AT1G74240

Belongs to the mitochondrial carrier (TC 2.A.29) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0010862
Physical Location & Seq
Forward (+)
27917249 .. 27920192
2944 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G74240.2

Sequence Viewer

Length: 1095 bp
ATGGCGACGAAGAGCTCGGATTCGAGCCATAAGCAATCTCCTCCGAGTTTTCGTAAGAGCGTCGAAATCAAAGCCACGCATGATCAATTTTTCGTTTGGAGAGAGTTCTTATGGGGAGGTATTGCTGGAGCTTTTGGAGAAGGAATGATGCATCCCGTTGATACTCTCAAAACTAGACTTCAAAGTCAAATTATCATGAATGCAACTCAGAGACAAAAGAGTATTCTACAAATGCTCCGAACTGTCTGGGTTGGTGATGGCTTAAAAGGCTTTTATAGGGGCATTGCTCCTGGAGTCACTGGATCTCTTGCCACTGGAGCAACTTACTTTGGTTTTATTGAGTCAACAAAGAAATGGATTGAAGAGTCTCATCCCAGCTTAGCAGGGCACTGGGCGCATTTTATAGCAGGAGCAGTTGGCGATACACTTGGTTCTTTCATATATGTTCCTTGTGAAGTCATAAAGCAGCGAATGCAAATTCAAGGCACTAGTAGCTCCTGGAGCTCTTATATTTCGAGGAACAGCGTTCCGGTGCAACCAAGAGGTGACATGTATGGTTATTACACTGGAATGTTCCAGGCTGGATGCTCAATATGGAAAGAGCAAGGGCCAAAAGGACTATATGCTGGATATTGGTCTACACTAGCGAGGGATGTGCCTTTTGCTGGTCTTATGGTAGTGTTTTATGAAGGATTAAAGGATCTAACAGATCAAGGAAAGAAAAAGTTTCCACAGTATGGAGTCAACAGTTCGATAGAGGGTCTCGTATTGGGAGGATTAGCTGGTGGACTAAGTGCTTATCTCACAACTCCACTGGATGTTGTAAAAACAAGACTGCAAGTGCAAGGATCAACGATCAAGTACAAGGGATGGTTGGATGCAGTTGGACAGATATGGAGGAAGGAAGGTCCACAAGGGTTTTTCCGAGGAAGCGTCCCAAGGGTCATGTGGTATCTTCCGGCCTCGGCACTCACCTTCATGGCCGTTGAGTTCCTTAGAGATAACTTCAGAGAGAAAAGTAATAATAATAACAATGTCGTCTCAAATTTGAGCATAGAGAGGAAAACATCTTCTACTTCAGTGCATGAAGGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF647 Mito_carr

Protein Analysis

364

Amino Acids

40.31

Weight (kDa)

9.55

Isoelectric Point (pI)

