Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr1

Orthogroup ID

OG0008538

Physical Location & Seq

Genomic Coordinates Reverse (-)

16122903 .. 16134328

Gene Structure 11426 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000001479

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1542 bp

ATGTCTACCTTGGAAATCGAAGCTCGAGATGTCATCAAGATTGTGCTTCAATTTTGCAAAGAGAATTCTCTGCACCAAACGTTTCAGACATTACAGAGCGAGTGCCAGGTTTCGCTGAACACTGTTGATAGTGTGGAGACGTTTGTAGCTGATATAAATAGTGGAAGGTGGGATGCAGTTTTGCCTCAAGTGGCTCAGCTTAAGCTGTCTAGGAATAAATTGGAGGATTTGTATGAGCAGATTGTACTGGAAATGATTGAACTTCGAGAAGTAGAGACTGCTCGTTCTATTTTAAGAAACACTCAGGTAATGGGTGTAATGAAGCAAGAACAACCAGAAAGATACTTGCGACTGGAGCATCTCCATGCCAGAACTTATTTTGATCCAAATGAGGCTTATCAAGATTCGACAAAAGAGAAACGGCGTGCGCAGATTGCTCAAGCACTTGCTGCAGAAGTTACTGTGGTGCCACCTTCAAGATTAATGGCTCTAATTGGTCAGGCTCTAAAGTGGCAGCAGCACCAAGGATTACTTCCTCCAGGAACACAGTTTGACTTATTTAGGGGAACGGCAGCCATGAAACAGGATGTGGATGACTTGTATCCAACAACTCTTCAACACACAATTAAGTTTGGGACCAAAAGTCATGCAGAATGTGCTCGGTTCTCACCAGATGGCCAGTTTCTTGTCTCTACATCTTTTGATGGATTTATCGAGGTATGGGATTACATAAGTGGGAAACTCAAGAAGGATCTTCAATACCAGGCTGATGAAGCTTTCATGCTCCATGACGATCCAGTCCTGTGTGCTGATTTTAGCAGAGATTCAGAGATGCTTGCATCTGGGGATCAATGTGGCAAGATCAAAGTTTGGCGTATTAGGACTGGTCAGTGCTTGCGGCGTCTTGAACATGCACATTCGAAGGGTGTTACCAGTGTTACCTTCTCTCGAGATGGAAGCCAAATACTTAGTACATCATTTGACAGCACTGCAAGGATCCATGGCTTAAAATCTGGGAAGCTGTTGAAAGAATTTCGCGGTCATTCATCTTATGTTAATAATGCAATCTTTACAAGTGATGGTGCCCGTGTAATTACCGCATCTAGTGATTGCACGGTCAAGGTCTGGGATTCGAAGACTGCCGACTGTCTACAGACATTTAAACCACCTCCTCCTTTAAGGGGGGGTGATGCTTCGGTGAATTCTGTTCATCTTTTCCCAAAAAGCAATGATCACATCGTTGTATGCAATAAGACTTCATCGATATATATCATGACACTTCAAGGACAGGTTGTGAAGAGCTTTTCATCTGGTAAAAGAGAAGGTGGAGATTTTATGGCTGCATGTGTGTCACCAAAAGGGGAATGGATTTACTGTGTTGGTGAAGACAGGAATCTGTATTGTTTCAGTTACCAATCTGGAAAGTTAGAGCATTTAATGAAGATCCATGAAAAGGATGTTATTGGTCTCGCTCATCATCCTCACAGGAATCTTCTTGCCACATTTGGTGAAGACTGCACAATGAAGTTATGGAAGCCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000151 GO:0000381 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0008380 GO:0009987 GO:0010467 GO:0010468 GO:0016070 GO:0016604 GO:0016607 GO:0019219 GO:0019222 GO:0031323 GO:0031461 GO:0031974 GO:0031981 GO:0032991 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043484 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0046483 GO:0048024 GO:0050684 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0070013 GO:0071704 GO:0080008 GO:0080090 GO:0090304 GO:1901360 GO:1902494 GO:1903311 GO:1990234

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

WD40

Protein Analysis

514

Amino Acids

57.79

Weight (kDa)

6.81

Isoelectric Point (pI)

