MD14G1247400.v1.1

Belongs to the cytochrome P450 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0012294
Physical Location & Seq
Forward (+)
32480897 .. 32487922
7026 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1247400.v1.1.491

Sequence Viewer

Length: 1446 bp
ATGGGAAACCTTTCGACCTGCCTGCATTTGATCTCCACAGTATTGTTCATTTCTTTTGTTGCAAAGTTGTTGGTGGTAAGGATCAGAAAAAGAAAGCTCAAGATTGGCCAATTGCCACCAGGAAGAAGAGGTTGGCCAATCGTTGGCGACAGCTTCAATTGGTACAATGCGGTCGCGAGTTCCCACCCCCCTCAGTTTGTTGAACAACAAGTCCAGAAGTTCGGGAAGATATTCTCATGCAGTCTGTTTGGAAGATGGTCTGTGATATCAGCAGATCCAATCTTCAACCGCTTTGTAATGCAGAATGAAGGGAAACTGTTCCAGTCTAGTTATCCAAAATCTTTTAAAGATTTGGTTGGCAAAAATGGTGTTATCACGGTGCACGGAGAGCCACAACGAAAGCTCCACGGAATCGCCTCCAACATGATGCGCCTAGACCACAAACTTAAATCGAGGTTTAATTTCTTGGACGACATTCAAATGATTATGACTCAAACTTTGAGCAATTTTCAAACCAATCAATCGATACTTCTCCAGGATGTTTGCAGAAAGGTGGCTATAAATCTAATGGTTAATCAACTTTTGGGAGTGTCGAGCGATTCAGAGATTAATGAGATGGCTCAACTGTTCTCCGACTTCGTTGATGGTTGTCTCTCTTTTCCAATCAACTTGCCTGGTTTTGCATACCACACTGCCATGAAGGCAAGGGAAAAAATCATAATCAAGATAAATAGGATAACGCAAAATCACAGAGAACAACGTGAAGTTGGCAATGGCGTCCTTGGAAGACTGTTGGAGGAAGAAAGCTTACGGGATGAAGCTGTACCAGACTTCATTATCAACCTTCTCTTTGCTGGGAATGAAACAACTGCTAAAACAATGCTATTTGCAGTCTATTTCCTTACTCAGTGTCCCAGAGCAATGAAACAATTAGTGGATGAACAAGACAGCTTCCGAAGCAAACCTGTCGGAGAAGAAACTCTTACATGGCAGGATTACAAAGCAATGTCTTTTACTCAATGTGTCATTGATGAAACACTTCGACTCGGAGGAATTGCAATTTGGTTAATGAGGGAGGCAAAAGAAGACGTTGTGTACCAAGACTATGTTATCCCCAAAGGATGCTTTGTTGTTCCCTTCCTTTCAGCAGTTCATTTGGATGAAAATGTGTACAGAGAATCCCTCACTTTTAACCCATGGAGATGGATGGATCTTGAAAATCAGGAGAAAAGAAATTGGAGAACTAGCCCATTTTTTGCACCCTTTGGAGGAGGAGCCAGATTCTGTCCGGGGGCTGAGTTGGCTCGCCTTCAAATTACCCTTTTTCTCCATTACTTTGTTACAACATATAGATGGACCCAACTAAAAGAAGACCGGATGTCATTCTTTCCCTCAGCTCGTTTAGTGAATGGCTTCCAGATCTGCTTAACTAGACGGTCGACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
p450

Protein Analysis

482

Amino Acids

55.34

Weight (kDa)

9.18

Isoelectric Point (pI)

