AT1G67400

ELMO/CED-12 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0007302
Physical Location & Seq
Reverse (-)
25247918 .. 25251191
3274 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G67400.1

Sequence Viewer

Length: 846 bp
ATGCGAGTTGTCAGAATATCATGCGTCGGGACACGTCATCTCTCACCTCCCTCCTCCGTTCGCGGTTGCGAAGCCCACTCCGACGACGTCTCTGCTTCCTCAGCCGGACTAATTAACGCGGATGCAGAAGATGCGAGTTGTAGTAGCACGCCTAGTTGGAGAATCAAGAAGAGTCTAACATGTGTTTGCTTTAACCGCAAGAGAGCATATGAACGCATTTGCTCTAACTTGACACCATTGCAGGAAGAAAGACTTAAAAGGTTGAGGAAGAGAATGAAGAATTACTATGATGCATCACGGCCCGATCATCAGGATGCATTAAGAGCACTGTGGTCAGCAACGTATCCTGATGAAAAGCTTCAAGATTTGATCTCAGACCAGTGGAAAAATATGGGATGGCAAGGAAAAGATCCATCCACTGATTTCAGAGGAGCTGGATTCATTTCACTGGAGAATCTTCTATTCTTTGCAAAGACTTTCTCGACTTCGTTTCAGCGTCTGTTGAATAAACAAGGAGGCAAAAGAGCGGCTTGGGAATATCCATTCGCTGTAGCGGGCGTCAACATAACCTTCATGATCATGCAAATGCTTGACCTTGAAGCATCAAAACCTAGGAGTTTTATACGGTTGGTGTTCTTACAAATGTTGTCAGAAAGCGAATGGGCCTTTGATTTGCTTTACTGTGTGGCCTTTGTGGTAATGGACAAGCAATGGCTGGACAAGAACGCCACCTACATGGAATTCAACGATGTGCTAAGATGTACAAGGGGGCAGCTTGAGAGAGAGCTGATGATGGATGATGTTTTCAGAATTGAGGATATGCCTTCTTTCTCTCTTCTCTCTTAG

Protein Analysis

281

Amino Acids

32.38

Weight (kDa)

8.38

Isoelectric Point (pI)

