FvH4_2g27420

Protein kinase C conserved region 2 (CalB)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0009147
Physical Location & Seq
Reverse (-)
21771207 .. 21780638
9432 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g27420.t1

Sequence Viewer

Length: 6405 bp
ATGTCGAAGTCTTCCTCCCCTGAACTACGGGAACCCACGTCTTCTTCTACTTCTCGGTCAAGGGATTTAGATGAAACAATGGATGATGAAGAAGGTACAATGGCAAGTGTTGCTCAGTTTGTTGAGCAACTACATGCCAGCATGTCTTCACCACAAGAAAAAGAACATATAACAGCACGTCTGCTAGGTATTGCCAAAGCAAGGAAGGATGCAAGAACAATCATTGGTTCACATTCCCAGGCTATGCCATTGTTCATAAATATTCTCAGGAGCGGCACCCCTGTTGCAAAGGTTAATGTTGCGGCAACTCTCAGTGTTCTGTGTAAAGATGGAGACTTGCGTCTTAAAGTACTTCTAGGTGGGTGCATCCCCCCGTTACTCTCACTTCTGAAGTCGGAGTCAACTGAGGCCAGGAAGGCTGCGGCTGAAGCTATATATGAAGTTTCTTCCGGAGGGCTTTCAGATGATCATGTTGGCATGAAAATATTTATTACAGAAGGTGTTGTACCAAACTTGTGGAATCAACTGAATCCCAACAGCAAACAGGACAAAGTGGTAGAGGGATTTGTGACAGGGGCTTTAAGAAATCTTTGTGGCGACAAGGATGGTTACTGGAGAGCAACACTTGAGGCTGGTGGAGTAGATATCACTGTAGGTCTCCTGTATTCTGACAATGCGGCCGCCCAGTCCAATGCTGCGTCCCTTTTGGCCCGTCTCATGCTGGCTTTCAGTGATAGCATCCCCAAAGTAATAGATTCTGGAGCTGTCAAAGCTTTGCTTCAGCTTGTGGGTCAAGAAAATGATGTTTCTGTTCGTGCTAGTGCTGCTGATGCTCTGGAAGCTCTTTCTTCAAAGTCAACTGGCGCTAAGAAAGCTATAGTTGATGCAAATGGTTTTCCAGTTCTGATTGGGGCTATTGTTGCTCCATCCAAGGAGTGCATGCGAGGTGAGTGTGGGCAGGCTCTCCAGGAGCATTCAACAAGGGCTTTAGCAAATATTTGTGGTGGGGTTTCGGCTTTAATACTTTATCTTGGAGAGCTTTCACAGTCAGCTCGCCTATCTGCCCCAGTTGCTGATATCATTGGAGCTCTTGCGTACACTTTAATGGTCTTTGAGCAAAAATCTGGTGATGGTAAGGAATCTTTTAAGGTAACAAAGATAGAGGACATTCTCGTAATGCTGCTAAAGCCTCGTGATAATAAACTTGTTCAAGAGCGTGTCCTCGAGGCCATGGCTAGTCTGTATGGCAACATTCATCTCTCAAAATGGCTCAATCATGCAGAAGCGAAGAAGGTGCTTATTGGACTTATAACAATGGCTGCTGCCGATGTACAAGAGTCTCTAATACTGTCTTTGACAAGCTTATGCTGTGATGGTGTTGGAATCTGGGAGTCCATTGGCGAGAGAGAAGGAATTCAGCTACTTATTTCATTGCTTGGTTTATCCAGTGAGCAACATCAAGAATATGCTGTTCAATTGCTGGGGATCTTAACTGACCAGGTTGATGACAGCAAGTGGGCTATTACTGCTGCTGGTGGGATTCCTCCTCTAGTGCAGTTGTTAGAAACGGGGTCTCAAAAGGCGAAGGAGGATGCCGCTCATGTGTTGTGGAATTTGTGCTGTCACAGTGAGGATATTCGTGCATGTGTTGAAAGTGCAGGAGCCATTCCTGCATTTTTATGGCTTCTAAAAAGTGGGGGAACAAAAGGGCAAGAAGCATCAGCTATGGCACTTACAAAGCTTATCCGAACAGCCGATTCTGCCACCATTAATCAGTTGTTAGCTTTGCTCCTAGGTGACTCTCCATGCTCAAAGGCCCATACTATCACGGTGTTGGGTCATGTACTCATAATGGCATCACACAAGGATCTTGTGCATAAGGGTTCAGCAGCTAATAAAGGGTTGAGGTCTCTCGTTCAGGTTCTCAACTCATCAAATGAAGAAACTCAAGAGTATGCTGCGTCTGTCTTAGCTGATCTATTCAGCACAAGACAAGATATTTGCGATACTCTAGCAACTGATGAGATTGTGCATCCTTGCATGAAGCTTTTGACCAGCAGTACGCAAGCTGTTGCAACACAGTCAGCTCGAGCTCTGGGTGCTCTATCCCGTCCTATGAAGACCAAAACTATAAGCAAGATGTCGTATATTGCAGAAGGGGATGTTAAACCCCTCATTAAACTAGCGAAAACTTCTTCAATTGATGCTGCTCAAACTGCAGTTGCTGCACTTGCAAATCTTCTCTCTGATCCCCAGATTGCTGCCGAAGCCCTGGCAGAAGATGTTGTTTCAGCTTTGATAAGGGTACTGGGAGATGGAACTACAGAAGGTAAAAAGAATGCATCCCGTGCCCTCCATCAATTGTTGAAGCATTTTCCAGTAGGTGATGTGCTCACAGGAAATGCTCATTGCCGTTTTGCTATTCTTGCTGTTGTTGATTCCTTAAATGCATTGGATATGGATGAGACTGATGCTGCTGATGCTTTAGAAGTTGTAGCACTTTTGGCCAGAACTAAAATGGGTGCGAATTTCACTTACCCTCCATGGTCTGTATTTGCTGAAGTTGAAGCAAGCTTAGAACCTCTTGTCCGCTGTCTGGCCGAAGGGCCTCCTTTGCTTCAGGATAAGGCGATAGAAATTTTGTCTAGGCTTTGTGGGGAGCAACCAGTTGTGCTCGGTGATCTATTGGTAGCAAGATCTAGATCCCTTGGTTCACTAGCTAACAGAATAATGAACTCATCTAGTTTAGAAGTCAGAGTTGGAGGGGCTGCATTACTCATTTGTGCAGCAAAAGAGCACAAAGAGCAGTGGATGGAAGTACTTGAGGTGTCAGGATGTTTAAAACCACTAATGTATGCTTTAGTGGATATGATGAAGCAAAATTCTAGCTGCTCCTCTTTAGAAATTGAAGTCCGAACTTCTAAAGCTTTTATGGAAAGATCTGCATTCCACGAAGGGGATGAGTTTAATGTCCCTGATCCAGCTGTTGTCTTGGCCGGTACAGTTGCTTTGTGGTTGCTATGTATAATTGGTTCCTGTAATGCAAAGAGCAAACTCACCATTATGGAAGCGGGTGGTCTTGAGGCTCTCTCTGACAAACTTGAGAGCCACACTTCCAATCCACAGGCAGAATATGAGGATACTGAAGGTATATGGATTAGTGCCCTGCTACTGGCTATTCTCTTCCAAGATGCCAATGTTGTTTCATCTCCTGCAACAATGCGCATCATAGCTTCACTTGCGCTTCTGCTGAGATCGGATGAGGTGATTGATAGGTTCTTTGCTGCCCAGTCAATGGCCAGTCTTGTTTGCAGTGGTAATAAGGAAACAATTCTTGCTATTGCAAATTCAGGTGCTGTTGCTGGTTTGATAACCCTCATTGGTTTTGTAGAATCCGATATGCCTAACCTTGTTACTTTATCACAAGAGTTTTCTCTGATGCGAAACCCTGATCAAGTTGTCTTGGAGCACCTTTTTGATTTTGAAGATGTAAGAGTTGGATCCACAGCACGTAAATCTATACCGCTCTTGGTGGATCTCTTGAGACCAATGCCAGATAGACCTGGTGCCCCCCCAGTTGCTCTTAAGCTCTTGACCTGTATCGCTGATGGAAGTGATACAAATAAATTAGTCATGGCTGAAGCTGGAGCTCTGGATGCTCTGACAAAGTACCTGTCTTTGAGCCCACAAGATTCGACTGAAGCTGCAATATCTGATTTATTCAGAATATTGTTTAGCCATCCTGATCTGATTCGTTATGAAGCATCAGCTAGTTCATTGAATCAACTCATAGCCGTTCTGCGTCTGGGGTCAAGAAATGCTAGATACAGTGCTGCGAGAGCTCTTCACGAACTATTTGATGCTGAGAACATCAGAGATTCTGATTTAGCCAGGCAATCTGTTCAGCCTTTGGTTGACATGCTTAACGCTGCATCGGAGAATGAACAGGAGGCTGCTCTTGTTGCTATAATTAAGCTGACTTCAGGGAACTCTTATACCGCAGCTTTGTTGACTGACGTGGAGGGAAATCCACTGGAGAGTTTATTTAAAATACTTTCTTCAGCTGCATCCTTGGATTTAAAGAGACTTGCTGCACAATTATGTTGTGTACTGTTTGATAATACCCAAGTCAGAGGAAATCCAATTGCCTCTGAATGTATAGAGCCTCTTATTTCCTTGATGCTTTCTGGTATAAATGCAGCTGTAGAAGCTGGGGTTTGTGCTTTTGAAAAATTGTTGGATGATGAACACCAGGTAGAGCTTGCAGTGAACTATAATGTTGTCAATCTCCTTGTTGGATTAGTTTCTGGTACGAACAGTCAGCTCATTGAGGCAAGCATCTGCTCTCTCATAAAGTTGGGCAAAGACCGAACCCGATGCAAATCAGATATGATCAATGCTGGCATTATTGATAAGTGTCTTGAGCTACTTCCTGTTGCAGCCAGTTCATTATGCTCGTCTATTGCTGAATTATTTCGCATTTTAACAAATAGTGATGCAATTGCTAGAAGTTTGGCTGCTGCAACAATCGTAGAACCCCTTTTCCTTGTTTTACTTCGTCCAGATTTCAGTATGTGGGGACAGCACAGTGCATTGCAAGCTCTAGTAAATATTTTGGAGAAGCCACAAAGCCTCGCAACTTTGAAACTTACGCCTAGCCAAGTCATTGAGCCTCTGATTTCATTTCTTGAATCCCCATCTCTAGCCATCCAGCAGCTTGGTACAGAATTGCTATCTCATCTTCTTGCACAAGAACATTTTCAGCAAGATATTACAACAAAAAATGCAGTTGTTCCTCTAGTGCAACTTGCTGGAATTGGAATATTGAACTTACAGCAAACAGCGATAATGGCATTGGAAAAAATCTCTACAAGCTGGCCAAAGGCAGTTGCTGATGCTGGAGGTATTTTTGAGCTCGGAAAGGTTATCATACAAGATGATCCTCAGCCACCTCATGCATTGTGGGAATCAGCTGCCTTAGTTCTCTCAAATGTTTTGTGCTTCAATGCTGAGTACTACTTTAAGGTTCCTGTGGTGGTTCTTGTTAAAATGTTGCATTCAACGGTGGATAGCACTATTACTGTGGCTCTCAATGCACTACTTGTTCATGAAAGGAGTGATAAGCTAAGTGCTGAACAGATGACTGAAAATGGTGTTATAGATGCCTTGTTGGACCTTTTAAGATCTCATCAATGTGAAGAAGCATCTGGAAGGTTACTTGAAGCTTTATTCAACAATGCCAGGATACGAGCAATGAAGGTTTCCAAGTATGCAATAGCACCTTTATCACAGTATCTGTTGGATTCACAAACCAAATCACAATCTGGAAAACTTCTCGTAGCTCTAGCTCTTGGAGACCTATCCCAACATGAAGGGCTCGCTAGAGCCAGTGATTCTGTTTCTGCATGTCGTGCATTAGTAAGCTTGCTTGAAGATCAGCCCACAGAAGATATGAAAATGGTGGCAATTTGTGCATTGCAAAATTTTGTCATGAATAGCAGAACGAATCGGCGTGCTGTCGCAGAAGCAGGGGGCATTCTGGTTATTCAAGAACTACTACTATCTCTCAACACAGAAGTTGCTGGGCAGGCAGCATTGCTTATCAAATTTTTGTTTTCTAATCACACACTTCAAGAATATGTATCGAACGAGCTCATCAGATCTCTGACAGCTGCATTAGAGAGAGAGCTGTGGTCTGCAGCAACTATTAATGAAGAGGTCTTGAGAGCCCTGCATATGATATTCACCAACTTTCCTAAGCTCCATATTTCTGAGGCAGCCACTCTCTGCATTCCCAATCTGATTGGCGCTCTTAAATCTGGCAGTGAGGCAGCTCAGGATGTCGTGTTGGACACCCTATCCTTGTTGAAACATTCTTGGTCAACCATGCCAATAGATATTGCGAAGTCTCAGGCCGTGGTTGCTGCTGAAGCTATTCCCATCTTACAAATGCTTATGAAAACCTGCCCACCAAGTTTCCATGAGAGGGCAGACAGCCTGTTGCATTGCTTACCAGGTTGTTTGACTGTTACAATCAAGCGTGGTAACAACCTTAAACAGGCTATGGGAGGCACAAATGCTTTTTGCCGATTGACAATAGGCAATGGTCCTCCTCGACAAACCAAGGTAGTGAGCCATAGTACGTCTCCCGAATGGAAAGAAGGGTTTACGTGGGCATTTGATGTGCCCCCAAAGGGACAAAAGCTCCATATCATCTGCAAAAGCAAAAATACCTTTGGAAAGTCAACACTGGGGAGAGTGACTATCCAGATTGATAAGGTTGTGTCGGAAGGAGTATACAGTGGGTTATTCAGCCTAAACCATGACAACAACAAAGATGGATCTTCGCGTACACTTGAGATCGAGATTATCTGGTCCAACAGAATGCCTGATGAGGACATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000271 GO:0000902 GO:0002682 GO:0002683 GO:0003674 GO:0003824 GO:0004842 GO:0005102 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005856 GO:0005874 GO:0005881 GO:0005886 GO:0005938 GO:0005975 GO:0005976 GO:0006073 GO:0006464 GO:0006807 GO:0006950 GO:0006952 GO:0008104 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009250 GO:0009605 GO:0009607 GO:0009617 GO:0009653 GO:0009664 GO:0009826 GO:0009832 GO:0009987 GO:0010330 GO:0010383 GO:0010941 GO:0015630 GO:0016020 GO:0016043 GO:0016049 GO:0016051 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0030243 GO:0030244 GO:0030863 GO:0030981 GO:0032446 GO:0032502 GO:0032989 GO:0032991 GO:0033036 GO:0033365 GO:0033612 GO:0033692 GO:0034613 GO:0034637 GO:0034645 GO:0036211 GO:0040007 GO:0042546 GO:0042742 GO:0042981 GO:0043066 GO:0043067 GO:0043069 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043412 GO:0044036 GO:0044038 GO:0044042 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044262 GO:0044264 GO:0044267 GO:0044380 GO:0044422 GO:0044424 GO:0044430 GO:0044444 GO:0044446 GO:0044448 GO:0044464 GO:0045229 GO:0048519 GO:0048523 GO:0048580 GO:0048583 GO:0048585 GO:0048589 GO:0048856 GO:0048869 GO:0050776 GO:0050777 