RLG00000020276

Belongs to the eIF-2B alpha beta delta subunits family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0006573
Physical Location & Seq
Reverse (-)
63142450 .. 63144909
2460 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000020276

Sequence Viewer

Length: 1197 bp
ATGTGGTGGAGATCCGCCTCCTTCATTCTCGACAACCGTCCCCAAAACGACGTCGCACCGCCCTCCAGACCCGACTCCCTCACTCTCTCCCCACCCACCATGGCCGACGCCGCCCTCCAAAGCCCTAACGCCAACTCCGCCTACTACCAGACCCGGGCCGCCCACCACGGCGTCGTCACCAGCGACTGGCTCGCCCAGGCCCAGGCCGCCGTGGGCCGAAACCCCGACGAGCAGGGCTCCGACGTCGCCGCCGAGGCTGAGCCCAGGCCGGCGGCTTCTGAGTCCGGGAAGCCGTTCAGCGTCATCGTGGAGTTTAATAACTGGCGGAAGCAGCCTGATTTGGCTGAGGCCGTGGCGGCGATTCGGGCCTTGGCGTCTGTTATAAGGTCCAGCGAGGCCACCACTATGATGGAGCTCGAAATTGAGCTCAAGAAGGCTTCCGATTCTCTTAAGTCATGGGATACGACGTCTATATCTTTGACTGCTGGGTGCGACCTGTTTATGCGGTATGTTACTCGAACTTCAGCTCTAGAGTATGAGGATTTCCACTCAGCCAAGTCTCGTTTGATTGAACGGGCAGAGAAGTTTGGGGAGATATCTACAAAGGCTCGTAGAATCATTGCTGTCCTTAGTCAAGACTTTATATTTGATGGTTGTACCATCTTGGTTCATGGTTTCTCTAGAGTCGTTCTAGAAGTACTGAAGACTGCGGCACAGAGCAAGAAACTCTTTCGAGTTTTCTGCACAGAGGGAAGACCAGACAGAACAGGATTACGGTTATCAAATGAGCTGGCCAAGCTTGATGTTCCTGTGAAGCTCCTAATAGATTCTGCAGTGGCATATACCATGGATGAGGTTGACATGGTGTTTGTTGGGGCAGATGGAGTGGTTGAAAGTGGAGGTATTATAAATATGATGGGAACATATCAAATTGCATTGGTGGCACATAGCATGAACAAACCAGTTTATGTGGCTGCTGAAAGCTACAAGTTCGCTCGCCTTTATCCGTTGGACCAGAAAGACATGGCCCCTGCATTACGCCCCATTGATTTTGGGGTTCCCATCCCGTCCAAGGTTGAGGTTGAAAGATCTGCCCGTGATTATACGCCTCCTCAATATCTCACTTTACTCTTCACAGACTTGGGCGTGCTCACTCCATCGGTTGTCAGTGATGAGCTGATTCAGCTGTACTTGTAA

Protein Analysis

399

Amino Acids

43.82

Weight (kDa)

5.25

Isoelectric Point (pI)