35.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 39 - 461 1.4e-58 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012294)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G73340
fragaria_vesca FvH4_5g18610
malus_domestica MD14G1247400.v1.1
prunus_persica Prupe.5G245500_v2.0.a1
pyrus_communis pycom2874g00050
rosa_chinensis RchiOBHm_Chr7g0176891
rosa_laevigata RLG00000005575
rosa_multiflora Rmu_sc0005310.1_g000048
rosa_roxburghii Rroxscaffold_3G00276310
rosa_rugosa Rorug06G0401400 Rorug06G0401500 Rorug06G0401500 Rorug06G0401500 Rorug06G0401600
rosa_samantha Rh7AG001100 Rh7BG001500 Rh7CG000900 Rh7DG001200
rosa_wichuraiana Rw7G000080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 26
AccB7I CCANNNNNTGG 1 cut(s) 143
AccI GTMKAC 1 cut(s) 1439
AccII CGCG 1 cut(s) 176
AciI CCGC 2 cut(s) 170, 289
AclWI GGATC 3 cut(s) 89, 269, 1218
AcoI YGGCCR 2 cut(s) 106, 134
AcyI GRCGYC 1 cut(s) 777
AfaI GTAC 4 cut(s) 164, 825, 1097, 1172
AfiI CCNNNNNNNGG 2 cut(s) 143, 1268
AgsI TTSAA 7 cut(s) 157, 203, 286, 479, 512, 1217, 1313
AhdI GACNNNNNGTC 1 cut(s) 1378
AjnI CCWGG 3 cut(s) 118, 534, 673
AjuI GAANNNNNNNTTGG 4 cut(s) 115, 147, 1045, 1077
AloI GAACNNNNNNTCC 2 cut(s) 195, 227
AluBI AGCT 7 cut(s) 97, 153, 403, 807, 821, 951, 1397
AluI AGCT 7 cut(s) 97, 153, 403, 807, 821, 951, 1397
Alw21I GWGCWC 1 cut(s) 384
Alw26I GTCTC 1 cut(s) 656
Alw44I GTGCAC 1 cut(s) 380
AlwI GGATC 3 cut(s) 89, 269, 1218
AlwNI CAGNNNCTG 1 cut(s) 1284
AoxI GGCC 2 cut(s) 106, 134
ApaLI GTGCAC 1 cut(s) 380
ArsI GACNNNNNNTTYG 2 cut(s) 481, 513
AseI ATTAAT 1 cut(s) 609
Asp700I GAANNNNTTC 5 cut(s) 10, 230, 317, 1038, 1412
AspLEI GCGC 1 cut(s) 432
AspS9I GGNCC 1 cut(s) 1356
AsuC2I CCSGG 1 cut(s) 1290
AvaII GGWCC 1 cut(s) 1356
BaeGI GKGCMC 1 cut(s) 384
BalI TGGCCA 2 cut(s) 108, 136
BarI GAAGNNNNNNTAC 2 cut(s) 792, 824
BbsI GAAGAC 3 cut(s) 793, 1092, 1377
Bbv12I GWGCWC 1 cut(s) 384
BbvCI CCTCAGC 1 cut(s) 1393
BccI CCATC 6 cut(s) 249, 610, 638, 1197, 1201, 1347
BcgI CGANNNNNNTGC 4 cut(s) 4, 38, 949, 983
BciT130I CCWGG 3 cut(s) 120, 536, 675
BcnI CCSGG 1 cut(s) 1290
BcoDI GTCTC 1 cut(s) 656
BfaI CTAG 4 cut(s) 327, 434, 1245, 1431
BfuAI ACCTGC 1 cut(s) 26
