Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0010988

Physical Location & Seq

Genomic Coordinates Reverse (-)

17236235 .. 17237661

Gene Structure 1427 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1105500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 975 bp

ATGAGTGGCGCCAAGAACAATGCCACTTCGCCTTCCTTCCTCTCTGACAAATCGGCGAAGGTTTTCGTAGCCGGCCACCGCGGCCTCGTCGGCTCCGCCATCGTCCGCAAGCTCCATGCCCTAGGCTTCACCAGCCTCGTCCTCCGCACCCACGCCGAGCTCGACCTCACTCGCCAGGCCGACGTCGAGTCCTTTTTCGCCGCCGAGAAACCCCAATTCTTGATCCTCGCCGCCGCTAAAGTCGGTGGCATTCACGCCAACAACACGTACCCGGCGGATTTCATCGCCGTCAATCTCCAGATCCAGACCAATGTCATAGACGCCGCCTACCGATTTGGAGTCAAGAAGCTCTTGTTCCTTGGCTCCTCCTGCATTTACCCCAAATTCGCTCCTCAACCCATCCCGGAGAACGCATTGCTGACGGGACCACTGGAGCCTACCAACGAGTGGTACGCCATCGCCAAGATCGCCGGGATCAAGATGTGCCAGGCGTATCGGATTCAGTACAGTTGGGATGCAATTTCTGGGATGCCCACCAATTTGTATGGGCCGCATGACAACTTTCACCCTGAAAATTCGCACGTGTTGCCGGCATTGATGAGGCGGTTTCACGAGGCAAAGGTGAAAGGGGCGGAGGAGGTGGTGGTGTGGGGGACTGGAAGCCCCCTGAGAGAGTTTTTGCACGTGGACGATTTGGCAGATGGAGTGGTGTTCATGATGGACAGTTATAGTGGGTTGGAGCATGTCAATGTGGGGAGTGGCAAGGAAGTGACAATTAAGGAGTTGGCTGAGCTTGTCAAGGAGGTTGTTGGGTTCGAGGGCCGACTTGTTTGGGACTCTACCAAGCCCGATGGAACTCCGAGGAAGCTCATGGATAGCTCAAAGCTTGCAGGGTTGGGGTGGACACCGAAGATCTCGCTGAAAGACGGGCTTGTTGATACTTATAGCTGGTACTTGGAGAATGTCAAGCAATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005975 GO:0005996 GO:0006004 GO:0006005 GO:0006139 GO:0006725 GO:0006793 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009225 GO:0009226 GO:0009987 GO:0016051 GO:0016491 GO:0016614 GO:0016616 GO:0018130 GO:0019318 GO:0019319 GO:0019438 GO:0034641 GO:0034654 GO:0042350 GO:0042353 GO:0042354 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044283 GO:0046364 GO:0046368 GO:0046483 GO:0050577 GO:0055086 GO:0055114 GO:0071704 GO:1901135 GO:1901137 GO:1901360 GO:1901362 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00051 ko00520 ko01100 map00051 map00520 map01100

Pfam Domains

Protein Families

Epimerase

Protein Analysis

325

Amino Acids

35.56

Weight (kDa)

6.8

Isoelectric Point (pI)

19.26

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RmlD_sub_bind	PF04321	20 - 136	1e-06	RmlD substrate binding domain
Epimerase	PF01370	21 - 252	5.1e-64	NAD dependent epimerase/dehydratase family
GDP_Man_Dehyd	PF16363	53 - 311	4e-18	GDP-mannose 4,6 dehydratase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0010988)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G17890 AT1G17890 AT1G17890 AT1G73250
fragaria_vesca	FvH4_4g08850 FvH4_4g08850 FvH4_4g08850
malus_domestica	MD05G1099800.v1.1 MD10G1105500.v1.1
prunus_persica	Prupe.1G100900_v2.0.a1
pyrus_communis	pycom05g09860 pycom10g09090
rosa_chinensis	RchiOBHm_Chr4g0405311
rosa_laevigata	RLG00000008852
rosa_multiflora	Rmu_sc0000161.1_g000028
rosa_roxburghii	Rroxscaffold_5G00349690
rosa_rugosa	Rorug04G0057300
rosa_samantha	Rh4BG125000 Rh4CG137600
rosa_wichuraiana	Rw4G010590

