MD17G1267400.v1.1

Microsomal glutathione S-transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0004632
Physical Location & Seq
Reverse (-)
32754259 .. 32756338
2080 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1267400.v1.1.491

Sequence Viewer

Length: 444 bp
ATGGCTTCTGCAGTCGAATTGCTGCCCAAAGAATACGGCTATGTAGTCCTCGCCGTCGTTCTCTACACTTTTCTCAATTTCTGCATGGCCGCCAAAGTTGGCAGGGCCAGAAAAAGGTACAAGGTGCCGTATCCGACCATGTATGCGTCGGAATCGGAGAACAAAGATGCAAAAGTGTTCAACTGTGTGCAGAGAGGCCATCAGAACTCTCTCGAAATGATGCCTATGTTGTTTGTGCTTCTGGTGTTGGGAGGTCTTAAGCATCCTTGCATCTCCGCCGCGCTTGGTTTCCTCTTCACTTTCACTCGCTATTTCTACTTCAAGGGCTACGCCACCGGTGACCCCATAAACCGCCTCGCCATCGGGAAATACGGGTTCTTGGCTATATTCGGGCTTATAGGGTGCACAATTTCGTTTGGGGTCAGTCTTCTTCTTCGAGGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00480 ko00980 ko00982 ko00983 ko01524 ko05200 ko05204 ko05225 ko05418 map00480 map00980 map00982 map00983 map01524 map05200 map05204 map05225 map05418
Pfam Domains
Protein Families
MAPEG

Protein Analysis

148

Amino Acids

16.38

Weight (kDa)

9.54

Isoelectric Point (pI)