40.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
IF-2B PF01008 111 - 386 2.5e-72 Initiation factor 2 subunit family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 383, 908
AatII GACGTC 3 cut(s) 54, 246, 470
AccB7I CCANNNNNTGG 1 cut(s) 186
AclWI GGATC 1 cut(s) 6
AcoI YGGCCR 2 cut(s) 102, 792
AcuI CTGAAG 2 cut(s) 507, 722
AcyI GRCGYC 6 cut(s) 51, 108, 171, 243, 374, 467
AfaI GTAC 3 cut(s) 658, 699, 1190
AfiI CCNNNNNNNGG 4 cut(s) 154, 186, 202, 1072
AflII CTTAAG 1 cut(s) 449
AgsI TTSAA 3 cut(s) 572, 893, 1085
AjnI CCWGG 3 cut(s) 195, 201, 263
AluBI AGCT 9 cut(s) 415, 427, 527, 790, 799, 817, 984, 1177, 1186
AluI AGCT 9 cut(s) 415, 427, 527, 790, 799, 817, 984, 1177, 1186
Alw21I GWGCWC 3 cut(s) 417, 429, 1152
Alw26I GTCTC 1 cut(s) 564
AlwI GGATC 1 cut(s) 6
AlwNI CAGNNNCTG 1 cut(s) 186
Ama87I CYCGRG 1 cut(s) 153
ApeKI GCWGC 2 cut(s) 331, 974
ArsI GACNNNNNNTTYG 4 cut(s) 629, 661, 851, 883
Asp700I GAANNNNTTC 1 cut(s) 293
AspS9I GGNCC 7 cut(s) 156, 199, 214, 366, 387, 1012, 1027
AsuC2I CCSGG 3 cut(s) 154, 155, 286
AsuHPI GGTGA 1 cut(s) 169
AvaI CYCGRG 1 cut(s) 153
AvaII GGWCC 2 cut(s) 387, 1012
BalI TGGCCA 1 cut(s) 794
BanII GRGCYC 4 cut(s) 239, 264, 417, 429
BbsI GAAGAC 2 cut(s) 710, 760
Bbv12I GWGCWC 3 cut(s) 417, 429, 1152
BbvCI CCTCAGC 1 cut(s) 345
BbvI GCAGC 2 cut(s) 343, 961
BccI CCATC 7 cut(s) 403, 644, 668, 875, 910, 1070, 1165
BceAI ACGGC 4 cut(s) 184, 194, 277, 335
BcgI CGANNNNNNTGC 2 cut(s) 723, 757
BciT130I CCWGG 3 cut(s) 197, 203, 265
BciVI GTATCC 1 cut(s) 454
BcnI CCSGG 3 cut(s) 154, 155, 286
BcoDI GTCTC 1 cut(s) 564
BfaI CTAG 3 cut(s) 530, 681, 692
BfmI CTRYAG 1 cut(s) 831
BfrI CTTAAG 1 cut(s) 449
BfuI GTATCC 1 cut(s) 454
BglI GCCNNNNNGGC 2 cut(s) 254, 356
BglII AGATCT 1 cut(s) 1088
BisI GCNGC 9 cut(s) 111, 159, 207, 249, 273, 332, 357, 711, 975
BlpI GCTNAGC 1 cut(s) 258
BlsI GCNGC 9 cut(s) 112, 160, 208, 250, 274, 333, 358, 712, 976
BmcAI AGTACT 1 cut(s) 699
Bme1390I CCNGG 6 cut(s) 154, 155, 197, 203, 265, 286
Bme18I GGWCC 2 cut(s) 387, 1012
BmeT110I CYCGRG 1 cut(s) 153
BmgT120I GGNCC 7 cut(s) 156, 199, 214, 366, 387, 1012, 1027
BmiI GGNNCC 3 cut(s) 238, 1029, 1059
BmrFI CCNGG 6 cut(s) 154, 155, 197, 203, 265, 286
