AT1G77460

Protein kinase C conserved region 2 (CalB)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0009147
Physical Location & Seq
Forward (+)
29103608 .. 29111956
8349 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G77460.4

Sequence Viewer

Length: 6411 bp
ATGTTGAAGGCCTTTCTTCCTGGAACGCAGGAGGAGGAAACATTATCTTCTTTGCAGTCTGGGAAAGTCGATGCAAAAATGGAAATGGACGACCCAGAGAAGGCAATGGCTACAGTTGCTCAGCTCATAGAGCAACTTCATGCGAAAACATCTTCACCGCAAGATAAAGAACTCACAACAGCTCGTCTACTAGGTATTGCAAAAGGCAAAAGGGAGGCAAGAAGGCTCATCGGTTCGTATGGCCAAGCAATGCCTTTATTTATTTCCATGCTTAGAAATGGGACGACTTTGGCCAAAGTAAATGTCGCTTCAATCCTTTGTGTATTGTGCAAAGACAAAGACTTGCGACTAAAAGTGCTTCTAGGAGGATGCATCCCGCCATTACTCTCGGTTTTGAAGTCCGGAACCATGGAAACTAGAAAAGCAGCAGCAGAAGCTATATATGAGGTTTCTTCTGCTGGTATTTCCAATGACCACATTGGAATGAAAATATTTATCACAGAAGGTGTTGTTCCAACTTTGTGGGATCAACTTAGCCTGAAAGGAAATCAGGATAAAGTTGTTGAAGGGTATGTTACTGGCGCTTTGAGGAATCTTTGTGGCGTTGATGATGGTTATTGGAGACTGACACTAGAGGGTAGTGGTGTAGACATAGTTGTGTCTCTTTTGTCATCTGATAACCCTAATTCTCAAGCAAATGCTGCTTCTCTGCTGGCTCGTCTAGTTCTGTCCTTTTGTGATAGCATCCAGAAAATATTAAATTCTGGAGTTGTCAAGTCTCTGATACAGCTTTTGGAACAGAAAAATGATATCAATGTTCGTGCTAGTGCAGCAGATGCTTTGGAAGCTCTTTCAGCAAATTCCGATGAGGCTAAGAAATGTGTCAAAGATGCAGGAGGTGTTCATGCTCTCATTGAAGCCATTGTTGCTCCATCGAAAGAGTGTATGCAGGGAAAACATGGACAATCTCTACAAGAACATGCAACTGGAGCTTTAGCAAATGTGTTTGGTGGGATGCGCCATTTGATTATATATCTTGGACAAGTCTCACAATCCCCTCGTCTAACGGAACCAATTGGTGATGTAATTGGAGCCCTTGCCTATGCTCTAATGATTTTCAAACAACCAGAGAGTTCCGAGAATATATTTGATCCAAGCGTGATAGAGAGCATTTTGGTAAAGTTACTAAAGCCTCGTGACACAAAGTTAATCCAAGAACGGATCTTAGAGGCTATGGCGAGCTTGTACGGAAACAGTAGTCTGTCGTGTTACCTAGATGATGCAGAAGCTAAGAGAGTTCTTATCGCTCTCATAACCATGGCTTCTGCTGATGTACGAGAGCGTCTGATAATTTGCTTGTCTGGCTTATGCCATGACAAGGTTGGAATTTGGGAGGCGATTGGAAAGAGAGAAGGAATCCAGTTATTTATATCATTTCTAGGTTTGTCTAGTGAGCAACACCAAGAGTATGCTGTTGAAATGTTGAAAATTTTGACGGCTCAGGTGGATGATAGTAAATGGGCTGTAACAGCAGCCGGGGGGATACCTCCACTTGTCCAGTTGCTCGAGACTGGATCTCAGAAGGCCAAGGAAGATGCTGCCTGCATTCTCTGGAATTTGTGTTGTCACAGTGAGGAAATCCGTGATTGTGTTGAAAGAGCTGGTGGTATTCCAGCATTCTTGTGGCTTCTGAAGACTGGTGGACCAAATTCCCAGGAAACATCTGCAAAGACACTTGTGAAGCTTGTCCATACAGCTGATCCTGCCACAATAAATCAGTTGTTGGCTTTACTTCTGGGAGATGATCCGACTTCAAAGATTCAGGTAATAGAAGTGTTGGGTCATGTGCTCTCAAAAGCTTCACAAGAGGATCTTGTTCATAGAGGATGTGCCGCTAACAAAGGACTGAGGTCCCTAGTCGAGTCCCTAACATCTTCCAGAGAAGAAACAAAGGAGCATACAGCTTCAGTTTTAGCTGATCTGTTCAGCTCAAGACAAGATATTTGTGGTCATCTTGCGACGGATGACATTATTAATCCCTGGATCAAGCTGCTTACAAACAACACTCAGAATGTGGCAAAACAGGTGGCTAGGGCTTTGGACGCACTGTCCCGCCCAGTTAAGAACAATAATAATAAAAAGAAGTCTTACATTGCCGAAGGAGATATTAAGTCACTCATAAAATTGGCTAAGAACTCTTCCATTGAGTCTGCAGAAAATGCGGTATCTGCCCTCGCAAATCTTCTGTCTGATCCGGATATAGCTGCAGAAGCACTAGCTGAAGATGTTGTTTCCGCTTTCACAAGAATTTTGGCTGATGGATCTCCAGAGGGTAAGAGAAATGCGTCACGCGCACTTCATCAGTTACTTAAGAACTTTCCAGTTTGTGATGTGCTAAAGGGAAGTGCTCAGTGCCGTTTTGCTATACTTTCACTCGTTGATTCTCTAAAATCAATTGATGTGGATAGCGCAGATGCTTTCAACATATTAGAAGTAGTTGCGCTCTTGGCAAAGACTAAGAGTGGGGTGAATTTCTCTTACCCTCCATGGATTGCCCTTGCCGAAGTGCCATCAAGCCTGGAGACTCTTGTGCAATGCCTTGCCGAAGGCCATACTCTTGTGCAAGATAAGGCAATAGAAGTTTTGTCCAGGCTTTGCTCTGATCAACAGTTTCTGCTCAGTGAACTTATAGTTTCAAGACCCAAGTCAATGTTGGTTCTGGCTGATAGAATAGTGAATGCATCCAGCTTAGAAGTGAGAGTTGGGAGCACAGCGCTGCTCTTATGTGCTGCAAAGGAAAAGAAGCAGCTGATCACGGAGACACTTGATCAGTCTGGTTTTTTAAAGCTCTTGTTACATGCCCTCGTTGATATGATAAAGCACAATTCTACAAGCTTTTCTCTAGAGACTGAAGTCCAGACACCTAAAGGTTTTTTGGAAAAAAATGTTTTCCAGGATACAGGTAGTTTTTATTTTCCTGATCCAGCTAAAATCTTGGGTGGTACAGTCGCATTGTGGCTGCTATGCATACTCACTTCCGTTGATGCTAAGTCAAAGGTCATTGTTATGGAAGCTGGCGGACTTGAGGTGCTCGTGGGGAAGCTTGCTAGGTATACTTCCAGTGCTCAGGCTGAATTTGAGGATACAGAAGGCATTTGGATTAGTGCTCTCCTCCTGGCTATCATGTTCCAGGATGACAATGTGTCCTTTTCTTCAACGACAATGCGGATCATTCCAACACTTGCAGTTTTGCTAGGATCTGATGAACTGATAGATCGGTATTTCGCTGCCCACGCAATGGCTAGTCTTGTTTGTACTAGGAACAGAGGAATAAATCTGACAATTGCAAACTCTGGTGCTGTTTCTGGGATAATAAACCTTCTTGGCTATGTGGAATCGGAGATACTGAACTTGGTTGCTTTGGCAAATGAATTTTCTCTCGTGAAAGAGCCTGATCAAGTTATTCTTCAACACCTTTTTGAAATTGAAGATGTGAGGCTTGGCTCCACTGCACGTAAATCTATCCCGCTGCTTGTAGATCTCTTAAGGCCAATTCCAGATAGGCCTGGAGCCCCTCAGTTTGCTGTCCAAATCCTGATTCGCATTGCTGATGGAAGTGACACAAATAAATTACTTATGGCTGAAGCTGGAGCTGTTGAAGCTTTAACAAAGTACCTTTCTCTAAGTCCTCAAGATTCAACTGAGTACGCCATATCTGAATTGCTTAGGGTATTATTTAGCAACCACGAGCTTAGACAAAACGAAATGGCACTAAGTTCCTTGAATCAGCTTATCGCAGTACTGCGGCTAGGTTCACGAAGTGCAAGATACAGCGCAGCTGGGGCTCTGAATGAACTATTTGATGCTGAAAACATCAGAAATTCTGAAATCGCCTGCCAGGCTGTTCAACCACTTATGGACATACTCGGTTCTGTATCAGAAAGTGAGCAAGAGGTAGCTCTCTCTGCATTGATCAAACTAAGCTCAGGAAATACTTCGAATACAGCTCTTCTGATTGATGTGGAAGGAAGTCTGCTGGAAAACGTGATCAAAATCTTATCATCTGCTACTGCTTCAGAGGAGTTAAAGATAAATGCTGCCAGACTTTGTTCTGTTGTTTTCTCGAATAAAAACATCAGAACAAGTGCATCCGCCTCCGGATGCATGAAGCCGTTAATAACACTTATGCAGTCTGAAAGAAGTGCAGCAGTTGAAGCAGCAGTTTTTGCTATTAAGATATTATTGGATGACGAGCAACATTTAGAACTAGCAGCCGCACATAACATTCAGGAGCTTCTTGTTGGCTTGGTTTCCGGGAAAAATTACGTTATTATTGAGGCTAGCTTATCAGCTCTCATTAAGTTGGGAAAAGACAGAGTACCACGCAAGTTGGACATGGTGGAAGCTGGAATAATTGAGCGATGTCTTGAGCTACTACCTGGAGCCTCGAGTTCGTTATGTTCCGCTGTTGTAGAGCTGTTTCGTATTTTAACAAATAGTGGAGTAATCGCGAGAAGACCAGATGTTGCAAAAACTGTAGAGCCTCTCTTCGCGGTCTTGCTGCGGTCAGACTTGACCTTATGGGGGCAACATAGTGCCCTGCAAGCACTCGTAAACATATTGGAAAAACAGCAAACCCTTGAAGCTTTTAGTTTTACACCCAGTGAAGCCATTGTGCCGCTGATTTCTTTTTTAGAATCTTCATCCCAAGCTATCCAGCAGCTTGGAGCAGAACTGCTGAGCCATTTTCTTACAATGGAAGATTTCCAGCAAGACATCACAACACAGAGTGCTGTTGTTCCTCTGGTCCGGCTTGCAGGAATTGGAATACTGAGCCTGCAGGAAACTGCGATAAAGGCATTGGAGAAGATATCTGCTAGCTGGCCTAAGGCTGTTCTTGATGCTGAAGGTATTTTTGAGCTATCCAAGGTTATTCTTCAGGAAGATCCTCAGCCCCCTCTTGATCTATGGGAGTCAGCTGCCTTTGTTCTTTCCAACATTCTGCAGTATGATGCAGAATGCTTTTTCAGAGTTGAACTGCCAGTGTTGGTAAAATTGTTGTTCTCGACAATTGAAAGCACAGTGTTACTGGCTCTTAAAGCTTTGATGCTTCATGAGAAGAACGACGCTTCAAGTACAGTACAAATGGCTGAGCTTGGTGCAATCGATGCATTGCTGGACCTCCTGAGATCTCATCAATGCGAGGAAGAATCTGGAAGCTTACTTGAAGTAATATTTAACAATCCAAGAGTAAGAGAGTTGAAGTTATGCAAATATGCAATAGCACCACTATCTCAATATCTGTTGGACCCTCATACAAGATCAGAACCTGGTAGGCTTCTTGCTGCTCTGGCACTGGGGGATCTATCTCAGCATGAAGGGCTTTCTAGATCCAGTGGTTCTGTTTCCGCTTGTCGGGCACTCATTAGCGTGCTAGAAGAACAACCAACAGAAGAAATGAAAGTGGTGGCCATCTGTGCGTTGCAGAATTTTGTGATGAATAGTAGAACGAATAGACGAGCTGTTGCAGAGGCTGGTGGCGTATTGTTAATTCAAGAGCTACTGCTTTCTTGCAACCCAGAAGTTTCTGGGCAGGCTGCACTGATGGTCAAATTTTTGTTTTCCAACCACACACTCCAAGAATATGTCTCGAATGAGCTTATAAGATCACTAACAGCTGCACTTGAGAGAGGCTTGTGGTCTACAGCAACTATAAATATTGAAGTTTTGAGAACCTTAAATGTGATTTTCTCCAATTTTCCTAAGCTCCGTGCCTCAGAAGCTGCTACTTTCTGCATCCCTCATTTGGTAGGGGCGCTCAAATCTGGTGTTGAAGACGTCCAGGGATTAGTATTGGATATTCTTTACTTGCTAAGACACTCCTGGACGAATATGTCTATAGATGTTGCAAAGTCTCAAGCCATGATTGCAGCTGAAGCAATTCCCGTCCTGCAAATGCTGATGAAAACATGCCCTCCTAGGTTTCACGACAAGGCAGATAGCTTATTGCACTGCTTACCAGGTTGCTTAACAGTGAACGTCATGCGTGCCAATAACTTAAAGCAGTCTATGGCAACCACAAACGCCTTTTGTCAATTAACAATAGGAAACTGCCCTCCACGGCAAACTAAGGTCGTGAGCAATAGCACTACTCCTGAATGGAAAGAAGGCTTTACTTGGGCATTTGATGTTCCCCCCAAAGGACAAAAGCTTCACATCATATGCAAGAGCAAAAGCACATTTGGAAAGACGACTCTAGGGCGAGTTACTATCCAAATCGACAAAGTCGTGACAGAAGGAGAGTACAGTGGATCTCTAAGCCTGAATCATGAAAATAGCAAAGATGCTTCTTCCAGATCACTCGACATTGAGATAGCATGGTCCAATAGGACAACGGATGAGACTCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000271 GO:0000902 GO:0002682 GO:0002683 GO:0003674 GO:0003824 GO:0004842 GO:0005102 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005856 GO:0005874 GO:0005881 GO:0005886 GO:0005938 GO:0005975 GO:0005976 GO:0006073 GO:0006464 GO:0006807 GO:0006950 GO:0006952 GO:0008104 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009250 GO:0009605 GO:0009607 GO:0009617 GO:0009653 GO:0009664 GO:0009826 GO:0009832 GO:0009987 GO:0010330 GO:0010383 GO:0010941 GO:0015630 GO:0016020 GO:0016043 GO:0016049 GO:0016051 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0030243 GO:0030244 GO:0030863 GO:0030981 GO:0032446 GO:0032502 GO:0032989 GO:0032991 GO:0033036 GO:0033365 GO:0033612 GO:0033692 GO:0034613 GO:0034637 GO:0034645 GO:0036211 GO:0040007 GO:0042546 GO:0042742 GO:0042981 GO:0043066 GO:0043067 GO:0043069 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043412 GO:0044036 GO:0044038 GO:0044042 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044262 GO:0044264 GO:0044267 GO:0044380 GO:0044422 GO:0044424 GO:0044430 GO:0044444 GO:0044446 GO:0044448 GO:0044464 GO:0045229 GO:0048519 GO:0048523 GO:0048580 GO:0048583 GO:0048585 GO:0048589 GO:0048856 GO:0048869 GO:0050776 GO:0050777 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051179 GO:0051239 GO:0051273 GO:0051274 GO:0051641 GO:0051704 GO:0051707 GO:0052324 GO:0052541 GO:0055028 GO:0060548 GO:0060560 GO:0065007 GO:0070589 GO:0070592 GO:0070647 GO:0070696 GO:0070727 GO:0071554 GO:0071555 GO:0071669 GO:0071704 GO:0071840 GO:0071944 GO:0072697 GO:0072698 GO:0072699 GO:0098542 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0099568 GO:0140096 GO:1901564 GO:1901576 GO:1902494 GO:1990234 GO:1990778 GO:2000026 GO:2000028 GO:2000241
