Rmu_sc0004708.1_g000010

Protein kinase C conserved region 2 (CalB)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004708.1
OG0009147
Physical Location & Seq
Forward (+)
39485 .. 49147
9663 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0004708.1_g000010.1.cds

Sequence Viewer

Length: 6633 bp
atggaaaggaaacgaaaagactgggactcgctgctctctgtgtcactctgtcagccctccattttcactactctctctgatatctccgccgctgctccactctcattccggtcattctccggcactgaattccctccatttcaagagaggtggatttgttttggaccgggaaggaagtttgcattctgtggccaaggagaaattcaaaagaggcgtatactgcaaatgtcaaagtcttcctcccctgaactacgggaacccgtgtcttcttctacttctcggtctagggatttagatgaaacaacaatggatgatgaagaaggtacaatggcaagtgttgctgaatttgttgagaaactacatgccagcatgtcttcaccgcatgaaaaagaacttattacagcacgtctgctaggtattgccaaagcaaggaaggatgcaagaacaattattggttcacattcccaggctatgccattgttcataaatattctgaggagtggcacccctgttgcaaaggttaatgttgcggcaactctgagtgtcctgtgcaaagatgaagacttgcgtcttaaagtacttctaggggggtgcatccccccgttactctcacttttgaagtctgaatcaactgaggccaggaaggctgcagccgaagctatatatgaagtttcctctggcgggctttcagatgatcacgttggcataaaaatatttattacagaaggtgttgtaccaaacttgtggaatcagctcaatccaaacagcaaccaggacaaagtggtagagggatttgtgacgggggctttaagaaatctctgtggtgacaaggatggctactggagagcaacacttgaggctggtggagtagatatcactgtgggtctcctgtattctgacaatgtggctgcccaatccaatgctgcgtcccttttggcccgtctcatgttggctttcagtgatagcattcccaaagtaatagattctggagctgtcaaagctttgcttcggctcgtgggtcaagaaaataatgtttctgttcgtgctagtgctgctgatgctctggaagctctttcttcaaagtcaactggtgctaagaaagctattgttgatgcaaatggtcttccagttctgattggagctattgttgctccatctaaggagtgcatgcaaggtgagtgcgggcaggctctccaggagcattcaacaagggctttagcaaatatttgtggtggggtgtctgctttgatactttatcttggagagctttcacagtcagctcgcctagctgccccggttgctgatattattggagctcttgcatacactttaatggtctttgagcaaaaatctggtgatggtaaggaatcttttaaggtaacaaagatagaggatattcttgtgatgctgctaaagcctcgtgataataagcttgttcaagagcgtgtccttgaggccatggctagtctatatggcaacattcatctctcaaaatggctcaatcatgcagaagcgaagaaggtgcttattggacttataacaatggctgctgctgatgtacaagagtctctgatactctctttgacaagcttatgctgtgatggtgttggaatctgggagtccattggcgagagagaaggaattcagctacttatttcattgcttggcttatccagtgagcagcatcaagaatatgctgttcagttgctagggatcttaactgaccaggttgatgacagcaagtgggctattactgctgctggtgggattcctcctctagtgcagttgttagagacgggatcgcaaaaggcgaaggaggacgctgctcatgtgttgtggaacttgtgctgtcacagtgaggatattcgtgcatgtgttgaaagtgcaggagccattccagcatttttatggcttctaaaaagtggtggatcaagggggcaagaagcatcagctatggcacttacaaagcttatccgaacagccgattcggctaccattaatcagttgttagctttgctcttaggtgactctccatgctcaaaggcccatactatcagggtgttgggtcatgtactcacaatggcatcacacaaggatcttgtgcataagggttcagcagctaataaagggttgaggtctctcgttcaggttctcaactcatcaaatgaagaaactcaagagtatgccgcgtctgtcttagctgatctattcagcatgagacaagatatttgtgacactcttgcaactgatgagatcgtgcatccttgcatgaagcttttgaccagcagcacacaagctgttgcaacacagtcggctcgagctttgggtgctctttcccgtcctatgaagaccaaaacaataagcaagatgtcttatattgcagaaggggatgttaaacccctcatcaaactagcaaaaacttcttcaattgatgctgctcaaactgcagttgctgccctcgccaatcttctctctgatccccagattgctgcagaagccctggcagaagatgttgtttcagctttgataagagtacttggagatggaactacagaaggtaaaaagaatgcatcccgtgcccttcatcaattgttgaagcattttccagtaggtgatgtgctcacaggaaatgctcattgccattatgctattcttgctgttgttgattccttaaatgcattggatatggatgagaccgatgctgctgatgctttagaagtagtagcacttttggccagaaccaaacagggtgtgaacttcactgaccctccatggtctgtatttgctgaagttgaatcgagcttagaacctcttgtccgctgtctggccgaagggtctcctttgcttcaggataaggcaatagaagttttgtctaggctttgcggggagcaaccagttgtgcttggtgatctattgattgcaagatctggatcccttggttcactagctaacagaataatgaactcatctagtttagaagtcagagttggaggagctgcattactcatttgtgcagcaaaagagcacaaagagcagtggatggaagtacttgaggtgtcaggatatttaaaaccattaatgtatgctttagtggatatgatgaagcaaaattgtagctgctcctctttagaaattgaagtccgaacttctaaagcttttatggaaagaactgcattccacgaaggggatgagtttgatgtccctgatccagccattgtcttggggggtactgttgctttgtggttgctatgtataattggttcctgtaatgcaaagagcaaactcaccattatggaagccggaggtctcgaggctctctctgacaagcttgagagccacacttccaatccacaggcagattatgaggatactgaaggtatatggattagtgccctgcttctggctattctcttccaagatgccaatgtcgtttcatctcctacaacaatgcggatcatagcttctcttgcgcttctgctgagatcagatgaggtgattgataggttctttgctgcccagtcaatggccagtcttgtttgcattggtaataagggaataattcttgccattgcaaattcaggtgctgttgctggattgataaccctcattggttatgtagaatcggatatgcctaaccttgttactttatcagaagagttttctctggtgcgaaaccctgatcaagttgttttggaatacctttttgattttgaagatgtaagagttggatccacagcacgcaaatctatacctctcttggtggatctcttgagaccaatgccagatagacctggtgcccctccagttgctcttaaactcttgacctgtattgctgatggaagtgatacaaacaaattagtcatggctgaagctggagctctggacgctctgacaaagtacctgtctttgagcccacaagattcgactgaagctgcaatatctgatttattcagaatattgtttagccatcctgatctgattcgctatgaagcatcagctagttcgttgaatcagctcatagctgttctgcgtctggggtcaagaaatgctagatacagtgctgcaagagctcttcacgaactatttgatgctcagaacatcagaaattctgatttagcgaggcaatctgttcagccattggttgacatgcttaatgctgcatcagaaaatgaacaagaggctgctcttgttgcggtaattaagctgacttccggaaattcttctaccgcagctttgttgactgatgtggagggaaatccactggagagtttatttaaaatattgtcttctgcttcatccttggatttgaagagacttgctgcacaactatgttgtgtactgtttgatagtaccaaagtcagaggaaatccaattgcctctgaatgtatagagcctcttatttccttgatgctttctggtattaatgcagctgtagaagctggggtttgtgcctttgaaaaattgttggatgatgaacaccaggtagagcttgcagtgaactataatgttgtcgatcttcttgttggattagtttctggtacgaacagtcagctcattgaggctagcatctgctctctcataaagttggggaaagaccggacccaatgcaaatcggatatgatcaatgctggcattattgataactgtcttgagctactccctgttgcagccagttcattatgctcttccattgctgaattatttcgcattttgacaaatagtgatgcaattgctagaagttcagctgctgcaacaattgttgaacctcttttcctagttttacttcgtccagatttcagtatgtggggacagcacagtgcattgcaagcactagtaaatattttggagaagccacaaagccttgccactttaaaacttacgcctagccaagtcattgagcctctgatttcatttcttgaatccccatctctagccatccagcagcttggtacagaattgctatctcatcttcttgcacaagaacattttcagcaagatattacaacaaaaaatgctgttgttcctcttgtgcaacttgcgggaattggaatactgaacttacagcaaacagcgataaaggcattggaaaaaatatccacaagatggccaaaggcagttgctgatgctggaggtatttttgagcttggaaaggttattatacaagatgatcctcagccacctcatgcattgtgggaatcagctgctttagttctctcaaatgttttgtgcttcaatgctgagtactactttaaagttcctgtggtggttcttgttaaaatgttgcattcaacagtggatagcactattacggtggctctcaatgcactacttgttcatgaaaggagtgataagctaagtgctgaacagatgactgaaaatggtgcaatagatgccttgttggaccttttaacatctcatcagtgtgaagaagcatctggaaggttacttgaagcgttattcaacaatgtcagcatacgagcaatgaaggtttccaagtatgcaatagcacctttatcacagtatctgttggattcacaaaccaaatcacaatctggaaaacttcttgtagctctagctcttggagacctttcccaacacgaagggcttgctagagccagtgattctgtttctgcatgtcgggcattaataagcttgcttgaagatcagcccacagaagatatgaaaatggtggcaatttgtgcattgcaaaattttgtcatgaacagccgaacgaatcggcgtgctgtcgcagaagcagggggaatactggttattcaagaactactactgtctctcaatgcagaagttgctgggcaagcagcattgcttatcaaatttttgttttctaatcacacacttcaagaatatgtgtcaaacgagctcatcagatctttgacagccgcattagagagagagttgtggtccacagcaactattaatgaagaggttttgagagccctacatatgatattcaccaactttcctaagctccatatttccgaggcagccactctctgcattcccaatctgattggagcccttaaatctggcagtgaggcagctcaggatgtcgtgttggacaccctttgcttgttgagacattcttggtcaaccatgccaatagatattgcaaagtctcaatccgtggttgctgctgaagccattcccatcttacaaatgcttatgaaaacctgcccaccaagtttccatgagagagcagacagcctgttgcattgcttaccaggttgtttgactgttacaatcaagcgtggaaacaaccttaaacaggctatgggaggcacaaatgctttttgccgattgacaataggcaacggtcctcctcgacaaaccaaggtagtgagccacagtacttctcccgaatggaaagaagggtttacgtgggcatttgatgtgcccccgaagggacaaaaactccacatcatctgcaaaagcaaaaatacctttggaaagtcaacactggggagagtgactatccagatcgataaagttgtgtcagaaggagtatacagtggattattcagcctaaaccatgacaacagcaaagatggatcttcacgtacacttgaaatcgagattatctggtccaacagaatgcccgatgatgaaatgtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000271 GO:0000902 GO:0002682 GO:0002683 GO:0003674 GO:0003824 GO:0004842 GO:0005102 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005856 GO:0005874 GO:0005881 GO:0005886 GO:0005938 GO:0005975 GO:0005976 GO:0006073 GO:0006464 GO:0006807 GO:0006950 GO:0006952 GO:0008104 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009250 GO:0009605 GO:0009607 GO:0009617 GO:0009653 GO:0009664 GO:0009826 GO:0009832 GO:0009987 GO:0010330 GO:0010383 GO:0010941 GO:0015630 GO:0016020 GO:0016043 GO:0016049 GO:0016051 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0030243 GO:0030244 GO:0030863 GO:0030981 GO:0032446 GO:0032502 GO:0032989 GO:0032991 GO:0033036 GO:0033365 GO:0033612 GO:0033692 GO:0034613 GO:0034637 GO:0034645 GO:0036211 GO:0040007 GO:0042546 GO:0042742 GO:0042981 GO:0043066 GO:0043067 GO:0043069 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043412 GO:0044036 GO:0044038 GO:0044042 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044262 GO:0044264 GO:0044267 GO:0044380 GO:0044422 GO:0044424 GO:0044430 GO:0044444 GO:0044446 GO:0044448 GO:0044464 GO:0045229 GO:0048519 GO:0048523 GO:0048580 GO:0048583 GO:0048585 GO:0048589 GO:0048856 GO:0048869 GO:0050776 GO:0050777 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051179 GO:0051239 GO:0051273 GO:0051274 GO:0051641 GO:0051704 GO:0051707 GO:0052324 GO:0052541 GO:0055028 GO:0060548 GO:0060560 GO:0065007 GO:0070589 GO:0070592 GO:0070647 GO:0070696 GO:0070727 GO:0071554 GO:0071555 GO:0071669 GO:0071704 GO:0071840 GO:0071944 GO:0072697 GO:0072698 GO:0072699 GO:0098542 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0099568 GO:0140096 GO:1901564 GO:1901576 GO:1902494 GO:1990234 GO:1990778 GO:2000026 GO:2000028 GO:2000241
