RchiOBHm_Chr2g0147751

Belongs to the eIF-2B alpha beta delta subunits family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0006573
Physical Location & Seq
Reverse (-)
65319345 .. 65322277
2933 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ51735

Sequence Viewer

Length: 1203 bp
ATGTGGTGGAGATCCGCTTCCTTCATTCTCGACAACCGTCCCCAAAACGACGTCGCACCGCCCTCCAGACCCGACTCCCTTACTCTCTCCCCACCCACCATGGCCGACGCCGCCCTGCAAAGCCCTAACGCCAACTCCGCCTACTACCAGACCCGGGCCGCCCACCACGGCGTCGTCACCAGCGACTGGCTCGCCCAGGCCCAGGCCGCGGCCGCCGTGGGCCGAAACCCCGACGATCAGGGCTCCGTCGTCGCCGCCGAGGCTGAGCCCAAGCCGGCGGCTTCTGAGTCCGGGAAGCCGTTCAGCGTCATCGTGGAGTTTAATAACTGGCGGAAGCAGCCTGATTTGGCTGAGGCCGTGGCGGCGATTCGGGCCTTGGCGTCTGTTATTAGGTCCAGCGAGGCCACCACTATGATGGAGCTCGAAATTGAGCTCAAGAAGGCTTCCGATTCTCTTAAGTCATGGGATACGACGTCTATATCTTTGACTGCTGGATGCGACCTGTTTATGCGGTATGTTACTCGAACTTCAGCTCTAGAGTATGAGGATTTCCACTCAGCCAAGTCTCGTTTGATTGAACGGGCAGAGAAGTTTGGGGAGATATCTACAAAGGCTCGTAGAATCATTGCTGTCCTTAGTCAAGACTTTATATTTGATGGTTGTACCATCTTGGTTCATGGTTTCTCTAGAGTCGTTCTAGAAGTACTGAAGACTGCGGCACAGAGCAAGAAACTCTTTCGAGTTTTCTGCACAGAGGGAAGACCAGACAGAACAGGATTACGGTTATCAAATGAGCTGGCCAAGCTTGATGTTCCTGTGAAGCTCCTAATAGATTCTGCAGTGGCATATACCATGGATGAGGTTGACATGGTGTTTGTTGGGGCAGATGGAGTGGTTGAAAGTGGAGGTATTATAAATATGATGGGAACATATCAAATTGCATTGGTGGCACATAGCATGAACAAACCAGTTTATGTGGCTGCTGAAAGCTACAAGTTCGCTCGCCTTTATCCATTGGACCAGAAAGACATGGCCCCTGCATTACGCCCCATTGATTTTGGGGTTCCCATCCCGTCCAAGGTTGAGGTTGAAAGATCTGCCCGTGATTATACGCCTCCTCAATATCTCACTTTACTCTTCACAGACTTGGGTGTGCTCACTCCATCGGTTGTCAGTGATGAGCTGATTCAGCTGTACTTGTAA

Protein Analysis

400

Amino Acids

43.9

Weight (kDa)

5.34

Isoelectric Point (pI)