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	186
AccB1I	GGYRCC	1 cut(s)	8
AccB7I	CCANNNNNTGG	1 cut(s)	447
AccII	CGCG	1 cut(s)	81
AclWI	GGATC	3 cut(s)	217, 295, 482
AcoI	YGGCCR	1 cut(s)	73
AcsI	RAATTY	2 cut(s)	383, 574
AcvI	CACGTG	2 cut(s)	583, 685
AcyI	GRCGYC	3 cut(s)	9, 183, 321
AfaI	GTAC	4 cut(s)	269, 452, 506, 953
AfiI	CCNNNNNNNGG	1 cut(s)	447
AflIII	ACRYGT	2 cut(s)	264, 582
AhdI	GACNNNNNGTC	1 cut(s)	187
AjnI	CCWGG	2 cut(s)	174, 486
AluBI	AGCT	8 cut(s)	112, 160, 349, 793, 868, 879, 886, 948
AluI	AGCT	8 cut(s)	112, 160, 349, 793, 868, 879, 886, 948
Alw21I	GWGCWC	1 cut(s)	162
AlwI	GGATC	3 cut(s)	217, 295, 482
AoxI	GGCC	5 cut(s)	73, 82, 177, 548, 820
ApoI	RAATTY	2 cut(s)	383, 574
Asp700I	GAANNNNTTC	1 cut(s)	62
AspA2I	CCTAGG	1 cut(s)	121
AspLEI	GCGC	1 cut(s)	11
AspS9I	GGNCC	3 cut(s)	425, 548, 820
AsuC2I	CCSGG	3 cut(s)	272, 404, 472
AsuHPI	GGTGA	3 cut(s)	121, 557, 634
AvaII	GGWCC	1 cut(s)	425
AvrII	CCTAGG	1 cut(s)	121
BanI	GGYRCC	1 cut(s)	8
BanII	GRGCYC	1 cut(s)	162
BauI	CACGAG	1 cut(s)	611
BbrPI	CACGTG	2 cut(s)	583, 685
Bbv12I	GWGCWC	1 cut(s)	162
BccI	CCATC	6 cut(s)	107, 407, 464, 695, 712, 845
BceAI	ACGGC	1 cut(s)	272
BciT130I	CCWGG	2 cut(s)	176, 488
BcnI	CCSGG	3 cut(s)	272, 404, 472
BfaI	CTAG	1 cut(s)	122
BfoI	RGCGCY	1 cut(s)	12
BglI	GCCNNNNNGGC	2 cut(s)	81, 90
BglII	AGATCT	1 cut(s)	912
BisI	GCNGC	6 cut(s)	82, 201, 231, 234, 324, 551
BlnI	CCTAGG	1 cut(s)	121
BlpI	GCTNAGC	1 cut(s)	789
BlsI	GCNGC	6 cut(s)	83, 202, 232, 235, 325, 552
Bme1390I	CCNGG	5 cut(s)	176, 272, 404, 472, 488
Bme18I	GGWCC	1 cut(s)	425
BmeRI	GACNNNNNGTC	1 cut(s)	187
BmgT120I	GGNCC	3 cut(s)	425, 548, 820
BmiI	GGNNCC	5 cut(s)	10, 94, 364, 426, 435
BmrFI	CCNGG	5 cut(s)	176, 272, 404, 472, 488
BmsI	GCATC	2 cut(s)	505, 519
BoxI	GACNNNNGTC	1 cut(s)	311
BpmI	CTGGAG	2 cut(s)	281, 452
Bpu1102I	GCTNAGC	1 cut(s)	789
BpuMI	CCSGG	3 cut(s)	272, 404, 472
BsaAI	YACGTR	3 cut(s)	267, 583, 685
BsaHI	GRCGYC	3 cut(s)	9, 183, 321
BsaJI	CCNNGG	4 cut(s)	79, 121, 358, 860
Bsc4I	CCNNNNNNNGG	1 cut(s)	447
Bse118I	RCCGGY	2 cut(s)	71, 589
Bse1I	ACTGG	2 cut(s)	435, 661