BoxI GACNNNNGTC 1 cut(s) 36
BpiI GAAGAC 2 cut(s) 710, 760
BplI GAGNNNNNCTC 2 cut(s) 221, 253
BpmI CTGGAG 1 cut(s) 49
Bpu10I CCTNAGC 1 cut(s) 345
Bpu1102I GCTNAGC 1 cut(s) 258
BpuEI CTTGAG 1 cut(s) 413
BpuMI CCSGG 3 cut(s) 154, 155, 286
BsaHI GRCGYC 6 cut(s) 51, 108, 171, 243, 374, 467
BsaXI ACNNNNNCTCC 2 cut(s) 119, 149
Bsc4I CCNNNNNNNGG 4 cut(s) 154, 186, 202, 1072
Bse118I RCCGGY 1 cut(s) 268
Bse1I ACTGG 3 cut(s) 191, 326, 962
Bse3DI GCAATG 1 cut(s) 618
BseBI CCWGG 3 cut(s) 197, 203, 265
BseGI GGATG 2 cut(s) 856, 1062
BseLI CCNNNNNNNGG 4 cut(s) 154, 186, 202, 1072
BseMI GCAATG 1 cut(s) 618
BseMII CTCAG 4 cut(s) 249, 270, 336, 564
BseNI ACTGG 3 cut(s) 191, 326, 962
BseRI GAGGAG 1 cut(s) 1101
BseXI GCAGC 2 cut(s) 343, 961
BseYI CCCAGC 1 cut(s) 485
BsgI GTGCAG 1 cut(s) 727
BsiHKAI GWGCWC 3 cut(s) 417, 429, 1152
BsiHKCI CYCGRG 1 cut(s) 153
BsiSI CCGG 3 cut(s) 154, 269, 285
BslFI GGGAC 1 cut(s) 24
BslI CCNNNNNNNGG 4 cut(s) 154, 186, 202, 1072
BsmAI GTCTC 1 cut(s) 564
BsmFI GGGAC 1 cut(s) 24
BsoBI CYCGRG 1 cut(s) 153
Bsp1286I GDGCHC 5 cut(s) 239, 264, 417, 429, 1152
Bsp143I GATC 2 cut(s) 11, 1088
Bsp1720I GCTNAGC 1 cut(s) 258
Bsp19I CCATGG 2 cut(s) 99, 846
BspCNI CTCAG 4 cut(s) 250, 271, 337, 563
BspLI GGNNCC 3 cut(s) 238, 1029, 1059
BspMAI CTGCAG 1 cut(s) 835
BspPI GGATC 1 cut(s) 6
BspTI CTTAAG 1 cut(s) 449
BsrDI GCAATG 1 cut(s) 618
BsrFI RCCGGY 1 cut(s) 268
BsrI ACTGG 3 cut(s) 191, 326, 962
BssAI RCCGGY 1 cut(s) 268
BssMI GATC 2 cut(s) 11, 1088
BssNI GRCGYC 6 cut(s) 51, 108, 171, 243, 374, 467
BssT1I CCWWGG 4 cut(s) 99, 369, 846, 1071
Bst2UI CCWGG 3 cut(s) 197, 203, 265
Bst4CI ACNGT 2 cut(s) 38, 777
Bst6I CTCTTC 1 cut(s) 1136
BstACI GRCGYC 6 cut(s) 51, 108, 171, 243, 374, 467
BstAFI CTTAAG 1 cut(s) 449
BstC8I GCNNGC 5 cut(s) 192, 270, 792, 997, 1148
BstDEI CTNAG 5 cut(s) 258, 279, 345, 550, 629
BstDSI CCRYGG 5 cut(s) 99, 166, 210, 351, 846
BstF5I GGATG 2 cut(s) 856, 1062
BstKTI GATC 2 cut(s) 14, 1091
BstMAI GTCTC 1 cut(s) 564
BstMBI GATC 2 cut(s) 11, 1088
BstNI CCWGG 3 cut(s) 197, 203, 265
BstPAI GACNNNNGTC 1 cut(s) 36
BstSCI CCNGG 6 cut(s) 152, 153, 195, 201, 263, 284
BstSFI CTRYAG 1 cut(s) 831
