AT1G73250

GDP-L-fucose synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0010988
Physical Location & Seq
Reverse (-)
27544969 .. 27546479
1511 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G73250.1

Sequence Viewer

Length: 972 bp
ATGGCGGAAACGATCGGCTCAGAGGTTTCGTCCATGTCTGACAAATCTGCCAAAATCTTCGTCGCGGGTCATCGTGGTTTGGTTGGATCTGCCATTGTCCGCAAGCTTCAGGAACAAGGTTTCACCAATCTCGTTCTTAAAACACACGCCGAGCTTGATCTCACTCGTCAAGCCGATGTTGAATCCTTCTTTTCTCAAGAGAAGCCAGTTTATGTAATCCTAGCAGCAGCTAAAGTTGGTGGTATTCACGCTAACAACACCTATCCTGCTGATTTCATTGGTGTCAATCTCCAGATTCAGACCAATGTGATCCACTCTGCATATGAGCACGGTGTGAAGAAGCTTCTCTTCCTTGGATCATCCTGCATTTACCCTAAATTTGCTCCTCAGCCAATTCCTGAGTCTGCTTTGTTAACAGCATCGCTTGAACCAACTAATGAGTGGTATGCTATTGCTAAGATCGCTGGGATTAAGACTTGTCAGGCTTATAGGATTCAGCACGGATGGGATGCAATCTCTGGCATGCCTACTAATCTCTATGGTCCTAATGACAATTTCCACCCGGAGAATTCTCATGTGCTTCCTGCTCTTATGAGGAGGTTCCACGAGGCGAAAGTGAATGGAGCGGAGGAAGTTGTGGTGTGGGGTACAGGTAGTCCGTTGAGGGAGTTCTTGCATGTTGATGATTTGGCTGATGCTTGTGTTTTCTTGCTGGATCGATACAGCGGGTTGGAGCATGTTAACATTGGAAGTGGTCAAGAAGTGACTATTAGAGAGTTGGCTGAGTTGGTGAAAGAGGTTGTTGGTTTTGAAGGGAAGCTTGGATGGGATTGCACTAAGCCAGATGGCACACCGAGGAAACTTATGGACAGCTCAAAGCTCGCGTCTTTGGGTTGGACACCTAAGGTTTCTCTTAGAGATGGTCTGAGCCAAACTTATGATTGGTATTTGAAGAATGTTTGCAACCGATAA

Protein Analysis

323

Amino Acids

35.57

Weight (kDa)

6.13

Isoelectric Point (pI)