39.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
IF-2B PF01008 113 - 388 2.6e-72 Initiation factor 2 subunit family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 914
AatII GACGTC 2 cut(s) 54, 476
AccB7I CCANNNNNTGG 1 cut(s) 186
AccII CGCG 1 cut(s) 209
AclWI GGATC 1 cut(s) 6
AcoI YGGCCR 3 cut(s) 102, 210, 798
AcuI CTGAAG 2 cut(s) 513, 728
AcyI GRCGYC 5 cut(s) 51, 108, 171, 380, 473
AfaI GTAC 3 cut(s) 664, 705, 1196
AfiI CCNNNNNNNGG 5 cut(s) 154, 186, 202, 208, 1078
AflII CTTAAG 1 cut(s) 455
AgsI TTSAA 3 cut(s) 578, 899, 1091
AjnI CCWGG 2 cut(s) 195, 201
AluBI AGCT 9 cut(s) 421, 433, 533, 796, 805, 823, 990, 1183, 1192
AluI AGCT 9 cut(s) 421, 433, 533, 796, 805, 823, 990, 1183, 1192
Alw21I GWGCWC 3 cut(s) 423, 435, 1158
Alw26I GTCTC 1 cut(s) 570
AlwI GGATC 1 cut(s) 6
AlwNI CAGNNNCTG 1 cut(s) 186
Ama87I CYCGRG 1 cut(s) 153
ApeKI GCWGC 2 cut(s) 337, 980
ArsI GACNNNNNNTTYG 4 cut(s) 635, 667, 857, 889
Asp700I GAANNNNTTC 1 cut(s) 299
AspS9I GGNCC 7 cut(s) 156, 199, 220, 372, 393, 1018, 1033
AsuC2I CCSGG 3 cut(s) 154, 155, 292
AsuHPI GGTGA 1 cut(s) 169
AvaI CYCGRG 1 cut(s) 153
AvaII GGWCC 2 cut(s) 393, 1018
BalI TGGCCA 1 cut(s) 800
BanII GRGCYC 4 cut(s) 245, 270, 423, 435
BbsI GAAGAC 2 cut(s) 716, 766
Bbv12I GWGCWC 3 cut(s) 423, 435, 1158
BbvCI CCTCAGC 1 cut(s) 351
BbvI GCAGC 2 cut(s) 349, 967
BccI CCATC 7 cut(s) 409, 650, 674, 881, 916, 1076, 1171
BceAI ACGGC 4 cut(s) 184, 200, 283, 341
BcgI CGANNNNNNTGC 2 cut(s) 729, 763
BciT130I CCWGG 2 cut(s) 197, 203
BciVI GTATCC 1 cut(s) 460
BcnI CCSGG 3 cut(s) 154, 155, 292
BcoDI GTCTC 1 cut(s) 570
BfaI CTAG 3 cut(s) 536, 687, 698
BfmI CTRYAG 1 cut(s) 837
BfrI CTTAAG 1 cut(s) 455
BfuI GTATCC 1 cut(s) 460
BglI GCCNNNNNGGC 2 cut(s) 260, 362
BglII AGATCT 1 cut(s) 1094
BlpI GCTNAGC 1 cut(s) 264
BmcAI AGTACT 1 cut(s) 705
Bme1390I CCNGG 5 cut(s) 154, 155, 197, 203, 292
Bme18I GGWCC 2 cut(s) 393, 1018
BmeT110I CYCGRG 1 cut(s) 153
BmgT120I GGNCC 7 cut(s) 156, 199, 220, 372, 393, 1018, 1033
BmiI GGNNCC 3 cut(s) 244, 1035, 1065
BmrFI CCNGG 5 cut(s) 154, 155, 197, 203, 292
BmsI GCATC 1 cut(s) 485
BoxI GACNNNNGTC 1 cut(s) 36
BpiI GAAGAC 2 cut(s) 716, 766
BpmI CTGGAG 1 cut(s) 49
