47

Weight (kDa)

7.11

Isoelectric Point (pI)

55.98

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015492)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G65270 AT1G65270 AT1G65270
fragaria_vesca FvH4_6g00240
malus_domestica MD04G1246900.v1.1 MD12G1263300.v1.1
prunus_persica Prupe.6G363100_v2.0.a1
pyrus_communis pycom04g21760 pycom12g24060
rosa_chinensis RchiOBHm_Chr3g0447611
rosa_laevigata RLG00000025984
rosa_multiflora Rmu_sc0003550.1_g000004
rosa_roxburghii Rroxscaffold_6G00425820
rosa_rugosa Rorug02G0603500
rosa_samantha Rh3AG003700
rosa_wichuraiana Rw3G000260

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 308
AccB1I GGYRCC 2 cut(s) 308, 682
AccI GTMKAC 1 cut(s) 71
AccIII TCCGGA 1 cut(s) 238
AciI CCGC 6 cut(s) 40, 60, 718, 851, 921, 928
AclWI GGATC 4 cut(s) 230, 243, 250, 793
AcsI RAATTY 1 cut(s) 407
AcuI CTGAAG 2 cut(s) 298, 443
AfaI GTAC 3 cut(s) 310, 753, 794
AfiI CCNNNNNNNGG 5 cut(s) 24, 39, 225, 527, 927
AgsI TTSAA 2 cut(s) 469, 667
AjiI CACGTC 1 cut(s) 333
AjnI CCWGG 3 cut(s) 475, 595, 631
AluBI AGCT 6 cut(s) 89, 106, 268, 364, 692, 884
AluI AGCT 6 cut(s) 89, 106, 268, 364, 692, 884
Alw21I GWGCWC 3 cut(s) 108, 322, 694
Alw26I GTCTC 2 cut(s) 162, 279
AlwI GGATC 4 cut(s) 230, 243, 250, 793
Aor13HI TCCGGA 1 cut(s) 238
AoxI GGCC 1 cut(s) 877
ApeKI GCWGC 6 cut(s) 83, 86, 145, 889, 896, 904
ApoI RAATTY 1 cut(s) 407
ArsI GACNNNNNNTTYG 2 cut(s) 110, 142
Asp718I GGTACC 1 cut(s) 308
AspLEI GCGC 1 cut(s) 7
AspS9I GGNCC 1 cut(s) 642
AsuC2I CCSGG 1 cut(s) 220
AsuHPI GGTGA 3 cut(s) 192, 740, 755
AvaII GGWCC 1 cut(s) 642
BamHI GGATCC 1 cut(s) 235
BanI GGYRCC 2 cut(s) 308, 682
BanII GRGCYC 3 cut(s) 108, 694, 813
BauI CACGAG 1 cut(s) 522
BbsI GAAGAC 1 cut(s) 509
Bbv12I GWGCWC 3 cut(s) 108, 322, 694
BbvCI CCTCAGC 1 cut(s) 705
BbvI GCAGC 6 cut(s) 95, 98, 132, 883, 901, 916
BccI CCATC 2 cut(s) 106, 463
BceAI ACGGC 1 cut(s) 47
BciT130I CCWGG 3 cut(s) 477, 597, 633
BcnI CCSGG 1 cut(s) 220
BcoDI GTCTC 2 cut(s) 162, 279
BfaI CTAG 1 cut(s) 134
BfmI CTRYAG 3 cut(s) 75, 439, 897
BfoI RGCGCY 1 cut(s) 8
BisI GCNGC 6 cut(s) 84, 87, 146, 890, 897, 905
BlpI GCTNAGC 1 cut(s) 885