26.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RmlD_sub_bind PF04321 18 - 133 5.6e-08 RmlD substrate binding domain
Epimerase PF01370 19 - 250 1.3e-64 NAD dependent epimerase/dehydratase family
GDP_Man_Dehyd PF16363 51 - 311 6.9e-17 GDP-mannose 4,6 dehydratase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010988)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G17890 AT1G17890 AT1G17890 AT1G73250
fragaria_vesca FvH4_4g08850 FvH4_4g08850 FvH4_4g08850
malus_domestica MD05G1099800.v1.1 MD10G1105500.v1.1
prunus_persica Prupe.1G100900_v2.0.a1
pyrus_communis pycom05g09860 pycom10g09090
rosa_chinensis RchiOBHm_Chr4g0405311
rosa_laevigata RLG00000008852
rosa_multiflora Rmu_sc0000161.1_g000028
rosa_roxburghii Rroxscaffold_5G00349690
rosa_rugosa Rorug04G0057300
rosa_samantha Rh4BG125000 Rh4CG137600
rosa_wichuraiana Rw4G010590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 626
AccII CGCG 2 cut(s) 65, 884
AciI CCGC 5 cut(s) 5, 65, 100, 626, 726
AclWI GGATC 4 cut(s) 94, 304, 364, 723
AcsI RAATTY 2 cut(s) 377, 568
AcuI CTGAAG 1 cut(s) 92
AdeI CACNNNGTG 1 cut(s) 334
AfaI GTAC 1 cut(s) 649
AgsI TTSAA 4 cut(s) 182, 428, 812, 952
AjuI GAANNNNNNNTTGG 2 cut(s) 804, 836
AluBI AGCT 7 cut(s) 106, 154, 230, 343, 820, 873, 880
AluI AGCT 7 cut(s) 106, 154, 230, 343, 820, 873, 880
Alw21I GWGCWC 1 cut(s) 330
AlwI GGATC 4 cut(s) 94, 304, 364, 723
ApeKI GCWGC 2 cut(s) 224, 227
ApoI RAATTY 2 cut(s) 377, 568
ArsI GACNNNNNNTTYG 4 cut(s) 45, 77, 869, 901
AspS9I GGNCC 1 cut(s) 542
AsuC2I CCSGG 1 cut(s) 563
AsuHPI GGTGA 2 cut(s) 115, 802
AvaII GGWCC 1 cut(s) 542
AxyI CCTNAGG 1 cut(s) 903
BauI CACGAG 1 cut(s) 605
Bbv12I GWGCWC 1 cut(s) 330
BbvCI CCTCAGC 1 cut(s) 387
BbvI GCAGC 2 cut(s) 236, 239
BccI CCATC 4 cut(s) 498, 819, 839, 914
BcnI CCSGG 1 cut(s) 563
BfaI CTAG 1 cut(s) 221
BisI GCNGC 2 cut(s) 225, 228
BlsI GCNGC 2 cut(s) 226, 229
Bme1390I CCNGG 1 cut(s) 563
Bme18I GGWCC 1 cut(s) 542
BmgT120I GGNCC 1 cut(s) 542
BmiI GGNNCC 1 cut(s) 602
BmrFI CCNGG 1 cut(s) 563
BmsI GCATC 3 cut(s) 428, 499, 685
BpmI CTGGAG 1 cut(s) 275
Bpu10I CCTNAGC 1 cut(s) 387
BpuEI CTTGAG 1 cut(s) 180
BpuMI CCSGG 1 cut(s) 563
Bsa29I ATCGAT 1 cut(s) 718
BsaJI CCNNGG 2 cut(s) 352, 854
Bse1I ACTGG 1 cut(s) 206
Bse21I CCTNAGG 1 cut(s) 903
BseCI ATCGAT 1 cut(s) 718
BseDI CCNNGG 2 cut(s) 352, 854
BseGI GGATG 4 cut(s) 359, 509, 514, 830
BseMII CTCAG 5 cut(s) 33, 390, 401, 774, 917
BseNI ACTGG 1 cut(s) 206
BseRI GAGGAG 2 cut(s) 375, 610
BseXI GCAGC 2 cut(s) 236, 239
BseYI CCCAGC 1 cut(s) 464
Bsh1236I CGCG 2 cut(s) 65, 884
Bsh1285I CGRYCG 1 cut(s) 15
BshVI ATCGAT 1 cut(s) 718
BsiEI CGRYCG 1 cut(s) 15
BsiHKAI GWGCWC 1 cut(s) 330
BsiSI CCGG 1 cut(s) 563
Bsp1286I GDGCHC 1 cut(s) 330
Bsp143I GATC 7 cut(s) 12, 86, 157, 309, 356, 459, 715
