AT1G72340

Belongs to the eIF-2B alpha beta delta subunits family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0006573
Physical Location & Seq
Forward (+)
27236797 .. 27238720
1924 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G72340.1

Sequence Viewer

Length: 1149 bp
ATGTGGAGAAGATCACCGTCGTTTATTCTCGACGAGCGACGAAGCTCGAATTCACCTCCAATGGCGGATACAACACGAGGTCCTTTTCAAAACCCTGATTCCATCTCCGCTTATTACCAAACTCGCGCGGCGCATCACGGTGTCATTACAAGCGACTGGCTAGCCCAAGCTCAAGCCGCCGTTGGCGGCGTCTCAGGCGATGCCCAGCATGACTTATCCGTAACTGATCTCGGGAACGAGAAGTCGTTTAATGTAATTGAAGAGTTCAATAACTGGAGAAAACAACCGGATCTGGCTGAAGCCGTCGCGGCTATACGAGCTTTGGCTGCTGTTATTCGTGCGAGCGAAGCGAGCACCATGATGGAGCTTGAAATTGAGCTCAAAAAGGCTTCTGATACACTCAAGTCGTGGGACAAAACGTCGATCTCTTTGACAGCTGGATGCGATTTGTTTATCAGATACGTTACGAGAACGTCTGCTTTGGAATATGAAGATTTCAATTCAGCTAAGTCTCGTCTTCTTGAACGTGCTGAGAAATTTGGGGAAATATCTTGCAAGGCTCGTAGGATTATCGCAATGCTTAGTCAAGATTTTATATTCGATGGTTGTACCATATTGGTTCATGGACTCTCTAGAGTTGTTCTGGAAATACTTAAGACTGCTGCACAAAACAACAAGCTCTTTCGAGTTCTCTGCACAGAGGGGAGGCCGGATGGAACAGGAGTTCTGTTATCGAGTGAGCTATCGAAGCTCGATATCCCAGTGAAACTACTGCTTGATTCAGCTGTGGCGTATAGTATGGACGAGGTAGACATGGTGTTTGTCGGGGCGGATGGAGTTGTAGAAAGTGGCGGTATTATCAACATGATGGGAACATACCAAATCGCACTTGTGGCACACAGCATGAACAAACCGGTCTATGTTGCTGCTGAGAGTTACAAGTTCGCTAGGCTTTACCCATTAGACCAAAAGGACATGGCACCGGCGTTGAGACCAATCGAATTTGGTGTAAAGATTCCGACAAAGGTGGAAGTAGAGAGGTCAGCTCGAGACTATACTCCGCCTCAGTACCTAACACTCCTCTTCACTGACCTTGGTGTTCTCTCTCCCTCTGTAGTTAGCGATGAGCTTATTCAACTCTATCTCTAA

Protein Analysis

382

Amino Acids

42.19

Weight (kDa)

5.25

Isoelectric Point (pI)