BglI GCCNNNNNGGC 1 cut(s) 701
BglII AGATCT 1 cut(s) 1419
Bme1390I CCNGG 4 cut(s) 120, 536, 675, 1290
Bme18I GGWCC 1 cut(s) 1356
BmeRI GACNNNNNGTC 1 cut(s) 1378
BmgT120I GGNCC 1 cut(s) 1356
BmiI GGNNCC 2 cut(s) 1276, 1358
BmrFI CCNGG 4 cut(s) 120, 536, 675, 1290
BmsI GCATC 2 cut(s) 417, 1112
BpiI GAAGAC 3 cut(s) 793, 1092, 1377
BplI GAGNNNNNCTC 2 cut(s) 1167, 1199
BpmI CTGGAG 1 cut(s) 518
Bpu10I CCTNAGC 1 cut(s) 1393
BpuEI CTTGAG 1 cut(s) 83
BpuMI CCSGG 1 cut(s) 1290
Bsa29I ATCGAT 1 cut(s) 524
BsaHI GRCGYC 1 cut(s) 777
BsaJI CCNNGG 4 cut(s) 406, 781, 1196, 1289
BsaWI WCCGGW 1 cut(s) 1374
BsaXI ACNNNNNCTCC 2 cut(s) 387, 417
Bsc4I CCNNNNNNNGG 2 cut(s) 143, 1268
Bse1I ACTGG 1 cut(s) 322
Bse3DI GCAATG 3 cut(s) 778, 927, 1011
BseBI CCWGG 3 cut(s) 120, 536, 675
BseCI ATCGAT 1 cut(s) 524
BseDI CCNNGG 4 cut(s) 406, 781, 1196, 1289
BseGI GGATG 7 cut(s) 544, 820, 943, 1127, 1165, 1212, 1383
BseLI CCNNNNNNNGG 2 cut(s) 143, 1268
BseMI GCAATG 3 cut(s) 778, 927, 1011
BseMII CTCAG 4 cut(s) 206, 920, 1287, 1407
BseNI ACTGG 1 cut(s) 322
BseRI GAGGAG 2 cut(s) 1284, 1287
BseSI GKGCMC 1 cut(s) 384
BseYI CCCAGC 1 cut(s) 854
Bsh1236I CGCG 1 cut(s) 176
Bsh1285I CGRYCG 2 cut(s) 174, 1439
BshFI GGCC 2 cut(s) 108, 136
BshVI ATCGAT 1 cut(s) 524
BsiEI CGRYCG 2 cut(s) 174, 1439
BsiHKAI GWGCWC 1 cut(s) 384
BsiSI CCGG 2 cut(s) 1289, 1375
BslFI GGGAC 1 cut(s) 897
BslI CCNNNNNNNGG 2 cut(s) 143, 1268
BsmAI GTCTC 1 cut(s) 656
BsmFI GGGAC 1 cut(s) 897
BsnI GGCC 2 cut(s) 108, 136
Bsp1286I GDGCHC 1 cut(s) 384
Bsp1407I TGTACA 1 cut(s) 1170
Bsp143I GATC 5 cut(s) 30, 81, 274, 1210, 1419
Bsp19I CCATGG 1 cut(s) 1196
Bsp68I TCGCGA 1 cut(s) 176
BspACI CCGC 2 cut(s) 170, 289
BspANI GGCC 2 cut(s) 108, 136
BspCNI CTCAG 4 cut(s) 205, 919, 1288, 1406
BspDI ATCGAT 1 cut(s) 524
BspFNI CGCG 1 cut(s) 176
BspLI GGNNCC 2 cut(s) 1276, 1358
BspMI ACCTGC 1 cut(s) 26
BspPI GGATC 3 cut(s) 89, 269, 1218
BsrDI GCAATG 3 cut(s) 778, 927, 1011
BsrGI TGTACA 1 cut(s) 1170
BsrI ACTGG 1 cut(s) 322
BssECI CCNNGG 4 cut(s) 406, 781, 1196, 1289
BssMI GATC 5 cut(s) 30, 81, 274, 1210, 1419
BssNI GRCGYC 1 cut(s) 777
BssT1I CCWWGG 2 cut(s) 781, 1196
Bst2UI CCWGG 3 cut(s) 120, 536, 675
