MD14G1199800.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0014291
Physical Location & Seq
Forward (+)
28908903 .. 28911438
2536 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1199800.v1.1.491

Sequence Viewer

Length: 1131 bp
ATGGCGCTCTCTGCAGCATTCTACGCACCTTCACAGGCGTCCCGTGCTCTTTTGTTCCGAACCCGAAAACCGAATTTCTCACAGACTGCTTGCTTCCAGAAAACCCTAACAGTTAGGGCTTCAACCACCCTGGAAAACTACTCCAAGCTCCCTGTGGGTGAGAAATCTATAGCCCCATCAAAGAGCAATAGTTGGCAATGGAAATTCAAGGAAAATTCTATCAACATCTACTATGAGGAGCATGAAAAGGAAAGGGAGGGTACTTCTAAAAACATTCTTTTGATACCAACCATTTCCGATGTAAGCACTGTTGAAGAATGGAGATCCGTAGCAAGTAATCTTGTTCAGCAAGACGGTAATGTTAATTGGCGAGCAACCATTGTTGATTGGCCTGGTCTGGGGTACTCTGATAGACCAAAGATGGACTACACCACTGATGTCATGGAGAAATTTCTGGTTGACTTCATCAATGCACCTGATGGTCCGATAAGTGGCTCAGAAAATGACTTTGTGGTATTTGGAGGAGGGCATGCAGCTACTTTAACTATTCGTGCTGCAAAACGTGGTGCTGTGAAACCTAAAGCCATTGCCGCCATTGCACCCACGTGGGCTGGTCCCCTTCCTATTGTGTTTGGTAGAGATTCTAGCATGGAAACTAGGTATGGACTGCTTCGAGGCACCTTACGAGCCCCTGCTGTGGGCTGGATGATGTATAATATGCTTGTCAGCAATGAGAAGTCAATTGAATCTCAGTACCGATCACATGTCTATTCAAATCCTGACAATGTGACTCCTGGAATTGTCGAAAGTAGATATGCGCTGACAAAGCGGAAGGGAGCTCGCTACGCGCCCGCTGCTTTCTTGACTGGCCTCCTTGATCCTGTGCAAACCCGAGAGGAATTTCTTGAACTCTTTGCAGATATGGGCAGGAGGAAGATACCAGTTATTGTCCTCTCAACCGAAGGATCTCCAAAGAGGTCAAAAGCAGAAATGGAAGCTCTCCGGGAAGCTGAGGGAGTAAGTAAGTTTGTCGAGGTGCCAGGTGCTCTTCTTCCGCAAGAGGAGTATCCTGCTATGGTTGCAGAGGAGCTTTACCAATTCCTGCAAGAAAGTTTTGAAGTCAACAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_6

Protein Analysis

377

Amino Acids

41.92

Weight (kDa)

6.88

Isoelectric Point (pI)