39.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
IF-2B PF01008 95 - 370 1.2e-72 Initiation factor 2 subunit family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 979
AccI GTMKAC 1 cut(s) 810
AccII CGCG 3 cut(s) 126, 128, 308
AciI CCGC 9 cut(s) 65, 108, 128, 177, 186, 308, 830, 852, 1061
AclWI GGATC 1 cut(s) 297
AcsI RAATTY 3 cut(s) 49, 536, 1001
AcuI CTGAAG 1 cut(s) 318
AcyI GRCGYC 1 cut(s) 189
AfaI GTAC 2 cut(s) 610, 1070
AflII CTTAAG 1 cut(s) 653
AgeI ACCGGT 1 cut(s) 913
AgsI TTSAA 7 cut(s) 89, 260, 268, 371, 499, 524, 1136
AhdI GACNNNNNGTC 1 cut(s) 418
AloI GAACNNNNNNTCC 2 cut(s) 708, 740
Alw21I GWGCWC 2 cut(s) 356, 381
Alw26I GTCTC 4 cut(s) 196, 516, 985, 1044
AlwI GGATC 1 cut(s) 297
Ama87I CYCGRG 2 cut(s) 230, 1047
AoxI GGCC 1 cut(s) 707
ApeKI GCWGC 3 cut(s) 326, 662, 926
ApoI RAATTY 3 cut(s) 49, 536, 1001
ArsI GACNNNNNNTTYG 2 cut(s) 803, 835
AsiGI ACCGGT 1 cut(s) 913
AspLEI GCGC 2 cut(s) 128, 133
AspS9I GGNCC 1 cut(s) 80
AsuHPI GGTGA 2 cut(s) 6, 45
AsuNHI GCTAGC 1 cut(s) 160
AvaI CYCGRG 2 cut(s) 230, 1047
AvaII GGWCC 1 cut(s) 80
BanI GGYRCC 1 cut(s) 979
BanII GRGCYC 1 cut(s) 381
BauI CACGAG 1 cut(s) 75
BbsI GAAGAC 1 cut(s) 509
Bbv12I GWGCWC 2 cut(s) 356, 381
BbvI GCAGC 3 cut(s) 313, 649, 913
BccI CCATC 6 cut(s) 110, 355, 596, 707, 827, 862
BceAI ACGGC 2 cut(s) 164, 287
BcgI CGANNNNNNTGC 2 cut(s) 675, 709
BciVI GTATCC 1 cut(s) 61
BcoDI GTCTC 4 cut(s) 196, 516, 985, 1044
BfaI CTAG 3 cut(s) 161, 633, 948
BfmI CTRYAG 1 cut(s) 1113
BfrI CTTAAG 1 cut(s) 653
BfuI GTATCC 1 cut(s) 61
BglI GCCNNNNNGGC 1 cut(s) 308
BisI GCNGC 7 cut(s) 129, 177, 187, 309, 327, 663, 927
BlsI GCNGC 7 cut(s) 130, 178, 188, 310, 328, 664, 928
Bme18I GGWCC 1 cut(s) 80
BmeRI GACNNNNNGTC 1 cut(s) 418
BmeT110I CYCGRG 2 cut(s) 230, 1047
BmgT120I GGNCC 1 cut(s) 80
BmiI GGNNCC 1 cut(s) 981
BmrI ACTGGG 1 cut(s) 755
BmsI GCATC 3 cut(s) 142, 190, 431
BmtI GCTAGC 1 cut(s) 164
BmuI ACTGGG 1 cut(s) 755
BpiI GAAGAC 1 cut(s) 509
BplI GAGNNNNNCTC 2 cut(s) 1030, 1062
BpmI CTGGAG 1 cut(s) 295
BpuEI CTTGAG 2 cut(s) 156, 386
BsaHI GRCGYC 1 cut(s) 189
BsaI GGTCTC 1 cut(s) 985
BsaJI CCNNGG 1 cut(s) 1093
BsaWI WCCGGW 2 cut(s) 286, 913
Bse118I RCCGGY 2 cut(s) 913, 982
Bse1I ACTGG 3 cut(s) 161, 278, 761
Bse3DI GCAATG 1 cut(s) 582
BseDI CCNNGG 1 cut(s) 1093
BseGI GGATG 3 cut(s) 446, 718, 838
BseMI GCAATG 1 cut(s) 582
BseMII CTCAG 4 cut(s) 207, 522, 921, 1079
BseNI ACTGG 3 cut(s) 161, 278, 761
BseRI GAGGAG 1 cut(s) 1070
BseXI GCAGC 3 cut(s) 313, 649, 913
