RchiOBHm_Chr4g0405311

GDP-L-fucose synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0010988
Physical Location & Seq
Forward (+)
25616222 .. 25617654
1433 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ37680

Sequence Viewer

Length: 975 bp
ATGAGTAGCGCCATTAACGATGCCACCTCGCCTTCCTTCTTCTCCAACAAATCCGCAAAGATCTTCGTCGCCGGCCACCGCGGCCTGGTGGGCTCCGCCATCGTCCGCAAGCTCCAATCCCTAGGCTTCACCAACCTCGTCCTCCGCACCCACGCCGACCTCGACCTCACGCGCCAGAACGACGTCGAGTCCTTCTTCGCCGCCGAGAAGCCCCAATTCGTAATCCTCGCCGCGGCTAAAGTGGGCGGCATCCACGCCAACAACACCTACCCGGCTGACTTCATCTCCCTCAACCTCCAAATCCAGACGAACGTCATCGACGCCGCGCACCGATTCGGAGTCACCAAGCTCCTCTTCCTCGGCTCCTCTTGCATTTACCCCAAGTTCGCGCCCCAGCCCATCCCTGAATCCGCGCTCTTAACGGGCCCACTCGAGCCCACCAACGAGTGGTACGCCATTGCCAAGATCGCCGGCATCAAGATGTGCCAGGCTTACCGGATTCAGCACAACTGGGACGCCATTTCCGGCATGCCTACCAACCTGTATGGCCCGCACGACAACTTCCACCCCGAGAATTCGCACGTGCTGCCGGCATTGATGAGGCGATTTCACGAGGCCAAGGTCAATGGTGCCAAAGAGGTGGTGGTGTGGGGGACTGGAAGCCCCTTGAGGGAGTTTCTGCACGTGGATGATCTCGCCGATGCGGTGGTGTTCTTGATGGGAAGTTACAGTGGGTTGGAGCATGTCAATGTGGGTAGTGGAAAGGAGGTCACTATTAAGGAGCTGGCGGAATTGGTCAAGGAAGTTGTGGGGTTTCAAGGTCAGCTTGTTTGGGACTCGTCCAAGCCCGATGGAACTCCCAGGAAGCTCATGGATAGCTCCAAGCTCGCCGGATTGGGTTGGACCCCGAAGATCTCACTCAAGGAAGGGCTGGTTGGTACCTACAAGTGGTACTTGGAGAATGTCAAGCAATGA

Protein Analysis

324

Amino Acids

35.5

Weight (kDa)

7.81

Isoelectric Point (pI)

