MD14G1194200.v1.1

Protein of unknown function (DUF1118)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0014162
Physical Location & Seq
Reverse (-)
28542687 .. 28543780
1094 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1194200.v1.1.491

Sequence Viewer

Length: 606 bp
ATGGCGGTCACTGCTGCAGCATCTTCAGCTACCTGCAATCTCAACCACACCAGAAACTTCCTCTCGTCCAAACCCCACTTCAATTCCATCTCTGGCGCTCCCCGCACCTTCCCCGCCAAATCTCGATCATCAACAATCGTTTCCATGGCGCCCCAAAAGAAGGTGAACAAGCTCGACAGCGGCTGGGAGAAAAAGTGGTACGGAGCGGGAATCTTCTACGAAGGGGCGGAGGAAGTGGAAGTCGACGTCTTCAAGAAGCTGGAGAAGCGGAAGGTCCTCAGCAACGTCGAGAAGGCGGGGCTTTTGTCCAAGGCGGAGGAGCTCGGGGTCACCCTGTCGTCGATTGAAAAATTGGGGCTGTTTTCCAAGGCGGAGGAGCTCGGGCTGCTCAGCCTGCTCGAGAAGGTGGCTGGATTCTCCCCCGCTGCGTTGGCCTCGGCGGCTCTGCCCATTTTGGTGGCAGCTGTGGTGGCAGTCGTGGTTATACCGGATGACTCGGCAGCGCTGGTGGCTGCGCAGGCTGTGGTGGCTGGTGTGCTTGGGGCCGGCGCTGCTGGTTTGTTAGTTGGGTCAGTTGTGTTGGATGGTTTGCAAGAAGCTGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1118

Protein Analysis

202

Amino Acids

20.72

Weight (kDa)

6.75

Isoelectric Point (pI)

33.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1118 PF06549 86 - 200 2.7e-48 Protein of unknown function (DUF1118)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014162)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G74730
fragaria_vesca FvH4_5g15500
malus_domestica MD06G1188100.v1.1 MD14G1194200.v1.1
prunus_persica Prupe.5G186300_v2.0.a1
pyrus_communis pycom06g16690 pycom14g16160
rosa_chinensis RchiOBHm_Chr7g0180591
rosa_laevigata RLG00000005252
rosa_multiflora Rmu_sc0005690.1_g000019
rosa_roxburghii Rroxscaffold_3G00272760
rosa_rugosa Rorug06G0434000
rosa_samantha Rh7AG035400 Rh7BG035300 Rh7CG037000 Rh7DG035600
rosa_wichuraiana Rw7G002910