BseYI CCCAGC 1 cut(s) 204
BsgI GTGCAG 2 cut(s) 648, 679
Bsh1236I CGCG 3 cut(s) 126, 128, 308
BshFI GGCC 1 cut(s) 709
BshNI GGYRCC 1 cut(s) 979
BshTI ACCGGT 1 cut(s) 913
BsiHKAI GWGCWC 2 cut(s) 356, 381
BsiHKCI CYCGRG 2 cut(s) 230, 1047
BsiSI CCGG 4 cut(s) 287, 710, 914, 983
BslFI GGGAC 1 cut(s) 425
BsmAI GTCTC 4 cut(s) 196, 516, 985, 1044
BsmBI CGTCTC 1 cut(s) 196
BsmFI GGGAC 1 cut(s) 425
BsnI GGCC 1 cut(s) 709
Bso31I GGTCTC 1 cut(s) 985
BsoBI CYCGRG 2 cut(s) 230, 1047
Bsp1286I GDGCHC 2 cut(s) 356, 381
Bsp143I GATC 4 cut(s) 11, 226, 289, 423
BspACI CCGC 9 cut(s) 65, 108, 128, 177, 186, 308, 830, 852, 1061
BspANI GGCC 1 cut(s) 709
BspCNI CTCAG 4 cut(s) 206, 523, 922, 1078
BspFNI CGCG 3 cut(s) 126, 128, 308
BspLI GGNNCC 1 cut(s) 981
BspOI GCTAGC 1 cut(s) 164
BspPI GGATC 1 cut(s) 297
BspT107I GGYRCC 1 cut(s) 979
BspTI CTTAAG 1 cut(s) 653
BspTNI GGTCTC 1 cut(s) 985
BsrDI GCAATG 1 cut(s) 582
BsrFI RCCGGY 2 cut(s) 913, 982
BsrI ACTGG 3 cut(s) 161, 278, 761
BssAI RCCGGY 2 cut(s) 913, 982
BssECI CCNNGG 1 cut(s) 1093
BssMI GATC 4 cut(s) 11, 226, 289, 423
BssNI GRCGYC 1 cut(s) 189
BssSI CACGAG 1 cut(s) 75
BssT1I CCWWGG 1 cut(s) 1093
Bst2BI CACGAG 1 cut(s) 75
Bst4CI ACNGT 2 cut(s) 18, 140
Bst6I CTCTTC 2 cut(s) 255, 1088
BstACI GRCGYC 1 cut(s) 189
BstAFI CTTAAG 1 cut(s) 653
BstC8I GCNNGC 3 cut(s) 162, 343, 352
BstDEI CTNAG 6 cut(s) 193, 507, 531, 581, 930, 1065
BstF5I GGATG 3 cut(s) 446, 718, 838
BstFNI CGCG 3 cut(s) 126, 128, 308
BstHHI GCGC 2 cut(s) 128, 133
BstKTI GATC 4 cut(s) 14, 229, 292, 426
BstMAI GTCTC 4 cut(s) 196, 516, 985, 1044
BstMBI GATC 4 cut(s) 11, 226, 289, 423
BstMWI GCNNNNNNNGC 9 cut(s) 176, 195, 308, 317, 326, 347, 351, 748, 893
BstSFI CTRYAG 1 cut(s) 1113
BstUI CGCG 3 cut(s) 126, 128, 308
BstV1I GCAGC 3 cut(s) 313, 649, 913
BstV2I GAAGAC 1 cut(s) 509
BstX2I RGATCY 1 cut(s) 289
BstYI RGATCY 1 cut(s) 289
BsuI GTATCC 1 cut(s) 61
BsuRI GGCC 1 cut(s) 709
BtgZI GCGATG 2 cut(s) 213, 1137
BtsCI GGATG 3 cut(s) 446, 718, 838
BtsIMutI CAGTG 2 cut(s) 768, 1086
Cac8I GCNNGC 3 cut(s) 162, 343, 352
CfoI GCGC 2 cut(s) 128, 133
Cfr10I RCCGGY 2 cut(s) 913, 982
Cfr13I GGNCC 1 cut(s) 80
CseI GACGC 1 cut(s) 178
Csp6I GTAC 2 cut(s) 609, 1069
CspAI ACCGGT 1 cut(s) 913
CviAII CATG 7 cut(s) 209, 358, 623, 814, 865, 904, 976
CviQI GTAC 2 cut(s) 609, 1069
DdeI CTNAG 6 cut(s) 193, 507, 531, 581, 930, 1065
DpnI GATC 4 cut(s) 13, 228, 291, 425
DpnII GATC 4 cut(s) 11, 226, 289, 423
DriI GACNNNNNGTC 1 cut(s) 418
Eam1104I CTCTTC 2 cut(s) 255, 1088
