MD14G1211900.v1.1

Belongs to the mitochondrial carrier (TC 2.A.29) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0010862
Physical Location & Seq
Forward (+)
29699429 .. 29703242
3814 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1211900.v1.1.491

Sequence Viewer

Length: 1080 bp
ATGGCGACACAGAGCTCGAATTCGAGCGAACGGCATGCCCTATCGGATACGAATCACAAACACTTCTTCGTTATGAGGGAGTTTCTGTGGGGAGCGATTGCTGGAGCATTTGGGGAAGGAATGATGCATCCGGTCGACACCGTAAAAACACGCATTCAGAGCCAGGCAATTCTCTCTGCAAGTCAGAAACCGAAGACCATACTTCAGATGGTACGAGCGGTCTGGGTAGCTGATGGATTAAGAGGCTTTTATAGGGGTATAGCTCCGGGTGTCACTGGTTCTCTTGCAACTGGAGCAACATATTTTGGTGTCATAGAGTCGACCAAAAAGTGGATTGAAGAATCACATCCAAATCTTGAGGGACATTGGGCACATTTCATTGCTGGAGCTGTTGGAGATACACTTGGTTCTGTTGTATATGTTCCATGTGAAGTGATGAAGCAACGCATGCAGGTTCAAGGCACGATGGCCTCTTGGAGTTCTGTTGTCATGAAAGATAATATCGCTATGAAGCCTGGTAGCCAAATGTATGGTTACTATACTGGGATGTTCCATGCTGGCTGCTCAATATGGAAGGAGCAGGGGCTAAGAGGATTATATGCAGGATACTGGTCCACACTGGCAAGGGACGTGCCTTTTGCTGGTTTAATGGTTATGTTTTACGAAGGCTTGAAAGATTTGACAGAGCATGGGAAGCAAAAATACATACGGGATCCAAATTTCCATTTTAATAGTTCTGTCGAGGGGCTTGTACTAGGAGGATTGGCTGGTGGCATTAGTGCGTATCTTACCACTCCCTTGGATGTGATCAAAACACGACTGCAAGTACAAGGATCAACCTTGAGGTACAAAGGCTGGTTGGATGCAATTCACAACGTATGGAGGGCCGAAGGTGCAAAAGGGATGTTCAGAGGTAGCGTCCCCAGGATTACATGGTATGTTCCAGCTTCAGCACTTACGTTTATGGCCGTGGAATTTCTCAGAGAACATTTCGACGAAGGATTGAGCAACGATGACACTCGAGAAGTTGCTAGCGTGTCAGTGGAGAAAAAGGGTTCACCTTTACAAGGAGCCGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF647 Mito_carr

Protein Analysis

360

Amino Acids

39.54

Weight (kDa)

8.87

Isoelectric Point (pI)