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 249
Acc16I TGCGCA 1 cut(s) 516
Acc36I ACCTGC 1 cut(s) 41
AccB1I GGYRCC 1 cut(s) 148
AccBSI CCGCTC 1 cut(s) 206
AccI GTMKAC 1 cut(s) 243
AcuI CTGAAG 1 cut(s) 9
AcyI GRCGYC 2 cut(s) 149, 246
AfaI GTAC 1 cut(s) 200
AfeI AGCGCT 1 cut(s) 504
AfiI CCNNNNNNNGG 1 cut(s) 160
AgsI TTSAA 3 cut(s) 82, 253, 347
AjuI GAANNNNNNNTTGG 2 cut(s) 62, 94
AluBI AGCT 7 cut(s) 29, 172, 259, 322, 379, 464, 599
AluI AGCT 7 cut(s) 29, 172, 259, 322, 379, 464, 599
Alw21I GWGCWC 2 cut(s) 324, 381
AlwNI CAGNNNCTG 1 cut(s) 183
Ama87I CYCGRG 3 cut(s) 323, 380, 398
Aor51HI AGCGCT 1 cut(s) 504
AoxI GGCC 2 cut(s) 432, 543
ApeKI GCWGC 8 cut(s) 14, 17, 385, 425, 461, 500, 512, 551
AspLEI GCGC 5 cut(s) 98, 151, 505, 517, 551
AspS9I GGNCC 2 cut(s) 274, 543
AsuHPI GGTGA 2 cut(s) 175, 322
AvaI CYCGRG 3 cut(s) 323, 380, 398
AvaII GGWCC 1 cut(s) 274
BanI GGYRCC 1 cut(s) 148
BanII GRGCYC 2 cut(s) 324, 381
BbsI GAAGAC 1 cut(s) 241
Bbv12I GWGCWC 2 cut(s) 324, 381
BbvCI CCTCAGC 1 cut(s) 278
BbvI GCAGC 7 cut(s) 29, 372, 412, 473, 499, 512, 538
BccI CCATC 2 cut(s) 95, 578
BfmI CTRYAG 1 cut(s) 15
BfoI RGCGCY 4 cut(s) 99, 152, 506, 552
BfuAI ACCTGC 1 cut(s) 41
BglI GCCNNNNNGGC 1 cut(s) 440
BlpI GCTNAGC 1 cut(s) 389
Bme18I GGWCC 1 cut(s) 274
BmeT110I CYCGRG 3 cut(s) 323, 380, 398
BmgT120I GGNCC 2 cut(s) 274, 543
BmiI GGNNCC 2 cut(s) 150, 544
BmsI GCATC 1 cut(s) 29
BpiI GAAGAC 1 cut(s) 241
BpmI CTGGAG 1 cut(s) 281
Bpu10I CCTNAGC 1 cut(s) 278
Bpu1102I GCTNAGC 1 cut(s) 389
BsaHI GRCGYC 2 cut(s) 149, 246
BsaJI CCNNGG 4 cut(s) 144, 309, 366, 435
BsaWI WCCGGW 1 cut(s) 487
BsaXI ACNNNNNCTCC 2 cut(s) 179, 209
Bsc4I CCNNNNNNNGG 1 cut(s) 160
Bse118I RCCGGY 1 cut(s) 545
BseDI CCNNGG 4 cut(s) 144, 309, 366, 435
BseGI GGATG 2 cut(s) 496, 589
BseLI CCNNNNNNNGG 1 cut(s) 160
BseMII CTCAG 2 cut(s) 292, 403
BseRI GAGGAG 2 cut(s) 332, 389
BseXI GCAGC 7 cut(s) 29, 372, 412, 473, 499, 512, 538
BseYI CCCAGC 1 cut(s) 183
BshFI GGCC 2 cut(s) 434, 545
BshNI GGYRCC 1 cut(s) 148
BsiHKAI GWGCWC 2 cut(s) 324, 381
BsiHKCI CYCGRG 3 cut(s) 323, 380, 398
BsiSI CCGG 2 cut(s) 488, 546
BslI CCNNNNNNNGG 1 cut(s) 160
BsnI GGCC 2 cut(s) 434, 545
BsoBI CYCGRG 3 cut(s) 323, 380, 398
Bsp1286I GDGCHC 2 cut(s) 324, 381
Bsp143I GATC 1 cut(s) 125
Bsp1720I GCTNAGC 1 cut(s) 389
Bsp19I CCATGG 1 cut(s) 144
BspANI GGCC 2 cut(s) 434, 545
BspCNI CTCAG 2 cut(s) 291, 402
BspLI GGNNCC 2 cut(s) 150, 544
BspMAI CTGCAG 1 cut(s) 19
BspMI ACCTGC 1 cut(s) 41
BspT107I GGYRCC 1 cut(s) 148
BsrBI CCGCTC 1 cut(s) 206
BsrFI RCCGGY 1 cut(s) 545
BssAI RCCGGY 1 cut(s) 545
BssECI CCNNGG 4 cut(s) 144, 309, 366, 435
BssMI GATC 1 cut(s) 125
BssNI GRCGYC 2 cut(s) 149, 246
BssT1I CCWWGG 3 cut(s) 144, 309, 366
