MD12G1263300.v1.1

ER membrane protein complex subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0015492
Physical Location & Seq
Reverse (-)
32971194 .. 32974202
3009 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1263300.v1.1.491

Sequence Viewer

Length: 939 bp
ATGGCGCTCTCTCTCTCCTCCTGTCAGTCGTCCCACTGGCGGTTGATCTTCGTAATCTCCGCCGTGCTCTGTTTTACAGTAGCAGCAGCTTTCCAATCCGATGAGCTCCTGGTGGACGACGAGGAGTTCGGTCTAGAAGGAGGGCTGCACACCAAGTCTCCTGATCTCACATTCACACGATCCACGCCTCCGCCACCATCAACAACTGCATCGACGACCCGGAAGAGGTTTTCGGATCCGGATTCGGACTCAAAGATCCAGTTCCAGCTCCATCACGCCTTTGGGGACTCTGATTTCTCGCCGGCTGGTACCTTCAGTGCTCGCTTAAAGACGTGGAATCACGGTGGTCAGACACTCACAAAGCTGCGGTTTTCGAGGAATGCCTTTACCGAAGAGGAGAAGGAGAAGTTTGCATTGCTATTGAAGGGAGATGACTTCTATAGGATAAGATTGCCATCAAGTGTTTTGAATCCTCCTGGGAGGGATTATGTCATTTCGTCAGTAAAAGCGAGATGTCTTCCACGAGAGGGTTTGGATGAGCATTTTGTTATACACACGGATGGAACTAACATTTTGGCAGTTAATTATGGTTCTCCTGGGGCATGTCCATACCCTCGGCAAATGAAACTTCCTGAAAAATGGTCCTTTAACTCTCACACGGTTTTGAAGAATAGTGAGCAGGCACCAAGAGCTCCAGTGTTTGCTGAGGATATTCTTGGGGTTGGGGGTGAGGTAGGAGATGGTGAAATTATACCACCACCAGAAAAGTCTTTTTGGGCTAAATATTGGATGTACGCGATCCCTCTTGGGCTCATTGTCATGAATGCCATTACCCAAGCAATGAACATGGCGGAGGAGGGTGCTGGTCAGCCAGCTGGTCAGGCTCAGCAGCCAGCTGCAGTCCAGCGTGGGTCAAGCTCTGCTGTGCGAAGGCGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

313

Amino Acids

34.31

Weight (kDa)

6.46

Isoelectric Point (pI)

53.12

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015492)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G65270 AT1G65270 AT1G65270
fragaria_vesca FvH4_6g00240
malus_domestica MD04G1246900.v1.1 MD12G1263300.v1.1
prunus_persica Prupe.6G363100_v2.0.a1
pyrus_communis pycom04g21760 pycom12g24060
rosa_chinensis RchiOBHm_Chr3g0447611
rosa_laevigata RLG00000025984
rosa_multiflora Rmu_sc0003550.1_g000004
rosa_roxburghii Rroxscaffold_6G00425820
rosa_rugosa Rorug02G0603500
rosa_samantha Rh3AG003700
rosa_wichuraiana Rw3G000260