BlsI GCNGC 6 cut(s) 85, 88, 147, 891, 898, 906
Bme1390I CCNGG 4 cut(s) 220, 477, 597, 633
Bme18I GGWCC 1 cut(s) 642
BmgBI CACGTC 1 cut(s) 333
BmgT120I GGNCC 1 cut(s) 642
BmiI GGNNCC 3 cut(s) 237, 310, 684
BmrFI CCNGG 4 cut(s) 220, 477, 597, 633
BmrI ACTGGG 1 cut(s) 28
BmsI GCATC 1 cut(s) 206
BmuI ACTGGG 1 cut(s) 28
BpiI GAAGAC 1 cut(s) 509
BpmI CTGGAG 1 cut(s) 678
Bpu10I CCTNAGC 1 cut(s) 705
Bpu1102I GCTNAGC 1 cut(s) 885
BpuEI CTTGAG 1 cut(s) 819
BpuMI CCSGG 1 cut(s) 220
BsaBI GATNNNNATC 1 cut(s) 454
BsaJI CCNNGG 3 cut(s) 476, 596, 614
BsaWI WCCGGW 1 cut(s) 238
BsaXI ACNNNNNCTCC 2 cut(s) 142, 172
Bsc4I CCNNNNNNNGG 5 cut(s) 24, 39, 225, 527, 927
Bse118I RCCGGY 2 cut(s) 301, 892
Bse1I ACTGG 3 cut(s) 34, 259, 695
Bse3DI GCAATG 1 cut(s) 846
Bse8I GATNNNNATC 1 cut(s) 454
BseAI TCCGGA 1 cut(s) 238
BseBI CCWGG 3 cut(s) 477, 597, 633
BseDI CCNNGG 3 cut(s) 476, 596, 614
BseGI GGATG 5 cut(s) 455, 541, 565, 795, 868
BseJI GATNNNNATC 1 cut(s) 454
BseLI CCNNNNNNNGG 5 cut(s) 24, 39, 225, 527, 927
BseMI GCAATG 1 cut(s) 846
BseMII CTCAG 2 cut(s) 696, 899
BseNI ACTGG 3 cut(s) 34, 259, 695
BseRI GAGGAG 6 cut(s) 7, 10, 137, 410, 722, 869
BseXI GCAGC 6 cut(s) 95, 98, 132, 883, 901, 916
BsgI GTGCAG 2 cut(s) 131, 923
Bsh1285I CGRYCG 1 cut(s) 29
BshFI GGCC 1 cut(s) 879
BshNI GGYRCC 2 cut(s) 308, 682
BsiEI CGRYCG 1 cut(s) 29
BsiHKAI GWGCWC 3 cut(s) 108, 322, 694
BsiSI CCGG 4 cut(s) 220, 239, 302, 893
BslFI GGGAC 1 cut(s) 16
BslI CCNNNNNNNGG 5 cut(s) 24, 39, 225, 527, 927
BsmAI GTCTC 2 cut(s) 162, 279
BsmFI GGGAC 1 cut(s) 16
BsmI GAATGC 2 cut(s) 385, 829
BsnI GGCC 1 cut(s) 879
Bsp1286I GDGCHC 4 cut(s) 108, 322, 694, 813
Bsp13I TCCGGA 1 cut(s) 238
Bsp143I GATC 5 cut(s) 45, 163, 235, 255, 798
Bsp1720I GCTNAGC 1 cut(s) 885
BspACI CCGC 6 cut(s) 40, 60, 718, 851, 921, 928
BspANI GGCC 1 cut(s) 879
BspCNI CTCAG 2 cut(s) 697, 898
BspEI TCCGGA 1 cut(s) 238
BspHI TCATGA 1 cut(s) 819
BspLI GGNNCC 3 cut(s) 237, 310, 684
BspMAI CTGCAG 1 cut(s) 901
BspPI GGATC 4 cut(s) 230, 243, 250, 793
BspT107I GGYRCC 2 cut(s) 308, 682
BsrDI GCAATG 1 cut(s) 846
BsrFI RCCGGY 2 cut(s) 301, 892
