AT1G73340

cytochrome P450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0012294
Physical Location & Seq
Forward (+)
27573114 .. 27575273
2160 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G73340.1

Sequence Viewer

Length: 1464 bp
ATGGCGGAATCTGCAGGAGAATCCTATAGGCTTCTCTCTGTTTCTTCATCAACCACATTTCTTGCCTTCATAATCATCTTCTTACTTGCGGGTATAGCAAGAAGAAAGAGAAGAGCGCCTCACAGGCTGCCTCCGGGAAGCAGAGGATGGCCTTTGATCGGAGATACCTTCGCTTGGCTCAACGCCGTCGCTGGTTCTCATCCTTCAAGCTTCGTCGAGAAACAAATAAAAAAGTATGGGAGGATATTCTCGTGTAGCTTGTTTGGGAAATGGGCAGTGGTATCAGCAGACCCGGATTTTAACCGGTTTATAATGCAGAACGAAGGAAAGCTATTTCAGTCAAGTTATCCAAAGTCTTTCAGGGATTTGGTTGGGAAAGATGGAGTGATCACAGTGCATGGTGATCAGCAGAGACGGCTTCACTCGATTGCATCGAGCATGATGCGTCATGACCAGCTCAAGACTCATTTTCTTGAGGTTATTCCCGTCGTTATGCTCCAGACACTGAGTAATTTCAAGGACGGTGAAGTCGTGCTTCTCCAAGATATCTGCAGAAAGGTTGCCATTCATCTTATGGTTAACCAACTTCTCGGTGTGTCGAGCGAATCAGAAGTCGATGAAATGTCTCAGCTTTTCTCTGATTTTGTTGATGGATGTCTCTCTGTTCCTATTGACTTGCCTGGTTTCACATACAACAAAGCAATGAAGGCACGAAAGGAGATCATAAGGAAGATAAACAAGACGATAGAAAAGCGGTTGCAAAACAAAGCAGCATCAGATACTGCTGGTAATGGTGTACTTGGAAGATTGCTTGAAGAAGAAAGTTTACCAAACGAATCAATGGCAGATTTTATCATCAATCTACTGTTTGCCGGAAATGAAACAACCTCAAAAACAATGCTGTTTGCGGTTTACTTCCTCACTCATTGTCCTAAGGCCATGACCCAACTCCTGGAGGAACATGATAGGCTCGCGGGTGGAATGTTAACATGGCAAGATTACAAGACAATGGACTTCACTCAATGCGTAATAGATGAAACACTCAGGCTTGGTGGAATTGCGATATGGCTTATGAGAGAGGCCAAAGAAGATGTCTCATATCAAGATTATGTCATTCCCAAGGGATGCTTCGTAGTTCCGTTTCTCTCGGCAGTGCACTTAGACGAGAGCTATTACAAAGAAAGTCTCTCCTTCAATCCATGGCGATGGCTTGACCCTGAAACTCAGCAAAAGAGGAATTGGAGAACGAGCCCTTTTTATTGCCCATTTGGTGGAGGAACTCGGTTTTGCCCAGGAGCAGAACTGGCTCGCCTTCAAATTGCTCTCTTTCTGCATTACTTCATCACCACATATAAGTGGACACAACTTAAGGAAGATCGTATCTCCTTCTTCCCTTCGGCTCGTCTAGTGAATGGCTTCAAAATCCAGTTGAACAGACGAGACAGTGATCCTCCGAATCAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
p450

Protein Analysis

487

Amino Acids

55.47

Weight (kDa)

8.75

Isoelectric Point (pI)