42.18

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LisH_TPL	PF17814	7 - 37	3.2e-15	LisH-like dimerisation domain
Beta-prop_WDR3_2nd	PF25172	203 - 325	1.2e-10	WDR3 second beta-propeller domain
Beta-prop_THOC3	PF25174	206 - 304	6.4e-12	THOC3 beta-propeller domain
WD40_Prp19	PF24814	212 - 320	5.6e-13	Prp19 WD40 domain
Beta-prop_EML_2	PF23414	213 - 299	6.4e-09	Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR5	PF25175	214 - 512	1.9e-52	WDR5 beta-propeller domain
WDR55	PF24796	215 - 303	1.5e-08	WDR55
WD40_Gbeta	PF25391	216 - 339	7e-07	G protein beta WD-40 repeat protein
Beta-prop_EML	PF23409	220 - 326	3.2e-08	Echinoderm microtubule-associated protein first beta-propeller
WDR90_beta-prop_4th	PF23342	222 - 345	2.5e-06	WDR90, 4th beta-propeller
Beta-prop_TEP1_2nd	PF25047	258 - 380	1.8e-13	TEP-1 second beta-propeller
Beta-prop_CAF1B_HIR1	PF24105	261 - 386	5.8e-09	CAF1B/HIR1 beta-propeller domain
Beta-prop_WDR3_1st	PF25173	263 - 387	2.3e-24	WDR3 first beta-propeller domain
Beta-prop_WDR36-Utp21_2nd	PF25168	263 - 345	6.8e-07	WDR36/Utp21 second beta-propeller domain
WD40_CDC20-Fz	PF24807	263 - 388	1.2e-13	CDC20/Fizzy WD40 domain
Beta-prop_WDR36-Utp21_1st	PF25171	264 - 328	7.3e-06	WDR36/Utp21 first beta-propeller
Beta-prop_ACSF4	PF13570	274 - 482	2.8e-07	Beta-alanine-activating enzyme, beta-propeller
WD40	PF00400	296 - 333	3.3e-06	WD domain, G-beta repeat
EIF3I	PF24805	304 - 511	1e-15	EIF3I
WDR55	PF24796	304 - 388	2.9e-10	WDR55
WD40_Prp19	PF24814	304 - 449	8.8e-15	Prp19 WD40 domain
Beta-prop_THOC3	PF25174	306 - 511	4.8e-28	THOC3 beta-propeller domain
WD40_Gbeta	PF25391	308 - 413	3.5e-08	G protein beta WD-40 repeat protein
WD40_WDHD1_1st	PF24817	309 - 501	6.8e-17	WDHD1 first WD40 domain
Beta-prop_WDR3_2nd	PF25172	310 - 388	8.3e-09	WDR3 second beta-propeller domain
WD40	PF00400	339 - 377	1.6e-08	WD domain, G-beta repeat
Beta-prop_WDR36-Utp21_2nd	PF25168	341 - 390	1.4e-07	WDR36/Utp21 second beta-propeller domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0008538)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G73720
fragaria_vesca	FvH4_5g32860
malus_domestica	MD08G1215900.v1.1 MD15G1400300.v1.1
prunus_persica	Prupe.1G547900_v2.0.a1
pyrus_communis	pycom08g18700 pycom15g35890
rosa_chinensis	RchiOBHm_Chr6g0269341 RchiOBHm_Chr7g0230731
rosa_laevigata	RLG00000001479
rosa_multiflora	Rmu_sc0007883.1_g000005
rosa_roxburghii	Rroxscaffold_3G00229780
rosa_rugosa	Rorug07G0262400
rosa_samantha	Rh3AG002100 Rh3BG001600 Rh3CG001900 Rh3DG002000 Rh7AG414100 Rh7BG391300 Rh7CG433000 Rh7DG409700
rosa_wichuraiana	Rw7G034230 Rw7G034380