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Arm C2

Protein Analysis

2136

Amino Acids

231.43

Weight (kDa)

5.41

Isoelectric Point (pI)

39.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Arm PF00514 504 - 541 1.6e-07 Armadillo/beta-catenin-like repeat
ARM_PUB PF25598 1314 - 1493 3.9e-07 Plant U-box protein ARM repeat region
C2 PF00168 2009 - 2106 1.9e-13 C2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 5663
AasI GACNNNNNNGTC 1 cut(s) 5893
AatII GACGTC 1 cut(s) 5841
AccB7I CCANNNNNTGG 1 cut(s) 3298
AccI GTMKAC 4 cut(s) 187, 648, 3113, 5702
AccII CGCG 3 cut(s) 2352, 4511, 4553
AccIII TCCGGA 3 cut(s) 401, 2254, 4157
AcoI YGGCCR 3 cut(s) 241, 291, 5469
AcuI CTGAAG 9 cut(s) 1712, 1950, 2301, 2931, 3663, 4059, 4922, 4926, 5955
AcyI GRCGYC 1 cut(s) 5838
AdeI CACNNNGTG 3 cut(s) 3821, 4664, 4790
AfeI AGCGCT 1 cut(s) 2775
AfiI CCNNNNNNNGG 7 cut(s) 100, 1378, 3298, 4584, 5415, 5806, 6200
AflII CTTAAG 2 cut(s) 2369, 3544
AhdI GACNNNNNGTC 2 cut(s) 2107, 3202
AjuI GAANNNNNNNTTGG 4 cut(s) 2745, 2777, 3582, 3614
Alw21I GWGCWC 6 cut(s) 1851, 2410, 2771, 3093, 3127, 3169
AlwNI CAGNNNCTG 4 cut(s) 2674, 5330, 5534, 5783
Ama87I CYCGRG 2 cut(s) 1565, 4447
Aor13HI TCCGGA 3 cut(s) 401, 2254, 4157
Aor51HI AGCGCT 1 cut(s) 2775
AoxI GGCC 9 cut(s) 9, 241, 291, 1584, 2608, 3548, 3563, 4883, 5469
ArsI GACNNNNNNTTYG 6 cut(s) 288, 320, 1986, 2018, 2231, 2263
AseI ATTAAT 1 cut(s) 2034
Asp700I GAANNNNTTC 2 cut(s) 3994, 5940
AspA2I CCTAGG 1 cut(s) 5978
AspLEI GCGC 8 cut(s) 584, 1020, 2354, 2471, 2503, 2776, 3836, 5818
AspS9I GGNCC 6 cut(s) 1703, 1911, 4807, 5179, 5308, 6381
AsuC2I CCSGG 2 cut(s) 1537, 4315
AsuHPI GGTGA 3 cut(s) 147, 1091, 2539
AsuII TTCGAA 1 cut(s) 3998
AsuNHI GCTAGC 2 cut(s) 4340, 4877
AvaI CYCGRG 2 cut(s) 1565, 4447
AvaII GGWCC 6 cut(s) 1703, 1911, 4807, 5179, 5308, 6381
AvrII CCTAGG 1 cut(s) 5978
AxyI CCTNAGG 1 cut(s) 4887
BaeGI GKGCMC 2 cut(s) 4600, 5422
BalI TGGCCA 3 cut(s) 243, 293, 5471
BanII GRGCYC 3 cut(s) 1096, 3574, 3847
BauI CACGAG 4 cut(s) 1194, 3092, 3440, 3746
BbsI GAAGAC 3 cut(s) 1700, 4522, 5841
Bbv12I GWGCWC 6 cut(s) 1851, 2410, 2771, 3093, 3127, 3169
BbvCI CCTCAGC 1 cut(s) 4950
BccI CCATC 7 cut(s) 605, 940, 2312, 2578, 3605, 5480, 5599
BceAI ACGGC 4 cut(s) 1512, 2400, 4156, 6137
BciVI GTATCC 3 cut(s) 1536, 2950, 3136
BclI TGATCA 6 cut(s) 2662, 2811, 2827, 3454, 3972, 4047
BcnI CCSGG 2 cut(s) 1537, 4315
BfmI CTRYAG 8 cut(s) 111, 2211, 2265, 4536, 4838, 5003, 5703, 5898
BfoI RGCGCY 3 cut(s) 585, 2777, 5819
BfrI CTTAAG 2 cut(s) 2369, 3544
BfuI GTATCC 3 cut(s) 1536, 2950, 3136
BglI GCCNNNNNGGC 1 cut(s) 3899
BglII AGATCT 2 cut(s) 3538, 5189
BlnI CCTAGG 1 cut(s) 5978