37.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mito_carr PF00153 2 - 74 5.9e-15 Mitochondrial carrier protein
Mito_carr PF00153 82 - 187 7.4e-18 Mitochondrial carrier protein
Mito_carr PF00153 202 - 291 8.8e-25 Mitochondrial carrier protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0010862)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G74240 AT1G74240
fragaria_vesca FvH4_5g13870 FvH4_5g13870 FvH4_5g13870
malus_domestica MD06G1202800.v1.1 MD14G1211900.v1.1
prunus_persica Prupe.5G204700_v2.0.a1
pyrus_communis pycom14g17550
rosa_chinensis RchiOBHm_Chr7g0183061
rosa_laevigata RLG00000005082
rosa_multiflora Rmu_sc0000014.1_g000003
rosa_roxburghii Rroxscaffold_3G00270800
rosa_rugosa Rorug06G0452200 Rorug06G0452300
rosa_samantha Rh7AG055700 Rh7BG055000 Rh7CG056400 Rh7DG055200
rosa_wichuraiana Rw7G004530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 183
AccB7I CCANNNNNTGG 1 cut(s) 737
AccI GTMKAC 1 cut(s) 638
AclWI GGATC 3 cut(s) 310, 708, 856
AcoI YGGCCR 1 cut(s) 981
AcsI RAATTY 2 cut(s) 477, 1045
AcuI CTGAAG 2 cut(s) 991, 1062
AfaI GTAC 1 cut(s) 863
AfiI CCNNNNNNNGG 2 cut(s) 665, 737
AflIII ACRYGT 1 cut(s) 549
AgsI TTSAA 3 cut(s) 182, 362, 482
AhlI ACTAGT 1 cut(s) 488
AjnI CCWGG 3 cut(s) 289, 497, 576
AluBI AGCT 6 cut(s) 15, 131, 378, 495, 504, 782
AluI AGCT 6 cut(s) 15, 131, 378, 495, 504, 782
Alw21I GWGCWC 2 cut(s) 17, 506
Alw26I GTCTC 4 cut(s) 205, 372, 767, 1045
AlwI GGATC 3 cut(s) 310, 708, 856
AoxI GGCC 3 cut(s) 608, 960, 981
ApeKI GCWGC 1 cut(s) 466
ApoI RAATTY 2 cut(s) 477, 1045
AspLEI GCGC 1 cut(s) 397
AspS9I GGNCC 2 cut(s) 608, 908
AsuHPI GGTGA 3 cut(s) 266, 557, 964
AvaII GGWCC 1 cut(s) 908
BaeGI GKGCMC 1 cut(s) 390
BanII GRGCYC 2 cut(s) 17, 506
Bbv12I GWGCWC 2 cut(s) 17, 506
BbvI GCAGC 1 cut(s) 478
BccI CCATC 2 cut(s) 251, 864
BceAI ACGGC 1 cut(s) 968
BcgI CGANNNNNNTGC 2 cut(s) 637, 671
BciT130I CCWGG 3 cut(s) 291, 499, 578
BclI TGATCA 1 cut(s) 82
BcoDI GTCTC 4 cut(s) 205, 372, 767, 1045
BcuI ACTAGT 1 cut(s) 488
BfaI CTAG 3 cut(s) 174, 489, 644
BisI GCNGC 1 cut(s) 467
BlpI GCTNAGC 1 cut(s) 379
BlsI GCNGC 1 cut(s) 468
Bme1390I CCNGG 3 cut(s) 291, 499, 578
Bme18I GGWCC 1 cut(s) 908
BmgT120I GGNCC 2 cut(s) 608, 908
BmrFI CCNGG 3 cut(s) 291, 499, 578
BmrI ACTGGG 1 cut(s) 400
BmsI GCATC 4 cut(s) 138, 160, 575, 868
BmuI ACTGGG 1 cut(s) 400
BpmI CTGGAG 4 cut(s) 147, 312, 336, 520
Bpu1102I GCTNAGC 1 cut(s) 379
BsaI GGTCTC 1 cut(s) 767
BsaJI CCNNGG 3 cut(s) 925, 938, 963
BsaWI WCCGGW 1 cut(s) 529