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 308
AccB1I GGYRCC 2 cut(s) 308, 682
AccII CGCG 1 cut(s) 797
AccIII TCCGGA 1 cut(s) 238
AciI CCGC 5 cut(s) 40, 60, 191, 367, 851
AclWI GGATC 5 cut(s) 174, 230, 243, 250, 793
AcuI CTGAAG 1 cut(s) 298
AfaI GTAC 2 cut(s) 310, 794
AfiI CCNNNNNNNGG 3 cut(s) 39, 225, 527
AgsI TTSAA 3 cut(s) 424, 469, 667
AjiI CACGTC 1 cut(s) 333
AjnI CCWGG 3 cut(s) 108, 475, 595
AluBI AGCT 8 cut(s) 89, 106, 268, 364, 692, 875, 896, 918
AluI AGCT 8 cut(s) 89, 106, 268, 364, 692, 875, 896, 918
Alw21I GWGCWC 4 cut(s) 69, 108, 322, 694
Alw26I GTCTC 1 cut(s) 162
AlwI GGATC 5 cut(s) 174, 230, 243, 250, 793
Aor13HI TCCGGA 1 cut(s) 238
ApeKI GCWGC 6 cut(s) 83, 86, 145, 364, 889, 896
ArsI GACNNNNNNTTYG 2 cut(s) 110, 142
Asp718I GGTACC 1 cut(s) 308
AspLEI GCGC 2 cut(s) 7, 936
AspS9I GGNCC 1 cut(s) 642
AsuC2I CCSGG 1 cut(s) 220
AsuHPI GGTGA 2 cut(s) 740, 755
AvaII GGWCC 1 cut(s) 642
BamHI GGATCC 1 cut(s) 235
BanI GGYRCC 2 cut(s) 308, 682
BanII GRGCYC 3 cut(s) 108, 694, 813
BauI CACGAG 1 cut(s) 522
BbsI GAAGAC 1 cut(s) 509
Bbv12I GWGCWC 4 cut(s) 69, 108, 322, 694
BbvCI CCTCAGC 1 cut(s) 705
BbvI GCAGC 6 cut(s) 95, 98, 132, 351, 883, 901
BccI CCATC 5 cut(s) 205, 279, 463, 554, 734
BceAI ACGGC 1 cut(s) 47
BciT130I CCWGG 3 cut(s) 110, 477, 597
BcnI CCSGG 1 cut(s) 220
BcoDI GTCTC 1 cut(s) 162
BfaI CTAG 2 cut(s) 134, 937
BfmI CTRYAG 2 cut(s) 439, 897
BfoI RGCGCY 2 cut(s) 8, 937
BisI GCNGC 6 cut(s) 84, 87, 146, 365, 890, 897
BlpI GCTNAGC 1 cut(s) 885
BlsI GCNGC 6 cut(s) 85, 88, 147, 366, 891, 898
Bme1390I CCNGG 4 cut(s) 110, 220, 477, 597
Bme18I GGWCC 1 cut(s) 642
BmgBI CACGTC 1 cut(s) 333
BmgT120I GGNCC 1 cut(s) 642
BmiI GGNNCC 3 cut(s) 237, 310, 684
BmrFI CCNGG 4 cut(s) 110, 220, 477, 597
BmsI GCATC 1 cut(s) 218
BpiI GAAGAC 1 cut(s) 509
BpmI CTGGAG 1 cut(s) 678
Bpu10I CCTNAGC 1 cut(s) 705
Bpu1102I GCTNAGC 1 cut(s) 885
BpuMI CCSGG 1 cut(s) 220
BsaBI GATNNNNATC 1 cut(s) 454
BsaJI CCNNGG 3 cut(s) 476, 596, 614
BsaWI WCCGGW 1 cut(s) 238
BsaXI ACNNNNNCTCC 2 cut(s) 142, 172
Bsc4I CCNNNNNNNGG 3 cut(s) 39, 225, 527
Bse118I RCCGGY 1 cut(s) 301
Bse1I ACTGG 3 cut(s) 41, 259, 695
Bse3DI GCAATG 2 cut(s) 413, 846
Bse8I GATNNNNATC 1 cut(s) 454
BseAI TCCGGA 1 cut(s) 238
BseBI CCWGG 3 cut(s) 110, 477, 597
BseDI CCNNGG 3 cut(s) 476, 596, 614
BseGI GGATG 3 cut(s) 541, 565, 795
BseJI GATNNNNATC 1 cut(s) 454
