AT1G74640

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0014291
Physical Location & Seq
Forward (+)
28032562 .. 28034863
2302 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G74640.1

Sequence Viewer

Length: 1113 bp
ATGGCTGCTTCAGCTGCATTCTCCATTTCATCACCACCTTTTGTACCCTTTACCACCAAAATTCGCAGACCATTTCTCTTGCTTACTCCAAAATATCCCAAACCTGTTTCGGTGCGAGCTTCTGTGTCCTCGATCTCCATCGATGAAAAATTGCCAGTCCAAACAAAGACAAGCAACTGGCAGTGGAAGTTCAAGGGCAATTCGATTGGTATATACTATGAAGAGCATGAGAGGGAGAAGTGTGAATCGGCTAAGAATATTCTAATGATACCAACAATTTCCGATGTTAGCACCGTTGAGGAGTGGAGATCTGTAGCTAAAGACATTGTGCAGCGCGATGGAGACGTCAACTGGCGAGCAACTATTGTAGATTGGCCCGGTCTTGGTTACTCTGCTAGGCCGAAGATGGATTATGACACAGATGTCATGGAGAAGTTTGTGGTCGACTTCATGAACTCACCTGAAAGCCCAATGAGCCAGTCAGGTAATGATGATTTAGTGATCATTGGAGGAGGGCATGCGGCAACACTAGCACTCCGTGCTACCCAACGTGGGCTACTAAAACCATCAGCTATTGCTGCTGTCGCACCTACATGGGCTGGACCTCTCCCTATTGTATTTGGCCGTGATTCTTCCATGGTGTCGAGGTATGGTATGCTAAGAGGAACTCTAAGAGCACCTGGAGTTGGTTGGATGATGTACAACATGTTGGTGAGCAACGAGAAGTCAATCGAGTCTCAGTACAAATCCCATGTTTATGCAGACCAAACCAACGTGACCGATGCCATAATCCAAAGCAGATACGAGTTAACAAAACAGAAGGGATCACGCTACGTCCCTGCAGCTTTTCTGACTGGTTTACTCGACCCGGTATCATCCCGGGAAGAATTTCTCCAACTGTTTGCTGATTTGGAAGGGAAATTACCGGTTATGGTTATGTCAACAAAAGGAGCTCCAAAAAGATCGAAAGCAGAAATGGAAGCATTAAGAGGAGCGAAAGGAGTAAGCAAATTCGTGGAAGTTGAAGGTGCACTCTTACCTCAAGAAGAGTATCCTTCTCTTGTTGCTCAAGAGCTCTACAACTTCTTGCAAGAGACTTTTGCTAAATGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_6

Protein Analysis

370

Amino Acids

41.06

Weight (kDa)

8.3

Isoelectric Point (pI)

