pycom05g09860

GDP-L-fucose synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0010988
Physical Location & Seq
Reverse (-)
13138246 .. 13139319
1074 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g09860.2

Sequence Viewer

Length: 924 bp
ATGAGTGGCGCCAATAACGATGCCACTTCGCCTTCCTTCCTCTCCGACAAATCGGCCAGGATCTTCGTAGCCGGCCACCGCGGCCTCGTCGGCTCCGCCATCATCCGCAAGCTCCAAGCCCTAGGCTTCACCAACCTTGTCCTCCGCACCCACGCCGAGCTCGACCTCACCCGCCAAGCCGACGTCGAGTCCTTCTTCGCCGCCGAGAAACCCCGATTCGTGATCCTCGCCGCCGCTAAAGTCGGCGGCATTCACGCCAACAACACCTACCCGGCGGATTTCATCGCCGTCAATCTCCAGATCCAGACCAACGTCATCGACGCCGCCTACCGATTCGGAGTCAAGAAGCTCTTGTTCCTCGGCTCCTCCTGCATTTACCCCAAATTCGCTCCTCAGCCCATCCCGGAGAACGCATTGCTGACGGGCCCGCTCGAGCCCACGAACGAGTGGTACGCCATTGCCAAGATCGCCGGGATCAAGATGTGCCAGGCGTATCGGATTCAGTACAATTGGGATGCAATTTCTGGGATGCCCACCAATTTGTACGGGCCGCATGATAACTTTCACCCCGAAAATTCGCACGTTTTGCCGGCACTGATGAGGCGGTTTCATGAGGCAAAGGTGAAGGGTGCAGAGGAGGTGGTGGTGTGGGGGACCGGAAGCCCCTTGAGGGAGTTTTTGCATGTCGATGATTTGGCGGATGGGGTGGTGTTCATGATGGAGAGTTACAGTGGGTTGGAGCATGTCAATGTGGGGAGTGGCAAGGAAGTGACTATCAAGGAGTTGGCTGAACTTGTCAAGGAGGTTGTTGGGTTCGAGGGACGGCTTGTCTGGGACTCCACTAAGCCGGATGGAACTCCGAGGAAGCTCATGGATAGCTCAAAGCTCGCAGGCTTGGGATGGACACCAAAGATCGATCGCTGA

Protein Analysis

308

Amino Acids

33.71

Weight (kDa)

6.76

Isoelectric Point (pI)