Bst4CI ACNGT 6 cut(s) 40, 318, 379, 627, 792, 1437
Bst6I CTCTTC 1 cut(s) 121
BstACI GRCGYC 1 cut(s) 777
BstAUI TGTACA 1 cut(s) 1170
BstC8I GCNNGC 2 cut(s) 23, 1306
BstDEI CTNAG 4 cut(s) 192, 906, 1296, 1393
BstDSI CCRYGG 2 cut(s) 406, 1196
BstF5I GGATG 7 cut(s) 544, 820, 943, 1127, 1165, 1212, 1383
BstFNI CGCG 1 cut(s) 176
BstHHI GCGC 1 cut(s) 432
BstKTI GATC 5 cut(s) 33, 84, 277, 1213, 1422
BstMAI GTCTC 1 cut(s) 656
BstMBI GATC 5 cut(s) 30, 81, 274, 1210, 1419
BstMCI CGRYCG 2 cut(s) 174, 1439
BstMWI GCNNNNNNNGC 4 cut(s) 388, 701, 957, 1301
BstNI CCWGG 3 cut(s) 120, 536, 675
BstSCI CCNGG 4 cut(s) 118, 534, 673, 1288
BstSLI GKGCMC 1 cut(s) 384
BstUI CGCG 1 cut(s) 176
BstV2I GAAGAC 3 cut(s) 793, 1092, 1377
BstX2I RGATCY 3 cut(s) 274, 1210, 1419
BstXI CCANNNNNNTGG 1 cut(s) 1203
BstYI RGATCY 3 cut(s) 274, 1210, 1419
Bsu15I ATCGAT 1 cut(s) 524
BsuRI GGCC 2 cut(s) 108, 136
BsuTUI ATCGAT 1 cut(s) 524
BtgI CCRYGG 2 cut(s) 406, 1196
BtsCI GGATG 7 cut(s) 544, 820, 943, 1127, 1165, 1212, 1383
BtsI GCAGTG 1 cut(s) 690
BtsIMutI CAGTG 2 cut(s) 690, 914
BtuMI TCGCGA 1 cut(s) 176
BveI ACCTGC 1 cut(s) 26
Cac8I GCNNGC 2 cut(s) 23, 1306
CaiI CAGNNNCTG 1 cut(s) 1284
CfoI GCGC 1 cut(s) 432
Cfr13I GGNCC 1 cut(s) 1356
ClaI ATCGAT 1 cut(s) 524
CseI GACGC 1 cut(s) 766
Csp6I GTAC 4 cut(s) 163, 824, 1096, 1171
CviAII CATG 6 cut(s) 237, 424, 697, 987, 1197, 1443
CviQI GTAC 4 cut(s) 163, 824, 1096, 1171
DdeI CTNAG 4 cut(s) 192, 906, 1296, 1393
DpnI GATC 5 cut(s) 32, 83, 276, 1212, 1421
DpnII GATC 5 cut(s) 30, 81, 274, 1210, 1419
DraI TTTAAA 1 cut(s) 346
DriI GACNNNNNGTC 1 cut(s) 1378
EaeI YGGCCR 2 cut(s) 106, 134
Eam1104I CTCTTC 1 cut(s) 121
Eam1105I GACNNNNNGTC 1 cut(s) 1378
EarI CTCTTC 1 cut(s) 121
Eco130I CCWWGG 2 cut(s) 781, 1196
Eco32I GATATC 1 cut(s) 267
Eco47I GGWCC 1 cut(s) 1356
EcoRII CCWGG 3 cut(s) 118, 534, 673
EcoRV GATATC 1 cut(s) 267
EcoT14I CCWWGG 2 cut(s) 781, 1196
ErhI CCWWGG 2 cut(s) 781, 1196
FaeI CATG 6 cut(s) 240, 427, 700, 990, 1200, 1446
FalI AAGNNNNNCTT 2 cut(s) 966, 998
FaqI GGGAC 1 cut(s) 897
FatI CATG 6 cut(s) 236, 423, 696, 986, 1196, 1442
FblI GTMKAC 1 cut(s) 1439
FokI GGATG 7 cut(s) 551, 827, 950, 1134, 1172, 1219, 1390
