MD13G1118100.v1.1

ELMO domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0007302
Physical Location & Seq
Forward (+)
8634335 .. 8637864
3530 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1118100.v1.1.491

Sequence Viewer

Length: 837 bp
ATGATGAGAGTTGTTGGGAGTACCAGTAGTCCTCAAACTCTCTCTCCTCCCCCTAATTCCTCCTCATCCTCTACCATCTCCCGCTGCGCTCCTCCCACACACACTTCTGCTTCCATTTCTGCAGATGATGAAACTTGTGGTTCGCCTAAATGGATCGGTAAGGGTCTCACTTGCGTTTGCTTCAAGCGAAAGGGAAATTATCAGCGGATTTGTATGAACTTGACACCCCTTCAGGAAGAAAGAATGGGACGATTGAAGCAAAGAATGAAGGTTTACTTTGATGCTACTAGGCCGGATCACCAGGAAGCGCTGAGAGCTTTATGGTACGCTACATTTCCTGGACAAGAGCTTAAGGGTTTGATATCTGATCAATGGAAAGAGATGGGATGGCAAGGAAGAGATCCGTCTACCGATTTCAGAGGAGCTGGATTCATTTCTTTGGAGAACCTGCTGTTCTTTGCAAAGACATTTTCAGCATCTTTCCAGTGTCTATTGAAAAAGCAGAGAGGAAAGCGAGCTGCTTGGGAATATCCTTTCGCTGTTGCTGGTGTCAACATCACGTTTATGATCATGCAAATGCTTGACCTTGATGCCAGAAAACCCAGGACTTTCATAAGAACAGTTTTCTTACAAATGTTGTTAGAAAATGAATGGACCTTCGACTTGCTGTACTGCGTTGCATTCATGGTTATGGACAAACAGTGGCTCGAGAGAAATGCTACATACATGGAGTTCAATGATGTTTTGAAATCGACGCGAGCTCAACTGGAGAGGGAGCTGCTAATGGATGATGTTTTGCGGATTGAAGACATGCCTTCTTACAGCCTTCTTTGTTGA

Protein Analysis

279

Amino Acids

31.98

Weight (kDa)

8.74

Isoelectric Point (pI)