BlpI GCTNAGC 3 cut(s) 120, 4739, 5151
BmcAI AGTACT 1 cut(s) 3801
Bme18I GGWCC 6 cut(s) 1703, 1911, 4807, 5179, 5308, 6381
BmeRI GACNNNNNGTC 2 cut(s) 2107, 3202
BmeT110I CYCGRG 2 cut(s) 1565, 4447
BmgT120I GGNCC 6 cut(s) 1703, 1911, 4807, 5179, 5308, 6381
BmiI GGNNCC 8 cut(s) 406, 1071, 1093, 1913, 3505, 3571, 4444, 5310
BmrI ACTGGG 3 cut(s) 2111, 4656, 5366
BmtI GCTAGC 2 cut(s) 4344, 4881
BmuI ACTGGG 3 cut(s) 2111, 4656, 5366
BoxI GACNNNNGTC 3 cut(s) 1909, 2704, 2912
BpiI GAAGAC 3 cut(s) 1700, 4522, 5841
BpmI CTGGAG 7 cut(s) 786, 1008, 2310, 2600, 3588, 3669, 4461
Bpu10I CCTNAGC 6 cut(s) 1500, 3126, 3725, 3985, 4950, 5763
Bpu1102I GCTNAGC 3 cut(s) 120, 4739, 5151
Bpu14I TTCGAA 1 cut(s) 3998
BpuEI CTTGAG 7 cut(s) 675, 1975, 3104, 3675, 4448, 5705, 5901
BpuMI CCSGG 2 cut(s) 1537, 4315
Bsa29I ATCGAT 1 cut(s) 5166
BsaAI YACGTR 1 cut(s) 3515
BsaBI GATNNNNATC 1 cut(s) 4052
BsaHI GRCGYC 1 cut(s) 5838
BsaWI WCCGGW 3 cut(s) 401, 2254, 4157
BsaXI ACNNNNNCTCC 2 cut(s) 5959, 5989
Bsc4I CCNNNNNNNGG 7 cut(s) 100, 1378, 3298, 4584, 5415, 5806, 6200
Bse21I CCTNAGG 1 cut(s) 4887
Bse3DI GCAATG 7 cut(s) 111, 255, 2151, 2600, 3303, 3603, 5171
Bse8I GATNNNNATC 1 cut(s) 4052
BseAI TCCGGA 3 cut(s) 401, 2254, 4157
BseCI ATCGAT 1 cut(s) 5166
BseJI GATNNNNATC 1 cut(s) 4052
BseLI CCNNNNNNNGG 7 cut(s) 100, 1378, 3298, 4584, 5415, 5806, 6200
BseMI GCAATG 7 cut(s) 111, 255, 2151, 2600, 3303, 3603, 5171
BseRI GAGGAG 3 cut(s) 47, 3161, 4094
BseSI GKGCMC 2 cut(s) 4600, 5422
BseYI CCCAGC 1 cut(s) 3839
BsgI GTGCAG 5 cut(s) 849, 3495, 4224, 5583, 5664
Bsh1236I CGCG 3 cut(s) 2352, 4511, 4553
BshFI GGCC 9 cut(s) 11, 243, 293, 1586, 2610, 3550, 3565, 4885, 5471
BshVI ATCGAT 1 cut(s) 5166
BsiHKAI GWGCWC 6 cut(s) 1851, 2410, 2771, 3093, 3127, 3169
BsiHKCI CYCGRG 2 cut(s) 1565, 4447
BsiSI CCGG 6 cut(s) 402, 1536, 2255, 4158, 4314, 4810
BslFI GGGAC 4 cut(s) 295, 1897, 1909, 2095
BslI CCNNNNNNNGG 7 cut(s) 100, 1378, 3298, 4584, 5415, 5806, 6200
BsmFI GGGAC 4 cut(s) 295, 1897, 1909, 2095
BsmI GAATGC 4 cut(s) 1605, 1676, 2743, 5024
BsnI GGCC 9 cut(s) 11, 243, 293, 1586, 2610, 3550, 3565, 4885, 5471
BsoBI CYCGRG 2 cut(s) 1565, 4447
Bsp119I TTCGAA 1 cut(s) 3998
Bsp13I TCCGGA 3 cut(s) 401, 2254, 4157
Bsp1720I GCTNAGC 3 cut(s) 120, 4739, 5151
Bsp19I CCATGG 3 cut(s) 408, 1317, 2546
Bsp68I TCGCGA 1 cut(s) 4511
BspANI GGCC 9 cut(s) 11, 243, 293, 1586, 2610, 3550, 3565, 4885, 5471
BspDI ATCGAT 1 cut(s) 5166
BspEI TCCGGA 3 cut(s) 401, 2254, 4157
BspFNI CGCG 3 cut(s) 2352, 4511, 4553
BspHI TCATGA 2 cut(s) 5113, 6328
BspLI GGNNCC 8 cut(s) 406, 1071, 1093, 1913, 3505, 3571, 4444, 5310
BspMAI CTGCAG 4 cut(s) 2215, 2269, 4842, 5007
BspOI GCTAGC 2 cut(s) 4344, 4881
BspQI GCTCTTC 1 cut(s) 4014
BspT104I TTCGAA 1 cut(s) 3998
BspTI CTTAAG 2 cut(s) 2369, 3544