30.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mito_carr PF00153 23 - 114 5.9e-21 Mitochondrial carrier protein
Mito_carr PF00153 122 - 228 9.9e-18 Mitochondrial carrier protein
Mito_carr PF00153 246 - 331 1.8e-23 Mitochondrial carrier protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010862)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G74240 AT1G74240
fragaria_vesca FvH4_5g13870 FvH4_5g13870 FvH4_5g13870
malus_domestica MD06G1202800.v1.1 MD14G1211900.v1.1
prunus_persica Prupe.5G204700_v2.0.a1
pyrus_communis pycom14g17550
rosa_chinensis RchiOBHm_Chr7g0183061
rosa_laevigata RLG00000005082
rosa_multiflora Rmu_sc0000014.1_g000003
rosa_roxburghii Rroxscaffold_3G00270800
rosa_rugosa Rorug06G0452200 Rorug06G0452300
rosa_samantha Rh7AG055700 Rh7BG055000 Rh7CG056400 Rh7DG055200
rosa_wichuraiana Rw7G004530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 442
AccB7I CCANNNNNTGG 1 cut(s) 330
AccBSI CCGCTC 1 cut(s) 218
AccI GTMKAC 2 cut(s) 135, 320
AciI CCGC 2 cut(s) 218, 1074
AclWI GGATC 3 cut(s) 707, 720, 841
AcoI YGGCCR 1 cut(s) 966
AcsI RAATTY 3 cut(s) 19, 718, 974
AcuI CTGAAG 2 cut(s) 188, 933
AfaI GTAC 4 cut(s) 213, 753, 828, 848
AfiI CCNNNNNNNGG 3 cut(s) 330, 641, 1067
AgsI TTSAA 3 cut(s) 338, 458, 673
AjiI CACGTC 1 cut(s) 631
AjnI CCWGG 3 cut(s) 162, 514, 923
AluBI AGCT 5 cut(s) 15, 230, 263, 389, 947
AluI AGCT 5 cut(s) 15, 230, 263, 389, 947
Alw21I GWGCWC 1 cut(s) 17
AlwI GGATC 3 cut(s) 707, 720, 841
Ama87I CYCGRG 1 cut(s) 1020
AoxI GGCC 3 cut(s) 468, 885, 966
ApeKI GCWGC 1 cut(s) 561
ApoI RAATTY 3 cut(s) 19, 718, 974
ArsI GACNNNNNNTTYG 2 cut(s) 620, 652
AspS9I GGNCC 2 cut(s) 612, 885
AsuC2I CCSGG 1 cut(s) 267
AsuHPI GGTGA 1 cut(s) 1050
AsuNHI GCTAGC 1 cut(s) 1031
AvaI CYCGRG 1 cut(s) 1020
AvaII GGWCC 1 cut(s) 612
BaeGI GKGCMC 1 cut(s) 373
BamHI GGATCC 1 cut(s) 712
BanII GRGCYC 1 cut(s) 17
BarI GAAGNNNNNNTAC 2 cut(s) 686, 718
BbsI GAAGAC 1 cut(s) 200
Bbv12I GWGCWC 1 cut(s) 17
BbvI GCAGC 1 cut(s) 548
BccI CCATC 3 cut(s) 202, 227, 460
BceAI ACGGC 2 cut(s) 47, 953
BcgI CGANNNNNNTGC 2 cut(s) 17, 51
BciT130I CCWGG 3 cut(s) 164, 516, 925
BciVI GTATCC 2 cut(s) 40, 599
BclI TGATCA 1 cut(s) 807
BcnI CCSGG 1 cut(s) 267
BfaI CTAG 2 cut(s) 755, 1032
BfuAI ACCTGC 1 cut(s) 442
BfuI GTATCC 2 cut(s) 40, 599
BisI GCNGC 2 cut(s) 562, 1074
BlsI GCNGC 2 cut(s) 563, 1075
Bme1390I CCNGG 4 cut(s) 164, 267, 516, 925
Bme18I GGWCC 1 cut(s) 612
BmeT110I CYCGRG 1 cut(s) 1020
BmgBI CACGTC 1 cut(s) 631
BmgT120I GGNCC 2 cut(s) 612, 885
BmiI GGNNCC 2 cut(s) 714, 1072
BmrFI CCNGG 4 cut(s) 164, 267, 516, 925
BmrI ACTGGG 1 cut(s) 552
