MD09G1173400.v1.1

Belongs to the eIF-2B alpha beta delta subunits family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0006573
Physical Location & Seq
Forward (+)
14695059 .. 14697970
2912 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1173400.v1.1.491

Sequence Viewer

Length: 1209 bp
ATGTGGTGGGGATCAGCCTCTTTCATTCTCGACAAGCAGGACGAGGAGCGAAACGCCGCCGCCTTAGCCTCCAAACCTGAGTCCCTTAATCTCTCCCCGCCACCGCCGTCATCATCCATGGCCGACGCTCTGCAAAGCCCTAACCCTAAGATTTCGGCCTATTACCAGACGCGGGCGGCCCACCACGGCGTCGTCACAAGCGACTGGCTGGCCCAGGCCCAGGCCGCAGTCGGCAGAAACCCCGACGAGCAAGGGCCAATCACCGCTGAGGTTGAAGCTAAGCCGGGGTCTAATTCCGGCAAGCCCTTCAGCGTCATCGAGGAGTTCAATAGCTGGCGAAAGCAGCCCGATTTGGCGGAGGCCGTGGCGGCGATTCGGGCCTTGGCGTCTGTGATTCGGTCCAGTGAGGCCACCACTATGATGGAGCTCGAAATTGAGCTCAAGAAGGCCTCCGAGTCTTTGAAATCATGGGACACGACCTCTATTTCTTTGACGGCTGGATGTGACCTGTTCATTCGGTATGTTACTCGAACTTCAGCTTTAGAGTACGAGGACTTCAACTCAGCCAAGTCTCGTTTAATTGAACGGGCAGAGAAGTTTGGGGAGATATCGATGAAGGCCCGCAGAATCATTGCTGTGCTTAGTCAAGACTTTATATTTGATGGTTGCACCATCTTGGTTCATGGTTTCTCTAGAGTTGTTCTAGAAGTATTAAAAACTGCGGCACAGAGCAACAAACTCTTTCGAGTTTTCTGCACAGAGGGAAGACCAGACAGGACAGGATTACGATTATCGAATGAGCTGGCCAAGCTTGATGTTCCCGTGAAGCTTCTAATAGACTCTGCAGCAGCATATACCATGGATGAGGTTGACATGGTGTTTGTCGGGGCAGATGGAGTGGTTGAAAGTGGAGGTATCATCAATATGATGGGAACATTTCAAATTGCCTTAGTGGCACACAGCATGAACAAACCGGTTTATGTGGCTGCTGAAAGCTACAAGTTTGCTCGCCTTTATCCATTGGACCAGAAAGACATGGCCCCTGCATTACGTCCCATAGATTTTGGGGTACCCATCCCATCCAAGGTTGAAGTTGAAAAGTCTGCCCGTGATTATACTCCTCCGCAATATCTTACTCTGCTTTTCACGGATCTGGGCGTGCTAACTCCTTCAGTGGTCAGCGATGAGCTTATTCAGCTATACTTGTGA

Protein Analysis

403

Amino Acids

44.1

Weight (kDa)

5.14

Isoelectric Point (pI)