BseLI CCNNNNNNNGG 3 cut(s) 39, 225, 527
BseMI GCAATG 2 cut(s) 413, 846
BseMII CTCAG 2 cut(s) 696, 899
BseNI ACTGG 3 cut(s) 41, 259, 695
BseRI GAGGAG 4 cut(s) 7, 137, 410, 869
BseXI GCAGC 6 cut(s) 95, 98, 132, 351, 883, 901
BsgI GTGCAG 1 cut(s) 131
Bsh1236I CGCG 1 cut(s) 797
BshNI GGYRCC 2 cut(s) 308, 682
BsiHKAI GWGCWC 4 cut(s) 69, 108, 322, 694
BsiSI CCGG 3 cut(s) 220, 239, 302
BslFI GGGAC 2 cut(s) 16, 299
BslI CCNNNNNNNGG 3 cut(s) 39, 225, 527
BsmAI GTCTC 1 cut(s) 162
BsmFI GGGAC 2 cut(s) 16, 299
BsmI GAATGC 2 cut(s) 385, 829
Bsp1286I GDGCHC 5 cut(s) 69, 108, 322, 694, 813
Bsp13I TCCGGA 1 cut(s) 238
Bsp143I GATC 6 cut(s) 45, 163, 179, 235, 255, 798
Bsp1720I GCTNAGC 1 cut(s) 885
BspACI CCGC 5 cut(s) 40, 60, 191, 367, 851
BspCNI CTCAG 2 cut(s) 697, 898
BspEI TCCGGA 1 cut(s) 238
BspFNI CGCG 1 cut(s) 797
BspHI TCATGA 1 cut(s) 819
BspLI GGNNCC 3 cut(s) 237, 310, 684
BspMAI CTGCAG 1 cut(s) 901
BspPI GGATC 5 cut(s) 174, 230, 243, 250, 793
BspT107I GGYRCC 2 cut(s) 308, 682
BsrDI GCAATG 2 cut(s) 413, 846
BsrFI RCCGGY 1 cut(s) 301
BsrI ACTGG 3 cut(s) 41, 259, 695
BssAI RCCGGY 1 cut(s) 301
BssECI CCNNGG 3 cut(s) 476, 596, 614
BssMI GATC 6 cut(s) 45, 163, 179, 235, 255, 798
BssSI CACGAG 1 cut(s) 522
Bst2BI CACGAG 1 cut(s) 522
Bst2UI CCWGG 3 cut(s) 110, 477, 597
Bst4CI ACNGT 3 cut(s) 79, 344, 661
Bst6I CTCTTC 2 cut(s) 218, 387
BstC8I GCNNGC 5 cut(s) 303, 322, 681, 873, 894
BstDEI CTNAG 2 cut(s) 705, 885
BstF5I GGATG 3 cut(s) 541, 565, 795
BstFNI CGCG 1 cut(s) 797
BstH2I RGCGCY 2 cut(s) 8, 937
BstHHI GCGC 2 cut(s) 7, 936
BstKTI GATC 6 cut(s) 48, 166, 182, 238, 258, 801
BstMAI GTCTC 1 cut(s) 162
BstMBI GATC 6 cut(s) 45, 163, 179, 235, 255, 798
BstMWI GCNNNNNNNGC 2 cut(s) 689, 881
BstNI CCWGG 3 cut(s) 110, 477, 597
BstNSI RCATGY 1 cut(s) 606
BstSCI CCNGG 4 cut(s) 108, 218, 475, 595
BstSFI CTRYAG 2 cut(s) 439, 897
BstUI CGCG 1 cut(s) 797
BstV1I GCAGC 6 cut(s) 95, 98, 132, 351, 883, 901
BstV2I GAAGAC 1 cut(s) 509
BstX2I RGATCY 2 cut(s) 235, 255
BstYI RGATCY 2 cut(s) 235, 255
BtrI CACGTC 1 cut(s) 333
BtsCI GGATG 3 cut(s) 541, 565, 795
BtsIMutI CAGTG 3 cut(s) 34, 322, 702
Cac8I GCNNGC 5 cut(s) 303, 322, 681, 873, 894
CciI TCATGA 1 cut(s) 819
CfoI GCGC 2 cut(s) 7, 936
Cfr10I RCCGGY 1 cut(s) 301
Cfr13I GGNCC 1 cut(s) 642
Csp6I GTAC 2 cut(s) 309, 793
CviAII CATG 3 cut(s) 603, 820, 847
CviQI GTAC 2 cut(s) 309, 793