Bpu10I CCTNAGC 1 cut(s) 351
Bpu1102I GCTNAGC 1 cut(s) 264
BpuEI CTTGAG 1 cut(s) 419
BpuMI CCSGG 3 cut(s) 154, 155, 292
BsaHI GRCGYC 5 cut(s) 51, 108, 171, 380, 473
BsaXI ACNNNNNCTCC 2 cut(s) 119, 149
Bsc4I CCNNNNNNNGG 5 cut(s) 154, 186, 202, 208, 1078
Bse118I RCCGGY 1 cut(s) 274
Bse1I ACTGG 3 cut(s) 191, 332, 968
Bse3DI GCAATG 1 cut(s) 624
BseBI CCWGG 2 cut(s) 197, 203
BseGI GGATG 3 cut(s) 500, 862, 1068
BseLI CCNNNNNNNGG 5 cut(s) 154, 186, 202, 208, 1078
BseMI GCAATG 1 cut(s) 624
BseMII CTCAG 4 cut(s) 255, 276, 342, 570
BseNI ACTGG 3 cut(s) 191, 332, 968
BseRI GAGGAG 1 cut(s) 1107
BseX3I CGGCCG 1 cut(s) 210
BseXI GCAGC 2 cut(s) 349, 967
BsgI GTGCAG 1 cut(s) 733
Bsh1236I CGCG 1 cut(s) 209
Bsh1285I CGRYCG 1 cut(s) 213
BsiEI CGRYCG 1 cut(s) 213
BsiHKAI GWGCWC 3 cut(s) 423, 435, 1158
BsiHKCI CYCGRG 1 cut(s) 153
BsiSI CCGG 3 cut(s) 154, 275, 291
BslFI GGGAC 1 cut(s) 24
BslI CCNNNNNNNGG 5 cut(s) 154, 186, 202, 208, 1078
BsmAI GTCTC 1 cut(s) 570
BsmFI GGGAC 1 cut(s) 24
BsoBI CYCGRG 1 cut(s) 153
Bsp1286I GDGCHC 5 cut(s) 245, 270, 423, 435, 1158
Bsp143I GATC 3 cut(s) 11, 235, 1094
Bsp1720I GCTNAGC 1 cut(s) 264
Bsp19I CCATGG 2 cut(s) 99, 852
BspCNI CTCAG 4 cut(s) 256, 277, 343, 569
BspFNI CGCG 1 cut(s) 209
BspLI GGNNCC 3 cut(s) 244, 1035, 1065
BspMAI CTGCAG 1 cut(s) 841
BspPI GGATC 1 cut(s) 6
BspTI CTTAAG 1 cut(s) 455
BsrDI GCAATG 1 cut(s) 624
BsrFI RCCGGY 1 cut(s) 274
BsrI ACTGG 3 cut(s) 191, 332, 968
BssAI RCCGGY 1 cut(s) 274
BssMI GATC 3 cut(s) 11, 235, 1094
BssNI GRCGYC 5 cut(s) 51, 108, 171, 380, 473
BssT1I CCWWGG 4 cut(s) 99, 375, 852, 1077
Bst2UI CCWGG 2 cut(s) 197, 203
Bst4CI ACNGT 2 cut(s) 38, 783
Bst6I CTCTTC 1 cut(s) 1142
BstACI GRCGYC 5 cut(s) 51, 108, 171, 380, 473
BstAFI CTTAAG 1 cut(s) 455
BstC8I GCNNGC 4 cut(s) 192, 276, 798, 1003
BstDEI CTNAG 5 cut(s) 264, 285, 351, 556, 635
BstDSI CCRYGG 6 cut(s) 99, 166, 207, 216, 357, 852
BstF5I GGATG 3 cut(s) 500, 862, 1068
BstFNI CGCG 1 cut(s) 209
BstKTI GATC 3 cut(s) 14, 238, 1097
BstMAI GTCTC 1 cut(s) 570
BstMBI GATC 3 cut(s) 11, 235, 1094
BstMCI CGRYCG 1 cut(s) 213
BstNI CCWGG 2 cut(s) 197, 203
BstPAI GACNNNNGTC 1 cut(s) 36
BstSCI CCNGG 5 cut(s) 152, 153, 195, 201, 290
