AT1G74730

Protein of unknown function (DUF1118)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0014162
Physical Location & Seq
Forward (+)
28078852 .. 28079987
1136 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G74730.1

Sequence Viewer

Length: 597 bp
ATGGCTGTCGTCGGCGCTCCAATATCGTCTCCGGCGGCTCAGCTGCAGACACAATTTCTCTCCAATCCCATTCTCCCCCGCTTTCGCCGGTCTTTCTCCACCGGAAAATCACCAGCAACTTTCTCCGTCGTAGCTATGGCTCCCCAGAAAAAGGTGAACAAATATGATGCCAAGTGGAAGAAACAATGGTACGGAGCTGGATTGTTTTTCGAAGGGAGTGAGCAAATAAACGTTGATGTTTTCAAGAAGCTGGAGAAGCGAAAAGTGTTGAGCAACGTTGAGAAATCTGGCCTGCTGTCAAAAGCAGAGGGGTTGGGACTCACATTGTCATCTCTTGAGAAGCTTAAAGTCTTCTCCAAAGCAGAGGACCTTGGTCTTCTCAGTCTCCTTGAGAACTTAGCTGGAACATCGCCTGCGGTCTTAGCCTCGGCTGCATTACCAGCTCTCACGGCTGCTATTGTAGCCGTGGTGTTGATCCCGGATGACTCAACTACTCTAGTGGTTGCTCAGGCGGTTTTGGCCGGTGCTCTTGCGCTTACAGGGGTTGTTTTGTTGGTTGGTTCTGTTGTTTTGGATGGACTTCAAGAAGCTGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1118

Protein Analysis

198

Amino Acids

20.73

Weight (kDa)

9.3

Isoelectric Point (pI)

37.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1118 PF06549 83 - 197 1.6e-44 Protein of unknown function (DUF1118)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014162)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G74730
fragaria_vesca FvH4_5g15500
malus_domestica MD06G1188100.v1.1 MD14G1194200.v1.1
prunus_persica Prupe.5G186300_v2.0.a1
pyrus_communis pycom06g16690 pycom14g16160
rosa_chinensis RchiOBHm_Chr7g0180591
rosa_laevigata RLG00000005252
rosa_multiflora Rmu_sc0005690.1_g000019
rosa_roxburghii Rroxscaffold_3G00272760
rosa_rugosa Rorug06G0434000
rosa_samantha Rh7AG035400 Rh7BG035300 Rh7CG037000 Rh7DG035600
rosa_wichuraiana Rw7G002910

