AT1G65820

Microsomal glutathione S-transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0004632
Physical Location & Seq
Forward (+)
24485046 .. 24486954
1909 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G65820.1

Sequence Viewer

Length: 441 bp
ATGGCGGCGATTACAGAATTTCTACCAAAAGAGTACGGATATGTCGTTCTCGTCCTCGTCTTCTACTGTTTCCTCAACCTCTGGATGGGTGCTCAAGTCGGCAGAGCTCGCAAAAGGTACAACGTCCCGTATCCAACTCTATATGCAATAGAATCAGAAAACAAAGATGCTAAGCTCTTCAACTGTGTTCAGAGAGGACATCAAAACTCTTTAGAGATGATGCCAATGTATTTCATACTGATGATCCTCGGTGGGATGAAGCACCCTTGTATCTGTACTGGCCTTGGTTTGCTTTACAACGTTAGCCGATTCTTCTACTTTAAAGGTTATGCTACTGGAGATCCCATGAAGCGTCTTACGATCGGGAAATACGGTTTCTTGGGGTTGCTAGGTCTGATGATATGTACCATCTCGTTTGGTGTCACTCTGATCCTTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00480 ko00980 ko00982 ko00983 ko01524 ko05200 ko05204 ko05225 ko05418 map00480 map00980 map00982 map00983 map01524 map05200 map05204 map05225 map05418
Pfam Domains
Protein Families
MAPEG

Protein Analysis

146

Amino Acids

16.59

Weight (kDa)

9.24

Isoelectric Point (pI)