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	797
Acc16I	TGCGCA	1 cut(s)	429
AccB1I	GGYRCC	2 cut(s)	466, 1082
AccI	GTMKAC	2 cut(s)	5, 1150
AccII	CGCG	1 cut(s)	1038
AciI	CCGC	3 cut(s)	898, 1038, 1098
AclI	AACGTT	1 cut(s)	80
AclWI	GGATC	7 cut(s)	377, 759, 788, 855, 991, 1004, 1438
AcoI	YGGCCR	1 cut(s)	676
AcsI	RAATTY	3 cut(s)	64, 1031, 1201
AcyI	GRCGYC	1 cut(s)	901
AfaI	GTAC	2 cut(s)	246, 973
AfiI	CCNNNNNNNGG	2 cut(s)	1181, 1453
AflII	CTTAAG	1 cut(s)	200
AgsI	TTSAA	8 cut(s)	50, 260, 477, 617, 758, 908, 1027, 1283
AhdI	GACNNNNNGTC	1 cut(s)	642
AjnI	CCWGG	3 cut(s)	105, 538, 762
AjuI	GAANNNNNNNTTGG	2 cut(s)	516, 548
AluBI	AGCT	7 cut(s)	23, 149, 199, 205, 776, 1021, 1302
AluI	AGCT	7 cut(s)	23, 149, 199, 205, 776, 1021, 1302
Alw21I	GWGCWC	1 cut(s)	661
Alw26I	GTCTC	4 cut(s)	131, 269, 694, 1472
AlwI	GGATC	7 cut(s)	377, 759, 788, 855, 991, 1004, 1438
Ama87I	CYCGRG	2 cut(s)	24, 948
AoxI	GGCC	1 cut(s)	676
ApeKI	GCWGC	5 cut(s)	449, 514, 517, 572, 1340
ApoI	RAATTY	3 cut(s)	64, 1031, 1201
AseI	ATTAAT	1 cut(s)	482
AspLEI	GCGC	1 cut(s)	430
AspS9I	GGNCC	1 cut(s)	636
AsuHPI	GGTGA	6 cut(s)	660, 1199, 1210, 1344, 1394, 1520
AsuII	TTCGAA	2 cut(s)	920, 1133
AvaI	CYCGRG	2 cut(s)	24, 948
AvaII	GGWCC	1 cut(s)	636
BaeGI	GKGCMC	1 cut(s)	1087
BalI	TGGCCA	1 cut(s)	678
BamHI	GGATCC	1 cut(s)	996
BanI	GGYRCC	2 cut(s)	466, 1082
BbsI	GAAGAC	3 cut(s)	1142, 1392, 1518
Bbv12I	GWGCWC	1 cut(s)	661
BbvI	GCAGC	5 cut(s)	436, 526, 529, 584, 1327
BccI	CCATC	4 cut(s)	668, 698, 947, 1073
BceAI	ACGGC	2 cut(s)	437, 585
BciT130I	CCWGG	3 cut(s)	107, 540, 764
BciVI	GTATCC	1 cut(s)	612
BclI	TGATCA	1 cut(s)	1231
BcoDI	GTCTC	4 cut(s)	131, 269, 694, 1472
BfaI	CTAG	2 cut(s)	210, 1104
BfmI	CTRYAG	2 cut(s)	450, 1151
BfrI	CTTAAG	1 cut(s)	200
BfuI	GTATCC	1 cut(s)	612
BisI	GCNGC	6 cut(s)	450, 515, 518, 573, 899, 1341
BlpI	GCTNAGC	1 cut(s)	195
BlsI	GCNGC	6 cut(s)	451, 516, 519, 574, 900, 1342
Bme1390I	CCNGG	3 cut(s)	107, 540, 764
Bme18I	GGWCC	1 cut(s)	636
BmeRI	GACNNNNNGTC	1 cut(s)	642
BmeT110I	CYCGRG	2 cut(s)	24, 948
BmgT120I	GGNCC	1 cut(s)	636
BmiI	GGNNCC	4 cut(s)	468, 637, 998, 1084
BmrFI	CCNGG	3 cut(s)	107, 540, 764
BmsI	GCATC	6 cut(s)	163, 367, 822, 848, 1109, 1180
BpiI	GAAGAC	3 cut(s)	1142, 1392, 1518
BpmI	CTGGAG	2 cut(s)	374, 522
Bpu1102I	GCTNAGC	1 cut(s)	195
Bpu14I	TTCGAA	2 cut(s)	920, 1133