BsrI ACTGG 3 cut(s) 34, 259, 695
BssAI RCCGGY 2 cut(s) 301, 892
BssECI CCNNGG 3 cut(s) 476, 596, 614
BssMI GATC 5 cut(s) 45, 163, 235, 255, 798
BssSI CACGAG 1 cut(s) 522
Bst2BI CACGAG 1 cut(s) 522
Bst2UI CCWGG 3 cut(s) 477, 597, 633
Bst4CI ACNGT 3 cut(s) 79, 344, 661
Bst6I CTCTTC 2 cut(s) 218, 387
BstC8I GCNNGC 6 cut(s) 195, 303, 322, 681, 877, 894
BstDEI CTNAG 3 cut(s) 153, 705, 885
BstF5I GGATG 5 cut(s) 455, 541, 565, 795, 868
BstH2I RGCGCY 1 cut(s) 8
BstHHI GCGC 1 cut(s) 7
BstKTI GATC 5 cut(s) 48, 166, 238, 258, 801
BstMAI GTCTC 2 cut(s) 162, 279
BstMBI GATC 5 cut(s) 45, 163, 235, 255, 798
BstMCI CGRYCG 1 cut(s) 29
BstMWI GCNNNNNNNGC 2 cut(s) 689, 881
BstNI CCWGG 3 cut(s) 477, 597, 633
BstNSI RCATGY 1 cut(s) 606
BstSCI CCNGG 4 cut(s) 218, 475, 595, 631
BstSFI CTRYAG 3 cut(s) 75, 439, 897
BstV1I GCAGC 6 cut(s) 95, 98, 132, 883, 901, 916
BstV2I GAAGAC 1 cut(s) 509
BstX2I RGATCY 2 cut(s) 235, 255
BstYI RGATCY 2 cut(s) 235, 255
BsuRI GGCC 1 cut(s) 879
BtrI CACGTC 1 cut(s) 333
BtsCI GGATG 5 cut(s) 455, 541, 565, 795, 868
BtsI GCAGTG 2 cut(s) 413, 906
BtsIMutI CAGTG 5 cut(s) 41, 322, 413, 702, 906
Cac8I GCNNGC 6 cut(s) 195, 303, 322, 681, 877, 894
CciI TCATGA 1 cut(s) 819
CfoI GCGC 1 cut(s) 7
Cfr10I RCCGGY 2 cut(s) 301, 892
Cfr13I GGNCC 1 cut(s) 642
CseI GACGC 1 cut(s) 451
Csp6I GTAC 3 cut(s) 309, 752, 793
CviAII CATG 3 cut(s) 603, 820, 847
CviQI GTAC 3 cut(s) 309, 752, 793
DdeI CTNAG 3 cut(s) 153, 705, 885
DpnI GATC 5 cut(s) 47, 165, 237, 257, 800
DpnII GATC 5 cut(s) 45, 163, 235, 255, 798
Eam1104I CTCTTC 2 cut(s) 218, 387
EarI CTCTTC 2 cut(s) 218, 387
EciI GGCGGA 2 cut(s) 49, 866
Ecl136II GAGCTC 2 cut(s) 106, 692
Eco24I GRGCYC 3 cut(s) 108, 694, 813
Eco47I GGWCC 1 cut(s) 642
Eco53kI GAGCTC 2 cut(s) 106, 692
Eco57I CTGAAG 2 cut(s) 298, 443
EcoICRI GAGCTC 2 cut(s) 106, 692
EcoRII CCWGG 3 cut(s) 475, 595, 631
EcoT38I GRGCYC 3 cut(s) 108, 694, 813
FaeI CATG 3 cut(s) 606, 823, 850
FaiI YATR 9 cut(s) 172, 441, 489, 551, 588, 604, 610, 821, 848
FaqI GGGAC 1 cut(s) 16
FatI CATG 3 cut(s) 602, 819, 846
FauI CCCGC 1 cut(s) 711
FblI GTMKAC 1 cut(s) 71
Fnu4HI GCNGC 6 cut(s) 84, 87, 146, 890, 897, 905