47.72

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014291)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G74640
fragaria_vesca FvH4_5g14981
malus_domestica MD14G1199800.v1.1
prunus_persica Prupe.5G193100_v2.0.a1
pyrus_communis pycom14g16570
rosa_chinensis RchiOBHm_Chr7g0181351
rosa_laevigata RLG00000005196
rosa_multiflora Rmu_co8287351.1_g000001 Rmu_co8345699.1_g000001 Rmu_sc0013944.1_g000001
rosa_roxburghii Rroxscaffold_3G00272110
rosa_rugosa Rorug06G0440400
rosa_samantha Rh7AG042400 Rh7BG041300 Rh7CG043700 Rh7DG042200
rosa_wichuraiana Rw7G003500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 422
AatII GACGTC 1 cut(s) 348
AccI GTMKAC 1 cut(s) 444
AccII CGCG 1 cut(s) 336
AciI CCGC 1 cut(s) 521
AclWI GGATC 1 cut(s) 832
AcoI YGGCCR 1 cut(s) 622
AcsI RAATTY 3 cut(s) 60, 887, 1010
AcyI GRCGYC 1 cut(s) 345
AdeI CACNNNGTG 1 cut(s) 539
AfaI GTAC 3 cut(s) 45, 701, 743
AfiI CCNNNNNNNGG 3 cut(s) 383, 552, 686
AflIII ACRYGT 1 cut(s) 705
AgeI ACCGGT 1 cut(s) 925
AgsI TTSAA 2 cut(s) 193, 1025
AjnI CCWGG 1 cut(s) 679
AluBI AGCT 7 cut(s) 14, 119, 317, 572, 845, 953, 1075
AluI AGCT 7 cut(s) 14, 119, 317, 572, 845, 953, 1075
Alw21I GWGCWC 4 cut(s) 679, 955, 1033, 1077
Alw26I GTCTC 3 cut(s) 336, 741, 1088
Alw44I GTGCAC 1 cut(s) 1029
AlwI GGATC 1 cut(s) 832
Ama87I CYCGRG 1 cut(s) 879
AoxI GGCC 3 cut(s) 374, 398, 622
ApaLI GTGCAC 1 cut(s) 1029
ApeKI GCWGC 5 cut(s) 5, 14, 331, 578, 842
ApoI RAATTY 3 cut(s) 60, 887, 1010
AsiGI ACCGGT 1 cut(s) 925
Asp700I GAANNNNTTC 1 cut(s) 888
AspLEI GCGC 1 cut(s) 336
AspS9I GGNCC 2 cut(s) 375, 602
AsuC2I CCSGG 4 cut(s) 378, 869, 880, 881
AsuHPI GGTGA 3 cut(s) 24, 450, 724
AvaI CYCGRG 1 cut(s) 879
AvaII GGWCC 1 cut(s) 602
BaeGI GKGCMC 1 cut(s) 1033
BanII GRGCYC 2 cut(s) 955, 1077
BarI GAAGNNNNNNTAC 2 cut(s) 906, 938
Bbv12I GWGCWC 4 cut(s) 679, 955, 1033, 1077
BbvI GCAGC 3 cut(s) 343, 565, 854
BccI CCATC 4 cut(s) 146, 332, 400, 574
BceAI ACGGC 1 cut(s) 609
BciT130I CCWGG 1 cut(s) 681
BciVI GTATCC 1 cut(s) 1062
BclI TGATCA 1 cut(s) 501
BcnI CCSGG 4 cut(s) 378, 869, 880, 881
BcoDI GTCTC 3 cut(s) 336, 741, 1088
BfaI CTAG 2 cut(s) 396, 530
BfmI CTRYAG 2 cut(s) 312, 840
BfuI GTATCC 1 cut(s) 1062
BglII AGATCT 1 cut(s) 308
BisI GCNGC 6 cut(s) 6, 15, 332, 522, 579, 843
BlsI GCNGC 6 cut(s) 7, 16, 333, 523, 580, 844