BpuEI	CTTGAG	3 cut(s)	171, 423, 728
Bsa29I	ATCGAT	1 cut(s)	1262
BsaBI	GATNNNNATC	1 cut(s)	1268
BsaHI	GRCGYC	1 cut(s)	901
BsaI	GGTCTC	1 cut(s)	1472
BsaJI	CCNNGG	3 cut(s)	9, 523, 1000
BsaXI	ACNNNNNCTCC	2 cut(s)	215, 245
Bsc4I	CCNNNNNNNGG	2 cut(s)	1181, 1453
Bse1I	ACTGG	6 cut(s)	252, 357, 679, 797, 889, 933
Bse3DI	GCAATG	1 cut(s)	1234
Bse8I	GATNNNNATC	1 cut(s)	1268
BseBI	CCWGG	3 cut(s)	107, 540, 764
BseCI	ATCGAT	1 cut(s)	1262
BseDI	CCNNGG	3 cut(s)	9, 523, 1000
BseGI	GGATG	5 cut(s)	178, 592, 598, 1462, 1477
BseJI	GATNNNNATC	1 cut(s)	1268
BseLI	CCNNNNNNNGG	2 cut(s)	1181, 1453
BseMI	GCAATG	1 cut(s)	1234
BseMII	CTCAG	2 cut(s)	209, 317
BseNI	ACTGG	6 cut(s)	252, 357, 679, 797, 889, 933
BseRI	GAGGAG	1 cut(s)	1161
BseSI	GKGCMC	1 cut(s)	1087
BseXI	GCAGC	5 cut(s)	436, 526, 529, 584, 1327
BsgI	GTGCAG	2 cut(s)	56, 1501
Bsh1236I	CGCG	1 cut(s)	1038
BshFI	GGCC	1 cut(s)	678
BshNI	GGYRCC	2 cut(s)	466, 1082
BshVI	ATCGAT	1 cut(s)	1262
BsiHKAI	GWGCWC	1 cut(s)	661
BsiHKCI	CYCGRG	2 cut(s)	24, 948
BslFI	GGGAC	1 cut(s)	649
BslI	CCNNNNNNNGG	2 cut(s)	1181, 1453
BsmAI	GTCTC	4 cut(s)	131, 269, 694, 1472
BsmBI	CGTCTC	1 cut(s)	131
BsmFI	GGGAC	1 cut(s)	649
BsnI	GGCC	1 cut(s)	678
Bso31I	GGTCTC	1 cut(s)	1472
BsoBI	CYCGRG	2 cut(s)	24, 948
Bsp119I	TTCGAA	2 cut(s)	920, 1133
Bsp1286I	GDGCHC	2 cut(s)	661, 1087
Bsp143I	GATC	8 cut(s)	382, 751, 793, 847, 861, 996, 1231, 1443
Bsp1720I	GCTNAGC	1 cut(s)	195
Bsp19I	CCATGG	1 cut(s)	1000
BspACI	CCGC	3 cut(s)	898, 1038, 1098
BspANI	GGCC	1 cut(s)	678
BspCNI	CTCAG	2 cut(s)	208, 316
BspDI	ATCGAT	1 cut(s)	1262
BspFNI	CGCG	1 cut(s)	1038
BspHI	TCATGA	1 cut(s)	1272
BspLI	GGNNCC	4 cut(s)	468, 637, 998, 1084
BspMAI	CTGCAG	1 cut(s)	454
BspPI	GGATC	7 cut(s)	377, 759, 788, 855, 991, 1004, 1438
BspQI	GCTCTTC	1 cut(s)	1292
BspT104I	TTCGAA	2 cut(s)	920, 1133
BspT107I	GGYRCC	2 cut(s)	466, 1082
BspTI	CTTAAG	1 cut(s)	200
BspTNI	GGTCTC	1 cut(s)	1472
BsrDI	GCAATG	1 cut(s)	1234
BsrI	ACTGG	6 cut(s)	252, 357, 679, 797, 889, 933
BssECI	CCNNGG	3 cut(s)	9, 523, 1000
BssMI	GATC	8 cut(s)	382, 751, 793, 847, 861, 996, 1231, 1443
BssNI	GRCGYC	1 cut(s)	901
BssT1I	CCWWGG	3 cut(s)	9, 523, 1000
Bst2UI	CCWGG	3 cut(s)	107, 540, 764
Bst4CI	ACNGT	6 cut(s)	124, 463, 549, 1117, 1148, 1376
Bst6I	CTCTTC	2 cut(s)	618, 1292
BstACI	GRCGYC	1 cut(s)	901
BstAFI	CTTAAG	1 cut(s)	200
