Rroxscaffold_3G00229780

WD-40 repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0008538
Physical Location & Seq
Reverse (-)
14334263 .. 14346214
11952 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00229780.1

Sequence Viewer

Length: 1290 bp
ATGATTGAACTTCGAGAAGTAGAGACTGCTCGTTCTATTTTAAGAAACACTCAGGTAATGGGTGTGATGAAGCAAGAACAACCAGAAAGATATTTGCGACTGGAGCATCTCCATGCCAGAACTTATTTCGATCCAAATGAGGCTTATCAAGATTCGACAAAAGAGAAACGGCGTGCACAAATTGCTCAAGCACTTGCTGCAGAAGTTACTGTGGTGCCACCTTCAAGATTAATGGCTCTAATCGGTCAGGCTCTAAAGTGGCAGCAGCACCAAGGATTACTTCCTCCAGGAACACAGTTCGACTTATTTAGGGGAACGGCAGCCATGAAACAGGATGTGGATGACTTGTATCCAACAACTCTTCAACACACAATTAAGTTTGGGACCAAAAGTCATGCAGAATGTGCTCGATTCTCACCAGATGGCCAGTTTCTTGTCTCTACATCTTTTGATGGATTTATCGAGGTATGGGATTACATAAGTGGGAAACTCAAGAAGGATCTTCAATACCAGGCTGATGAAGCTTTCATGCTCCATGACGATCCAGTCCTGTGTGCTGATTTTAGCAGAGATTCAGAGATGCTTGCATCTGGGGATCAATGTGGCAAGATCAAAGTTTGGCGTATTAGGACTGGTCAGTGCTTGCGGCGTCTTGAACATGCACATTCAAAGGGTGTCACCAGTGTTACCTTCTCTCGAGATGGAAGCCAAATACTTAGTACATCATTTGACAGCACTGCAAGGATCCATGGCTTAAAATCTGGGAAGCTGTTGAAAGAATTTCGCGGTCATTCATCTTACGTTAATAATGCAATCTTTACAAGTGATGGTGCCCGCGTAATTACCGCATCTAGTGATTGCACAGTCAAGGTCTGGGATTCGAAGACTGCCGACTGTCTACAGACATTTAAACCACCTCCTCCTTTAAGGGGAGGTGATGCTTCGGTGAATTCTGTTCATCTTTTCCCAAAAAGCAATGATCACATCGTTGTATGCAATAAGACTTCATCAATATATATCATGACACTTCAAGGACAGGTTGTGAAGAGCTTTTCATCTGGTAAAAGAGAAGGTGGAGATTTTATGGCTGCATGTGTGTCACCAAAAGGGGAATGGATTTACTGTGTTGGTGAAGACAGGAATCTGTATTGTTTCAGTTACCAATCTGGAAAGTTAGAGCATTTAATGAAGATCCATGAAAAGGATGTTATAGGTCTCGCTCATCATCCTCACAGGAATCTTCTGGCCACATTTGGTGAAGACTGCACAATGAAGTTATGGAAGCCTTGA

Protein Analysis

429

Amino Acids

48.2

Weight (kDa)

8.39

Isoelectric Point (pI)

