AT1G73720

Transducin family protein WD-40 repeat family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0008538
Physical Location & Seq
Forward (+)
27724991 .. 27730031
5041 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G73720.1

Sequence Viewer

Length: 1536 bp
ATGGCGCTCGAAATCGAAGCTCGAGATGTAATAAAGATTATGCTTCAGTTCTGCAAAGAAAATTCCTTGAATCAGACATTCCAGACATTACAAAGCGAGTGTCAGGTTTCCTTGAATACAGTAGACAGCGTCGAGACGTTTATTTCTGATATTAATAGTGGAAGATGGGACTCGGTACTACCTCAAGTTTCCCAGCTTAAACTTCCCAGGAATAAACTTGAAGACCTTTATGAACAGATTGTATTGGAGATGATAGAACTTCGAGAGTTGGACACTGCCCGAGCTATTTTGAGACAAACTCAGGTGATGGGTGTCATGAAGCAGGAGCAAGCAGAAAGATATTTGCGGATGGAGCATCTTCTAGTCAGAAGCTATTTTGATCCGCATGAGGCTTATGGAGACTCAACTAAAGAAAGAAAACGGGCACAGATTGCTCAAGCTGTGGCTGCCGAGGTTACCGTGGTACCACCTTCACGGTTAATGGCTTTGATAGGTCAGGCTTTAAAGTGGCAACAACATCAAGGCCTGCTCCCTCCAGGAACACAGTTTGACCTCTTTCGTGGGACGGCAGCTATGAAGCAAGATGTAGAAGACACACATCCTAATGTTCTTACTCATACCATAAAGTTTGGTAAAAAGAGTCATGCAGAATGCGCTAGATTCTCACCAGATGGACAGTTTCTTGCATCTTCTTCTGTGGATGGTTTTATCGAGGTATGGGATTACATAAGTGGAAAGCTCAAGAAGGATTTGCAATATCAAGCTGATGAATCTTTCATGATGCATGATGATCCTGTCCTCTGCATAGACTTTAGCAGAGATTCAGAGATGCTTGCATCTGGGTCACAAGATGGAAAGATTAAAATCTGGCGTATAAGGACTGGTGTATGCATACGTCGTTTTGATGCACATTCCCAAGGAGTTACAAGTCTTTCCTTCTCTCGGGATGGAAGCCAATTACTGAGTACCTCCTTTGACCAAACTGCAAGAATCCATGGTTTGAAATCTGGGAAATTGTTGAAAGAATTTCGTGGTCACACATCTTATGTAAATCATGCCATCTTTACAAGTGATGGAAGCCGTATTATCACTGCCTCCAGTGATTGTACAGTAAAGGTCTGGGATTCTAAGACGACAGATTGTCTACAAACATTCAAGCCGCCACCTCCGTTAAGGGGAACTGATGCATCAGTCAACTCTATTCACTTGTTCCCCAAAAACACAGAACACATTGTTGTGTGCAACAAGACATCATCTATATACATTATGACACTCCAAGGACAGGTTGTGAAGAGTTTCTCATCCGGTAATAGGGAAGGAGGAGATTTTGTAGCAGCATGTGTATCTACAAAAGGAGATTGGATATACTGCATCGGCGAAGACAAGAAACTGTACTGCTTCAACTATCAATCTGGTGGACTTGAGCATTTCATGATGGTGCATGAGAAGGATGTGATTGGTATAACACACCACCCGCATAGAAACCTCCTTGCTACCTATAGTGAAGATTGCACTATGAAGTTATGGAAGCCCTGA

Protein Analysis

511

Amino Acids

57.72

Weight (kDa)

6.53

Isoelectric Point (pI)