20.27

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010988)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G17890 AT1G17890 AT1G17890 AT1G73250
fragaria_vesca FvH4_4g08850 FvH4_4g08850 FvH4_4g08850
malus_domestica MD05G1099800.v1.1 MD10G1105500.v1.1
prunus_persica Prupe.1G100900_v2.0.a1
pyrus_communis pycom05g09860 pycom10g09090
rosa_chinensis RchiOBHm_Chr4g0405311
rosa_laevigata RLG00000008852
rosa_multiflora Rmu_sc0000161.1_g000028
rosa_roxburghii Rroxscaffold_5G00349690
rosa_rugosa Rorug04G0057300
rosa_samantha Rh4BG125000 Rh4CG137600
rosa_wichuraiana Rw4G010590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 186
AccB1I GGYRCC 1 cut(s) 8
AccBSI CCGCTC 1 cut(s) 430
AccII CGCG 1 cut(s) 81
AclWI GGATC 4 cut(s) 68, 217, 295, 482
AcoI YGGCCR 2 cut(s) 54, 73
AcsI RAATTY 2 cut(s) 383, 574
AcyI GRCGYC 3 cut(s) 9, 183, 321
AfaI GTAC 3 cut(s) 452, 506, 545
AfiI CCNNNNNNNGG 1 cut(s) 670
AhdI GACNNNNNGTC 2 cut(s) 187, 827
AjnI CCWGG 2 cut(s) 56, 486
AluBI AGCT 6 cut(s) 112, 160, 349, 868, 879, 886
AluI AGCT 6 cut(s) 112, 160, 349, 868, 879, 886
Alw21I GWGCWC 1 cut(s) 162
AlwI GGATC 4 cut(s) 68, 217, 295, 482
Ama87I CYCGRG 1 cut(s) 431
AoxI GGCC 5 cut(s) 54, 73, 82, 424, 548
ApaI GGGCCC 1 cut(s) 428
ApoI RAATTY 2 cut(s) 383, 574
AspA2I CCTAGG 1 cut(s) 121
AspLEI GCGC 1 cut(s) 11
AspS9I GGNCC 4 cut(s) 424, 425, 548, 654
AsuC2I CCSGG 3 cut(s) 272, 404, 472
AsuHPI GGTGA 4 cut(s) 121, 160, 557, 634
AvaI CYCGRG 1 cut(s) 431
AvaII GGWCC 1 cut(s) 654
AvrII CCTAGG 1 cut(s) 121
BaeGI GKGCMC 1 cut(s) 428
BanI GGYRCC 1 cut(s) 8
BanII GRGCYC 3 cut(s) 162, 428, 438
Bbv12I GWGCWC 1 cut(s) 162
BbvCI CCTCAGC 1 cut(s) 393
BccI CCATC 6 cut(s) 107, 407, 695, 712, 845, 894
BceAI ACGGC 2 cut(s) 272, 839
BciT130I CCWGG 2 cut(s) 58, 488
BcnI CCSGG 3 cut(s) 272, 404, 472
BfaI CTAG 1 cut(s) 122
BfoI RGCGCY 1 cut(s) 12
BglI GCCNNNNNGGC 2 cut(s) 81, 90
BisI GCNGC 7 cut(s) 82, 201, 231, 234, 247, 324, 551
BlnI CCTAGG 1 cut(s) 121
BlsI GCNGC 7 cut(s) 83, 202, 232, 235, 248, 325, 552
Bme1390I CCNGG 5 cut(s) 58, 272, 404, 472, 488
Bme18I GGWCC 1 cut(s) 654
BmeRI GACNNNNNGTC 2 cut(s) 187, 827
BmeT110I CYCGRG 1 cut(s) 431
BmgT120I GGNCC 4 cut(s) 424, 425, 548, 654
BmiI GGNNCC 5 cut(s) 10, 94, 364, 426, 655
BmrFI CCNGG 5 cut(s) 58, 272, 404, 472, 488