43.46

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014291)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G74640
fragaria_vesca FvH4_5g14981
malus_domestica MD14G1199800.v1.1
prunus_persica Prupe.5G193100_v2.0.a1
pyrus_communis pycom14g16570
rosa_chinensis RchiOBHm_Chr7g0181351
rosa_laevigata RLG00000005196
rosa_multiflora Rmu_co8287351.1_g000001 Rmu_co8345699.1_g000001 Rmu_sc0013944.1_g000001
rosa_roxburghii Rroxscaffold_3G00272110
rosa_rugosa Rorug06G0440400
rosa_samantha Rh7AG042400 Rh7BG041300 Rh7CG043700 Rh7DG042200
rosa_wichuraiana Rw7G003500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 677, 1036
AccII CGCG 1 cut(s) 848
AciI CCGC 4 cut(s) 591, 829, 852, 1055
AclWI GGATC 3 cut(s) 318, 872, 973
AcsI RAATTY 5 cut(s) 73, 203, 214, 449, 899
AcvI CACGTG 1 cut(s) 606
AcyI GRCGYC 1 cut(s) 38
AfaI GTAC 3 cut(s) 262, 404, 755
AfiI CCNNNNNNNGG 4 cut(s) 398, 491, 697, 698
AflIII ACRYGT 1 cut(s) 763
AgsI TTSAA 7 cut(s) 123, 208, 314, 746, 774, 908, 1118
AjnI CCWGG 4 cut(s) 129, 391, 793, 1039
AleI CACNNNNGTG 1 cut(s) 604
AluBI AGCT 6 cut(s) 148, 536, 839, 998, 1010, 1090
AluI AGCT 6 cut(s) 148, 536, 839, 998, 1010, 1090
Alw21I GWGCWC 3 cut(s) 49, 841, 1048
AlwI GGATC 3 cut(s) 318, 872, 973
Ama87I CYCGRG 1 cut(s) 891
AoxI GGCC 2 cut(s) 389, 868
ApeKI GCWGC 4 cut(s) 14, 533, 554, 854
ApoI RAATTY 5 cut(s) 73, 203, 214, 449, 899
AspLEI GCGC 3 cut(s) 7, 820, 850
AspS9I GGNCC 2 cut(s) 482, 614
AsuC2I CCSGG 1 cut(s) 1004
AsuHPI GGTGA 1 cut(s) 170
AvaI CYCGRG 1 cut(s) 891
AvaII GGWCC 2 cut(s) 482, 614
BanI GGYRCC 2 cut(s) 677, 1036
BanII GRGCYC 2 cut(s) 691, 841
BbrPI CACGTG 1 cut(s) 606
Bbv12I GWGCWC 3 cut(s) 49, 841, 1048
BbvCI CCTCAGC 1 cut(s) 1011
BbvI GCAGC 4 cut(s) 26, 541, 545, 841
BccI CCATC 3 cut(s) 184, 415, 473
BciT130I CCWGG 4 cut(s) 131, 393, 795, 1041
BciVI GTATCC 1 cut(s) 1077
BcnI CCSGG 1 cut(s) 1004
BfaI CTAG 2 cut(s) 645, 657
BfmI CTRYAG 2 cut(s) 12, 168
BfoI RGCGCY 1 cut(s) 8
BfuI GTATCC 1 cut(s) 1077
BisI GCNGC 5 cut(s) 15, 534, 555, 591, 855
BlsI GCNGC 5 cut(s) 16, 535, 556, 592, 856
Bme1390I CCNGG 5 cut(s) 131, 393, 795, 1004, 1041
Bme18I GGWCC 2 cut(s) 482, 614
BmeT110I CYCGRG 1 cut(s) 891
BmgT120I GGNCC 2 cut(s) 482, 614
BmiI GGNNCC 3 cut(s) 616, 679, 1038
BmrFI CCNGG 5 cut(s) 131, 393, 795, 1004, 1041
Bpu10I CCTNAGC 1 cut(s) 1011
BpuMI CCSGG 1 cut(s) 1004
BsaAI YACGTR 1 cut(s) 606
BsaHI GRCGYC 1 cut(s) 38
BsaJI CCNNGG 1 cut(s) 129
