MD17G1153700.v1.1

Belongs to the eIF-2B alpha beta delta subunits family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0006573
Physical Location & Seq
Reverse (-)
14498749 .. 14501955
3207 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1153700.v1.1.491

Sequence Viewer

Length: 1209 bp
ATGTGGTGGAGATCAGCCTCCTTCATTCTCGACAAGCAGGGCCAGGAGCGAAACGACGCCGTATTGGCCTCGAAACCCGATTCCCTCACTCTCTCCCCGCCACCACCGCCTTCGTCCATGGCTGACGCTCTGCAGAGCCCTAACCCTAAGATATCAGCCTACTACCAGACCCGGGCGGCCCACCACGGCGTCGTCACCAGCGACTGGCTGGCCCAGGCCCAAGCTGCCGTGGGCAGAAACCCCGACGACCAAGGGCAAATCGGCGCCGAGGTTGAAGCTAAGTCTGGGTCTAATTCCGGCAAGCCCTTCAGCGTCATCGAGGAGTTCAATAGCTGGCGAAAGCAGCCCGATTTGGCGGAGGCCGTGGCGGCGATTCGGGCCTTAGCGTCTGTGATTCGGTCCAGTGAGGCCACCACTATGATGGAGCTCGAAATTGAGCTAAAGAAGGCCTCCGAGTCTCTTAAATCGTGGGACACGACCTCTATTTCTTTGACTGCTGGATGCGACCTGTTCATCCGGTATGTTACTCGAACTTCGGCTTTAGAGTATGAGGACTTCAACTCAGCCAAGTCTCGTTTGATTGAACGTGCAGAGAAGTTTGGGGAGATATCTATGAAGGCCCGCAGAATCATTGCTGTGCTTAGTCAAGACTTTATATTTGATGGTTGCACCATATTGGTTCACGGTTTCTCTAGAGTTGTTCTAGAAGTATTAAAGACTGCGGCACAGAGCAACAAACTCTTTCGAGTTTTCTGTACAGAGGGAAGACCAGACAGAACAGGATTACGATTGTCAAATGAGCTGGCCAAGCTTGATGTTCCTGTGAAGCTTCTAATAGACTCTGCAGCAGCATATACCATGGATGAGGTCGACATGGTATTTGTTGGGGCAGATGGAGTGGTTGAAAGTGGAGGTATCATCAATATGATGGGAACATTTCAAATTGCCTTAGTGGCACACAGCATGAACAAACCGGTTTATGTGGCTGCTGAAAGCTACAAGTTTGCTCGCCTTTATCCATTGGACCAGAAAGACATGGCCCCTGCATTACATCCCATTGATTTTGGGGTACCCATCCCATCCAAGGTTGAAGTTGAAAGGTCTGCCCGTGATTATACTCCTCCGCAGTATCTTACTCTGCTCTTCACGGATTTGGGCGTACTAACTCCGTCGGTGGTCAGCGATGAGCTTATTCAGCTATACTTGTGA

Protein Analysis

403

Amino Acids

44.16

Weight (kDa)

5.26

Isoelectric Point (pI)