Eam1105I GACNNNNNGTC 1 cut(s) 418
EarI CTCTTC 2 cut(s) 255, 1088
EciI GGCGGA 3 cut(s) 80, 845, 1050
Ecl136II GAGCTC 1 cut(s) 379
Eco130I CCWWGG 1 cut(s) 1093
Eco24I GRGCYC 1 cut(s) 381
Eco31I GGTCTC 1 cut(s) 985
Eco32I GATATC 1 cut(s) 757
Eco47I GGWCC 1 cut(s) 80
Eco53kI GAGCTC 1 cut(s) 379
Eco57I CTGAAG 1 cut(s) 318
Eco88I CYCGRG 2 cut(s) 230, 1047
EcoICRI GAGCTC 1 cut(s) 379
EcoO109I RGGNCCY 1 cut(s) 80
EcoRI GAATTC 1 cut(s) 49
EcoRV GATATC 1 cut(s) 757
EcoT14I CCWWGG 1 cut(s) 1093
EcoT38I GRGCYC 1 cut(s) 381
ErhI CCWWGG 1 cut(s) 1093
Esp3I CGTCTC 1 cut(s) 196
FaeI CATG 7 cut(s) 212, 361, 626, 817, 868, 907, 979
FaqI GGGAC 1 cut(s) 425
FatI CATG 7 cut(s) 208, 357, 622, 813, 864, 903, 975
FblI GTMKAC 1 cut(s) 810
Fnu4HI GCNGC 7 cut(s) 129, 177, 187, 309, 327, 663, 927
FokI GGATG 3 cut(s) 453, 725, 845
FriOI GRGCYC 1 cut(s) 381
Fsp4HI GCNGC 7 cut(s) 129, 177, 187, 309, 327, 663, 927
FspBI CTAG 3 cut(s) 161, 633, 948
GlaI GCGC 2 cut(s) 127, 132
GluI GCNGC 7 cut(s) 129, 177, 187, 309, 327, 663, 927
GsaI CCCAGC 1 cut(s) 208
GsuI CTGGAG 1 cut(s) 295
HaeIII GGCC 1 cut(s) 709
HapII CCGG 4 cut(s) 287, 710, 914, 983
HgaI GACGC 1 cut(s) 178
HhaI GCGC 2 cut(s) 128, 133
Hin1I GRCGYC 1 cut(s) 189
Hin1II CATG 7 cut(s) 212, 361, 626, 817, 868, 907, 979
Hin6I GCGC 2 cut(s) 126, 131
HinP1I GCGC 2 cut(s) 126, 131
HinfI GANTC 4 cut(s) 98, 627, 779, 1015
HpaII CCGG 4 cut(s) 287, 710, 914, 983
HphI GGTGA 2 cut(s) 6, 45
Hpy166II GTNNAC 1 cut(s) 811
Hpy188I TCNGA 3 cut(s) 394, 458, 1020
Hpy188III TCNNGA 7 cut(s) 29, 232, 521, 587, 633, 644, 1049
Hpy8I GTNNAC 1 cut(s) 811
Hpy99I CGWCG 5 cut(s) 22, 35, 42, 308, 424
HpyCH4III ACNGT 2 cut(s) 18, 140
HpyCH4IV ACGT 4 cut(s) 419, 462, 473, 526
HpyCH4V TGCA 3 cut(s) 555, 665, 696
HpyF10VI GCNNNNNNNGC 9 cut(s) 176, 195, 308, 317, 326, 347, 351, 748, 893
HpyF3I CTNAG 6 cut(s) 193, 507, 531, 581, 930, 1065
HpySE526I ACGT 4 cut(s) 419, 462, 473, 526
Hsp92I GRCGYC 1 cut(s) 189
Hsp92II CATG 7 cut(s) 212, 361, 626, 817, 868, 907, 979
HspAI GCGC 2 cut(s) 126, 131
Kzo9I GATC 4 cut(s) 11, 226, 289, 423
LmnI GCTCC 1 cut(s) 364
Lsp1109I GCAGC 3 cut(s) 313, 649, 913
LweI GCATC 3 cut(s) 142, 190, 431
MaeI CTAG 3 cut(s) 161, 633, 948
MaeII ACGT 4 cut(s) 419, 462, 473, 526
MaeIII GTNAC 3 cut(s) 220, 463, 935
MalI GATC 4 cut(s) 13, 228, 291, 425
MboI GATC 4 cut(s) 11, 226, 289, 423
MboII GAAGA 5 cut(s) 21, 272, 503, 509, 1075
MflI RGATCY 1 cut(s) 289
MhlI GDGCHC 2 cut(s) 356, 381
MluCI AATT 6 cut(s) 49, 255, 372, 499, 536, 1001