40.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
IF-2B PF01008 115 - 390 4.3e-71 Initiation factor 2 subunit family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1069
AccB1I GGYRCC 1 cut(s) 1069
AccII CGCG 1 cut(s) 172
AclWI GGATC 2 cut(s) 19, 1158
AcoI YGGCCR 2 cut(s) 120, 804
AcuI CTGAAG 3 cut(s) 292, 519, 1155
AcyI GRCGYC 2 cut(s) 189, 386
AfaI GTAC 2 cut(s) 548, 1071
AfiI CCNNNNNNNGG 3 cut(s) 172, 220, 1084
AgeI ACCGGT 1 cut(s) 973
AgsI TTSAA 9 cut(s) 275, 328, 463, 559, 584, 905, 941, 1091, 1097
AjnI CCWGG 2 cut(s) 213, 219
Alw21I GWGCWC 2 cut(s) 429, 441
Alw26I GTCTC 1 cut(s) 576
AlwI GGATC 2 cut(s) 19, 1158
ApeKI GCWGC 4 cut(s) 343, 845, 848, 986
ArsI GACNNNNNNTTYG 4 cut(s) 641, 673, 863, 895
AsiGI ACCGGT 1 cut(s) 973
Asp718I GGTACC 1 cut(s) 1069
AspS9I GGNCC 9 cut(s) 178, 211, 217, 254, 378, 399, 619, 1024, 1039
AsuC2I CCSGG 1 cut(s) 285
AsuHPI GGTGA 1 cut(s) 253
AvaII GGWCC 2 cut(s) 399, 1024
BalI TGGCCA 1 cut(s) 806
BanI GGYRCC 1 cut(s) 1069
BanII GRGCYC 2 cut(s) 429, 441
BarI GAAGNNNNNNTAC 2 cut(s) 539, 571
BbsI GAAGAC 1 cut(s) 772
Bbv12I GWGCWC 2 cut(s) 429, 441
BbvCI CCTCAGC 1 cut(s) 267
BbvI GCAGC 4 cut(s) 355, 857, 860, 973
BccI CCATC 7 cut(s) 415, 656, 680, 887, 922, 1082, 1087
BceAI ACGGC 4 cut(s) 91, 202, 347, 510
BcgI CGANNNNNNTGC 2 cut(s) 735, 769
BciT130I CCWGG 2 cut(s) 215, 221
BcnI CCSGG 1 cut(s) 285
BcoDI GTCTC 1 cut(s) 576
BfaI CTAG 2 cut(s) 693, 704
BfmI CTRYAG 1 cut(s) 843
BglI GCCNNNNNGGC 2 cut(s) 368, 953
BlpI GCTNAGC 1 cut(s) 279
Bme1390I CCNGG 3 cut(s) 215, 221, 285
Bme18I GGWCC 2 cut(s) 399, 1024
BmgT120I GGNCC 9 cut(s) 178, 211, 217, 254, 378, 399, 619, 1024, 1039
BmiI GGNNCC 2 cut(s) 1041, 1071
BmrFI CCNGG 3 cut(s) 215, 221, 285
BpiI GAAGAC 1 cut(s) 772
Bpu10I CCTNAGC 2 cut(s) 64, 267
Bpu1102I GCTNAGC 1 cut(s) 279
BpuEI CTTGAG 1 cut(s) 425
BpuMI CCSGG 1 cut(s) 285
Bsa29I ATCGAT 1 cut(s) 611
BsaHI GRCGYC 2 cut(s) 189, 386
BsaJI CCNNGG 9 cut(s) 117, 184, 213, 219, 284, 363, 381, 858, 1083
BsaWI WCCGGW 1 cut(s) 973
Bsc4I CCNNNNNNNGG 3 cut(s) 172, 220, 1084
Bse118I RCCGGY 1 cut(s) 973
Bse1I ACTGG 2 cut(s) 209, 402
Bse3DI GCAATG 1 cut(s) 630
BseBI CCWGG 2 cut(s) 215, 221
BseCI ATCGAT 1 cut(s) 611
BseDI CCNNGG 9 cut(s) 117, 184, 213, 219, 284, 363, 381, 858, 1083
BseGI GGATG 5 cut(s) 113, 506, 868, 1074, 1079
BseLI CCNNNNNNNGG 3 cut(s) 172, 220, 1084
BseMI GCAATG 1 cut(s) 630