BstACI GRCGYC 2 cut(s) 149, 246
BstC8I GCNNGC 3 cut(s) 395, 519, 547
BstDEI CTNAG 2 cut(s) 278, 389
BstDSI CCRYGG 1 cut(s) 144
BstEII GGTNACC 1 cut(s) 328
BstF5I GGATG 2 cut(s) 496, 589
BstH2I RGCGCY 4 cut(s) 99, 152, 506, 552
BstHHI GCGC 5 cut(s) 98, 151, 505, 517, 551
BstKTI GATC 1 cut(s) 128
BstMBI GATC 1 cut(s) 125
BstPI GGTNACC 1 cut(s) 328
BstSFI CTRYAG 1 cut(s) 15
BstV1I GCAGC 7 cut(s) 29, 372, 412, 473, 499, 512, 538
BstV2I GAAGAC 1 cut(s) 241
BstXI CCANNNNNNTGG 1 cut(s) 457
BsuRI GGCC 2 cut(s) 434, 545
BtgI CCRYGG 1 cut(s) 144
BtsCI GGATG 2 cut(s) 496, 589
BtsI GCAGTG 1 cut(s) 9
BtsIMutI CAGTG 1 cut(s) 9
BveI ACCTGC 1 cut(s) 41
Cac8I GCNNGC 3 cut(s) 395, 519, 547
CaiI CAGNNNCTG 1 cut(s) 183
CfoI GCGC 5 cut(s) 98, 151, 505, 517, 551
Cfr10I RCCGGY 1 cut(s) 545
Cfr13I GGNCC 2 cut(s) 274, 543
Csp6I GTAC 1 cut(s) 199
CviAII CATG 1 cut(s) 145
CviQI GTAC 1 cut(s) 199
DdeI CTNAG 2 cut(s) 278, 389
DinI GGCGCC 1 cut(s) 150
DpnI GATC 1 cut(s) 127
DpnII GATC 1 cut(s) 125
EciI GGCGGA 3 cut(s) 242, 329, 386
Ecl136II GAGCTC 2 cut(s) 322, 379
Eco130I CCWWGG 3 cut(s) 144, 309, 366
Eco24I GRGCYC 2 cut(s) 324, 381
Eco47I GGWCC 1 cut(s) 274
Eco47III AGCGCT 1 cut(s) 504
Eco53kI GAGCTC 2 cut(s) 322, 379
Eco57I CTGAAG 1 cut(s) 9
Eco88I CYCGRG 3 cut(s) 323, 380, 398
Eco91I GGTNACC 1 cut(s) 328
EcoICRI GAGCTC 2 cut(s) 322, 379
EcoO109I RGGNCCY 1 cut(s) 274
EcoO65I GGTNACC 1 cut(s) 328
EcoT14I CCWWGG 3 cut(s) 144, 309, 366
EcoT38I GRGCYC 2 cut(s) 324, 381
EgeI GGCGCC 1 cut(s) 150
EheI GGCGCC 1 cut(s) 150
ErhI CCWWGG 3 cut(s) 144, 309, 366
FaeI CATG 1 cut(s) 148
FaiI YATR 2 cut(s) 146, 485
FatI CATG 1 cut(s) 144
FauI CCCGC 5 cut(s) 110, 121, 199, 289, 430
FblI GTMKAC 1 cut(s) 243
FokI GGATG 2 cut(s) 503, 596
FriOI GRGCYC 2 cut(s) 324, 381
FspI TGCGCA 1 cut(s) 516
GlaI GCGC 5 cut(s) 97, 150, 504, 516, 550
GsaI CCCAGC 1 cut(s) 187
GsuI CTGGAG 1 cut(s) 281
HaeII RGCGCY 4 cut(s) 99, 152, 506, 552
HaeIII GGCC 2 cut(s) 434, 545
HapII CCGG 2 cut(s) 488, 546
HhaI GCGC 5 cut(s) 98, 151, 505, 517, 551
Hin1I GRCGYC 2 cut(s) 149, 246
Hin1II CATG 1 cut(s) 148
Hin6I GCGC 5 cut(s) 96, 149, 503, 515, 549
HinP1I GCGC 5 cut(s) 96, 149, 503, 515, 549
HincII GTYRAC 1 cut(s) 244
HindII GTYRAC 1 cut(s) 244
HinfI GANTC 3 cut(s) 210, 414, 494
HpaII CCGG 2 cut(s) 488, 546
HphI GGTGA 2 cut(s) 175, 322
Hpy166II GTNNAC 2 cut(s) 166, 244
Hpy188III TCNNGA 4 cut(s) 123, 253, 289, 400
Hpy8I GTNNAC 2 cut(s) 166, 244
Hpy99I CGWCG 3 cut(s) 248, 290, 343
HpyAV CCTTC 6 cut(s) 118, 154, 215, 265, 286, 397
HpyCH4IV ACGT 2 cut(s) 246, 285
HpyCH4V TGCA 3 cut(s) 17, 36, 592
HpyF3I CTNAG 2 cut(s) 278, 389
HpySE526I ACGT 2 cut(s) 246, 285
Hsp92I GRCGYC 2 cut(s) 149, 246