BstSFI CTRYAG 1 cut(s) 837
BstUI CGCG 1 cut(s) 209
BstV1I GCAGC 2 cut(s) 349, 967
BstV2I GAAGAC 2 cut(s) 716, 766
BstX2I RGATCY 2 cut(s) 11, 1094
BstXI CCANNNNNNTGG 1 cut(s) 415
BstYI RGATCY 2 cut(s) 11, 1094
BstZI CGGCCG 1 cut(s) 210
BsuI GTATCC 1 cut(s) 460
BtgI CCRYGG 6 cut(s) 99, 166, 207, 216, 357, 852
BtsCI GGATG 3 cut(s) 500, 862, 1068
BtsI GCAGTG 1 cut(s) 846
BtsIMutI CAGTG 2 cut(s) 846, 1180
Cac8I GCNNGC 4 cut(s) 192, 276, 798, 1003
CaiI CAGNNNCTG 1 cut(s) 186
CciNI GCGGCCGC 1 cut(s) 210
Cfr10I RCCGGY 1 cut(s) 274
Cfr13I GGNCC 7 cut(s) 156, 199, 220, 372, 393, 1018, 1033
Cfr42I CCGCGG 1 cut(s) 210
Cfr9I CCCGGG 1 cut(s) 153
CseI GACGC 4 cut(s) 116, 160, 295, 369
Csp6I GTAC 3 cut(s) 663, 704, 1195
CviAII CATG 7 cut(s) 100, 462, 677, 853, 868, 958, 1030
CviQI GTAC 3 cut(s) 663, 704, 1195
DdeI CTNAG 5 cut(s) 264, 285, 351, 556, 635
DpnI GATC 3 cut(s) 13, 237, 1096
DpnII GATC 3 cut(s) 11, 235, 1094
EaeI YGGCCR 3 cut(s) 102, 210, 798
EagI CGGCCG 1 cut(s) 210
Eam1104I CTCTTC 1 cut(s) 1142
EarI CTCTTC 1 cut(s) 1142
EciI GGCGGA 2 cut(s) 127, 346
Ecl136II GAGCTC 2 cut(s) 421, 433
EclXI CGGCCG 1 cut(s) 210
Eco130I CCWWGG 4 cut(s) 99, 375, 852, 1077
Eco24I GRGCYC 4 cut(s) 245, 270, 423, 435
Eco32I GATATC 1 cut(s) 603
Eco47I GGWCC 2 cut(s) 393, 1018
Eco52I CGGCCG 1 cut(s) 210
Eco53kI GAGCTC 2 cut(s) 421, 433
Eco57I CTGAAG 2 cut(s) 513, 728
Eco88I CYCGRG 1 cut(s) 153
EcoICRI GAGCTC 2 cut(s) 421, 433
EcoRII CCWGG 2 cut(s) 195, 201
EcoRV GATATC 1 cut(s) 603
EcoT14I CCWWGG 4 cut(s) 99, 375, 852, 1077
EcoT38I GRGCYC 4 cut(s) 245, 270, 423, 435
ErhI CCWWGG 4 cut(s) 99, 375, 852, 1077
FaeI CATG 7 cut(s) 103, 465, 680, 856, 871, 961, 1033
FalI AAGNNNNNCTT 2 cut(s) 719, 751
FaqI GGGAC 1 cut(s) 24
FatI CATG 7 cut(s) 99, 461, 676, 852, 867, 957, 1029
FokI GGATG 3 cut(s) 507, 869, 1055
FriOI GRGCYC 4 cut(s) 245, 270, 423, 435
FspBI CTAG 3 cut(s) 536, 687, 698
GsuI CTGGAG 1 cut(s) 49
HapII CCGG 3 cut(s) 154, 275, 291
HgaI GACGC 4 cut(s) 116, 160, 295, 369
Hin1I GRCGYC 5 cut(s) 51, 108, 171, 380, 473
Hin1II CATG 7 cut(s) 103, 465, 680, 856, 871, 961, 1033
HincII GTYRAC 1 cut(s) 865
HindII GTYRAC 1 cut(s) 865
HindIII AAGCTT 1 cut(s) 803