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051179 GO:0051239 GO:0051273 GO:0051274 GO:0051641 GO:0051704 GO:0051707 GO:0052324 GO:0052541 GO:0055028 GO:0060548 GO:0060560 GO:0065007 GO:0070589 GO:0070592 GO:0070647 GO:0070696 GO:0070727 GO:0071554 GO:0071555 GO:0071669 GO:0071704 GO:0071840 GO:0071944 GO:0072697 GO:0072698 GO:0072699 GO:0098542 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0099568 GO:0140096 GO:1901564 GO:1901576 GO:1902494 GO:1990234 GO:1990778 GO:2000026 GO:2000028 GO:2000241
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Arm C2

Protein Analysis

2135

Amino Acids

228.97

Weight (kDa)

5.28

Isoelectric Point (pI)

38.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ARM_PUB PF25598 64 - 287 1e-05 Plant U-box protein ARM repeat region
ARM_ARMC5 PF24768 379 - 572 1.7e-07 ARMC5
Arm PF00514 503 - 540 2.6e-06 Armadillo/beta-catenin-like repeat
ARM_ARMC5 PF24768 633 - 784 4.4e-08 ARMC5
C2 PF00168 2007 - 2105 5.7e-13 C2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1310
AbsI CCTCGAGG 1 cut(s) 1223
Acc16I TGCGCA 1 cut(s) 3224
Acc36I ACCTGC 1 cut(s) 5972
AccB1I GGYRCC 2 cut(s) 275, 3566
AccB7I CCANNNNNTGG 1 cut(s) 3295
AccBSI CCGCTC 3 cut(s) 273, 1598, 3526
AccI GTMKAC 1 cut(s) 6297
AccII CGCG 1 cut(s) 6349
AccIII TCCGGA 1 cut(s) 449
AcoI YGGCCR 6 cut(s) 678, 2505, 2598, 2994, 3297, 4877
AcsI RAATTY 8 cut(s) 1413, 1612, 2527, 2637, 2881, 3346, 5482, 5606
AfiI CCNNNNNNNGG 8 cut(s) 201, 2596, 2956, 3172, 3295, 5986, 6058, 6194
AflII CTTAAG 1 cut(s) 3584
AflIII ACRYGT 1 cut(s) 6399
AjiI CACGTC 3 cut(s) 39, 179, 4018
AjuI GAANNNNNNNTTGG 2 cut(s) 2742, 2774
AloI GAACNNNNNNTCC 4 cut(s) 2571, 2603, 4527, 4559
AlwNI CAGNNNCTG 5 cut(s) 1073, 2225, 3356, 4892, 5296
Ama87I CYCGRG 2 cut(s) 1223, 2088
Aor13HI TCCGGA 1 cut(s) 449
ApoI RAATTY 8 cut(s) 1413, 1612, 2527, 2637, 2881, 3346, 5482, 5606
ArsI GACNNNNNNTTYG 4 cut(s) 1983, 2015, 3659, 3691
AseI ATTAAT 2 cut(s) 1770, 5709
Asp700I GAANNNNTTC 5 cut(s) 1413, 3330, 4473, 4758, 5934
AspA2I CCTAGG 1 cut(s) 1792
AspLEI GCGC 4 cut(s) 866, 3225, 3244, 5810
AspS9I GGNCC 6 cut(s) 709, 1816, 2606, 5173, 6107, 6375
AsuHPI GGTGA 9 cut(s) 141, 959, 1139, 1808, 2396, 2690, 3049, 3277, 5737
AvaI CYCGRG 2 cut(s) 1223, 2088
AvaII GGWCC 3 cut(s) 5173, 6107, 6375
AvrII CCTAGG 1 cut(s) 1792
BaeGI GKGCMC 4 cut(s) 2353, 3166, 3571, 6189
BalI TGGCCA 3 cut(s) 2507, 3299, 4879
BamHI GGATCC 1 cut(s) 3500
BanI GGYRCC 2 cut(s) 275, 3566
BanII GRGCYC 9 cut(s) 1090, 2095, 3652, 3686, 3844, 4917, 5379, 5655, 5731
BarI GAAGNNNNNNTAC 2 cut(s) 489, 521
BauI CACGAG 1 cut(s) 1191
BbsI GAAGAC 4 cut(s) 3, 33, 138, 2126
BbvCI CCTCAGC 1 cut(s) 4944
