FvH4_6g35750

Belongs to the eIF-2B alpha beta delta subunits family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0006573
Physical Location & Seq
Reverse (-)
28196585 .. 28200209
3625 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g35750.t4

Sequence Viewer

Length: 1209 bp
ATGTGGTGGAGATCCGCCTCCTTCATTCTCGACAATCGACCCCAAAACGACGCCGCACCGCCTCCCAGACCCGACTCCCTCACTCTCTCCCCTCCCCACCACTCCTCCACCACCATGGCCGACGCCGCCCTCCCAAACCCTAACGCCAACTCCGCCTACTACCAGGCTCGCGCCGCCCACACCGGCGTCGTCACCAGCGACTGGCTCGCCCAGGCCCAGGCCGCCGTCGGCCGCAACCCCGACGACCAGGCCTCCGTCGTCGCCTCCGAGGCTGAGACCAAGGCGGCGTCGGAGTCCGGGAAGCCGTTCAGCGTTATCGAGGAGTTTAATAGCTGGCGGAAGCAGCCGGATTTGGCTGAGGCCGTGGCGGCGATTCGGGCCTTGGCGTCTGTTATTAGGTCTAGCGAGGCTACTACTATGATGGAGCTCGAGATTGAGCTCAAGAAGGCTTCCGATTCTCTTAAGTCATGGGATACAACATCTATATCTTTGACTGCTGGATGCGACCTGTTCATGCGATATGTTACTCGAACTTCAGCTCTGGAGTATGAGGACTTCAACTCAGCCAAGTCTCGTTTGATTGAACGTGCAGAGAAGTTCGGAGAAATATCCACAAAGGCTCGCAGAATCATTGCTGTCCTTAGTCAAGACTTTATATTTGATGGTTGCACCATCTTGGTTCATGGTTTCTCTAGAGTCGTTCTAGAAGTACTGAAGACCGCGGCACAGAGCAAAAAGCTCTTTCGAGTTTTCTGCACAGAGGGAAGACCAGACAGAACAGGATTACGTTTATCAAACGAGCTGACCAAGCTTGATGTTCCTGTGAAGCTCCTAATAGACTCTGCAGTGGCATATACCATGGATGAGGTTGACATGGTGTTTGTTGGGGCAGATGGAGTGGTTGAAAGTGGAGGCATTATAAATATGATGGGTACATATCAAATCGCACTGGTGGCACATAGCATGAACAAACCAGTTTATGTGGCTGCTGAAAGCTACAAGTTTGCTCGCCTTTATCCATTGGATCAGAAAGATTTGGCCCCTGCATTACGCCCCATTGATTTTGGAGTTCCCATCCCGTCCAAGGTGGAAGTTGAAAGATCTGCCCGTGATTATACGCCTCCTCAGTATCTCACTCTACTCTTCACAGACTTGGGTGTGCTCACCCCATCGGTTGTCAGTGATGAGCTGATTCAACTGTACTTGTAA

Protein Analysis

403

Amino Acids

44.15

Weight (kDa)

5.25

Isoelectric Point (pI)