Bme1390I CCNGG 5 cut(s) 378, 681, 869, 880, 881
Bme18I GGWCC 1 cut(s) 602
BmeT110I CYCGRG 1 cut(s) 879
BmgT120I GGNCC 2 cut(s) 375, 602
BmrFI CCNGG 5 cut(s) 378, 681, 869, 880, 881
BmsI GCATC 1 cut(s) 772
BpmI CTGGAG 1 cut(s) 702
BpuEI CTTGAG 2 cut(s) 1026, 1053
BpuMI CCSGG 4 cut(s) 378, 869, 880, 881
Bsa29I ATCGAT 1 cut(s) 141
BsaBI GATNNNNATC 1 cut(s) 137
BsaHI GRCGYC 1 cut(s) 345
BsaJI CCNNGG 2 cut(s) 636, 879
BsaWI WCCGGW 1 cut(s) 925
BsaXI ACNNNNNCTCC 2 cut(s) 519, 549
Bsc4I CCNNNNNNNGG 3 cut(s) 383, 552, 686
Bse118I RCCGGY 1 cut(s) 925
Bse1I ACTGG 5 cut(s) 155, 182, 356, 478, 859
Bse8I GATNNNNATC 1 cut(s) 137
BseBI CCWGG 1 cut(s) 681
BseCI ATCGAT 1 cut(s) 141
BseDI CCNNGG 2 cut(s) 636, 879
BseGI GGATG 2 cut(s) 699, 875
BseJI GATNNNNATC 1 cut(s) 137
BseLI CCNNNNNNNGG 3 cut(s) 383, 552, 686
BseMII CTCAG 1 cut(s) 752
BseNI ACTGG 5 cut(s) 155, 182, 356, 478, 859
BseRI GAGGAG 3 cut(s) 314, 525, 1005
BseSI GKGCMC 1 cut(s) 1033
BseXI GCAGC 3 cut(s) 343, 565, 854
BsgI GTGCAG 1 cut(s) 350
Bsh1236I CGCG 1 cut(s) 336
BshFI GGCC 3 cut(s) 376, 400, 624
BshTI ACCGGT 1 cut(s) 925
BshVI ATCGAT 1 cut(s) 141
BsiHKAI GWGCWC 4 cut(s) 679, 955, 1033, 1077
BsiHKCI CYCGRG 1 cut(s) 879
BsiSI CCGG 4 cut(s) 378, 869, 880, 926
BslFI GGGAC 1 cut(s) 821
BslI CCNNNNNNNGG 3 cut(s) 383, 552, 686
BsmAI GTCTC 3 cut(s) 336, 741, 1088
BsmBI CGTCTC 1 cut(s) 336
BsmFI GGGAC 1 cut(s) 821
BsmI GAATGC 1 cut(s) 17
BsnI GGCC 3 cut(s) 376, 400, 624
BsoBI CYCGRG 1 cut(s) 879
Bsp1286I GDGCHC 4 cut(s) 679, 955, 1033, 1077
Bsp1407I TGTACA 1 cut(s) 699
Bsp143I GATC 5 cut(s) 132, 308, 501, 824, 962
Bsp19I CCATGG 1 cut(s) 636
BspACI CCGC 1 cut(s) 521
BspANI GGCC 3 cut(s) 376, 400, 624
BspCNI CTCAG 1 cut(s) 751
BspDI ATCGAT 1 cut(s) 141
BspFNI CGCG 1 cut(s) 336
BspHI TCATGA 1 cut(s) 450
BspMAI CTGCAG 1 cut(s) 844
BspPI GGATC 1 cut(s) 832
BspQI GCTCTTC 1 cut(s) 216
BsrFI RCCGGY 1 cut(s) 925
BsrGI TGTACA 1 cut(s) 699
BsrI ACTGG 5 cut(s) 155, 182, 356, 478, 859
BssAI RCCGGY 1 cut(s) 925
BssECI CCNNGG 2 cut(s) 636, 879
BssMI GATC 5 cut(s) 132, 308, 501, 824, 962
BssNI GRCGYC 1 cut(s) 345
BssT1I CCWWGG 1 cut(s) 636
Bst2UI CCWGG 1 cut(s) 681
Bst4CI ACNGT 2 cut(s) 295, 900
Bst6I CTCTTC 2 cut(s) 216, 1041
BstACI GRCGYC 1 cut(s) 345
BstAPI GCANNNNNTGC 1 cut(s) 539
BstAUI TGTACA 1 cut(s) 699
BstC8I GCNNGC 3 cut(s) 117, 357, 519