FspBI CTAG 4 cut(s) 327, 434, 1245, 1431
GlaI GCGC 1 cut(s) 431
GsaI CCCAGC 1 cut(s) 858
GsuI CTGGAG 1 cut(s) 518
HaeIII GGCC 2 cut(s) 108, 136
HapII CCGG 2 cut(s) 1289, 1375
HgaI GACGC 1 cut(s) 766
HhaI GCGC 1 cut(s) 432
Hin1I GRCGYC 1 cut(s) 777
Hin1II CATG 6 cut(s) 240, 427, 700, 990, 1200, 1446
Hin6I GCGC 1 cut(s) 430
HinP1I GCGC 1 cut(s) 430
HincII GTYRAC 1 cut(s) 1440
HindII GTYRAC 1 cut(s) 1440
HindIII AAGCTT 1 cut(s) 805
HinfI GANTC 6 cut(s) 411, 490, 599, 1044, 1178, 1281
HpaII CCGG 2 cut(s) 1289, 1375
Hpy166II GTNNAC 4 cut(s) 382, 1096, 1171, 1440
Hpy188I TCNGA 6 cut(s) 86, 604, 634, 956, 971, 1049
Hpy188III TCNNGA 8 cut(s) 100, 175, 214, 223, 724, 1214, 1223, 1417
Hpy8I GTNNAC 4 cut(s) 382, 1096, 1171, 1440
HpyAV CCTTC 5 cut(s) 302, 694, 854, 1147, 1319
HpyCH4III ACNGT 6 cut(s) 40, 318, 379, 627, 792, 1437
HpyCH4IV ACGT 2 cut(s) 760, 1089
HpyF10VI GCNNNNNNNGC 4 cut(s) 388, 701, 957, 1301
HpyF3I CTNAG 4 cut(s) 192, 906, 1296, 1393
HpySE526I ACGT 2 cut(s) 760, 1089
Hsp92I GRCGYC 1 cut(s) 777
Hsp92II CATG 6 cut(s) 240, 427, 700, 990, 1200, 1446
HspAI GCGC 1 cut(s) 430
Kzo9I GATC 5 cut(s) 30, 81, 274, 1210, 1419
LmnI GCTCC 2 cut(s) 408, 1274
LweI GCATC 2 cut(s) 417, 1112
MaeI CTAG 4 cut(s) 327, 434, 1245, 1431
MaeII ACGT 2 cut(s) 760, 1089
MaeIII GTNAC 1 cut(s) 1339
MalI GATC 5 cut(s) 32, 83, 276, 1212, 1421
MboI GATC 5 cut(s) 30, 81, 274, 1210, 1419
MfeI CAATTG 2 cut(s) 110, 157
MflI RGATCY 3 cut(s) 274, 1210, 1419
MhlI GDGCHC 1 cut(s) 384
MlsI TGGCCA 2 cut(s) 108, 136
MluCI AATT 9 cut(s) 110, 157, 460, 505, 929, 1053, 1059, 1234, 1314
MluNI TGGCCA 2 cut(s) 108, 136
MlyI GAGTC 2 cut(s) 484, 1038
MmeI TCCRAC 4 cut(s) 444, 657, 774, 949
Mox20I TGGCCA 2 cut(s) 108, 136
MroXI GAANNNNTTC 5 cut(s) 10, 230, 317, 1038, 1412
MscI TGGCCA 2 cut(s) 108, 136
MseI TTAA 8 cut(s) 345, 447, 459, 573, 609, 1067, 1191, 1427
MslI CAYNNNNRTG 5 cut(s) 479, 695, 1158, 1201, 1351
Msp20I TGGCCA 2 cut(s) 108, 136
MspI CCGG 2 cut(s) 1289, 1375
MspR9I CCNGG 4 cut(s) 120, 536, 675, 1290
MunI CAATTG 2 cut(s) 110, 157
MvaI CCWGG 3 cut(s) 120, 536, 675
MvnI CGCG 1 cut(s) 176
MwoI GCNNNNNNNGC 4 cut(s) 388, 701, 957, 1301
NciI CCSGG 1 cut(s) 1290
NcoI CCATGG 1 cut(s) 1196
