MD06G1188100.v1.1

Protein of unknown function (DUF1118)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0014162
Physical Location & Seq
Reverse (-)
32529803 .. 32530821
1019 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1188100.v1.1.491

Sequence Viewer

Length: 606 bp
ATGGCGGTCAGTACTGCAGCATCTTCAGCTACCTGCAATCTCAACCACACCAGCAACTTCCTCTCCTCCAAACCCCACTTCAGTTTCATTTCTGGTTCATCCCGCAACTTCCCCGCCAAATCAAGGCCCTCAACAATCGTTTCCATGGCGCCCCAAAAGAAGGTGAACAAGCTCGACGGCGGCTGGGAGAAGAAGTGGTACGGAGCGGGAATCTTCTACGAAGGGGCGGAGGAAGTGGAAGTCGACGTCTTCAAGAAGCTGGAGAAGCGGAAGGTCCTCAGCAACGTCGAGAAGGCGGGGCTTTTGTCCAAGGCGGAGCAGCTCGGGGTGACCCTGTCGTCGATTGAGAAATTGGGGCTGTTTTCGAAGGCGGAGGAACTCGGGCTCCTCAGCCTGCTCGAGAAGGTGGCTGGATTCTCCCCAGCCGCCTTGGCCTCGGCGTCGCTGCCCATATTGGTGGCGGCTGTGGTGGCGGTCGTGGTTATACCGGATGACTCGACGGCCCTGGTGGCGGTGCAGGCTGTGGTGGCGGGCGTGCTTGGGGCTGGCGCTGCTGGATTGTTAGTTGGGTCAGTTGTCTTGGATGGCTTGCAAGAAGCTGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1118

Protein Analysis

202

Amino Acids

20.77

Weight (kDa)

6.75

Isoelectric Point (pI)

32.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1118 PF06549 86 - 200 7.1e-49 Protein of unknown function (DUF1118)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014162)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G74730
fragaria_vesca FvH4_5g15500
malus_domestica MD06G1188100.v1.1 MD14G1194200.v1.1
prunus_persica Prupe.5G186300_v2.0.a1
pyrus_communis pycom06g16690 pycom14g16160
rosa_chinensis RchiOBHm_Chr7g0180591
rosa_laevigata RLG00000005252
rosa_multiflora Rmu_sc0005690.1_g000019
rosa_roxburghii Rroxscaffold_3G00272760
rosa_rugosa Rorug06G0434000
rosa_samantha Rh7AG035400 Rh7BG035300 Rh7CG037000 Rh7DG035600
rosa_wichuraiana Rw7G002910