Bsc4I CCNNNNNNNGG 4 cut(s) 398, 491, 697, 698
Bse1I ACTGG 2 cut(s) 871, 941
Bse3DI GCAATG 4 cut(s) 203, 585, 594, 736
BseBI CCWGG 4 cut(s) 131, 393, 795, 1041
BseDI CCNNGG 1 cut(s) 129
BseGI GGATG 1 cut(s) 711
BseLI CCNNNNNNNGG 4 cut(s) 398, 491, 697, 698
BseMI GCAATG 4 cut(s) 203, 585, 594, 736
BseMII CTCAG 3 cut(s) 510, 764, 1002
BseNI ACTGG 2 cut(s) 871, 941
BseRI GAGGAG 4 cut(s) 251, 537, 1076, 1100
BseXI GCAGC 4 cut(s) 26, 541, 545, 841
Bsh1236I CGCG 1 cut(s) 848
BshFI GGCC 2 cut(s) 391, 870
BshNI GGYRCC 2 cut(s) 677, 1036
BsiHKAI GWGCWC 3 cut(s) 49, 841, 1048
BsiHKCI CYCGRG 1 cut(s) 891
BsiSI CCGG 1 cut(s) 1003
BslFI GGGAC 2 cut(s) 25, 600
BslI CCNNNNNNNGG 4 cut(s) 398, 491, 697, 698
BsmFI GGGAC 2 cut(s) 25, 600
BsmI GAATGC 1 cut(s) 17
BsnI GGCC 2 cut(s) 391, 870
BsoBI CYCGRG 1 cut(s) 891
Bsp1286I GDGCHC 4 cut(s) 49, 691, 841, 1048
Bsp143I GATC 4 cut(s) 323, 758, 877, 965
BspACI CCGC 4 cut(s) 591, 829, 852, 1055
BspANI GGCC 2 cut(s) 391, 870
BspCNI CTCAG 3 cut(s) 509, 763, 1003
BspFNI CGCG 1 cut(s) 848
BspLI GGNNCC 3 cut(s) 616, 679, 1038
BspMAI CTGCAG 1 cut(s) 16
BspPI GGATC 3 cut(s) 318, 872, 973
BspQI GCTCTTC 1 cut(s) 1053
BspT107I GGYRCC 2 cut(s) 677, 1036
BsrDI GCAATG 4 cut(s) 203, 585, 594, 736
BsrI ACTGG 2 cut(s) 871, 941
BssECI CCNNGG 1 cut(s) 129
BssMI GATC 4 cut(s) 323, 758, 877, 965
BssNI GRCGYC 1 cut(s) 38
Bst2UI CCWGG 4 cut(s) 131, 393, 795, 1041
Bst4CI ACNGT 3 cut(s) 112, 310, 356
Bst6I CTCTTC 1 cut(s) 1053
BstACI GRCGYC 1 cut(s) 38
BstBAI YACGTR 1 cut(s) 606
BstC8I GCNNGC 5 cut(s) 91, 372, 531, 841, 852
BstDEI CTNAG 3 cut(s) 496, 750, 1011
BstF5I GGATG 1 cut(s) 711
BstFNI CGCG 1 cut(s) 848
BstH2I RGCGCY 1 cut(s) 8
BstHHI GCGC 3 cut(s) 7, 820, 850
BstKTI GATC 4 cut(s) 326, 761, 880, 968
BstMBI GATC 4 cut(s) 323, 758, 877, 965
BstMWI GCNNNNNNNGC 9 cut(s) 11, 23, 44, 590, 596, 826, 845, 854, 1079
BstNI CCWGG 4 cut(s) 131, 393, 795, 1041
BstNSI RCATGY 2 cut(s) 533, 767
BstSCI CCNGG 5 cut(s) 129, 391, 793, 1002, 1039
BstSFI CTRYAG 2 cut(s) 12, 168
BstUI CGCG 1 cut(s) 848
BstV1I GCAGC 4 cut(s) 26, 541, 545, 841
BstX2I RGATCY 2 cut(s) 323, 965
BstYI RGATCY 2 cut(s) 323, 965
BsuI GTATCC 1 cut(s) 1077
BsuRI GGCC 2 cut(s) 391, 870
BtsCI GGATG 1 cut(s) 711
BtsIMutI CAGTG 2 cut(s) 306, 432
Cac8I GCNNGC 5 cut(s) 91, 372, 531, 841, 852
CfoI GCGC 3 cut(s) 7, 820, 850
Cfr13I GGNCC 2 cut(s) 482, 614
CseI GACGC 1 cut(s) 27
Csp6I GTAC 3 cut(s) 261, 403, 754
CviAII CATG 5 cut(s) 242, 442, 530, 649, 764