BstV1I GCAGC 2 cut(s) 343, 961
BstV2I GAAGAC 2 cut(s) 710, 760
BstX2I RGATCY 2 cut(s) 11, 1088
BstXI CCANNNNNNTGG 1 cut(s) 409
BstYI RGATCY 2 cut(s) 11, 1088
BsuI GTATCC 1 cut(s) 454
BtgI CCRYGG 5 cut(s) 99, 166, 210, 351, 846
BtsCI GGATG 2 cut(s) 856, 1062
BtsI GCAGTG 1 cut(s) 840
BtsIMutI CAGTG 2 cut(s) 840, 1174
Cac8I GCNNGC 5 cut(s) 192, 270, 792, 997, 1148
CaiI CAGNNNCTG 1 cut(s) 186
Cfr10I RCCGGY 1 cut(s) 268
Cfr13I GGNCC 7 cut(s) 156, 199, 214, 366, 387, 1012, 1027
Cfr9I CCCGGG 1 cut(s) 153
CseI GACGC 4 cut(s) 116, 160, 289, 363
Csp6I GTAC 3 cut(s) 657, 698, 1189
CviAII CATG 7 cut(s) 100, 456, 671, 847, 862, 952, 1024
CviQI GTAC 3 cut(s) 657, 698, 1189
DdeI CTNAG 5 cut(s) 258, 279, 345, 550, 629
DpnI GATC 2 cut(s) 13, 1090
DpnII GATC 2 cut(s) 11, 1088
EaeI YGGCCR 2 cut(s) 102, 792
Eam1104I CTCTTC 1 cut(s) 1136
EarI CTCTTC 1 cut(s) 1136
EciI GGCGGA 3 cut(s) 4, 127, 340
Ecl136II GAGCTC 2 cut(s) 415, 427
Eco130I CCWWGG 4 cut(s) 99, 369, 846, 1071
Eco24I GRGCYC 4 cut(s) 239, 264, 417, 429
Eco32I GATATC 1 cut(s) 597
Eco47I GGWCC 2 cut(s) 387, 1012
Eco53kI GAGCTC 2 cut(s) 415, 427
Eco57I CTGAAG 2 cut(s) 507, 722
Eco88I CYCGRG 1 cut(s) 153
EcoICRI GAGCTC 2 cut(s) 415, 427
EcoRII CCWGG 3 cut(s) 195, 201, 263
EcoRV GATATC 1 cut(s) 597
EcoT14I CCWWGG 4 cut(s) 99, 369, 846, 1071
EcoT38I GRGCYC 4 cut(s) 239, 264, 417, 429
ErhI CCWWGG 4 cut(s) 99, 369, 846, 1071
FaeI CATG 7 cut(s) 103, 459, 674, 850, 865, 955, 1027
FalI AAGNNNNNCTT 2 cut(s) 713, 745
FaqI GGGAC 1 cut(s) 24
FatI CATG 7 cut(s) 99, 455, 670, 846, 861, 951, 1023
Fnu4HI GCNGC 9 cut(s) 111, 159, 207, 249, 273, 332, 357, 711, 975
FokI GGATG 2 cut(s) 863, 1049
FriOI GRGCYC 4 cut(s) 239, 264, 417, 429
Fsp4HI GCNGC 9 cut(s) 111, 159, 207, 249, 273, 332, 357, 711, 975
FspBI CTAG 3 cut(s) 530, 681, 692
GluI GCNGC 9 cut(s) 111, 159, 207, 249, 273, 332, 357, 711, 975
GsaI CCCAGC 1 cut(s) 489
GsuI CTGGAG 1 cut(s) 49
HapII CCGG 3 cut(s) 154, 269, 285
HgaI GACGC 4 cut(s) 116, 160, 289, 363
Hin1I GRCGYC 6 cut(s) 51, 108, 171, 243, 374, 467
Hin1II CATG 7 cut(s) 103, 459, 674, 850, 865, 955, 1027
HincII GTYRAC 1 cut(s) 859
HindII GTYRAC 1 cut(s) 859
HindIII AAGCTT 1 cut(s) 797