43.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ELMO_CED12 PF04727 84 - 249 1.8e-52 ELMO/CED-12 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 456
AccI GTMKAC 1 cut(s) 407
AccII CGCG 1 cut(s) 757
AciI CCGC 3 cut(s) 82, 205, 799
AclWI GGATC 3 cut(s) 161, 303, 395
AcuI CTGAAG 1 cut(s) 215
AfaI GTAC 3 cut(s) 22, 326, 671
AfeI AGCGCT 1 cut(s) 309
AflII CTTAAG 1 cut(s) 350
AgsI TTSAA 6 cut(s) 184, 256, 496, 736, 748, 806
AjnI CCWGG 3 cut(s) 300, 337, 602
AluBI AGCT 6 cut(s) 317, 349, 425, 518, 761, 778
AluI AGCT 6 cut(s) 317, 349, 425, 518, 761, 778
Alw21I GWGCWC 1 cut(s) 763
Alw26I GTCTC 1 cut(s) 170
AlwI GGATC 3 cut(s) 161, 303, 395
Ama87I CYCGRG 1 cut(s) 707
Aor51HI AGCGCT 1 cut(s) 309
AoxI GGCC 1 cut(s) 290
ApeKI GCWGC 3 cut(s) 84, 518, 778
AspLEI GCGC 2 cut(s) 89, 310
AspS9I GGNCC 1 cut(s) 654
AsuHPI GGTGA 1 cut(s) 290
AvaI CYCGRG 1 cut(s) 707
AvaII GGWCC 1 cut(s) 654
BanII GRGCYC 1 cut(s) 763
BbsI GAAGAC 1 cut(s) 813
Bbv12I GWGCWC 1 cut(s) 763
BbvI GCAGC 3 cut(s) 71, 505, 765
BccI CCATC 3 cut(s) 83, 376, 381
BcgI CGANNNNNNTGC 2 cut(s) 698, 732
BciT130I CCWGG 3 cut(s) 302, 339, 604
BclI TGATCA 2 cut(s) 367, 567
BcoDI GTCTC 1 cut(s) 170
BfaI CTAG 1 cut(s) 288
BfmI CTRYAG 1 cut(s) 120
BfoI RGCGCY 1 cut(s) 311
BfrI CTTAAG 1 cut(s) 350
BfuAI ACCTGC 1 cut(s) 456
BisI GCNGC 3 cut(s) 85, 519, 779
BlsI GCNGC 3 cut(s) 86, 520, 780
Bme1390I CCNGG 3 cut(s) 302, 339, 604
Bme18I GGWCC 1 cut(s) 654
BmeT110I CYCGRG 1 cut(s) 707
BmgT120I GGNCC 1 cut(s) 654
BmrFI CCNGG 3 cut(s) 302, 339, 604
BmsI GCATC 3 cut(s) 271, 485, 580
BpiI GAAGAC 1 cut(s) 813
BpmI CTGGAG 1 cut(s) 788
BsaI GGTCTC 1 cut(s) 170
BsaJI CCNNGG 1 cut(s) 602
BsaXI ACNNNNNCTCC 4 cut(s) 10, 28, 40, 58
Bse1I ACTGG 3 cut(s) 24, 484, 771
BseBI CCWGG 3 cut(s) 302, 339, 604
BseDI CCNNGG 1 cut(s) 602
BseGI GGATG 3 cut(s) 65, 392, 793
BseMII CTCAG 1 cut(s) 302
BseNI ACTGG 3 cut(s) 24, 484, 771
BseRI GAGGAG 4 cut(s) 36, 52, 81, 435
BseXI GCAGC 3 cut(s) 71, 505, 765
Bsh1236I CGCG 1 cut(s) 757
BshFI GGCC 1 cut(s) 292
BsiHKAI GWGCWC 1 cut(s) 763
BsiHKCI CYCGRG 1 cut(s) 707
BsiSI CCGG 1 cut(s) 293
BslFI GGGAC 1 cut(s) 261
BsmAI GTCTC 1 cut(s) 170
BsmFI GGGAC 1 cut(s) 261
BsmI GAATGC 1 cut(s) 680
BsnI GGCC 1 cut(s) 292
Bso31I GGTCTC 1 cut(s) 170
BsoBI CYCGRG 1 cut(s) 707
Bsp1286I GDGCHC 1 cut(s) 763
Bsp143I GATC 5 cut(s) 153, 295, 367, 400, 567
BspACI CCGC 3 cut(s) 82, 205, 799
BspANI GGCC 1 cut(s) 292
BspCNI CTCAG 1 cut(s) 303
BspFNI CGCG 1 cut(s) 757
BspMAI CTGCAG 1 cut(s) 124
BspMI ACCTGC 1 cut(s) 456
BspPI GGATC 3 cut(s) 161, 303, 395
BspTI CTTAAG 1 cut(s) 350
BspTNI GGTCTC 1 cut(s) 170
BsrI ACTGG 3 cut(s) 24, 484, 771
BssECI CCNNGG 1 cut(s) 602
BssMI GATC 5 cut(s) 153, 295, 367, 400, 567
Bst2UI CCWGG 3 cut(s) 302, 339, 604