41.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MAPEG PF01124 16 - 137 6e-28 MAPEG family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 5
AclI AACGTT 1 cut(s) 300
AclWI GGATC 3 cut(s) 238, 335, 424
AcsI RAATTY 1 cut(s) 17
AfaI GTAC 4 cut(s) 35, 119, 277, 406
AfiI CCNNNNNNNGG 1 cut(s) 85
AgsI TTSAA 1 cut(s) 181
AloI GAACNNNNNNTCC 2 cut(s) 30, 62
AluBI AGCT 2 cut(s) 107, 175
AluI AGCT 2 cut(s) 107, 175
Alw21I GWGCWC 2 cut(s) 94, 109
AlwI GGATC 3 cut(s) 238, 335, 424
AoxI GGCC 1 cut(s) 280
ApoI RAATTY 1 cut(s) 17
BaeI ACNNNNGTAYC 2 cut(s) 253, 286
BanII GRGCYC 1 cut(s) 109
BbsI GAAGAC 1 cut(s) 52
Bbv12I GWGCWC 2 cut(s) 94, 109
BccI CCATC 2 cut(s) 79, 416
BciVI GTATCC 1 cut(s) 141
BfaI CTAG 1 cut(s) 389
BfuI GTATCC 1 cut(s) 141
BisI GCNGC 1 cut(s) 6
BlpI GCTNAGC 1 cut(s) 171
BlsI GCNGC 1 cut(s) 7
BmsI GCATC 2 cut(s) 157, 210
BpiI GAAGAC 1 cut(s) 52
BpmI CTGGAG 1 cut(s) 357
Bpu1102I GCTNAGC 1 cut(s) 171
BpuEI CTTGAG 1 cut(s) 78
BsaJI CCNNGG 2 cut(s) 247, 283
Bsc4I CCNNNNNNNGG 1 cut(s) 85
Bse1I ACTGG 2 cut(s) 283, 340
BseDI CCNNGG 2 cut(s) 247, 283
BseGI GGATG 2 cut(s) 90, 261
BseLI CCNNNNNNNGG 1 cut(s) 85
BseNI ACTGG 2 cut(s) 283, 340
Bsh1285I CGRYCG 1 cut(s) 363
BshFI GGCC 1 cut(s) 282
BsiEI CGRYCG 1 cut(s) 363
BsiHKAI GWGCWC 2 cut(s) 94, 109
BslFI GGGAC 1 cut(s) 110
BslI CCNNNNNNNGG 1 cut(s) 85
BsmFI GGGAC 1 cut(s) 110
BsnI GGCC 1 cut(s) 282
Bsp1286I GDGCHC 2 cut(s) 94, 109
Bsp143I GATC 4 cut(s) 243, 340, 360, 429
Bsp1720I GCTNAGC 1 cut(s) 171
BspACI CCGC 1 cut(s) 5
BspANI GGCC 1 cut(s) 282
BspPI GGATC 3 cut(s) 238, 335, 424
BspQI GCTCTTC 1 cut(s) 182
BsrI ACTGG 2 cut(s) 283, 340
BssECI CCNNGG 2 cut(s) 247, 283
BssMI GATC 4 cut(s) 243, 340, 360, 429
BssT1I CCWWGG 1 cut(s) 283
Bst4CI ACNGT 3 cut(s) 68, 185, 374
Bst6I CTCTTC 1 cut(s) 182
BstC8I GCNNGC 1 cut(s) 109
BstDEI CTNAG 1 cut(s) 171
BstF5I GGATG 2 cut(s) 90, 261
BstKTI GATC 4 cut(s) 246, 343, 363, 432
BstMBI GATC 4 cut(s) 243, 340, 360, 429
BstMCI CGRYCG 1 cut(s) 363
BstMWI GCNNNNNNNGC 1 cut(s) 108
BstV2I GAAGAC 1 cut(s) 52
BstX2I RGATCY 1 cut(s) 340
BstYI RGATCY 1 cut(s) 340
BsuI GTATCC 1 cut(s) 141
BsuRI GGCC 1 cut(s) 282
BtsCI GGATG 2 cut(s) 90, 261
Cac8I GCNNGC 1 cut(s) 109
CseI GACGC 1 cut(s) 341
Csp6I GTAC 4 cut(s) 34, 118, 276, 405
CviAII CATG 1 cut(s) 346
CviJI RGCY 4 cut(s) 107, 175, 282, 306
CviKI_1 RGCY 4 cut(s) 107, 175, 282, 306
CviQI GTAC 4 cut(s) 34, 118, 276, 405
DdeI CTNAG 1 cut(s) 171
DpnI GATC 4 cut(s) 245, 342, 362, 431
DpnII GATC 4 cut(s) 243, 340, 360, 429
DraI TTTAAA 1 cut(s) 322
Eam1104I CTCTTC 1 cut(s) 182
EarI CTCTTC 1 cut(s) 182
Ecl136II GAGCTC 1 cut(s) 107
Eco130I CCWWGG 1 cut(s) 283
Eco24I GRGCYC 1 cut(s) 109
