MD00G1028700.v1.1

Protein kinase C conserved region 2 (CalB)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0009147
Physical Location & Seq
Forward (+)
4724315 .. 4732315
8001 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1028700.v1.1.491

Sequence Viewer

Length: 6144 bp
ATGTCAAAAGCTCCCTCCTCTAAACCGCGAGAACCCGTATCGTCTTCTACTTCACAGTCTAGGGATTTAAATGAACCAGCAATGGATGATGAAGAAGGTACAATGGCAAGAGTTGCTCAATTTATTGAGCAACTACATGCCAGCATGTCTTCACTGCAAGAAAAAGAGCTTATTACAGCACGTTTGCTAGGTATTGCCAAAGCAAGAAAGGATGCAAGAACAATTATTGGTTCGCATTCCCAGGCAATGCCATTGTTCATAAGCATTCTCAGGAACGGCACCCCTGTGGCAAAAGTTAAGGTGGCTGCAACTCTGAGTGTCCTGTGTAAAGATGAAGACTTGCGTCTTAAAGTACTTCTAGGCGGGTGCATCCCCCCTTTACTCTCACTTTTGAAGTCCGAATCAATTGAGGCTAGGAAGGCTGCAGCAGAAGCTATATACGAAGTTTCCTCAGGTGGACTTTCAGATGATCATGTCGGCATCAAAATATTTATCACAGAAGGTGTAGTACCAAACTTGTGGAATCAACTCAATCCAAAGAGCAAGCAGGACAAAGTGGTTGAGGGATTTGTGACAGGGGCTTTGAGAAATCTTTGTGGTGACAAGGATGGTTACTGGAAAGCAACACTCGAGGCTGGTGGAGTTGATATCATTGTGGGTCTTCTGTCTTCTGACAATGCTGCTGCCCAGTCCAATGCAGCCTCCCTCTTGGCCCGTCTCATGTTGGCTTTCAGCGATAGCATCCCTAAAGTAATAGATTCTGGAGCTGTCAAAGCTTTGCTTCAGCTTGTGGGCCAGGAAAATGATGTTTCTGTCCGTGCTAGTGCTGCTGATGCTCTGGAAGCCCTTTCTTCAAAGTCAACTGGGGCTAAAAAAGCTATTGTGAATGCAGATGGTCTTTCAGTTCTAATAGGGGCTATAGTTGCTCCATCTAAAGAGTGCATGCAAGGTGAGTGTGGGCAGGCTCTTCAAGGCCATGCCACACGGGCTTTAGCAAATATATGTGGTGGGATGTCTGCTTTAATTTTATATCTTGGAGAACTGTCACAGTCACCTCGCCTAGCTGCCCCGGTTGCTGATATAATTGGAGCTCTTGCATACACGCTGATGGTCTTTGAGCACAATTCTGGTGCAGATCAGGAATCTGTTAATGTAACAAAGATAGAGGATATTCTTGTAATGCTGCTAAAGCCTCAGGACAATAAGCTTGTTCAAGAGCGTGTCCTTGAGGCCATGGCTAGTCTATATGGCAACAACTCTCTCTCCAGTTGGCTCAATCATGCACAAGCAAAGAAGGTACTCATTGGACTTATAACAATGGCTGCCGTTGACGTGCAAGAGTATCTTATACCCTCTTTGACAAGCTTATGCTGTGATGGTACTGGAATATGGGAGTCCATTGGCAAGAGAGAAGGAATTCAGTTACTTATTTCATTGTTGGGGTTATCCAGTGAGCAGCATCAAGAATATGCTGTTCAGTTGCTGGCCATCTTAGCTGACCAAGTTGATGACAGCAAGTGGGCTATTACAGCTGCCGGTGGGATTCCTCCTCTGGTACAGTTATTAGAGACGGGTTCTCAGAAGGCAAAAGAGGATGCCGCTCATGTGTTGTGGAATTTGTGCTGTCACAGTGAAGATATTCGTGCATGTGTTGAAAGTGCAGGAGCCATCCCAGCATTTTTATGGCTTTTAAAAAGTGGTGGGTCAAGAGGACAAGAAGCATCAGCTAAGGCACTCACAAAGCTTGTCCCGAGAGCGGATTCTGCCACCATTAATCAATTGTTAGCTTTGCTCCTCGGTGACTCTCCAAGCTCGAAGGCCCATACTATCAGGGTATTGGGTCATGTTCTCATAATGGCGTCACACAATGATCTTGTGCATAAAGGTTCAGCAGCTAATAAAGGGCTGAGGTCTCTTGTCCAGGTCCTCAATTCATCAAATGAAGAAACTCAAGAGTATGCTGCGTCTGTCCTAGCTGATCTATTCAGCACAAGACAAGATATTTGTGATACTCTTGCAACTGATGAGATCGTGCATCCTTGCATGAAGCTATTGACCAGCAACACACAAGGTGTTGCAACACAGTCAGCTCGAGCATTGGGTGCTCTGTCCCGCCCTATGAAGACCAAAACAACAAGCAAGATGTCTTATTTTGCAGAAGGTGATGTCAAGCCCCTCATCAGACTGGCTAAAACTTCTTCCATTGATGCTGCTGAAACTGCAGTTGCTGCACTTGCCAATCTTCTCTCTGATCCCCAGATTGCTGCAGAAGCCCTGGCAGAAGATGTTGTTTCAGCTTTGATAAGAGTACTGGGTGATGGAACTTCAGAAGGTAAGAAGAATGCATCCCGTGCCCTTCGTCAATTCCTGAAGCATTTTCCTGTAGGTTATGTGCTCACAGGAAATGCTCAGTGTTGTTTTGCTATGCTTGCTATTGTTGATTCCTTAAATGCATTGGATATGGTTGGGACTGATGCTGCGGATGCTTTAGAAGTAGTAGCTCTTTTGGCTAGAACAAAACAGGGCGTGAACTTCACTTACCGTCCATGGTCTGCCCTTGCTGAAGTTCCATCAAGCTTAGAATCTCTTGTTCGCTGCCTGGCTGAAGGGCCTCCCCCACTTCAGGATAAGGCAATAGAAATTTTATCTAGGCTTTGTGATGAGCAACCAGTTGTGCTCGGTGATCTATTGATTGAAAGATCAAGATCCCTTGGTTCACTAGCTAACAGGGTAATGAACTCATCTAGTCTAGAAATCAGAGTTGGAGGAGCTGCGTTACTTATTTGTGCAGCAAAAGAGTACAAACAGAAGGCAATGGAAGTACTTGATGTATCAGGATATTTAAAACTGTTAGTGTATGCTTTAGTGGACATGATGAAGCAAAATTCTAGCTGCTCCTCTCTAGAAATTGAAGTCAGAACTCCTAGGGGTTTTATTGAAAGAACTGCATTCCACAAAGGTGACGAATTTGATGTCCCTGATCCAGCCATTGTCTTAGGGGGTACTGTTGCCTTGTGGTTGCTATGCATAATTGGTTCCTTTCATGCAAAGAGCAAACTCACAATTATGGAAGCCGGTGGTCTTGAGGTTCTATCTGACAAGCTTGCATGTTACACTTCCAATCCACAGGCAGAATATGAGGATACGGAAGGTATATGGATTAGTGCCTTACTCCTGGCTATTCTGTTTGAAGATGCAAATGTTGTTCTGTCACCTGTAACAATGTGCATCATTCCTTCACTTGCTCTTCTTTTGAGATCAGATGAGATGATTGATAGGTTCTTTGCTGCCCAGTCAATGGCCAGTCTTGTTTCTAATGGTAGTAAAGGAATAATTCTTGCCCTTGCAAATTCAGGTGCGGTTGCTGGATTAATAACCCTAATTGGTTATATAGAGTCCGACGTGCCTAACCTCGTTACTTTGTCAGAAGAGTTTTCTCTGGTACGAAATCCTGATCAAGTTGTTTTGGAGTACCTTTTTTATTTTGAAGATGTAAGAGTTGGATCAACAGCACGTAAGTCTATACCTCTCTTGGTGGATCTCTTGAGACCAATGCCAGAACGGCCTGGTGCCCCTCCATTTGCTGTTAAACTCTTGACCCGTATTGCCAATGGAAGTGATACAAATAAATTAATTATGGGTGAAGCTGGAGCTCTGGATGCTCTGACAAAGTACCTTTCTTTGAGCCCACAAGACTCCACAGAAGCTACAATAACTGAATTATTCAGAATATTATTCAGCAATCCCGATATCATTCGTTATGAAGCATCAGCTAGTTCTTTGAATCAACTCATAGCTGTTCTGCGTCTAGGGTCAAGAACTGCAAGATATAGTGCTGCAAGAGCTCTTCATGAACTATTTGACGCTGAGAACATCAGAGATTCTGATTTAGCAAGGCAGTCTATTCAACCATTGGTTGACATGCTTAACGCTGCATCAGAGAGTGAGCAAGAGGCTGCCCTTGTTGCGTTAATTAAGTTGACTTCAGGAAATTCTTCTAAAGCAGCTTTTTTTACTGATGGGCAGGGAAATCCACTGGAGAGTTTGTACAAAATACTGTCTTCTGCTTCATCCTTGGAATTGAGGAGAATTGCTGCACAACTCTGTTGTACTTTGTTTGATAATACCGAAGTCAGAGCAAGTGCAATTGCCTCAGAATGCGTAGAGCCCCTTATATCACTGATGCATTCTGATACAACTACAGCTGTAGAAGCTGGAGTTTGTGCTTTTGAAAAATTGTTGGATGATGAACACCGGGTGGAGCTTGCAATGGCCTATAATGTTGTGGATCTCCTTGTTGGATTAGTTTCTGGAACAAGCATGCTGCTCATTGAGGCCAGTGTCTGTTCTCTCATAAAGTTGGGGAAAGACCGGACCCCATGCAAATTGGATATGGTCAATGCTGGAATTATTGATAAATGTCTTGAGCTACTACCTGCTGCACCCAGTTCATTATGCTCTTCCGTGGCTGAATTGTTCCGCATTTTAACAAATAGTAATGCAATTGCTAGAAGTTTGGGTGCTGCACAAATTGTAGAACCTCTTTTTATAGTTTTACAACGTCCAGATTTCAATTTATGGGGACAGCACAGTGCATTACAAGCACTGGTAAATATTTTGGAGAAGCCACAAAGCCTTGCAACTTTAAAGCTTACACCTAGCCAAGTGATCGAGCCTCTAATTTCATTTCTGGAATCCCCATCTCAAGCCATCCAGCAGCTTGGCACAGAATTGCTATCTCATCTTCTTGCACAAGAACATTTTCAGCAAGATATTACAACAAAAAATGCAGTTGTTCCTCTTGTTCAGCTTGCAGGAATTGGAATATTAAACTTACAGCAGACAGCGGTAAAGGCACTGGAAAAAATCTCAACAAGCTGGCCAAGGGCAGTTGCTGATGCTGGTGGTATCTTTGAGCTTGGAAAGGTTATTATTCAAGATGACCCTCAGCCACCTCATGCCCTGTGGGAATCAGCTGCTTTAGTTCTCTCAAATGTTCTGCGCTTCAACGCTGAATACTATTTTAAGGTTCCTGTGGTGGTTCTTGTTAAAATGTTGCATTCAACACTGGATAGCACCATCACGGTGGCCCTCAATGCGTTACTTGTTCACGAAAGGAATGATAATTTAAGTGCAGAACAGATGACTGATGGTGGTGCTATAGAAGCCTTGTTGGACCTTTTACGGTCTCATCAATGTGAAGAAGCTTCTGGAAGATTACTTGAAGCTCTATTTAACAATGTCAGGATACGACAAATGAAGGTCTCTCAGTATGCAATAGCACCGCTATCGCAGTATCTGTTAGATCCACAAACCAAATCGCAGTCTGGAAAACTTCTTGCTGCTCTTGCTCTGGGAGACCTTTCCCAACATGAAGGGCTTGCGAGAGCTAGAGATTCTGTTTCTGCATGTCGTGCATTGATAAGCTTGCTTGAAGAACAACCAACAGAAGAGATGAAAATGGTGTCAATTTGTGCATTGCAGAACTTTGTCATGAACAGCCGAACAAATAGGCGAGCTGTTGCTGAAGCTGGGGGCATACTGATTATTCAAGAACTACTACTGTCTCCGAATGCAGAAACTGCTGGGCAGGCAGCATTGCTTATCAAATTCTTGTTTTCTAATCACACACTCCAAGAGTATGTATCAAATGAACTTATAAGATCTTTGACAGCTGCATTAGAGAGAGAGTTGTGGACCTCAGCAACTATTAATGAAGAGGTCTTGAGAGCCTTACATATGATATTCGTCAACTTCCCAAAGCTCCATATTTCTGAAGCAGCCACTCTCTGCATTCCCAATCTGATTGGAGCTCTTAAATCTGGTAGTGACACGGCTCAGGACGTTGTTTTGGACACCCTTTCCCTGTTGAGACATTCTTGGTCAACCATGCCAATAGATATTGCAAAGTCTCAGGCTGTGATTGCTGCCGAAGCCATTCCCATCCTACAAATGCTCATGAAAACCTGTCCACCAAGTTTCCAAGAGAGAGCAGACAGCCTGTTGCATTGCTTACCAGGTTGTTTGACTGTTACAATTAAGCGGGGGAACAACCTTAAACAGGCCATGGGAGGTACAAATGCTTTTTGTCGATTGACAATAGGCAATGGCCCTCCTCGACAAACCAAGGTAATGGTTCCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000271 GO:0000902 GO:0002682 GO:0002683 GO:0003674 GO:0003824 GO:0004842 GO:0005102 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005856 GO:0005874 GO:0005881 GO:0005886 GO:0005938 GO:0005975 GO:0005976 GO:0006073 GO:0006464 GO:0006807 GO:0006950 GO:0006952 GO:0008104 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009250 GO:0009605 GO:0009607 GO:0009617 GO:0009653 GO:0009664 GO:0009826 GO:0009832 GO:0009987 GO:0010330 GO:0010383 GO:0010941 GO:0015630 GO:0016020 GO:0016043 GO:0016049 GO:0016051 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0030243 GO:0030244 GO:0030863 GO:0030981 GO:0032446 GO:0032502 GO:0032989 GO:0032991 GO:0033036 GO:0033365 GO:0033612 GO:0033692 GO:0034613 GO:0034637 GO:0034645 GO:0036211 GO:0040007 GO:0042546 GO:0042742 GO:0042981 GO:0043066 GO:0043067 GO:0043069 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043412 GO:0044036 GO:0044038 GO:0044042 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044262 GO:0044264 GO:0044267 GO:0044380 GO:0044422 GO:0044424 GO:0044430 GO:0044444 GO:0044446 GO:0044448 GO:0044464 GO:0045229 GO:0048519 GO:0048523 GO:0048580 GO:0048583 GO:0048585 GO:0048589 GO:0048856 GO:0048869 GO:0050776 GO:0050777 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051179 GO:0051239 GO:0051273 GO:0051274 GO:0051641 GO:0051704 GO:0051707 GO:0052324 GO:0052541 GO:0055028 GO:0060548 GO:0060560 GO:0065007 GO:0070589 GO:0070592 GO:0070647 GO:0070696 GO:0070727 GO:0071554 GO:0071555 GO:0071669 GO:0071704 GO:0071840 GO:0071944 GO:0072697 GO:0072698 GO:0072699 GO:0098542 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0099568 GO:0140096 GO:1901564 GO:1901576 GO:1902494 GO:1990234 GO:1990778 GO:2000026 GO:2000028 GO:2000241