40.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
IF-2B PF01008 115 - 390 6.9e-71 Initiation factor 2 subunit family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1069
AccB1I GGYRCC 2 cut(s) 263, 1069
AccB7I CCANNNNNTGG 1 cut(s) 204
AccI GTMKAC 1 cut(s) 870
AciI CCGC 8 cut(s) 98, 107, 176, 356, 368, 622, 722, 1124
AcoI YGGCCR 1 cut(s) 804
AcuI CTGAAG 1 cut(s) 292
AcyI GRCGYC 3 cut(s) 57, 189, 264
AfaI GTAC 3 cut(s) 757, 1071, 1161
AfiI CCNNNNNNNGG 3 cut(s) 172, 204, 1084
AgeI ACCGGT 1 cut(s) 973
AgsI TTSAA 8 cut(s) 275, 328, 559, 584, 905, 941, 1091, 1097
AjnI CCWGG 2 cut(s) 42, 213
Alw21I GWGCWC 1 cut(s) 429
Alw26I GTCTC 2 cut(s) 462, 576
AlwNI CAGNNNCTG 1 cut(s) 204
Ama87I CYCGRG 1 cut(s) 171
ApeKI GCWGC 5 cut(s) 224, 343, 845, 848, 986
ArsI GACNNNNNNTTYG 4 cut(s) 641, 673, 863, 895
AsiGI ACCGGT 1 cut(s) 973
Asp718I GGTACC 1 cut(s) 1069
AspLEI GCGC 1 cut(s) 266
AspS9I GGNCC 9 cut(s) 40, 178, 211, 217, 378, 399, 619, 1024, 1039
AsuC2I CCSGG 2 cut(s) 172, 173
AsuHPI GGTGA 1 cut(s) 187
AvaI CYCGRG 1 cut(s) 171
AvaII GGWCC 2 cut(s) 399, 1024
BalI TGGCCA 1 cut(s) 806
BanI GGYRCC 2 cut(s) 263, 1069
BanII GRGCYC 2 cut(s) 140, 429
BarI GAAGNNNNNNTAC 2 cut(s) 539, 571
BbsI GAAGAC 1 cut(s) 772
Bbv12I GWGCWC 1 cut(s) 429
BbvI GCAGC 5 cut(s) 211, 355, 857, 860, 973
BccI CCATC 6 cut(s) 415, 656, 887, 922, 1082, 1087
BceAI ACGGC 4 cut(s) 44, 202, 212, 347
BciT130I CCWGG 2 cut(s) 44, 215
BcnI CCSGG 2 cut(s) 172, 173
BcoDI GTCTC 2 cut(s) 462, 576
BfaI CTAG 2 cut(s) 693, 704
BfmI CTRYAG 2 cut(s) 131, 843
BfoI RGCGCY 1 cut(s) 267
BglI GCCNNNNNGGC 3 cut(s) 65, 368, 953
BisI GCNGC 8 cut(s) 177, 225, 344, 369, 723, 846, 849, 987
BlsI GCNGC 8 cut(s) 178, 226, 345, 370, 724, 847, 850, 988
Bme1390I CCNGG 4 cut(s) 44, 172, 173, 215
Bme18I GGWCC 2 cut(s) 399, 1024
BmeT110I CYCGRG 1 cut(s) 171
BmgT120I GGNCC 9 cut(s) 40, 178, 211, 217, 378, 399, 619, 1024, 1039
BmiI GGNNCC 3 cut(s) 265, 1041, 1071
BmrFI CCNGG 4 cut(s) 44, 172, 173, 215
BmsI GCATC 1 cut(s) 491
BpiI GAAGAC 1 cut(s) 772
Bpu10I CCTNAGC 1 cut(s) 382
BpuMI CCSGG 2 cut(s) 172, 173
BsaHI GRCGYC 3 cut(s) 57, 189, 264
BsaWI WCCGGW 2 cut(s) 516, 973
Bsc4I CCNNNNNNNGG 3 cut(s) 172, 204, 1084
Bse118I RCCGGY 1 cut(s) 973
Bse1I ACTGG 2 cut(s) 209, 402
Bse3DI GCAATG 1 cut(s) 630
BseBI CCWGG 2 cut(s) 44, 215
BseGI GGATG 6 cut(s) 506, 513, 868, 1051, 1074, 1079
BseLI CCNNNNNNNGG 3 cut(s) 172, 204, 1084
BseMI GCAATG 1 cut(s) 630
BseMII CTCAG 1 cut(s) 576
BseNI ACTGG 2 cut(s) 209, 402
BseRI GAGGAG 2 cut(s) 335, 1110
BseXI GCAGC 5 cut(s) 211, 355, 857, 860, 973
BsgI GTGCAG 1 cut(s) 609
BshNI GGYRCC 2 cut(s) 263, 1069
BshTI ACCGGT 1 cut(s) 973