BsaXI ACNNNNNCTCC 2 cut(s) 219, 249
Bsc4I CCNNNNNNNGG 2 cut(s) 665, 737
Bse1I ACTGG 5 cut(s) 304, 319, 395, 571, 819
Bse3DI GCAATG 1 cut(s) 282
BseBI CCWGG 3 cut(s) 291, 499, 578
BseDI CCNNGG 3 cut(s) 925, 938, 963
BseGI GGATG 7 cut(s) 151, 370, 590, 658, 823, 875, 883
BseLI CCNNNNNNNGG 2 cut(s) 665, 737
BseMI GCAATG 1 cut(s) 282
BseMII CTCAG 1 cut(s) 221
BseNI ACTGG 5 cut(s) 304, 319, 395, 571, 819
BseRI GAGGAG 1 cut(s) 30
BseSI GKGCMC 1 cut(s) 390
BseXI GCAGC 1 cut(s) 478
BseYI CCCAGC 1 cut(s) 374
BshFI GGCC 3 cut(s) 610, 962, 983
BsiHKAI GWGCWC 2 cut(s) 17, 506
BsiSI CCGG 2 cut(s) 530, 959
BslFI GGGAC 1 cut(s) 920
BslI CCNNNNNNNGG 2 cut(s) 665, 737
BsmAI GTCTC 4 cut(s) 205, 372, 767, 1045
BsmBI CGTCTC 1 cut(s) 1045
BsmFI GGGAC 1 cut(s) 920
BsmI GAATGC 2 cut(s) 205, 477
BsnI GGCC 3 cut(s) 610, 962, 983
Bso31I GGTCTC 1 cut(s) 767
Bsp1286I GDGCHC 3 cut(s) 17, 390, 506
Bsp143I GATC 6 cut(s) 82, 302, 700, 709, 848, 855
Bsp1720I GCTNAGC 1 cut(s) 379
BspANI GGCC 3 cut(s) 610, 962, 983
BspCNI CTCAG 1 cut(s) 220
BspHI TCATGA 1 cut(s) 195
BspPI GGATC 3 cut(s) 310, 708, 856
BspQI GCTCTTC 1 cut(s) 5
BspTNI GGTCTC 1 cut(s) 767
BsrDI GCAATG 1 cut(s) 282
BsrI ACTGG 5 cut(s) 304, 319, 395, 571, 819
BssECI CCNNGG 3 cut(s) 925, 938, 963
BssMI GATC 6 cut(s) 82, 302, 700, 709, 848, 855
BssT1I CCWWGG 1 cut(s) 938
Bst2UI CCWGG 3 cut(s) 291, 499, 578
Bst4CI ACNGT 3 cut(s) 244, 735, 749
Bst6I CTCTTC 2 cut(s) 5, 357
BstAPI GCANNNNNTGC 1 cut(s) 472
BstDEI CTNAG 4 cut(s) 207, 379, 791, 995
BstF5I GGATG 7 cut(s) 151, 370, 590, 658, 823, 875, 883
BstHHI GCGC 1 cut(s) 397
BstKTI GATC 6 cut(s) 85, 305, 703, 712, 851, 858
BstMAI GTCTC 4 cut(s) 205, 372, 767, 1045
BstMBI GATC 6 cut(s) 82, 302, 700, 709, 848, 855
BstMWI GCNNNNNNNGC 6 cut(s) 267, 317, 394, 472, 492, 501
BstNI CCWGG 3 cut(s) 291, 499, 578
BstNSI RCATGY 1 cut(s) 553
BstSCI CCNGG 3 cut(s) 289, 497, 576
BstSLI GKGCMC 1 cut(s) 390
BstV1I GCAGC 1 cut(s) 478
BstX2I RGATCY 2 cut(s) 302, 700
BstYI RGATCY 2 cut(s) 302, 700
BsuRI GGCC 3 cut(s) 610, 962, 983
BtsCI GGATG 7 cut(s) 151, 370, 590, 658, 823, 875, 883
BtsIMutI CAGTG 6 cut(s) 297, 312, 388, 564, 812, 1086
CciI TCATGA 1 cut(s) 195
CfoI GCGC 1 cut(s) 397
Cfr13I GGNCC 2 cut(s) 608, 908
CseI GACGC 2 cut(s) 49, 922
Csp6I GTAC 1 cut(s) 862
CviAII CATG 6 cut(s) 80, 196, 550, 946, 979, 1085
CviQI GTAC 1 cut(s) 862
DdeI CTNAG 4 cut(s) 207, 379, 791, 995
DpnI GATC 6 cut(s) 84, 304, 702, 711, 850, 857
DpnII GATC 6 cut(s) 82, 302, 700, 709, 848, 855