38.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
IF-2B PF01008 115 - 253 1.5e-23 Initiation factor 2 subunit family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 920
AccB7I CCANNNNNTGG 1 cut(s) 201
AccII CGCG 2 cut(s) 171, 722
AclWI GGATC 2 cut(s) 6, 1032
AcoI YGGCCR 2 cut(s) 117, 229
AcuI CTGAAG 2 cut(s) 519, 734
AcyI GRCGYC 5 cut(s) 51, 123, 186, 287, 386
AfaI GTAC 3 cut(s) 711, 934, 1202
AfiI CCNNNNNNNGG 3 cut(s) 201, 217, 1084
AflII CTTAAG 1 cut(s) 461
AgsI TTSAA 5 cut(s) 559, 584, 905, 1097, 1196
AjnI CCWGG 4 cut(s) 162, 210, 216, 246
Alw21I GWGCWC 3 cut(s) 429, 441, 1164
Alw26I GTCTC 2 cut(s) 269, 576
AlwI GGATC 2 cut(s) 6, 1032
AlwNI CAGNNNCTG 1 cut(s) 201
Ama87I CYCGRG 1 cut(s) 428
AoxI GGCC 8 cut(s) 117, 213, 219, 229, 249, 360, 378, 1038
ApeKI GCWGC 2 cut(s) 343, 986
ArsI GACNNNNNNTTYG 4 cut(s) 641, 673, 863, 895
Asp700I GAANNNNTTC 1 cut(s) 305
AspLEI GCGC 1 cut(s) 173
AspS9I GGNCC 3 cut(s) 214, 378, 1039
AsuC2I CCSGG 1 cut(s) 298
AsuHPI GGTGA 2 cut(s) 184, 1156
AvaI CYCGRG 1 cut(s) 428
BanII GRGCYC 2 cut(s) 429, 441
BarI GAAGNNNNNNTAC 2 cut(s) 539, 571
BbsI GAAGAC 2 cut(s) 722, 772
Bbv12I GWGCWC 3 cut(s) 429, 441, 1164
BbvCI CCTCAGC 1 cut(s) 357
BbvI GCAGC 2 cut(s) 355, 973
BccI CCATC 7 cut(s) 415, 656, 680, 887, 922, 1082, 1177
BceAI ACGGC 3 cut(s) 209, 289, 347
BcgI CGANNNNNNTGC 2 cut(s) 735, 769
BciT130I CCWGG 4 cut(s) 164, 212, 218, 248
BciVI GTATCC 1 cut(s) 466
BcnI CCSGG 1 cut(s) 298
BcoDI GTCTC 2 cut(s) 269, 576
BfaI CTAG 3 cut(s) 402, 693, 704
BfmI CTRYAG 1 cut(s) 843
BfrI CTTAAG 1 cut(s) 461
BfuI GTATCC 1 cut(s) 466
BglI GCCNNNNNGGC 2 cut(s) 269, 368
BglII AGATCT 1 cut(s) 1100
BmcAI AGTACT 1 cut(s) 711
Bme1390I CCNGG 5 cut(s) 164, 212, 218, 248, 298
BmeT110I CYCGRG 1 cut(s) 428
BmgT120I GGNCC 3 cut(s) 214, 378, 1039
BmiI GGNNCC 1 cut(s) 1041
BmrFI CCNGG 5 cut(s) 164, 212, 218, 248, 298
BmsI GCATC 1 cut(s) 491
BpiI GAAGAC 2 cut(s) 722, 772
BpmI CTGGAG 1 cut(s) 563
Bpu10I CCTNAGC 1 cut(s) 357
BpuEI CTTGAG 1 cut(s) 425
BpuMI CCSGG 1 cut(s) 298
BsaHI GRCGYC 5 cut(s) 51, 123, 186, 287, 386
BsaI GGTCTC 1 cut(s) 269
BsaXI ACNNNNNCTCC 6 cut(s) 89, 119, 134, 164, 236, 266
Bsc4I CCNNNNNNNGG 3 cut(s) 201, 217, 1084
Bse118I RCCGGY 1 cut(s) 182
Bse1I ACTGG 3 cut(s) 206, 954, 974
Bse3DI GCAATG 1 cut(s) 630
BseBI CCWGG 4 cut(s) 164, 212, 218, 248
BseGI GGATG 3 cut(s) 506, 868, 1074
BseLI CCNNNNNNNGG 3 cut(s) 201, 217, 1084
BseMI GCAATG 1 cut(s) 630
BseMII CTCAG 4 cut(s) 264, 348, 576, 1139
BseNI ACTGG 3 cut(s) 206, 954, 974
BseRI GAGGAG 3 cut(s) 94, 335, 1113
BseX3I CGGCCG 1 cut(s) 229
BseXI GCAGC 2 cut(s) 355, 973
BsgI GTGCAG 2 cut(s) 609, 739