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Arm C2

Protein Analysis

2048

Amino Acids

219.57

Weight (kDa)

5.34

Isoelectric Point (pI)

41.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ARM_ARMC5 PF24768 107 - 335 1.9e-06 ARMC5
ARM_ARMC5 PF24768 381 - 571 5.7e-08 ARMC5
Arm PF00514 504 - 541 2.5e-06 Armadillo/beta-catenin-like repeat
ARM_ARMC5 PF24768 621 - 780 7.5e-08 ARMC5
ARM_ARMC5 PF24768 1262 - 1401 8.9e-08 ARMC5
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1313, 5660
Acc36I ACCTGC 1 cut(s) 4444
AccB1I GGYRCC 2 cut(s) 278, 3569
AccB7I CCANNNNNTGG 1 cut(s) 3298
AccBSI CCGCTC 2 cut(s) 1601, 1757
AccII CGCG 1 cut(s) 28
AclWI GGATC 7 cut(s) 2246, 2700, 2975, 3511, 3546, 4296, 5300
AcoI YGGCCR 3 cut(s) 1485, 3300, 4880
AcsI RAATTY 8 cut(s) 1416, 1615, 2640, 2884, 2966, 3349, 3991, 5609
AcuI CTGAAG 9 cut(s) 767, 2310, 2390, 2583, 2606, 2625, 3969, 5547, 5796
AcyI GRCGYC 1 cut(s) 1859
AdeI CACNNNGTG 2 cut(s) 2072, 4258
AfiI CCNNNNNNNGG 4 cut(s) 1756, 2623, 3298, 6061
AjiI CACGTC 2 cut(s) 1333, 3403
AjnI CCWGG 8 cut(s) 240, 795, 1920, 2274, 2598, 3174, 3565, 6016
AjuI GAANNNNNNNTTGG 2 cut(s) 2745, 2777
AleI CACNNNNGTG 2 cut(s) 284, 2958
Alw21I GWGCWC 8 cut(s) 1093, 1122, 2107, 2397, 2679, 3655, 3847, 5815
AlwI GGATC 7 cut(s) 2246, 2700, 2975, 3511, 3546, 4296, 5300
AlwNI CAGNNNCTG 6 cut(s) 1483, 2228, 4344, 4895, 5299, 5582
Ama87I CYCGRG 3 cut(s) 629, 1750, 2091
ApoI RAATTY 8 cut(s) 1416, 1615, 2640, 2884, 2966, 3349, 3991, 5609
ArsI GACNNNNNNTTYG 6 cut(s) 565, 597, 1986, 2018, 2958, 2990
AseI ATTAAT 4 cut(s) 1773, 3371, 3632, 5712
Asp700I GAANNNNTTC 5 cut(s) 1416, 1638, 3994, 4761, 5937
AspA2I CCTAGG 1 cut(s) 2924
AspLEI GCGC 1 cut(s) 5004
AsuC2I CCSGG 2 cut(s) 1070, 4256
AvaI CYCGRG 3 cut(s) 629, 1750, 2091
AvaII GGWCC 4 cut(s) 1924, 4374, 5176, 5697
AvrII CCTAGG 1 cut(s) 2924
AxyI CCTNAGG 2 cut(s) 451, 1194
BaeGI GKGCMC 2 cut(s) 2356, 3574
BalI TGGCCA 3 cut(s) 1487, 3302, 4882
BanI GGYRCC 2 cut(s) 278, 3569
BanII GRGCYC 6 cut(s) 1093, 3655, 3689, 3847, 4170, 5815
BarI GAAGNNNNNNTAC 2 cut(s) 492, 524
BbsI GAAGAC 7 cut(s) 36, 141, 342, 653, 660, 2129, 4053
Bbv12I GWGCWC 8 cut(s) 1093, 1122, 2107, 2397, 2679, 3655, 3847, 5815
BbvCI CCTCAGC 3 cut(s) 1907, 4947, 5701
BceAI ACGGC 4 cut(s) 292, 1310, 3578, 5850
BcgI CGANNNNNNTGC 2 cut(s) 5271, 5305
BciT130I CCWGG 8 cut(s) 242, 797, 1922, 2276, 2600, 3176, 3567, 6018
BciVI GTATCC 2 cut(s) 3136, 5241