BseMII CTCAG 3 cut(s) 69, 258, 576
BseNI ACTGG 2 cut(s) 209, 402
BseRI GAGGAG 3 cut(s) 59, 335, 1110
BseXI GCAGC 4 cut(s) 355, 857, 860, 973
BsgI GTGCAG 1 cut(s) 739
Bsh1236I CGCG 1 cut(s) 172
BshNI GGYRCC 1 cut(s) 1069
BshTI ACCGGT 1 cut(s) 973
BshVI ATCGAT 1 cut(s) 611
BsiHKAI GWGCWC 2 cut(s) 429, 441
BsiSI CCGG 3 cut(s) 284, 297, 974
BslFI GGGAC 3 cut(s) 67, 485, 1038
BslI CCNNNNNNNGG 3 cut(s) 172, 220, 1084
BsmAI GTCTC 1 cut(s) 576
BsmFI GGGAC 3 cut(s) 67, 485, 1038
Bsp1286I GDGCHC 2 cut(s) 429, 441
Bsp143I GATC 2 cut(s) 11, 1150
Bsp1720I GCTNAGC 1 cut(s) 279
Bsp19I CCATGG 2 cut(s) 117, 858
BspCNI CTCAG 3 cut(s) 70, 259, 575
BspDI ATCGAT 1 cut(s) 611
BspFNI CGCG 1 cut(s) 172
BspLI GGNNCC 2 cut(s) 1041, 1071
BspMAI CTGCAG 1 cut(s) 847
BspPI GGATC 2 cut(s) 19, 1158
BspT107I GGYRCC 1 cut(s) 1069
BsrDI GCAATG 1 cut(s) 630
BsrFI RCCGGY 1 cut(s) 973
BsrI ACTGG 2 cut(s) 209, 402
BssAI RCCGGY 1 cut(s) 973
BssECI CCNNGG 9 cut(s) 117, 184, 213, 219, 284, 363, 381, 858, 1083
BssMI GATC 2 cut(s) 11, 1150
BssNI GRCGYC 2 cut(s) 189, 386
BssT1I CCWWGG 4 cut(s) 117, 381, 858, 1083
Bst2UI CCWGG 2 cut(s) 215, 221
BstACI GRCGYC 2 cut(s) 189, 386
BstC8I GCNNGC 8 cut(s) 174, 210, 302, 335, 622, 804, 1009, 1160
BstDEI CTNAG 8 cut(s) 64, 78, 147, 267, 279, 562, 641, 949
BstDSI CCRYGG 4 cut(s) 117, 184, 363, 858
BstF5I GGATG 5 cut(s) 113, 506, 868, 1074, 1079
BstFNI CGCG 1 cut(s) 172
BstKTI GATC 2 cut(s) 14, 1153
BstMAI GTCTC 1 cut(s) 576
BstMBI GATC 2 cut(s) 11, 1150
BstMWI GCNNNNNNNGC 8 cut(s) 65, 224, 343, 368, 377, 808, 953, 1195
BstNI CCWGG 2 cut(s) 215, 221
BstSCI CCNGG 3 cut(s) 213, 219, 283
BstSFI CTRYAG 1 cut(s) 843
BstUI CGCG 1 cut(s) 172
BstV1I GCAGC 4 cut(s) 355, 857, 860, 973
BstV2I GAAGAC 1 cut(s) 772
BstX2I RGATCY 1 cut(s) 1150
BstXI CCANNNNNNTGG 1 cut(s) 421
BstYI RGATCY 1 cut(s) 1150
Bsu15I ATCGAT 1 cut(s) 611
BsuTUI ATCGAT 1 cut(s) 611
BtgI CCRYGG 4 cut(s) 117, 184, 363, 858
BtgZI GCGATG 1 cut(s) 1197
BtsCI GGATG 5 cut(s) 113, 506, 868, 1074, 1079
BtsIMutI CAGTG 2 cut(s) 409, 1179
Cac8I GCNNGC 8 cut(s) 174, 210, 302, 335, 622, 804, 1009, 1160
Cfr10I RCCGGY 1 cut(s) 973
Cfr13I GGNCC 9 cut(s) 178, 211, 217, 254, 378, 399, 619, 1024, 1039
ClaI ATCGAT 1 cut(s) 611
CseI GACGC 5 cut(s) 134, 178, 178, 301, 375
Csp6I GTAC 2 cut(s) 547, 1070
CspAI ACCGGT 1 cut(s) 973
CviAII CATG 7 cut(s) 118, 468, 683, 859, 874, 964, 1036
CviQI GTAC 2 cut(s) 547, 1070