Bsh1236I CGCG 2 cut(s) 171, 722
Bsh1285I CGRYCG 1 cut(s) 232
BshFI GGCC 8 cut(s) 119, 215, 221, 231, 251, 362, 380, 1040
BsiEI CGRYCG 1 cut(s) 232
BsiHKAI GWGCWC 3 cut(s) 429, 441, 1164
BsiHKCI CYCGRG 1 cut(s) 428
BsiSI CCGG 3 cut(s) 183, 297, 347
BslI CCNNNNNNNGG 3 cut(s) 201, 217, 1084
BsmAI GTCTC 2 cut(s) 269, 576
BsnI GGCC 8 cut(s) 119, 215, 221, 231, 251, 362, 380, 1040
Bso31I GGTCTC 1 cut(s) 269
BsoBI CYCGRG 1 cut(s) 428
Bsp1286I GDGCHC 3 cut(s) 429, 441, 1164
Bsp143I GATC 3 cut(s) 11, 1024, 1100
Bsp19I CCATGG 2 cut(s) 114, 858
BspANI GGCC 8 cut(s) 119, 215, 221, 231, 251, 362, 380, 1040
BspCNI CTCAG 4 cut(s) 265, 349, 575, 1138
BspFNI CGCG 2 cut(s) 171, 722
BspLI GGNNCC 1 cut(s) 1041
BspMAI CTGCAG 1 cut(s) 847
BspPI GGATC 2 cut(s) 6, 1032
BspTI CTTAAG 1 cut(s) 461
BspTNI GGTCTC 1 cut(s) 269
BsrDI GCAATG 1 cut(s) 630
BsrFI RCCGGY 1 cut(s) 182
BsrI ACTGG 3 cut(s) 206, 954, 974
BssAI RCCGGY 1 cut(s) 182
BssMI GATC 3 cut(s) 11, 1024, 1100
BssNI GRCGYC 5 cut(s) 51, 123, 186, 287, 386
BssT1I CCWWGG 5 cut(s) 114, 279, 381, 858, 1083
Bst2UI CCWGG 4 cut(s) 164, 212, 218, 248
Bst4CI ACNGT 1 cut(s) 1200
Bst6I CTCTTC 1 cut(s) 1148
BstACI GRCGYC 5 cut(s) 51, 123, 186, 287, 386
BstAFI CTTAAG 1 cut(s) 461
BstC8I GCNNGC 5 cut(s) 169, 207, 335, 622, 1009
BstDEI CTNAG 5 cut(s) 273, 357, 562, 641, 1125
BstDSI CCRYGG 4 cut(s) 114, 363, 720, 858
BstF5I GGATG 3 cut(s) 506, 868, 1074
BstFNI CGCG 2 cut(s) 171, 722
BstHHI GCGC 1 cut(s) 173
BstKTI GATC 3 cut(s) 14, 1027, 1103
BstMAI GTCTC 2 cut(s) 269, 576
BstMBI GATC 3 cut(s) 11, 1024, 1100
BstMCI CGRYCG 1 cut(s) 232
BstNI CCWGG 4 cut(s) 164, 212, 218, 248
BstSCI CCNGG 5 cut(s) 162, 210, 216, 246, 296
BstSFI CTRYAG 1 cut(s) 843
BstUI CGCG 2 cut(s) 171, 722
BstV1I GCAGC 2 cut(s) 355, 973
BstV2I GAAGAC 2 cut(s) 722, 772
BstX2I RGATCY 2 cut(s) 11, 1100
BstXI CCANNNNNNTGG 1 cut(s) 115
BstYI RGATCY 2 cut(s) 11, 1100
BstZI CGGCCG 1 cut(s) 229
BsuI GTATCC 1 cut(s) 466
BsuRI GGCC 8 cut(s) 119, 215, 221, 231, 251, 362, 380, 1040
BtgI CCRYGG 4 cut(s) 114, 363, 720, 858
BtsCI GGATG 3 cut(s) 506, 868, 1074
BtsI GCAGTG 1 cut(s) 852
BtsIMutI CAGTG 3 cut(s) 852, 947, 1186
Cac8I GCNNGC 5 cut(s) 169, 207, 335, 622, 1009
CaiI CAGNNNCTG 1 cut(s) 201
CfoI GCGC 1 cut(s) 173
Cfr10I RCCGGY 1 cut(s) 182
Cfr13I GGNCC 3 cut(s) 214, 378, 1039
Cfr42I CCGCGG 1 cut(s) 723
CseI GACGC 5 cut(s) 59, 131, 175, 276, 375
Csp6I GTAC 3 cut(s) 710, 933, 1201
CviAII CATG 7 cut(s) 115, 468, 514, 683, 859, 874, 964
CviQI GTAC 3 cut(s) 710, 933, 1201
DdeI CTNAG 5 cut(s) 273, 357, 562, 641, 1125
DpnI GATC 3 cut(s) 13, 1026, 1102
DpnII GATC 3 cut(s) 11, 1024, 1100