39.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR3_2nd PF25172 119 - 241 8.7e-11 WDR3 second beta-propeller domain
Beta-prop_THOC3 PF25174 122 - 221 4e-12 THOC3 beta-propeller domain
WD40_Prp19 PF24814 128 - 235 5.1e-13 Prp19 WD40 domain
Beta-prop_EML_2 PF23414 129 - 214 5.2e-09 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR5 PF25175 130 - 428 1.1e-52 WDR5 beta-propeller domain
WDR55 PF24796 131 - 219 1.1e-08 WDR55
WD40_Gbeta PF25391 132 - 256 4e-07 G protein beta WD-40 repeat protein
Beta-prop_EML PF23409 136 - 242 2.3e-08 Echinoderm microtubule-associated protein first beta-propeller
WDR90_beta-prop_4th PF23342 138 - 261 1.8e-06 WDR90, 4th beta-propeller
Beta-prop_CAF1B_HIR1 PF24105 146 - 302 2.7e-10 CAF1B/HIR1 beta-propeller domain
Beta-prop_TEP1_2nd PF25047 173 - 296 1.3e-13 TEP-1 second beta-propeller
Beta-prop_WDR3_1st PF25173 179 - 303 1.6e-24 WDR3 first beta-propeller domain
Beta-prop_WDR36-Utp21_2nd PF25168 179 - 261 5.5e-07 WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR36-Utp21_1st PF25171 180 - 244 6.8e-06 WDR36/Utp21 first beta-propeller
WD40_CDC20-Fz PF24807 180 - 304 1e-13 CDC20/Fizzy WD40 domain
Beta-prop_ACSF4 PF13570 189 - 398 1.4e-07 Beta-alanine-activating enzyme, beta-propeller
WD40 PF00400 212 - 249 2.6e-06 WD domain, G-beta repeat
EIF3I PF24805 220 - 427 7.1e-16 EIF3I
WDR55 PF24796 220 - 304 2.2e-10 WDR55
WD40_Prp19 PF24814 220 - 353 7.2e-15 Prp19 WD40 domain
Beta-prop_THOC3 PF25174 222 - 427 3.2e-28 THOC3 beta-propeller domain
WD40_Gbeta PF25391 224 - 330 2.1e-08 G protein beta WD-40 repeat protein
WD40_WDHD1_1st PF24817 225 - 417 4.4e-17 WDHD1 first WD40 domain
Beta-prop_WDR3_2nd PF25172 226 - 304 6.3e-09 WDR3 second beta-propeller domain
WD40 PF00400 255 - 293 1.3e-08 WD domain, G-beta repeat
Beta-prop_WDR36-Utp21_2nd PF25168 257 - 306 8.4e-08 WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR36-Utp21_1st PF25171 279 - 427 9.7e-06 WDR36/Utp21 first beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