BsrDI GCAATG 7 cut(s) 111, 255, 2151, 2600, 3303, 3603, 5171
BssNAI GTATAC 1 cut(s) 3114
BssNI GRCGYC 1 cut(s) 5838
BssSI CACGAG 4 cut(s) 1194, 3092, 3440, 3746
BssT1I CCWWGG 6 cut(s) 408, 1317, 1587, 2546, 4926, 5978
Bst1107I GTATAC 1 cut(s) 3114
Bst2BI CACGAG 4 cut(s) 1194, 3092, 3440, 3746
Bst6I CTCTTC 3 cut(s) 2203, 4014, 4553
BstACI GRCGYC 1 cut(s) 5838
BstAFI CTTAAG 2 cut(s) 2369, 3544
BstAPI GCANNNNNTGC 5 cut(s) 701, 836, 2219, 4226, 5168
BstBAI YACGTR 1 cut(s) 3515
BstBI TTCGAA 1 cut(s) 3998
BstDSI CCRYGG 4 cut(s) 408, 1317, 2546, 6119
BstFNI CGCG 3 cut(s) 2352, 4511, 4553
BstH2I RGCGCY 3 cut(s) 585, 2777, 5819
BstHHI GCGC 8 cut(s) 584, 1020, 2354, 2471, 2503, 2776, 3836, 5818
BstNSI RCATGY 3 cut(s) 983, 2861, 5973
BstPAI GACNNNNGTC 3 cut(s) 1909, 2704, 2912
BstSFI CTRYAG 8 cut(s) 111, 2211, 2265, 4536, 4838, 5003, 5703, 5898
BstSLI GKGCMC 2 cut(s) 4600, 5422
BstUI CGCG 3 cut(s) 2352, 4511, 4553
BstV2I GAAGAC 3 cut(s) 1700, 4522, 5841
BstXI CCANNNNNNTGG 2 cut(s) 522, 4724
BstZ17I GTATAC 1 cut(s) 3114
Bsu15I ATCGAT 1 cut(s) 5166
Bsu36I CCTNAGG 1 cut(s) 4887
BsuI GTATCC 3 cut(s) 1536, 2950, 3136
BsuRI GGCC 9 cut(s) 11, 243, 293, 1586, 2610, 3550, 3565, 4885, 5471
BsuTUI ATCGAT 1 cut(s) 5166
BtgI CCRYGG 4 cut(s) 408, 1317, 2546, 6119
BtgZI GCGATG 1 cut(s) 4435
BtsI GCAGTG 2 cut(s) 3507, 6010
BtuMI TCGCGA 1 cut(s) 4511
CaiI CAGNNNCTG 4 cut(s) 2674, 5330, 5534, 5783
CciI TCATGA 2 cut(s) 5113, 6328
CfoI GCGC 8 cut(s) 584, 1020, 2354, 2471, 2503, 2776, 3836, 5818
Cfr13I GGNCC 6 cut(s) 1703, 1911, 4807, 5179, 5308, 6381
ClaI ATCGAT 1 cut(s) 5166
CseI GACGC 4 cut(s) 1331, 2111, 2334, 5135
CsiI ACCWGGT 2 cut(s) 5329, 6019
CspCI CAANNNNNGTGG 6 cut(s) 2067, 2102, 2442, 2477, 4371, 4406
DraI TTTAAA 1 cut(s) 2844
DraIII CACNNNGTG 3 cut(s) 3821, 4664, 4790
DrdI GACNNNNNNGTC 1 cut(s) 5893
DriI GACNNNNNGTC 2 cut(s) 2107, 3202
DseDI GACNNNNNNGTC 1 cut(s) 5893
EaeI YGGCCR 3 cut(s) 241, 291, 5469
Eam1104I CTCTTC 3 cut(s) 2203, 4014, 4553
Eam1105I GACNNNNNGTC 2 cut(s) 2107, 3202
EarI CTCTTC 3 cut(s) 2203, 4014, 4553
EciI GGCGGA 2 cut(s) 3093, 4141
Eco130I CCWWGG 6 cut(s) 408, 1317, 1587, 2546, 4926, 5978
Eco147I AGGCCT 2 cut(s) 11, 3565
Eco24I GRGCYC 3 cut(s) 1096, 3574, 3847
Eco32I GATATC 2 cut(s) 811, 4872
Eco47I GGWCC 6 cut(s) 1703, 1911, 4807, 5179, 5308, 6381
Eco47III AGCGCT 1 cut(s) 2775
Eco57I CTGAAG 9 cut(s) 1712, 1950, 2301, 2931, 3663, 4059, 4922, 4926, 5955
Eco81I CCTNAGG 1 cut(s) 4887
Eco88I CYCGRG 2 cut(s) 1565, 4447
EcoO109I RGGNCCY 1 cut(s) 1911
EcoRV GATATC 2 cut(s) 811, 4872
EcoT14I CCWWGG 6 cut(s) 408, 1317, 1587, 2546, 4926, 5978
EcoT22I ATGCAT 5 cut(s) 374, 2743, 3029, 4166, 5173
EcoT38I GRGCYC 3 cut(s) 1096, 3574, 3847
ErhI CCWWGG 6 cut(s) 408, 1317, 1587, 2546, 4926, 5978
FalI AAGNNNNNCTT 4 cut(s) 1857, 1889, 3450, 3482