BmsI GCATC 3 cut(s) 10, 505, 519
BoxI GACNNNNGTC 1 cut(s) 311
BpmI CTGGAG 1 cut(s) 281
Bpu10I CCTNAGC 1 cut(s) 393
BpuEI CTTGAG 1 cut(s) 688
BpuMI CCSGG 3 cut(s) 272, 404, 472
Bsa29I ATCGAT 1 cut(s) 915
BsaHI GRCGYC 3 cut(s) 9, 183, 321
BsaJI CCNNGG 4 cut(s) 79, 121, 358, 860
BsaWI WCCGGW 1 cut(s) 656
Bsc4I CCNNNNNNNGG 1 cut(s) 670
Bse118I RCCGGY 2 cut(s) 71, 589
Bse3DI GCAATG 2 cut(s) 413, 456
BseBI CCWGG 2 cut(s) 58, 488
BseCI ATCGAT 1 cut(s) 915
BseDI CCNNGG 4 cut(s) 79, 121, 358, 860
BseGI GGATG 7 cut(s) 102, 399, 520, 534, 706, 856, 905
BseLI CCNNNNNNNGG 1 cut(s) 670
BseMI GCAATG 2 cut(s) 413, 456
BseMII CTCAG 1 cut(s) 407
BseRI GAGGAG 3 cut(s) 355, 381, 650
BseSI GKGCMC 1 cut(s) 428
BsgI GTGCAG 1 cut(s) 651
Bsh1236I CGCG 1 cut(s) 81
Bsh1285I CGRYCG 1 cut(s) 919
BshFI GGCC 5 cut(s) 56, 75, 84, 426, 550
BshNI GGYRCC 1 cut(s) 8
BshVI ATCGAT 1 cut(s) 915
BsiEI CGRYCG 1 cut(s) 919
BsiHKAI GWGCWC 1 cut(s) 162
BsiHKCI CYCGRG 1 cut(s) 431
BsiSI CCGG 7 cut(s) 72, 272, 404, 471, 590, 657, 848
BslFI GGGAC 3 cut(s) 667, 834, 848
BslI CCNNNNNNNGG 1 cut(s) 670
BsmFI GGGAC 3 cut(s) 667, 834, 848
BsmI GAATGC 1 cut(s) 249
BsnI GGCC 5 cut(s) 56, 75, 84, 426, 550
BsoBI CYCGRG 1 cut(s) 431
Bsp120I GGGCCC 1 cut(s) 424
Bsp1286I GDGCHC 3 cut(s) 162, 428, 438
Bsp143I GATC 7 cut(s) 60, 222, 300, 465, 474, 912, 916
BspANI GGCC 5 cut(s) 56, 75, 84, 426, 550
BspCNI CTCAG 1 cut(s) 406
BspDI ATCGAT 1 cut(s) 915
BspFNI CGCG 1 cut(s) 81
BspHI TCATGA 2 cut(s) 610, 714
BspLI GGNNCC 5 cut(s) 10, 94, 364, 426, 655
BspPI GGATC 4 cut(s) 68, 217, 295, 482
BspT107I GGYRCC 1 cut(s) 8
BsrBI CCGCTC 1 cut(s) 430
BsrDI GCAATG 2 cut(s) 413, 456
BsrFI RCCGGY 2 cut(s) 71, 589
BssAI RCCGGY 2 cut(s) 71, 589
BssECI CCNNGG 4 cut(s) 79, 121, 358, 860
BssMI GATC 7 cut(s) 60, 222, 300, 465, 474, 912, 916
BssNI GRCGYC 3 cut(s) 9, 183, 321
BssT1I CCWWGG 1 cut(s) 121
Bst2UI CCWGG 2 cut(s) 58, 488
Bst4CI ACNGT 1 cut(s) 731
BstACI GRCGYC 3 cut(s) 9, 183, 321
BstAPI GCANNNNNTGC 1 cut(s) 586
BstC8I GCNNGC 6 cut(s) 73, 110, 428, 591, 888, 892
BstDEI CTNAG 2 cut(s) 393, 843
BstDSI CCRYGG 1 cut(s) 79
BstF5I GGATG 7 cut(s) 102, 399, 520, 534, 706, 856, 905
BstFNI CGCG 1 cut(s) 81
BstH2I RGCGCY 1 cut(s) 12
BstHHI GCGC 1 cut(s) 11
BstKTI GATC 7 cut(s) 63, 225, 303, 468, 477, 915, 919