BstDEI CTNAG 4 cut(s) 252, 659, 671, 738
BstDSI CCRYGG 1 cut(s) 636
BstF5I GGATG 2 cut(s) 699, 875
BstFNI CGCG 1 cut(s) 336
BstHHI GCGC 1 cut(s) 336
BstKTI GATC 5 cut(s) 135, 311, 504, 827, 965
BstMAI GTCTC 3 cut(s) 336, 741, 1088
BstMBI GATC 5 cut(s) 132, 308, 501, 824, 962
BstMWI GCNNNNNNNGC 7 cut(s) 11, 14, 474, 530, 539, 578, 584
BstNI CCWGG 1 cut(s) 681
BstNSI RCATGY 2 cut(s) 521, 709
BstSCI CCNGG 5 cut(s) 376, 679, 867, 878, 879
BstSFI CTRYAG 2 cut(s) 312, 840
BstSLI GKGCMC 1 cut(s) 1033
BstUI CGCG 1 cut(s) 336
BstV1I GCAGC 3 cut(s) 343, 565, 854
BstX2I RGATCY 1 cut(s) 308
BstYI RGATCY 1 cut(s) 308
Bsu15I ATCGAT 1 cut(s) 141
BsuI GTATCC 1 cut(s) 1062
BsuRI GGCC 3 cut(s) 376, 400, 624
BsuTUI ATCGAT 1 cut(s) 141
BtgI CCRYGG 1 cut(s) 636
BtgZI GCGATG 1 cut(s) 351
BtsCI GGATG 2 cut(s) 699, 875
BtsI GCAGTG 1 cut(s) 188
BtsIMutI CAGTG 1 cut(s) 188
Cac8I GCNNGC 3 cut(s) 117, 357, 519
CciI TCATGA 1 cut(s) 450
CfoI GCGC 1 cut(s) 336
Cfr10I RCCGGY 1 cut(s) 925
Cfr13I GGNCC 2 cut(s) 375, 602
Cfr9I CCCGGG 1 cut(s) 879
ClaI ATCGAT 1 cut(s) 141
Csp6I GTAC 3 cut(s) 44, 700, 742
CspAI ACCGGT 1 cut(s) 925
CviAII CATG 8 cut(s) 227, 427, 451, 518, 594, 637, 706, 752
CviQI GTAC 3 cut(s) 44, 700, 742
DdeI CTNAG 4 cut(s) 252, 659, 671, 738
DpnI GATC 5 cut(s) 134, 310, 503, 826, 964
DpnII GATC 5 cut(s) 132, 308, 501, 824, 962
DraIII CACNNNGTG 1 cut(s) 539
DrdI GACNNNNNNGTC 1 cut(s) 422
DseDI GACNNNNNNGTC 1 cut(s) 422
EaeI YGGCCR 1 cut(s) 622
Eam1104I CTCTTC 2 cut(s) 216, 1041
EarI CTCTTC 2 cut(s) 216, 1041
Ecl136II GAGCTC 2 cut(s) 953, 1075
Eco130I CCWWGG 1 cut(s) 636
Eco24I GRGCYC 2 cut(s) 955, 1077
Eco47I GGWCC 1 cut(s) 602
Eco53kI GAGCTC 2 cut(s) 953, 1075
Eco88I CYCGRG 1 cut(s) 879
EcoICRI GAGCTC 2 cut(s) 953, 1075
EcoRII CCWGG 1 cut(s) 679
EcoT14I CCWWGG 1 cut(s) 636
EcoT38I GRGCYC 2 cut(s) 955, 1077
ErhI CCWWGG 1 cut(s) 636
Esp3I CGTCTC 1 cut(s) 336
FaeI CATG 8 cut(s) 230, 430, 454, 521, 597, 640, 709, 755
FaqI GGGAC 1 cut(s) 821
FatI CATG 8 cut(s) 226, 426, 450, 517, 593, 636, 705, 751
FbaI TGATCA 1 cut(s) 501
FblI GTMKAC 1 cut(s) 444
Fnu4HI GCNGC 6 cut(s) 6, 15, 332, 522, 579, 843
FokI GGATG 2 cut(s) 706, 862
FriOI GRGCYC 2 cut(s) 955, 1077
Fsp4HI GCNGC 6 cut(s) 6, 15, 332, 522, 579, 843
FspBI CTAG 2 cut(s) 396, 530
GlaI GCGC 1 cut(s) 335
GluI GCNGC 6 cut(s) 6, 15, 332, 522, 579, 843