40.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LisH_TPL PF17814 6 - 36 1.5e-14 LisH-like dimerisation domain
Beta-prop_WDR3_2nd PF25172 202 - 326 2.3e-15 WDR3 second beta-propeller domain
WD40_MABP1-WDR62_2nd PF24782 206 - 327 1.8e-09 MABP1/WDR62 second WD40 domain
Beta-prop_EML_2 PF23414 207 - 297 1.4e-12 Echinoderm microtubule-associated protein second beta-propeller
WD40_Prp19 PF24814 210 - 313 3.8e-15 Prp19 WD40 domain
Beta-prop_THOC3 PF25174 212 - 320 1.1e-20 THOC3 beta-propeller domain
WD40_Gbeta PF25391 212 - 337 9.7e-11 G protein beta WD-40 repeat protein
Beta-prop_WDR5 PF25175 212 - 510 4.1e-59 WDR5 beta-propeller domain
WDR55 PF24796 213 - 310 2e-09 WDR55
WD40_CDC20-Fz PF24807 219 - 374 4.4e-23 CDC20/Fizzy WD40 domain
EIF3I PF24805 220 - 304 1.4e-06 EIF3I
WDR90_beta-prop_4th PF23342 221 - 341 6.6e-07 WDR90, 4th beta-propeller
Beta-prop_WDR90_POC16_2nd PF23393 228 - 324 2.7e-06 WDR90/POC16, second beta-propeller
Beta-prop_WDR36-Utp21_1st PF25171 233 - 374 1.7e-11 WDR36/Utp21 first beta-propeller
Beta-prop_TEP1_2nd PF25047 256 - 380 6.5e-15 TEP-1 second beta-propeller
Beta-prop_SPT8 PF23798 258 - 351 8e-06 SPT8 beta propeller
WD40_WDHD1_1st PF24817 258 - 303 6e-07 WDHD1 first WD40 domain
Beta-prop_CAF1B_HIR1 PF24105 259 - 385 2.7e-11 CAF1B/HIR1 beta-propeller domain
Beta-prop_WDR3_1st PF25173 259 - 385 7.7e-30 WDR3 first beta-propeller domain
Beta-prop_EML PF23409 259 - 464 1.1e-08 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR36-Utp21_2nd PF25168 259 - 342 3e-10 WDR36/Utp21 second beta-propeller domain
WD40 PF00400 261 - 290 2.9e-08 WD domain, G-beta repeat
WD40_Prp19 PF24814 263 - 334 1.3e-11 Prp19 WD40 domain
Beta-prop_Aladin PF25460 264 - 368 5.1e-07 Aladin seven-bladed propeller
Beta-prop_ACSF4 PF13570 272 - 476 8.6e-09 Beta-alanine-activating enzyme, beta-propeller
WD40 PF00400 295 - 331 1.6e-07 WD domain, G-beta repeat
WDR55 PF24796 300 - 386 5.2e-10 WDR55
Beta-prop_NWD2_C PF23586 302 - 488 9.6e-07 NWD2 C-terminal beta-propeller
EIF3I PF24805 303 - 509 1.4e-15 EIF3I
WD40_Prp19 PF24814 303 - 434 2.1e-14 Prp19 WD40 domain
Beta-prop_THOC3 PF25174 304 - 509 2.2e-27 THOC3 beta-propeller domain
WD40_WDHD1_1st PF24817 307 - 499 6.7e-16 WDHD1 first WD40 domain
Beta-prop_WDR3_2nd PF25172 308 - 419 1.7e-09 WDR3 second beta-propeller domain
WD40_Gbeta PF25391 316 - 463 1.6e-07 G protein beta WD-40 repeat protein
WD40 PF00400 337 - 375 1.9e-09 WD domain, G-beta repeat