BstAPI	GCANNNNNTGC	2 cut(s)	449, 656
BstBI	TTCGAA	2 cut(s)	920, 1133
BstC8I	GCNNGC	3 cut(s)	426, 837, 896
BstDEI	CTNAG	3 cut(s)	195, 303, 968
BstDSI	CCRYGG	1 cut(s)	1000
BstF5I	GGATG	5 cut(s)	178, 592, 598, 1462, 1477
BstFNI	CGCG	1 cut(s)	1038
BstHHI	GCGC	1 cut(s)	430
BstKTI	GATC	8 cut(s)	385, 754, 796, 850, 864, 999, 1234, 1446
BstMAI	GTCTC	4 cut(s)	131, 269, 694, 1472
BstMBI	GATC	8 cut(s)	382, 751, 793, 847, 861, 996, 1231, 1443
BstMWI	GCNNNNNNNGC	5 cut(s)	355, 434, 449, 656, 773
BstNI	CCWGG	3 cut(s)	107, 540, 764
BstNSI	RCATGY	2 cut(s)	914, 1347
BstSCI	CCNGG	3 cut(s)	105, 538, 762
BstSFI	CTRYAG	2 cut(s)	450, 1151
BstSLI	GKGCMC	1 cut(s)	1087
BstUI	CGCG	1 cut(s)	1038
BstV1I	GCAGC	5 cut(s)	436, 526, 529, 584, 1327
BstV2I	GAAGAC	3 cut(s)	1142, 1392, 1518
BstX2I	RGATCY	3 cut(s)	751, 996, 1443
BstYI	RGATCY	3 cut(s)	751, 996, 1443
Bsu15I	ATCGAT	1 cut(s)	1262
BsuI	GTATCC	1 cut(s)	612
BsuRI	GGCC	1 cut(s)	678
BsuTUI	ATCGAT	1 cut(s)	1262
BtgI	CCRYGG	1 cut(s)	1000
BtsCI	GGATG	5 cut(s)	178, 592, 598, 1462, 1477
BtsI	GCAGTG	1 cut(s)	987
BtsIMutI	CAGTG	4 cut(s)	120, 896, 940, 987
Cac8I	GCNNGC	3 cut(s)	426, 837, 896
CciI	TCATGA	1 cut(s)	1272
CfoI	GCGC	1 cut(s)	430
Cfr13I	GGNCC	1 cut(s)	636
ClaI	ATCGAT	1 cut(s)	1262
CseI	GACGC	1 cut(s)	890
Csp6I	GTAC	2 cut(s)	245, 972
CviQI	GTAC	2 cut(s)	245, 972
DdeI	CTNAG	3 cut(s)	195, 303, 968
DpnI	GATC	8 cut(s)	384, 753, 795, 849, 863, 998, 1233, 1445
DpnII	GATC	8 cut(s)	382, 751, 793, 847, 861, 996, 1231, 1443
DraI	TTTAAA	1 cut(s)	1162
DrdI	GACNNNNNNGTC	1 cut(s)	797
DriI	GACNNNNNGTC	1 cut(s)	642
DseDI	GACNNNNNNGTC	1 cut(s)	797
EaeI	YGGCCR	1 cut(s)	676
Eam1104I	CTCTTC	2 cut(s)	618, 1292
Eam1105I	GACNNNNNGTC	1 cut(s)	642
EarI	CTCTTC	2 cut(s)	618, 1292
Eco130I	CCWWGG	3 cut(s)	9, 523, 1000
Eco31I	GGTCTC	1 cut(s)	1472
Eco47I	GGWCC	1 cut(s)	636
Eco88I	CYCGRG	2 cut(s)	24, 948
EcoRI	GAATTC	2 cut(s)	64, 1201
EcoRII	CCWGG	3 cut(s)	105, 538, 762
EcoT14I	CCWWGG	3 cut(s)	9, 523, 1000
ErhI	CCWWGG	3 cut(s)	9, 523, 1000
Esp3I	CGTCTC	1 cut(s)	131
FalI	AAGNNNNNCTT	2 cut(s)	516, 548
FaqI	GGGAC	1 cut(s)	649
FbaI	TGATCA	1 cut(s)	1231
FblI	GTMKAC	2 cut(s)	5, 1150
Fnu4HI	GCNGC	6 cut(s)	450, 515, 518, 573, 899, 1341
FokI	GGATG	5 cut(s)	185, 599, 605, 1464, 1469
Fsp4HI	GCNGC	6 cut(s)	450, 515, 518, 573, 899, 1341