47.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ELMO_CED12 PF04727 87 - 252 4.9e-54 ELMO/CED-12 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 90
AccBSI CCGCTC 1 cut(s) 527
AccII CGCG 2 cut(s) 63, 119
AciI CCGC 5 cut(s) 63, 119, 196, 527, 554
AclWI GGATC 1 cut(s) 404
AcsI RAATTY 1 cut(s) 740
AcyI GRCGYC 2 cut(s) 87, 558
AfaI GTAC 1 cut(s) 763
AflIII ACRYGT 2 cut(s) 32, 179
AgsI TTSAA 4 cut(s) 362, 505, 599, 745
AjiI CACGTC 1 cut(s) 35
AluBI AGCT 4 cut(s) 358, 434, 775, 787
AluI AGCT 4 cut(s) 358, 434, 775, 787
Alw21I GWGCWC 1 cut(s) 328
Alw26I GTCTC 1 cut(s) 94
AlwI GGATC 1 cut(s) 404
AlwNI CAGNNNCTG 1 cut(s) 499
AoxI GGCC 3 cut(s) 299, 663, 687
ApeKI GCWGC 1 cut(s) 772
ApoI RAATTY 1 cut(s) 740
Asp700I GAANNNNTTC 1 cut(s) 357
AspA2I CCTAGG 1 cut(s) 611
AspS9I GGNCC 2 cut(s) 300, 663
AsuHPI GGTGA 1 cut(s) 36
AvrII CCTAGG 1 cut(s) 611
Bbv12I GWGCWC 1 cut(s) 328
BbvCI CCTCAGC 1 cut(s) 100
BbvI GCAGC 1 cut(s) 784
BccI CCATC 3 cut(s) 390, 421, 787
BceAI ACGGC 1 cut(s) 314
BcgI CGANNNNNNTGC 2 cut(s) 74, 108
BciVI GTATCC 1 cut(s) 354
BclI TGATCA 1 cut(s) 576
BcoDI GTCTC 1 cut(s) 94
BfaI CTAG 2 cut(s) 153, 612
BfmI CTRYAG 1 cut(s) 549
BfuI GTATCC 1 cut(s) 354
BisI GCNGC 2 cut(s) 528, 773
BlnI CCTAGG 1 cut(s) 611
BlsI GCNGC 2 cut(s) 529, 774
BmgBI CACGTC 1 cut(s) 35
BmgT120I GGNCC 2 cut(s) 300, 663
BmsI GCATC 6 cut(s) 112, 121, 280, 302, 304, 611
BpmI CTGGAG 1 cut(s) 470
Bpu10I CCTNAGC 1 cut(s) 100
BpuEI CTTGAG 1 cut(s) 797
BsaHI GRCGYC 2 cut(s) 87, 558
BsaJI CCNNGG 1 cut(s) 611
Bse1I ACTGG 2 cut(s) 379, 453
Bse3DI GCAATG 2 cut(s) 236, 716
BseDI CCNNGG 1 cut(s) 611
BseGI GGATG 5 cut(s) 127, 319, 401, 413, 802
BseMI GCAATG 2 cut(s) 236, 716
BseMII CTCAG 2 cut(s) 114, 387
BseNI ACTGG 2 cut(s) 379, 453
BseRI GAGGAG 2 cut(s) 43, 444
BseXI GCAGC 1 cut(s) 784
Bsh1236I CGCG 2 cut(s) 63, 119
BshFI GGCC 3 cut(s) 301, 665, 689
BsiHKAI GWGCWC 1 cut(s) 328
BsiSI CCGG 1 cut(s) 105
BslFI GGGAC 1 cut(s) 43
BsmAI GTCTC 1 cut(s) 94
BsmBI CGTCTC 1 cut(s) 94
BsmFI GGGAC 1 cut(s) 43
BsnI GGCC 3 cut(s) 301, 665, 689
Bsp1286I GDGCHC 1 cut(s) 328
Bsp1407I TGTACA 1 cut(s) 761
Bsp143I GATC 4 cut(s) 304, 369, 409, 576
BspACI CCGC 5 cut(s) 63, 119, 196, 527, 554
BspANI GGCC 3 cut(s) 301, 665, 689
BspCNI CTCAG 2 cut(s) 113, 386
BspFNI CGCG 2 cut(s) 63, 119
BspHI TCATGA 1 cut(s) 573
BspPI GGATC 1 cut(s) 404
BsrBI CCGCTC 1 cut(s) 527
BsrDI GCAATG 2 cut(s) 236, 716
BsrGI TGTACA 1 cut(s) 761
BsrI ACTGG 2 cut(s) 379, 453
BssECI CCNNGG 1 cut(s) 611
BssMI GATC 4 cut(s) 304, 369, 409, 576
BssNI GRCGYC 2 cut(s) 87, 558
BssT1I CCWWGG 1 cut(s) 611
Bst4CI ACNGT 3 cut(s) 330, 627, 683
Bst6I CTCTTC 3 cut(s) 164, 263, 840
BstACI GRCGYC 2 cut(s) 87, 558
BstAPI GCANNNNNTGC 1 cut(s) 131