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 337
AatII GACGTC 1 cut(s) 249
Acc36I ACCTGC 1 cut(s) 41
AccB1I GGYRCC 1 cut(s) 148
AccBSI CCGCTC 1 cut(s) 206
AccI GTMKAC 1 cut(s) 243
AcuI CTGAAG 2 cut(s) 9, 64
AcyI GRCGYC 3 cut(s) 149, 246, 440
AfaI GTAC 2 cut(s) 13, 200
AfiI CCNNNNNNNGG 3 cut(s) 123, 160, 511
AgsI TTSAA 1 cut(s) 253
AjnI CCWGG 1 cut(s) 504
AjuI GAANNNNNNNTTGG 2 cut(s) 62, 94
AloI GAACNNNNNNTCC 2 cut(s) 369, 401
AluBI AGCT 5 cut(s) 29, 172, 259, 322, 599
AluI AGCT 5 cut(s) 29, 172, 259, 322, 599
Ama87I CYCGRG 3 cut(s) 323, 380, 398
AoxI GGCC 3 cut(s) 125, 432, 501
ApeKI GCWGC 4 cut(s) 17, 319, 445, 551
AspLEI GCGC 2 cut(s) 151, 551
AspS9I GGNCC 3 cut(s) 126, 274, 502
AsuHPI GGTGA 2 cut(s) 175, 340
AsuII TTCGAA 1 cut(s) 365
AvaI CYCGRG 3 cut(s) 323, 380, 398
AvaII GGWCC 1 cut(s) 274
BanI GGYRCC 1 cut(s) 148
BanII GRGCYC 1 cut(s) 387
BarI GAAGNNNNNNTAC 2 cut(s) 182, 214
BbsI GAAGAC 1 cut(s) 241
BbvCI CCTCAGC 2 cut(s) 278, 389
BbvI GCAGC 4 cut(s) 29, 331, 432, 538
BccI CCATC 1 cut(s) 578
BceAI ACGGC 2 cut(s) 193, 516
BciT130I CCWGG 1 cut(s) 506
BfmI CTRYAG 1 cut(s) 15
BfoI RGCGCY 2 cut(s) 152, 552
BfuAI ACCTGC 1 cut(s) 41
BglI GCCNNNNNGGC 2 cut(s) 431, 509
BisI GCNGC 7 cut(s) 18, 181, 320, 426, 446, 462, 552
BlsI GCNGC 7 cut(s) 19, 182, 321, 427, 447, 463, 553
BmcAI AGTACT 1 cut(s) 13
Bme1390I CCNGG 1 cut(s) 506
Bme18I GGWCC 1 cut(s) 274
BmeT110I CYCGRG 3 cut(s) 323, 380, 398
BmgT120I GGNCC 3 cut(s) 126, 274, 502
BmiI GGNNCC 2 cut(s) 150, 386
BmrFI CCNGG 1 cut(s) 506
BmsI GCATC 1 cut(s) 29
BpiI GAAGAC 1 cut(s) 241
BpmI CTGGAG 1 cut(s) 281
Bpu10I CCTNAGC 2 cut(s) 278, 389
Bpu14I TTCGAA 1 cut(s) 365
BsaHI GRCGYC 3 cut(s) 149, 246, 440
BsaJI CCNNGG 5 cut(s) 144, 309, 429, 435, 504
BsaWI WCCGGW 1 cut(s) 487
BsaXI ACNNNNNCTCC 6 cut(s) 47, 77, 179, 209, 369, 399
Bsc4I CCNNNNNNNGG 3 cut(s) 123, 160, 511
BseBI CCWGG 1 cut(s) 506
BseDI CCNNGG 5 cut(s) 144, 309, 429, 435, 504
BseGI GGATG 3 cut(s) 98, 496, 589
BseLI CCNNNNNNNGG 3 cut(s) 123, 160, 511
BseMII CTCAG 2 cut(s) 292, 403
BseRI GAGGAG 2 cut(s) 55, 377
BseXI GCAGC 4 cut(s) 29, 331, 432, 538
BseYI CCCAGC 2 cut(s) 183, 421
BsgI GTGCAG 1 cut(s) 536
Bsh1285I CGRYCG 1 cut(s) 477
BshFI GGCC 3 cut(s) 127, 434, 503
BshNI GGYRCC 1 cut(s) 148
BsiEI CGRYCG 1 cut(s) 477
BsiHKCI CYCGRG 3 cut(s) 323, 380, 398
BsiSI CCGG 1 cut(s) 488
BslI CCNNNNNNNGG 3 cut(s) 123, 160, 511
BsnI GGCC 3 cut(s) 127, 434, 503
BsoBI CYCGRG 3 cut(s) 323, 380, 398
Bsp119I TTCGAA 1 cut(s) 365
Bsp1286I GDGCHC 1 cut(s) 387
Bsp19I CCATGG 1 cut(s) 144
BspANI GGCC 3 cut(s) 127, 434, 503
BspCNI CTCAG 2 cut(s) 291, 402
BspLI GGNNCC 2 cut(s) 150, 386
BspMAI CTGCAG 1 cut(s) 19
BspMI ACCTGC 1 cut(s) 41
BspT104I TTCGAA 1 cut(s) 365
BspT107I GGYRCC 1 cut(s) 148
BsrBI CCGCTC 1 cut(s) 206
BssECI CCNNGG 5 cut(s) 144, 309, 429, 435, 504