BmsI GCATC 3 cut(s) 114, 136, 853
BmtI GCTAGC 1 cut(s) 1035
BmuI ACTGGG 1 cut(s) 552
BpiI GAAGAC 1 cut(s) 200
BpmI CTGGAG 3 cut(s) 123, 312, 405
BpuEI CTTGAG 2 cut(s) 377, 862
BpuMI CCSGG 1 cut(s) 267
BsaBI GATNNNNATC 1 cut(s) 51
BsaJI CCNNGG 3 cut(s) 798, 923, 969
BsaWI WCCGGW 1 cut(s) 130
BsaXI ACNNNNNCTCC 2 cut(s) 71, 101
Bsc4I CCNNNNNNNGG 3 cut(s) 330, 641, 1067
Bse1I ACTGG 5 cut(s) 280, 295, 547, 614, 624
Bse3DI GCAATG 1 cut(s) 378
Bse8I GATNNNNATC 1 cut(s) 51
BseBI CCWGG 3 cut(s) 164, 516, 925
BseDI CCNNGG 3 cut(s) 798, 923, 969
BseGI GGATG 6 cut(s) 127, 346, 552, 808, 868, 909
BseJI GATNNNNATC 1 cut(s) 51
BseLI CCNNNNNNNGG 3 cut(s) 330, 641, 1067
BseMI GCAATG 1 cut(s) 378
BseMII CTCAG 1 cut(s) 994
BseNI ACTGG 5 cut(s) 280, 295, 547, 614, 624
BseSI GKGCMC 1 cut(s) 373
BseXI GCAGC 1 cut(s) 548
Bsh1285I CGRYCG 1 cut(s) 135
BshFI GGCC 3 cut(s) 470, 887, 968
BsiEI CGRYCG 1 cut(s) 135
BsiHKAI GWGCWC 1 cut(s) 17
BsiHKCI CYCGRG 1 cut(s) 1020
BsiSI CCGG 2 cut(s) 131, 266
BslFI GGGAC 3 cut(s) 375, 641, 905
BslI CCNNNNNNNGG 3 cut(s) 330, 641, 1067
BsmFI GGGAC 3 cut(s) 375, 641, 905
BsmI GAATGC 1 cut(s) 153
BsnI GGCC 3 cut(s) 470, 887, 968
BsoBI CYCGRG 1 cut(s) 1020
Bsp1286I GDGCHC 2 cut(s) 17, 373
Bsp143I GATC 3 cut(s) 712, 807, 833
BspACI CCGC 2 cut(s) 218, 1074
BspANI GGCC 3 cut(s) 470, 887, 968
BspCNI CTCAG 1 cut(s) 993
BspHI TCATGA 1 cut(s) 489
BspLI GGNNCC 2 cut(s) 714, 1072
BspMI ACCTGC 1 cut(s) 442
BspOI GCTAGC 1 cut(s) 1035
BspPI GGATC 3 cut(s) 707, 720, 841
BsrBI CCGCTC 1 cut(s) 218
BsrDI GCAATG 1 cut(s) 378
BsrI ACTGG 5 cut(s) 280, 295, 547, 614, 624
BssECI CCNNGG 3 cut(s) 798, 923, 969
BssMI GATC 3 cut(s) 712, 807, 833
BssT1I CCWWGG 1 cut(s) 798
Bst2UI CCWGG 3 cut(s) 164, 516, 925
Bst4CI ACNGT 1 cut(s) 142
BstAPI GCANNNNNTGC 1 cut(s) 448
BstC8I GCNNGC 4 cut(s) 36, 449, 559, 1033
BstDEI CTNAG 2 cut(s) 587, 980
BstDSI CCRYGG 1 cut(s) 969
BstENI CCTNNNNNAGG 1 cut(s) 1065
BstF5I GGATG 6 cut(s) 127, 346, 552, 808, 868, 909
BstKTI GATC 3 cut(s) 715, 810, 836
BstMBI GATC 3 cut(s) 712, 807, 833
BstMCI CGRYCG 1 cut(s) 135
BstMWI GCNNNNNNNGC 5 cut(s) 159, 293, 448, 694, 893
BstNI CCWGG 3 cut(s) 164, 516, 925
BstNSI RCATGY 2 cut(s) 38, 451
BstSCI CCNGG 4 cut(s) 162, 265, 514, 923
BstSLI GKGCMC 1 cut(s) 373
BstV1I GCAGC 1 cut(s) 548
BstV2I GAAGAC 1 cut(s) 200
BstX2I RGATCY 1 cut(s) 712
BstXI CCANNNNNNTGG 2 cut(s) 530, 799
BstYI RGATCY 1 cut(s) 712
BsuI GTATCC 2 cut(s) 40, 599
BsuRI GGCC 3 cut(s) 470, 887, 968
BtgI CCRYGG 1 cut(s) 969
BtrI CACGTC 1 cut(s) 631
BtsCI GGATG 6 cut(s) 127, 346, 552, 808, 868, 909
BtsIMutI CAGTG 3 cut(s) 273, 617, 1047