23.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RmlD_sub_bind PF04321 20 - 138 1.5e-07 RmlD substrate binding domain
Epimerase PF01370 21 - 252 4.5e-64 NAD dependent epimerase/dehydratase family
GDP_Man_Dehyd PF16363 53 - 311 5.2e-18 GDP-mannose 4,6 dehydratase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010988)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G17890 AT1G17890 AT1G17890 AT1G73250
fragaria_vesca FvH4_4g08850 FvH4_4g08850 FvH4_4g08850
malus_domestica MD05G1099800.v1.1 MD10G1105500.v1.1
prunus_persica Prupe.1G100900_v2.0.a1
pyrus_communis pycom05g09860 pycom10g09090
rosa_chinensis RchiOBHm_Chr4g0405311
rosa_laevigata RLG00000008852
rosa_multiflora Rmu_sc0000161.1_g000028
rosa_roxburghii Rroxscaffold_5G00349690
rosa_rugosa Rorug04G0057300
rosa_samantha Rh4BG125000 Rh4CG137600
rosa_wichuraiana Rw4G010590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 186
Acc65I GGTACC 1 cut(s) 938
AccB1I GGYRCC 2 cut(s) 629, 938
AccB7I CCANNNNNTGG 1 cut(s) 447
AccII CGCG 6 cut(s) 81, 172, 233, 326, 389, 413
AcoI YGGCCR 1 cut(s) 73
AcsI RAATTY 1 cut(s) 574
AcvI CACGTG 2 cut(s) 583, 685
AcyI GRCGYC 3 cut(s) 183, 321, 516
AfaI GTAC 3 cut(s) 452, 940, 953
AfiI CCNNNNNNNGG 5 cut(s) 85, 232, 447, 670, 948
AgsI TTSAA 1 cut(s) 818
AhdI GACNNNNNGTC 1 cut(s) 187
AjnI CCWGG 3 cut(s) 84, 486, 860
AjuI GAANNNNNNNTTGG 4 cut(s) 338, 370, 918, 950
AluBI AGCT 7 cut(s) 112, 349, 784, 826, 868, 879, 886
AluI AGCT 7 cut(s) 112, 349, 784, 826, 868, 879, 886
Ama87I CYCGRG 2 cut(s) 431, 569
AoxI GGCC 5 cut(s) 73, 82, 424, 547, 615
ApaI GGGCCC 1 cut(s) 428
ApeKI GCWGC 1 cut(s) 586
ApoI RAATTY 1 cut(s) 574
Asp718I GGTACC 1 cut(s) 938
AspA2I CCTAGG 1 cut(s) 121
AspLEI GCGC 5 cut(s) 11, 174, 328, 391, 415
AspS9I GGNCC 4 cut(s) 424, 425, 548, 903
AsuC2I CCSGG 1 cut(s) 272
AsuHPI GGTGA 2 cut(s) 121, 334
AvaI CYCGRG 2 cut(s) 431, 569
AvaII GGWCC 1 cut(s) 903
AvrII CCTAGG 1 cut(s) 121
BaeGI GKGCMC 1 cut(s) 428
BanI GGYRCC 2 cut(s) 629, 938
BanII GRGCYC 3 cut(s) 95, 428, 438
BauI CACGAG 1 cut(s) 611
BbrPI CACGTG 2 cut(s) 583, 685
BbvI GCAGC 1 cut(s) 573
BccI CCATC 4 cut(s) 107, 407, 712, 845
BciT130I CCWGG 3 cut(s) 86, 488, 862
BcnI CCSGG 1 cut(s) 272
BfaI CTAG 1 cut(s) 122
BfoI RGCGCY 1 cut(s) 12
BglI GCCNNNNNGGC 2 cut(s) 81, 90
BglII AGATCT 2 cut(s) 60, 912
BisI GCNGC 7 cut(s) 82, 201, 231, 234, 247, 324, 587