HinfI GANTC 8 cut(s) 74, 287, 367, 449, 621, 690, 833, 1186
HpaII CCGG 3 cut(s) 154, 275, 291
HphI GGTGA 1 cut(s) 169
Hpy166II GTNNAC 1 cut(s) 865
Hpy188I TCNGA 2 cut(s) 286, 448
Hpy188III TCNNGA 7 cut(s) 29, 66, 436, 536, 641, 687, 698
Hpy8I GTNNAC 1 cut(s) 865
Hpy99I CGWCG 8 cut(s) 53, 56, 110, 176, 236, 251, 254, 475
HpyAV CCTTC 2 cut(s) 31, 433
HpyCH4III ACNGT 2 cut(s) 38, 783
HpyCH4IV ACGT 2 cut(s) 51, 473
HpyCH4V TGCA 5 cut(s) 118, 750, 839, 941, 1040
HpyF3I CTNAG 5 cut(s) 264, 285, 351, 556, 635
HpySE526I ACGT 2 cut(s) 51, 473
Hsp92I GRCGYC 5 cut(s) 51, 108, 171, 380, 473
Hsp92II CATG 7 cut(s) 103, 465, 680, 856, 871, 961, 1033
KroI GCCGGC 1 cut(s) 274
KroNI GCCGGC 1 cut(s) 276
KspI CCGCGG 1 cut(s) 210
Kzo9I GATC 3 cut(s) 11, 235, 1094
LmnI GCTCC 3 cut(s) 248, 418, 828
Lsp1109I GCAGC 2 cut(s) 349, 967
LweI GCATC 1 cut(s) 485
MaeI CTAG 3 cut(s) 536, 687, 698
MaeII ACGT 2 cut(s) 51, 473
MaeIII GTNAC 2 cut(s) 175, 517
MalI GATC 3 cut(s) 13, 237, 1096
MboI GATC 3 cut(s) 11, 235, 1094
MboII GAAGA 3 cut(s) 721, 771, 1129
MflI RGATCY 2 cut(s) 11, 1094
MhlI GDGCHC 5 cut(s) 245, 270, 423, 435, 1158
MlsI TGGCCA 1 cut(s) 800
MluCI AATT 2 cut(s) 426, 936
MluNI TGGCCA 1 cut(s) 800
MlyI GAGTC 3 cut(s) 68, 296, 699
Mox20I TGGCCA 1 cut(s) 800
MroNI GCCGGC 1 cut(s) 274
MroXI GAANNNNTTC 1 cut(s) 299
MscI TGGCCA 1 cut(s) 800
MseI TTAA 2 cut(s) 321, 456
MslI CAYNNNNRTG 2 cut(s) 410, 413
Msp20I TGGCCA 1 cut(s) 800
MspA1I CMGCKG 2 cut(s) 209, 1192
MspCI CTTAAG 1 cut(s) 455
MspI CCGG 3 cut(s) 154, 275, 291
MspR9I CCNGG 5 cut(s) 154, 155, 197, 203, 292
MvaI CCWGG 2 cut(s) 197, 203
MvnI CGCG 1 cut(s) 209
NaeI GCCGGC 1 cut(s) 276
NciI CCSGG 3 cut(s) 154, 155, 292
NcoI CCATGG 2 cut(s) 99, 852
NdeII GATC 3 cut(s) 11, 235, 1094
NgoMIV GCCGGC 1 cut(s) 274
NlaIII CATG 7 cut(s) 103, 465, 680, 856, 871, 961, 1033
NlaIV GGNNCC 3 cut(s) 244, 1035, 1065
NmeAIII GCCGAG 1 cut(s) 283
NmuCI GTSAC 1 cut(s) 175
NotI GCGGCCGC 1 cut(s) 210
PcsI WCGNNNNNNNCGW 2 cut(s) 180, 255
PdiI GCCGGC 1 cut(s) 276
PdmI GAANNNNTTC 1 cut(s) 299
PfeI GAWTC 5 cut(s) 367, 449, 621, 833, 1186
PflMI CCANNNNNTGG 1 cut(s) 186
PfoI TCCNGGA 1 cut(s) 290
PleI GAGTC 3 cut(s) 68, 295, 698