BstMBI GATC 7 cut(s) 60, 222, 300, 465, 474, 912, 916
BstMCI CGRYCG 1 cut(s) 919
BstMWI GCNNNNNNNGC 5 cut(s) 81, 90, 369, 467, 586
BstNI CCWGG 2 cut(s) 58, 488
BstNSI RCATGY 2 cut(s) 686, 746
BstPAI GACNNNNGTC 1 cut(s) 311
BstSCI CCNGG 5 cut(s) 56, 270, 402, 470, 486
BstSLI GKGCMC 1 cut(s) 428
BstUI CGCG 1 cut(s) 81
BstX2I RGATCY 2 cut(s) 60, 300
BstYI RGATCY 2 cut(s) 60, 300
Bsu15I ATCGAT 1 cut(s) 915
BsuRI GGCC 5 cut(s) 56, 75, 84, 426, 550
BsuTUI ATCGAT 1 cut(s) 915
BtgI CCRYGG 1 cut(s) 79
BtgZI GCGATG 1 cut(s) 268
BtsCI GGATG 7 cut(s) 102, 399, 520, 534, 706, 856, 905
BtsIMutI CAGTG 2 cut(s) 593, 736
Cac8I GCNNGC 6 cut(s) 73, 110, 428, 591, 888, 892
CciI TCATGA 2 cut(s) 610, 714
CfoI GCGC 1 cut(s) 11
Cfr10I RCCGGY 2 cut(s) 71, 589
Cfr13I GGNCC 4 cut(s) 424, 425, 548, 654
Cfr42I CCGCGG 1 cut(s) 82
ClaI ATCGAT 1 cut(s) 915
CseI GACGC 1 cut(s) 329
Csp6I GTAC 3 cut(s) 451, 505, 544
CviAII CATG 6 cut(s) 554, 611, 683, 715, 743, 871
CviQI GTAC 3 cut(s) 451, 505, 544
DdeI CTNAG 2 cut(s) 393, 843
DinI GGCGCC 1 cut(s) 10
DpnI GATC 7 cut(s) 62, 224, 302, 467, 476, 914, 918
DpnII GATC 7 cut(s) 60, 222, 300, 465, 474, 912, 916
DriI GACNNNNNGTC 2 cut(s) 187, 827
EaeI YGGCCR 2 cut(s) 54, 73
Eam1105I GACNNNNNGTC 2 cut(s) 187, 827
EciI GGCGGA 3 cut(s) 85, 290, 713
Ecl136II GAGCTC 1 cut(s) 160
Eco130I CCWWGG 1 cut(s) 121
Eco24I GRGCYC 3 cut(s) 162, 428, 438
Eco47I GGWCC 1 cut(s) 654
Eco53kI GAGCTC 1 cut(s) 160
Eco88I CYCGRG 1 cut(s) 431
EcoICRI GAGCTC 1 cut(s) 160
EcoRII CCWGG 2 cut(s) 56, 486
EcoT14I CCWWGG 1 cut(s) 121
EcoT38I GRGCYC 3 cut(s) 162, 428, 438
EgeI GGCGCC 1 cut(s) 10
EheI GGCGCC 1 cut(s) 10
ErhI CCWWGG 1 cut(s) 121
FaeI CATG 6 cut(s) 557, 614, 686, 718, 746, 874
FaiI YATR 6 cut(s) 555, 612, 684, 716, 744, 872
FalI AAGNNNNNCTT 2 cut(s) 335, 367
FaqI GGGAC 3 cut(s) 667, 834, 848
FatI CATG 6 cut(s) 553, 610, 682, 714, 742, 870
FauI CCCGC 2 cut(s) 179, 435
Fnu4HI GCNGC 7 cut(s) 82, 201, 231, 234, 247, 324, 551
FokI GGATG 7 cut(s) 89, 386, 527, 541, 713, 863, 912
FriOI GRGCYC 3 cut(s) 162, 428, 438
Fsp4HI GCNGC 7 cut(s) 82, 201, 231, 234, 247, 324, 551
FspBI CTAG 1 cut(s) 122
GlaI GCGC 1 cut(s) 10
GluI GCNGC 7 cut(s) 82, 201, 231, 234, 247, 324, 551
GsuI CTGGAG 1 cut(s) 281
HaeII RGCGCY 1 cut(s) 12
HaeIII GGCC 5 cut(s) 56, 75, 84, 426, 550