BlnI CCTAGG 1 cut(s) 121
BlsI GCNGC 7 cut(s) 83, 202, 232, 235, 248, 325, 588
Bme1390I CCNGG 4 cut(s) 86, 272, 488, 862
Bme18I GGWCC 1 cut(s) 903
BmeRI GACNNNNNGTC 1 cut(s) 187
BmeT110I CYCGRG 2 cut(s) 431, 569
BmgT120I GGNCC 4 cut(s) 424, 425, 548, 903
BmiI GGNNCC 6 cut(s) 94, 364, 426, 631, 905, 940
BmrFI CCNGG 4 cut(s) 86, 272, 488, 862
BmrI ACTGGG 1 cut(s) 520
BmsI GCATC 4 cut(s) 10, 258, 483, 691
BmuI ACTGGG 1 cut(s) 520
BoxI GACNNNNGTC 1 cut(s) 311
BpuEI CTTGAG 2 cut(s) 688, 905
BpuMI CCSGG 1 cut(s) 272
BsaAI YACGTR 2 cut(s) 583, 685
BsaHI GRCGYC 3 cut(s) 183, 321, 516
BsaJI CCNNGG 6 cut(s) 79, 121, 231, 358, 618, 860
BsaWI WCCGGW 1 cut(s) 495
BsaXI ACNNNNNCTCC 2 cut(s) 269, 299
Bsc4I CCNNNNNNNGG 5 cut(s) 85, 232, 447, 670, 948
Bse118I RCCGGY 3 cut(s) 71, 470, 589
Bse1I ACTGG 2 cut(s) 515, 661
Bse3DI GCAATG 1 cut(s) 456
BseBI CCWGG 3 cut(s) 86, 488, 862
BseDI CCNNGG 6 cut(s) 79, 121, 231, 358, 618, 860
BseGI GGATG 3 cut(s) 249, 399, 694
BseLI CCNNNNNNNGG 5 cut(s) 85, 232, 447, 670, 948
BseMI GCAATG 1 cut(s) 456
BseNI ACTGG 2 cut(s) 515, 661
BseRI GAGGAG 2 cut(s) 341, 355
BseSI GKGCMC 1 cut(s) 428
BseXI GCAGC 1 cut(s) 573
BseYI CCCAGC 1 cut(s) 393
BsgI GTGCAG 1 cut(s) 665
Bsh1236I CGCG 6 cut(s) 81, 172, 233, 326, 389, 413
BshFI GGCC 5 cut(s) 75, 84, 426, 549, 617
BshNI GGYRCC 2 cut(s) 629, 938
BsiHKCI CYCGRG 2 cut(s) 431, 569
BsiSI CCGG 7 cut(s) 72, 272, 471, 496, 525, 590, 891
BslFI GGGAC 3 cut(s) 527, 667, 848
BslI CCNNNNNNNGG 5 cut(s) 85, 232, 447, 670, 948
BsmFI GGGAC 3 cut(s) 527, 667, 848
BsnI GGCC 5 cut(s) 75, 84, 426, 549, 617
BsoBI CYCGRG 2 cut(s) 431, 569
Bsp120I GGGCCC 1 cut(s) 424
Bsp1286I GDGCHC 3 cut(s) 95, 428, 438
Bsp143I GATC 4 cut(s) 60, 465, 691, 912
BspANI GGCC 5 cut(s) 75, 84, 426, 549, 617
BspFNI CGCG 6 cut(s) 81, 172, 233, 326, 389, 413
BspLI GGNNCC 6 cut(s) 94, 364, 426, 631, 905, 940
BspT107I GGYRCC 2 cut(s) 629, 938
BsrDI GCAATG 1 cut(s) 456
BsrFI RCCGGY 3 cut(s) 71, 470, 589
BsrI ACTGG 2 cut(s) 515, 661
BssAI RCCGGY 3 cut(s) 71, 470, 589
BssECI CCNNGG 6 cut(s) 79, 121, 231, 358, 618, 860
BssMI GATC 4 cut(s) 60, 465, 691, 912
BssNI GRCGYC 3 cut(s) 183, 321, 516
BssSI CACGAG 1 cut(s) 611
BssT1I CCWWGG 2 cut(s) 121, 618
Bst2BI CACGAG 1 cut(s) 611
Bst2UI CCWGG 3 cut(s) 86, 488, 862
Bst4CI ACNGT 1 cut(s) 731
Bst6I CTCTTC 1 cut(s) 359
BstACI GRCGYC 3 cut(s) 183, 321, 516