FokI GGATG 5 cut(s) 442, 548, 572, 802, 855
FriOI GRGCYC 3 cut(s) 108, 694, 813
Fsp4HI GCNGC 6 cut(s) 84, 87, 146, 890, 897, 905
FspBI CTAG 1 cut(s) 134
GlaI GCGC 1 cut(s) 6
GluI GCNGC 6 cut(s) 84, 87, 146, 890, 897, 905
GsuI CTGGAG 1 cut(s) 678
HaeII RGCGCY 1 cut(s) 8
HaeIII GGCC 1 cut(s) 879
HapII CCGG 4 cut(s) 220, 239, 302, 893
HgaI GACGC 1 cut(s) 451
HhaI GCGC 1 cut(s) 7
Hin1II CATG 3 cut(s) 606, 823, 850
Hin6I GCGC 1 cut(s) 5
HinP1I GCGC 1 cut(s) 5
HinfI GANTC 7 cut(s) 242, 248, 287, 337, 352, 469, 712
HpaII CCGG 4 cut(s) 220, 239, 302, 893
HphI GGTGA 3 cut(s) 192, 740, 755
Hpy166II GTNNAC 1 cut(s) 72
Hpy188I TCNGA 5 cut(s) 100, 235, 247, 292, 351
Hpy188III TCNNGA 6 cut(s) 109, 134, 161, 239, 466, 820
Hpy8I GTNNAC 1 cut(s) 72
Hpy99I CGWCG 2 cut(s) 122, 214
HpyAV CCTTC 5 cut(s) 131, 322, 394, 418, 734
HpyCH4III ACNGT 3 cut(s) 79, 344, 661
HpyCH4IV ACGT 2 cut(s) 209, 332
HpyCH4V TGCA 4 cut(s) 148, 413, 899, 904
HpyF10VI GCNNNNNNNGC 2 cut(s) 689, 881
HpyF3I CTNAG 3 cut(s) 153, 705, 885
HpySE526I ACGT 2 cut(s) 209, 332
Hsp92II CATG 3 cut(s) 606, 823, 850
HspAI GCGC 1 cut(s) 5
Kpn2I TCCGGA 1 cut(s) 238
KpnI GGTACC 1 cut(s) 312
KroI GCCGGC 2 cut(s) 301, 892
KroNI GCCGGC 2 cut(s) 303, 894
Kzo9I GATC 5 cut(s) 45, 163, 235, 255, 798
LmnI GCTCC 1 cut(s) 697
Lsp1109I GCAGC 6 cut(s) 95, 98, 132, 883, 901, 916
LweI GCATC 1 cut(s) 206
MaeI CTAG 1 cut(s) 134
MaeII ACGT 2 cut(s) 209, 332
MalI GATC 5 cut(s) 47, 165, 237, 257, 800
MboI GATC 5 cut(s) 45, 163, 235, 255, 798
MboII GAAGA 5 cut(s) 40, 235, 404, 509, 679
MflI RGATCY 2 cut(s) 235, 255
MhlI GDGCHC 4 cut(s) 108, 322, 694, 813
MluCI AATT 4 cut(s) 407, 564, 583, 747
MlyI GAGTC 3 cut(s) 242, 281, 346
MroI TCCGGA 1 cut(s) 238
MroNI GCCGGC 2 cut(s) 301, 892
MseI TTAA 4 cut(s) 326, 567, 582, 648
MslI CAYNNNNRTG 2 cut(s) 558, 818
MspA1I CMGCKG 1 cut(s) 923
MspI CCGG 4 cut(s) 220, 239, 302, 893
MspR9I CCNGG 4 cut(s) 220, 477, 597, 633
Mva1269I GAATGC 2 cut(s) 385, 829
MvaI CCWGG 3 cut(s) 477, 597, 633
MwoI GCNNNNNNNGC 2 cut(s) 689, 881
NaeI GCCGGC 2 cut(s) 303, 894
NciI CCSGG 1 cut(s) 220
NdeII GATC 5 cut(s) 45, 163, 235, 255, 798