Bse3DI	GCAATG	1 cut(s)	413
BseBI	CCWGG	2 cut(s)	176, 488
BseDI	CCNNGG	4 cut(s)	79, 121, 358, 860
BseGI	GGATG	3 cut(s)	399, 520, 534
BseLI	CCNNNNNNNGG	1 cut(s)	447
BseMI	GCAATG	1 cut(s)	413
BseMII	CTCAG	2 cut(s)	659, 780
BseNI	ACTGG	2 cut(s)	435, 661
BseRI	GAGGAG	3 cut(s)	355, 381, 650
Bsh1236I	CGCG	1 cut(s)	81
BshFI	GGCC	5 cut(s)	75, 84, 179, 550, 822
BshNI	GGYRCC	1 cut(s)	8
BsiHKAI	GWGCWC	1 cut(s)	162
BsiSI	CCGG	5 cut(s)	72, 272, 404, 471, 590
BslFI	GGGAC	3 cut(s)	438, 667, 848
BslI	CCNNNNNNNGG	1 cut(s)	447
BsmFI	GGGAC	3 cut(s)	438, 667, 848
BsmI	GAATGC	1 cut(s)	249
BsnI	GGCC	5 cut(s)	75, 84, 179, 550, 822
Bsp1286I	GDGCHC	1 cut(s)	162
Bsp143I	GATC	5 cut(s)	222, 300, 465, 474, 912
Bsp1720I	GCTNAGC	1 cut(s)	789
BspANI	GGCC	5 cut(s)	75, 84, 179, 550, 822
BspCNI	CTCAG	2 cut(s)	660, 781
BspFNI	CGCG	1 cut(s)	81
BspHI	TCATGA	1 cut(s)	714
BspLI	GGNNCC	5 cut(s)	10, 94, 364, 426, 435
BspPI	GGATC	3 cut(s)	217, 295, 482
BspT107I	GGYRCC	1 cut(s)	8
BsrDI	GCAATG	1 cut(s)	413
BsrFI	RCCGGY	2 cut(s)	71, 589
BsrI	ACTGG	2 cut(s)	435, 661
BssAI	RCCGGY	2 cut(s)	71, 589
BssECI	CCNNGG	4 cut(s)	79, 121, 358, 860
BssMI	GATC	5 cut(s)	222, 300, 465, 474, 912
BssNI	GRCGYC	3 cut(s)	9, 183, 321
BssSI	CACGAG	1 cut(s)	611
BssT1I	CCWWGG	2 cut(s)	121, 358
Bst2BI	CACGAG	1 cut(s)	611
Bst2UI	CCWGG	2 cut(s)	176, 488
Bst4CI	ACNGT	2 cut(s)	509, 725
BstACI	GRCGYC	3 cut(s)	9, 183, 321
BstAPI	GCANNNNNTGC	1 cut(s)	586
BstBAI	YACGTR	3 cut(s)	267, 583, 685
BstC8I	GCNNGC	4 cut(s)	73, 110, 591, 888
BstDEI	CTNAG	2 cut(s)	668, 789
BstDSI	CCRYGG	1 cut(s)	79
BstF5I	GGATG	3 cut(s)	399, 520, 534
BstFNI	CGCG	1 cut(s)	81
BstH2I	RGCGCY	1 cut(s)	12
BstHHI	GCGC	1 cut(s)	11
BstKTI	GATC	5 cut(s)	225, 303, 468, 477, 915
BstMBI	GATC	5 cut(s)	222, 300, 465, 474, 912
BstMWI	GCNNNNNNNGC	6 cut(s)	81, 90, 132, 369, 467, 586
BstNI	CCWGG	2 cut(s)	176, 488
BstNSI	RCATGY	1 cut(s)	746
BstPAI	GACNNNNGTC	1 cut(s)	311
BstSCI	CCNGG	5 cut(s)	174, 270, 402, 470, 486
BstUI	CGCG	1 cut(s)	81
BstX2I	RGATCY	2 cut(s)	300, 912
BstYI	RGATCY	2 cut(s)	300, 912
BsuRI	GGCC	5 cut(s)	75, 84, 179, 550, 822