Bst4CI ACNGT 2 cut(s) 622, 702
Bst6I CTCTTC 1 cut(s) 391
BstAFI CTTAAG 1 cut(s) 350
BstC8I GCNNGC 2 cut(s) 516, 759
BstDEI CTNAG 1 cut(s) 311
BstF5I GGATG 3 cut(s) 65, 392, 793
BstFNI CGCG 1 cut(s) 757
BstH2I RGCGCY 1 cut(s) 311
BstHHI GCGC 2 cut(s) 89, 310
BstKTI GATC 5 cut(s) 156, 298, 370, 403, 570
BstMAI GTCTC 1 cut(s) 170
BstMBI GATC 5 cut(s) 153, 295, 367, 400, 567
BstMWI GCNNNNNNNGC 1 cut(s) 314
BstNI CCWGG 3 cut(s) 302, 339, 604
BstNSI RCATGY 1 cut(s) 814
BstSCI CCNGG 3 cut(s) 300, 337, 602
BstSFI CTRYAG 1 cut(s) 120
BstUI CGCG 1 cut(s) 757
BstV1I GCAGC 3 cut(s) 71, 505, 765
BstV2I GAAGAC 1 cut(s) 813
BstX2I RGATCY 1 cut(s) 400
BstYI RGATCY 1 cut(s) 400
BsuRI GGCC 1 cut(s) 292
BtsCI GGATG 3 cut(s) 65, 392, 793
BtsIMutI CAGTG 2 cut(s) 491, 707
BveI ACCTGC 1 cut(s) 456
Cac8I GCNNGC 2 cut(s) 516, 759
CfoI GCGC 2 cut(s) 89, 310
Cfr13I GGNCC 1 cut(s) 654
CseI GACGC 1 cut(s) 763
Csp6I GTAC 3 cut(s) 21, 325, 670
CviAII CATG 4 cut(s) 571, 685, 727, 811
CviJI RGCY 9 cut(s) 292, 317, 349, 425, 518, 706, 761, 778, 825
CviKI_1 RGCY 9 cut(s) 292, 317, 349, 425, 518, 706, 761, 778, 825
CviQI GTAC 3 cut(s) 21, 325, 670
DdeI CTNAG 1 cut(s) 311
DpnI GATC 5 cut(s) 155, 297, 369, 402, 569
DpnII GATC 5 cut(s) 153, 295, 367, 400, 567
Eam1104I CTCTTC 1 cut(s) 391
EarI CTCTTC 1 cut(s) 391
Ecl136II GAGCTC 1 cut(s) 761
Eco24I GRGCYC 1 cut(s) 763
Eco31I GGTCTC 1 cut(s) 170
Eco32I GATATC 1 cut(s) 363
Eco47I GGWCC 1 cut(s) 654
Eco47III AGCGCT 1 cut(s) 309
Eco53kI GAGCTC 1 cut(s) 761
Eco57I CTGAAG 1 cut(s) 215
Eco88I CYCGRG 1 cut(s) 707
EcoICRI GAGCTC 1 cut(s) 761
EcoRII CCWGG 3 cut(s) 300, 337, 602
EcoRV GATATC 1 cut(s) 363
EcoT38I GRGCYC 1 cut(s) 763
FaeI CATG 4 cut(s) 574, 688, 730, 814
FalI AAGNNNNNCTT 2 cut(s) 260, 292
FaqI GGGAC 1 cut(s) 261
FatI CATG 4 cut(s) 570, 684, 726, 810
FauI CCCGC 1 cut(s) 89
FbaI TGATCA 2 cut(s) 367, 567
FblI GTMKAC 1 cut(s) 407
Fnu4HI GCNGC 3 cut(s) 85, 519, 779
FokI GGATG 3 cut(s) 52, 399, 800
FriOI GRGCYC 1 cut(s) 763
Fsp4HI GCNGC 3 cut(s) 85, 519, 779
FspBI CTAG 1 cut(s) 288
GlaI GCGC 2 cut(s) 88, 309
GluI GCNGC 3 cut(s) 85, 519, 779
GsuI CTGGAG 1 cut(s) 788
HaeII RGCGCY 1 cut(s) 311
HaeIII GGCC 1 cut(s) 292
HapII CCGG 1 cut(s) 293
HgaI GACGC 1 cut(s) 763
HhaI GCGC 2 cut(s) 89, 310
Hin1II CATG 4 cut(s) 574, 688, 730, 814
Hin6I GCGC 2 cut(s) 87, 308
HinP1I GCGC 2 cut(s) 87, 308
HincII GTYRAC 1 cut(s) 553
HindII GTYRAC 1 cut(s) 553
HinfI GANTC 1 cut(s) 429
HpaII CCGG 1 cut(s) 293
HphI GGTGA 1 cut(s) 290
Hpy166II GTNNAC 3 cut(s) 274, 408, 553
Hpy188I TCNGA 2 cut(s) 367, 419
Hpy188III TCNNGA 2 cut(s) 233, 709
Hpy8I GTNNAC 3 cut(s) 274, 408, 553
Hpy99I CGWCG 1 cut(s) 757
HpyAV CCTTC 5 cut(s) 239, 262, 667, 825, 836
HpyCH4III ACNGT 2 cut(s) 622, 702