BceAI ACGGC 3 cut(s) 2397, 3779, 5900
BcgI CGANNNNNNTGC 4 cut(s) 1276, 1310, 4359, 4393
BciVI GTATCC 2 cut(s) 3133, 5238
BclI TGATCA 3 cut(s) 466, 3451, 4392
BfmI CTRYAG 6 cut(s) 651, 876, 2217, 2322, 4203, 5697
BfoI RGCGCY 2 cut(s) 867, 5811
BfrI CTTAAG 1 cut(s) 3584
BfuAI ACCTGC 1 cut(s) 5972
BfuI GTATCC 2 cut(s) 3133, 5238
BglI GCCNNNNNGGC 1 cut(s) 416
BglII AGATCT 4 cut(s) 2696, 2939, 5183, 5660
BlnI CCTAGG 1 cut(s) 1792
BmcAI AGTACT 3 cut(s) 351, 2820, 5015
Bme18I GGWCC 3 cut(s) 5173, 6107, 6375
BmeT110I CYCGRG 2 cut(s) 1223, 2088
BmgBI CACGTC 3 cut(s) 39, 179, 4018
BmgT120I GGNCC 6 cut(s) 709, 1816, 2606, 5173, 6107, 6375
BmiI GGNNCC 7 cut(s) 33, 277, 1663, 3034, 3502, 3568, 5028
BmrI ACTGGG 6 cut(s) 679, 1061, 2318, 3283, 3569, 6260
BmuI ACTGGG 6 cut(s) 679, 1061, 2318, 3283, 3569, 6260
BoxI GACNNNNGTC 1 cut(s) 339
BpiI GAAGAC 4 cut(s) 3, 33, 138, 2126
BplI GAGNNNNNCTC 2 cut(s) 3074, 3106
BpmI CTGGAG 6 cut(s) 634, 780, 950, 3666, 4055, 4920
Bpu10I CCTNAGC 3 cut(s) 4944, 5757, 5835
BpuEI CTTGAG 9 cut(s) 647, 1932, 2843, 3101, 3122, 3562, 4442, 5743, 6377
BsaAI YACGTR 2 cut(s) 3512, 6171
BsaBI GATNNNNATC 2 cut(s) 2701, 4381
BsaI GGTCTC 5 cut(s) 662, 1578, 1914, 3538, 5349
BsaWI WCCGGW 1 cut(s) 449
BsaXI ACNNNNNCTCC 4 cut(s) 2524, 2554, 6189, 6219
Bsc4I CCNNNNNNNGG 8 cut(s) 201, 2596, 2956, 3172, 3295, 5986, 6058, 6194
Bse118I RCCGGY 1 cut(s) 2996
Bse3DI GCAATG 8 cut(s) 1430, 2407, 4592, 5259, 5474, 5594, 6004, 6109
Bse8I GATNNNNATC 2 cut(s) 2701, 4381
BseAI TCCGGA 1 cut(s) 449
BseJI GATNNNNATC 2 cut(s) 2701, 4381
BseLI CCNNNNNNNGG 8 cut(s) 201, 2596, 2956, 3172, 3295, 5986, 6058, 6194
BseMI GCAATG 8 cut(s) 1430, 2407, 4592, 5259, 5474, 5594, 6004, 6109
BseRI GAGGAG 3 cut(s) 1536, 2884, 6102
BseSI GKGCMC 4 cut(s) 2353, 3166, 3571, 6189
BseX3I CGGCCG 1 cut(s) 678
BseYI CCCAGC 3 cut(s) 1480, 4211, 5582
BsgI GTGCAG 5 cut(s) 1574, 1677, 2211, 2805, 4077
Bsh1236I CGCG 1 cut(s) 6349
Bsh1285I CGRYCG 1 cut(s) 681
BshNI GGYRCC 2 cut(s) 275, 3566
BsiEI CGRYCG 1 cut(s) 681
BsiHKCI CYCGRG 2 cut(s) 1223, 2088
BsiSI CCGG 2 cut(s) 450, 2997
BslFI GGGAC 4 cut(s) 685, 2957, 4593, 6210
BslI CCNNNNNNNGG 8 cut(s) 201, 2596, 2956, 3172, 3295, 5986, 6058, 6194
BsmBI CGTCTC 2 cut(s) 719, 6150
BsmFI GGGAC 4 cut(s) 685, 2957, 4593, 6210
BsmI GAATGC 7 cut(s) 973, 2344, 2945, 5056, 5535, 5790, 6390
Bso31I GGTCTC 5 cut(s) 662, 1578, 1914, 3538, 5349
BsoBI CYCGRG 2 cut(s) 1223, 2088
Bsp13I TCCGGA 1 cut(s) 449
Bsp1407I TGTACA 1 cut(s) 1330
Bsp19I CCATGG 2 cut(s) 1230, 2543
BspEI TCCGGA 1 cut(s) 449
BspFNI CGCG 1 cut(s) 6349
BspHI TCATGA 2 cut(s) 5107, 5490
BspLI GGNNCC 7 cut(s) 33, 277, 1663, 3034, 3502, 3568, 5028
BspMAI CTGCAG 2 cut(s) 2221, 5701