DrdI GACNNNNNNGTC 1 cut(s) 183
DseDI GACNNNNNNGTC 1 cut(s) 183
EaeI YGGCCR 1 cut(s) 981
Eam1104I CTCTTC 2 cut(s) 5, 357
EarI CTCTTC 2 cut(s) 5, 357
Ecl136II GAGCTC 2 cut(s) 15, 504
Eco130I CCWWGG 1 cut(s) 938
Eco24I GRGCYC 2 cut(s) 17, 506
Eco31I GGTCTC 1 cut(s) 767
Eco47I GGWCC 1 cut(s) 908
Eco53kI GAGCTC 2 cut(s) 15, 504
Eco57I CTGAAG 2 cut(s) 991, 1062
EcoICRI GAGCTC 2 cut(s) 15, 504
EcoRII CCWGG 3 cut(s) 289, 497, 576
EcoT14I CCWWGG 1 cut(s) 938
EcoT22I ATGCAT 1 cut(s) 153
EcoT38I GRGCYC 2 cut(s) 17, 506
ErhI CCWWGG 1 cut(s) 938
Esp3I CGTCTC 1 cut(s) 1045
FaeI CATG 6 cut(s) 83, 199, 553, 949, 982, 1088
FaqI GGGAC 1 cut(s) 920
FatI CATG 6 cut(s) 79, 195, 549, 945, 978, 1084
FbaI TGATCA 1 cut(s) 82
FblI GTMKAC 1 cut(s) 638
Fnu4HI GCNGC 1 cut(s) 467
FokI GGATG 7 cut(s) 138, 357, 597, 665, 830, 882, 890
FriOI GRGCYC 2 cut(s) 17, 506
Fsp4HI GCNGC 1 cut(s) 467
FspBI CTAG 3 cut(s) 174, 489, 644
GlaI GCGC 1 cut(s) 396
GluI GCNGC 1 cut(s) 467
GsaI CCCAGC 1 cut(s) 378
GsuI CTGGAG 4 cut(s) 147, 312, 336, 520
HaeIII GGCC 3 cut(s) 610, 962, 983
HapII CCGG 2 cut(s) 530, 959
HgaI GACGC 2 cut(s) 49, 922
HhaI GCGC 1 cut(s) 397
Hin1II CATG 6 cut(s) 83, 199, 553, 949, 982, 1088
Hin6I GCGC 1 cut(s) 395
HinP1I GCGC 1 cut(s) 395
HincII GTYRAC 2 cut(s) 345, 745
HindII GTYRAC 2 cut(s) 345, 745
HinfI GANTC 5 cut(s) 20, 294, 341, 365, 741
HpaII CCGG 2 cut(s) 530, 959
HphI GGTGA 3 cut(s) 266, 557, 964
Hpy166II GTNNAC 5 cut(s) 345, 639, 745, 788, 911
Hpy188I TCNGA 6 cut(s) 19, 45, 210, 239, 926, 1010
Hpy188III TCNNGA 1 cut(s) 196
Hpy8I GTNNAC 5 cut(s) 345, 639, 745, 788, 911
Hpy99I CGWCG 2 cut(s) 10, 65
HpyAV CCTTC 6 cut(s) 134, 683, 895, 899, 985, 1082
HpyCH4III ACNGT 3 cut(s) 244, 735, 749
HpyCH4V TGCA 8 cut(s) 151, 203, 475, 535, 838, 844, 881, 1084
HpyF10VI GCNNNNNNNGC 6 cut(s) 267, 317, 394, 472, 492, 501
HpyF3I CTNAG 4 cut(s) 207, 379, 791, 995
Hsp92II CATG 6 cut(s) 83, 199, 553, 949, 982, 1088
HspAI GCGC 1 cut(s) 395
Ksp22I TGATCA 1 cut(s) 82
Kzo9I GATC 6 cut(s) 82, 302, 700, 709, 848, 855
LguI GCTCTTC 1 cut(s) 5
LmnI GCTCC 7 cut(s) 128, 240, 292, 317, 410, 500, 501
Lsp1109I GCAGC 1 cut(s) 478
LweI GCATC 4 cut(s) 138, 160, 575, 868
MaeI CTAG 3 cut(s) 174, 489, 644
MaeIII GTNAC 2 cut(s) 295, 545
MalI GATC 6 cut(s) 84, 304, 702, 711, 850, 857
MboI GATC 6 cut(s) 82, 302, 700, 709, 848, 855
MboII GAAGA 4 cut(s) 22, 374, 947, 1062
MflI RGATCY 2 cut(s) 302, 700
MhlI GDGCHC 3 cut(s) 17, 390, 506
MluCI AATT 4 cut(s) 86, 189, 477, 1045