BclI TGATCA 2 cut(s) 469, 3454
BcnI CCSGG 2 cut(s) 1070, 4256
BfmI CTRYAG 8 cut(s) 423, 918, 2220, 2265, 2382, 4200, 4206, 5160
BfuAI ACCTGC 1 cut(s) 4444
BfuI GTATCC 2 cut(s) 3136, 5241
BglI GCCNNNNNGGC 2 cut(s) 986, 3562
BglII AGATCT 1 cut(s) 5663
BlnI CCTAGG 1 cut(s) 2924
BmcAI AGTACT 3 cut(s) 354, 2310, 2823
Bme18I GGWCC 4 cut(s) 1924, 4374, 5176, 5697
BmeT110I CYCGRG 3 cut(s) 629, 1750, 2091
BmgBI CACGTC 2 cut(s) 1333, 3403
BmiI GGNNCC 7 cut(s) 280, 1666, 3037, 3571, 4376, 5031, 6138
BmrI ACTGGG 5 cut(s) 682, 873, 2321, 3286, 4440
BmuI ACTGGG 5 cut(s) 682, 873, 2321, 3286, 4440
BoxI GACNNNNGTC 1 cut(s) 342
BpiI GAAGAC 7 cut(s) 36, 141, 342, 653, 660, 2129, 4053
BpmI CTGGAG 5 cut(s) 783, 1249, 3669, 4058, 4236
Bpu10I CCTNAGC 5 cut(s) 1728, 1907, 4947, 5701, 5838
BpuEI CTTGAG 7 cut(s) 1247, 1935, 3104, 3565, 4445, 4689, 5746
BpuMI CCSGG 2 cut(s) 1070, 4256
BsaAI YACGTR 1 cut(s) 3515
BsaBI GATNNNNATC 1 cut(s) 2704
BsaHI GRCGYC 1 cut(s) 1859
BsaI GGTCTC 5 cut(s) 1917, 3541, 5193, 5269, 5352
BsaWI WCCGGW 1 cut(s) 4371
BsaXI ACNNNNNCTCC 4 cut(s) 1247, 1277, 4031, 4061
Bsc4I CCNNNNNNNGG 4 cut(s) 1756, 2623, 3298, 6061
Bse118I RCCGGY 2 cut(s) 1535, 3074
Bse21I CCTNAGG 2 cut(s) 451, 1194
Bse3DI GCAATG 8 cut(s) 87, 252, 2820, 4275, 5477, 5597, 6007, 6112
Bse8I GATNNNNATC 1 cut(s) 2704
BseBI CCWGG 8 cut(s) 242, 797, 1922, 2276, 2600, 3176, 3567, 6018
BseJI GATNNNNATC 1 cut(s) 2704
BseLI CCNNNNNNNGG 4 cut(s) 1756, 2623, 3298, 6061
BseMI GCAATG 8 cut(s) 87, 252, 2820, 4275, 5477, 5597, 6007, 6112
BseRI GAGGAG 7 cut(s) 7, 1539, 1784, 2781, 2887, 4099, 6105
BseSI GKGCMC 2 cut(s) 2356, 3574
BseYI CCCAGC 3 cut(s) 1672, 5531, 5585
BsgI GTGCAG 8 cut(s) 1152, 1680, 2214, 2808, 4080, 4425, 4509, 5154
Bsh1236I CGCG 1 cut(s) 28
BshNI GGYRCC 2 cut(s) 278, 3569
BsiHKAI GWGCWC 8 cut(s) 1093, 1122, 2107, 2397, 2679, 3655, 3847, 5815
BsiHKCI CYCGRG 3 cut(s) 629, 1750, 2091
BsiSI CCGG 5 cut(s) 1070, 1536, 3075, 4255, 4372
BslFI GGGAC 5 cut(s) 1733, 2095, 2482, 2960, 4596
BslI CCNNNNNNNGG 4 cut(s) 1756, 2623, 3298, 6061
BsmBI CGTCTC 2 cut(s) 722, 1562
BsmFI GGGAC 5 cut(s) 1733, 2095, 2482, 2960, 4596
Bso31I GGTCTC 5 cut(s) 1917, 3541, 5193, 5269, 5352
BsoBI CYCGRG 3 cut(s) 629, 1750, 2091
Bsp1407I TGTACA 1 cut(s) 4047
Bsp19I CCATGG 3 cut(s) 1233, 2546, 6066
BspFNI CGCG 1 cut(s) 28
BspHI TCATGA 3 cut(s) 3850, 5493, 5958
BspLI GGNNCC 7 cut(s) 280, 1666, 3037, 3571, 4376, 5031, 6138
BspMAI CTGCAG 3 cut(s) 427, 2224, 2269
BspMI ACCTGC 1 cut(s) 4444
BspPI GGATC 7 cut(s) 2246, 2700, 2975, 3511, 3546, 4296, 5300
BspQI GCTCTTC 4 cut(s) 972, 3252, 3852, 4465
BspT107I GGYRCC 2 cut(s) 278, 3569
BspTNI GGTCTC 5 cut(s) 1917, 3541, 5193, 5269, 5352