BtgI	CCRYGG	1 cut(s)	79
BtgZI	GCGATG	2 cut(s)	268, 442
BtsCI	GGATG	3 cut(s)	399, 520, 534
BtsIMutI	CAGTG	1 cut(s)	428
Cac8I	GCNNGC	4 cut(s)	73, 110, 591, 888
CciI	TCATGA	1 cut(s)	714
CfoI	GCGC	1 cut(s)	11
Cfr10I	RCCGGY	2 cut(s)	71, 589
Cfr13I	GGNCC	3 cut(s)	425, 548, 820
Cfr42I	CCGCGG	1 cut(s)	82
CseI	GACGC	1 cut(s)	329
Csp6I	GTAC	4 cut(s)	268, 451, 505, 952
CviAII	CATG	5 cut(s)	116, 554, 715, 743, 871
CviQI	GTAC	4 cut(s)	268, 451, 505, 952
DdeI	CTNAG	2 cut(s)	668, 789
DinI	GGCGCC	1 cut(s)	10
DpnI	GATC	5 cut(s)	224, 302, 467, 476, 914
DpnII	GATC	5 cut(s)	222, 300, 465, 474, 912
DriI	GACNNNNNGTC	1 cut(s)	187
EaeI	YGGCCR	1 cut(s)	73
Eam1105I	GACNNNNNGTC	1 cut(s)	187
EciI	GGCGGA	3 cut(s)	85, 290, 647
Ecl136II	GAGCTC	1 cut(s)	160
Eco130I	CCWWGG	2 cut(s)	121, 358
Eco24I	GRGCYC	1 cut(s)	162
Eco47I	GGWCC	1 cut(s)	425
Eco53kI	GAGCTC	1 cut(s)	160
Eco72I	CACGTG	2 cut(s)	583, 685
EcoICRI	GAGCTC	1 cut(s)	160
EcoRII	CCWGG	2 cut(s)	174, 486
EcoT14I	CCWWGG	2 cut(s)	121, 358
EcoT38I	GRGCYC	1 cut(s)	162
EgeI	GGCGCC	1 cut(s)	10
EheI	GGCGCC	1 cut(s)	10
ErhI	CCWWGG	2 cut(s)	121, 358
FaeI	CATG	5 cut(s)	119, 557, 718, 746, 874
FaiI	YATR	9 cut(s)	117, 317, 546, 555, 716, 729, 744, 872, 945
FalI	AAGNNNNNCTT	4 cut(s)	335, 367, 915, 947
FaqI	GGGAC	3 cut(s)	438, 667, 848
FatI	CATG	5 cut(s)	115, 553, 714, 742, 870
Fnu4HI	GCNGC	6 cut(s)	82, 201, 231, 234, 324, 551
FokI	GGATG	3 cut(s)	386, 527, 541
FriOI	GRGCYC	1 cut(s)	162
Fsp4HI	GCNGC	6 cut(s)	82, 201, 231, 234, 324, 551
FspBI	CTAG	1 cut(s)	122
GlaI	GCGC	1 cut(s)	10
GluI	GCNGC	6 cut(s)	82, 201, 231, 234, 324, 551
GsuI	CTGGAG	2 cut(s)	281, 452
HaeII	RGCGCY	1 cut(s)	12
HaeIII	GGCC	5 cut(s)	75, 84, 179, 550, 822
HapII	CCGG	5 cut(s)	72, 272, 404, 471, 590
HgaI	GACGC	1 cut(s)	329
HhaI	GCGC	1 cut(s)	11
Hin1I	GRCGYC	3 cut(s)	9, 183, 321
Hin1II	CATG	5 cut(s)	119, 557, 718, 746, 874
Hin6I	GCGC	1 cut(s)	9
HinP1I	GCGC	1 cut(s)	9
HindIII	AAGCTT	1 cut(s)	884
HinfI	GANTC	4 cut(s)	188, 339, 499, 836
HpaII	CCGG	5 cut(s)	72, 272, 404, 471, 590
HphI	GGTGA	3 cut(s)	121, 557, 634