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Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 545
AccB1I GGYRCC 2 cut(s) 214, 830
AccI GTMKAC 1 cut(s) 898
AccII CGCG 2 cut(s) 786, 837
AciI CCGC 4 cut(s) 646, 786, 835, 846
AclWI GGATC 7 cut(s) 125, 507, 536, 603, 739, 752, 1186
AcoI YGGCCR 2 cut(s) 424, 1245
AcsI RAATTY 2 cut(s) 779, 949
AcyI GRCGYC 1 cut(s) 649
AfaI GTAC 1 cut(s) 721
AfiI CCNNNNNNNGG 2 cut(s) 929, 1201
AgsI TTSAA 8 cut(s) 8, 225, 365, 506, 656, 669, 775, 1031
AhdI GACNNNNNGTC 1 cut(s) 390
AjnI CCWGG 2 cut(s) 286, 510
AjuI GAANNNNNNNTTGG 2 cut(s) 264, 296
AluBI AGCT 3 cut(s) 524, 769, 1050
AluI AGCT 3 cut(s) 524, 769, 1050
Alw21I GWGCWC 2 cut(s) 178, 409
Alw26I GTCTC 3 cut(s) 17, 442, 1220
Alw44I GTGCAC 1 cut(s) 174
AlwI GGATC 7 cut(s) 125, 507, 536, 603, 739, 752, 1186
Ama87I CYCGRG 1 cut(s) 696
AoxI GGCC 2 cut(s) 424, 1245
ApaLI GTGCAC 1 cut(s) 174
ApeKI GCWGC 5 cut(s) 197, 262, 265, 320, 1088
ApoI RAATTY 2 cut(s) 779, 949
AseI ATTAAT 1 cut(s) 230
AspS9I GGNCC 1 cut(s) 384
AsuHPI GGTGA 7 cut(s) 408, 670, 947, 958, 1092, 1142, 1268
AsuII TTCGAA 1 cut(s) 881
AvaI CYCGRG 1 cut(s) 696
AvaII GGWCC 1 cut(s) 384
BaeGI GKGCMC 2 cut(s) 178, 835
BalI TGGCCA 2 cut(s) 426, 1247
BamHI GGATCC 1 cut(s) 744
BanI GGYRCC 2 cut(s) 214, 830
BbsI GAAGAC 3 cut(s) 890, 1140, 1266
Bbv12I GWGCWC 2 cut(s) 178, 409
BbvI GCAGC 5 cut(s) 184, 274, 277, 332, 1075
BccI CCATC 4 cut(s) 416, 446, 695, 821
BceAI ACGGC 2 cut(s) 185, 333
BciT130I CCWGG 2 cut(s) 288, 512
BciVI GTATCC 1 cut(s) 360
BclI TGATCA 1 cut(s) 979
BcoDI GTCTC 3 cut(s) 17, 442, 1220
BfaI CTAG 1 cut(s) 852
BfmI CTRYAG 2 cut(s) 198, 899
BfuI GTATCC 1 cut(s) 360
BisI GCNGC 6 cut(s) 198, 263, 266, 321, 647, 1089
BlsI GCNGC 6 cut(s) 199, 264, 267, 322, 648, 1090
Bme1390I CCNGG 2 cut(s) 288, 512
Bme18I GGWCC 1 cut(s) 384
BmeRI GACNNNNNGTC 1 cut(s) 390
BmeT110I CYCGRG 1 cut(s) 696
BmgT120I GGNCC 1 cut(s) 384
BmiI GGNNCC 4 cut(s) 216, 385, 746, 832
BmrFI CCNGG 2 cut(s) 288, 512
BmsI GCATC 5 cut(s) 115, 570, 596, 857, 928
BpiI GAAGAC 3 cut(s) 890, 1140, 1266
BpmI CTGGAG 2 cut(s) 122, 270
Bpu14I TTCGAA 1 cut(s) 881
BpuEI CTTGAG 2 cut(s) 171, 476
BsaHI GRCGYC 1 cut(s) 649
BsaI GGTCTC 1 cut(s) 1220
BsaJI CCNNGG 2 cut(s) 271, 748
Bsc4I CCNNNNNNNGG 2 cut(s) 929, 1201
Bse1I ACTGG 5 cut(s) 105, 427, 545, 637, 681
Bse3DI GCAATG 1 cut(s) 982
BseBI CCWGG 2 cut(s) 288, 512
BseDI CCNNGG 2 cut(s) 271, 748
BseGI GGATG 4 cut(s) 340, 346, 1210, 1225
BseLI CCNNNNNNNGG 2 cut(s) 929, 1201
BseMI GCAATG 1 cut(s) 982
BseMII CTCAG 1 cut(s) 65
BseNI ACTGG 5 cut(s) 105, 427, 545, 637, 681