Beta-prop_WDR36-Utp21_2nd PF25168 339 - 389 1.9e-07 WDR36/Utp21 second beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 463
AccB1I GGYRCC 1 cut(s) 463
AccI GTMKAC 2 cut(s) 123, 1144
AciI CCGC 4 cut(s) 346, 383, 1160, 1475
AclWI GGATC 2 cut(s) 374, 785
AcsI RAATTY 2 cut(s) 61, 1025
AcuI CTGAAG 1 cut(s) 29
AfaI GTAC 5 cut(s) 177, 465, 967, 1108, 1394
AfiI CCNNNNNNNGG 4 cut(s) 942, 1175, 1282, 1311
AgsI TTSAA 7 cut(s) 70, 115, 221, 1003, 1021, 1156, 1402
AhdI GACNNNNNGTC 1 cut(s) 1140
AjnI CCWGG 2 cut(s) 206, 535
AluBI AGCT 8 cut(s) 20, 196, 284, 372, 440, 572, 739, 764
AluI AGCT 8 cut(s) 20, 196, 284, 372, 440, 572, 739, 764
Alw26I GTCTC 3 cut(s) 128, 286, 393
AlwI GGATC 2 cut(s) 374, 785
Ama87I CYCGRG 3 cut(s) 21, 279, 942
AoxI GGCC 1 cut(s) 523
ApeKI GCWGC 3 cut(s) 446, 569, 1334
ApoI RAATTY 2 cut(s) 61, 1025
ArsI GACNNNNNNTTYG 2 cut(s) 85, 117
AseI ATTAAT 1 cut(s) 153
Asp700I GAANNNNTTC 1 cut(s) 1295
Asp718I GGTACC 1 cut(s) 463
AspLEI GCGC 2 cut(s) 7, 656
AsuHPI GGTGA 2 cut(s) 316, 657
AvaI CYCGRG 3 cut(s) 21, 279, 942
BaeGI GKGCMC 1 cut(s) 427
BaeI ACNNNNGTAYC 2 cut(s) 447, 480
BanI GGYRCC 1 cut(s) 463
BbsI GAAGAC 3 cut(s) 228, 597, 1386
BbvI GCAGC 3 cut(s) 433, 581, 1346
BceAI ACGGC 2 cut(s) 582, 1065
BciT130I CCWGG 2 cut(s) 208, 537
BcoDI GTCTC 3 cut(s) 128, 286, 393
BfaI CTAG 2 cut(s) 362, 657
BfmI CTRYAG 1 cut(s) 1498
BfoI RGCGCY 1 cut(s) 8
BisI GCNGC 4 cut(s) 447, 570, 1160, 1335
BlsI GCNGC 4 cut(s) 448, 571, 1161, 1336
Bme1390I CCNGG 2 cut(s) 208, 537
BmeRI GACNNNNNGTC 1 cut(s) 1140
BmeT110I CYCGRG 3 cut(s) 21, 279, 942
BmiI GGNNCC 1 cut(s) 465
BmrFI CCNGG 2 cut(s) 208, 537
BmsI GCATC 9 cut(s) 364, 695, 771, 819, 845, 895, 1174, 1196, 1380
BpiI GAAGAC 3 cut(s) 228, 597, 1386
BplI GAGNNNNNCTC 2 cut(s) 283, 315
BpmI CTGGAG 2 cut(s) 519, 1081
BpuEI CTTGAG 4 cut(s) 168, 420, 725, 1442
BsaBI GATNNNNATC 1 cut(s) 863
BsaJI CCNNGG 6 cut(s) 206, 450, 459, 916, 994, 1276
BsaWI WCCGGW 1 cut(s) 1304
BsaXI ACNNNNNCTCC 2 cut(s) 1314, 1344
Bsc4I CCNNNNNNNGG 4 cut(s) 942, 1175, 1282, 1311
Bse1I ACTGG 2 cut(s) 886, 1098
Bse8I GATNNNNATC 1 cut(s) 863
BseBI CCWGG 2 cut(s) 208, 537
BseDI CCNNGG 6 cut(s) 206, 450, 459, 916, 994, 1276
BseGI GGATG 6 cut(s) 354, 598, 706, 952, 1301, 1456
BseJI GATNNNNATC 1 cut(s) 863
BseLI CCNNNNNNNGG 4 cut(s) 942, 1175, 1282, 1311
BseMII CTCAG 2 cut(s) 314, 953
BseNI ACTGG 2 cut(s) 886, 1098
BseRI GAGGAG 1 cut(s) 1335
BseSI GKGCMC 1 cut(s) 427
BseXI GCAGC 3 cut(s) 433, 581, 1346