GsuI CTGGAG 1 cut(s) 702
HaeIII GGCC 3 cut(s) 376, 400, 624
HapII CCGG 4 cut(s) 378, 869, 880, 926
HhaI GCGC 1 cut(s) 336
Hin1I GRCGYC 1 cut(s) 345
Hin1II CATG 8 cut(s) 230, 430, 454, 521, 597, 640, 709, 755
Hin6I GCGC 1 cut(s) 334
HinP1I GCGC 1 cut(s) 334
HincII GTYRAC 4 cut(s) 349, 445, 810, 942
HindII GTYRAC 4 cut(s) 349, 445, 810, 942
HinfI GANTC 3 cut(s) 245, 629, 734
HpaI GTTAAC 1 cut(s) 810
HpaII CCGG 4 cut(s) 378, 869, 880, 926
HphI GGTGA 3 cut(s) 24, 450, 724
Hpy166II GTNNAC 6 cut(s) 349, 445, 810, 860, 942, 1031
Hpy188I TCNGA 2 cut(s) 283, 852
Hpy188III TCNNGA 3 cut(s) 451, 1043, 1070
Hpy8I GTNNAC 6 cut(s) 349, 445, 810, 860, 942, 1031
HpyAV CCTTC 4 cut(s) 814, 908, 1019, 1065
HpyCH4III ACNGT 2 cut(s) 295, 900
HpyCH4IV ACGT 4 cut(s) 345, 550, 774, 834
HpyCH4V TGCA 6 cut(s) 17, 331, 761, 842, 1031, 1090
HpyF10VI GCNNNNNNNGC 7 cut(s) 11, 14, 474, 530, 539, 578, 584
HpyF3I CTNAG 4 cut(s) 252, 659, 671, 738
HpySE526I ACGT 4 cut(s) 345, 550, 774, 834
Hsp92I GRCGYC 1 cut(s) 345
Hsp92II CATG 8 cut(s) 230, 430, 454, 521, 597, 640, 709, 755
HspAI GCGC 1 cut(s) 334
Ksp22I TGATCA 1 cut(s) 501
KspAI GTTAAC 1 cut(s) 810
Kzo9I GATC 5 cut(s) 132, 308, 501, 824, 962
LguI GCTCTTC 1 cut(s) 216
LmnI GCTCC 3 cut(s) 950, 958, 992
Lsp1109I GCAGC 3 cut(s) 343, 565, 854
LweI GCATC 1 cut(s) 772
MaeI CTAG 2 cut(s) 396, 530
MaeII ACGT 4 cut(s) 345, 550, 774, 834
MaeIII GTNAC 2 cut(s) 386, 775
MalI GATC 5 cut(s) 134, 310, 503, 826, 964
MboI GATC 5 cut(s) 132, 308, 501, 824, 962
MboII GAAGA 5 cut(s) 233, 415, 624, 896, 1058
MflI RGATCY 1 cut(s) 308
MhlI GDGCHC 4 cut(s) 679, 955, 1033, 1077
MluCI AATT 7 cut(s) 60, 149, 199, 276, 887, 920, 1010
MlyI GAGTC 1 cut(s) 743
MmeI TCCRAC 2 cut(s) 671, 919
MroXI GAANNNNTTC 1 cut(s) 888
MseI TTAA 2 cut(s) 809, 986
MslI CAYNNNNRTG 3 cut(s) 592, 710, 756
MspA1I CMGCKG 1 cut(s) 14
MspI CCGG 4 cut(s) 378, 869, 880, 926
MspR9I CCNGG 5 cut(s) 378, 681, 869, 880, 881
Mva1269I GAATGC 1 cut(s) 17
MvaI CCWGG 1 cut(s) 681
MvnI CGCG 1 cut(s) 336
MwoI GCNNNNNNNGC 7 cut(s) 11, 14, 474, 530, 539, 578, 584
NciI CCSGG 4 cut(s) 378, 869, 880, 881
NcoI CCATGG 1 cut(s) 636
NdeII GATC 5 cut(s) 132, 308, 501, 824, 962
NlaIII CATG 8 cut(s) 230, 430, 454, 521, 597, 640, 709, 755
NmuCI GTSAC 1 cut(s) 775
NspI RCATGY 2 cut(s) 521, 709
PaeI GCATGC 1 cut(s) 521