41.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 44 - 460 6.8e-61 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012294)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G73340
fragaria_vesca FvH4_5g18610
malus_domestica MD14G1247400.v1.1
prunus_persica Prupe.5G245500_v2.0.a1
pyrus_communis pycom2874g00050
rosa_chinensis RchiOBHm_Chr7g0176891
rosa_laevigata RLG00000005575
rosa_multiflora Rmu_sc0005310.1_g000048
rosa_roxburghii Rroxscaffold_3G00276310
rosa_rugosa Rorug06G0401400 Rorug06G0401500 Rorug06G0401500 Rorug06G0401500 Rorug06G0401600
rosa_samantha Rh7AG001100 Rh7BG001500 Rh7CG000900 Rh7DG001200
rosa_wichuraiana Rw7G000080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 311
AasI GACNNNNNNGTC 1 cut(s) 527
AccB7I CCANNNNNTGG 2 cut(s) 952, 1271
AccII CGCG 1 cut(s) 974
AciI CCGC 5 cut(s) 5, 89, 754, 908, 974
AclWI GGATC 1 cut(s) 1442
AfaI GTAC 1 cut(s) 798
AfiI CCNNNNNNNGG 4 cut(s) 158, 174, 952, 1271
AflII CTTAAG 1 cut(s) 1367
AgeI ACCGGT 1 cut(s) 303
AgsI TTSAA 7 cut(s) 207, 517, 815, 1195, 1316, 1420, 1432
AjnI CCWGG 3 cut(s) 679, 951, 1291
AjuI GAANNNNNNNTTGG 2 cut(s) 1112, 1144
AluBI AGCT 6 cut(s) 210, 258, 331, 457, 631, 1170
AluI AGCT 6 cut(s) 210, 258, 331, 457, 631, 1170
Alw21I GWGCWC 1 cut(s) 1158
Alw26I GTCTC 6 cut(s) 406, 630, 662, 1099, 1190, 1434
Alw44I GTGCAC 1 cut(s) 1154
AlwI GGATC 1 cut(s) 1442
AlwNI CAGNNNCTG 2 cut(s) 505, 782
AoxI GGCC 3 cut(s) 149, 936, 1080
ApaLI GTGCAC 1 cut(s) 1154
ApeKI GCWGC 2 cut(s) 127, 770
ArsI GACNNNNNNTTYG 4 cut(s) 597, 629, 1077, 1109
AsiGI ACCGGT 1 cut(s) 303
Asp700I GAANNNNTTC 1 cut(s) 1415
AspLEI GCGC 1 cut(s) 118
AsuC2I CCSGG 2 cut(s) 135, 293
AsuHPI GGTGA 3 cut(s) 413, 536, 1336
AxyI CCTNAGG 1 cut(s) 933
BaeGI GKGCMC 1 cut(s) 1158
BanII GRGCYC 1 cut(s) 1253
BarI GAAGNNNNNNTAC 2 cut(s) 810, 842
BauI CACGAG 1 cut(s) 250
Bbv12I GWGCWC 1 cut(s) 1158
BbvI GCAGC 2 cut(s) 114, 782
BccI CCATC 4 cut(s) 141, 374, 644, 1200
BceAI ACGGC 2 cut(s) 170, 431
BcgI CGANNNNNNTGC 2 cut(s) 424, 458
BciT130I CCWGG 3 cut(s) 681, 953, 1293
BclI TGATCA 2 cut(s) 387, 403
BcnI CCSGG 2 cut(s) 135, 293