HpyCH4IV ACGT 1 cut(s) 560
HpyCH4V TGCA 4 cut(s) 122, 461, 574, 680
HpyF10VI GCNNNNNNNGC 1 cut(s) 314
HpyF3I CTNAG 1 cut(s) 311
HpySE526I ACGT 1 cut(s) 560
Hsp92II CATG 4 cut(s) 574, 688, 730, 814
HspAI GCGC 2 cut(s) 87, 308
Ksp22I TGATCA 2 cut(s) 367, 567
Kzo9I GATC 5 cut(s) 153, 295, 367, 400, 567
LmnI GCTCC 3 cut(s) 94, 422, 775
Lsp1109I GCAGC 3 cut(s) 71, 505, 765
LweI GCATC 3 cut(s) 271, 485, 580
MaeI CTAG 1 cut(s) 288
MaeII ACGT 1 cut(s) 560
MalI GATC 5 cut(s) 155, 297, 369, 402, 569
MboI GATC 5 cut(s) 153, 295, 367, 400, 567
MboII GAAGA 3 cut(s) 248, 408, 818
MflI RGATCY 1 cut(s) 400
MhlI GDGCHC 1 cut(s) 763
MluCI AATT 2 cut(s) 55, 196
MnlI CCTC 9 cut(s) 42, 57, 70, 73, 79, 102, 413, 500, 765
MseI TTAA 1 cut(s) 351
MslI CAYNNNNRTG 3 cut(s) 563, 575, 689
MspA1I CMGCKG 2 cut(s) 84, 205
MspCI CTTAAG 1 cut(s) 350
MspI CCGG 1 cut(s) 293
MspR9I CCNGG 3 cut(s) 302, 339, 604
Mva1269I GAATGC 1 cut(s) 680
MvaI CCWGG 3 cut(s) 302, 339, 604
MvnI CGCG 1 cut(s) 757
MwoI GCNNNNNNNGC 1 cut(s) 314
NdeII GATC 5 cut(s) 153, 295, 367, 400, 567
NlaIII CATG 4 cut(s) 574, 688, 730, 814
NspI RCATGY 1 cut(s) 814
PaeR7I CTCGAG 1 cut(s) 707
PctI GAATGC 1 cut(s) 680
PfeI GAWTC 1 cut(s) 429
PfoI TCCNGGA 1 cut(s) 337
PkrI GCNGC 3 cut(s) 86, 520, 780
Psp124BI GAGCTC 1 cut(s) 763
Psp6I CCWGG 3 cut(s) 300, 337, 602
PspGI CCWGG 3 cut(s) 300, 337, 602
PspPI GGNCC 1 cut(s) 654
PstI CTGCAG 1 cut(s) 124
PsuI RGATCY 1 cut(s) 400
RsaI GTAC 3 cut(s) 22, 326, 671
RsaNI GTAC 3 cut(s) 21, 325, 670
RseI CAYNNNNRTG 3 cut(s) 563, 575, 689
SacI GAGCTC 1 cut(s) 763
SaqAI TTAA 1 cut(s) 351
SatI GCNGC 3 cut(s) 85, 519, 779
Sau3AI GATC 5 cut(s) 153, 295, 367, 400, 567
Sau96I GGNCC 1 cut(s) 654
ScrFI CCNGG 3 cut(s) 302, 339, 604
SduI GDGCHC 1 cut(s) 763
SfaNI GCATC 3 cut(s) 271, 485, 580
SfcI CTRYAG 1 cut(s) 120
Sfr274I CTCGAG 1 cut(s) 707
SinI GGWCC 1 cut(s) 654
SlaI CTCGAG 1 cut(s) 707
SmiMI CAYNNNNRTG 3 cut(s) 563, 575, 689
SmlI CTYRAG 2 cut(s) 350, 707
SmoI CTYRAG 2 cut(s) 350, 707
Sse9I AATT 2 cut(s) 55, 196
SsiI CCGC 3 cut(s) 82, 205, 799
SspMI CTAG 1 cut(s) 288
SstI GAGCTC 1 cut(s) 763
StyD4I CCNGG 3 cut(s) 300, 337, 602
TaaI ACNGT 2 cut(s) 622, 702
TaiI ACGT 1 cut(s) 563
TaqI TCGA 3 cut(s) 660, 708, 752
TasI AATT 2 cut(s) 55, 196
TatI WGTACW 1 cut(s) 669
TfiI GAWTC 1 cut(s) 429
Tru1I TTAA 1 cut(s) 351
Tru9I TTAA 1 cut(s) 351
TscAI CASTG 2 cut(s) 491, 707
TseI GCWGC 3 cut(s) 84, 518, 778
TspDTI ATGAA 7 cut(s) 144, 230, 281, 421, 601, 663, 673
TspGWI ACGGA 1 cut(s) 393
TspRI CASTG 2 cut(s) 491, 707
Vha464I CTTAAG 1 cut(s) 350
VpaK11BI GGWCC 1 cut(s) 654
XceI RCATGY 1 cut(s) 814
XhoI CTCGAG 1 cut(s) 707
XmiI GTMKAC 1 cut(s) 407
XspI CTAG 1 cut(s) 288
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.