BsiHKAI GWGCWC 1 cut(s) 429
BsiHKCI CYCGRG 1 cut(s) 171
BsiSI CCGG 4 cut(s) 172, 297, 517, 974
BslFI GGGAC 1 cut(s) 485
BslI CCNNNNNNNGG 3 cut(s) 172, 204, 1084
BsmAI GTCTC 2 cut(s) 462, 576
BsmFI GGGAC 1 cut(s) 485
BsoBI CYCGRG 1 cut(s) 171
Bsp1286I GDGCHC 2 cut(s) 140, 429
Bsp1407I TGTACA 1 cut(s) 755
Bsp143I GATC 1 cut(s) 11
Bsp19I CCATGG 2 cut(s) 117, 858
BspACI CCGC 8 cut(s) 98, 107, 176, 356, 368, 622, 722, 1124
BspCNI CTCAG 1 cut(s) 575
BspLI GGNNCC 3 cut(s) 265, 1041, 1071
BspMAI CTGCAG 2 cut(s) 135, 847
BspQI GCTCTTC 1 cut(s) 1148
BspT107I GGYRCC 2 cut(s) 263, 1069
BsrDI GCAATG 1 cut(s) 630
BsrFI RCCGGY 1 cut(s) 973
BsrGI TGTACA 1 cut(s) 755
BsrI ACTGG 2 cut(s) 209, 402
BssAI RCCGGY 1 cut(s) 973
BssMI GATC 1 cut(s) 11
BssNI GRCGYC 3 cut(s) 57, 189, 264
BssT1I CCWWGG 4 cut(s) 117, 250, 858, 1083
Bst2UI CCWGG 2 cut(s) 44, 215
Bst4CI ACNGT 1 cut(s) 686
Bst6I CTCTTC 1 cut(s) 1148
BstACI GRCGYC 3 cut(s) 57, 189, 264
BstAUI TGTACA 1 cut(s) 755
BstC8I GCNNGC 6 cut(s) 210, 302, 335, 622, 804, 1009
BstDEI CTNAG 6 cut(s) 147, 279, 382, 562, 641, 949
BstDSI CCRYGG 5 cut(s) 117, 184, 228, 363, 858
BstF5I GGATG 6 cut(s) 506, 513, 868, 1051, 1074, 1079
BstH2I RGCGCY 1 cut(s) 267
BstHHI GCGC 1 cut(s) 266
BstKTI GATC 1 cut(s) 14
BstMAI GTCTC 2 cut(s) 462, 576
BstMBI GATC 1 cut(s) 11
BstMWI GCNNNNNNNGC 9 cut(s) 65, 106, 224, 343, 368, 377, 808, 953, 1195
BstNI CCWGG 2 cut(s) 44, 215
BstSCI CCNGG 4 cut(s) 42, 170, 171, 213
BstSFI CTRYAG 2 cut(s) 131, 843
BstV1I GCAGC 5 cut(s) 211, 355, 857, 860, 973
BstV2I GAAGAC 1 cut(s) 772
BstXI CCANNNNNNTGG 1 cut(s) 421
BtgI CCRYGG 5 cut(s) 117, 184, 228, 363, 858
BtgZI GCGATG 1 cut(s) 1197
BtsCI GGATG 6 cut(s) 506, 513, 868, 1051, 1074, 1079
BtsIMutI CAGTG 1 cut(s) 409
Cac8I GCNNGC 6 cut(s) 210, 302, 335, 622, 804, 1009
CaiI CAGNNNCTG 1 cut(s) 204
CfoI GCGC 1 cut(s) 266
Cfr10I RCCGGY 1 cut(s) 973
Cfr13I GGNCC 9 cut(s) 40, 178, 211, 217, 378, 399, 619, 1024, 1039
Cfr9I CCCGGG 1 cut(s) 171
CseI GACGC 5 cut(s) 65, 134, 178, 301, 375
Csp6I GTAC 3 cut(s) 756, 1070, 1160
CspAI ACCGGT 1 cut(s) 973
CviAII CATG 5 cut(s) 118, 859, 874, 964, 1036
CviQI GTAC 3 cut(s) 756, 1070, 1160
DdeI CTNAG 6 cut(s) 147, 279, 382, 562, 641, 949
DinI GGCGCC 1 cut(s) 265
DpnI GATC 1 cut(s) 13
DpnII GATC 1 cut(s) 11
EaeI YGGCCR 1 cut(s) 804
Eam1104I CTCTTC 1 cut(s) 1148
EarI CTCTTC 1 cut(s) 1148
EciI GGCGGA 1 cut(s) 371
Ecl136II GAGCTC 1 cut(s) 427
Eco130I CCWWGG 4 cut(s) 117, 250, 858, 1083
Eco147I AGGCCT 1 cut(s) 449
Eco24I GRGCYC 2 cut(s) 140, 429
Eco32I GATATC 2 cut(s) 153, 609
Eco47I GGWCC 2 cut(s) 399, 1024
Eco53kI GAGCTC 1 cut(s) 427
Eco57I CTGAAG 1 cut(s) 292
Eco88I CYCGRG 1 cut(s) 171