BseYI CCCAGC 1 cut(s) 192
BshFI GGCC 1 cut(s) 525
BshNI GGYRCC 1 cut(s) 463
BsiHKCI CYCGRG 3 cut(s) 21, 279, 942
BsiSI CCGG 1 cut(s) 1305
BslFI GGGAC 2 cut(s) 182, 577
BslI CCNNNNNNNGG 4 cut(s) 942, 1175, 1282, 1311
BsmAI GTCTC 3 cut(s) 128, 286, 393
BsmBI CGTCTC 1 cut(s) 128
BsmFI GGGAC 2 cut(s) 182, 577
BsmI GAATGC 1 cut(s) 656
BsnI GGCC 1 cut(s) 525
BsoBI CYCGRG 3 cut(s) 21, 279, 942
Bsp1286I GDGCHC 1 cut(s) 427
Bsp1407I TGTACA 1 cut(s) 1106
Bsp143I GATC 2 cut(s) 379, 790
Bsp19I CCATGG 1 cut(s) 994
BspACI CCGC 4 cut(s) 346, 383, 1160, 1475
BspANI GGCC 1 cut(s) 525
BspCNI CTCAG 2 cut(s) 313, 954
BspHI TCATGA 3 cut(s) 315, 777, 1431
BspLI GGNNCC 1 cut(s) 465
BspPI GGATC 2 cut(s) 374, 785
BspT107I GGYRCC 1 cut(s) 463
BsrGI TGTACA 1 cut(s) 1106
BsrI ACTGG 2 cut(s) 886, 1098
BssECI CCNNGG 6 cut(s) 206, 450, 459, 916, 994, 1276
BssMI GATC 2 cut(s) 379, 790
BssT1I CCWWGG 3 cut(s) 916, 994, 1276
Bst2UI CCWGG 2 cut(s) 208, 537
Bst4CI ACNGT 7 cut(s) 121, 460, 477, 546, 678, 1111, 1392
Bst6I CTCTTC 1 cut(s) 1286
BstAPI GCANNNNNTGC 1 cut(s) 431
BstAUI TGTACA 1 cut(s) 1106
BstC8I GCNNGC 3 cut(s) 330, 527, 834
BstDEI CTNAG 3 cut(s) 300, 962, 1128
BstDSI CCRYGG 2 cut(s) 459, 994
BstEII GGTNACC 1 cut(s) 454
BstF5I GGATG 6 cut(s) 354, 598, 706, 952, 1301, 1456
BstH2I RGCGCY 1 cut(s) 8
BstHHI GCGC 2 cut(s) 7, 656
BstKTI GATC 2 cut(s) 382, 793
BstMAI GTCTC 3 cut(s) 128, 286, 393
BstMBI GATC 2 cut(s) 379, 790
BstMWI GCNNNNNNNGC 4 cut(s) 352, 431, 446, 653
BstNI CCWGG 2 cut(s) 208, 537
BstNSI RCATGY 1 cut(s) 1341
BstPI GGTNACC 1 cut(s) 454
BstSCI CCNGG 2 cut(s) 206, 535
BstSFI CTRYAG 1 cut(s) 1498
BstSLI GKGCMC 1 cut(s) 427
BstV1I GCAGC 3 cut(s) 433, 581, 1346
BstV2I GAAGAC 3 cut(s) 228, 597, 1386
BsuRI GGCC 1 cut(s) 525
BtgI CCRYGG 2 cut(s) 459, 994
BtsCI GGATG 6 cut(s) 354, 598, 706, 952, 1301, 1456
BtsI GCAGTG 2 cut(s) 273, 1089
BtsIMutI CAGTG 3 cut(s) 273, 1089, 1105
Cac8I GCNNGC 3 cut(s) 330, 527, 834
CciI TCATGA 3 cut(s) 315, 777, 1431
CfoI GCGC 2 cut(s) 7, 656
CseI GACGC 1 cut(s) 118
Csp6I GTAC 5 cut(s) 176, 464, 966, 1107, 1393
CviQI GTAC 5 cut(s) 176, 464, 966, 1107, 1393
DdeI CTNAG 3 cut(s) 300, 962, 1128
DpnI GATC 2 cut(s) 381, 792
DpnII GATC 2 cut(s) 379, 790
DraI TTTAAA 1 cut(s) 504
DriI GACNNNNNGTC 1 cut(s) 1140
Eam1104I CTCTTC 1 cut(s) 1286
Eam1105I GACNNNNNGTC 1 cut(s) 1140
EarI CTCTTC 1 cut(s) 1286
Eco130I CCWWGG 3 cut(s) 916, 994, 1276
Eco147I AGGCCT 1 cut(s) 525
Eco57I CTGAAG 1 cut(s) 29
Eco88I CYCGRG 3 cut(s) 21, 279, 942