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Arm C2

Protein Analysis

2210

Amino Acids

237.52

Weight (kDa)

5.29

Isoelectric Point (pI)

39.35

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1538
Acc36I ACCTGC 1 cut(s) 6200
AccB1I GGYRCC 2 cut(s) 503, 3794
AccB7I CCANNNNNTGG 2 cut(s) 3523, 5103
AccI GTMKAC 2 cut(s) 217, 6525
AccII CGCG 1 cut(s) 2189
AccIII TCCGGA 1 cut(s) 4211
AcoI YGGCCR 5 cut(s) 190, 2733, 2826, 3525, 5105
AcsI RAATTY 9 cut(s) 128, 201, 344, 1641, 3574, 4105, 4216, 5710, 5834
AcuI CTGAAG 6 cut(s) 2808, 2831, 3393, 3888, 3948, 6177
AfiI CCNNNNNNNGG 9 cut(s) 429, 681, 2824, 3184, 3400, 3523, 5103, 6286, 6422
AhlI ACTAGT 1 cut(s) 4831
AjiI CACGTC 1 cut(s) 407
AjnI CCWGG 9 cut(s) 465, 638, 771, 1194, 1725, 2499, 3790, 4479, 6241
AjuI GAANNNNNNNTTGG 4 cut(s) 186, 218, 2970, 3002
AloI GAACNNNNNNTCC 4 cut(s) 2799, 2831, 4755, 4787
Alw21I GWGCWC 7 cut(s) 1318, 2332, 2622, 3027, 3880, 4072, 5883
AlwNI CAGNNNCTG 5 cut(s) 2453, 3584, 4748, 5120, 5524
Ama87I CYCGRG 2 cut(s) 2316, 3308
Aor13HI TCCGGA 1 cut(s) 4211
AoxI GGCC 9 cut(s) 190, 636, 936, 1455, 2043, 2733, 2826, 3525, 5105
ApoI RAATTY 9 cut(s) 128, 201, 344, 1641, 3574, 4105, 4216, 5710, 5834
ArsI GACNNNNNNTTYG 4 cut(s) 2211, 2243, 3887, 3919
AseI ATTAAT 5 cut(s) 1998, 3077, 4422, 5645, 5937
Asp700I GAANNNNTTC 5 cut(s) 1641, 4219, 4701, 4986, 6162
AspLEI GCGC 1 cut(s) 3472
AspS9I GGNCC 8 cut(s) 164, 937, 2044, 4599, 5401, 5922, 6335, 6603
AsuC2I CCSGG 2 cut(s) 168, 1295
AsuNHI GCTAGC 1 cut(s) 4562
AvaI CYCGRG 2 cut(s) 2316, 3308
AvaII GGWCC 6 cut(s) 164, 4599, 5401, 5922, 6335, 6603
BaeGI GKGCMC 4 cut(s) 2581, 3394, 3799, 6417
BalI TGGCCA 4 cut(s) 192, 2735, 3527, 5107
BamHI GGATCC 2 cut(s) 2930, 3728
BanI GGYRCC 2 cut(s) 503, 3794
BanII GRGCYC 7 cut(s) 1318, 3880, 3914, 4072, 5883, 5959, 6040
BarI GAAGNNNNNNTAC 2 cut(s) 717, 749
BauI CACGAG 2 cut(s) 1013, 1419
BbsI GAAGAC 7 cut(s) 228, 258, 366, 567, 1115, 2354, 4278
Bbv12I GWGCWC 7 cut(s) 1318, 2332, 2622, 3027, 3880, 4072, 5883
BbvCI CCTCAGC 1 cut(s) 5172
BcgI CGANNNNNNTGC 6 cut(s) 1504, 1538, 2292, 2326, 5588, 5622
BciT130I CCWGG 9 cut(s) 467, 640, 773, 1196, 1727, 2501, 3792, 4481, 6243
BciVI GTATCC 1 cut(s) 3361
BclI TGATCA 3 cut(s) 694, 3679, 4620
BcnI CCSGG 2 cut(s) 168, 1295
BcuI ACTAGT 1 cut(s) 4831
BfmI CTRYAG 5 cut(s) 648, 2445, 2490, 2550, 4431
BfuAI ACCTGC 1 cut(s) 6200
BfuI GTATCC 1 cut(s) 3361
BglI GCCNNNNNGGC 2 cut(s) 644, 1988
BglII AGATCT 2 cut(s) 2924, 5888
BmcAI AGTACT 5 cut(s) 579, 2535, 3048, 5243, 6370
Bme18I GGWCC 6 cut(s) 164, 4599, 5401, 5922, 6335, 6603
BmeT110I CYCGRG 2 cut(s) 2316, 3308
BmgBI CACGTC 1 cut(s) 407
BmgT120I GGNCC 8 cut(s) 164, 937, 2044, 4599, 5401, 5922, 6335, 6603