PpsI GAGTC 3 cut(s) 68, 295, 698
PshAI GACNNNNGTC 1 cut(s) 36
PsiI TTATAA 1 cut(s) 914
Psp124BI GAGCTC 2 cut(s) 423, 435
Psp6I CCWGG 2 cut(s) 195, 201
PspGI CCWGG 2 cut(s) 195, 201
PspN4I GGNNCC 3 cut(s) 244, 1035, 1065
PspPI GGNCC 7 cut(s) 156, 199, 220, 372, 393, 1018, 1033
PstI CTGCAG 1 cut(s) 841
PstNI CAGNNNCTG 1 cut(s) 186
PsuI RGATCY 2 cut(s) 11, 1094
PvuII CAGCTG 1 cut(s) 1192
RsaI GTAC 3 cut(s) 664, 705, 1196
RsaNI GTAC 3 cut(s) 663, 704, 1195
RseI CAYNNNNRTG 2 cut(s) 410, 413
SacI GAGCTC 2 cut(s) 423, 435
SacII CCGCGG 1 cut(s) 210
SaqAI TTAA 2 cut(s) 321, 456
Sau3AI GATC 3 cut(s) 11, 235, 1094
Sau96I GGNCC 7 cut(s) 156, 199, 220, 372, 393, 1018, 1033
ScaI AGTACT 1 cut(s) 705
SchI GAGTC 3 cut(s) 68, 296, 699
ScrFI CCNGG 5 cut(s) 154, 155, 197, 203, 292
SduI GDGCHC 5 cut(s) 245, 270, 423, 435, 1158
SfaNI GCATC 1 cut(s) 485
SfcI CTRYAG 1 cut(s) 837
Sfr303I CCGCGG 1 cut(s) 210
SgrBI CCGCGG 1 cut(s) 210
SinI GGWCC 2 cut(s) 393, 1018
SmaI CCCGGG 1 cut(s) 155
SmiMI CAYNNNNRTG 2 cut(s) 410, 413
SmlI CTYRAG 2 cut(s) 434, 455
SmoI CTYRAG 2 cut(s) 434, 455
Sse9I AATT 2 cut(s) 426, 936
SspMI CTAG 3 cut(s) 536, 687, 698
SstI GAGCTC 2 cut(s) 423, 435
StyD4I CCNGG 5 cut(s) 152, 153, 195, 201, 290
StyI CCWWGG 4 cut(s) 99, 375, 852, 1077
TaaI ACNGT 2 cut(s) 38, 783
TaiI ACGT 2 cut(s) 54, 476
TaqI TCGA 4 cut(s) 30, 423, 523, 739
TasI AATT 2 cut(s) 426, 936
TatI WGTACW 2 cut(s) 703, 1194
TauI GCSGC 9 cut(s) 113, 161, 209, 212, 215, 257, 281, 365, 719
TfiI GAWTC 5 cut(s) 367, 449, 621, 833, 1186
Tru1I TTAA 2 cut(s) 321, 456
Tru9I TTAA 2 cut(s) 321, 456
TscAI CASTG 2 cut(s) 846, 1180
TseFI GTSAC 1 cut(s) 175
TseI GCWGC 2 cut(s) 337, 980
Tsp45I GTSAC 1 cut(s) 175
TspDTI ATGAA 3 cut(s) 13, 665, 974
TspGWI ACGGA 1 cut(s) 235
TspMI CCCGGG 1 cut(s) 153
TspRI CASTG 2 cut(s) 846, 1180
Van91I CCANNNNNTGG 1 cut(s) 186
Vha464I CTTAAG 1 cut(s) 455
VpaK11BI GGWCC 2 cut(s) 393, 1018
XbaI TCTAGA 3 cut(s) 535, 686, 697
XcmI CCANNNNNNNNNTGG 1 cut(s) 412
XmaI CCCGGG 1 cut(s) 153
XmnI GAANNNNTTC 1 cut(s) 299
XspI CTAG 3 cut(s) 536, 687, 698
ZraI GACGTC 2 cut(s) 52, 474
ZrmI AGTACT 1 cut(s) 705
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.