BspACI CCGC 5 cut(s) 5, 65, 100, 626, 726
BspCNI CTCAG 5 cut(s) 32, 391, 400, 775, 918
BspDI ATCGAT 1 cut(s) 718
BspFNI CGCG 2 cut(s) 65, 884
BspLI GGNNCC 1 cut(s) 602
BspPI GGATC 4 cut(s) 94, 304, 364, 723
BsrBI CCGCTC 1 cut(s) 626
BsrI ACTGG 1 cut(s) 206
BssECI CCNNGG 2 cut(s) 352, 854
BssMI GATC 7 cut(s) 12, 86, 157, 309, 356, 459, 715
BssSI CACGAG 1 cut(s) 605
BssT1I CCWWGG 1 cut(s) 352
Bst2BI CACGAG 1 cut(s) 605
Bst4CI ACNGT 1 cut(s) 332
Bst6I CTCTTC 1 cut(s) 353
BstC8I GCNNGC 3 cut(s) 104, 524, 882
BstDEI CTNAG 9 cut(s) 19, 387, 399, 456, 783, 837, 903, 914, 926
BstF5I GGATG 4 cut(s) 359, 509, 514, 830
BstFNI CGCG 2 cut(s) 65, 884
BstKTI GATC 7 cut(s) 15, 89, 160, 312, 359, 462, 718
BstMBI GATC 7 cut(s) 12, 86, 157, 309, 356, 459, 715
BstMCI CGRYCG 1 cut(s) 15
BstMWI GCNNNNNNNGC 1 cut(s) 461
BstNSI RCATGY 3 cut(s) 526, 680, 740
BstSCI CCNGG 1 cut(s) 561
BstUI CGCG 2 cut(s) 65, 884
BstV1I GCAGC 2 cut(s) 236, 239
BstX2I RGATCY 1 cut(s) 86
BstYI RGATCY 1 cut(s) 86
Bsu15I ATCGAT 1 cut(s) 718
Bsu36I CCTNAGG 1 cut(s) 903
BsuTUI ATCGAT 1 cut(s) 718
BtgZI GCGATG 1 cut(s) 405
BtsCI GGATG 4 cut(s) 359, 509, 514, 830
Cac8I GCNNGC 3 cut(s) 104, 524, 882
Cfr13I GGNCC 1 cut(s) 542
ClaI ATCGAT 1 cut(s) 718
CseI GACGC 1 cut(s) 873
Csp6I GTAC 1 cut(s) 648
CviAII CATG 5 cut(s) 34, 523, 575, 677, 737
CviQI GTAC 1 cut(s) 648
DdeI CTNAG 9 cut(s) 19, 387, 399, 456, 783, 837, 903, 914, 926
DpnI GATC 7 cut(s) 14, 88, 159, 311, 358, 461, 717
DpnII GATC 7 cut(s) 12, 86, 157, 309, 356, 459, 715
DraIII CACNNNGTG 1 cut(s) 334
Eam1104I CTCTTC 1 cut(s) 353
EarI CTCTTC 1 cut(s) 353
EciI GGCGGA 1 cut(s) 20
Eco130I CCWWGG 1 cut(s) 352
Eco47I GGWCC 1 cut(s) 542
Eco57I CTGAAG 1 cut(s) 92
Eco81I CCTNAGG 1 cut(s) 903
EcoRI GAATTC 1 cut(s) 568
EcoT14I CCWWGG 1 cut(s) 352
ErhI CCWWGG 1 cut(s) 352
FaeI CATG 5 cut(s) 37, 526, 578, 680, 740
FalI AAGNNNNNCTT 4 cut(s) 332, 364, 804, 836
FatI CATG 5 cut(s) 33, 522, 574, 676, 736
FauI CCCGC 2 cut(s) 58, 719
FauNDI CATATG 1 cut(s) 322
Fnu4HI GCNGC 2 cut(s) 225, 228
FokI GGATG 4 cut(s) 346, 516, 521, 837
Fsp4HI GCNGC 2 cut(s) 225, 228
FspBI CTAG 1 cut(s) 221
GluI GCNGC 2 cut(s) 225, 228
GsaI CCCAGC 1 cut(s) 468
GsuI CTGGAG 1 cut(s) 275
HapII CCGG 1 cut(s) 563
HgaI GACGC 1 cut(s) 873
Hin1II CATG 5 cut(s) 37, 526, 578, 680, 740
HincII GTYRAC 2 cut(s) 414, 742
HindII GTYRAC 2 cut(s) 414, 742
HindIII AAGCTT 3 cut(s) 104, 341, 818
HinfI GANTC 4 cut(s) 182, 295, 401, 493
HpaI GTTAAC 2 cut(s) 414, 742
HpaII CCGG 1 cut(s) 563
HphI GGTGA 2 cut(s) 115, 802
Hpy166II GTNNAC 2 cut(s) 414, 742
Hpy188I TCNGA 4 cut(s) 22, 40, 300, 927
Hpy188III TCNNGA 5 cut(s) 110, 197, 292, 398, 758
Hpy8I GTNNAC 2 cut(s) 414, 742
Hpy99I CGWCG 1 cut(s) 65
HpyAV CCTTC 2 cut(s) 196, 806