PagI TCATGA 1 cut(s) 450
PciI ACATGT 1 cut(s) 705
PciSI GCTCTTC 1 cut(s) 216
PctI GAATGC 1 cut(s) 17
PdmI GAANNNNTTC 1 cut(s) 888
PfeI GAWTC 2 cut(s) 245, 629
PinAI ACCGGT 1 cut(s) 925
PkrI GCNGC 6 cut(s) 7, 16, 333, 523, 580, 844
PleI GAGTC 1 cut(s) 742
PpsI GAGTC 1 cut(s) 742
PscI ACATGT 1 cut(s) 705
Psp124BI GAGCTC 2 cut(s) 955, 1077
Psp6I CCWGG 1 cut(s) 679
PspGI CCWGG 1 cut(s) 679
PspPI GGNCC 2 cut(s) 375, 602
PstI CTGCAG 1 cut(s) 844
PsuI RGATCY 1 cut(s) 308
PvuII CAGCTG 1 cut(s) 14
RsaI GTAC 3 cut(s) 45, 701, 743
RsaNI GTAC 3 cut(s) 44, 700, 742
RseI CAYNNNNRTG 3 cut(s) 592, 710, 756
SacI GAGCTC 2 cut(s) 955, 1077
SalI GTCGAC 1 cut(s) 443
SapI GCTCTTC 1 cut(s) 216
SaqAI TTAA 2 cut(s) 809, 986
SatI GCNGC 6 cut(s) 6, 15, 332, 522, 579, 843
Sau3AI GATC 5 cut(s) 132, 308, 501, 824, 962
Sau96I GGNCC 2 cut(s) 375, 602
SchI GAGTC 1 cut(s) 743
ScrFI CCNGG 5 cut(s) 378, 681, 869, 880, 881
SduI GDGCHC 4 cut(s) 679, 955, 1033, 1077
SfaNI GCATC 1 cut(s) 772
SfcI CTRYAG 2 cut(s) 312, 840
SinI GGWCC 1 cut(s) 602
SmaI CCCGGG 1 cut(s) 881
SmiMI CAYNNNNRTG 3 cut(s) 592, 710, 756
SmlI CTYRAG 2 cut(s) 1041, 1068
SmoI CTYRAG 2 cut(s) 1041, 1068
SphI GCATGC 1 cut(s) 521
Sse9I AATT 7 cut(s) 60, 149, 199, 276, 887, 920, 1010
SsiI CCGC 1 cut(s) 521
SspI AATATT 1 cut(s) 259
SspMI CTAG 2 cut(s) 396, 530
SstI GAGCTC 2 cut(s) 955, 1077
StyD4I CCNGG 5 cut(s) 376, 679, 867, 878, 879
StyI CCWWGG 1 cut(s) 636
TaaI ACNGT 2 cut(s) 295, 900
TaiI ACGT 4 cut(s) 348, 553, 777, 837
TaqI TCGA 8 cut(s) 131, 141, 203, 444, 644, 732, 864, 965
TaqII GACCGA 1 cut(s) 794
TasI AATT 7 cut(s) 60, 149, 199, 276, 887, 920, 1010
TatI WGTACW 2 cut(s) 699, 741
TauI GCSGC 1 cut(s) 524
TfiI GAWTC 2 cut(s) 245, 629
Tru1I TTAA 2 cut(s) 809, 986
Tru9I TTAA 2 cut(s) 809, 986
TscAI CASTG 1 cut(s) 188
TseFI GTSAC 1 cut(s) 775
TseI GCWGC 5 cut(s) 5, 14, 331, 578, 842
Tsp45I GTSAC 1 cut(s) 775
TspDTI ATGAA 5 cut(s) 18, 159, 234, 439, 467
TspGWI ACGGA 1 cut(s) 527
TspMI CCCGGG 1 cut(s) 879
TspRI CASTG 1 cut(s) 188
VneI GTGCAC 1 cut(s) 1029
VpaK11BI GGWCC 1 cut(s) 602
XapI RAATTY 3 cut(s) 60, 887, 1010
XceI RCATGY 2 cut(s) 521, 709
XmaI CCCGGG 1 cut(s) 879
XmiI GTMKAC 1 cut(s) 444
XmnI GAANNNNTTC 1 cut(s) 888
XspI CTAG 2 cut(s) 396, 530
ZraI GACGTC 1 cut(s) 346
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.