BstAUI TGTACA 1 cut(s) 761
BstC8I GCNNGC 2 cut(s) 149, 556
BstDEI CTNAG 4 cut(s) 100, 373, 755, 843
BstF5I GGATG 5 cut(s) 127, 319, 401, 413, 802
BstFNI CGCG 2 cut(s) 63, 119
BstKTI GATC 4 cut(s) 307, 372, 412, 579
BstMAI GTCTC 1 cut(s) 94
BstMBI GATC 4 cut(s) 304, 369, 409, 576
BstMWI GCNNNNNNNGC 4 cut(s) 101, 131, 195, 323
BstNSI RCATGY 1 cut(s) 183
BstSFI CTRYAG 1 cut(s) 549
BstUI CGCG 2 cut(s) 63, 119
BstV1I GCAGC 1 cut(s) 784
BstX2I RGATCY 1 cut(s) 409
BstXI CCANNNNNNTGG 1 cut(s) 736
BstYI RGATCY 1 cut(s) 409
BsuI GTATCC 1 cut(s) 354
BsuRI GGCC 3 cut(s) 301, 665, 689
BtrI CACGTC 1 cut(s) 35
BtsCI GGATG 5 cut(s) 127, 319, 401, 413, 802
BtsIMutI CAGTG 4 cut(s) 326, 386, 417, 446
Cac8I GCNNGC 2 cut(s) 149, 556
CaiI CAGNNNCTG 1 cut(s) 499
CciI TCATGA 1 cut(s) 573
Cfr13I GGNCC 2 cut(s) 300, 663
CseI GACGC 3 cut(s) 13, 485, 547
Csp6I GTAC 1 cut(s) 762
CviAII CATG 5 cut(s) 21, 180, 574, 580, 736
CviQI GTAC 1 cut(s) 762
DdeI CTNAG 4 cut(s) 100, 373, 755, 843
DpnI GATC 4 cut(s) 306, 371, 411, 578
DpnII GATC 4 cut(s) 304, 369, 409, 576
Eam1104I CTCTTC 3 cut(s) 164, 263, 840
EarI CTCTTC 3 cut(s) 164, 263, 840
Eco130I CCWWGG 1 cut(s) 611
EcoRI GAATTC 1 cut(s) 740
EcoT14I CCWWGG 1 cut(s) 611
EcoT22I ATGCAT 2 cut(s) 295, 319
ErhI CCWWGG 1 cut(s) 611
Esp3I CGTCTC 1 cut(s) 94
FaeI CATG 5 cut(s) 24, 183, 577, 583, 739
FalI AAGNNNNNCTT 4 cut(s) 237, 269, 514, 546
FaqI GGGAC 1 cut(s) 43
FatI CATG 5 cut(s) 20, 179, 573, 579, 735
FauI CCCGC 1 cut(s) 547
FauNDI CATATG 1 cut(s) 208
FbaI TGATCA 1 cut(s) 576
Fnu4HI GCNGC 2 cut(s) 528, 773
FokI GGATG 5 cut(s) 134, 326, 400, 408, 809
Fsp4HI GCNGC 2 cut(s) 528, 773
FspBI CTAG 2 cut(s) 153, 612
GluI GCNGC 2 cut(s) 528, 773
GsuI CTGGAG 1 cut(s) 470
HaeIII GGCC 3 cut(s) 301, 665, 689
HapII CCGG 1 cut(s) 105
HgaI GACGC 3 cut(s) 13, 485, 547
Hin1I GRCGYC 2 cut(s) 87, 558
Hin1II CATG 5 cut(s) 24, 183, 577, 583, 739
HincII GTYRAC 1 cut(s) 562
HindII GTYRAC 1 cut(s) 562
HindIII AAGCTT 1 cut(s) 356
HinfI GANTC 4 cut(s) 162, 172, 438, 454
HpaII CCGG 1 cut(s) 105
HphI GGTGA 1 cut(s) 36
Hpy166II GTNNAC 1 cut(s) 562
Hpy188I TCNGA 6 cut(s) 14, 82, 376, 428, 652, 809
Hpy188III TCNNGA 7 cut(s) 28, 166, 311, 347, 362, 481, 574
Hpy8I GTNNAC 1 cut(s) 562
Hpy99I CGWCG 3 cut(s) 29, 86, 89
HpyAV CCTTC 2 cut(s) 580, 834
HpyCH4III ACNGT 3 cut(s) 330, 627, 683
HpyCH4IV ACGT 3 cut(s) 34, 87, 341
HpyCH4V TGCA 6 cut(s) 125, 241, 293, 317, 470, 583
HpyF10VI GCNNNNNNNGC 4 cut(s) 101, 131, 195, 323
HpyF3I CTNAG 4 cut(s) 100, 373, 755, 843
HpySE526I ACGT 3 cut(s) 34, 87, 341
Hsp92I GRCGYC 2 cut(s) 87, 558
Hsp92II CATG 5 cut(s) 24, 183, 577, 583, 739
Ksp22I TGATCA 1 cut(s) 576
Kzo9I GATC 4 cut(s) 304, 369, 409, 576