EaeI YGGCCR 2 cut(s) 117, 229
EagI CGGCCG 1 cut(s) 229
Eam1104I CTCTTC 1 cut(s) 1148
EarI CTCTTC 1 cut(s) 1148
EciI GGCGGA 3 cut(s) 4, 142, 352
Ecl136II GAGCTC 2 cut(s) 427, 439
EclXI CGGCCG 1 cut(s) 229
Eco130I CCWWGG 5 cut(s) 114, 279, 381, 858, 1083
Eco147I AGGCCT 1 cut(s) 251
Eco24I GRGCYC 2 cut(s) 429, 441
Eco31I GGTCTC 1 cut(s) 269
Eco52I CGGCCG 1 cut(s) 229
Eco53kI GAGCTC 2 cut(s) 427, 439
Eco57I CTGAAG 2 cut(s) 519, 734
Eco88I CYCGRG 1 cut(s) 428
EcoICRI GAGCTC 2 cut(s) 427, 439
EcoRII CCWGG 4 cut(s) 162, 210, 216, 246
EcoT14I CCWWGG 5 cut(s) 114, 279, 381, 858, 1083
EcoT38I GRGCYC 2 cut(s) 429, 441
ErhI CCWWGG 5 cut(s) 114, 279, 381, 858, 1083
FaeI CATG 7 cut(s) 118, 471, 517, 686, 862, 877, 967
FatI CATG 7 cut(s) 114, 467, 513, 682, 858, 873, 963
FokI GGATG 3 cut(s) 513, 875, 1061
FriOI GRGCYC 2 cut(s) 429, 441
FspBI CTAG 3 cut(s) 402, 693, 704
GlaI GCGC 1 cut(s) 172
GsuI CTGGAG 1 cut(s) 563
HaeIII GGCC 8 cut(s) 119, 215, 221, 231, 251, 362, 380, 1040
HapII CCGG 3 cut(s) 183, 297, 347
HgaI GACGC 5 cut(s) 59, 131, 175, 276, 375
HhaI GCGC 1 cut(s) 173
Hin1I GRCGYC 5 cut(s) 51, 123, 186, 287, 386
Hin1II CATG 7 cut(s) 118, 471, 517, 686, 862, 877, 967
Hin6I GCGC 1 cut(s) 171
HinP1I GCGC 1 cut(s) 171
HincII GTYRAC 1 cut(s) 871
HindII GTYRAC 1 cut(s) 871
HindIII AAGCTT 1 cut(s) 809
HinfI GANTC 8 cut(s) 74, 293, 373, 455, 627, 696, 839, 1192
HpaII CCGG 3 cut(s) 183, 297, 347
HphI GGTGA 2 cut(s) 184, 1156
Hpy166II GTNNAC 1 cut(s) 871
Hpy188I TCNGA 5 cut(s) 268, 292, 454, 602, 1029
Hpy188III TCNNGA 7 cut(s) 29, 430, 442, 542, 647, 693, 704
Hpy8I GTNNAC 1 cut(s) 871
Hpy99I CGWCG 8 cut(s) 53, 125, 191, 230, 245, 260, 263, 292
HpyAV CCTTC 2 cut(s) 31, 439
HpyCH4III ACNGT 1 cut(s) 1200
HpyCH4IV ACGT 2 cut(s) 586, 787
HpyCH4V TGCA 5 cut(s) 590, 669, 756, 845, 1046
HpyF3I CTNAG 5 cut(s) 273, 357, 562, 641, 1125
HpySE526I ACGT 2 cut(s) 586, 787
Hsp92I GRCGYC 5 cut(s) 51, 123, 186, 287, 386
Hsp92II CATG 7 cut(s) 118, 471, 517, 686, 862, 877, 967
HspAI GCGC 1 cut(s) 171
KspI CCGCGG 1 cut(s) 723
Kzo9I GATC 3 cut(s) 11, 1024, 1100
LmnI GCTCC 2 cut(s) 424, 834
Lsp1109I GCAGC 2 cut(s) 355, 973
LweI GCATC 1 cut(s) 491
MaeI CTAG 3 cut(s) 402, 693, 704
MaeII ACGT 2 cut(s) 586, 787
MaeIII GTNAC 2 cut(s) 190, 523
MalI GATC 3 cut(s) 13, 1026, 1102
MboI GATC 3 cut(s) 11, 1024, 1100
MboII GAAGA 3 cut(s) 727, 777, 1135
MflI RGATCY 2 cut(s) 11, 1100
MhlI GDGCHC 3 cut(s) 429, 441, 1164
MlyI GAGTC 4 cut(s) 68, 302, 705, 833
MmeI TCCRAC 1 cut(s) 270
MroXI GAANNNNTTC 1 cut(s) 305
MseI TTAA 2 cut(s) 327, 462
MslI CAYNNNNRTG 1 cut(s) 113
MspA1I CMGCKG 1 cut(s) 722