MlyI GAGTC 4 cut(s) 303, 350, 374, 750
MmeI TCCRAC 2 cut(s) 855, 865
Mph1103I ATGCAT 1 cut(s) 153
MseI TTAA 2 cut(s) 263, 695
MslI CAYNNNNRTG 2 cut(s) 569, 977
MspI CCGG 2 cut(s) 530, 959
MspR9I CCNGG 3 cut(s) 291, 499, 578
Mva1269I GAATGC 2 cut(s) 205, 477
MvaI CCWGG 3 cut(s) 291, 499, 578
MwoI GCNNNNNNNGC 6 cut(s) 267, 317, 394, 472, 492, 501
NdeII GATC 6 cut(s) 82, 302, 700, 709, 848, 855
NlaIII CATG 6 cut(s) 83, 199, 553, 949, 982, 1088
NmeAIII GCCGAG 1 cut(s) 944
NmuCI GTSAC 2 cut(s) 295, 545
NsiI ATGCAT 1 cut(s) 153
NspI RCATGY 1 cut(s) 553
PagI TCATGA 1 cut(s) 195
PciI ACATGT 1 cut(s) 549
PciSI GCTCTTC 1 cut(s) 5
PctI GAATGC 2 cut(s) 205, 477
PfeI GAWTC 1 cut(s) 20
PflMI CCANNNNNTGG 1 cut(s) 737
PfoI TCCNGGA 2 cut(s) 289, 497
PkrI GCNGC 1 cut(s) 468
PleI GAGTC 4 cut(s) 302, 349, 373, 749
PpsI GAGTC 4 cut(s) 302, 349, 373, 749
PscI ACATGT 1 cut(s) 549
Psp124BI GAGCTC 2 cut(s) 17, 506
Psp6I CCWGG 3 cut(s) 289, 497, 576
PspFI CCCAGC 1 cut(s) 374
PspGI CCWGG 3 cut(s) 289, 497, 576
PspPI GGNCC 2 cut(s) 608, 908
PsuI RGATCY 2 cut(s) 302, 700
RsaI GTAC 1 cut(s) 863
RsaNI GTAC 1 cut(s) 862
RseI CAYNNNNRTG 2 cut(s) 569, 977
SacI GAGCTC 2 cut(s) 17, 506
SapI GCTCTTC 1 cut(s) 5
SaqAI TTAA 2 cut(s) 263, 695
SatI GCNGC 1 cut(s) 467
Sau3AI GATC 6 cut(s) 82, 302, 700, 709, 848, 855
Sau96I GGNCC 2 cut(s) 608, 908
SchI GAGTC 4 cut(s) 303, 350, 374, 750
ScrFI CCNGG 3 cut(s) 291, 499, 578
SduI GDGCHC 3 cut(s) 17, 390, 506
SfaNI GCATC 4 cut(s) 138, 160, 575, 868
SinI GGWCC 1 cut(s) 908
SmiMI CAYNNNNRTG 2 cut(s) 569, 977
SpeI ACTAGT 1 cut(s) 488
Sse9I AATT 4 cut(s) 86, 189, 477, 1045
SspMI CTAG 3 cut(s) 174, 489, 644
SstI GAGCTC 2 cut(s) 17, 506
StyD4I CCNGG 3 cut(s) 289, 497, 576
StyI CCWWGG 1 cut(s) 938
TaaI ACNGT 3 cut(s) 244, 735, 749
TaqI TCGA 4 cut(s) 23, 63, 515, 752
TasI AATT 4 cut(s) 86, 189, 477, 1045
TatI WGTACW 1 cut(s) 861
TfiI GAWTC 1 cut(s) 20
Tru1I TTAA 2 cut(s) 263, 695
Tru9I TTAA 2 cut(s) 263, 695
TscAI CASTG 6 cut(s) 304, 319, 395, 571, 819, 1086
TseFI GTSAC 2 cut(s) 295, 545
TseI GCWGC 1 cut(s) 466
Tsp45I GTSAC 2 cut(s) 295, 545
TspDTI ATGAA 4 cut(s) 212, 427, 702, 967
TspRI CASTG 6 cut(s) 304, 319, 395, 571, 819, 1086
Van91I CCANNNNNTGG 1 cut(s) 737
VpaK11BI GGWCC 1 cut(s) 908
XapI RAATTY 2 cut(s) 477, 1045
XceI RCATGY 1 cut(s) 553
XcmI CCANNNNNNNNNTGG 1 cut(s) 945
XmiI GTMKAC 1 cut(s) 638
XspI CTAG 3 cut(s) 174, 489, 644
Zsp2I ATGCAT 1 cut(s) 153
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.