BstAPI GCANNNNNTGC 1 cut(s) 586
BstBAI YACGTR 2 cut(s) 583, 685
BstC8I GCNNGC 8 cut(s) 73, 110, 472, 530, 551, 591, 786, 888
BstDSI CCRYGG 2 cut(s) 79, 231
BstF5I GGATG 3 cut(s) 249, 399, 694
BstFNI CGCG 6 cut(s) 81, 172, 233, 326, 389, 413
BstH2I RGCGCY 1 cut(s) 12
BstHHI GCGC 5 cut(s) 11, 174, 328, 391, 415
BstKTI GATC 4 cut(s) 63, 468, 694, 915
BstMBI GATC 4 cut(s) 60, 465, 691, 912
BstMWI GCNNNNNNNGC 5 cut(s) 81, 90, 369, 467, 586
BstNI CCWGG 3 cut(s) 86, 488, 862
BstNSI RCATGY 2 cut(s) 532, 746
BstPAI GACNNNNGTC 1 cut(s) 311
BstSCI CCNGG 4 cut(s) 84, 270, 486, 860
BstSLI GKGCMC 1 cut(s) 428
BstUI CGCG 6 cut(s) 81, 172, 233, 326, 389, 413
BstV1I GCAGC 1 cut(s) 573
BstX2I RGATCY 2 cut(s) 60, 912
BstXI CCANNNNNNTGG 1 cut(s) 640
BstYI RGATCY 2 cut(s) 60, 912
BsuRI GGCC 5 cut(s) 75, 84, 426, 549, 617
BtgI CCRYGG 2 cut(s) 79, 231
BtsCI GGATG 3 cut(s) 249, 399, 694
BtsIMutI CAGTG 1 cut(s) 736
Cac8I GCNNGC 8 cut(s) 73, 110, 472, 530, 551, 591, 786, 888
CfoI GCGC 5 cut(s) 11, 174, 328, 391, 415
Cfr10I RCCGGY 3 cut(s) 71, 470, 589
Cfr13I GGNCC 4 cut(s) 424, 425, 548, 903
Cfr42I CCGCGG 2 cut(s) 82, 234
CseI GACGC 2 cut(s) 329, 524
Csp6I GTAC 3 cut(s) 451, 939, 952
CviAII CATG 3 cut(s) 529, 743, 871
CviQI GTAC 3 cut(s) 451, 939, 952
DpnI GATC 4 cut(s) 62, 467, 693, 914
DpnII GATC 4 cut(s) 60, 465, 691, 912
DriI GACNNNNNGTC 1 cut(s) 187
EaeI YGGCCR 1 cut(s) 73
Eam1104I CTCTTC 1 cut(s) 359
Eam1105I GACNNNNNGTC 1 cut(s) 187
EarI CTCTTC 1 cut(s) 359
EciI GGCGGA 2 cut(s) 85, 803
Eco130I CCWWGG 2 cut(s) 121, 618
Eco24I GRGCYC 3 cut(s) 95, 428, 438
Eco47I GGWCC 1 cut(s) 903
Eco72I CACGTG 2 cut(s) 583, 685
Eco88I CYCGRG 2 cut(s) 431, 569
EcoRI GAATTC 1 cut(s) 574
EcoRII CCWGG 3 cut(s) 84, 486, 860
EcoT14I CCWWGG 2 cut(s) 121, 618
EcoT38I GRGCYC 3 cut(s) 95, 428, 438
ErhI CCWWGG 2 cut(s) 121, 618
FaeI CATG 3 cut(s) 532, 746, 874
FaiI YATR 4 cut(s) 530, 546, 744, 872
FalI AAGNNNNNCTT 6 cut(s) 338, 370, 810, 842, 938, 970
FaqI GGGAC 3 cut(s) 527, 667, 848
FatI CATG 3 cut(s) 528, 742, 870
FauI CCCGC 1 cut(s) 558
Fnu4HI GCNGC 7 cut(s) 82, 201, 231, 234, 247, 324, 587
FokI GGATG 3 cut(s) 236, 386, 701
FriOI GRGCYC 3 cut(s) 95, 428, 438
Fsp4HI GCNGC 7 cut(s) 82, 201, 231, 234, 247, 324, 587
FspBI CTAG 1 cut(s) 122
GlaI GCGC 5 cut(s) 10, 173, 327, 390, 414
GluI GCNGC 7 cut(s) 82, 201, 231, 234, 247, 324, 587