BseRI GAGGAG 1 cut(s) 909
BseSI GKGCMC 2 cut(s) 178, 835
BseXI GCAGC 5 cut(s) 184, 274, 277, 332, 1075
BsgI GTGCAG 1 cut(s) 1249
Bsh1236I CGCG 2 cut(s) 786, 837
BshFI GGCC 2 cut(s) 426, 1247
BshNI GGYRCC 2 cut(s) 214, 830
BsiHKAI GWGCWC 2 cut(s) 178, 409
BsiHKCI CYCGRG 1 cut(s) 696
BslFI GGGAC 1 cut(s) 397
BslI CCNNNNNNNGG 2 cut(s) 929, 1201
BsmAI GTCTC 3 cut(s) 17, 442, 1220
BsmFI GGGAC 1 cut(s) 397
BsnI GGCC 2 cut(s) 426, 1247
Bso31I GGTCTC 1 cut(s) 1220
BsoBI CYCGRG 1 cut(s) 696
Bsp119I TTCGAA 1 cut(s) 881
Bsp1286I GDGCHC 3 cut(s) 178, 409, 835
Bsp143I GATC 8 cut(s) 130, 499, 541, 595, 609, 744, 979, 1191
Bsp19I CCATGG 1 cut(s) 748
BspACI CCGC 4 cut(s) 646, 786, 835, 846
BspANI GGCC 2 cut(s) 426, 1247
BspCNI CTCAG 1 cut(s) 64
BspFNI CGCG 2 cut(s) 786, 837
BspHI TCATGA 1 cut(s) 1020
BspLI GGNNCC 4 cut(s) 216, 385, 746, 832
BspMAI CTGCAG 1 cut(s) 202
BspPI GGATC 7 cut(s) 125, 507, 536, 603, 739, 752, 1186
BspQI GCTCTTC 1 cut(s) 1040
BspT104I TTCGAA 1 cut(s) 881
BspT107I GGYRCC 2 cut(s) 214, 830
BspTNI GGTCTC 1 cut(s) 1220
BsrDI GCAATG 1 cut(s) 982
BsrI ACTGG 5 cut(s) 105, 427, 545, 637, 681
BssECI CCNNGG 2 cut(s) 271, 748
BssMI GATC 8 cut(s) 130, 499, 541, 595, 609, 744, 979, 1191
BssNI GRCGYC 1 cut(s) 649
BssT1I CCWWGG 2 cut(s) 271, 748
Bst2UI CCWGG 2 cut(s) 288, 512
Bst4CI ACNGT 5 cut(s) 211, 297, 865, 896, 1124
Bst6I CTCTTC 2 cut(s) 366, 1040
BstACI GRCGYC 1 cut(s) 649
BstAPI GCANNNNNTGC 3 cut(s) 182, 197, 404
BstBI TTCGAA 1 cut(s) 881
BstC8I GCNNGC 4 cut(s) 174, 585, 644, 835
BstDEI CTNAG 2 cut(s) 51, 716
BstDSI CCRYGG 1 cut(s) 748
BstF5I GGATG 4 cut(s) 340, 346, 1210, 1225
BstFNI CGCG 2 cut(s) 786, 837
BstKTI GATC 8 cut(s) 133, 502, 544, 598, 612, 747, 982, 1194
BstMAI GTCTC 3 cut(s) 17, 442, 1220
BstMBI GATC 8 cut(s) 130, 499, 541, 595, 609, 744, 979, 1191
BstMWI GCNNNNNNNGC 5 cut(s) 103, 182, 197, 404, 521
BstNI CCWGG 2 cut(s) 288, 512
BstNSI RCATGY 2 cut(s) 662, 1095
BstSCI CCNGG 2 cut(s) 286, 510
BstSFI CTRYAG 2 cut(s) 198, 899
BstSLI GKGCMC 2 cut(s) 178, 835
BstUI CGCG 2 cut(s) 786, 837
BstV1I GCAGC 5 cut(s) 184, 274, 277, 332, 1075
BstV2I GAAGAC 3 cut(s) 890, 1140, 1266
BstX2I RGATCY 3 cut(s) 499, 744, 1191
BstYI RGATCY 3 cut(s) 499, 744, 1191
BsuI GTATCC 1 cut(s) 360
BsuRI GGCC 2 cut(s) 426, 1247
BtgI CCRYGG 1 cut(s) 748
BtsCI GGATG 4 cut(s) 340, 346, 1210, 1225
BtsI GCAGTG 1 cut(s) 735
BtsIMutI CAGTG 3 cut(s) 644, 688, 735
Cac8I GCNNGC 4 cut(s) 174, 585, 644, 835
CciI TCATGA 1 cut(s) 1020
Cfr13I GGNCC 1 cut(s) 384
CseI GACGC 1 cut(s) 638
Csp6I GTAC 1 cut(s) 720