BsrBI CCGCTC 2 cut(s) 1601, 1757
BsrDI GCAATG 8 cut(s) 87, 252, 2820, 4275, 5477, 5597, 6007, 6112
BsrFI RCCGGY 2 cut(s) 1535, 3074
BsrGI TGTACA 1 cut(s) 4047
BssAI RCCGGY 2 cut(s) 1535, 3074
BssNI GRCGYC 1 cut(s) 1859
BssT1I CCWWGG 8 cut(s) 1233, 2546, 2710, 2924, 4074, 4883, 6066, 6126
Bst2UI CCWGG 8 cut(s) 242, 797, 1922, 2276, 2600, 3176, 3567, 6018
Bst6I CTCTTC 7 cut(s) 972, 3252, 3423, 3852, 4465, 5445, 5712
BstACI GRCGYC 1 cut(s) 1859
BstAPI GCANNNNNTGC 6 cut(s) 113, 2102, 2228, 2351, 5414, 5582
BstAUI TGTACA 1 cut(s) 4047
BstBAI YACGTR 1 cut(s) 3515
BstDSI CCRYGG 4 cut(s) 1233, 2546, 4464, 6066
BstENI CCTNNNNNAGG 1 cut(s) 6059
BstFNI CGCG 1 cut(s) 28
BstHHI GCGC 1 cut(s) 5004
BstNI CCWGG 8 cut(s) 242, 797, 1922, 2276, 2600, 3176, 3567, 6018
BstNSI RCATGY 8 cut(s) 140, 148, 946, 1650, 3111, 3925, 4324, 5412
BstPAI GACNNNNGTC 1 cut(s) 342
BstSFI CTRYAG 8 cut(s) 423, 918, 2220, 2265, 2382, 4200, 4206, 5160
BstSLI GKGCMC 2 cut(s) 2356, 3574
BstUI CGCG 1 cut(s) 28
BstV2I GAAGAC 7 cut(s) 36, 141, 342, 653, 660, 2129, 4053
BstX2I RGATCY 5 cut(s) 2705, 3538, 4288, 5305, 5663
BstXI CCANNNNNNTGG 4 cut(s) 519, 4721, 5086, 6133
BstYI RGATCY 5 cut(s) 2705, 3538, 4288, 5305, 5663
Bsu36I CCTNAGG 2 cut(s) 451, 1194
BsuI GTATCC 2 cut(s) 3136, 5241
BtgI CCRYGG 4 cut(s) 1233, 2546, 4464, 6066
BtrI CACGTC 2 cut(s) 1333, 3403
BtsI GCAGTG 1 cut(s) 152
BveI ACCTGC 1 cut(s) 4444
CaiI CAGNNNCTG 6 cut(s) 1483, 2228, 4344, 4895, 5299, 5582
CciI TCATGA 3 cut(s) 3850, 5493, 5958
CfoI GCGC 1 cut(s) 5004
Cfr10I RCCGGY 2 cut(s) 1535, 3074
CseI GACGC 5 cut(s) 332, 1848, 1953, 3794, 3872
CsiI ACCWGGT 1 cut(s) 6016
DraI TTTAAA 4 cut(s) 69, 1692, 2844, 4647
DraIII CACNNNGTG 2 cut(s) 2072, 4258
EaeI YGGCCR 3 cut(s) 1485, 3300, 4880
Eam1104I CTCTTC 7 cut(s) 972, 3252, 3423, 3852, 4465, 5445, 5712
EarI CTCTTC 7 cut(s) 972, 3252, 3423, 3852, 4465, 5445, 5712
Ecl136II GAGCTC 4 cut(s) 1091, 3653, 3845, 5813
Eco130I CCWWGG 8 cut(s) 1233, 2546, 2710, 2924, 4074, 4883, 6066, 6126
Eco24I GRGCYC 6 cut(s) 1093, 3655, 3689, 3847, 4170, 5815
Eco31I GGTCTC 5 cut(s) 1917, 3541, 5193, 5269, 5352
Eco32I GATATC 2 cut(s) 649, 3751
Eco47I GGWCC 4 cut(s) 1924, 4374, 5176, 5697
Eco53kI GAGCTC 4 cut(s) 1091, 3653, 3845, 5813
Eco57I CTGAAG 9 cut(s) 767, 2310, 2390, 2583, 2606, 2625, 3969, 5547, 5796
Eco81I CCTNAGG 2 cut(s) 451, 1194
Eco88I CYCGRG 3 cut(s) 629, 1750, 2091
EcoICRI GAGCTC 4 cut(s) 1091, 3653, 3845, 5813
EcoNI CCTNNNNNAGG 1 cut(s) 6059
EcoO109I RGGNCCY 2 cut(s) 1924, 2609
EcoRI GAATTC 1 cut(s) 1416
EcoRII CCWGG 8 cut(s) 240, 795, 1920, 2274, 2598, 3174, 3565, 6016
EcoRV GATATC 2 cut(s) 649, 3751
EcoT14I CCWWGG 8 cut(s) 1233, 2546, 2710, 2924, 4074, 4883, 6066, 6126