Eco91I GGTNACC 1 cut(s) 454
EcoO65I GGTNACC 1 cut(s) 454
EcoRII CCWGG 2 cut(s) 206, 535
EcoT14I CCWWGG 3 cut(s) 916, 994, 1276
EcoT22I ATGCAT 3 cut(s) 786, 893, 1189
ErhI CCWWGG 3 cut(s) 916, 994, 1276
Esp3I CGTCTC 1 cut(s) 128
FaqI GGGAC 2 cut(s) 182, 577
FauI CCCGC 1 cut(s) 1482
FblI GTMKAC 2 cut(s) 123, 1144
Fnu4HI GCNGC 4 cut(s) 447, 570, 1160, 1335
FokI GGATG 6 cut(s) 361, 585, 713, 959, 1288, 1463
Fsp4HI GCNGC 4 cut(s) 447, 570, 1160, 1335
FspBI CTAG 2 cut(s) 362, 657
GlaI GCGC 2 cut(s) 6, 655
GluI GCNGC 4 cut(s) 447, 570, 1160, 1335
GsaI CCCAGC 1 cut(s) 196
GsuI CTGGAG 2 cut(s) 519, 1081
HaeII RGCGCY 1 cut(s) 8
HaeIII GGCC 1 cut(s) 525
HapII CCGG 1 cut(s) 1305
HgaI GACGC 1 cut(s) 118
HhaI GCGC 2 cut(s) 7, 656
Hin6I GCGC 2 cut(s) 5, 654
HinP1I GCGC 2 cut(s) 5, 654
HincII GTYRAC 1 cut(s) 1195
HindII GTYRAC 1 cut(s) 1195
HinfI GANTC 9 cut(s) 70, 170, 401, 640, 660, 770, 821, 990, 1124
HpaII CCGG 1 cut(s) 1305
HphI GGTGA 2 cut(s) 316, 657
Hpy166II GTNNAC 4 cut(s) 124, 1145, 1195, 1418
Hpy188I TCNGA 4 cut(s) 75, 148, 368, 826
Hpy188III TCNNGA 9 cut(s) 23, 82, 133, 263, 316, 742, 778, 944, 1432
Hpy8I GTNNAC 4 cut(s) 124, 1145, 1195, 1418
Hpy99I CGWCG 2 cut(s) 134, 900
HpyAV CCTTC 5 cut(s) 480, 739, 946, 1310, 1441
HpyCH4III ACNGT 7 cut(s) 121, 460, 477, 546, 678, 1111, 1392
HpyCH4IV ACGT 2 cut(s) 137, 895
HpyF10VI GCNNNNNNNGC 4 cut(s) 352, 431, 446, 653
HpyF3I CTNAG 3 cut(s) 300, 962, 1128
HpySE526I ACGT 2 cut(s) 137, 895
HspAI GCGC 2 cut(s) 5, 654
KpnI GGTACC 1 cut(s) 467
Kzo9I GATC 2 cut(s) 379, 790
LmnI GCTCC 3 cut(s) 325, 352, 534
Lsp1109I GCAGC 3 cut(s) 433, 581, 1346
LweI GCATC 9 cut(s) 364, 695, 771, 819, 845, 895, 1174, 1196, 1380
MaeI CTAG 2 cut(s) 362, 657
MaeII ACGT 2 cut(s) 137, 895
MaeIII GTNAC 4 cut(s) 454, 843, 922, 1034
MalI GATC 2 cut(s) 381, 792
MboI GATC 2 cut(s) 379, 790
MboII GAAGA 9 cut(s) 174, 233, 350, 602, 681, 684, 1303, 1391, 1517
MhlI GDGCHC 1 cut(s) 427
MluCI AATT 4 cut(s) 61, 956, 1013, 1025
MlyI GAGTC 3 cut(s) 164, 395, 649
MmeI TCCRAC 1 cut(s) 249
Mph1103I ATGCAT 3 cut(s) 786, 893, 1189
MroXI GAANNNNTTC 1 cut(s) 1295
MseI TTAA 6 cut(s) 153, 198, 479, 503, 861, 1172
MslI CAYNNNNRTG 3 cut(s) 603, 1235, 1436
MspI CCGG 1 cut(s) 1305
MspR9I CCNGG 2 cut(s) 208, 537
Mva1269I GAATGC 1 cut(s) 656
MvaI CCWGG 2 cut(s) 208, 537
MwoI GCNNNNNNNGC 4 cut(s) 352, 431, 446, 653
NcoI CCATGG 1 cut(s) 994
NdeII GATC 2 cut(s) 379, 790
NlaIV GGNNCC 1 cut(s) 465
NmeAIII GCCGAG 1 cut(s) 475