GsaI CCCAGC 1 cut(s) 397
HaeII RGCGCY 1 cut(s) 12
HaeIII GGCC 5 cut(s) 75, 84, 426, 549, 617
HapII CCGG 7 cut(s) 72, 272, 471, 496, 525, 590, 891
HgaI GACGC 2 cut(s) 329, 524
HhaI GCGC 5 cut(s) 11, 174, 328, 391, 415
Hin1I GRCGYC 3 cut(s) 183, 321, 516
Hin1II CATG 3 cut(s) 532, 746, 874
Hin6I GCGC 5 cut(s) 9, 172, 326, 389, 413
HinP1I GCGC 5 cut(s) 9, 172, 326, 389, 413
HinfI GANTC 6 cut(s) 188, 333, 339, 407, 499, 836
HpaII CCGG 7 cut(s) 72, 272, 471, 496, 525, 590, 891
HphI GGTGA 2 cut(s) 121, 334
Hpy188I TCNGA 1 cut(s) 338
Hpy188III TCNNGA 4 cut(s) 304, 478, 611, 715
Hpy99I CGWCG 4 cut(s) 71, 185, 188, 323
HpyAV CCTTC 4 cut(s) 42, 46, 202, 920
HpyCH4III ACNGT 1 cut(s) 731
HpyCH4IV ACGT 4 cut(s) 183, 312, 582, 684
HpyCH4V TGCA 2 cut(s) 372, 682
HpyF10VI GCNNNNNNNGC 5 cut(s) 81, 90, 369, 467, 586
HpySE526I ACGT 4 cut(s) 183, 312, 582, 684
Hsp92I GRCGYC 3 cut(s) 183, 321, 516
Hsp92II CATG 3 cut(s) 532, 746, 874
HspAI GCGC 5 cut(s) 9, 172, 326, 389, 413
KpnI GGTACC 1 cut(s) 942
KroI GCCGGC 3 cut(s) 71, 470, 589
KroNI GCCGGC 3 cut(s) 73, 472, 591
KspI CCGCGG 2 cut(s) 82, 234
Kzo9I GATC 4 cut(s) 60, 465, 691, 912
LmnI GCTCC 7 cut(s) 98, 117, 354, 368, 739, 781, 884
Lsp1109I GCAGC 1 cut(s) 573
LweI GCATC 4 cut(s) 10, 258, 483, 691
MaeI CTAG 1 cut(s) 122
MaeII ACGT 4 cut(s) 183, 312, 582, 684
MaeIII GTNAC 3 cut(s) 340, 725, 769
MalI GATC 4 cut(s) 62, 467, 693, 914
MboI GATC 4 cut(s) 60, 465, 691, 912
MboII GAAGA 5 cut(s) 31, 55, 187, 346, 922
MflI RGATCY 2 cut(s) 60, 912
MhlI GDGCHC 3 cut(s) 95, 428, 438
MluCI AATT 3 cut(s) 215, 574, 791
MlyI GAGTC 3 cut(s) 197, 348, 830
MmeI TCCRAC 3 cut(s) 69, 717, 881
MroNI GCCGGC 3 cut(s) 71, 470, 589
MseI TTAA 3 cut(s) 15, 419, 777
MslI CAYNNNNRTG 3 cut(s) 479, 687, 747
MspA1I CMGCKG 2 cut(s) 81, 233
MspI CCGG 7 cut(s) 72, 272, 471, 496, 525, 590, 891
MspR9I CCNGG 4 cut(s) 86, 272, 488, 862
MvaI CCWGG 3 cut(s) 86, 488, 862
MvnI CGCG 6 cut(s) 81, 172, 233, 326, 389, 413
MwoI GCNNNNNNNGC 5 cut(s) 81, 90, 369, 467, 586
NaeI GCCGGC 3 cut(s) 73, 472, 591
NciI CCSGG 1 cut(s) 272
NdeII GATC 4 cut(s) 60, 465, 691, 912
NgoMIV GCCGGC 3 cut(s) 71, 470, 589
NlaIII CATG 3 cut(s) 532, 746, 874
NlaIV GGNNCC 6 cut(s) 94, 364, 426, 631, 905, 940
NmeAIII GCCGAG 2 cut(s) 229, 339
NmuCI GTSAC 2 cut(s) 340, 769
NspI RCATGY 2 cut(s) 532, 746
PaeI GCATGC 1 cut(s) 532