DdeI CTNAG 8 cut(s) 64, 78, 147, 267, 279, 562, 641, 949
DpnI GATC 2 cut(s) 13, 1152
DpnII GATC 2 cut(s) 11, 1150
EaeI YGGCCR 2 cut(s) 120, 804
EciI GGCGGA 1 cut(s) 371
Ecl136II GAGCTC 2 cut(s) 427, 439
Eco130I CCWWGG 4 cut(s) 117, 381, 858, 1083
Eco147I AGGCCT 1 cut(s) 449
Eco24I GRGCYC 2 cut(s) 429, 441
Eco32I GATATC 1 cut(s) 609
Eco47I GGWCC 2 cut(s) 399, 1024
Eco53kI GAGCTC 2 cut(s) 427, 439
Eco57I CTGAAG 3 cut(s) 292, 519, 1155
EcoICRI GAGCTC 2 cut(s) 427, 439
EcoRII CCWGG 2 cut(s) 213, 219
EcoRV GATATC 1 cut(s) 609
EcoT14I CCWWGG 4 cut(s) 117, 381, 858, 1083
EcoT38I GRGCYC 2 cut(s) 429, 441
ErhI CCWWGG 4 cut(s) 117, 381, 858, 1083
FaeI CATG 7 cut(s) 121, 471, 686, 862, 877, 967, 1039
FaqI GGGAC 3 cut(s) 67, 485, 1038
FatI CATG 7 cut(s) 117, 467, 682, 858, 873, 963, 1035
FauI CCCGC 3 cut(s) 105, 165, 629
FokI GGATG 5 cut(s) 100, 513, 875, 1061, 1066
FriOI GRGCYC 2 cut(s) 429, 441
FspBI CTAG 2 cut(s) 693, 704
HapII CCGG 3 cut(s) 284, 297, 974
HgaI GACGC 5 cut(s) 134, 178, 178, 301, 375
Hin1I GRCGYC 2 cut(s) 189, 386
Hin1II CATG 7 cut(s) 121, 471, 686, 862, 877, 967, 1039
HincII GTYRAC 1 cut(s) 871
HindII GTYRAC 1 cut(s) 871
HindIII AAGCTT 2 cut(s) 809, 827
HinfI GANTC 6 cut(s) 80, 373, 394, 455, 627, 839
HpaII CCGG 3 cut(s) 284, 297, 974
HphI GGTGA 1 cut(s) 253
Hpy166II GTNNAC 1 cut(s) 871
Hpy188I TCNGA 1 cut(s) 454
Hpy188III TCNNGA 5 cut(s) 29, 442, 647, 693, 704
Hpy8I GTNNAC 1 cut(s) 871
Hpy99I CGWCG 3 cut(s) 128, 194, 248
HpyAV CCTTC 4 cut(s) 316, 439, 610, 1179
HpyCH4IV ACGT 1 cut(s) 1051
HpyCH4V TGCA 5 cut(s) 133, 669, 756, 845, 1046
HpyF10VI GCNNNNNNNGC 8 cut(s) 65, 224, 343, 368, 377, 808, 953, 1195
HpyF3I CTNAG 8 cut(s) 64, 78, 147, 267, 279, 562, 641, 949
HpySE526I ACGT 1 cut(s) 1051
Hsp92I GRCGYC 2 cut(s) 189, 386
Hsp92II CATG 7 cut(s) 121, 471, 686, 862, 877, 967, 1039
KpnI GGTACC 1 cut(s) 1073
Kzo9I GATC 2 cut(s) 11, 1150
LmnI GCTCC 2 cut(s) 46, 424
Lsp1109I GCAGC 4 cut(s) 355, 857, 860, 973
MaeI CTAG 2 cut(s) 693, 704
MaeII ACGT 1 cut(s) 1051
MaeIII GTNAC 3 cut(s) 193, 503, 523
MalI GATC 2 cut(s) 13, 1152
MboI GATC 2 cut(s) 11, 1150
MboII GAAGA 1 cut(s) 777
MflI RGATCY 1 cut(s) 1150
MhlI GDGCHC 2 cut(s) 429, 441
MlsI TGGCCA 1 cut(s) 806
MluCI AATT 4 cut(s) 292, 432, 579, 942
MluNI TGGCCA 1 cut(s) 806
MlyI GAGTC 3 cut(s) 89, 464, 833
Mox20I TGGCCA 1 cut(s) 806
MscI TGGCCA 1 cut(s) 806
MseI TTAA 3 cut(s) 87, 578, 713