DdeI CTNAG 2 cut(s) 705, 885
DpnI GATC 6 cut(s) 47, 165, 181, 237, 257, 800
DpnII GATC 6 cut(s) 45, 163, 179, 235, 255, 798
Eam1104I CTCTTC 2 cut(s) 218, 387
EarI CTCTTC 2 cut(s) 218, 387
EciI GGCGGA 3 cut(s) 49, 180, 866
Ecl136II GAGCTC 2 cut(s) 106, 692
Eco24I GRGCYC 3 cut(s) 108, 694, 813
Eco47I GGWCC 1 cut(s) 642
Eco53kI GAGCTC 2 cut(s) 106, 692
Eco57I CTGAAG 1 cut(s) 298
EcoICRI GAGCTC 2 cut(s) 106, 692
EcoRII CCWGG 3 cut(s) 108, 475, 595
EcoT38I GRGCYC 3 cut(s) 108, 694, 813
FaeI CATG 3 cut(s) 606, 823, 850
FaiI YATR 9 cut(s) 441, 489, 551, 588, 604, 610, 752, 821, 848
FaqI GGGAC 2 cut(s) 16, 299
FatI CATG 3 cut(s) 602, 819, 846
Fnu4HI GCNGC 6 cut(s) 84, 87, 146, 365, 890, 897
FokI GGATG 3 cut(s) 548, 572, 802
FriOI GRGCYC 3 cut(s) 108, 694, 813
Fsp4HI GCNGC 6 cut(s) 84, 87, 146, 365, 890, 897
FspBI CTAG 2 cut(s) 134, 937
GlaI GCGC 2 cut(s) 6, 935
GluI GCNGC 6 cut(s) 84, 87, 146, 365, 890, 897
GsuI CTGGAG 1 cut(s) 678
HaeII RGCGCY 2 cut(s) 8, 937
HapII CCGG 3 cut(s) 220, 239, 302
HhaI GCGC 2 cut(s) 7, 936
Hin1II CATG 3 cut(s) 606, 823, 850
Hin6I GCGC 2 cut(s) 5, 934
HinP1I GCGC 2 cut(s) 5, 934
HinfI GANTC 5 cut(s) 242, 248, 287, 337, 469
HpaII CCGG 3 cut(s) 220, 239, 302
HphI GGTGA 2 cut(s) 740, 755
Hpy166II GTNNAC 1 cut(s) 115
Hpy188I TCNGA 5 cut(s) 100, 235, 247, 292, 351
Hpy188III TCNNGA 5 cut(s) 134, 161, 239, 632, 820
Hpy8I GTNNAC 1 cut(s) 115
Hpy99I CGWCG 2 cut(s) 122, 217
HpyAV CCTTC 5 cut(s) 131, 322, 394, 418, 924
HpyCH4III ACNGT 3 cut(s) 79, 344, 661
HpyCH4IV ACGT 1 cut(s) 332
HpyCH4V TGCA 4 cut(s) 148, 209, 413, 899
HpyF10VI GCNNNNNNNGC 2 cut(s) 689, 881
HpyF3I CTNAG 2 cut(s) 705, 885
HpySE526I ACGT 1 cut(s) 332
Hsp92II CATG 3 cut(s) 606, 823, 850
HspAI GCGC 2 cut(s) 5, 934
Kpn2I TCCGGA 1 cut(s) 238
KpnI GGTACC 1 cut(s) 312
KroI GCCGGC 1 cut(s) 301
KroNI GCCGGC 1 cut(s) 303
Kzo9I GATC 6 cut(s) 45, 163, 179, 235, 255, 798
LmnI GCTCC 3 cut(s) 111, 273, 697
Lsp1109I GCAGC 6 cut(s) 95, 98, 132, 351, 883, 901
LweI GCATC 1 cut(s) 218
MaeI CTAG 2 cut(s) 134, 937
MaeII ACGT 1 cut(s) 332
MalI GATC 6 cut(s) 47, 165, 181, 237, 257, 800
MboI GATC 6 cut(s) 45, 163, 179, 235, 255, 798
MboII GAAGA 5 cut(s) 40, 235, 404, 509, 679
MflI RGATCY 2 cut(s) 235, 255
MhlI GDGCHC 5 cut(s) 69, 108, 322, 694, 813
MluCI AATT 2 cut(s) 583, 747
MlyI GAGTC 2 cut(s) 242, 281
MroI TCCGGA 1 cut(s) 238
MroNI GCCGGC 1 cut(s) 301