BspMI ACCTGC 1 cut(s) 5972
BspQI GCTCTTC 1 cut(s) 3849
BspT107I GGYRCC 2 cut(s) 275, 3566
BspTI CTTAAG 1 cut(s) 3584
BspTNI GGTCTC 5 cut(s) 662, 1578, 1914, 3538, 5349
BsrBI CCGCTC 3 cut(s) 273, 1598, 3526
BsrDI GCAATG 8 cut(s) 1430, 2407, 4592, 5259, 5474, 5594, 6004, 6109
BsrFI RCCGGY 1 cut(s) 2996
BsrGI TGTACA 1 cut(s) 1330
BssAI RCCGGY 1 cut(s) 2996
BssNAI GTATAC 1 cut(s) 6298
BssSI CACGAG 1 cut(s) 1191
BssT1I CCWWGG 7 cut(s) 930, 1230, 1792, 2543, 2707, 4071, 6123
Bst1107I GTATAC 1 cut(s) 6298
Bst2BI CACGAG 1 cut(s) 1191
Bst6I CTCTTC 3 cut(s) 3188, 3849, 5709
BstAFI CTTAAG 1 cut(s) 3584
BstAPI GCANNNNNTGC 5 cut(s) 110, 2225, 2348, 5411, 5471
BstAUI TGTACA 1 cut(s) 1330
BstBAI YACGTR 2 cut(s) 3512, 6171
BstDSI CCRYGG 3 cut(s) 1230, 2543, 5916
BstENI CCTNNNNNAGG 1 cut(s) 6056
BstFNI CGCG 1 cut(s) 6349
BstH2I RGCGCY 2 cut(s) 867, 5811
BstHHI GCGC 4 cut(s) 866, 3225, 3244, 5810
BstMCI CGRYCG 1 cut(s) 681
BstNSI RCATGY 7 cut(s) 137, 145, 943, 1647, 3922, 5409, 6403
BstPAI GACNNNNGTC 1 cut(s) 339
BstSFI CTRYAG 6 cut(s) 651, 876, 2217, 2322, 4203, 5697
BstSLI GKGCMC 4 cut(s) 2353, 3166, 3571, 6189
BstUI CGCG 1 cut(s) 6349
BstV2I GAAGAC 4 cut(s) 3, 33, 138, 2126
BstXI CCANNNNNNTGG 2 cut(s) 516, 4718
BstZ17I GTATAC 1 cut(s) 6298
BstZI CGGCCG 1 cut(s) 678
BsuI GTATCC 2 cut(s) 3133, 5238
BtgI CCRYGG 3 cut(s) 1230, 2543, 5916
BtrI CACGTC 3 cut(s) 39, 179, 4018
BtsI GCAGTG 4 cut(s) 2813, 3319, 4272, 5830
BveI ACCTGC 1 cut(s) 5972
CaiI CAGNNNCTG 5 cut(s) 1073, 2225, 3356, 4892, 5296
CciI TCATGA 2 cut(s) 5107, 5490
CciNI GCGGCCGC 1 cut(s) 678
CfoI GCGC 4 cut(s) 866, 3225, 3244, 5810
Cfr10I RCCGGY 1 cut(s) 2996
Cfr13I GGNCC 6 cut(s) 709, 1816, 2606, 5173, 6107, 6375
CseI GACGC 4 cut(s) 329, 687, 1950, 3791
CsiI ACCWGGT 4 cut(s) 1497, 3562, 4251, 6013
DraI TTTAAA 3 cut(s) 2841, 4048, 4080
EaeI YGGCCR 6 cut(s) 678, 2505, 2598, 2994, 3297, 4877
EagI CGGCCG 1 cut(s) 678
Eam1104I CTCTTC 3 cut(s) 3188, 3849, 5709
EarI CTCTTC 3 cut(s) 3188, 3849, 5709
Ecl136II GAGCTC 6 cut(s) 1088, 2093, 3650, 3842, 4915, 5653
EclXI CGGCCG 1 cut(s) 678
Eco130I CCWWGG 7 cut(s) 930, 1230, 1792, 2543, 2707, 4071, 6123
Eco24I GRGCYC 9 cut(s) 1090, 2095, 3652, 3686, 3844, 4917, 5379, 5655, 5731
Eco31I GGTCTC 5 cut(s) 662, 1578, 1914, 3538, 5349
Eco32I GATATC 2 cut(s) 646, 1078
Eco47I GGWCC 3 cut(s) 5173, 6107, 6375
Eco52I CGGCCG 1 cut(s) 678
Eco53kI GAGCTC 6 cut(s) 1088, 2093, 3650, 3842, 4915, 5653
Eco88I CYCGRG 2 cut(s) 1223, 2088
EcoICRI GAGCTC 6 cut(s) 1088, 2093, 3650, 3842, 4915, 5653
EcoNI CCTNNNNNAGG 1 cut(s) 6056
EcoO109I RGGNCCY 1 cut(s) 2606
EcoRI GAATTC 1 cut(s) 1413
EcoRV GATATC 2 cut(s) 646, 1078