FaqI GGGAC 4 cut(s) 295, 1897, 1909, 2095
FauI CCCGC 3 cut(s) 384, 2120, 3534
FauNDI CATATG 1 cut(s) 6221
FbaI TGATCA 6 cut(s) 2662, 2811, 2827, 3454, 3972, 4047
FblI GTMKAC 4 cut(s) 187, 648, 3113, 5702
FriOI GRGCYC 3 cut(s) 1096, 3574, 3847
GlaI GCGC 8 cut(s) 583, 1019, 2353, 2470, 2502, 2775, 3835, 5817
GsaI CCCAGC 1 cut(s) 3843
GsuI CTGGAG 7 cut(s) 786, 1008, 2310, 2600, 3588, 3669, 4461
HaeII RGCGCY 3 cut(s) 585, 2777, 5819
HaeIII GGCC 9 cut(s) 11, 243, 293, 1586, 2610, 3550, 3565, 4885, 5471
HapII CCGG 6 cut(s) 402, 1536, 2255, 4158, 4314, 4810
HgaI GACGC 4 cut(s) 1331, 2111, 2334, 5135
HhaI GCGC 8 cut(s) 584, 1020, 2354, 2471, 2503, 2776, 3836, 5818
Hin1I GRCGYC 1 cut(s) 5838
Hin6I GCGC 8 cut(s) 582, 1018, 2352, 2469, 2501, 2774, 3834, 5816
HinP1I GCGC 8 cut(s) 582, 1018, 2352, 2469, 2501, 2774, 3834, 5816
HindIII AAGCTT 9 cut(s) 1742, 1857, 2893, 3101, 3660, 4644, 5100, 5218, 6209
HpaII CCGG 6 cut(s) 402, 1536, 2255, 4158, 4314, 4810
HphI GGTGA 3 cut(s) 147, 1091, 2539
Hpy166II GTNNAC 9 cut(s) 188, 649, 1703, 2684, 3114, 3815, 4615, 5703, 6037
Hpy8I GTNNAC 9 cut(s) 188, 649, 1703, 2684, 3114, 3815, 4615, 5703, 6037
Hpy99I CGWCG 2 cut(s) 2023, 5129
HpyCH4IV ACGT 5 cut(s) 3514, 4044, 4326, 5838, 6039
HpySE526I ACGT 5 cut(s) 3514, 4044, 4326, 5838, 6039
Hsp92I GRCGYC 1 cut(s) 5838
HspAI GCGC 8 cut(s) 582, 1018, 2352, 2469, 2501, 2774, 3834, 5816
Kpn2I TCCGGA 3 cut(s) 401, 2254, 4157
Ksp22I TGATCA 6 cut(s) 2662, 2811, 2827, 3454, 3972, 4047
LguI GCTCTTC 1 cut(s) 4014
MabI ACCWGGT 2 cut(s) 5329, 6019
MaeII ACGT 5 cut(s) 3514, 4044, 4326, 5838, 6039
MfeI CAATTG 4 cut(s) 1074, 2454, 3342, 5070
MlsI TGGCCA 3 cut(s) 243, 293, 5471
MluNI TGGCCA 3 cut(s) 243, 293, 5471
MlyI GAGTC 6 cut(s) 1931, 2216, 2578, 4982, 6247, 6397
MmeI TCCRAC 8 cut(s) 539, 1363, 1832, 3260, 4371, 5019, 5286, 5649
Mox20I TGGCCA 3 cut(s) 243, 293, 5471
Mph1103I ATGCAT 5 cut(s) 374, 2743, 3029, 4166, 5173
MroI TCCGGA 3 cut(s) 401, 2254, 4157
MroXI GAANNNNTTC 2 cut(s) 3994, 5940
MscI TGGCCA 3 cut(s) 243, 293, 5471
MslI CAYNNNNRTG 6 cut(s) 482, 656, 1316, 1681, 3065, 5429
Msp20I TGGCCA 3 cut(s) 243, 293, 5471
MspA1I CMGCKG 9 cut(s) 1757, 2809, 3529, 3839, 4466, 4681, 4979, 5678, 5933
MspCI CTTAAG 2 cut(s) 2369, 3544
MspI CCGG 6 cut(s) 402, 1536, 2255, 4158, 4314, 4810
MunI CAATTG 4 cut(s) 1074, 2454, 3342, 5070
Mva1269I GAATGC 4 cut(s) 1605, 1676, 2743, 5024
MvnI CGCG 3 cut(s) 2352, 4511, 4553
NciI CCSGG 2 cut(s) 1537, 4315
NcoI CCATGG 3 cut(s) 408, 1317, 2546
NdeI CATATG 1 cut(s) 6221
NheI GCTAGC 2 cut(s) 4340, 4877
NlaIV GGNNCC 8 cut(s) 406, 1071, 1093, 1913, 3505, 3571, 4444, 5310
NmuCI GTSAC 6 cut(s) 1196, 1625, 2172, 2346, 3617, 6289
NruI TCGCGA 1 cut(s) 4511
NsiI ATGCAT 5 cut(s) 374, 2743, 3029, 4166, 5173
NspI RCATGY 3 cut(s) 983, 2861, 5973