HinfI GANTC 8 cut(s) 74, 281, 361, 443, 615, 684, 827, 1180
HpaII CCGG 3 cut(s) 154, 269, 285
HphI GGTGA 1 cut(s) 169
Hpy166II GTNNAC 1 cut(s) 859
Hpy188I TCNGA 3 cut(s) 241, 280, 442
Hpy188III TCNNGA 7 cut(s) 29, 66, 430, 530, 635, 681, 692
Hpy8I GTNNAC 1 cut(s) 859
Hpy99I CGWCG 8 cut(s) 53, 56, 110, 176, 230, 245, 248, 469
HpyAV CCTTC 2 cut(s) 31, 427
HpyCH4III ACNGT 2 cut(s) 38, 777
HpyCH4IV ACGT 3 cut(s) 51, 243, 467
HpyCH4V TGCA 4 cut(s) 744, 833, 935, 1034
HpyF3I CTNAG 5 cut(s) 258, 279, 345, 550, 629
HpySE526I ACGT 3 cut(s) 51, 243, 467
Hsp92I GRCGYC 6 cut(s) 51, 108, 171, 243, 374, 467
Hsp92II CATG 7 cut(s) 103, 459, 674, 850, 865, 955, 1027
KroI GCCGGC 1 cut(s) 268
KroNI GCCGGC 1 cut(s) 270
Kzo9I GATC 2 cut(s) 11, 1088
LmnI GCTCC 3 cut(s) 242, 412, 822
Lsp1109I GCAGC 2 cut(s) 343, 961
MaeI CTAG 3 cut(s) 530, 681, 692
MaeII ACGT 3 cut(s) 51, 243, 467
MaeIII GTNAC 2 cut(s) 175, 511
MalI GATC 2 cut(s) 13, 1090
MboI GATC 2 cut(s) 11, 1088
MboII GAAGA 3 cut(s) 715, 765, 1123
MflI RGATCY 2 cut(s) 11, 1088
MhlI GDGCHC 5 cut(s) 239, 264, 417, 429, 1152
MlsI TGGCCA 1 cut(s) 794
MluCI AATT 2 cut(s) 420, 930
MluNI TGGCCA 1 cut(s) 794
MlyI GAGTC 3 cut(s) 68, 290, 693
MmeI TCCRAC 2 cut(s) 264, 990
Mox20I TGGCCA 1 cut(s) 794
MroNI GCCGGC 1 cut(s) 268
MroXI GAANNNNTTC 1 cut(s) 293
MscI TGGCCA 1 cut(s) 794
MseI TTAA 2 cut(s) 315, 450
MslI CAYNNNNRTG 2 cut(s) 404, 407
Msp20I TGGCCA 1 cut(s) 794
MspA1I CMGCKG 1 cut(s) 1186
MspCI CTTAAG 1 cut(s) 449
MspI CCGG 3 cut(s) 154, 269, 285
MspR9I CCNGG 6 cut(s) 154, 155, 197, 203, 265, 286
MvaI CCWGG 3 cut(s) 197, 203, 265
NaeI GCCGGC 1 cut(s) 270
NciI CCSGG 3 cut(s) 154, 155, 286
NcoI CCATGG 2 cut(s) 99, 846
NdeII GATC 2 cut(s) 11, 1088
NgoMIV GCCGGC 1 cut(s) 268
NlaIII CATG 7 cut(s) 103, 459, 674, 850, 865, 955, 1027
NlaIV GGNNCC 3 cut(s) 238, 1029, 1059
NmeAIII GCCGAG 1 cut(s) 277
NmuCI GTSAC 1 cut(s) 175
PcsI WCGNNNNNNNCGW 2 cut(s) 180, 249
PdiI GCCGGC 1 cut(s) 270
PdmI GAANNNNTTC 1 cut(s) 293
PfeI GAWTC 5 cut(s) 361, 443, 615, 827, 1180
PflMI CCANNNNNTGG 1 cut(s) 186
PfoI TCCNGGA 1 cut(s) 284
PkrI GCNGC 9 cut(s) 112, 160, 208, 250, 274, 333, 358, 712, 976
PleI GAGTC 3 cut(s) 68, 289, 692