CviQI GTAC 3 cut(s) 261, 403, 754
DdeI CTNAG 3 cut(s) 496, 750, 1011
DpnI GATC 4 cut(s) 325, 760, 879, 967
DpnII GATC 4 cut(s) 323, 758, 877, 965
Eam1104I CTCTTC 1 cut(s) 1053
EarI CTCTTC 1 cut(s) 1053
Ecl136II GAGCTC 1 cut(s) 839
Eco24I GRGCYC 2 cut(s) 691, 841
Eco47I GGWCC 2 cut(s) 482, 614
Eco53kI GAGCTC 1 cut(s) 839
Eco72I CACGTG 1 cut(s) 606
Eco88I CYCGRG 1 cut(s) 891
EcoICRI GAGCTC 1 cut(s) 839
EcoRII CCWGG 4 cut(s) 129, 391, 793, 1039
EcoT38I GRGCYC 2 cut(s) 691, 841
FaeI CATG 5 cut(s) 245, 445, 533, 652, 767
FaqI GGGAC 2 cut(s) 25, 600
FatI CATG 5 cut(s) 241, 441, 529, 648, 763
FauI CCCGC 1 cut(s) 859
Fnu4HI GCNGC 5 cut(s) 15, 534, 555, 591, 855
FokI GGATG 1 cut(s) 718
FriOI GRGCYC 2 cut(s) 691, 841
Fsp4HI GCNGC 5 cut(s) 15, 534, 555, 591, 855
FspBI CTAG 2 cut(s) 645, 657
GlaI GCGC 3 cut(s) 6, 819, 849
GluI GCNGC 5 cut(s) 15, 534, 555, 591, 855
HaeII RGCGCY 1 cut(s) 8
HaeIII GGCC 2 cut(s) 391, 870
HapII CCGG 1 cut(s) 1003
HgaI GACGC 1 cut(s) 27
HhaI GCGC 3 cut(s) 7, 820, 850
Hin1I GRCGYC 1 cut(s) 38
Hin1II CATG 5 cut(s) 245, 445, 533, 652, 767
Hin6I GCGC 3 cut(s) 5, 818, 848
HinP1I GCGC 3 cut(s) 5, 818, 848
HincII GTYRAC 2 cut(s) 460, 1123
HindII GTYRAC 2 cut(s) 460, 1123
HinfI GANTC 3 cut(s) 641, 746, 790
HpaII CCGG 1 cut(s) 1003
HphI GGTGA 1 cut(s) 170
Hpy166II GTNNAC 2 cut(s) 460, 1123
Hpy188I TCNGA 5 cut(s) 59, 298, 409, 486, 499
Hpy188III TCNNGA 4 cut(s) 97, 779, 862, 905
Hpy8I GTNNAC 2 cut(s) 460, 1123
HpyAV CCTTC 4 cut(s) 39, 629, 826, 956
HpyCH4III ACNGT 3 cut(s) 112, 310, 356
HpyCH4IV ACGT 2 cut(s) 562, 605
HpyCH4V TGCA 9 cut(s) 14, 473, 533, 557, 599, 886, 917, 1082, 1105
HpyF10VI GCNNNNNNNGC 9 cut(s) 11, 23, 44, 590, 596, 826, 845, 854, 1079
HpyF3I CTNAG 3 cut(s) 496, 750, 1011
HpySE526I ACGT 2 cut(s) 562, 605
Hsp92I GRCGYC 1 cut(s) 38
Hsp92II CATG 5 cut(s) 245, 445, 533, 652, 767
HspAI GCGC 3 cut(s) 5, 818, 848
Kzo9I GATC 4 cut(s) 323, 758, 877, 965
LguI GCTCTTC 1 cut(s) 1053
LmnI GCTCC 4 cut(s) 153, 238, 836, 1087
Lsp1109I GCAGC 4 cut(s) 26, 541, 545, 841
MaeI CTAG 2 cut(s) 645, 657
MaeII ACGT 2 cut(s) 562, 605
MaeIII GTNAC 1 cut(s) 787
MalI GATC 4 cut(s) 325, 760, 879, 967
MboI GATC 4 cut(s) 323, 758, 877, 965
MboII GAAGA 4 cut(s) 326, 946, 1040, 1043
MfeI CAATTG 1 cut(s) 741
MflI RGATCY 2 cut(s) 323, 965
MhlI GDGCHC 4 cut(s) 49, 691, 841, 1048
MlyI GAGTC 1 cut(s) 784
MseI TTAA 2 cut(s) 363, 542
MslI CAYNNNNRTG 1 cut(s) 604