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 35, 79, 416, 512
AclI AACGTT 2 cut(s) 231, 276
AclWI GGATC 1 cut(s) 469
AcoI YGGCCR 1 cut(s) 519
AfaI GTAC 1 cut(s) 191
AfiI CCNNNNNNNGG 1 cut(s) 151
AgsI TTSAA 2 cut(s) 244, 584
AluBI AGCT 8 cut(s) 43, 134, 197, 250, 343, 401, 443, 590
AluI AGCT 8 cut(s) 43, 134, 197, 250, 343, 401, 443, 590
Alw21I GWGCWC 1 cut(s) 529
Alw26I GTCTC 2 cut(s) 33, 389
AlwI GGATC 1 cut(s) 469
AoxI GGCC 2 cut(s) 289, 519
ApeKI GCWGC 3 cut(s) 43, 431, 452
AspLEI GCGC 2 cut(s) 17, 535
AspS9I GGNCC 1 cut(s) 367
AsuC2I CCSGG 1 cut(s) 479
AsuHPI GGTGA 2 cut(s) 102, 166
AsuII TTCGAA 1 cut(s) 210
AvaII GGWCC 1 cut(s) 367
BbsI GAAGAC 2 cut(s) 343, 368
Bbv12I GWGCWC 1 cut(s) 529
BbvI GCAGC 3 cut(s) 30, 418, 439
BccI CCATC 1 cut(s) 569
BceAI ACGGC 2 cut(s) 449, 465
BcnI CCSGG 1 cut(s) 479
BcoDI GTCTC 2 cut(s) 33, 389
BfaI CTAG 1 cut(s) 497
BfmI CTRYAG 1 cut(s) 44
BfoI RGCGCY 1 cut(s) 18
BisI GCNGC 4 cut(s) 36, 44, 432, 453
BlpI GCTNAGC 1 cut(s) 39
BlsI GCNGC 4 cut(s) 37, 45, 433, 454
Bme1390I CCNGG 1 cut(s) 479
Bme18I GGWCC 1 cut(s) 367
BmgT120I GGNCC 1 cut(s) 367
BmiI GGNNCC 1 cut(s) 141
BmrFI CCNGG 1 cut(s) 479
BmsI GCATC 1 cut(s) 157
BoxI GACNNNNGTC 1 cut(s) 372
BpiI GAAGAC 2 cut(s) 343, 368
BpmI CTGGAG 1 cut(s) 272
Bpu10I CCTNAGC 1 cut(s) 507
Bpu1102I GCTNAGC 1 cut(s) 39
Bpu14I TTCGAA 1 cut(s) 210
BpuEI CTTGAG 2 cut(s) 356, 410
BpuMI CCSGG 1 cut(s) 479
BsaJI CCNNGG 3 cut(s) 370, 426, 465
BsaWI WCCGGW 1 cut(s) 101
Bsc4I CCNNNNNNNGG 1 cut(s) 151
Bse118I RCCGGY 2 cut(s) 87, 521
BseDI CCNNGG 3 cut(s) 370, 426, 465
BseGI GGATG 2 cut(s) 487, 580
BseLI CCNNNNNNNGG 1 cut(s) 151
BseMII CTCAG 3 cut(s) 53, 394, 521
BseXI GCAGC 3 cut(s) 30, 418, 439
BshFI GGCC 2 cut(s) 291, 521
BsiHKAI GWGCWC 1 cut(s) 529
BsiSI CCGG 5 cut(s) 32, 88, 102, 479, 522
BslFI GGGAC 1 cut(s) 330
BslI CCNNNNNNNGG 1 cut(s) 151
BsmAI GTCTC 2 cut(s) 33, 389
BsmBI CGTCTC 1 cut(s) 33
BsmFI GGGAC 1 cut(s) 330
BsnI GGCC 2 cut(s) 291, 521
Bsp119I TTCGAA 1 cut(s) 210
Bsp1286I GDGCHC 1 cut(s) 529
Bsp143I GATC 1 cut(s) 474
Bsp1720I GCTNAGC 1 cut(s) 39
BspACI CCGC 4 cut(s) 35, 79, 416, 512
BspANI GGCC 2 cut(s) 291, 521
BspCNI CTCAG 3 cut(s) 52, 393, 520
BspLI GGNNCC 1 cut(s) 141
BspMAI CTGCAG 1 cut(s) 48
BspPI GGATC 1 cut(s) 469
BspT104I TTCGAA 1 cut(s) 210
BsrFI RCCGGY 2 cut(s) 87, 521
BssAI RCCGGY 2 cut(s) 87, 521
BssECI CCNNGG 3 cut(s) 370, 426, 465
BssMI GATC 1 cut(s) 474
BssT1I CCWWGG 1 cut(s) 370
BstBI TTCGAA 1 cut(s) 210
BstC8I GCNNGC 2 cut(s) 293, 414
BstDEI CTNAG 5 cut(s) 39, 380, 397, 421, 507
BstDSI CCRYGG 1 cut(s) 465
BstF5I GGATG 2 cut(s) 487, 580
BstH2I RGCGCY 1 cut(s) 18
BstHHI GCGC 2 cut(s) 17, 535
BstKTI GATC 1 cut(s) 477
BstMAI GTCTC 2 cut(s) 33, 389
BstMBI GATC 1 cut(s) 474
BstMWI GCNNNNNNNGC 7 cut(s) 256, 422, 431, 440, 449, 461, 518
BstPAI GACNNNNGTC 1 cut(s) 372
BstSCI CCNGG 1 cut(s) 477
BstSFI CTRYAG 1 cut(s) 44
BstV1I GCAGC 3 cut(s) 30, 418, 439
BstV2I GAAGAC 2 cut(s) 343, 368
BsuRI GGCC 2 cut(s) 291, 521
BtgI CCRYGG 1 cut(s) 465
BtgZI GCGATG 1 cut(s) 393
BtsCI GGATG 2 cut(s) 487, 580
Cac8I GCNNGC 2 cut(s) 293, 414
CfoI GCGC 2 cut(s) 17, 535
Cfr10I RCCGGY 2 cut(s) 87, 521
Cfr13I GGNCC 1 cut(s) 367
Csp6I GTAC 1 cut(s) 190