NmuCI GTSAC 2 cut(s) 843, 1034
NsiI ATGCAT 3 cut(s) 786, 893, 1189
NspI RCATGY 1 cut(s) 1341
PaeR7I CTCGAG 1 cut(s) 21
PagI TCATGA 3 cut(s) 315, 777, 1431
PceI AGGCCT 1 cut(s) 525
PctI GAATGC 1 cut(s) 656
PdmI GAANNNNTTC 1 cut(s) 1295
PfeI GAWTC 6 cut(s) 70, 660, 770, 821, 990, 1124
PflFI GACNNNGTC 1 cut(s) 128
PfoI TCCNGGA 1 cut(s) 535
PkrI GCNGC 4 cut(s) 448, 571, 1161, 1336
PleI GAGTC 3 cut(s) 164, 395, 648
PpsI GAGTC 3 cut(s) 164, 395, 648
PshBI ATTAAT 1 cut(s) 153
Psp6I CCWGG 2 cut(s) 206, 535
PspEI GGTNACC 1 cut(s) 454
PspFI CCCAGC 1 cut(s) 192
PspGI CCWGG 2 cut(s) 206, 535
PspN4I GGNNCC 1 cut(s) 465
PsyI GACNNNGTC 1 cut(s) 128
RsaI GTAC 5 cut(s) 177, 465, 967, 1108, 1394
RsaNI GTAC 5 cut(s) 176, 464, 966, 1107, 1393
RseI CAYNNNNRTG 3 cut(s) 603, 1235, 1436
SaqAI TTAA 6 cut(s) 153, 198, 479, 503, 861, 1172
SatI GCNGC 4 cut(s) 447, 570, 1160, 1335
Sau3AI GATC 2 cut(s) 379, 790
SchI GAGTC 3 cut(s) 164, 395, 649
ScrFI CCNGG 2 cut(s) 208, 537
SduI GDGCHC 1 cut(s) 427
SfaNI GCATC 9 cut(s) 364, 695, 771, 819, 845, 895, 1174, 1196, 1380
SfcI CTRYAG 1 cut(s) 1498
Sfr274I CTCGAG 1 cut(s) 21
SlaI CTCGAG 1 cut(s) 21
SmiMI CAYNNNNRTG 3 cut(s) 603, 1235, 1436
SmlI CTYRAG 5 cut(s) 21, 183, 435, 740, 1421
SmoI CTYRAG 5 cut(s) 21, 183, 435, 740, 1421
Sse9I AATT 4 cut(s) 61, 956, 1013, 1025
SseBI AGGCCT 1 cut(s) 525
SsiI CCGC 4 cut(s) 346, 383, 1160, 1475
SspMI CTAG 2 cut(s) 362, 657
StuI AGGCCT 1 cut(s) 525
StyD4I CCNGG 2 cut(s) 206, 535
StyI CCWWGG 3 cut(s) 916, 994, 1276
TaaI ACNGT 7 cut(s) 121, 460, 477, 546, 678, 1111, 1392
TaiI ACGT 2 cut(s) 140, 898
TaqI TCGA 6 cut(s) 9, 15, 22, 132, 262, 711
TasI AATT 4 cut(s) 61, 956, 1013, 1025
TatI WGTACW 2 cut(s) 1106, 1392
TauI GCSGC 1 cut(s) 1162
TfiI GAWTC 6 cut(s) 70, 660, 770, 821, 990, 1124
Tru1I TTAA 6 cut(s) 153, 198, 479, 503, 861, 1172
Tru9I TTAA 6 cut(s) 153, 198, 479, 503, 861, 1172
TscAI CASTG 3 cut(s) 280, 1096, 1105
TseFI GTSAC 2 cut(s) 843, 1034
TseI GCWGC 3 cut(s) 446, 569, 1334
Tsp45I GTSAC 2 cut(s) 843, 1034
TspDTI ATGAA 7 cut(s) 246, 332, 590, 766, 783, 1420, 1532
TspGWI ACGGA 1 cut(s) 1158
TspRI CASTG 3 cut(s) 280, 1096, 1105
Tth111I GACNNNGTC 1 cut(s) 128
VspI ATTAAT 1 cut(s) 153
XapI RAATTY 2 cut(s) 61, 1025
XceI RCATGY 1 cut(s) 1341
XhoI CTCGAG 1 cut(s) 21
XmiI GTMKAC 2 cut(s) 123, 1144
XmnI GAANNNNTTC 1 cut(s) 1295
XspI CTAG 2 cut(s) 362, 657
Zsp2I ATGCAT 3 cut(s) 786, 893, 1189
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.