FspBI	CTAG	2 cut(s)	210, 1104
FspI	TGCGCA	1 cut(s)	429
GlaI	GCGC	1 cut(s)	429
GluI	GCNGC	6 cut(s)	450, 515, 518, 573, 899, 1341
GsuI	CTGGAG	2 cut(s)	374, 522
HaeIII	GGCC	1 cut(s)	678
HgaI	GACGC	1 cut(s)	890
HhaI	GCGC	1 cut(s)	430
Hin1I	GRCGYC	1 cut(s)	901
Hin6I	GCGC	1 cut(s)	428
HinP1I	GCGC	1 cut(s)	428
HindIII	AAGCTT	1 cut(s)	774
HinfI	GANTC	5 cut(s)	404, 824, 1130, 1393, 1489
HphI	GGTGA	6 cut(s)	660, 1199, 1210, 1344, 1394, 1520
Hpy166II	GTNNAC	2 cut(s)	6, 1151
Hpy188I	TCNGA	2 cut(s)	87, 829
Hpy8I	GTNNAC	2 cut(s)	6, 1151
HpyAV	CCTTC	6 cut(s)	159, 483, 742, 916, 952, 1316
HpyCH4III	ACNGT	6 cut(s)	124, 463, 549, 1117, 1148, 1376
HpyCH4IV	ACGT	2 cut(s)	80, 140
HpyF10VI	GCNNNNNNNGC	5 cut(s)	355, 434, 449, 656, 773
HpyF3I	CTNAG	3 cut(s)	195, 303, 968
HpySE526I	ACGT	2 cut(s)	80, 140
Hsp92I	GRCGYC	1 cut(s)	901
HspAI	GCGC	1 cut(s)	428
Ksp22I	TGATCA	1 cut(s)	1231
Kzo9I	GATC	8 cut(s)	382, 751, 793, 847, 861, 996, 1231, 1443
LguI	GCTCTTC	1 cut(s)	1292
LmnI	GCTCC	2 cut(s)	355, 789
Lsp1109I	GCAGC	5 cut(s)	436, 526, 529, 584, 1327
LweI	GCATC	6 cut(s)	163, 367, 822, 848, 1109, 1180
MaeI	CTAG	2 cut(s)	210, 1104
MaeII	ACGT	2 cut(s)	80, 140
MaeIII	GTNAC	5 cut(s)	457, 928, 937, 1350, 1409
MalI	GATC	8 cut(s)	384, 753, 795, 849, 863, 998, 1233, 1445
MboI	GATC	8 cut(s)	382, 751, 793, 847, 861, 996, 1231, 1443
MboII	GAAGA	8 cut(s)	605, 746, 1147, 1309, 1397, 1453, 1484, 1523
MflI	RGATCY	3 cut(s)	751, 996, 1443
MhlI	GDGCHC	2 cut(s)	661, 1087
MlsI	TGGCCA	1 cut(s)	678
MluCI	AATT	8 cut(s)	50, 64, 218, 492, 624, 1031, 1092, 1201
MluNI	TGGCCA	1 cut(s)	678
MmeI	TCCRAC	1 cut(s)	629
MnlI	CCTC	8 cut(s)	195, 217, 385, 546, 709, 1179, 1182, 1491
Mox20I	TGGCCA	1 cut(s)	678
MscI	TGGCCA	1 cut(s)	678
MseI	TTAA	9 cut(s)	201, 293, 482, 627, 1007, 1056, 1161, 1178, 1436
MslI	CAYNNNNRTG	1 cut(s)	363
Msp20I	TGGCCA	1 cut(s)	678
MspCI	CTTAAG	1 cut(s)	200
MspR9I	CCNGG	3 cut(s)	107, 540, 764
MvaI	CCWGG	3 cut(s)	107, 540, 764
MvnI	CGCG	1 cut(s)	1038
MwoI	GCNNNNNNNGC	5 cut(s)	355, 434, 449, 656, 773
NcoI	CCATGG	1 cut(s)	1000
NdeII	GATC	8 cut(s)	382, 751, 793, 847, 861, 996, 1231, 1443
NlaIV	GGNNCC	4 cut(s)	468, 637, 998, 1084
NmuCI	GTSAC	1 cut(s)	1350
NsbI	TGCGCA	1 cut(s)	429
NspI	RCATGY	2 cut(s)	914, 1347
NspV	TTCGAA	2 cut(s)	920, 1133
PaeR7I	CTCGAG	2 cut(s)	24, 948
PagI	TCATGA	1 cut(s)	1272