BmiI GGNNCC 9 cut(s) 258, 505, 1891, 2932, 3262, 3730, 3796, 4601, 6037
BmrI ACTGGG 3 cut(s) 31, 3511, 6488
BmtI GCTAGC 1 cut(s) 4566
BmuI ACTGGG 3 cut(s) 31, 3511, 6488
BoxI GACNNNNGTC 1 cut(s) 567
BpiI GAAGAC 7 cut(s) 228, 258, 366, 567, 1115, 2354, 4278
BplI GAGNNNNNCTC 2 cut(s) 3302, 3334
BpmI CTGGAG 7 cut(s) 862, 1008, 1178, 3786, 3894, 4283, 5148
Bpu10I CCTNAGC 3 cut(s) 5172, 5985, 6063
BpuEI CTTGAG 7 cut(s) 875, 1472, 2160, 3071, 3350, 3790, 4670
BpuMI CCSGG 2 cut(s) 168, 1295
Bsa29I ATCGAT 1 cut(s) 6501
BsaAI YACGTR 2 cut(s) 6399, 6578
BsaBI GATNNNNATC 1 cut(s) 2929
BsaI GGTCTC 7 cut(s) 890, 2142, 2687, 2841, 3311, 3766, 5577
BsaWI WCCGGW 3 cut(s) 108, 4211, 4596
BsaXI ACNNNNNCTCC 6 cut(s) 1131, 1161, 2752, 2782, 6417, 6447
Bsc4I CCNNNNNNNGG 9 cut(s) 429, 681, 2824, 3184, 3400, 3523, 5103, 6286, 6422
Bse3DI GCAATG 9 cut(s) 1658, 2635, 3567, 4689, 4820, 5487, 5702, 5822, 6232
Bse8I GATNNNNATC 1 cut(s) 2929
BseAI TCCGGA 1 cut(s) 4211
BseBI CCWGG 9 cut(s) 467, 640, 773, 1196, 1727, 2501, 3792, 4481, 6243
BseCI ATCGAT 1 cut(s) 6501
BseJI GATNNNNATC 1 cut(s) 2929
BseLI CCNNNNNNNGG 9 cut(s) 429, 681, 2824, 3184, 3400, 3523, 5103, 6286, 6422
BseMI GCAATG 9 cut(s) 1658, 2635, 3567, 4689, 4820, 5487, 5702, 5822, 6232
BseMII CTCAG 8 cut(s) 485, 530, 624, 3470, 4106, 5186, 5229, 6077
BseRI GAGGAG 5 cut(s) 511, 1764, 3006, 3112, 6330
BseSI GKGCMC 4 cut(s) 2581, 3394, 3799, 6417
BseYI CCCAGC 2 cut(s) 4439, 5810
BsgI GTGCAG 4 cut(s) 1802, 1905, 3033, 4305
Bsh1236I CGCG 1 cut(s) 2189
BshFI GGCC 9 cut(s) 192, 638, 938, 1457, 2045, 2735, 2828, 3527, 5107
BshNI GGYRCC 2 cut(s) 503, 3794
BshVI ATCGAT 1 cut(s) 6501
BsiHKAI GWGCWC 7 cut(s) 1318, 2332, 2622, 3027, 3880, 4072, 5883
BsiHKCI CYCGRG 2 cut(s) 2316, 3308
BsiSI CCGG 7 cut(s) 109, 120, 167, 1295, 3300, 4212, 4597
BslFI GGGAC 5 cut(s) 38, 913, 3185, 4821, 6438
BslI CCNNNNNNNGG 9 cut(s) 429, 681, 2824, 3184, 3400, 3523, 5103, 6286, 6422
BsmBI CGTCTC 2 cut(s) 947, 1787
BsmFI GGGAC 5 cut(s) 38, 913, 3185, 4821, 6438
BsmI GAATGC 8 cut(s) 182, 966, 1201, 2572, 3173, 5284, 6018, 6618
BsnI GGCC 9 cut(s) 192, 638, 938, 1457, 2045, 2735, 2828, 3527, 5107
Bso31I GGTCTC 7 cut(s) 890, 2142, 2687, 2841, 3311, 3766, 5577
BsoBI CYCGRG 2 cut(s) 2316, 3308
Bsp13I TCCGGA 1 cut(s) 4211
Bsp1407I TGTACA 1 cut(s) 1558
Bsp19I CCATGG 2 cut(s) 1458, 2771
BspANI GGCC 9 cut(s) 192, 638, 938, 1457, 2045, 2735, 2828, 3527, 5107
BspCNI CTCAG 8 cut(s) 486, 531, 625, 3471, 4105, 5185, 5230, 6076
BspDI ATCGAT 1 cut(s) 6501
BspEI TCCGGA 1 cut(s) 4211
BspFNI CGCG 1 cut(s) 2189
BspHI TCATGA 2 cut(s) 5335, 5718
BspLI GGNNCC 9 cut(s) 258, 505, 1891, 2932, 3262, 3730, 3796, 4601, 6037
BspMAI CTGCAG 3 cut(s) 652, 2449, 2494
BspMI ACCTGC 1 cut(s) 6200
BspOI GCTAGC 1 cut(s) 4566
BspQI GCTCTTC 2 cut(s) 4077, 4690
BspT107I GGYRCC 2 cut(s) 503, 3794
BspTNI GGTCTC 7 cut(s) 890, 2142, 2687, 2841, 3311, 3766, 5577