PaeR7I CTCGAG 1 cut(s) 431
PcsI WCGNNNNNNNCGW 1 cut(s) 159
PdiI GCCGGC 3 cut(s) 73, 472, 591
PfeI GAWTC 3 cut(s) 333, 407, 499
PflMI CCANNNNNTGG 1 cut(s) 447
PkrI GCNGC 7 cut(s) 83, 202, 232, 235, 248, 325, 588
PleI GAGTC 3 cut(s) 196, 347, 830
PmaCI CACGTG 2 cut(s) 583, 685
PmlI CACGTG 2 cut(s) 583, 685
PpsI GAGTC 3 cut(s) 196, 347, 830
Ppu21I YACGTR 2 cut(s) 583, 685
PshAI GACNNNNGTC 1 cut(s) 311
Psp6I CCWGG 3 cut(s) 84, 486, 860
PspCI CACGTG 2 cut(s) 583, 685
PspFI CCCAGC 1 cut(s) 393
PspGI CCWGG 3 cut(s) 84, 486, 860
PspN4I GGNNCC 6 cut(s) 94, 364, 426, 631, 905, 940
PspOMI GGGCCC 1 cut(s) 424
PspPI GGNCC 4 cut(s) 424, 425, 548, 903
PspXI VCTCGAGB 1 cut(s) 431
PsuI RGATCY 2 cut(s) 60, 912
RsaI GTAC 3 cut(s) 452, 940, 953
RsaNI GTAC 3 cut(s) 451, 939, 952
RseI CAYNNNNRTG 3 cut(s) 479, 687, 747
SacII CCGCGG 2 cut(s) 82, 234
SaqAI TTAA 3 cut(s) 15, 419, 777
SatI GCNGC 7 cut(s) 82, 201, 231, 234, 247, 324, 587
Sau3AI GATC 4 cut(s) 60, 465, 691, 912
Sau96I GGNCC 4 cut(s) 424, 425, 548, 903
SchI GAGTC 3 cut(s) 197, 348, 830
ScrFI CCNGG 4 cut(s) 86, 272, 488, 862
SduI GDGCHC 3 cut(s) 95, 428, 438
SfaNI GCATC 4 cut(s) 10, 258, 483, 691
SfiI GGCCNNNNNGGCC 1 cut(s) 81
Sfr274I CTCGAG 1 cut(s) 431
Sfr303I CCGCGG 2 cut(s) 82, 234
SgrBI CCGCGG 2 cut(s) 82, 234
SinI GGWCC 1 cut(s) 903
SlaI CTCGAG 1 cut(s) 431
SmiMI CAYNNNNRTG 3 cut(s) 479, 687, 747
SmlI CTYRAG 3 cut(s) 431, 667, 920
SmoI CTYRAG 3 cut(s) 431, 667, 920
SphI GCATGC 1 cut(s) 532
Sse9I AATT 3 cut(s) 215, 574, 791
SspMI CTAG 1 cut(s) 122
StyD4I CCNGG 4 cut(s) 84, 270, 486, 860
StyI CCWWGG 2 cut(s) 121, 618
TaaI ACNGT 1 cut(s) 731
TaiI ACGT 4 cut(s) 186, 315, 585, 687
TaqI TCGA 4 cut(s) 162, 186, 318, 432
TasI AATT 3 cut(s) 215, 574, 791
TauI GCSGC 6 cut(s) 84, 203, 233, 236, 249, 326
TfiI GAWTC 3 cut(s) 333, 407, 499
Tru1I TTAA 3 cut(s) 15, 419, 777
Tru9I TTAA 3 cut(s) 15, 419, 777
TscAI CASTG 1 cut(s) 736
TseFI GTSAC 2 cut(s) 340, 769
TseI GCWGC 1 cut(s) 586
Tsp45I GTSAC 2 cut(s) 340, 769
TspDTI ATGAA 1 cut(s) 271
TspRI CASTG 1 cut(s) 736
Van91I CCANNNNNTGG 1 cut(s) 447
VpaK11BI GGWCC 1 cut(s) 903
XapI RAATTY 1 cut(s) 574
XceI RCATGY 2 cut(s) 532, 746
XcmI CCANNNNNNNNNTGG 2 cut(s) 640, 868
XhoI CTCGAG 1 cut(s) 431
XmaJI CCTAGG 1 cut(s) 121
XspI CTAG 1 cut(s) 122
ZraI GACGTC 1 cut(s) 184
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.