EcoT14I CCWWGG 7 cut(s) 930, 1230, 1792, 2543, 2707, 4071, 6123
EcoT22I ATGCAT 3 cut(s) 2344, 2452, 4960
EcoT38I GRGCYC 9 cut(s) 1090, 2095, 3652, 3686, 3844, 4917, 5379, 5655, 5731
ErhI CCWWGG 7 cut(s) 930, 1230, 1792, 2543, 2707, 4071, 6123
Esp3I CGTCTC 2 cut(s) 719, 6150
FalI AAGNNNNNCTT 2 cut(s) 762, 794
FaqI GGGAC 4 cut(s) 685, 2957, 4593, 6210
FauI CCCGC 1 cut(s) 3064
FauNDI CATATG 1 cut(s) 5736
FbaI TGATCA 3 cut(s) 466, 3451, 4392
FblI GTMKAC 1 cut(s) 6297
FriOI GRGCYC 9 cut(s) 1090, 2095, 3652, 3686, 3844, 4917, 5379, 5655, 5731
FspAI RTGCGCAY 1 cut(s) 3224
FspI TGCGCA 1 cut(s) 3224
GlaI GCGC 4 cut(s) 865, 3224, 3243, 5809
GsaI CCCAGC 3 cut(s) 1484, 4215, 5586
GsuI CTGGAG 6 cut(s) 634, 780, 950, 3666, 4055, 4920
HaeII RGCGCY 2 cut(s) 867, 5811
HapII CCGG 2 cut(s) 450, 2997
HgaI GACGC 4 cut(s) 329, 687, 1950, 3791
HhaI GCGC 4 cut(s) 866, 3225, 3244, 5810
Hin6I GCGC 4 cut(s) 864, 3223, 3242, 5808
HinP1I GCGC 4 cut(s) 864, 3223, 3242, 5808
HincII GTYRAC 6 cut(s) 402, 858, 3916, 4011, 5883, 6246
HindII GTYRAC 6 cut(s) 402, 858, 3916, 4011, 5883, 6246
HindIII AAGCTT 8 cut(s) 771, 1360, 1739, 2045, 2572, 2925, 5223, 5422
HpaII CCGG 2 cut(s) 450, 2997
HphI GGTGA 9 cut(s) 141, 959, 1139, 1808, 2396, 2690, 3049, 3277, 5737
HpyCH4IV ACGT 6 cut(s) 38, 178, 3511, 4017, 6143, 6170
HpySE526I ACGT 6 cut(s) 38, 178, 3511, 4017, 6143, 6170
HspAI GCGC 4 cut(s) 864, 3223, 3242, 5808
Kpn2I TCCGGA 1 cut(s) 449
Ksp22I TGATCA 3 cut(s) 466, 3451, 4392
LguI GCTCTTC 1 cut(s) 3849
MabI ACCWGGT 4 cut(s) 1497, 3562, 4251, 6013
MaeII ACGT 6 cut(s) 38, 178, 3511, 4017, 6143, 6170
MbiI CCGCTC 3 cut(s) 273, 1598, 3526
MfeI CAATTG 5 cut(s) 1475, 2199, 2360, 4143, 4500
MlsI TGGCCA 3 cut(s) 2507, 3299, 4879
MluNI TGGCCA 3 cut(s) 2507, 3299, 4879
MlyI GAGTC 4 cut(s) 407, 1346, 1400, 1793
Mox20I TGGCCA 3 cut(s) 2507, 3299, 4879
Mph1103I ATGCAT 3 cut(s) 2344, 2452, 4960
MroI TCCGGA 1 cut(s) 449
MroXI GAANNNNTTC 5 cut(s) 1413, 3330, 4473, 4758, 5934
MscI TGGCCA 3 cut(s) 2507, 3299, 4879
MslI CAYNNNNRTG 5 cut(s) 1103, 1678, 3062, 4099, 5193
Msp20I TGGCCA 3 cut(s) 2507, 3299, 4879
MspA1I CMGCKG 6 cut(s) 2592, 2984, 4064, 4202, 4973, 5672
MspCI CTTAAG 1 cut(s) 3584
MspI CCGG 2 cut(s) 450, 2997
MunI CAATTG 5 cut(s) 1475, 2199, 2360, 4143, 4500
Mva1269I GAATGC 7 cut(s) 973, 2344, 2945, 5056, 5535, 5790, 6390
MvnI CGCG 1 cut(s) 6349
NcoI CCATGG 2 cut(s) 1230, 2543
NdeI CATATG 1 cut(s) 5736
NlaIV GGNNCC 7 cut(s) 33, 277, 1663, 3034, 3502, 3568, 5028
NmuCI GTSAC 4 cut(s) 568, 1622, 1796, 6259
NotI GCGGCCGC 1 cut(s) 678
NsbI TGCGCA 1 cut(s) 3224
NsiI ATGCAT 3 cut(s) 2344, 2452, 4960
NspI RCATGY 7 cut(s) 137, 145, 943, 1647, 3922, 5409, 6403