EcoICRI GAGCTC 1 cut(s) 427
EcoRII CCWGG 2 cut(s) 42, 213
EcoRV GATATC 2 cut(s) 153, 609
EcoT14I CCWWGG 4 cut(s) 117, 250, 858, 1083
EcoT38I GRGCYC 2 cut(s) 140, 429
EgeI GGCGCC 1 cut(s) 265
EheI GGCGCC 1 cut(s) 265
ErhI CCWWGG 4 cut(s) 117, 250, 858, 1083
FaeI CATG 5 cut(s) 121, 862, 877, 967, 1039
FaqI GGGAC 1 cut(s) 485
FatI CATG 5 cut(s) 117, 858, 873, 963, 1035
FauI CCCGC 2 cut(s) 105, 629
FblI GTMKAC 1 cut(s) 870
Fnu4HI GCNGC 8 cut(s) 177, 225, 344, 369, 723, 846, 849, 987
FokI GGATG 6 cut(s) 500, 513, 875, 1038, 1061, 1066
FriOI GRGCYC 2 cut(s) 140, 429
Fsp4HI GCNGC 8 cut(s) 177, 225, 344, 369, 723, 846, 849, 987
FspBI CTAG 2 cut(s) 693, 704
GlaI GCGC 1 cut(s) 265
GluI GCNGC 8 cut(s) 177, 225, 344, 369, 723, 846, 849, 987
HaeII RGCGCY 1 cut(s) 267
HapII CCGG 4 cut(s) 172, 297, 517, 974
HgaI GACGC 5 cut(s) 65, 134, 178, 301, 375
HhaI GCGC 1 cut(s) 266
Hin1I GRCGYC 3 cut(s) 57, 189, 264
Hin1II CATG 5 cut(s) 121, 862, 877, 967, 1039
Hin6I GCGC 1 cut(s) 264
HinP1I GCGC 1 cut(s) 264
HincII GTYRAC 1 cut(s) 871
HindII GTYRAC 1 cut(s) 871
HindIII AAGCTT 2 cut(s) 809, 827
HinfI GANTC 6 cut(s) 80, 373, 394, 455, 627, 839
HpaII CCGG 4 cut(s) 172, 297, 517, 974
HphI GGTGA 1 cut(s) 187
Hpy166II GTNNAC 2 cut(s) 682, 871
Hpy188I TCNGA 1 cut(s) 454
Hpy188III TCNNGA 4 cut(s) 29, 647, 693, 704
Hpy8I GTNNAC 2 cut(s) 682, 871
Hpy99I CGWCG 4 cut(s) 59, 194, 248, 1174
HpyAV CCTTC 5 cut(s) 31, 120, 316, 439, 610
HpyCH4III ACNGT 1 cut(s) 686
HpyCH4IV ACGT 1 cut(s) 586
HpyCH4V TGCA 5 cut(s) 133, 590, 669, 845, 1046
HpyF10VI GCNNNNNNNGC 9 cut(s) 65, 106, 224, 343, 368, 377, 808, 953, 1195
HpyF3I CTNAG 6 cut(s) 147, 279, 382, 562, 641, 949
HpySE526I ACGT 1 cut(s) 586
Hsp92I GRCGYC 3 cut(s) 57, 189, 264
Hsp92II CATG 5 cut(s) 121, 862, 877, 967, 1039
HspAI GCGC 1 cut(s) 264
KasI GGCGCC 1 cut(s) 263
KpnI GGTACC 1 cut(s) 1073
Kzo9I GATC 1 cut(s) 11
LguI GCTCTTC 1 cut(s) 1148
LmnI GCTCC 2 cut(s) 46, 424
Lsp1109I GCAGC 5 cut(s) 211, 355, 857, 860, 973
LweI GCATC 1 cut(s) 491
MaeI CTAG 2 cut(s) 693, 704
MaeII ACGT 1 cut(s) 586
MaeIII GTNAC 2 cut(s) 193, 523
MalI GATC 1 cut(s) 13
MboI GATC 1 cut(s) 11
MboII GAAGA 2 cut(s) 777, 1135
MhlI GDGCHC 2 cut(s) 140, 429
MlsI TGGCCA 1 cut(s) 806
MluCI AATT 3 cut(s) 292, 432, 942
MluNI TGGCCA 1 cut(s) 806
Mly113I GGCGCC 1 cut(s) 264
MlyI GAGTC 2 cut(s) 464, 833
Mox20I TGGCCA 1 cut(s) 806
MscI TGGCCA 1 cut(s) 806
MseI TTAA 2 cut(s) 462, 713
MslI CAYNNNNRTG 4 cut(s) 416, 419, 635, 923
Msp20I TGGCCA 1 cut(s) 806
MspI CCGG 4 cut(s) 172, 297, 517, 974
MspR9I CCNGG 4 cut(s) 44, 172, 173, 215
MvaI CCWGG 2 cut(s) 44, 215
MwoI GCNNNNNNNGC 9 cut(s) 65, 106, 224, 343, 368, 377, 808, 953, 1195