BcoDI GTCTC 6 cut(s) 406, 630, 662, 1099, 1190, 1434
BfaI CTAG 1 cut(s) 1406
BfmI CTRYAG 3 cut(s) 12, 25, 550
BfoI RGCGCY 1 cut(s) 119
BfrI CTTAAG 1 cut(s) 1367
BglI GCCNNNNNGGC 1 cut(s) 124
BisI GCNGC 2 cut(s) 128, 771
BlsI GCNGC 2 cut(s) 129, 772
Bme1390I CCNGG 5 cut(s) 135, 293, 681, 953, 1293
BmrFI CCNGG 5 cut(s) 135, 293, 681, 953, 1293
BmsI GCATC 4 cut(s) 432, 440, 782, 1115
BpmI CTGGAG 2 cut(s) 482, 974
BpuEI CTTGAG 2 cut(s) 443, 494
BpuMI CCSGG 2 cut(s) 135, 293
BsaJI CCNNGG 3 cut(s) 1119, 1199, 1291
BsaWI WCCGGW 1 cut(s) 303
Bsc4I CCNNNNNNNGG 4 cut(s) 158, 174, 952, 1271
Bse118I RCCGGY 1 cut(s) 303
Bse1I ACTGG 2 cut(s) 1308, 1426
Bse21I CCTNAGG 1 cut(s) 933
Bse3DI GCAATG 1 cut(s) 708
BseBI CCWGG 3 cut(s) 681, 953, 1293
BseDI CCNNGG 3 cut(s) 1119, 1199, 1291
BseGI GGATG 4 cut(s) 152, 199, 659, 1130
BseLI CCNNNNNNNGG 4 cut(s) 158, 174, 952, 1271
BseMI GCAATG 1 cut(s) 708
BseMII CTCAG 4 cut(s) 497, 641, 1057, 1238
BseNI ACTGG 2 cut(s) 1308, 1426
BseSI GKGCMC 1 cut(s) 1158
BseXI GCAGC 2 cut(s) 114, 782
Bsh1236I CGCG 1 cut(s) 974
BshFI GGCC 3 cut(s) 151, 938, 1082
BshTI ACCGGT 1 cut(s) 303
BsiHKAI GWGCWC 1 cut(s) 1158
BsiSI CCGG 4 cut(s) 134, 293, 304, 873
BslI CCNNNNNNNGG 4 cut(s) 158, 174, 952, 1271
BsmAI GTCTC 6 cut(s) 406, 630, 662, 1099, 1190, 1434
BsmBI CGTCTC 1 cut(s) 406
BsnI GGCC 3 cut(s) 151, 938, 1082
Bsp1286I GDGCHC 2 cut(s) 1158, 1253
Bsp143I GATC 6 cut(s) 156, 387, 403, 720, 1375, 1447
Bsp19I CCATGG 1 cut(s) 1199
BspACI CCGC 5 cut(s) 5, 89, 754, 908, 974
BspANI GGCC 3 cut(s) 151, 938, 1082
BspCNI CTCAG 4 cut(s) 498, 640, 1056, 1237
BspFNI CGCG 1 cut(s) 974
BspHI TCATGA 1 cut(s) 448
BspMAI CTGCAG 2 cut(s) 16, 554
BspPI GGATC 1 cut(s) 1442
BspQI GCTCTTC 1 cut(s) 106
BspTI CTTAAG 1 cut(s) 1367
BsrDI GCAATG 1 cut(s) 708
BsrFI RCCGGY 1 cut(s) 303
BsrI ACTGG 2 cut(s) 1308, 1426
BssAI RCCGGY 1 cut(s) 303
BssECI CCNNGG 3 cut(s) 1119, 1199, 1291
BssMI GATC 6 cut(s) 156, 387, 403, 720, 1375, 1447
BssSI CACGAG 1 cut(s) 250
BssT1I CCWWGG 2 cut(s) 1119, 1199
Bst2BI CACGAG 1 cut(s) 250
Bst2UI CCWGG 3 cut(s) 681, 953, 1293
Bst4CI ACNGT 4 cut(s) 394, 524, 867, 1445
Bst6I CTCTTC 1 cut(s) 106