CviQI GTAC 1 cut(s) 190
DdeI CTNAG 5 cut(s) 39, 380, 397, 421, 507
DpnI GATC 1 cut(s) 476
DpnII GATC 1 cut(s) 474
EaeI YGGCCR 1 cut(s) 519
Eco130I CCWWGG 1 cut(s) 370
Eco47I GGWCC 1 cut(s) 367
EcoO109I RGGNCCY 1 cut(s) 367
EcoT14I CCWWGG 1 cut(s) 370
ErhI CCWWGG 1 cut(s) 370
Esp3I CGTCTC 1 cut(s) 33
FaiI YATR 2 cut(s) 137, 165
FaqI GGGAC 1 cut(s) 330
FauI CCCGC 1 cut(s) 86
Fnu4HI GCNGC 4 cut(s) 36, 44, 432, 453
FokI GGATG 2 cut(s) 494, 587
Fsp4HI GCNGC 4 cut(s) 36, 44, 432, 453
FspBI CTAG 1 cut(s) 497
GlaI GCGC 2 cut(s) 16, 534
GluI GCNGC 4 cut(s) 36, 44, 432, 453
GsuI CTGGAG 1 cut(s) 272
HaeII RGCGCY 1 cut(s) 18
HaeIII GGCC 2 cut(s) 291, 521
HapII CCGG 5 cut(s) 32, 88, 102, 479, 522
HhaI GCGC 2 cut(s) 17, 535
Hin6I GCGC 2 cut(s) 15, 533
HinP1I GCGC 2 cut(s) 15, 533
HindIII AAGCTT 1 cut(s) 341
HinfI GANTC 2 cut(s) 318, 485
HpaII CCGG 5 cut(s) 32, 88, 102, 479, 522
HphI GGTGA 2 cut(s) 102, 166
Hpy166II GTNNAC 1 cut(s) 157
Hpy188III TCNNGA 3 cut(s) 244, 335, 584
Hpy8I GTNNAC 1 cut(s) 157
Hpy99I CGWCG 2 cut(s) 14, 131
HpyAV CCTTC 1 cut(s) 206
HpyCH4IV ACGT 2 cut(s) 231, 276
HpyCH4V TGCA 2 cut(s) 46, 434
HpyF10VI GCNNNNNNNGC 7 cut(s) 256, 422, 431, 440, 449, 461, 518
HpyF3I CTNAG 5 cut(s) 39, 380, 397, 421, 507
HpySE526I ACGT 2 cut(s) 231, 276
HspAI GCGC 2 cut(s) 15, 533
Kzo9I GATC 1 cut(s) 474
LmnI GCTCC 3 cut(s) 22, 145, 194
Lsp1109I GCAGC 3 cut(s) 30, 418, 439
LweI GCATC 1 cut(s) 157
MaeI CTAG 1 cut(s) 497
MaeII ACGT 2 cut(s) 231, 276
MalI GATC 1 cut(s) 476
MboI GATC 1 cut(s) 474
MboII GAAGA 3 cut(s) 190, 343, 368
MhlI GDGCHC 1 cut(s) 529
MluCI AATT 1 cut(s) 53
MlyI GAGTC 2 cut(s) 312, 479
MnlI CCTC 3 cut(s) 301, 358, 436
MseI TTAA 1 cut(s) 345
MspA1I CMGCKG 1 cut(s) 43
MspI CCGG 5 cut(s) 32, 88, 102, 479, 522
MspR9I CCNGG 1 cut(s) 479
MwoI GCNNNNNNNGC 7 cut(s) 256, 422, 431, 440, 449, 461, 518
NciI CCSGG 1 cut(s) 479
NdeII GATC 1 cut(s) 474
NlaIV GGNNCC 1 cut(s) 141
NmeAIII GCCGAG 1 cut(s) 407
NspV TTCGAA 1 cut(s) 210
PfoI TCCNGGA 1 cut(s) 477
PkrI GCNGC 4 cut(s) 37, 45, 433, 454
PleI GAGTC 2 cut(s) 312, 479
PpsI GAGTC 2 cut(s) 312, 479
PpuMI RGGWCCY 1 cut(s) 367
PshAI GACNNNNGTC 1 cut(s) 372
Psp1406I AACGTT 2 cut(s) 231, 276
Psp5II RGGWCCY 1 cut(s) 367
PspN4I GGNNCC 1 cut(s) 141
PspPI GGNCC 1 cut(s) 367
PspPPI RGGWCCY 1 cut(s) 367
PstI CTGCAG 1 cut(s) 48
PvuII CAGCTG 1 cut(s) 43
RsaI GTAC 1 cut(s) 191
RsaNI GTAC 1 cut(s) 190
SaqAI TTAA 1 cut(s) 345
SatI GCNGC 4 cut(s) 36, 44, 432, 453
Sau3AI GATC 1 cut(s) 474
Sau96I GGNCC 1 cut(s) 367
SchI GAGTC 2 cut(s) 312, 479
ScrFI CCNGG 1 cut(s) 479
SduI GDGCHC 1 cut(s) 529
SfaNI GCATC 1 cut(s) 157
SfcI CTRYAG 1 cut(s) 44
SfuI TTCGAA 1 cut(s) 210
SinI GGWCC 1 cut(s) 367
SmlI CTYRAG 2 cut(s) 335, 389
SmoI CTYRAG 2 cut(s) 335, 389
Sse9I AATT 1 cut(s) 53
SsiI CCGC 4 cut(s) 35, 79, 416, 512
SspMI CTAG 1 cut(s) 497
StyD4I CCNGG 1 cut(s) 477
StyI CCWWGG 1 cut(s) 370
TaiI ACGT 2 cut(s) 234, 279
TaqI TCGA 1 cut(s) 210
TasI AATT 1 cut(s) 53
TauI GCSGC 1 cut(s) 38
Tru1I TTAA 1 cut(s) 345
Tru9I TTAA 1 cut(s) 345
TseI GCWGC 3 cut(s) 43, 431, 452
TspGWI ACGGA 2 cut(s) 115, 207
VpaK11BI GGWCC 1 cut(s) 367
XspI CTAG 1 cut(s) 497
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.