BsrDI GCAATG 9 cut(s) 1658, 2635, 3567, 4689, 4820, 5487, 5702, 5822, 6232
BsrGI TGTACA 1 cut(s) 1558
BssNAI GTATAC 2 cut(s) 218, 6526
BssSI CACGAG 2 cut(s) 1013, 1419
BssT1I CCWWGG 6 cut(s) 193, 1458, 2771, 2935, 4299, 6351
Bst1107I GTATAC 2 cut(s) 218, 6526
Bst2BI CACGAG 2 cut(s) 1013, 1419
Bst2UI CCWGG 9 cut(s) 467, 640, 773, 1196, 1727, 2501, 3792, 4481, 6243
Bst6I CTCTTC 6 cut(s) 3416, 3648, 4077, 4304, 4690, 5937
BstAPI GCANNNNNTGC 6 cut(s) 338, 2453, 2576, 5390, 5699, 5807
BstAUI TGTACA 1 cut(s) 1558
BstBAI YACGTR 2 cut(s) 6399, 6578
BstDSI CCRYGG 3 cut(s) 1458, 2771, 6144
BstENI CCTNNNNNAGG 1 cut(s) 6284
BstFNI CGCG 1 cut(s) 2189
BstHHI GCGC 1 cut(s) 3472
BstNI CCWGG 9 cut(s) 467, 640, 773, 1196, 1727, 2501, 3792, 4481, 6243
BstNSI RCATGY 6 cut(s) 365, 373, 1171, 1875, 4150, 5637
BstPAI GACNNNNGTC 1 cut(s) 567
BstSFI CTRYAG 5 cut(s) 648, 2445, 2490, 2550, 4431
BstSLI GKGCMC 4 cut(s) 2581, 3394, 3799, 6417
BstUI CGCG 1 cut(s) 2189
BstV2I GAAGAC 7 cut(s) 228, 258, 366, 567, 1115, 2354, 4278
BstX2I RGATCY 8 cut(s) 1713, 2095, 2924, 2930, 3728, 3763, 5888, 6569
BstXI CCANNNNNNTGG 3 cut(s) 744, 3220, 4946
BstYI RGATCY 8 cut(s) 1713, 2095, 2924, 2930, 3728, 3763, 5888, 6569
BstZ17I GTATAC 2 cut(s) 218, 6526
Bsu15I ATCGAT 1 cut(s) 6501
BsuI GTATCC 1 cut(s) 3361
BsuRI GGCC 9 cut(s) 192, 638, 938, 1457, 2045, 2735, 2828, 3527, 5107
BsuTUI ATCGAT 1 cut(s) 6501
BtgI CCRYGG 3 cut(s) 1458, 2771, 6144
BtrI CACGTC 1 cut(s) 407
BtsI GCAGTG 3 cut(s) 3041, 4500, 6058
BveI ACCTGC 1 cut(s) 6200
CaiI CAGNNNCTG 5 cut(s) 2453, 3584, 4748, 5120, 5524
CciI TCATGA 2 cut(s) 5335, 5718
CfoI GCGC 1 cut(s) 3472
Cfr13I GGNCC 8 cut(s) 164, 937, 2044, 4599, 5401, 5922, 6335, 6603
ClaI ATCGAT 1 cut(s) 6501
CseI GACGC 6 cut(s) 557, 915, 1829, 2178, 3893, 4019
CsiI ACCWGGT 4 cut(s) 1725, 3790, 4479, 6241
CspCI CAANNNNNGTGG 2 cut(s) 131, 166
DraI TTTAAA 4 cut(s) 3069, 4276, 4872, 5251
EaeI YGGCCR 5 cut(s) 190, 2733, 2826, 3525, 5105
Eam1104I CTCTTC 6 cut(s) 3416, 3648, 4077, 4304, 4690, 5937
EarI CTCTTC 6 cut(s) 3416, 3648, 4077, 4304, 4690, 5937
EciI GGCGGA 1 cut(s) 76
Ecl136II GAGCTC 4 cut(s) 1316, 3878, 4070, 5881
Eco130I CCWWGG 6 cut(s) 193, 1458, 2771, 2935, 4299, 6351
Eco24I GRGCYC 7 cut(s) 1318, 3880, 3914, 4072, 5883, 5959, 6040
Eco31I GGTCTC 7 cut(s) 890, 2142, 2687, 2841, 3311, 3766, 5577
Eco32I GATATC 2 cut(s) 82, 874
Eco47I GGWCC 6 cut(s) 164, 4599, 5401, 5922, 6335, 6603
Eco53kI GAGCTC 4 cut(s) 1316, 3878, 4070, 5881
Eco57I CTGAAG 6 cut(s) 2808, 2831, 3393, 3888, 3948, 6177
Eco88I CYCGRG 2 cut(s) 2316, 3308
EcoICRI GAGCTC 4 cut(s) 1316, 3878, 4070, 5881
EcoNI CCTNNNNNAGG 1 cut(s) 6284
EcoRI GAATTC 2 cut(s) 128, 1641
EcoRII CCWGG 9 cut(s) 465, 638, 771, 1194, 1725, 2499, 3790, 4479, 6241
EcoRV GATATC 2 cut(s) 82, 874
EcoT14I CCWWGG 6 cut(s) 193, 1458, 2771, 2935, 4299, 6351
EcoT22I ATGCAT 3 cut(s) 2572, 2680, 5188