BstAFI CTTAAG 1 cut(s) 1367
BstC8I GCNNGC 2 cut(s) 972, 1309
BstDEI CTNAG 6 cut(s) 506, 627, 933, 1043, 1159, 1224
BstDSI CCRYGG 1 cut(s) 1199
BstF5I GGATG 4 cut(s) 152, 199, 659, 1130
BstFNI CGCG 1 cut(s) 974
BstH2I RGCGCY 1 cut(s) 119
BstHHI GCGC 1 cut(s) 118
BstKTI GATC 6 cut(s) 159, 390, 406, 723, 1378, 1450
BstMAI GTCTC 6 cut(s) 406, 630, 662, 1099, 1190, 1434
BstMBI GATC 6 cut(s) 156, 387, 403, 720, 1375, 1447
BstMWI GCNNNNNNNGC 6 cut(s) 11, 95, 124, 415, 707, 1304
BstNI CCWGG 3 cut(s) 681, 953, 1293
BstSCI CCNGG 5 cut(s) 133, 291, 679, 951, 1291
BstSFI CTRYAG 3 cut(s) 12, 25, 550
BstSLI GKGCMC 1 cut(s) 1158
BstUI CGCG 1 cut(s) 974
BstV1I GCAGC 2 cut(s) 114, 782
BstXI CCANNNNNNTGG 1 cut(s) 1206
Bsu36I CCTNAGG 1 cut(s) 933
BsuRI GGCC 3 cut(s) 151, 938, 1082
BtgI CCRYGG 1 cut(s) 1199
BtgZI GCGATG 1 cut(s) 1219
BtsCI GGATG 4 cut(s) 152, 199, 659, 1130
BtsI GCAGTG 2 cut(s) 282, 1158
BtsIMutI CAGTG 5 cut(s) 282, 399, 503, 1158, 1450
Cac8I GCNNGC 2 cut(s) 972, 1309
CaiI CAGNNNCTG 2 cut(s) 505, 782
CciI TCATGA 1 cut(s) 448
CfoI GCGC 1 cut(s) 118
Cfr10I RCCGGY 1 cut(s) 303
CseI GACGC 1 cut(s) 434
Csp6I GTAC 1 cut(s) 797
CspAI ACCGGT 1 cut(s) 303
CspCI CAANNNNNGTGG 2 cut(s) 43, 78
CviAII CATG 7 cut(s) 398, 439, 449, 940, 962, 990, 1200
CviQI GTAC 1 cut(s) 797
DdeI CTNAG 6 cut(s) 506, 627, 933, 1043, 1159, 1224
DpnI GATC 6 cut(s) 158, 389, 405, 722, 1377, 1449
DpnII GATC 6 cut(s) 156, 387, 403, 720, 1375, 1447
DrdI GACNNNNNNGTC 1 cut(s) 527
DseDI GACNNNNNNGTC 1 cut(s) 527
Eam1104I CTCTTC 1 cut(s) 106
EarI CTCTTC 1 cut(s) 106
EciI GGCGGA 1 cut(s) 20
Eco130I CCWWGG 2 cut(s) 1119, 1199
Eco24I GRGCYC 1 cut(s) 1253
Eco32I GATATC 1 cut(s) 547
Eco81I CCTNAGG 1 cut(s) 933
EcoRII CCWGG 3 cut(s) 679, 951, 1291
EcoRV GATATC 1 cut(s) 547
EcoT14I CCWWGG 2 cut(s) 1119, 1199
EcoT38I GRGCYC 1 cut(s) 1253
ErhI CCWWGG 2 cut(s) 1119, 1199
Esp3I CGTCTC 1 cut(s) 406
FaeI CATG 7 cut(s) 401, 442, 452, 943, 965, 993, 1203
FalI AAGNNNNNCTT 4 cut(s) 519, 551, 1112, 1144
FatI CATG 7 cut(s) 397, 438, 448, 939, 961, 989, 1199
FauI CCCGC 2 cut(s) 82, 967
FbaI TGATCA 2 cut(s) 387, 403
Fnu4HI GCNGC 2 cut(s) 128, 771