NspV TTCGAA 1 cut(s) 3998
PaeR7I CTCGAG 2 cut(s) 1565, 4447
PagI TCATGA 2 cut(s) 5113, 6328
PceI AGGCCT 2 cut(s) 11, 3565
PciSI GCTCTTC 1 cut(s) 4014
PcsI WCGNNNNNNNCGW 1 cut(s) 6285
PctI GAATGC 4 cut(s) 1605, 1676, 2743, 5024
PdmI GAANNNNTTC 2 cut(s) 3994, 5940
PflFI GACNNNGTC 1 cut(s) 6284
PflMI CCANNNNNTGG 1 cut(s) 3298
PfoI TCCNGGA 5 cut(s) 19, 2952, 3189, 4313, 5882
PleI GAGTC 6 cut(s) 1930, 2215, 2578, 4981, 6247, 6397
PpsI GAGTC 6 cut(s) 1930, 2215, 2578, 4981, 6247, 6397
Ppu21I YACGTR 1 cut(s) 3515
PpuMI RGGWCCY 1 cut(s) 1911
PshAI GACNNNNGTC 3 cut(s) 1909, 2704, 2912
PshBI ATTAAT 1 cut(s) 2034
PsiI TTATAA 1 cut(s) 5663
Psp5II RGGWCCY 1 cut(s) 1911
PspFI CCCAGC 1 cut(s) 3839
PspN4I GGNNCC 8 cut(s) 406, 1071, 1093, 1913, 3505, 3571, 4444, 5310
PspPI GGNCC 6 cut(s) 1703, 1911, 4807, 5179, 5308, 6381
PspPPI RGGWCCY 1 cut(s) 1911
PspXI VCTCGAGB 1 cut(s) 4447
PstI CTGCAG 4 cut(s) 2215, 2269, 4842, 5007
PstNI CAGNNNCTG 4 cut(s) 2674, 5330, 5534, 5783
PsyI GACNNNGTC 1 cut(s) 6284
PvuII CAGCTG 6 cut(s) 1757, 2809, 3839, 4979, 5678, 5933
RruI TCGCGA 1 cut(s) 4511
RseI CAYNNNNRTG 6 cut(s) 482, 656, 1316, 1681, 3065, 5429
SapI GCTCTTC 1 cut(s) 4014
Sau96I GGNCC 6 cut(s) 1703, 1911, 4807, 5179, 5308, 6381
SbfI CCTGCAGG 1 cut(s) 4842
ScaI AGTACT 1 cut(s) 3801
SchI GAGTC 6 cut(s) 1931, 2216, 2578, 4982, 6247, 6397
SdaI CCTGCAGG 1 cut(s) 4842
SexAI ACCWGGT 2 cut(s) 5329, 6019
SfcI CTRYAG 8 cut(s) 111, 2211, 2265, 4536, 4838, 5003, 5703, 5898
Sfr274I CTCGAG 2 cut(s) 1565, 4447
SfuI TTCGAA 1 cut(s) 3998
SinI GGWCC 6 cut(s) 1703, 1911, 4807, 5179, 5308, 6381
SlaI CTCGAG 2 cut(s) 1565, 4447
SmiMI CAYNNNNRTG 6 cut(s) 482, 656, 1316, 1681, 3065, 5429
Sse8387I CCTGCAGG 1 cut(s) 4842
SseBI AGGCCT 2 cut(s) 11, 3565
SspI AATATT 4 cut(s) 492, 756, 5235, 5719
StuI AGGCCT 2 cut(s) 11, 3565
StyI CCWWGG 6 cut(s) 408, 1317, 1587, 2546, 4926, 5978
TaiI ACGT 5 cut(s) 3517, 4047, 4329, 5841, 6042
TatI WGTACW 5 cut(s) 3314, 3799, 5135, 5140, 6303
TauI GCSGC 4 cut(s) 1895, 3808, 4277, 4681
TseFI GTSAC 6 cut(s) 1196, 1625, 2172, 2346, 3617, 6289
Tsp45I GTSAC 6 cut(s) 1196, 1625, 2172, 2346, 3617, 6289
TspGWI ACGGA 9 cut(s) 1082, 1234, 1263, 1631, 2036, 2831, 3028, 5759, 6410
Tth111I GACNNNGTC 1 cut(s) 6284
Van91I CCANNNNNTGG 1 cut(s) 3298
Vha464I CTTAAG 2 cut(s) 2369, 3544
VpaK11BI GGWCC 6 cut(s) 1703, 1911, 4807, 5179, 5308, 6381
VspI ATTAAT 1 cut(s) 2034
XbaI TCTAGA 2 cut(s) 2902, 5387
XceI RCATGY 3 cut(s) 983, 2861, 5973
XcmI CCANNNNNNNNNTGG 3 cut(s) 1379, 1680, 2710
XhoI CTCGAG 2 cut(s) 1565, 4447
XmaJI CCTAGG 1 cut(s) 5978
XmiI GTMKAC 4 cut(s) 187, 648, 3113, 5702
XmnI GAANNNNTTC 2 cut(s) 3994, 5940
ZraI GACGTC 1 cut(s) 5839
ZrmI AGTACT 1 cut(s) 3801
Zsp2I ATGCAT 5 cut(s) 374, 2743, 3029, 4166, 5173
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.