NdeII GATC 5 cut(s) 30, 81, 274, 1210, 1419
NlaIII CATG 6 cut(s) 240, 427, 700, 990, 1200, 1446
NlaIV GGNNCC 2 cut(s) 1276, 1358
NruI TCGCGA 1 cut(s) 176
PdmI GAANNNNTTC 5 cut(s) 10, 230, 317, 1038, 1412
PfeI GAWTC 4 cut(s) 411, 599, 1178, 1281
PflMI CCANNNNNTGG 1 cut(s) 143
PfoI TCCNGGA 1 cut(s) 534
PleI GAGTC 2 cut(s) 484, 1038
PpsI GAGTC 2 cut(s) 484, 1038
PshBI ATTAAT 1 cut(s) 609
Psp6I CCWGG 3 cut(s) 118, 534, 673
PspFI CCCAGC 1 cut(s) 854
PspGI CCWGG 3 cut(s) 118, 534, 673
PspN4I GGNNCC 2 cut(s) 1276, 1358
PspPI GGNCC 1 cut(s) 1356
PstNI CAGNNNCTG 1 cut(s) 1284
PsuI RGATCY 3 cut(s) 274, 1210, 1419
RruI TCGCGA 1 cut(s) 176
RsaI GTAC 4 cut(s) 164, 825, 1097, 1172
RsaNI GTAC 4 cut(s) 163, 824, 1096, 1171
RseI CAYNNNNRTG 5 cut(s) 479, 695, 1158, 1201, 1351
SalI GTCGAC 1 cut(s) 1438
SaqAI TTAA 8 cut(s) 345, 447, 459, 573, 609, 1067, 1191, 1427
Sau3AI GATC 5 cut(s) 30, 81, 274, 1210, 1419
Sau96I GGNCC 1 cut(s) 1356
SchI GAGTC 2 cut(s) 484, 1038
ScrFI CCNGG 4 cut(s) 120, 536, 675, 1290
SduI GDGCHC 1 cut(s) 384
SfaNI GCATC 2 cut(s) 417, 1112
SinI GGWCC 1 cut(s) 1356
SmiMI CAYNNNNRTG 5 cut(s) 479, 695, 1158, 1201, 1351
SmlI CTYRAG 1 cut(s) 98
SmoI CTYRAG 1 cut(s) 98
Sse9I AATT 9 cut(s) 110, 157, 460, 505, 929, 1053, 1059, 1234, 1314
SsiI CCGC 2 cut(s) 170, 289
SspMI CTAG 4 cut(s) 327, 434, 1245, 1431
StyD4I CCNGG 4 cut(s) 118, 534, 673, 1288
StyI CCWWGG 2 cut(s) 781, 1196
TaaI ACNGT 6 cut(s) 40, 318, 379, 627, 792, 1437
TaiI ACGT 2 cut(s) 763, 1092
TaqI TCGA 6 cut(s) 14, 452, 524, 593, 1042, 1439
TasI AATT 9 cut(s) 110, 157, 460, 505, 929, 1053, 1059, 1234, 1314
TatI WGTACW 1 cut(s) 1170
TfiI GAWTC 4 cut(s) 411, 599, 1178, 1281
Tru1I TTAA 8 cut(s) 345, 447, 459, 573, 609, 1067, 1191, 1427
Tru9I TTAA 8 cut(s) 345, 447, 459, 573, 609, 1067, 1191, 1427
TscAI CASTG 2 cut(s) 697, 914
TspGWI ACGGA 2 cut(s) 399, 423
TspRI CASTG 2 cut(s) 697, 914
Van91I CCANNNNNTGG 1 cut(s) 143
VneI GTGCAC 1 cut(s) 380
VpaK11BI GGWCC 1 cut(s) 1356
VspI ATTAAT 1 cut(s) 609
XmiI GTMKAC 1 cut(s) 1439
XmnI GAANNNNTTC 5 cut(s) 10, 230, 317, 1038, 1412
XspI CTAG 4 cut(s) 327, 434, 1245, 1431
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.