FokI GGATG 4 cut(s) 159, 186, 666, 1137
FriOI GRGCYC 1 cut(s) 1253
Fsp4HI GCNGC 2 cut(s) 128, 771
FspBI CTAG 1 cut(s) 1406
GlaI GCGC 1 cut(s) 117
GluI GCNGC 2 cut(s) 128, 771
GsuI CTGGAG 2 cut(s) 482, 974
HaeII RGCGCY 1 cut(s) 119
HaeIII GGCC 3 cut(s) 151, 938, 1082
HapII CCGG 4 cut(s) 134, 293, 304, 873
HgaI GACGC 1 cut(s) 434
HhaI GCGC 1 cut(s) 118
Hin1II CATG 7 cut(s) 401, 442, 452, 943, 965, 993, 1203
Hin6I GCGC 1 cut(s) 116
HinP1I GCGC 1 cut(s) 116
HincII GTYRAC 2 cut(s) 580, 987
HindII GTYRAC 2 cut(s) 580, 987
HindIII AAGCTT 1 cut(s) 208
HinfI GANTC 6 cut(s) 8, 20, 463, 605, 836, 1456
HpaI GTTAAC 2 cut(s) 580, 987
HpaII CCGG 4 cut(s) 134, 293, 304, 873
HphI GGTGA 3 cut(s) 413, 536, 1336
Hpy166II GTNNAC 7 cut(s) 580, 797, 827, 913, 987, 1156, 1359
Hpy188I TCNGA 5 cut(s) 161, 610, 640, 778, 1455
Hpy188III TCNNGA 6 cut(s) 217, 449, 460, 473, 499, 1103
Hpy8I GTNNAC 7 cut(s) 580, 797, 827, 913, 987, 1156, 1359
Hpy99I CGWCG 3 cut(s) 191, 218, 491
HpyAV CCTTC 9 cut(s) 76, 178, 213, 317, 700, 1201, 1322, 1396, 1404
HpyCH4III ACNGT 4 cut(s) 394, 524, 867, 1445
HpyCH4V TGCA 8 cut(s) 14, 316, 397, 431, 552, 760, 1156, 1333
HpyF10VI GCNNNNNNNGC 6 cut(s) 11, 95, 124, 415, 707, 1304
HpyF3I CTNAG 6 cut(s) 506, 627, 933, 1043, 1159, 1224
Hsp92II CATG 7 cut(s) 401, 442, 452, 943, 965, 993, 1203
HspAI GCGC 1 cut(s) 116
Ksp22I TGATCA 2 cut(s) 387, 403
KspAI GTTAAC 2 cut(s) 580, 987
Kzo9I GATC 6 cut(s) 156, 387, 403, 720, 1375, 1447
LguI GCTCTTC 1 cut(s) 106
LmnI GCTCC 2 cut(s) 501, 1295
Lsp1109I GCAGC 2 cut(s) 114, 782
LweI GCATC 4 cut(s) 432, 440, 782, 1115
MaeI CTAG 1 cut(s) 1406
MalI GATC 6 cut(s) 158, 389, 405, 722, 1377, 1449
MboI GATC 6 cut(s) 156, 387, 403, 720, 1375, 1447
MhlI GDGCHC 2 cut(s) 1158, 1253
MluCI AATT 4 cut(s) 511, 1056, 1237, 1317
MlyI GAGTC 1 cut(s) 457
MroXI GAANNNNTTC 1 cut(s) 1415
MseI TTAA 4 cut(s) 300, 579, 986, 1368
MslI CAYNNNNRTG 2 cut(s) 1204, 1354
MspCI CTTAAG 1 cut(s) 1367
MspI CCGG 4 cut(s) 134, 293, 304, 873
MspR9I CCNGG 5 cut(s) 135, 293, 681, 953, 1293
MvaI CCWGG 3 cut(s) 681, 953, 1293
MvnI CGCG 1 cut(s) 974
MwoI GCNNNNNNNGC 6 cut(s) 11, 95, 124, 415, 707, 1304