PciSI	GCTCTTC	1 cut(s)	1292
PfeI	GAWTC	5 cut(s)	404, 824, 1130, 1393, 1489
PfoI	TCCNGGA	1 cut(s)	538
PkrI	GCNGC	6 cut(s)	451, 516, 519, 574, 900, 1342
PshBI	ATTAAT	1 cut(s)	482
Psp1406I	AACGTT	1 cut(s)	80
Psp6I	CCWGG	3 cut(s)	105, 538, 762
PspGI	CCWGG	3 cut(s)	105, 538, 762
PspN4I	GGNNCC	4 cut(s)	468, 637, 998, 1084
PspPI	GGNCC	1 cut(s)	636
PstI	CTGCAG	1 cut(s)	454
PsuI	RGATCY	3 cut(s)	751, 996, 1443
RsaI	GTAC	2 cut(s)	246, 973
RsaNI	GTAC	2 cut(s)	245, 972
RseI	CAYNNNNRTG	1 cut(s)	363
SapI	GCTCTTC	1 cut(s)	1292
SaqAI	TTAA	9 cut(s)	201, 293, 482, 627, 1007, 1056, 1161, 1178, 1436
SatI	GCNGC	6 cut(s)	450, 515, 518, 573, 899, 1341
Sau3AI	GATC	8 cut(s)	382, 751, 793, 847, 861, 996, 1231, 1443
Sau96I	GGNCC	1 cut(s)	636
ScrFI	CCNGG	3 cut(s)	107, 540, 764
SduI	GDGCHC	2 cut(s)	661, 1087
SfaNI	GCATC	6 cut(s)	163, 367, 822, 848, 1109, 1180
SfcI	CTRYAG	2 cut(s)	450, 1151
Sfr274I	CTCGAG	2 cut(s)	24, 948
SfuI	TTCGAA	2 cut(s)	920, 1133
SinI	GGWCC	1 cut(s)	636
SlaI	CTCGAG	2 cut(s)	24, 948
SmiMI	CAYNNNNRTG	1 cut(s)	363
SmlI	CTYRAG	6 cut(s)	24, 186, 200, 438, 743, 948
SmoI	CTYRAG	6 cut(s)	24, 186, 200, 438, 743, 948
Sse9I	AATT	8 cut(s)	50, 64, 218, 492, 624, 1031, 1092, 1201
SsiI	CCGC	3 cut(s)	898, 1038, 1098
SspMI	CTAG	2 cut(s)	210, 1104
StyD4I	CCNGG	3 cut(s)	105, 538, 762
StyI	CCWWGG	3 cut(s)	9, 523, 1000
TaaI	ACNGT	6 cut(s)	124, 463, 549, 1117, 1148, 1376
TaiI	ACGT	2 cut(s)	83, 143
TaqI	TCGA	9 cut(s)	18, 25, 265, 407, 714, 920, 949, 1133, 1262
TasI	AATT	8 cut(s)	50, 64, 218, 492, 624, 1031, 1092, 1201
TatI	WGTACW	2 cut(s)	244, 971
TauI	GCSGC	1 cut(s)	901
TfiI	GAWTC	5 cut(s)	404, 824, 1130, 1393, 1489
Tru1I	TTAA	9 cut(s)	201, 293, 482, 627, 1007, 1056, 1161, 1178, 1436
Tru9I	TTAA	9 cut(s)	201, 293, 482, 627, 1007, 1056, 1161, 1178, 1436
TscAI	CASTG	4 cut(s)	127, 896, 940, 994
TseFI	GTSAC	1 cut(s)	1350
TseI	GCWGC	5 cut(s)	449, 514, 517, 572, 1340
Tsp45I	GTSAC	1 cut(s)	1350
TspRI	CASTG	4 cut(s)	127, 896, 940, 994
Vha464I	CTTAAG	1 cut(s)	200
VpaK11BI	GGWCC	1 cut(s)	636
VspI	ATTAAT	1 cut(s)	482
XapI	RAATTY	3 cut(s)	64, 1031, 1201
XceI	RCATGY	2 cut(s)	914, 1347
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1362
XhoI	CTCGAG	2 cut(s)	24, 948
XmiI	GTMKAC	2 cut(s)	5, 1150
XspI	CTAG	2 cut(s)	210, 1104

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.