Hsp92II CATG 1 cut(s) 148
HspAI GCGC 5 cut(s) 96, 149, 503, 515, 549
KasI GGCGCC 1 cut(s) 148
KroI GCCGGC 1 cut(s) 545
KroNI GCCGGC 1 cut(s) 547
Kzo9I GATC 1 cut(s) 125
LmnI GCTCC 4 cut(s) 103, 203, 319, 376
Lsp1109I GCAGC 7 cut(s) 29, 372, 412, 473, 499, 512, 538
LweI GCATC 1 cut(s) 29
MaeII ACGT 2 cut(s) 246, 285
MaeIII GTNAC 2 cut(s) 7, 328
MalI GATC 1 cut(s) 127
MbiI CCGCTC 1 cut(s) 206
MboI GATC 1 cut(s) 125
MboII GAAGA 3 cut(s) 15, 205, 241
MhlI GDGCHC 2 cut(s) 324, 381
MluCI AATT 2 cut(s) 82, 350
Mly113I GGCGCC 1 cut(s) 149
MlyI GAGTC 1 cut(s) 488
MmeI TCCRAC 1 cut(s) 561
MnlI CCTC 6 cut(s) 71, 223, 287, 310, 367, 445
MroNI GCCGGC 1 cut(s) 545
MseI TTAA 1 cut(s) 604
MslI CAYNNNNRTG 1 cut(s) 455
MspA1I CMGCKG 3 cut(s) 180, 425, 464
MspI CCGG 2 cut(s) 488, 546
NaeI GCCGGC 1 cut(s) 547
NarI GGCGCC 1 cut(s) 149
NcoI CCATGG 1 cut(s) 144
NdeII GATC 1 cut(s) 125
NgoMIV GCCGGC 1 cut(s) 545
NlaIII CATG 1 cut(s) 148
NlaIV GGNNCC 2 cut(s) 150, 544
NmeAIII GCCGAG 2 cut(s) 416, 476
NmuCI GTSAC 2 cut(s) 7, 328
NsbI TGCGCA 1 cut(s) 516
PaeR7I CTCGAG 1 cut(s) 398
PdiI GCCGGC 1 cut(s) 547
PfeI GAWTC 2 cut(s) 210, 414
PleI GAGTC 1 cut(s) 488
PluTI GGCGCC 1 cut(s) 152
PpsI GAGTC 1 cut(s) 488
PpuMI RGGWCCY 1 cut(s) 274
Psp124BI GAGCTC 2 cut(s) 324, 381
Psp5II RGGWCCY 1 cut(s) 274
PspEI GGTNACC 1 cut(s) 328
PspFI CCCAGC 1 cut(s) 183
PspN4I GGNNCC 2 cut(s) 150, 544
PspPI GGNCC 2 cut(s) 274, 543
PspPPI RGGWCCY 1 cut(s) 274
PstI CTGCAG 1 cut(s) 19
PstNI CAGNNNCTG 1 cut(s) 183
PvuII CAGCTG 1 cut(s) 464
RsaI GTAC 1 cut(s) 200
RsaNI GTAC 1 cut(s) 199
RseI CAYNNNNRTG 1 cut(s) 455
SacI GAGCTC 2 cut(s) 324, 381
SalI GTCGAC 1 cut(s) 242
SaqAI TTAA 1 cut(s) 604
Sau3AI GATC 1 cut(s) 125
Sau96I GGNCC 2 cut(s) 274, 543
SchI GAGTC 1 cut(s) 488
SduI GDGCHC 2 cut(s) 324, 381
SfaNI GCATC 1 cut(s) 29
SfcI CTRYAG 1 cut(s) 15
SfoI GGCGCC 1 cut(s) 150
Sfr274I CTCGAG 1 cut(s) 398
SinI GGWCC 1 cut(s) 274
SlaI CTCGAG 1 cut(s) 398
SmiMI CAYNNNNRTG 1 cut(s) 455
SmlI CTYRAG 1 cut(s) 398
SmoI CTYRAG 1 cut(s) 398
Sse9I AATT 2 cut(s) 82, 350
SspDI GGCGCC 1 cut(s) 148
SstI GAGCTC 2 cut(s) 324, 381
StyI CCWWGG 3 cut(s) 144, 309, 366
TaiI ACGT 2 cut(s) 249, 288
TaqI TCGA 6 cut(s) 124, 174, 243, 288, 341, 399
TasI AATT 2 cut(s) 82, 350
TauI GCSGC 2 cut(s) 183, 443
TfiI GAWTC 2 cut(s) 210, 414
Tru1I TTAA 1 cut(s) 604
Tru9I TTAA 1 cut(s) 604
TscAI CASTG 1 cut(s) 16
TseFI GTSAC 2 cut(s) 7, 328
TseI GCWGC 8 cut(s) 14, 17, 385, 425, 461, 500, 512, 551
Tsp45I GTSAC 2 cut(s) 7, 328
TspGWI ACGGA 1 cut(s) 216
TspRI CASTG 1 cut(s) 16
VpaK11BI GGWCC 1 cut(s) 274
XhoI CTCGAG 1 cut(s) 398
XmiI GTMKAC 1 cut(s) 243
ZraI GACGTC 1 cut(s) 247
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.