EcoT38I GRGCYC 7 cut(s) 1318, 3880, 3914, 4072, 5883, 5959, 6040
ErhI CCWWGG 6 cut(s) 193, 1458, 2771, 2935, 4299, 6351
Esp3I CGTCTC 2 cut(s) 947, 1787
FalI AAGNNNNNCTT 2 cut(s) 990, 1022
FaqI GGGAC 5 cut(s) 38, 913, 3185, 4821, 6438
FauI CCCGC 4 cut(s) 674, 1175, 2876, 5032
FauNDI CATATG 1 cut(s) 5964
FbaI TGATCA 3 cut(s) 694, 3679, 4620
FblI GTMKAC 2 cut(s) 217, 6525
FriOI GRGCYC 7 cut(s) 1318, 3880, 3914, 4072, 5883, 5959, 6040
GlaI GCGC 1 cut(s) 3471
GsaI CCCAGC 2 cut(s) 4443, 5814
GsuI CTGGAG 7 cut(s) 862, 1008, 1178, 3786, 3894, 4283, 5148
HaeIII GGCC 9 cut(s) 192, 638, 938, 1457, 2045, 2735, 2828, 3527, 5107
HapII CCGG 7 cut(s) 109, 120, 167, 1295, 3300, 4212, 4597
HgaI GACGC 6 cut(s) 557, 915, 1829, 2178, 3893, 4019
HhaI GCGC 1 cut(s) 3472
Hin6I GCGC 1 cut(s) 3470
HinP1I GCGC 1 cut(s) 3470
HincII GTYRAC 5 cut(s) 1086, 4144, 4239, 6111, 6474
HindII GTYRAC 5 cut(s) 1086, 4144, 4239, 6111, 6474
HindIII AAGCTT 8 cut(s) 999, 1430, 1588, 1967, 2273, 3153, 3326, 5650
HpaII CCGG 7 cut(s) 109, 120, 167, 1295, 3300, 4212, 4597
HpyCH4IV ACGT 4 cut(s) 406, 699, 6398, 6577
HpySE526I ACGT 4 cut(s) 406, 699, 6398, 6577
HspAI GCGC 1 cut(s) 3470
Kpn2I TCCGGA 1 cut(s) 4211
Ksp22I TGATCA 3 cut(s) 694, 3679, 4620
LguI GCTCTTC 2 cut(s) 4077, 4690
MabI ACCWGGT 4 cut(s) 1725, 3790, 4479, 6241
MaeII ACGT 4 cut(s) 406, 699, 6398, 6577
MfeI CAATTG 5 cut(s) 2427, 2588, 4371, 4728, 4755
MflI RGATCY 8 cut(s) 1713, 2095, 2924, 2930, 3728, 3763, 5888, 6569
MlsI TGGCCA 4 cut(s) 192, 2735, 3527, 5107
MluNI TGGCCA 4 cut(s) 192, 2735, 3527, 5107
MlyI GAGTC 4 cut(s) 20, 1574, 1628, 2021
MmeI TCCRAC 9 cut(s) 1588, 2968, 3706, 4446, 4504, 5379, 5508, 6057, 6630
Mox20I TGGCCA 4 cut(s) 192, 2735, 3527, 5107
Mph1103I ATGCAT 3 cut(s) 2572, 2680, 5188
MroI TCCGGA 1 cut(s) 4211
MroXI GAANNNNTTC 5 cut(s) 1641, 4219, 4701, 4986, 6162
MscI TGGCCA 4 cut(s) 192, 2735, 3527, 5107
MslI CAYNNNNRTG 5 cut(s) 1331, 1906, 3290, 4327, 5421
Msp20I TGGCCA 4 cut(s) 192, 2735, 3527, 5107
MspA1I CMGCKG 5 cut(s) 92, 2820, 4430, 4745, 5201
MspI CCGG 7 cut(s) 109, 120, 167, 1295, 3300, 4212, 4597
MunI CAATTG 5 cut(s) 2427, 2588, 4371, 4728, 4755
Mva1269I GAATGC 8 cut(s) 182, 966, 1201, 2572, 3173, 5284, 6018, 6618
MvaI CCWGG 9 cut(s) 467, 640, 773, 1196, 1727, 2501, 3792, 4481, 6243
MvnI CGCG 1 cut(s) 2189
NciI CCSGG 2 cut(s) 168, 1295
NcoI CCATGG 2 cut(s) 1458, 2771
NdeI CATATG 1 cut(s) 5964
NheI GCTAGC 1 cut(s) 4562
NlaIV GGNNCC 9 cut(s) 258, 505, 1891, 2932, 3262, 3730, 3796, 4601, 6037
NmuCI GTSAC 7 cut(s) 42, 796, 824, 1850, 2024, 2231, 6487
NsiI ATGCAT 3 cut(s) 2572, 2680, 5188
NspI RCATGY 6 cut(s) 365, 373, 1171, 1875, 4150, 5637
PaeI GCATGC 1 cut(s) 1171
PaeR7I CTCGAG 2 cut(s) 2316, 3308
PagI TCATGA 2 cut(s) 5335, 5718
PciSI GCTCTTC 2 cut(s) 4077, 4690
PcsI WCGNNNNNNNCGW 1 cut(s) 1808
PctI GAATGC 8 cut(s) 182, 966, 1201, 2572, 3173, 5284, 6018, 6618