Hpy166II	GTNNAC	2 cut(s)	688, 903
Hpy188I	TCNGA	3 cut(s)	46, 498, 861
Hpy188III	TCNNGA	7 cut(s)	220, 298, 304, 343, 478, 611, 715
Hpy8I	GTNNAC	2 cut(s)	688, 903
Hpy99I	CGWCG	3 cut(s)	92, 185, 188
HpyAV	CCTTC	3 cut(s)	42, 46, 52
HpyCH4III	ACNGT	2 cut(s)	509, 725
HpyCH4IV	ACGT	4 cut(s)	183, 266, 582, 684
HpyCH4V	TGCA	4 cut(s)	372, 518, 682, 890
HpyF10VI	GCNNNNNNNGC	6 cut(s)	81, 90, 132, 369, 467, 586
HpyF3I	CTNAG	2 cut(s)	668, 789
HpySE526I	ACGT	4 cut(s)	183, 266, 582, 684
Hsp92I	GRCGYC	3 cut(s)	9, 183, 321
Hsp92II	CATG	5 cut(s)	119, 557, 718, 746, 874
HspAI	GCGC	1 cut(s)	9
KasI	GGCGCC	1 cut(s)	8
KroI	GCCGGC	2 cut(s)	71, 589
KroNI	GCCGGC	2 cut(s)	73, 591
KspI	CCGCGG	1 cut(s)	82
Kzo9I	GATC	5 cut(s)	222, 300, 465, 474, 912
LmnI	GCTCC	6 cut(s)	98, 117, 368, 394, 433, 739
LweI	GCATC	2 cut(s)	505, 519
MaeI	CTAG	1 cut(s)	122
MaeII	ACGT	4 cut(s)	183, 266, 582, 684
MaeIII	GTNAC	1 cut(s)	769
MalI	GATC	5 cut(s)	224, 302, 467, 476, 914
MboI	GATC	5 cut(s)	222, 300, 465, 474, 912
MboII	GAAGA	1 cut(s)	922
MflI	RGATCY	2 cut(s)	300, 912
MhlI	GDGCHC	1 cut(s)	162
MluCI	AATT	6 cut(s)	215, 383, 519, 538, 574, 774
Mly113I	GGCGCC	1 cut(s)	9
MlyI	GAGTC	3 cut(s)	197, 348, 830
MmeI	TCCRAC	1 cut(s)	717
MroNI	GCCGGC	2 cut(s)	71, 589
MroXI	GAANNNNTTC	1 cut(s)	62
MseI	TTAA	1 cut(s)	777
MslI	CAYNNNNRTG	1 cut(s)	747
MspA1I	CMGCKG	1 cut(s)	81
MspI	CCGG	5 cut(s)	72, 272, 404, 471, 590
MspR9I	CCNGG	5 cut(s)	176, 272, 404, 472, 488
Mva1269I	GAATGC	1 cut(s)	249
MvaI	CCWGG	2 cut(s)	176, 488
MvnI	CGCG	1 cut(s)	81
MwoI	GCNNNNNNNGC	6 cut(s)	81, 90, 132, 369, 467, 586
NaeI	GCCGGC	2 cut(s)	73, 591
NarI	GGCGCC	1 cut(s)	9
NciI	CCSGG	3 cut(s)	272, 404, 472
NdeII	GATC	5 cut(s)	222, 300, 465, 474, 912
NgoMIV	GCCGGC	2 cut(s)	71, 589
NlaIII	CATG	5 cut(s)	119, 557, 718, 746, 874
NlaIV	GGNNCC	5 cut(s)	10, 94, 364, 426, 435
NmeAIII	GCCGAG	2 cut(s)	181, 229
NmuCI	GTSAC	1 cut(s)	769
NspI	RCATGY	1 cut(s)	746
PagI	TCATGA	1 cut(s)	714
PcsI	WCGNNNNNNNCGW	1 cut(s)	159
PctI	GAATGC	1 cut(s)	249
PdiI	GCCGGC	2 cut(s)	73, 591
PdmI	GAANNNNTTC	1 cut(s)	62
PfeI	GAWTC	1 cut(s)	499