EcoT22I ATGCAT 4 cut(s) 2347, 2455, 3029, 4188
EcoT38I GRGCYC 6 cut(s) 1093, 3655, 3689, 3847, 4170, 5815
ErhI CCWWGG 8 cut(s) 1233, 2546, 2710, 2924, 4074, 4883, 6066, 6126
Esp3I CGTCTC 2 cut(s) 722, 1562
FalI AAGNNNNNCTT 4 cut(s) 765, 797, 1329, 1361
FaqI GGGAC 5 cut(s) 1733, 2095, 2482, 2960, 4596
FauI CCCGC 3 cut(s) 356, 2120, 6036
FauNDI CATATG 1 cut(s) 5739
FbaI TGATCA 2 cut(s) 469, 3454
FriOI GRGCYC 6 cut(s) 1093, 3655, 3689, 3847, 4170, 5815
GlaI GCGC 1 cut(s) 5003
GsaI CCCAGC 3 cut(s) 1676, 5535, 5589
GsuI CTGGAG 5 cut(s) 783, 1249, 3669, 4058, 4236
HapII CCGG 5 cut(s) 1070, 1536, 3075, 4255, 4372
HgaI GACGC 5 cut(s) 332, 1848, 1953, 3794, 3872
HhaI GCGC 1 cut(s) 5004
Hin1I GRCGYC 1 cut(s) 1859
Hin6I GCGC 1 cut(s) 5002
HinP1I GCGC 1 cut(s) 5002
HincII GTYRAC 6 cut(s) 861, 1330, 3919, 3981, 5752, 5886
HindII GTYRAC 6 cut(s) 861, 1330, 3919, 3981, 5752, 5886
HindIII AAGCTT 9 cut(s) 774, 1205, 1363, 1742, 2575, 3101, 4649, 5205, 5425
HpaII CCGG 5 cut(s) 1070, 1536, 3075, 4255, 4372
Hpy99I CGWCG 1 cut(s) 3404
HpyCH4IV ACGT 6 cut(s) 181, 1332, 3402, 3514, 4561, 5844
HpySE526I ACGT 6 cut(s) 181, 1332, 3402, 3514, 4561, 5844
Hsp92I GRCGYC 1 cut(s) 1859
HspAI GCGC 1 cut(s) 5002
Ksp22I TGATCA 2 cut(s) 469, 3454
LguI GCTCTTC 4 cut(s) 972, 3252, 3852, 4465
MabI ACCWGGT 1 cut(s) 6016
MaeII ACGT 6 cut(s) 181, 1332, 3402, 3514, 4561, 5844
MbiI CCGCTC 2 cut(s) 1601, 1757
MfeI CAATTG 4 cut(s) 405, 1778, 4146, 4503
MflI RGATCY 5 cut(s) 2705, 3538, 4288, 5305, 5663
MlsI TGGCCA 3 cut(s) 1487, 3302, 4882
MluNI TGGCCA 3 cut(s) 1487, 3302, 4882
MlyI GAGTC 4 cut(s) 1403, 1796, 3404, 3689
MmeI TCCRAC 6 cut(s) 2743, 3423, 3481, 4221, 4279, 5154
Mox20I TGGCCA 3 cut(s) 1487, 3302, 4882
Mph1103I ATGCAT 4 cut(s) 2347, 2455, 3029, 4188
MroXI GAANNNNTTC 5 cut(s) 1416, 1638, 3994, 4761, 5937
MscI TGGCCA 3 cut(s) 1487, 3302, 4882
MslI CAYNNNNRTG 7 cut(s) 284, 1106, 1681, 2958, 3065, 5084, 5196
Msp20I TGGCCA 3 cut(s) 1487, 3302, 4882
MspA1I CMGCKG 5 cut(s) 1532, 4205, 4847, 4976, 5675
MspI CCGG 5 cut(s) 1070, 1536, 3075, 4255, 4372
MunI CAATTG 4 cut(s) 405, 1778, 4146, 4503
MvaI CCWGG 8 cut(s) 242, 797, 1922, 2276, 2600, 3176, 3567, 6018
MvnI CGCG 1 cut(s) 28
NciI CCSGG 2 cut(s) 1070, 4256
NcoI CCATGG 3 cut(s) 1233, 2546, 6066
NdeI CATATG 1 cut(s) 5739
NlaIV GGNNCC 7 cut(s) 280, 1666, 3037, 3571, 4376, 5031, 6138
NsiI ATGCAT 4 cut(s) 2347, 2455, 3029, 4188
NspI RCATGY 8 cut(s) 140, 148, 946, 1650, 3111, 3925, 4324, 5412
OliI CACNNNNGTG 2 cut(s) 284, 2958
PacI TTAATTAA 1 cut(s) 3975
PaeI GCATGC 2 cut(s) 946, 4324
PaeR7I CTCGAG 2 cut(s) 629, 2091
PagI TCATGA 3 cut(s) 3850, 5493, 5958
PciSI GCTCTTC 4 cut(s) 972, 3252, 3852, 4465