BssNI GRCGYC 3 cut(s) 149, 246, 440
BssT1I CCWWGG 3 cut(s) 144, 309, 429
Bst2UI CCWGG 1 cut(s) 506
BstACI GRCGYC 3 cut(s) 149, 246, 440
BstBI TTCGAA 1 cut(s) 365
BstC8I GCNNGC 6 cut(s) 395, 519, 532, 536, 547, 590
BstDEI CTNAG 2 cut(s) 278, 389
BstDSI CCRYGG 1 cut(s) 144
BstEII GGTNACC 1 cut(s) 328
BstF5I GGATG 3 cut(s) 98, 496, 589
BstH2I RGCGCY 2 cut(s) 152, 552
BstHHI GCGC 2 cut(s) 151, 551
BstMCI CGRYCG 1 cut(s) 477
BstMWI GCNNNNNNNGC 8 cut(s) 26, 265, 431, 470, 509, 518, 527, 551
BstNI CCWGG 1 cut(s) 506
BstPI GGTNACC 1 cut(s) 328
BstSCI CCNGG 1 cut(s) 504
BstSFI CTRYAG 1 cut(s) 15
BstV1I GCAGC 4 cut(s) 29, 331, 432, 538
BstV2I GAAGAC 1 cut(s) 241
BstXI CCANNNNNNTGG 1 cut(s) 457
BsuRI GGCC 3 cut(s) 127, 434, 503
BtgI CCRYGG 1 cut(s) 144
BtsCI GGATG 3 cut(s) 98, 496, 589
BveI ACCTGC 1 cut(s) 41
Cac8I GCNNGC 6 cut(s) 395, 519, 532, 536, 547, 590
CfoI GCGC 2 cut(s) 151, 551
Cfr13I GGNCC 3 cut(s) 126, 274, 502
CseI GACGC 1 cut(s) 429
Csp6I GTAC 2 cut(s) 12, 199
CviAII CATG 1 cut(s) 145
CviQI GTAC 2 cut(s) 12, 199
DdeI CTNAG 2 cut(s) 278, 389
DinI GGCGCC 1 cut(s) 150
DrdI GACNNNNNNGTC 1 cut(s) 337
DseDI GACNNNNNNGTC 1 cut(s) 337
EciI GGCGGA 3 cut(s) 242, 329, 386
Eco130I CCWWGG 3 cut(s) 144, 309, 429
Eco24I GRGCYC 1 cut(s) 387
Eco47I GGWCC 1 cut(s) 274
Eco57I CTGAAG 2 cut(s) 9, 64
Eco88I CYCGRG 3 cut(s) 323, 380, 398
Eco91I GGTNACC 1 cut(s) 328
EcoO109I RGGNCCY 2 cut(s) 126, 274
EcoO65I GGTNACC 1 cut(s) 328
EcoRII CCWGG 1 cut(s) 504
EcoT14I CCWWGG 3 cut(s) 144, 309, 429
EcoT38I GRGCYC 1 cut(s) 387
EgeI GGCGCC 1 cut(s) 150
EheI GGCGCC 1 cut(s) 150
ErhI CCWWGG 3 cut(s) 144, 309, 429
FaeI CATG 1 cut(s) 148
FaiI YATR 3 cut(s) 146, 452, 485
FatI CATG 1 cut(s) 144
FauI CCCGC 5 cut(s) 110, 121, 199, 289, 523
FblI GTMKAC 1 cut(s) 243
Fnu4HI GCNGC 7 cut(s) 18, 181, 320, 426, 446, 462, 552
FokI GGATG 3 cut(s) 85, 503, 596
FriOI GRGCYC 1 cut(s) 387
Fsp4HI GCNGC 7 cut(s) 18, 181, 320, 426, 446, 462, 552
GlaI GCGC 2 cut(s) 150, 550
GluI GCNGC 7 cut(s) 18, 181, 320, 426, 446, 462, 552
GsaI CCCAGC 2 cut(s) 187, 425
GsuI CTGGAG 1 cut(s) 281
HaeII RGCGCY 2 cut(s) 152, 552
HaeIII GGCC 3 cut(s) 127, 434, 503
HapII CCGG 1 cut(s) 488
HgaI GACGC 1 cut(s) 429
HhaI GCGC 2 cut(s) 151, 551
Hin1I GRCGYC 3 cut(s) 149, 246, 440
Hin1II CATG 1 cut(s) 148
Hin6I GCGC 2 cut(s) 149, 549
HinP1I GCGC 2 cut(s) 149, 549
HincII GTYRAC 1 cut(s) 244
HindII GTYRAC 1 cut(s) 244
HinfI GANTC 3 cut(s) 210, 414, 494
HpaII CCGG 1 cut(s) 488
HphI GGTGA 2 cut(s) 175, 340
Hpy166II GTNNAC 2 cut(s) 166, 244
Hpy188III TCNNGA 3 cut(s) 253, 289, 400
Hpy8I GTNNAC 2 cut(s) 166, 244
Hpy99I CGWCG 6 cut(s) 179, 248, 290, 343, 445, 502
HpyAV CCTTC 6 cut(s) 154, 215, 265, 286, 361, 397
HpyCH4IV ACGT 2 cut(s) 246, 285
HpyCH4V TGCA 4 cut(s) 17, 36, 517, 592