MspCI CTTAAG 1 cut(s) 461
MspI CCGG 3 cut(s) 183, 297, 347
MspR9I CCNGG 5 cut(s) 164, 212, 218, 248, 298
MvaI CCWGG 4 cut(s) 164, 212, 218, 248
MvnI CGCG 2 cut(s) 171, 722
NciI CCSGG 1 cut(s) 298
NcoI CCATGG 2 cut(s) 114, 858
NdeII GATC 3 cut(s) 11, 1024, 1100
NlaIII CATG 7 cut(s) 118, 471, 517, 686, 862, 877, 967
NlaIV GGNNCC 1 cut(s) 1041
NmuCI GTSAC 1 cut(s) 190
PaeR7I CTCGAG 1 cut(s) 428
PceI AGGCCT 1 cut(s) 251
PcsI WCGNNNNNNNCGW 2 cut(s) 195, 264
PdmI GAANNNNTTC 1 cut(s) 305
PfeI GAWTC 4 cut(s) 373, 455, 627, 1192
PflMI CCANNNNNTGG 1 cut(s) 201
PfoI TCCNGGA 1 cut(s) 296
PleI GAGTC 4 cut(s) 68, 301, 704, 833
PpsI GAGTC 4 cut(s) 68, 301, 704, 833
PsiI TTATAA 1 cut(s) 920
Psp124BI GAGCTC 2 cut(s) 429, 441
Psp6I CCWGG 4 cut(s) 162, 210, 216, 246
PspGI CCWGG 4 cut(s) 162, 210, 216, 246
PspN4I GGNNCC 1 cut(s) 1041
PspPI GGNCC 3 cut(s) 214, 378, 1039
PstI CTGCAG 1 cut(s) 847
PstNI CAGNNNCTG 1 cut(s) 201
PsuI RGATCY 2 cut(s) 11, 1100
RsaI GTAC 3 cut(s) 711, 934, 1202
RsaNI GTAC 3 cut(s) 710, 933, 1201
RseI CAYNNNNRTG 1 cut(s) 113
SacI GAGCTC 2 cut(s) 429, 441
SacII CCGCGG 1 cut(s) 723
SaqAI TTAA 2 cut(s) 327, 462
Sau3AI GATC 3 cut(s) 11, 1024, 1100
Sau96I GGNCC 3 cut(s) 214, 378, 1039
ScaI AGTACT 1 cut(s) 711
SchI GAGTC 4 cut(s) 68, 302, 705, 833
ScrFI CCNGG 5 cut(s) 164, 212, 218, 248, 298
SduI GDGCHC 3 cut(s) 429, 441, 1164
SfaNI GCATC 1 cut(s) 491
SfcI CTRYAG 1 cut(s) 843
Sfr274I CTCGAG 1 cut(s) 428
Sfr303I CCGCGG 1 cut(s) 723
SgrAI CRCCGGYG 1 cut(s) 182
SgrBI CCGCGG 1 cut(s) 723
SlaI CTCGAG 1 cut(s) 428
SmiMI CAYNNNNRTG 1 cut(s) 113
SmlI CTYRAG 3 cut(s) 428, 440, 461
SmoI CTYRAG 3 cut(s) 428, 440, 461
SseBI AGGCCT 1 cut(s) 251
SspMI CTAG 3 cut(s) 402, 693, 704
SstI GAGCTC 2 cut(s) 429, 441
StuI AGGCCT 1 cut(s) 251
StyD4I CCNGG 5 cut(s) 162, 210, 216, 246, 296
StyI CCWWGG 5 cut(s) 114, 279, 381, 858, 1083
TaaI ACNGT 1 cut(s) 1200
TaiI ACGT 2 cut(s) 589, 790
TaqI TCGA 6 cut(s) 30, 37, 318, 429, 529, 745
TatI WGTACW 2 cut(s) 709, 1200
TauI GCSGC 8 cut(s) 56, 128, 176, 224, 234, 287, 371, 725
TfiI GAWTC 4 cut(s) 373, 455, 627, 1192
Tru1I TTAA 2 cut(s) 327, 462
Tru9I TTAA 2 cut(s) 327, 462
TscAI CASTG 3 cut(s) 852, 954, 1186
TseFI GTSAC 1 cut(s) 190
TseI GCWGC 2 cut(s) 343, 986
Tsp45I GTSAC 1 cut(s) 190
TspDTI ATGAA 4 cut(s) 13, 502, 671, 980
TspGWI ACGGA 1 cut(s) 244
TspRI CASTG 3 cut(s) 852, 954, 1186
Van91I CCANNNNNTGG 1 cut(s) 201
Vha464I CTTAAG 1 cut(s) 461
XbaI TCTAGA 2 cut(s) 692, 703
XhoI CTCGAG 1 cut(s) 428
XmnI GAANNNNTTC 1 cut(s) 305
XspI CTAG 3 cut(s) 402, 693, 704
ZrmI AGTACT 1 cut(s) 711
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.