PdmI GAANNNNTTC 5 cut(s) 1641, 4219, 4701, 4986, 6162
PflMI CCANNNNNTGG 2 cut(s) 3523, 5103
PfoI TCCNGGA 1 cut(s) 1194
PleI GAGTC 4 cut(s) 20, 1573, 1627, 2021
PpsI GAGTC 4 cut(s) 20, 1573, 1627, 2021
Ppu21I YACGTR 2 cut(s) 6399, 6578
PshAI GACNNNNGTC 1 cut(s) 567
PshBI ATTAAT 5 cut(s) 1998, 3077, 4422, 5645, 5937
PsiI TTATAA 1 cut(s) 1538
Psp124BI GAGCTC 4 cut(s) 1318, 3880, 4072, 5883
Psp6I CCWGG 9 cut(s) 465, 638, 771, 1194, 1725, 2499, 3790, 4479, 6241
PspFI CCCAGC 2 cut(s) 4439, 5810
PspGI CCWGG 9 cut(s) 465, 638, 771, 1194, 1725, 2499, 3790, 4479, 6241
PspN4I GGNNCC 9 cut(s) 258, 505, 1891, 2932, 3262, 3730, 3796, 4601, 6037
PspPI GGNCC 8 cut(s) 164, 937, 2044, 4599, 5401, 5922, 6335, 6603
PspXI VCTCGAGB 1 cut(s) 2316
PsrI GAACNNNNNNTAC 2 cut(s) 3244, 3276
PstI CTGCAG 3 cut(s) 652, 2449, 2494
PstNI CAGNNNCTG 5 cut(s) 2453, 3584, 4748, 5120, 5524
PsuI RGATCY 8 cut(s) 1713, 2095, 2924, 2930, 3728, 3763, 5888, 6569
PvuII CAGCTG 3 cut(s) 4430, 4745, 5201
RseI CAYNNNNRTG 5 cut(s) 1331, 1906, 3290, 4327, 5421
SacI GAGCTC 4 cut(s) 1318, 3880, 4072, 5883
SapI GCTCTTC 2 cut(s) 4077, 4690
Sau96I GGNCC 8 cut(s) 164, 937, 2044, 4599, 5401, 5922, 6335, 6603
ScaI AGTACT 5 cut(s) 579, 2535, 3048, 5243, 6370
SchI GAGTC 4 cut(s) 20, 1574, 1628, 2021
SexAI ACCWGGT 4 cut(s) 1725, 3790, 4479, 6241
SfcI CTRYAG 5 cut(s) 648, 2445, 2490, 2550, 4431
Sfr274I CTCGAG 2 cut(s) 2316, 3308
SinI GGWCC 6 cut(s) 164, 4599, 5401, 5922, 6335, 6603
SlaI CTCGAG 2 cut(s) 2316, 3308
SmiMI CAYNNNNRTG 5 cut(s) 1331, 1906, 3290, 4327, 5421
SmlI CTYRAG 9 cut(s) 854, 1451, 2175, 2316, 3050, 3308, 3329, 3769, 4649
SmoI CTYRAG 9 cut(s) 854, 1451, 2175, 2316, 3050, 3308, 3329, 3769, 4649
SpeI ACTAGT 1 cut(s) 4831
SphI GCATGC 1 cut(s) 1171
SspI AATATT 6 cut(s) 490, 714, 1225, 3957, 4281, 4840
SstI GAGCTC 4 cut(s) 1318, 3880, 4072, 5883
StyI CCWWGG 6 cut(s) 193, 1458, 2771, 2935, 4299, 6351
TaiI ACGT 4 cut(s) 409, 702, 6401, 6580
TaqI TCGA 8 cut(s) 2317, 2798, 3309, 3923, 4512, 6343, 6501, 6591
TaqII GACCGA 2 cut(s) 270, 2711
TatI WGTACW 8 cut(s) 577, 1558, 2071, 2533, 3046, 4336, 5241, 6368
TauI GCSGC 4 cut(s) 92, 533, 2189, 5903
TseFI GTSAC 7 cut(s) 42, 796, 824, 1850, 2024, 2231, 6487
Tsp45I GTSAC 7 cut(s) 42, 796, 824, 1850, 2024, 2231, 6487
TspGWI ACGGA 1 cut(s) 6133
Van91I CCANNNNNTGG 2 cut(s) 3523, 5103
VpaK11BI GGWCC 6 cut(s) 164, 4599, 5401, 5922, 6335, 6603
VspI ATTAAT 5 cut(s) 1998, 3077, 4422, 5645, 5937
XagI CCTNNNNNAGG 1 cut(s) 6284
XapI RAATTY 9 cut(s) 128, 201, 344, 1641, 3574, 4105, 4216, 5710, 5834
XceI RCATGY 6 cut(s) 365, 373, 1171, 1875, 4150, 5637
XcmI CCANNNNNNNNNTGG 1 cut(s) 1905
XhoI CTCGAG 2 cut(s) 2316, 3308
XmiI GTMKAC 2 cut(s) 217, 6525
XmnI GAANNNNTTC 5 cut(s) 1641, 4219, 4701, 4986, 6162
ZrmI AGTACT 5 cut(s) 579, 2535, 3048, 5243, 6370
Zsp2I ATGCAT 3 cut(s) 2572, 2680, 5188
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.