NarI GGCGCC 1 cut(s) 264
NciI CCSGG 2 cut(s) 172, 173
NcoI CCATGG 2 cut(s) 117, 858
NdeII GATC 1 cut(s) 11
NlaIII CATG 5 cut(s) 121, 862, 877, 967, 1039
NlaIV GGNNCC 3 cut(s) 265, 1041, 1071
NmeAIII GCCGAG 1 cut(s) 292
NmuCI GTSAC 1 cut(s) 193
PceI AGGCCT 1 cut(s) 449
PciSI GCTCTTC 1 cut(s) 1148
PcsI WCGNNNNNNNCGW 2 cut(s) 198, 473
PfeI GAWTC 4 cut(s) 80, 373, 394, 627
PflMI CCANNNNNTGG 1 cut(s) 204
PinAI ACCGGT 1 cut(s) 973
PkrI GCNGC 8 cut(s) 178, 226, 345, 370, 724, 847, 850, 988
PleI GAGTC 2 cut(s) 463, 833
PluTI GGCGCC 1 cut(s) 267
PpsI GAGTC 2 cut(s) 463, 833
Psp124BI GAGCTC 1 cut(s) 429
Psp6I CCWGG 2 cut(s) 42, 213
PspGI CCWGG 2 cut(s) 42, 213
PspN4I GGNNCC 3 cut(s) 265, 1041, 1071
PspPI GGNCC 9 cut(s) 40, 178, 211, 217, 378, 399, 619, 1024, 1039
PstI CTGCAG 2 cut(s) 135, 847
PstNI CAGNNNCTG 1 cut(s) 204
RsaI GTAC 3 cut(s) 757, 1071, 1161
RsaNI GTAC 3 cut(s) 756, 1070, 1160
RseI CAYNNNNRTG 4 cut(s) 416, 419, 635, 923
SacI GAGCTC 1 cut(s) 429
SalI GTCGAC 1 cut(s) 869
SapI GCTCTTC 1 cut(s) 1148
SaqAI TTAA 2 cut(s) 462, 713
SatI GCNGC 8 cut(s) 177, 225, 344, 369, 723, 846, 849, 987
Sau3AI GATC 1 cut(s) 11
Sau96I GGNCC 9 cut(s) 40, 178, 211, 217, 378, 399, 619, 1024, 1039
SchI GAGTC 2 cut(s) 464, 833
ScrFI CCNGG 4 cut(s) 44, 172, 173, 215
SduI GDGCHC 2 cut(s) 140, 429
SfaNI GCATC 1 cut(s) 491
SfcI CTRYAG 2 cut(s) 131, 843
SfoI GGCGCC 1 cut(s) 265
SinI GGWCC 2 cut(s) 399, 1024
SmaI CCCGGG 1 cut(s) 173
SmiMI CAYNNNNRTG 4 cut(s) 416, 419, 635, 923
Sse9I AATT 3 cut(s) 292, 432, 942
SseBI AGGCCT 1 cut(s) 449
SsiI CCGC 8 cut(s) 98, 107, 176, 356, 368, 622, 722, 1124
SspDI GGCGCC 1 cut(s) 263
SspMI CTAG 2 cut(s) 693, 704
SstI GAGCTC 1 cut(s) 429
StuI AGGCCT 1 cut(s) 449
StyD4I CCNGG 4 cut(s) 42, 170, 171, 213
StyI CCWWGG 4 cut(s) 117, 250, 858, 1083
TaaI ACNGT 1 cut(s) 686
TaiI ACGT 1 cut(s) 589
TaqI TCGA 7 cut(s) 30, 71, 318, 429, 529, 745, 870
TaqII GACCGA 1 cut(s) 387
TasI AATT 3 cut(s) 292, 432, 942
TatI WGTACW 1 cut(s) 755
TauI GCSGC 3 cut(s) 179, 371, 725
TfiI GAWTC 4 cut(s) 80, 373, 394, 627
Tru1I TTAA 2 cut(s) 462, 713
Tru9I TTAA 2 cut(s) 462, 713
TscAI CASTG 1 cut(s) 409
TseFI GTSAC 1 cut(s) 193
TseI GCWGC 5 cut(s) 224, 343, 845, 848, 986
Tsp45I GTSAC 1 cut(s) 193
TspDTI ATGAA 4 cut(s) 13, 502, 629, 980
TspGWI ACGGA 2 cut(s) 1158, 1163
TspMI CCCGGG 1 cut(s) 171
TspRI CASTG 1 cut(s) 409
Van91I CCANNNNNTGG 1 cut(s) 204
VpaK11BI GGWCC 2 cut(s) 399, 1024
XbaI TCTAGA 2 cut(s) 692, 703
XcmI CCANNNNNNNNNTGG 2 cut(s) 205, 418
XmaI CCCGGG 1 cut(s) 171
XmiI GTMKAC 1 cut(s) 870
XspI CTAG 2 cut(s) 693, 704
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.