MspA1I CMGCKG 1 cut(s) 854
MspI CCGG 1 cut(s) 1003
MspR9I CCNGG 5 cut(s) 131, 393, 795, 1004, 1041
MunI CAATTG 1 cut(s) 741
Mva1269I GAATGC 1 cut(s) 17
MvaI CCWGG 4 cut(s) 131, 393, 795, 1041
MvnI CGCG 1 cut(s) 848
MwoI GCNNNNNNNGC 9 cut(s) 11, 23, 44, 590, 596, 826, 845, 854, 1079
NciI CCSGG 1 cut(s) 1004
NdeII GATC 4 cut(s) 323, 758, 877, 965
NlaIII CATG 5 cut(s) 245, 445, 533, 652, 767
NlaIV GGNNCC 3 cut(s) 616, 679, 1038
NmuCI GTSAC 1 cut(s) 787
NspI RCATGY 2 cut(s) 533, 767
OliI CACNNNNGTG 1 cut(s) 604
PaeI GCATGC 1 cut(s) 533
PciI ACATGT 1 cut(s) 763
PciSI GCTCTTC 1 cut(s) 1053
PctI GAATGC 1 cut(s) 17
PfeI GAWTC 2 cut(s) 641, 746
PfoI TCCNGGA 2 cut(s) 793, 1002
PkrI GCNGC 5 cut(s) 16, 535, 556, 592, 856
PleI GAGTC 1 cut(s) 784
PmaCI CACGTG 1 cut(s) 606
PmlI CACGTG 1 cut(s) 606
PpsI GAGTC 1 cut(s) 784
Ppu21I YACGTR 1 cut(s) 606
PscI ACATGT 1 cut(s) 763
Psp124BI GAGCTC 1 cut(s) 841
Psp6I CCWGG 4 cut(s) 129, 391, 793, 1039
PspCI CACGTG 1 cut(s) 606
PspGI CCWGG 4 cut(s) 129, 391, 793, 1039
PspN4I GGNNCC 3 cut(s) 616, 679, 1038
PspPI GGNCC 2 cut(s) 482, 614
PstI CTGCAG 1 cut(s) 16
PsuI RGATCY 2 cut(s) 323, 965
RsaI GTAC 3 cut(s) 262, 404, 755
RsaNI GTAC 3 cut(s) 261, 403, 754
RseI CAYNNNNRTG 1 cut(s) 604
SacI GAGCTC 1 cut(s) 841
SapI GCTCTTC 1 cut(s) 1053
SaqAI TTAA 2 cut(s) 363, 542
SatI GCNGC 5 cut(s) 15, 534, 555, 591, 855
Sau3AI GATC 4 cut(s) 323, 758, 877, 965
Sau96I GGNCC 2 cut(s) 482, 614
SchI GAGTC 1 cut(s) 784
ScrFI CCNGG 5 cut(s) 131, 393, 795, 1004, 1041
SduI GDGCHC 4 cut(s) 49, 691, 841, 1048
SfcI CTRYAG 2 cut(s) 12, 168
SinI GGWCC 2 cut(s) 482, 614
SmiMI CAYNNNNRTG 1 cut(s) 604
SphI GCATGC 1 cut(s) 533
SsiI CCGC 4 cut(s) 591, 829, 852, 1055
SspMI CTAG 2 cut(s) 645, 657
SstI GAGCTC 1 cut(s) 841
StyD4I CCNGG 5 cut(s) 129, 391, 793, 1002, 1039
TaaI ACNGT 3 cut(s) 112, 310, 356
TaiI ACGT 2 cut(s) 565, 608
TaqI TCGA 3 cut(s) 673, 804, 1032
TauI GCSGC 1 cut(s) 593
TfiI GAWTC 2 cut(s) 641, 746
Tru1I TTAA 2 cut(s) 363, 542
Tru9I TTAA 2 cut(s) 363, 542
TscAI CASTG 2 cut(s) 313, 439
TseFI GTSAC 1 cut(s) 787
TseI GCWGC 4 cut(s) 14, 533, 554, 854
Tsp45I GTSAC 1 cut(s) 787
TspDTI ATGAA 2 cut(s) 258, 454
TspGWI ACGGA 1 cut(s) 316
TspRI CASTG 2 cut(s) 313, 439
VpaK11BI GGWCC 2 cut(s) 482, 614
XapI RAATTY 5 cut(s) 73, 203, 214, 449, 899
XceI RCATGY 2 cut(s) 533, 767
XcmI CCANNNNNNNNNTGG 2 cut(s) 151, 439
XspI CTAG 2 cut(s) 645, 657
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.