Eco53kI GAGCTC 1 cut(s) 107
EcoICRI GAGCTC 1 cut(s) 107
EcoT14I CCWWGG 1 cut(s) 283
EcoT38I GRGCYC 1 cut(s) 109
ErhI CCWWGG 1 cut(s) 283
FaeI CATG 1 cut(s) 349
FaiI YATR 7 cut(s) 42, 142, 144, 236, 330, 347, 403
FaqI GGGAC 1 cut(s) 110
FatI CATG 1 cut(s) 345
Fnu4HI GCNGC 1 cut(s) 6
FokI GGATG 2 cut(s) 97, 268
FriOI GRGCYC 1 cut(s) 109
Fsp4HI GCNGC 1 cut(s) 6
FspBI CTAG 1 cut(s) 389
GluI GCNGC 1 cut(s) 6
GsuI CTGGAG 1 cut(s) 357
HaeIII GGCC 1 cut(s) 282
HgaI GACGC 1 cut(s) 341
Hin1II CATG 1 cut(s) 349
HinfI GANTC 2 cut(s) 152, 309
Hpy188I TCNGA 4 cut(s) 157, 192, 396, 429
Hpy188III TCNNGA 2 cut(s) 82, 364
HpyCH4III ACNGT 3 cut(s) 68, 185, 374
HpyCH4IV ACGT 2 cut(s) 123, 300
HpyCH4V TGCA 1 cut(s) 146
HpyF10VI GCNNNNNNNGC 1 cut(s) 108
HpyF3I CTNAG 1 cut(s) 171
HpySE526I ACGT 2 cut(s) 123, 300
Hsp92II CATG 1 cut(s) 349
Kzo9I GATC 4 cut(s) 243, 340, 360, 429
LguI GCTCTTC 1 cut(s) 182
LpnPI CCDG 3 cut(s) 67, 264, 321
LweI GCATC 2 cut(s) 157, 210
MaeI CTAG 1 cut(s) 389
MaeII ACGT 2 cut(s) 123, 300
MaeIII GTNAC 1 cut(s) 421
MalI GATC 4 cut(s) 245, 342, 362, 431
MboI GATC 4 cut(s) 243, 340, 360, 429
MboII GAAGA 3 cut(s) 52, 169, 304
MflI RGATCY 1 cut(s) 340
MhlI GDGCHC 2 cut(s) 94, 109
MluCI AATT 1 cut(s) 17
MmeI TCCRAC 1 cut(s) 158
MnlI CCTC 5 cut(s) 65, 83, 89, 188, 257
MseI TTAA 1 cut(s) 321
MslI CAYNNNNRTG 1 cut(s) 239
MwoI GCNNNNNNNGC 1 cut(s) 108
NdeII GATC 4 cut(s) 243, 340, 360, 429
NlaIII CATG 1 cut(s) 349
NmuCI GTSAC 1 cut(s) 421
PciSI GCTCTTC 1 cut(s) 182
PcsI WCGNNNNNNNCGW 1 cut(s) 42
PfeI GAWTC 2 cut(s) 152, 309
PkrI GCNGC 1 cut(s) 7
Ple19I CGATCG 1 cut(s) 363
Psp124BI GAGCTC 1 cut(s) 109
Psp1406I AACGTT 1 cut(s) 300
PsuI RGATCY 1 cut(s) 340
PvuI CGATCG 1 cut(s) 363
RsaI GTAC 4 cut(s) 35, 119, 277, 406
RsaNI GTAC 4 cut(s) 34, 118, 276, 405
RseI CAYNNNNRTG 1 cut(s) 239
SacI GAGCTC 1 cut(s) 109
SapI GCTCTTC 1 cut(s) 182
SaqAI TTAA 1 cut(s) 321
SatI GCNGC 1 cut(s) 6
Sau3AI GATC 4 cut(s) 243, 340, 360, 429
SduI GDGCHC 2 cut(s) 94, 109
SetI ASST 8 cut(s) 81, 109, 119, 126, 177, 303, 328, 394
SfaNI GCATC 2 cut(s) 157, 210
SmiMI CAYNNNNRTG 1 cut(s) 239
SmlI CTYRAG 1 cut(s) 93
SmoI CTYRAG 1 cut(s) 93
Sse9I AATT 1 cut(s) 17
SsiI CCGC 1 cut(s) 5
SspMI CTAG 1 cut(s) 389
SstI GAGCTC 1 cut(s) 109
StyI CCWWGG 1 cut(s) 283
TaaI ACNGT 3 cut(s) 68, 185, 374
TaiI ACGT 2 cut(s) 126, 303
TasI AATT 1 cut(s) 17
TatI WGTACW 1 cut(s) 275
TauI GCSGC 1 cut(s) 8
TfiI GAWTC 2 cut(s) 152, 309
Tru1I TTAA 1 cut(s) 321
Tru9I TTAA 1 cut(s) 321
TseFI GTSAC 1 cut(s) 421
Tsp45I GTSAC 1 cut(s) 421
TspDTI ATGAA 3 cut(s) 223, 272, 362
TspGWI ACGGA 1 cut(s) 51
XapI RAATTY 1 cut(s) 17
XspI CTAG 1 cut(s) 389
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.