HpyCH4III ACNGT 1 cut(s) 332
HpyCH4V TGCA 6 cut(s) 320, 366, 512, 676, 834, 963
HpyF10VI GCNNNNNNNGC 1 cut(s) 461
HpyF3I CTNAG 9 cut(s) 19, 387, 399, 456, 783, 837, 903, 914, 926
Hsp92II CATG 5 cut(s) 37, 526, 578, 680, 740
KspAI GTTAAC 2 cut(s) 414, 742
Kzo9I GATC 7 cut(s) 12, 86, 157, 309, 356, 459, 715
LmnI GCTCC 3 cut(s) 388, 623, 733
Lsp1109I GCAGC 2 cut(s) 236, 239
LweI GCATC 3 cut(s) 428, 499, 685
MaeI CTAG 1 cut(s) 221
MaeIII GTNAC 1 cut(s) 763
MalI GATC 7 cut(s) 14, 88, 159, 311, 358, 461, 717
MbiI CCGCTC 1 cut(s) 626
MboI GATC 7 cut(s) 12, 86, 157, 309, 356, 459, 715
MboII GAAGA 4 cut(s) 49, 340, 349, 964
MflI RGATCY 1 cut(s) 86
MhlI GDGCHC 1 cut(s) 330
MluCI AATT 4 cut(s) 377, 393, 553, 568
MlyI GAGTC 1 cut(s) 410
MmeI TCCRAC 3 cut(s) 64, 711, 875
MnlI CCTC 9 cut(s) 16, 396, 588, 591, 601, 622, 657, 790, 849
MseI TTAA 4 cut(s) 138, 413, 471, 741
MslI CAYNNNNRTG 1 cut(s) 681
MspA1I CMGCKG 1 cut(s) 726
MspI CCGG 1 cut(s) 563
MspR9I CCNGG 1 cut(s) 563
MvnI CGCG 2 cut(s) 65, 884
MwoI GCNNNNNNNGC 1 cut(s) 461
NciI CCSGG 1 cut(s) 563
NdeI CATATG 1 cut(s) 322
NdeII GATC 7 cut(s) 12, 86, 157, 309, 356, 459, 715
NlaIII CATG 5 cut(s) 37, 526, 578, 680, 740
NlaIV GGNNCC 1 cut(s) 602
NmeAIII GCCGAG 1 cut(s) 175
NmuCI GTSAC 1 cut(s) 763
NspI RCATGY 3 cut(s) 526, 680, 740
PaeI GCATGC 1 cut(s) 526
PfeI GAWTC 3 cut(s) 182, 295, 493
PkrI GCNGC 2 cut(s) 226, 229
Ple19I CGATCG 1 cut(s) 15
PleI GAGTC 1 cut(s) 409
PpsI GAGTC 1 cut(s) 409
PspFI CCCAGC 1 cut(s) 464
PspN4I GGNNCC 1 cut(s) 602
PspPI GGNCC 1 cut(s) 542
PsuI RGATCY 1 cut(s) 86
PvuI CGATCG 1 cut(s) 15
RsaI GTAC 1 cut(s) 649
RsaNI GTAC 1 cut(s) 648
RseI CAYNNNNRTG 1 cut(s) 681
SaqAI TTAA 4 cut(s) 138, 413, 471, 741
SatI GCNGC 2 cut(s) 225, 228
Sau3AI GATC 7 cut(s) 12, 86, 157, 309, 356, 459, 715
Sau96I GGNCC 1 cut(s) 542
SchI GAGTC 1 cut(s) 410
ScrFI CCNGG 1 cut(s) 563
SduI GDGCHC 1 cut(s) 330
SfaNI GCATC 3 cut(s) 428, 499, 685
SinI GGWCC 1 cut(s) 542
SmiMI CAYNNNNRTG 1 cut(s) 681
SmlI CTYRAG 1 cut(s) 195
SmoI CTYRAG 1 cut(s) 195
SphI GCATGC 1 cut(s) 526
Sse9I AATT 4 cut(s) 377, 393, 553, 568
SsiI CCGC 5 cut(s) 5, 65, 100, 626, 726
SspMI CTAG 1 cut(s) 221
StyD4I CCNGG 1 cut(s) 561
StyI CCWWGG 1 cut(s) 352
TaaI ACNGT 1 cut(s) 332
TaqI TCGA 1 cut(s) 718
TasI AATT 4 cut(s) 377, 393, 553, 568
TfiI GAWTC 3 cut(s) 182, 295, 493
Tru1I TTAA 4 cut(s) 138, 413, 471, 741
Tru9I TTAA 4 cut(s) 138, 413, 471, 741
TseFI GTSAC 1 cut(s) 763
TseI GCWGC 2 cut(s) 224, 227
Tsp45I GTSAC 1 cut(s) 763
TspDTI ATGAA 1 cut(s) 265
TspGWI ACGGA 2 cut(s) 516, 648
VpaK11BI GGWCC 1 cut(s) 542
XapI RAATTY 2 cut(s) 377, 568
XceI RCATGY 3 cut(s) 526, 680, 740
XcmI CCANNNNNNNNNTGG 1 cut(s) 438
XspI CTAG 1 cut(s) 221
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.