HpyF10VI GCNNNNNNNGC 8 cut(s) 26, 265, 431, 470, 509, 518, 527, 551
HpyF3I CTNAG 2 cut(s) 278, 389
HpySE526I ACGT 2 cut(s) 246, 285
Hsp92I GRCGYC 3 cut(s) 149, 246, 440
Hsp92II CATG 1 cut(s) 148
HspAI GCGC 2 cut(s) 149, 549
KasI GGCGCC 1 cut(s) 148
LmnI GCTCC 3 cut(s) 203, 316, 390
Lsp1109I GCAGC 4 cut(s) 29, 331, 432, 538
LweI GCATC 1 cut(s) 29
MaeII ACGT 2 cut(s) 246, 285
MaeIII GTNAC 1 cut(s) 328
MbiI CCGCTC 1 cut(s) 206
MboII GAAGA 4 cut(s) 15, 202, 205, 241
MhlI GDGCHC 1 cut(s) 387
MluCI AATT 1 cut(s) 350
Mly113I GGCGCC 1 cut(s) 149
MlyI GAGTC 1 cut(s) 488
MnlI CCTC 8 cut(s) 71, 76, 139, 223, 287, 367, 398, 445
MseI TTAA 1 cut(s) 604
MslI CAYNNNNRTG 1 cut(s) 455
MspI CCGG 1 cut(s) 488
MspR9I CCNGG 1 cut(s) 506
MvaI CCWGG 1 cut(s) 506
MwoI GCNNNNNNNGC 8 cut(s) 26, 265, 431, 470, 509, 518, 527, 551
NarI GGCGCC 1 cut(s) 149
NcoI CCATGG 1 cut(s) 144
NlaIII CATG 1 cut(s) 148
NlaIV GGNNCC 2 cut(s) 150, 386
NmeAIII GCCGAG 1 cut(s) 416
NmuCI GTSAC 1 cut(s) 328
NspV TTCGAA 1 cut(s) 365
PaeR7I CTCGAG 1 cut(s) 398
PfeI GAWTC 2 cut(s) 210, 414
PflFI GACNNNGTC 1 cut(s) 334
PkrI GCNGC 7 cut(s) 19, 182, 321, 427, 447, 463, 553
PleI GAGTC 1 cut(s) 488
PluTI GGCGCC 1 cut(s) 152
PpsI GAGTC 1 cut(s) 488
PpuMI RGGWCCY 1 cut(s) 274
Psp5II RGGWCCY 1 cut(s) 274
Psp6I CCWGG 1 cut(s) 504
PspEI GGTNACC 1 cut(s) 328
PspFI CCCAGC 2 cut(s) 183, 421
PspGI CCWGG 1 cut(s) 504
PspN4I GGNNCC 2 cut(s) 150, 386
PspPI GGNCC 3 cut(s) 126, 274, 502
PspPPI RGGWCCY 1 cut(s) 274
PstI CTGCAG 1 cut(s) 19
PsyI GACNNNGTC 1 cut(s) 334
RsaI GTAC 2 cut(s) 13, 200
RsaNI GTAC 2 cut(s) 12, 199
RseI CAYNNNNRTG 1 cut(s) 455
SalI GTCGAC 1 cut(s) 242
SaqAI TTAA 1 cut(s) 604
SatI GCNGC 7 cut(s) 18, 181, 320, 426, 446, 462, 552
Sau96I GGNCC 3 cut(s) 126, 274, 502
ScaI AGTACT 1 cut(s) 13
SchI GAGTC 1 cut(s) 488
ScrFI CCNGG 1 cut(s) 506
SduI GDGCHC 1 cut(s) 387
SfaNI GCATC 1 cut(s) 29
SfcI CTRYAG 1 cut(s) 15
SfoI GGCGCC 1 cut(s) 150
Sfr274I CTCGAG 1 cut(s) 398
SfuI TTCGAA 1 cut(s) 365
SinI GGWCC 1 cut(s) 274
SlaI CTCGAG 1 cut(s) 398
SmiMI CAYNNNNRTG 1 cut(s) 455
SmlI CTYRAG 1 cut(s) 398
SmoI CTYRAG 1 cut(s) 398
Sse9I AATT 1 cut(s) 350
SspDI GGCGCC 1 cut(s) 148
StyD4I CCNGG 1 cut(s) 504
StyI CCWWGG 3 cut(s) 144, 309, 429
TaiI ACGT 2 cut(s) 249, 288
TaqI TCGA 7 cut(s) 174, 243, 288, 341, 365, 399, 497
TasI AATT 1 cut(s) 350
TatI WGTACW 1 cut(s) 11
TauI GCSGC 3 cut(s) 183, 428, 464
TfiI GAWTC 2 cut(s) 210, 414
Tru1I TTAA 1 cut(s) 604
Tru9I TTAA 1 cut(s) 604
TseFI GTSAC 1 cut(s) 328
TseI GCWGC 4 cut(s) 17, 319, 445, 551
Tsp45I GTSAC 1 cut(s) 328
TspDTI ATGAA 2 cut(s) 76, 87
TspGWI ACGGA 1 cut(s) 216
Tth111I GACNNNGTC 1 cut(s) 334
VpaK11BI GGWCC 1 cut(s) 274
XhoI CTCGAG 1 cut(s) 398
XmiI GTMKAC 1 cut(s) 243
ZraI GACGTC 1 cut(s) 247
ZrmI AGTACT 1 cut(s) 13
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.