MslI CAYNNNNRTG 4 cut(s) 416, 419, 635, 923
Msp20I TGGCCA 1 cut(s) 806
MspA1I CMGCKG 1 cut(s) 266
MspI CCGG 3 cut(s) 284, 297, 974
MspR9I CCNGG 3 cut(s) 215, 221, 285
MvaI CCWGG 2 cut(s) 215, 221
MvnI CGCG 1 cut(s) 172
MwoI GCNNNNNNNGC 8 cut(s) 65, 224, 343, 368, 377, 808, 953, 1195
NciI CCSGG 1 cut(s) 285
NcoI CCATGG 2 cut(s) 117, 858
NdeII GATC 2 cut(s) 11, 1150
NlaIII CATG 7 cut(s) 121, 471, 686, 862, 877, 967, 1039
NlaIV GGNNCC 2 cut(s) 1041, 1071
NmuCI GTSAC 2 cut(s) 193, 503
PceI AGGCCT 1 cut(s) 449
PcsI WCGNNNNNNNCGW 1 cut(s) 198
PfeI GAWTC 3 cut(s) 373, 394, 627
PinAI ACCGGT 1 cut(s) 973
PleI GAGTC 3 cut(s) 88, 463, 833
PpsI GAGTC 3 cut(s) 88, 463, 833
Psp124BI GAGCTC 2 cut(s) 429, 441
Psp6I CCWGG 2 cut(s) 213, 219
PspGI CCWGG 2 cut(s) 213, 219
PspN4I GGNNCC 2 cut(s) 1041, 1071
PspPI GGNCC 9 cut(s) 178, 211, 217, 254, 378, 399, 619, 1024, 1039
PstI CTGCAG 1 cut(s) 847
PsuI RGATCY 1 cut(s) 1150
RsaI GTAC 2 cut(s) 548, 1071
RsaNI GTAC 2 cut(s) 547, 1070
RseI CAYNNNNRTG 4 cut(s) 416, 419, 635, 923
SacI GAGCTC 2 cut(s) 429, 441
SaqAI TTAA 3 cut(s) 87, 578, 713
Sau3AI GATC 2 cut(s) 11, 1150
Sau96I GGNCC 9 cut(s) 178, 211, 217, 254, 378, 399, 619, 1024, 1039
SchI GAGTC 3 cut(s) 89, 464, 833
ScrFI CCNGG 3 cut(s) 215, 221, 285
SduI GDGCHC 2 cut(s) 429, 441
SfcI CTRYAG 1 cut(s) 843
SinI GGWCC 2 cut(s) 399, 1024
SmiMI CAYNNNNRTG 4 cut(s) 416, 419, 635, 923
SmlI CTYRAG 1 cut(s) 440
SmoI CTYRAG 1 cut(s) 440
Sse9I AATT 4 cut(s) 292, 432, 579, 942
SseBI AGGCCT 1 cut(s) 449
SspMI CTAG 2 cut(s) 693, 704
SstI GAGCTC 2 cut(s) 429, 441
StuI AGGCCT 1 cut(s) 449
StyD4I CCNGG 3 cut(s) 213, 219, 283
StyI CCWWGG 4 cut(s) 117, 381, 858, 1083
TaiI ACGT 1 cut(s) 1054
TaqI TCGA 7 cut(s) 30, 318, 429, 529, 611, 745, 794
TaqII GACCGA 1 cut(s) 387
TasI AATT 4 cut(s) 292, 432, 579, 942
TauI GCSGC 6 cut(s) 59, 62, 179, 227, 371, 725
TfiI GAWTC 3 cut(s) 373, 394, 627
Tru1I TTAA 3 cut(s) 87, 578, 713
Tru9I TTAA 3 cut(s) 87, 578, 713
TscAI CASTG 2 cut(s) 409, 1179
TseFI GTSAC 2 cut(s) 193, 503
TseI GCWGC 4 cut(s) 343, 845, 848, 986
Tsp45I GTSAC 2 cut(s) 193, 503
TspDTI ATGAA 5 cut(s) 13, 502, 629, 671, 980
TspGWI ACGGA 1 cut(s) 1163
TspRI CASTG 2 cut(s) 409, 1179
VpaK11BI GGWCC 2 cut(s) 399, 1024
XbaI TCTAGA 2 cut(s) 692, 703
XcmI CCANNNNNNNNNTGG 1 cut(s) 418
XspI CTAG 2 cut(s) 693, 704
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.