MseI TTAA 3 cut(s) 326, 582, 648
MslI CAYNNNNRTG 2 cut(s) 558, 818
MspA1I CMGCKG 2 cut(s) 875, 896
MspI CCGG 3 cut(s) 220, 239, 302
MspR9I CCNGG 4 cut(s) 110, 220, 477, 597
Mva1269I GAATGC 2 cut(s) 385, 829
MvaI CCWGG 3 cut(s) 110, 477, 597
MvnI CGCG 1 cut(s) 797
MwoI GCNNNNNNNGC 2 cut(s) 689, 881
NaeI GCCGGC 1 cut(s) 303
NciI CCSGG 1 cut(s) 220
NdeII GATC 6 cut(s) 45, 163, 179, 235, 255, 798
NgoMIV GCCGGC 1 cut(s) 301
NlaIII CATG 3 cut(s) 606, 823, 850
NlaIV GGNNCC 3 cut(s) 237, 310, 684
NmeAIII GCCGAG 1 cut(s) 595
NspI RCATGY 1 cut(s) 606
PagI TCATGA 1 cut(s) 819
PctI GAATGC 2 cut(s) 385, 829
PdiI GCCGGC 1 cut(s) 303
PfeI GAWTC 3 cut(s) 242, 337, 469
PkrI GCNGC 6 cut(s) 85, 88, 147, 366, 891, 898
PleI GAGTC 2 cut(s) 242, 281
PpsI GAGTC 2 cut(s) 242, 281
Psp124BI GAGCTC 2 cut(s) 108, 694
Psp6I CCWGG 3 cut(s) 108, 475, 595
PspGI CCWGG 3 cut(s) 108, 475, 595
PspN4I GGNNCC 3 cut(s) 237, 310, 684
PspPI GGNCC 1 cut(s) 642
PstI CTGCAG 1 cut(s) 901
PsuI RGATCY 2 cut(s) 235, 255
PvuII CAGCTG 2 cut(s) 875, 896
RsaI GTAC 2 cut(s) 310, 794
RsaNI GTAC 2 cut(s) 309, 793
RseI CAYNNNNRTG 2 cut(s) 558, 818
SacI GAGCTC 2 cut(s) 108, 694
SaqAI TTAA 3 cut(s) 326, 582, 648
SatI GCNGC 6 cut(s) 84, 87, 146, 365, 890, 897
Sau3AI GATC 6 cut(s) 45, 163, 179, 235, 255, 798
Sau96I GGNCC 1 cut(s) 642
SchI GAGTC 2 cut(s) 242, 281
ScrFI CCNGG 4 cut(s) 110, 220, 477, 597
SduI GDGCHC 5 cut(s) 69, 108, 322, 694, 813
SfaNI GCATC 1 cut(s) 218
SfcI CTRYAG 2 cut(s) 439, 897
SinI GGWCC 1 cut(s) 642
SmiMI CAYNNNNRTG 2 cut(s) 558, 818
Sse9I AATT 2 cut(s) 583, 747
SsiI CCGC 5 cut(s) 40, 60, 191, 367, 851
SspI AATATT 1 cut(s) 785
SspMI CTAG 2 cut(s) 134, 937
SstI GAGCTC 2 cut(s) 108, 694
StyD4I CCNGG 4 cut(s) 108, 218, 475, 595
TaaI ACNGT 3 cut(s) 79, 344, 661
TaiI ACGT 1 cut(s) 335
TaqI TCGA 2 cut(s) 212, 374
TaqII GACCGA 1 cut(s) 119
TasI AATT 2 cut(s) 583, 747
TfiI GAWTC 3 cut(s) 242, 337, 469
Tru1I TTAA 3 cut(s) 326, 582, 648
Tru9I TTAA 3 cut(s) 326, 582, 648
TscAI CASTG 3 cut(s) 41, 322, 702
TseI GCWGC 6 cut(s) 83, 86, 145, 364, 889, 896
TspDTI ATGAA 3 cut(s) 638, 836, 857
TspGWI ACGGA 1 cut(s) 572
TspRI CASTG 3 cut(s) 41, 322, 702
VpaK11BI GGWCC 1 cut(s) 642
XbaI TCTAGA 1 cut(s) 133
XceI RCATGY 1 cut(s) 606
XcmI CCANNNNNNNNNTGG 1 cut(s) 278
XspI CTAG 2 cut(s) 134, 937
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.