BveI ACCTGC 1 cut(s) 442
Cac8I GCNNGC 4 cut(s) 36, 449, 559, 1033
CciI TCATGA 1 cut(s) 489
Cfr13I GGNCC 2 cut(s) 612, 885
CseI GACGC 1 cut(s) 907
Csp6I GTAC 4 cut(s) 212, 752, 827, 847
CviAII CATG 7 cut(s) 35, 426, 448, 490, 554, 689, 933
CviQI GTAC 4 cut(s) 212, 752, 827, 847
DdeI CTNAG 2 cut(s) 587, 980
DpnI GATC 3 cut(s) 714, 809, 835
DpnII GATC 3 cut(s) 712, 807, 833
EaeI YGGCCR 1 cut(s) 966
Ecl136II GAGCTC 1 cut(s) 15
Eco130I CCWWGG 1 cut(s) 798
Eco24I GRGCYC 1 cut(s) 17
Eco47I GGWCC 1 cut(s) 612
Eco53kI GAGCTC 1 cut(s) 15
Eco57I CTGAAG 2 cut(s) 188, 933
Eco88I CYCGRG 1 cut(s) 1020
EcoICRI GAGCTC 1 cut(s) 15
EcoNI CCTNNNNNAGG 1 cut(s) 1065
EcoRI GAATTC 1 cut(s) 19
EcoRII CCWGG 3 cut(s) 162, 514, 923
EcoT14I CCWWGG 1 cut(s) 798
EcoT22I ATGCAT 1 cut(s) 129
EcoT38I GRGCYC 1 cut(s) 17
ErhI CCWWGG 1 cut(s) 798
FaeI CATG 7 cut(s) 38, 429, 451, 493, 557, 692, 936
FaqI GGGAC 3 cut(s) 375, 641, 905
FatI CATG 7 cut(s) 34, 425, 447, 489, 553, 688, 932
FbaI TGATCA 1 cut(s) 807
FblI GTMKAC 2 cut(s) 135, 320
Fnu4HI GCNGC 2 cut(s) 562, 1074
FokI GGATG 6 cut(s) 114, 333, 559, 815, 875, 916
FriOI GRGCYC 1 cut(s) 17
Fsp4HI GCNGC 2 cut(s) 562, 1074
FspBI CTAG 2 cut(s) 755, 1032
GluI GCNGC 2 cut(s) 562, 1074
GsuI CTGGAG 3 cut(s) 123, 312, 405
HaeIII GGCC 3 cut(s) 470, 887, 968
HapII CCGG 2 cut(s) 131, 266
HgaI GACGC 1 cut(s) 907
Hin1II CATG 7 cut(s) 38, 429, 451, 493, 557, 692, 936
HincII GTYRAC 2 cut(s) 136, 321
HindII GTYRAC 2 cut(s) 136, 321
HinfI GANTC 3 cut(s) 52, 317, 341
HpaII CCGG 2 cut(s) 131, 266
HphI GGTGA 1 cut(s) 1050
Hpy166II GTNNAC 4 cut(s) 136, 321, 615, 1058
Hpy188I TCNGA 6 cut(s) 46, 159, 186, 207, 911, 983
Hpy188III TCNNGA 3 cut(s) 356, 490, 1022
Hpy8I GTNNAC 4 cut(s) 136, 321, 615, 1058
Hpy99I CGWCG 1 cut(s) 998
HpyAV CCTTC 5 cut(s) 110, 568, 659, 884, 992
HpyCH4III ACNGT 1 cut(s) 142
HpyCH4IV ACGT 3 cut(s) 630, 876, 959
HpyCH4V TGCA 8 cut(s) 127, 179, 287, 451, 602, 823, 866, 896
HpyF10VI GCNNNNNNNGC 5 cut(s) 159, 293, 448, 694, 893
HpyF3I CTNAG 2 cut(s) 587, 980
HpySE526I ACGT 3 cut(s) 630, 876, 959
Hsp92II CATG 7 cut(s) 38, 429, 451, 493, 557, 692, 936
Ksp22I TGATCA 1 cut(s) 807
Kzo9I GATC 3 cut(s) 712, 807, 833
LmnI GCTCC 7 cut(s) 92, 104, 268, 293, 386, 577, 1070
Lsp1109I GCAGC 1 cut(s) 548
LweI GCATC 3 cut(s) 114, 136, 853
MaeI CTAG 2 cut(s) 755, 1032
MaeII ACGT 3 cut(s) 630, 876, 959
MaeIII GTNAC 2 cut(s) 271, 533
MalI GATC 3 cut(s) 714, 809, 835
MbiI CCGCTC 1 cut(s) 218
MboI GATC 3 cut(s) 712, 807, 833
MboII GAAGA 3 cut(s) 58, 205, 350
MflI RGATCY 1 cut(s) 712
MhlI GDGCHC 2 cut(s) 17, 373
MluCI AATT 5 cut(s) 19, 168, 718, 867, 974