27.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MAPEG PF01124 15 - 137 8e-27 MAPEG family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 124
AccII CGCG 1 cut(s) 281
AciI CCGC 4 cut(s) 90, 276, 279, 352
AcoI YGGCCR 1 cut(s) 87
AfaI GTAC 1 cut(s) 119
AfiI CCNNNNNNNGG 1 cut(s) 114
AflII CTTAAG 1 cut(s) 257
AgeI ACCGGT 1 cut(s) 335
AgsI TTSAA 2 cut(s) 181, 322
Alw21I GWGCWC 1 cut(s) 407
Alw44I GTGCAC 1 cut(s) 403
AoxI GGCC 3 cut(s) 87, 105, 196
ApaLI GTGCAC 1 cut(s) 403
ApeKI GCWGC 1 cut(s) 22
AsiGI ACCGGT 1 cut(s) 335
AspLEI GCGC 1 cut(s) 283
AspS9I GGNCC 1 cut(s) 105
AsuHPI GGTGA 1 cut(s) 350
BaeGI GKGCMC 1 cut(s) 407
BanI GGYRCC 1 cut(s) 124
BbsI GAAGAC 1 cut(s) 419
Bbv12I GWGCWC 1 cut(s) 407
BbvI GCAGC 1 cut(s) 9
BccI CCATC 2 cut(s) 207, 368
BceAI ACGGC 3 cut(s) 38, 52, 112
BciVI GTATCC 1 cut(s) 141
BfmI CTRYAG 1 cut(s) 9
BfrI CTTAAG 1 cut(s) 257
BfuI GTATCC 1 cut(s) 141
BisI GCNGC 3 cut(s) 23, 90, 279
BlsI GCNGC 3 cut(s) 24, 91, 280
BmgT120I GGNCC 1 cut(s) 105
BmiI GGNNCC 1 cut(s) 126
BmsI GCATC 4 cut(s) 157, 210, 271, 279
BpiI GAAGAC 1 cut(s) 419
BsaWI WCCGGW 1 cut(s) 335
Bsc4I CCNNNNNNNGG 1 cut(s) 114
Bse118I RCCGGY 1 cut(s) 335
BseGI GGATG 1 cut(s) 262
BseLI CCNNNNNNNGG 1 cut(s) 114
BseSI GKGCMC 1 cut(s) 407
BseXI GCAGC 1 cut(s) 9
BsgI GTGCAG 1 cut(s) 209
Bsh1236I CGCG 1 cut(s) 281
BshFI GGCC 3 cut(s) 89, 107, 198
BshNI GGYRCC 1 cut(s) 124
BshTI ACCGGT 1 cut(s) 335
BsiHKAI GWGCWC 1 cut(s) 407
BsiSI CCGG 1 cut(s) 336
BslI CCNNNNNNNGG 1 cut(s) 114
BsnI GGCC 3 cut(s) 89, 107, 198
Bsp1286I GDGCHC 1 cut(s) 407
BspACI CCGC 4 cut(s) 90, 276, 279, 352
BspANI GGCC 3 cut(s) 89, 107, 198
BspFNI CGCG 1 cut(s) 281
BspLI GGNNCC 1 cut(s) 126
BspMAI CTGCAG 1 cut(s) 13
BspT107I GGYRCC 1 cut(s) 124
BspTI CTTAAG 1 cut(s) 257
BsrFI RCCGGY 1 cut(s) 335
BssAI RCCGGY 1 cut(s) 335
Bst4CI ACNGT 1 cut(s) 185
Bst6I CTCTTC 1 cut(s) 299
BstAFI CTTAAG 1 cut(s) 257
BstEII GGTNACC 1 cut(s) 338
BstF5I GGATG 1 cut(s) 262
BstFNI CGCG 1 cut(s) 281
BstHHI GCGC 1 cut(s) 283
BstPI GGTNACC 1 cut(s) 338
BstSFI CTRYAG 1 cut(s) 9
BstSLI GKGCMC 1 cut(s) 407
BstUI CGCG 1 cut(s) 281
BstV1I GCAGC 1 cut(s) 9
BstV2I GAAGAC 1 cut(s) 419
BsuI GTATCC 1 cut(s) 141
BsuRI GGCC 3 cut(s) 89, 107, 198
BtsCI GGATG 1 cut(s) 262
CfoI GCGC 1 cut(s) 283
Cfr10I RCCGGY 1 cut(s) 335
Cfr13I GGNCC 1 cut(s) 105
CseI GACGC 1 cut(s) 135
Csp6I GTAC 1 cut(s) 118
CspAI ACCGGT 1 cut(s) 335
CviAII CATG 2 cut(s) 85, 139
CviJI RGCY 8 cut(s) 5, 39, 89, 107, 198, 327, 383, 394
CviKI_1 RGCY 8 cut(s) 5, 39, 89, 107, 198, 327, 383, 394
CviQI GTAC 1 cut(s) 118
EaeI YGGCCR 1 cut(s) 87
Eam1104I CTCTTC 1 cut(s) 299
EarI CTCTTC 1 cut(s) 299
EciI GGCGGA 1 cut(s) 265
Eco91I GGTNACC 1 cut(s) 338
EcoO65I GGTNACC 1 cut(s) 338
FaeI CATG 2 cut(s) 88, 142
FaiI YATR 8 cut(s) 42, 86, 140, 144, 227, 347, 386, 398
FatI CATG 2 cut(s) 84, 138
Fnu4HI GCNGC 3 cut(s) 23, 90, 279
FokI GGATG 1 cut(s) 249
Fsp4HI GCNGC 3 cut(s) 23, 90, 279
GlaI GCGC 1 cut(s) 282
GluI GCNGC 3 cut(s) 23, 90, 279
HaeIII GGCC 3 cut(s) 89, 107, 198
HapII CCGG 1 cut(s) 336
HgaI GACGC 1 cut(s) 135
HhaI GCGC 1 cut(s) 283
Hin1II CATG 2 cut(s) 88, 142
Hin6I GCGC 1 cut(s) 281
HinP1I GCGC 1 cut(s) 281
HinfI GANTC 1 cut(s) 152
HpaII CCGG 1 cut(s) 336
HphI GGTGA 1 cut(s) 350
Hpy166II GTNNAC 1 cut(s) 405
Hpy188I TCNGA 4 cut(s) 135, 151, 157, 204
Hpy188III TCNNGA 2 cut(s) 212, 364
Hpy8I GTNNAC 1 cut(s) 405
Hpy99I CGWCG 2 cut(s) 59, 151
HpyCH4III ACNGT 1 cut(s) 185
HpyCH4V TGCA 6 cut(s) 11, 84, 170, 190, 270, 405
Hsp92II CATG 2 cut(s) 88, 142
HspAI GCGC 1 cut(s) 281
LpnPI CCDG 4 cut(s) 88, 121, 227, 349
Lsp1109I GCAGC 1 cut(s) 9
LweI GCATC 4 cut(s) 157, 210, 271, 279
MaeIII GTNAC 1 cut(s) 338
MboII GAAGA 4 cut(s) 286, 419, 422, 425
MhlI GDGCHC 1 cut(s) 407
MluCI AATT 3 cut(s) 17, 76, 408
MmeI TCCRAC 2 cut(s) 129, 158
MnlI CCTC 6 cut(s) 59, 188, 245, 302, 365, 431
MseI TTAA 1 cut(s) 258
MspCI CTTAAG 1 cut(s) 257
MspI CCGG 1 cut(s) 336
MvnI CGCG 1 cut(s) 281
NlaIII CATG 2 cut(s) 88, 142
NlaIV GGNNCC 1 cut(s) 126
NmuCI GTSAC 1 cut(s) 338
PfeI GAWTC 1 cut(s) 152
PinAI ACCGGT 1 cut(s) 335
PkrI GCNGC 3 cut(s) 24, 91, 280
PspEI GGTNACC 1 cut(s) 338
PspN4I GGNNCC 1 cut(s) 126
PspPI GGNCC 1 cut(s) 105
PstI CTGCAG 1 cut(s) 13
RsaI GTAC 1 cut(s) 119
RsaNI GTAC 1 cut(s) 118
SaqAI TTAA 1 cut(s) 258
SatI GCNGC 3 cut(s) 23, 90, 279
Sau96I GGNCC 1 cut(s) 105
SduI GDGCHC 1 cut(s) 407
SetI ASST 3 cut(s) 119, 126, 256
SfaNI GCATC 4 cut(s) 157, 210, 271, 279
SfcI CTRYAG 1 cut(s) 9
SgrAI CRCCGGYG 1 cut(s) 335
SmlI CTYRAG 1 cut(s) 257
SmoI CTYRAG 1 cut(s) 257
Sse9I AATT 3 cut(s) 17, 76, 408
SsiI CCGC 4 cut(s) 90, 276, 279, 352
TaaI ACNGT 1 cut(s) 185
TaqI TCGA 3 cut(s) 15, 213, 436
TasI AATT 3 cut(s) 17, 76, 408
TauI GCSGC 2 cut(s) 92, 281
TfiI GAWTC 1 cut(s) 152
Tru1I TTAA 1 cut(s) 258
Tru9I TTAA 1 cut(s) 258
TseFI GTSAC 1 cut(s) 338
TseI GCWGC 1 cut(s) 22
Tsp45I GTSAC 1 cut(s) 338
Vha464I CTTAAG 1 cut(s) 257
VneI GTGCAC 1 cut(s) 403
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.