PpsI GAGTC 3 cut(s) 68, 289, 692
PshAI GACNNNNGTC 1 cut(s) 36
PsiI TTATAA 2 cut(s) 383, 908
Psp124BI GAGCTC 2 cut(s) 417, 429
Psp6I CCWGG 3 cut(s) 195, 201, 263
PspFI CCCAGC 1 cut(s) 485
PspGI CCWGG 3 cut(s) 195, 201, 263
PspN4I GGNNCC 3 cut(s) 238, 1029, 1059
PspPI GGNCC 7 cut(s) 156, 199, 214, 366, 387, 1012, 1027
PstI CTGCAG 1 cut(s) 835
PstNI CAGNNNCTG 1 cut(s) 186
PsuI RGATCY 2 cut(s) 11, 1088
PvuII CAGCTG 1 cut(s) 1186
RsaI GTAC 3 cut(s) 658, 699, 1190
RsaNI GTAC 3 cut(s) 657, 698, 1189
RseI CAYNNNNRTG 2 cut(s) 404, 407
SacI GAGCTC 2 cut(s) 417, 429
SaqAI TTAA 2 cut(s) 315, 450
SatI GCNGC 9 cut(s) 111, 159, 207, 249, 273, 332, 357, 711, 975
Sau3AI GATC 2 cut(s) 11, 1088
Sau96I GGNCC 7 cut(s) 156, 199, 214, 366, 387, 1012, 1027
ScaI AGTACT 1 cut(s) 699
SchI GAGTC 3 cut(s) 68, 290, 693
ScrFI CCNGG 6 cut(s) 154, 155, 197, 203, 265, 286
SduI GDGCHC 5 cut(s) 239, 264, 417, 429, 1152
SfcI CTRYAG 1 cut(s) 831
SinI GGWCC 2 cut(s) 387, 1012
SmaI CCCGGG 1 cut(s) 155
SmiMI CAYNNNNRTG 2 cut(s) 404, 407
SmlI CTYRAG 2 cut(s) 428, 449
SmoI CTYRAG 2 cut(s) 428, 449
Sse9I AATT 2 cut(s) 420, 930
SspMI CTAG 3 cut(s) 530, 681, 692
SstI GAGCTC 2 cut(s) 417, 429
StyD4I CCNGG 6 cut(s) 152, 153, 195, 201, 263, 284
StyI CCWWGG 4 cut(s) 99, 369, 846, 1071
TaaI ACNGT 2 cut(s) 38, 777
TaiI ACGT 3 cut(s) 54, 246, 470
TaqI TCGA 4 cut(s) 30, 417, 517, 733
TasI AATT 2 cut(s) 420, 930
TatI WGTACW 2 cut(s) 697, 1188
TauI GCSGC 7 cut(s) 113, 161, 209, 251, 275, 359, 713
TfiI GAWTC 5 cut(s) 361, 443, 615, 827, 1180
Tru1I TTAA 2 cut(s) 315, 450
Tru9I TTAA 2 cut(s) 315, 450
TscAI CASTG 2 cut(s) 840, 1174
TseFI GTSAC 1 cut(s) 175
TseI GCWGC 2 cut(s) 331, 974
Tsp45I GTSAC 1 cut(s) 175
TspDTI ATGAA 3 cut(s) 13, 659, 968
TspGWI ACGGA 1 cut(s) 996
TspMI CCCGGG 1 cut(s) 153
TspRI CASTG 2 cut(s) 840, 1174
Van91I CCANNNNNTGG 1 cut(s) 186
Vha464I CTTAAG 1 cut(s) 449
VpaK11BI GGWCC 2 cut(s) 387, 1012
XbaI TCTAGA 3 cut(s) 529, 680, 691
XcmI CCANNNNNNNNNTGG 1 cut(s) 406
XmaI CCCGGG 1 cut(s) 153
XmnI GAANNNNTTC 1 cut(s) 293
XspI CTAG 3 cut(s) 530, 681, 692
ZraI GACGTC 3 cut(s) 52, 244, 468
ZrmI AGTACT 1 cut(s) 699
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.