HapII CCGG 7 cut(s) 72, 272, 404, 471, 590, 657, 848
HgaI GACGC 1 cut(s) 329
HhaI GCGC 1 cut(s) 11
Hin1I GRCGYC 3 cut(s) 9, 183, 321
Hin1II CATG 6 cut(s) 557, 614, 686, 718, 746, 874
Hin6I GCGC 1 cut(s) 9
HinP1I GCGC 1 cut(s) 9
HinfI GANTC 6 cut(s) 188, 216, 333, 339, 499, 836
HpaII CCGG 7 cut(s) 72, 272, 404, 471, 590, 657, 848
HphI GGTGA 4 cut(s) 121, 160, 557, 634
Hpy188I TCNGA 4 cut(s) 46, 338, 498, 861
Hpy188III TCNNGA 7 cut(s) 220, 298, 304, 343, 478, 611, 715
Hpy99I CGWCG 4 cut(s) 92, 185, 188, 323
HpyAV CCTTC 4 cut(s) 42, 46, 202, 619
HpyCH4III ACNGT 1 cut(s) 731
HpyCH4IV ACGT 3 cut(s) 183, 312, 582
HpyCH4V TGCA 4 cut(s) 372, 518, 632, 682
HpyF10VI GCNNNNNNNGC 5 cut(s) 81, 90, 369, 467, 586
HpyF3I CTNAG 2 cut(s) 393, 843
HpySE526I ACGT 3 cut(s) 183, 312, 582
Hsp92I GRCGYC 3 cut(s) 9, 183, 321
Hsp92II CATG 6 cut(s) 557, 614, 686, 718, 746, 874
HspAI GCGC 1 cut(s) 9
KasI GGCGCC 1 cut(s) 8
KroI GCCGGC 2 cut(s) 71, 589
KroNI GCCGGC 2 cut(s) 73, 591
KspI CCGCGG 1 cut(s) 82
Kzo9I GATC 7 cut(s) 60, 222, 300, 465, 474, 912, 916
LmnI GCTCC 5 cut(s) 98, 117, 368, 394, 739
LweI GCATC 3 cut(s) 10, 505, 519
MaeI CTAG 1 cut(s) 122
MaeII ACGT 3 cut(s) 183, 312, 582
MaeIII GTNAC 2 cut(s) 725, 769
MalI GATC 7 cut(s) 62, 224, 302, 467, 476, 914, 918
MbiI CCGCTC 1 cut(s) 430
MboI GATC 7 cut(s) 60, 222, 300, 465, 474, 912, 916
MboII GAAGA 2 cut(s) 55, 187
MfeI CAATTG 1 cut(s) 508
MflI RGATCY 2 cut(s) 60, 300
MhlI GDGCHC 3 cut(s) 162, 428, 438
MluCI AATT 5 cut(s) 383, 508, 519, 538, 574
Mly113I GGCGCC 1 cut(s) 9
MlyI GAGTC 3 cut(s) 197, 348, 830
MmeI TCCRAC 2 cut(s) 69, 717
MroNI GCCGGC 2 cut(s) 71, 589
MslI CAYNNNNRTG 2 cut(s) 687, 747
MspA1I CMGCKG 1 cut(s) 81
MspI CCGG 7 cut(s) 72, 272, 404, 471, 590, 657, 848
MspR9I CCNGG 5 cut(s) 58, 272, 404, 472, 488
MunI CAATTG 1 cut(s) 508
Mva1269I GAATGC 1 cut(s) 249
MvaI CCWGG 2 cut(s) 58, 488
MvnI CGCG 1 cut(s) 81
MwoI GCNNNNNNNGC 5 cut(s) 81, 90, 369, 467, 586
NaeI GCCGGC 2 cut(s) 73, 591
NarI GGCGCC 1 cut(s) 9
NciI CCSGG 3 cut(s) 272, 404, 472
NdeII GATC 7 cut(s) 60, 222, 300, 465, 474, 912, 916
NgoMIV GCCGGC 2 cut(s) 71, 589
NlaIII CATG 6 cut(s) 557, 614, 686, 718, 746, 874
NlaIV GGNNCC 5 cut(s) 10, 94, 364, 426, 655
NmeAIII GCCGAG 3 cut(s) 181, 229, 339
NmuCI GTSAC 1 cut(s) 769
NspI RCATGY 2 cut(s) 686, 746