PflMI	CCANNNNNTGG	1 cut(s)	447
PfoI	TCCNGGA	1 cut(s)	402
PkrI	GCNGC	6 cut(s)	83, 202, 232, 235, 325, 552
PleI	GAGTC	3 cut(s)	196, 347, 830
PluTI	GGCGCC	1 cut(s)	12
PmaCI	CACGTG	2 cut(s)	583, 685
PmlI	CACGTG	2 cut(s)	583, 685
PpsI	GAGTC	3 cut(s)	196, 347, 830
Ppu21I	YACGTR	3 cut(s)	267, 583, 685
PshAI	GACNNNNGTC	1 cut(s)	311
Psp124BI	GAGCTC	1 cut(s)	162
Psp6I	CCWGG	2 cut(s)	174, 486
PspCI	CACGTG	2 cut(s)	583, 685
PspGI	CCWGG	2 cut(s)	174, 486
PspN4I	GGNNCC	5 cut(s)	10, 94, 364, 426, 435
PspPI	GGNCC	3 cut(s)	425, 548, 820
PsuI	RGATCY	2 cut(s)	300, 912
RsaI	GTAC	4 cut(s)	269, 452, 506, 953
RsaNI	GTAC	4 cut(s)	268, 451, 505, 952
RseI	CAYNNNNRTG	1 cut(s)	747
SacI	GAGCTC	1 cut(s)	162
SacII	CCGCGG	1 cut(s)	82
SaqAI	TTAA	1 cut(s)	777
SatI	GCNGC	6 cut(s)	82, 201, 231, 234, 324, 551
Sau3AI	GATC	5 cut(s)	222, 300, 465, 474, 912
Sau96I	GGNCC	3 cut(s)	425, 548, 820
SchI	GAGTC	3 cut(s)	197, 348, 830
ScrFI	CCNGG	5 cut(s)	176, 272, 404, 472, 488
SduI	GDGCHC	1 cut(s)	162
SfaNI	GCATC	2 cut(s)	505, 519
SfiI	GGCCNNNNNGGCC	1 cut(s)	81
SfoI	GGCGCC	1 cut(s)	10
Sfr303I	CCGCGG	1 cut(s)	82
SgrBI	CCGCGG	1 cut(s)	82
SinI	GGWCC	1 cut(s)	425
SmiMI	CAYNNNNRTG	1 cut(s)	747
Sse9I	AATT	6 cut(s)	215, 383, 519, 538, 574, 774
SspDI	GGCGCC	1 cut(s)	8
SspMI	CTAG	1 cut(s)	122
SstI	GAGCTC	1 cut(s)	162
StyD4I	CCNGG	5 cut(s)	174, 270, 402, 470, 486
StyI	CCWWGG	2 cut(s)	121, 358
TaaI	ACNGT	2 cut(s)	509, 725
TaiI	ACGT	4 cut(s)	186, 269, 585, 687
TaqI	TCGA	3 cut(s)	162, 186, 816
TasI	AATT	6 cut(s)	215, 383, 519, 538, 574, 774
TatI	WGTACW	1 cut(s)	504
TauI	GCSGC	6 cut(s)	84, 203, 233, 236, 326, 553
TfiI	GAWTC	1 cut(s)	499
Tru1I	TTAA	1 cut(s)	777
Tru9I	TTAA	1 cut(s)	777
TscAI	CASTG	1 cut(s)	435
TseFI	GTSAC	1 cut(s)	769
Tsp45I	GTSAC	1 cut(s)	769
TspDTI	ATGAA	2 cut(s)	271, 703
TspRI	CASTG	1 cut(s)	435
Van91I	CCANNNNNTGG	1 cut(s)	447
VpaK11BI	GGWCC	1 cut(s)	425
XapI	RAATTY	2 cut(s)	383, 574
XceI	RCATGY	1 cut(s)	746
XmaJI	CCTAGG	1 cut(s)	121
XmnI	GAANNNNTTC	1 cut(s)	62
XspI	CTAG	1 cut(s)	122
ZraI	GACGTC	1 cut(s)	184

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.