MlyI GAGTC 1 cut(s) 621
MmeI TCCRAC 1 cut(s) 1043
MnlI CCTC 9 cut(s) 66, 71, 694, 699, 799, 1032, 1074, 1091, 1120
MseI TTAA 2 cut(s) 249, 654
MslI CAYNNNNRTG 2 cut(s) 138, 359
MspA1I CMGCKG 2 cut(s) 437, 785
MspCI CTTAAG 1 cut(s) 653
MspI CCGG 4 cut(s) 287, 710, 914, 983
MteI GCGCNGCGC 1 cut(s) 129
MvnI CGCG 3 cut(s) 126, 128, 308
MwoI GCNNNNNNNGC 9 cut(s) 176, 195, 308, 317, 326, 347, 351, 748, 893
NdeII GATC 4 cut(s) 11, 226, 289, 423
NheI GCTAGC 1 cut(s) 160
NlaIII CATG 7 cut(s) 212, 361, 626, 817, 868, 907, 979
NlaIV GGNNCC 1 cut(s) 981
PaeR7I CTCGAG 1 cut(s) 1047
PfeI GAWTC 3 cut(s) 98, 779, 1015
PinAI ACCGGT 1 cut(s) 913
PkrI GCNGC 7 cut(s) 130, 178, 188, 310, 328, 664, 928
PleI GAGTC 1 cut(s) 621
PpsI GAGTC 1 cut(s) 621
PpuMI RGGWCCY 1 cut(s) 80
Psp124BI GAGCTC 1 cut(s) 381
Psp5II RGGWCCY 1 cut(s) 80
PspFI CCCAGC 1 cut(s) 204
PspN4I GGNNCC 1 cut(s) 981
PspPI GGNCC 1 cut(s) 80
PspPPI RGGWCCY 1 cut(s) 80
PsuI RGATCY 1 cut(s) 289
PvuII CAGCTG 2 cut(s) 437, 785
RsaI GTAC 2 cut(s) 610, 1070
RsaNI GTAC 2 cut(s) 609, 1069
RseI CAYNNNNRTG 2 cut(s) 138, 359
SacI GAGCTC 1 cut(s) 381
SaqAI TTAA 2 cut(s) 249, 654
SatI GCNGC 7 cut(s) 129, 177, 187, 309, 327, 663, 927
Sau3AI GATC 4 cut(s) 11, 226, 289, 423
Sau96I GGNCC 1 cut(s) 80
SchI GAGTC 1 cut(s) 621
SduI GDGCHC 2 cut(s) 356, 381
SfaNI GCATC 3 cut(s) 142, 190, 431
SfcI CTRYAG 1 cut(s) 1113
Sfr274I CTCGAG 1 cut(s) 1047
SgrAI CRCCGGYG 1 cut(s) 982
SinI GGWCC 1 cut(s) 80
SlaI CTCGAG 1 cut(s) 1047
SmiMI CAYNNNNRTG 2 cut(s) 138, 359
SmlI CTYRAG 4 cut(s) 171, 401, 653, 1047
SmoI CTYRAG 4 cut(s) 171, 401, 653, 1047
Sse9I AATT 6 cut(s) 49, 255, 372, 499, 536, 1001
SsiI CCGC 9 cut(s) 65, 108, 128, 177, 186, 308, 830, 852, 1061
SspMI CTAG 3 cut(s) 161, 633, 948
SstI GAGCTC 1 cut(s) 381
StyI CCWWGG 1 cut(s) 1093
TaaI ACNGT 2 cut(s) 18, 140
TaiI ACGT 4 cut(s) 422, 465, 476, 529
TasI AATT 6 cut(s) 49, 255, 372, 499, 536, 1001
TauI GCSGC 4 cut(s) 131, 179, 189, 311
TfiI GAWTC 3 cut(s) 98, 779, 1015
Tru1I TTAA 2 cut(s) 249, 654
Tru9I TTAA 2 cut(s) 249, 654
TscAI CASTG 2 cut(s) 768, 1093
TseI GCWGC 3 cut(s) 326, 662, 926
TspDTI ATGAA 3 cut(s) 504, 611, 920
TspGWI ACGGA 1 cut(s) 208
TspRI CASTG 2 cut(s) 768, 1093
Vha464I CTTAAG 1 cut(s) 653
VpaK11BI GGWCC 1 cut(s) 80
XapI RAATTY 3 cut(s) 49, 536, 1001
XbaI TCTAGA 1 cut(s) 632
XhoI CTCGAG 1 cut(s) 1047
XmiI GTMKAC 1 cut(s) 810
XspI CTAG 3 cut(s) 161, 633, 948
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.