CviQI GTAC 1 cut(s) 720
DdeI CTNAG 2 cut(s) 51, 716
DpnI GATC 8 cut(s) 132, 501, 543, 597, 611, 746, 981, 1193
DpnII GATC 8 cut(s) 130, 499, 541, 595, 609, 744, 979, 1191
DraI TTTAAA 1 cut(s) 910
DrdI GACNNNNNNGTC 1 cut(s) 545
DriI GACNNNNNGTC 1 cut(s) 390
DseDI GACNNNNNNGTC 1 cut(s) 545
EaeI YGGCCR 2 cut(s) 424, 1245
Eam1104I CTCTTC 2 cut(s) 366, 1040
Eam1105I GACNNNNNGTC 1 cut(s) 390
EarI CTCTTC 2 cut(s) 366, 1040
Eco130I CCWWGG 2 cut(s) 271, 748
Eco31I GGTCTC 1 cut(s) 1220
Eco47I GGWCC 1 cut(s) 384
Eco88I CYCGRG 1 cut(s) 696
EcoRI GAATTC 1 cut(s) 949
EcoRII CCWGG 2 cut(s) 286, 510
EcoT14I CCWWGG 2 cut(s) 271, 748
ErhI CCWWGG 2 cut(s) 271, 748
FalI AAGNNNNNCTT 2 cut(s) 264, 296
FaqI GGGAC 1 cut(s) 397
FauI CCCGC 1 cut(s) 842
FbaI TGATCA 1 cut(s) 979
FblI GTMKAC 1 cut(s) 898
Fnu4HI GCNGC 6 cut(s) 198, 263, 266, 321, 647, 1089
FokI GGATG 4 cut(s) 347, 353, 1212, 1217
Fsp4HI GCNGC 6 cut(s) 198, 263, 266, 321, 647, 1089
FspBI CTAG 1 cut(s) 852
GluI GCNGC 6 cut(s) 198, 263, 266, 321, 647, 1089
GsuI CTGGAG 2 cut(s) 122, 270
HaeIII GGCC 2 cut(s) 426, 1247
HgaI GACGC 1 cut(s) 638
Hin1I GRCGYC 1 cut(s) 649
HindIII AAGCTT 1 cut(s) 522
HinfI GANTC 6 cut(s) 152, 411, 572, 878, 1141, 1237
HphI GGTGA 7 cut(s) 408, 670, 947, 958, 1092, 1142, 1268
Hpy166II GTNNAC 2 cut(s) 176, 899
Hpy188I TCNGA 1 cut(s) 577
Hpy188III TCNNGA 9 cut(s) 14, 149, 225, 493, 653, 696, 698, 1021, 1167
Hpy8I GTNNAC 2 cut(s) 176, 899
HpyAV CCTTC 4 cut(s) 231, 490, 700, 1064
HpyCH4III ACNGT 5 cut(s) 211, 297, 865, 896, 1124
HpyCH4IV ACGT 1 cut(s) 801
HpyF10VI GCNNNNNNNGC 5 cut(s) 103, 182, 197, 404, 521
HpyF3I CTNAG 2 cut(s) 51, 716
HpySE526I ACGT 1 cut(s) 801
Hsp92I GRCGYC 1 cut(s) 649
Ksp22I TGATCA 1 cut(s) 979
Kzo9I GATC 8 cut(s) 130, 499, 541, 595, 609, 744, 979, 1191
LguI GCTCTTC 1 cut(s) 1040
LmnI GCTCC 2 cut(s) 103, 537
Lsp1109I GCAGC 5 cut(s) 184, 274, 277, 332, 1075
LweI GCATC 5 cut(s) 115, 570, 596, 857, 928
MaeI CTAG 1 cut(s) 852
MaeII ACGT 1 cut(s) 801
MaeIII GTNAC 5 cut(s) 205, 676, 685, 1098, 1157
MalI GATC 8 cut(s) 132, 501, 543, 597, 611, 746, 981, 1193
MboI GATC 8 cut(s) 130, 499, 541, 595, 609, 744, 979, 1191
MboII GAAGA 8 cut(s) 353, 494, 895, 1057, 1145, 1201, 1232, 1271
MflI RGATCY 3 cut(s) 499, 744, 1191
MhlI GDGCHC 3 cut(s) 178, 409, 835
MlsI TGGCCA 2 cut(s) 426, 1247
MluCI AATT 5 cut(s) 180, 372, 779, 840, 949
MluNI TGGCCA 2 cut(s) 426, 1247
MmeI TCCRAC 1 cut(s) 377
MnlI CCTC 7 cut(s) 133, 294, 457, 926, 927, 930, 1239
Mox20I TGGCCA 2 cut(s) 426, 1247
MscI TGGCCA 2 cut(s) 426, 1247
MseI TTAA 8 cut(s) 41, 230, 375, 755, 804, 909, 926, 1184