PaeI GCATGC 1 cut(s) 943
PaeR7I CTCGAG 2 cut(s) 1223, 2088
PagI TCATGA 2 cut(s) 5107, 5490
PciI ACATGT 1 cut(s) 6399
PciSI GCTCTTC 1 cut(s) 3849
PctI GAATGC 7 cut(s) 973, 2344, 2945, 5056, 5535, 5790, 6390
PdmI GAANNNNTTC 5 cut(s) 1413, 3330, 4473, 4758, 5934
PflMI CCANNNNNTGG 1 cut(s) 3295
PfoI TCCNGGA 1 cut(s) 966
PleI GAGTC 4 cut(s) 406, 1345, 1399, 1793
PpsI GAGTC 4 cut(s) 406, 1345, 1399, 1793
Ppu21I YACGTR 2 cut(s) 3512, 6171
PscI ACATGT 1 cut(s) 6399
PshAI GACNNNNGTC 1 cut(s) 339
PshBI ATTAAT 2 cut(s) 1770, 5709
PsiI TTATAA 1 cut(s) 1310
Psp124BI GAGCTC 6 cut(s) 1090, 2095, 3652, 3844, 4917, 5655
PspFI CCCAGC 3 cut(s) 1480, 4211, 5582
PspN4I GGNNCC 7 cut(s) 33, 277, 1663, 3034, 3502, 3568, 5028
PspPI GGNCC 6 cut(s) 709, 1816, 2606, 5173, 6107, 6375
PspXI VCTCGAGB 2 cut(s) 1223, 2088
PsrI GAACNNNNNNTAC 2 cut(s) 3016, 3048
PstI CTGCAG 2 cut(s) 2221, 5701
PstNI CAGNNNCTG 5 cut(s) 1073, 2225, 3356, 4892, 5296
PvuII CAGCTG 5 cut(s) 2984, 4064, 4202, 4973, 5672
RseI CAYNNNNRTG 5 cut(s) 1103, 1678, 3062, 4099, 5193
SacI GAGCTC 6 cut(s) 1090, 2095, 3652, 3844, 4917, 5655
SapI GCTCTTC 1 cut(s) 3849
Sau96I GGNCC 6 cut(s) 709, 1816, 2606, 5173, 6107, 6375
ScaI AGTACT 3 cut(s) 351, 2820, 5015
SchI GAGTC 4 cut(s) 407, 1346, 1400, 1793
SexAI ACCWGGT 4 cut(s) 1497, 3562, 4251, 6013
SfcI CTRYAG 6 cut(s) 651, 876, 2217, 2322, 4203, 5697
Sfr274I CTCGAG 2 cut(s) 1223, 2088
SinI GGWCC 3 cut(s) 5173, 6107, 6375
SlaI CTCGAG 2 cut(s) 1223, 2088
SmiMI CAYNNNNRTG 5 cut(s) 1103, 1678, 3062, 4099, 5193
SphI GCATGC 1 cut(s) 943
SspI AATATT 6 cut(s) 262, 486, 997, 3729, 4612, 4824
SstI GAGCTC 6 cut(s) 1090, 2095, 3652, 3844, 4917, 5655
StyI CCWWGG 7 cut(s) 930, 1230, 1792, 2543, 2707, 4071, 6123
TaiI ACGT 6 cut(s) 41, 181, 3514, 4020, 6146, 6173
TaqI TCGA 7 cut(s) 5, 1224, 2089, 3695, 5645, 6115, 6363
TaqII GACCGA 2 cut(s) 45, 4383
TatI WGTACW 6 cut(s) 349, 1330, 1843, 2818, 4108, 5013
TauI GCSGC 6 cut(s) 276, 305, 425, 680, 683, 1598
TseFI GTSAC 4 cut(s) 568, 1622, 1796, 6259
Tsp45I GTSAC 4 cut(s) 568, 1622, 1796, 6259
Van91I CCANNNNNTGG 1 cut(s) 3295
Vha464I CTTAAG 1 cut(s) 3584
VpaK11BI GGWCC 3 cut(s) 5173, 6107, 6375
VspI ATTAAT 2 cut(s) 1770, 5709
XagI CCTNNNNNAGG 1 cut(s) 6056
XapI RAATTY 8 cut(s) 1413, 1612, 2527, 2637, 2881, 3346, 5482, 5606
XbaI TCTAGA 1 cut(s) 2699
XceI RCATGY 7 cut(s) 137, 145, 943, 1647, 3922, 5409, 6403
XcmI CCANNNNNNNNNTGG 1 cut(s) 2515
XhoI CTCGAG 2 cut(s) 1223, 2088
XmaJI CCTAGG 1 cut(s) 1792
XmiI GTMKAC 1 cut(s) 6297
XmnI GAANNNNTTC 5 cut(s) 1413, 3330, 4473, 4758, 5934
ZrmI AGTACT 3 cut(s) 351, 2820, 5015
Zsp2I ATGCAT 3 cut(s) 2344, 2452, 4960
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.