NgoMIV GCCGGC 2 cut(s) 301, 892
NlaIII CATG 3 cut(s) 606, 823, 850
NlaIV GGNNCC 3 cut(s) 237, 310, 684
NmeAIII GCCGAG 1 cut(s) 595
NspI RCATGY 1 cut(s) 606
PagI TCATGA 1 cut(s) 819
PctI GAATGC 2 cut(s) 385, 829
PdiI GCCGGC 2 cut(s) 303, 894
PfeI GAWTC 4 cut(s) 242, 337, 469, 712
PfoI TCCNGGA 1 cut(s) 631
PkrI GCNGC 6 cut(s) 85, 88, 147, 891, 898, 906
PleI GAGTC 3 cut(s) 242, 281, 346
PpsI GAGTC 3 cut(s) 242, 281, 346
Psp124BI GAGCTC 2 cut(s) 108, 694
Psp6I CCWGG 3 cut(s) 475, 595, 631
PspGI CCWGG 3 cut(s) 475, 595, 631
PspN4I GGNNCC 3 cut(s) 237, 310, 684
PspPI GGNCC 1 cut(s) 642
PstI CTGCAG 1 cut(s) 901
PsuI RGATCY 2 cut(s) 235, 255
RsaI GTAC 3 cut(s) 310, 753, 794
RsaNI GTAC 3 cut(s) 309, 752, 793
RseI CAYNNNNRTG 2 cut(s) 558, 818
SacI GAGCTC 2 cut(s) 108, 694
SaqAI TTAA 4 cut(s) 326, 567, 582, 648
SatI GCNGC 6 cut(s) 84, 87, 146, 890, 897, 905
Sau3AI GATC 5 cut(s) 45, 163, 235, 255, 798
Sau96I GGNCC 1 cut(s) 642
SchI GAGTC 3 cut(s) 242, 281, 346
ScrFI CCNGG 4 cut(s) 220, 477, 597, 633
SduI GDGCHC 4 cut(s) 108, 322, 694, 813
SfaNI GCATC 1 cut(s) 206
SfcI CTRYAG 3 cut(s) 75, 439, 897
SinI GGWCC 1 cut(s) 642
SmiMI CAYNNNNRTG 2 cut(s) 558, 818
SmlI CTYRAG 1 cut(s) 834
SmoI CTYRAG 1 cut(s) 834
Sse9I AATT 4 cut(s) 407, 564, 583, 747
SsiI CCGC 6 cut(s) 40, 60, 718, 851, 921, 928
SspI AATATT 1 cut(s) 785
SspMI CTAG 1 cut(s) 134
SstI GAGCTC 2 cut(s) 108, 694
StyD4I CCNGG 4 cut(s) 218, 475, 595, 631
TaaI ACNGT 3 cut(s) 79, 344, 661
TaiI ACGT 2 cut(s) 212, 335
TaqI TCGA 1 cut(s) 374
TaqII GACCGA 2 cut(s) 15, 119
TasI AATT 4 cut(s) 407, 564, 583, 747
TfiI GAWTC 4 cut(s) 242, 337, 469, 712
Tru1I TTAA 4 cut(s) 326, 567, 582, 648
Tru9I TTAA 4 cut(s) 326, 567, 582, 648
TscAI CASTG 5 cut(s) 41, 322, 420, 702, 906
TseI GCWGC 6 cut(s) 83, 86, 145, 889, 896, 904
TspDTI ATGAA 5 cut(s) 260, 576, 638, 836, 857
TspGWI ACGGA 1 cut(s) 572
TspRI CASTG 5 cut(s) 41, 322, 420, 702, 906
VpaK11BI GGWCC 1 cut(s) 642
XapI RAATTY 1 cut(s) 407
XbaI TCTAGA 1 cut(s) 133
XceI RCATGY 1 cut(s) 606
XmiI GTMKAC 1 cut(s) 71
XspI CTAG 1 cut(s) 134
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.