MlyI GAGTC 1 cut(s) 326
MmeI TCCRAC 2 cut(s) 373, 840
Mph1103I ATGCAT 1 cut(s) 129
MseI TTAA 3 cut(s) 239, 647, 729
MspI CCGG 2 cut(s) 131, 266
MspR9I CCNGG 4 cut(s) 164, 267, 516, 925
Mva1269I GAATGC 1 cut(s) 153
MvaI CCWGG 3 cut(s) 164, 516, 925
MwoI GCNNNNNNNGC 5 cut(s) 159, 293, 448, 694, 893
NciI CCSGG 1 cut(s) 267
NdeII GATC 3 cut(s) 712, 807, 833
NheI GCTAGC 1 cut(s) 1031
NlaIII CATG 7 cut(s) 38, 429, 451, 493, 557, 692, 936
NlaIV GGNNCC 2 cut(s) 714, 1072
NmuCI GTSAC 1 cut(s) 271
NsiI ATGCAT 1 cut(s) 129
NspI RCATGY 2 cut(s) 38, 451
PaeI GCATGC 2 cut(s) 38, 451
PaeR7I CTCGAG 1 cut(s) 1020
PagI TCATGA 1 cut(s) 489
PctI GAATGC 1 cut(s) 153
PfeI GAWTC 2 cut(s) 52, 341
PflMI CCANNNNNTGG 1 cut(s) 330
PkrI GCNGC 2 cut(s) 563, 1075
PleI GAGTC 1 cut(s) 325
PpsI GAGTC 1 cut(s) 325
Psp124BI GAGCTC 1 cut(s) 17
Psp6I CCWGG 3 cut(s) 162, 514, 923
PspGI CCWGG 3 cut(s) 162, 514, 923
PspN4I GGNNCC 2 cut(s) 714, 1072
PspPI GGNCC 2 cut(s) 612, 885
PsuI RGATCY 1 cut(s) 712
RsaI GTAC 4 cut(s) 213, 753, 828, 848
RsaNI GTAC 4 cut(s) 212, 752, 827, 847
SacI GAGCTC 1 cut(s) 17
SalI GTCGAC 2 cut(s) 134, 319
SaqAI TTAA 3 cut(s) 239, 647, 729
SatI GCNGC 2 cut(s) 562, 1074
Sau3AI GATC 3 cut(s) 712, 807, 833
Sau96I GGNCC 2 cut(s) 612, 885
SchI GAGTC 1 cut(s) 326
ScrFI CCNGG 4 cut(s) 164, 267, 516, 925
SduI GDGCHC 2 cut(s) 17, 373
SfaNI GCATC 3 cut(s) 114, 136, 853
Sfr274I CTCGAG 1 cut(s) 1020
SinI GGWCC 1 cut(s) 612
SlaI CTCGAG 1 cut(s) 1020
SmlI CTYRAG 3 cut(s) 356, 841, 1020
SmoI CTYRAG 3 cut(s) 356, 841, 1020
SphI GCATGC 2 cut(s) 38, 451
Sse9I AATT 5 cut(s) 19, 168, 718, 867, 974
SsiI CCGC 2 cut(s) 218, 1074
SspMI CTAG 2 cut(s) 755, 1032
SstI GAGCTC 1 cut(s) 17
StyD4I CCNGG 4 cut(s) 162, 265, 514, 923
StyI CCWWGG 1 cut(s) 798
TaaI ACNGT 1 cut(s) 142
TaiI ACGT 3 cut(s) 633, 879, 962
TaqI TCGA 7 cut(s) 17, 23, 135, 320, 741, 993, 1021
TasI AATT 5 cut(s) 19, 168, 718, 867, 974
TatI WGTACW 2 cut(s) 751, 826
TauI GCSGC 1 cut(s) 1076
TfiI GAWTC 2 cut(s) 52, 341
Tru1I TTAA 3 cut(s) 239, 647, 729
Tru9I TTAA 3 cut(s) 239, 647, 729
TscAI CASTG 3 cut(s) 280, 624, 1047
TseFI GTSAC 1 cut(s) 271
TseI GCWGC 1 cut(s) 561
Tsp45I GTSAC 1 cut(s) 271
TspDTI ATGAA 4 cut(s) 367, 452, 506, 524
TspRI CASTG 3 cut(s) 280, 624, 1047
Van91I CCANNNNNTGG 1 cut(s) 330
VpaK11BI GGWCC 1 cut(s) 612
XagI CCTNNNNNAGG 1 cut(s) 1065
XapI RAATTY 3 cut(s) 19, 718, 974
XceI RCATGY 2 cut(s) 38, 451
XcmI CCANNNNNNNNNTGG 1 cut(s) 205
XhoI CTCGAG 1 cut(s) 1020
XmiI GTMKAC 2 cut(s) 135, 320
XspI CTAG 2 cut(s) 755, 1032
Zsp2I ATGCAT 1 cut(s) 129
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.