MslI CAYNNNNRTG 1 cut(s) 111
Msp20I TGGCCA 2 cut(s) 426, 1247
MspR9I CCNGG 2 cut(s) 288, 512
MvaI CCWGG 2 cut(s) 288, 512
MvnI CGCG 2 cut(s) 786, 837
MwoI GCNNNNNNNGC 5 cut(s) 103, 182, 197, 404, 521
NcoI CCATGG 1 cut(s) 748
NdeII GATC 8 cut(s) 130, 499, 541, 595, 609, 744, 979, 1191
NlaIV GGNNCC 4 cut(s) 216, 385, 746, 832
NmuCI GTSAC 2 cut(s) 676, 1098
NspI RCATGY 2 cut(s) 662, 1095
NspV TTCGAA 1 cut(s) 881
PaeR7I CTCGAG 1 cut(s) 696
PagI TCATGA 1 cut(s) 1020
PciSI GCTCTTC 1 cut(s) 1040
PfeI GAWTC 6 cut(s) 152, 411, 572, 878, 1141, 1237
PfoI TCCNGGA 1 cut(s) 286
PkrI GCNGC 6 cut(s) 199, 264, 267, 322, 648, 1090
PshBI ATTAAT 1 cut(s) 230
Psp6I CCWGG 2 cut(s) 286, 510
PspGI CCWGG 2 cut(s) 286, 510
PspN4I GGNNCC 4 cut(s) 216, 385, 746, 832
PspPI GGNCC 1 cut(s) 384
PstI CTGCAG 1 cut(s) 202
PsuI RGATCY 3 cut(s) 499, 744, 1191
RsaI GTAC 1 cut(s) 721
RsaNI GTAC 1 cut(s) 720
RseI CAYNNNNRTG 1 cut(s) 111
SapI GCTCTTC 1 cut(s) 1040
SaqAI TTAA 8 cut(s) 41, 230, 375, 755, 804, 909, 926, 1184
SatI GCNGC 6 cut(s) 198, 263, 266, 321, 647, 1089
Sau3AI GATC 8 cut(s) 130, 499, 541, 595, 609, 744, 979, 1191
Sau96I GGNCC 1 cut(s) 384
ScrFI CCNGG 2 cut(s) 288, 512
SduI GDGCHC 3 cut(s) 178, 409, 835
SfaNI GCATC 5 cut(s) 115, 570, 596, 857, 928
SfcI CTRYAG 2 cut(s) 198, 899
Sfr274I CTCGAG 1 cut(s) 696
SfuI TTCGAA 1 cut(s) 881
SinI GGWCC 1 cut(s) 384
SlaI CTCGAG 1 cut(s) 696
SmiMI CAYNNNNRTG 1 cut(s) 111
SmlI CTYRAG 3 cut(s) 186, 491, 696
SmoI CTYRAG 3 cut(s) 186, 491, 696
Sse9I AATT 5 cut(s) 180, 372, 779, 840, 949
SsiI CCGC 4 cut(s) 646, 786, 835, 846
SspMI CTAG 1 cut(s) 852
StyD4I CCNGG 2 cut(s) 286, 510
StyI CCWWGG 2 cut(s) 271, 748
TaaI ACNGT 5 cut(s) 211, 297, 865, 896, 1124
TaiI ACGT 1 cut(s) 804
TaqI TCGA 8 cut(s) 13, 129, 155, 300, 409, 462, 697, 881
TaqII GACCGA 1 cut(s) 233
TasI AATT 5 cut(s) 180, 372, 779, 840, 949
TatI WGTACW 1 cut(s) 719
TauI GCSGC 1 cut(s) 649
TfiI GAWTC 6 cut(s) 152, 411, 572, 878, 1141, 1237
Tru1I TTAA 8 cut(s) 41, 230, 375, 755, 804, 909, 926, 1184
Tru9I TTAA 8 cut(s) 41, 230, 375, 755, 804, 909, 926, 1184
TscAI CASTG 3 cut(s) 644, 688, 742
TseFI GTSAC 2 cut(s) 676, 1098
TseI GCWGC 5 cut(s) 197, 262, 265, 320, 1088
Tsp45I GTSAC 2 cut(s) 676, 1098
TspRI CASTG 3 cut(s) 644, 688, 742
VneI GTGCAC 1 cut(s) 174
VpaK11BI GGWCC 1 cut(s) 384
VspI ATTAAT 1 cut(s) 230
XapI RAATTY 2 cut(s) 779, 949
XceI RCATGY 2 cut(s) 662, 1095
XcmI CCANNNNNNNNNTGG 1 cut(s) 1110
XhoI CTCGAG 1 cut(s) 696
XmiI GTMKAC 1 cut(s) 898
XspI CTAG 1 cut(s) 852
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.