LmnI GCTCC 1 cut(s) 431
LpnPI CCDG 8 cut(s) 118, 227, 296, 360, 392, 420, 434, 701
Lsp1109I GCAGC 1 cut(s) 784
LweI GCATC 6 cut(s) 112, 121, 280, 302, 304, 611
MaeI CTAG 2 cut(s) 153, 612
MaeII ACGT 3 cut(s) 34, 87, 341
MalI GATC 4 cut(s) 306, 371, 411, 578
MbiI CCGCTC 1 cut(s) 527
MboI GATC 4 cut(s) 304, 369, 409, 576
MboII GAAGA 7 cut(s) 140, 181, 257, 280, 289, 449, 827
MflI RGATCY 1 cut(s) 409
MhlI GDGCHC 1 cut(s) 328
MluCI AATT 4 cut(s) 111, 280, 740, 810
MlyI GAGTC 1 cut(s) 181
MmeI TCCRAC 2 cut(s) 105, 137
MnlI CCTC 8 cut(s) 57, 61, 64, 109, 258, 422, 509, 808
Mph1103I ATGCAT 2 cut(s) 295, 319
MroXI GAANNNNTTC 1 cut(s) 357
MseI TTAA 4 cut(s) 114, 192, 255, 320
MslI CAYNNNNRTG 4 cut(s) 312, 578, 584, 734
MspI CCGG 1 cut(s) 105
MvnI CGCG 2 cut(s) 63, 119
MwoI GCNNNNNNNGC 4 cut(s) 101, 131, 195, 323
NdeI CATATG 1 cut(s) 208
NdeII GATC 4 cut(s) 304, 369, 409, 576
NlaIII CATG 5 cut(s) 24, 183, 577, 583, 739
NsiI ATGCAT 2 cut(s) 295, 319
NspI RCATGY 1 cut(s) 183
PagI TCATGA 1 cut(s) 573
PciI ACATGT 1 cut(s) 179
PdmI GAANNNNTTC 1 cut(s) 357
PfeI GAWTC 3 cut(s) 162, 438, 454
PflFI GACNNNGTC 1 cut(s) 86
PkrI GCNGC 2 cut(s) 529, 774
PleI GAGTC 1 cut(s) 180
PpsI GAGTC 1 cut(s) 180
PscI ACATGT 1 cut(s) 179
PspPI GGNCC 2 cut(s) 300, 663
PsrI GAACNNNNNNTAC 2 cut(s) 716, 748
PstNI CAGNNNCTG 1 cut(s) 499
PsuI RGATCY 1 cut(s) 409
PsyI GACNNNGTC 1 cut(s) 86
RsaI GTAC 1 cut(s) 763
RsaNI GTAC 1 cut(s) 762
RseI CAYNNNNRTG 4 cut(s) 312, 578, 584, 734
SaqAI TTAA 4 cut(s) 114, 192, 255, 320
SatI GCNGC 2 cut(s) 528, 773
Sau3AI GATC 4 cut(s) 304, 369, 409, 576
Sau96I GGNCC 2 cut(s) 300, 663
SchI GAGTC 1 cut(s) 181
SduI GDGCHC 1 cut(s) 328
SfaNI GCATC 6 cut(s) 112, 121, 280, 302, 304, 611
SfcI CTRYAG 1 cut(s) 549
SmiMI CAYNNNNRTG 4 cut(s) 312, 578, 584, 734
SmlI CTYRAG 1 cut(s) 776
SmoI CTYRAG 1 cut(s) 776
Sse9I AATT 4 cut(s) 111, 280, 740, 810
SsiI CCGC 5 cut(s) 63, 119, 196, 527, 554
SspMI CTAG 2 cut(s) 153, 612
StyI CCWWGG 1 cut(s) 611
TaaI ACNGT 3 cut(s) 330, 627, 683
TaiI ACGT 3 cut(s) 37, 90, 344
TaqI TCGA 1 cut(s) 482
TasI AATT 4 cut(s) 111, 280, 740, 810
TatI WGTACW 1 cut(s) 761
TauI GCSGC 1 cut(s) 530
TfiI GAWTC 3 cut(s) 162, 438, 454
Tru1I TTAA 4 cut(s) 114, 192, 255, 320
Tru9I TTAA 4 cut(s) 114, 192, 255, 320
TscAI CASTG 4 cut(s) 333, 386, 424, 453
TseI GCWGC 1 cut(s) 772
TspDTI ATGAA 5 cut(s) 225, 290, 366, 430, 562
TspGWI ACGGA 1 cut(s) 46
TspRI CASTG 4 cut(s) 333, 386, 424, 453
Tth111I GACNNNGTC 1 cut(s) 86
XapI RAATTY 1 cut(s) 740
XceI RCATGY 1 cut(s) 183
XmaJI CCTAGG 1 cut(s) 611
XmnI GAANNNNTTC 1 cut(s) 357
XspI CTAG 2 cut(s) 153, 612
ZraI GACGTC 1 cut(s) 88
Zsp2I ATGCAT 2 cut(s) 295, 319
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.