PaeR7I CTCGAG 1 cut(s) 431
PagI TCATGA 2 cut(s) 610, 714
PcsI WCGNNNNNNNCGW 1 cut(s) 159
PctI GAATGC 1 cut(s) 249
PdiI GCCGGC 2 cut(s) 73, 591
PfeI GAWTC 3 cut(s) 216, 333, 499
PfoI TCCNGGA 1 cut(s) 402
PkrI GCNGC 7 cut(s) 83, 202, 232, 235, 248, 325, 552
Ple19I CGATCG 1 cut(s) 919
PleI GAGTC 3 cut(s) 196, 347, 830
PluTI GGCGCC 1 cut(s) 12
PpsI GAGTC 3 cut(s) 196, 347, 830
PshAI GACNNNNGTC 1 cut(s) 311
Psp124BI GAGCTC 1 cut(s) 162
Psp6I CCWGG 2 cut(s) 56, 486
PspGI CCWGG 2 cut(s) 56, 486
PspN4I GGNNCC 5 cut(s) 10, 94, 364, 426, 655
PspOMI GGGCCC 1 cut(s) 424
PspPI GGNCC 4 cut(s) 424, 425, 548, 654
PspXI VCTCGAGB 1 cut(s) 431
PsrI GAACNNNNNNTAC 2 cut(s) 434, 466
PsuI RGATCY 2 cut(s) 60, 300
PvuI CGATCG 1 cut(s) 919
RsaI GTAC 3 cut(s) 452, 506, 545
RsaNI GTAC 3 cut(s) 451, 505, 544
RseI CAYNNNNRTG 2 cut(s) 687, 747
SacI GAGCTC 1 cut(s) 162
SacII CCGCGG 1 cut(s) 82
SatI GCNGC 7 cut(s) 82, 201, 231, 234, 247, 324, 551
Sau3AI GATC 7 cut(s) 60, 222, 300, 465, 474, 912, 916
Sau96I GGNCC 4 cut(s) 424, 425, 548, 654
SchI GAGTC 3 cut(s) 197, 348, 830
ScrFI CCNGG 5 cut(s) 58, 272, 404, 472, 488
SduI GDGCHC 3 cut(s) 162, 428, 438
SfaNI GCATC 3 cut(s) 10, 505, 519
SfiI GGCCNNNNNGGCC 1 cut(s) 81
SfoI GGCGCC 1 cut(s) 10
Sfr274I CTCGAG 1 cut(s) 431
Sfr303I CCGCGG 1 cut(s) 82
SgrBI CCGCGG 1 cut(s) 82
SinI GGWCC 1 cut(s) 654
SlaI CTCGAG 1 cut(s) 431
SmiMI CAYNNNNRTG 2 cut(s) 687, 747
SmlI CTYRAG 2 cut(s) 431, 667
SmoI CTYRAG 2 cut(s) 431, 667
Sse9I AATT 5 cut(s) 383, 508, 519, 538, 574
SspDI GGCGCC 1 cut(s) 8
SspMI CTAG 1 cut(s) 122
SstI GAGCTC 1 cut(s) 162
StyD4I CCNGG 5 cut(s) 56, 270, 402, 470, 486
StyI CCWWGG 1 cut(s) 121
TaaI ACNGT 1 cut(s) 731
TaiI ACGT 3 cut(s) 186, 315, 585
TaqI TCGA 7 cut(s) 162, 186, 318, 432, 687, 816, 915
TasI AATT 5 cut(s) 383, 508, 519, 538, 574
TatI WGTACW 1 cut(s) 504
TauI GCSGC 7 cut(s) 84, 203, 233, 236, 249, 326, 553
TfiI GAWTC 3 cut(s) 216, 333, 499
TscAI CASTG 2 cut(s) 600, 736
TseFI GTSAC 1 cut(s) 769
Tsp45I GTSAC 1 cut(s) 769
TspDTI ATGAA 3 cut(s) 271, 599, 703
TspRI CASTG 2 cut(s) 600, 736
VpaK11BI GGWCC 1 cut(s) 654
XapI RAATTY 2 cut(s) 383, 574
XceI RCATGY 2 cut(s) 686, 746
XhoI CTCGAG 1 cut(s) 431
XmaJI CCTAGG 1 cut(s) 121
XspI CTAG 1 cut(s) 122
ZraI GACGTC 1 cut(s) 184
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.