NciI CCSGG 2 cut(s) 135, 293
NcoI CCATGG 1 cut(s) 1199
NdeII GATC 6 cut(s) 156, 387, 403, 720, 1375, 1447
NlaIII CATG 7 cut(s) 401, 442, 452, 943, 965, 993, 1203
NmeAIII GCCGAG 1 cut(s) 1127
PagI TCATGA 1 cut(s) 448
PciSI GCTCTTC 1 cut(s) 106
PcsI WCGNNNNNNNCGW 2 cut(s) 431, 528
PdmI GAANNNNTTC 1 cut(s) 1415
PfeI GAWTC 5 cut(s) 8, 20, 605, 836, 1456
PflMI CCANNNNNTGG 2 cut(s) 952, 1271
PfoI TCCNGGA 2 cut(s) 133, 951
PinAI ACCGGT 1 cut(s) 303
PkrI GCNGC 2 cut(s) 129, 772
PleI GAGTC 1 cut(s) 457
PpsI GAGTC 1 cut(s) 457
PsiI TTATAA 1 cut(s) 311
Psp6I CCWGG 3 cut(s) 679, 951, 1291
PspGI CCWGG 3 cut(s) 679, 951, 1291
PstI CTGCAG 2 cut(s) 16, 554
PstNI CAGNNNCTG 2 cut(s) 505, 782
RsaI GTAC 1 cut(s) 798
RsaNI GTAC 1 cut(s) 797
RseI CAYNNNNRTG 2 cut(s) 1204, 1354
SapI GCTCTTC 1 cut(s) 106
SaqAI TTAA 4 cut(s) 300, 579, 986, 1368
SatI GCNGC 2 cut(s) 128, 771
Sau3AI GATC 6 cut(s) 156, 387, 403, 720, 1375, 1447
SchI GAGTC 1 cut(s) 457
ScrFI CCNGG 5 cut(s) 135, 293, 681, 953, 1293
SduI GDGCHC 2 cut(s) 1158, 1253
SfaNI GCATC 4 cut(s) 432, 440, 782, 1115
SfcI CTRYAG 3 cut(s) 12, 25, 550
SmiMI CAYNNNNRTG 2 cut(s) 1204, 1354
SmlI CTYRAG 3 cut(s) 458, 473, 1367
SmoI CTYRAG 3 cut(s) 458, 473, 1367
Sse9I AATT 4 cut(s) 511, 1056, 1237, 1317
SsiI CCGC 5 cut(s) 5, 89, 754, 908, 974
SspMI CTAG 1 cut(s) 1406
StyD4I CCNGG 5 cut(s) 133, 291, 679, 951, 1291
StyI CCWWGG 2 cut(s) 1119, 1199
TaaI ACNGT 4 cut(s) 394, 524, 867, 1445
TaqI TCGA 5 cut(s) 216, 425, 434, 599, 615
TasI AATT 4 cut(s) 511, 1056, 1237, 1317
TatI WGTACW 1 cut(s) 796
TfiI GAWTC 5 cut(s) 8, 20, 605, 836, 1456
Tru1I TTAA 4 cut(s) 300, 579, 986, 1368
Tru9I TTAA 4 cut(s) 300, 579, 986, 1368
TscAI CASTG 5 cut(s) 282, 399, 510, 1158, 1450
TseI GCWGC 2 cut(s) 127, 770
TspDTI ATGAA 8 cut(s) 36, 58, 557, 633, 719, 894, 1050, 1330
TspGWI ACGGA 1 cut(s) 1128
TspRI CASTG 5 cut(s) 282, 399, 510, 1158, 1450
Van91I CCANNNNNTGG 2 cut(s) 952, 1271
Vha464I CTTAAG 1 cut(s) 1367
VneI GTGCAC 1 cut(s) 1154
XcmI CCANNNNNNNNNTGG 1 cut(s) 571
XmnI GAANNNNTTC 1 cut(s) 1415
XspI CTAG 1 cut(s) 1406
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.