PdmI GAANNNNTTC 5 cut(s) 1416, 1638, 3994, 4761, 5937
PflMI CCANNNNNTGG 1 cut(s) 3298
PleI GAGTC 4 cut(s) 1402, 1796, 3403, 3689
PpsI GAGTC 4 cut(s) 1402, 1796, 3403, 3689
Ppu21I YACGTR 1 cut(s) 3515
PpuMI RGGWCCY 1 cut(s) 1924
PshAI GACNNNNGTC 1 cut(s) 342
PshBI ATTAAT 4 cut(s) 1773, 3371, 3632, 5712
PsiI TTATAA 2 cut(s) 1313, 5660
Psp124BI GAGCTC 4 cut(s) 1093, 3655, 3847, 5815
Psp5II RGGWCCY 1 cut(s) 1924
Psp6I CCWGG 8 cut(s) 240, 795, 1920, 2274, 2598, 3174, 3565, 6016
PspFI CCCAGC 3 cut(s) 1672, 5531, 5585
PspGI CCWGG 8 cut(s) 240, 795, 1920, 2274, 2598, 3174, 3565, 6016
PspN4I GGNNCC 7 cut(s) 280, 1666, 3037, 3571, 4376, 5031, 6138
PspPPI RGGWCCY 1 cut(s) 1924
PspXI VCTCGAGB 2 cut(s) 629, 2091
PsrI GAACNNNNNNTAC 2 cut(s) 2522, 2554
PstI CTGCAG 3 cut(s) 427, 2224, 2269
PstNI CAGNNNCTG 6 cut(s) 1483, 2228, 4344, 4895, 5299, 5582
PsuI RGATCY 5 cut(s) 2705, 3538, 4288, 5305, 5663
PvuII CAGCTG 4 cut(s) 1532, 4205, 4976, 5675
RseI CAYNNNNRTG 7 cut(s) 284, 1106, 1681, 2958, 3065, 5084, 5196
SacI GAGCTC 4 cut(s) 1093, 3655, 3847, 5815
SapI GCTCTTC 4 cut(s) 972, 3252, 3852, 4465
ScaI AGTACT 3 cut(s) 354, 2310, 2823
SchI GAGTC 4 cut(s) 1403, 1796, 3404, 3689
SexAI ACCWGGT 1 cut(s) 6016
SfcI CTRYAG 8 cut(s) 423, 918, 2220, 2265, 2382, 4200, 4206, 5160
Sfr274I CTCGAG 2 cut(s) 629, 2091
SinI GGWCC 4 cut(s) 1924, 4374, 5176, 5697
SlaI CTCGAG 2 cut(s) 629, 2091
SmiI ATTTAAAT 1 cut(s) 69
SmiMI CAYNNNNRTG 7 cut(s) 284, 1106, 1681, 2958, 3065, 5084, 5196
SmlI CTYRAG 9 cut(s) 629, 1226, 1950, 2091, 3083, 3544, 4424, 4704, 5725
SmoI CTYRAG 9 cut(s) 629, 1226, 1950, 2091, 3083, 3544, 4424, 4704, 5725
SphI GCATGC 2 cut(s) 946, 4324
SspI AATATT 4 cut(s) 489, 3732, 4615, 4827
SstI GAGCTC 4 cut(s) 1093, 3655, 3847, 5815
StyI CCWWGG 8 cut(s) 1233, 2546, 2710, 2924, 4074, 4883, 6066, 6126
SwaI ATTTAAAT 1 cut(s) 69
TaiI ACGT 6 cut(s) 184, 1335, 3405, 3517, 4564, 5847
TaqI TCGA 6 cut(s) 630, 1814, 2092, 4671, 6091, 6118
TatI WGTACW 6 cut(s) 352, 2308, 2799, 2821, 4047, 4109
TauI GCSGC 1 cut(s) 1601
TspGWI ACGGA 3 cut(s) 806, 3161, 4453
Van91I CCANNNNNTGG 1 cut(s) 3298
VpaK11BI GGWCC 4 cut(s) 1924, 4374, 5176, 5697
VspI ATTAAT 4 cut(s) 1773, 3371, 3632, 5712
XagI CCTNNNNNAGG 1 cut(s) 6059
XapI RAATTY 8 cut(s) 1416, 1615, 2640, 2884, 2966, 3349, 3991, 5609
XbaI TCTAGA 2 cut(s) 2749, 2902
XceI RCATGY 8 cut(s) 140, 148, 946, 1650, 3111, 3925, 4324, 5412
XcmI CCANNNNNNNNNTGG 1 cut(s) 1680
XhoI CTCGAG 2 cut(s) 629, 2091
XmaJI CCTAGG 1 cut(s) 2924
XmnI GAANNNNTTC 5 cut(s) 1416, 1638, 3994, 4761, 5937
ZrmI AGTACT 3 cut(s) 354, 2310, 2823
Zsp2I ATGCAT 4 cut(s) 2347, 2455, 3029, 4188
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.